CN107114555A - A kind of bionic enzymatic prepares the production method of wheat Gly-His-Lys - Google Patents
A kind of bionic enzymatic prepares the production method of wheat Gly-His-Lys Download PDFInfo
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- CN107114555A CN107114555A CN201710296385.4A CN201710296385A CN107114555A CN 107114555 A CN107114555 A CN 107114555A CN 201710296385 A CN201710296385 A CN 201710296385A CN 107114555 A CN107114555 A CN 107114555A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/18—Vegetable proteins from wheat
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
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Abstract
The invention discloses the production method that a kind of bionic enzymatic from wheat gluten produces wheat Gly-His-Lys.It is characterized in that using wheat gluten as starting material, by bionic enzymatic, high speed centrifugation, ultrafiltration, electrodialysis, concentrate, be dried to obtain wheat Gly-His-Lys.The wheat Gly-His-Lys total protein content more than 90%, the soluble small-molecular peptides content of trichloroacetic acid more than 85%, with meet absorption of human body selection mechanism, have no toxic side effect, low cost and other advantages, can be applied in food, health food and medicine.
Description
Technical field
The present invention relates to a kind of preparation method of wheat Gly-His-Lys, and in particular to one kind bionic enzymatic from wheat gluten is produced
The industrial process of wheat Gly-His-Lys.
Background technology
Wheat peptide is a kind of vegetalitas biologically active peptide from wheat gluten, contains 15 kinds of amino needed by human
Acid.The features such as wheat protein peptide has good water solubility, easy absorption, bioactivity is strong.Research has shown that, the protein of human body intake
It is not to be absorbed in the form of amino acid after the enzyme effect in own digestive system, and is more in the form of low peptide
Absorbed.Nutrition and energy necessary to many protein peptides can not only provide growth in humans's development, also there is raising immunity of organism
Power, hypotensive, protolysate or the bioactive peptide food prepared by protolysate, with easy to digest, easy absorption, antiallergy
The features such as property.Because its own distinctive amino acid is constituted so that its dissolubility is low, limits the application of wheat protein peptide,
Water-soluble preferably biologically active peptide can be obtained by Enzymatic Hydrolysis of Wheat Gluten.The application of wheat gluten can be carried
The utilization rate of accessory substance in high wheat deep processing, improves the added value of wheat products.The yield of wheat in the world is big, uses albumen
Enzyme hydrolysis wheat gluten, prepares biologically active peptide, and cost is low, security is good, is easy to industrialized production, with boundless hair
Exhibition prospect.
The preparation method patent of existing wheat peptide has single enzyme to digest, and such as CN201110225461.5 is to use alkali protease
Digest etc.；There are double enzymes to digest, such as CN201110084121.5 is to be digested with alkali protease with papain,
CN201410681572.0 is is digested with cellulase with zytase, and CN201310535932.1 is to use neutral proteinase
Digested with glutamine transaminage；There is multienzyme enzymolysis, such as CN201510208320.0 is to carry out a step with alkali protease
Enzymolysis, two steps and three steps enzymolysis etc. are carried out with the complex enzyme being made up of alkali protease, neutral proteinase and flavor protease.
Bionic enzymatic be simulate oral protein pipe intestinal digesting process (stomach be Digestive site, absorb seldom, enteron aisle is
Digestion, absorption site), the creative method that protide is digested into small-molecule peptide, i.e., under suitable condition with stomach egg
White enzyme, trypsase carry out continuous enzymolysis, then obtain small with relative molecular mass through high speed centrifugation, ultrafiltration, electrodialysis or nanofiltration
In the small-molecule peptide based on 3KD.Compared with conventional art, the active materials of absorbed into serum are identical with direct oral protein,
Bionic enzymatic extracts active ingredients are more abundant, and bioavilability is high, not only increases curative effect, and eliminate and take M-band etc.
Caused immunogene reaction, reduces adverse reaction rate, safe；Compared with other enzyme solutions, bionic enzymatic is more
Meet the selection mechanism of human body, it is safer.
The inventive method that wheat Gly-His-Lys are inventors, the work of amplifiedization are produced from wheat gluten in bionic enzymatic method
Industry production checking, it is stable, feasible, have broad application prospects.
The content of the invention
The first object of the present invention is to provide the industry life that a kind of bionic enzymatic from wheat gluten produces wheat Gly-His-Lys
Production method；Second purpose is to provide the wheat Gly-His-Lys obtained by described industrialized preparing process；3rd purpose is to carry
Take application of the described wheat Gly-His-Lys in food, health food and medicine field.
The object of the present invention is achieved like this：
A kind of bionic enzymatic from wheat gluten produces the industrial process of wheat Gly-His-Lys, using following steps：
(1) add purified water 20-40 liters into bionic enzymatic tank, start stirring slurry, 30-60 revs/min of rotating speed is set, taken small
Aleuronat powder 2kg, is slowly added to, and addition continues to stir swollen 30 minutes after finishing, and interlayer is logical to be steam heated to 80-85 DEG C of sterilizing
15-30 minutes, interlayer led to cooling and is water-cooled to about 50-55 DEG C of temperature in tank, starts intermediate water and is circulated with heater, heater adds
Hot to 50-55 DEG C insulation；
(2) start pH detection terminals, watery hydrochloric acid regulation pH value 1.8-2.2 is added with peristaltic pump into bionic enzymatic tank, plus
Enter the 1-3% pepsins of wheat gluten amount, insulation enzymolysis 1-3 hours monitors pH value at any time, and supplement watery hydrochloric acid keeps pH value to exist
1.8-2.2 in the range of；
(3) 40% sodium hydroxide regulation pH value 7.8-8.2 is added with peristaltic pump into bionic enzymatic tank, adds 1-3% pancreases
Protease, insulation enzymolysis 3-6 hours monitors pH value at any time, and 40% sodium hydroxide of supplement keeps pH value in the range of 7.8-8.2；
(4) watery hydrochloric acid regulation pH value 6.8-7.2 is added with peristaltic pump into bionic enzymatic tank, bleeds off intermediate water, interlayer leads to
Steam, which boils, is heated to 80-85 DEG C of enzyme 15-30 minutes of going out, tapping；
(5) centrifuged into tubular-bowl centrifuge, 8000~12000 revs/min of rotating speed, supernatant passes through 1812 type ultrafiltration
Device ultrafiltration, milipore filter relative molecular mass is 3KD, and permeate is through electrodialysis desalination, 30~90 volts of voltage, except saline solution is through decompression
The clear cream that 60 DEG C of relative densities are 1.10~1.15 is concentrated into, spray drying, EAT is 180-200 DEG C, leaving air temp is
90-100 DEG C, obtain wheat Gly-His-Lys, compound membrane bag packaging.
Protein content is more than 90% in wheat gluten described in step (1), and moisture is less than 10%；
Pepsin enzyme activity described in step (2) is 3000U/g；
Trypsase enzyme activity described in step (3) is 100000U/g；
Total protein content is more than 90% in wheat Gly-His-Lys described in step (5), the soluble small molecule of trichloroacetic acid
Peptide content is more than 85%, and free aminoacid content is less than 5%, and moisture is less than 7%.
A kind of wheat Gly-His-Lys, are obtained by above-mentioned industrialized preparing process.The wheat Gly-His-Lys obtained using this method,
Its main relative molecular mass is below 3KD, and with extremely strong water solubility, its total protein content is more than 90%, trichloroacetic acid
Soluble small-molecular peptides content is more than 85%, and free aminoacid content is less than 5%, and moisture is less than 7%.
Above-mentioned bionic enzymatic tank and enzymatic hydrolysis system refer to the equipment being related in CN205874433U, CN205590709U,
With the increase of volume of equipment, the consumption of each material can increase therewith in the inventive method.There is setting for similar function at other
Standby upper, this method is equally adapted to.
Materials of wheat albumen powder in the present invention, can be available from the finished product raw material of in the market or first from wheat
Reclaimed water solution obtains gluten powder or the fluid product containing wheat gluten.In a word, no matter with which kind of raw material feed intake production (wheat,
Gluten powder, wheat gluten liquid etc.), as long as being related to the content of the invention protected in production technology, that is, it is considered as and employs
The technology contents of the present invention.
Application of the wheat Gly-His-Lys obtained by above-mentioned industrial process in food, health food and medicine, including but
It is not limited to following applications：According to nutritive peculiarity, functional characteristic and the processing characteristics of the wheat Gly-His-Lys produced, made with wheat Gly-His-Lys
For basic material, many high-quality, nutrition, the food of health can be developed.Suppress angiotensin-converter according to wheat Gly-His-Lys
The characteristics of enzyme (ACE) is active, can develop functional food and health food, the medicine of hypotensive；Cancer is drawn using its suppression sea
The characteristic of the growth of cell, can develop functional food and health food, the medicine of cancer suppressing action；Utilize its aggegation and anti-freezing
The activity of collection, can develop anti-hemagglutination, the food and health food of promoting blood circulation；Promote protein synthesis and anti-tired using it
The characteristic of labor, can develop the movement nutrition food and health food of enhancing muscle and dispelling fatigue；Immunological regulation is proposed using it
With oxidation resistant characteristic, the functional food for improving immunity of organisms and anti-aging can be developed；Using good water solubility, easily absorb,
The strong characteristic of bioactivity, can make medical liquid food etc..
The beneficial effects of the invention are as follows：Present invention bionic enzymatic from wheat gluten produces the industrial production side of wheat Gly-His-Lys
Method, meets human body selection mechanism, and preparation technology is easy, feasible, and cost is low, production process environmental protection；The wheat Gly-His-Lys of gained
Total protein content is more than 90%, and small-molecule peptide content is more than 85%, and relative molecular mass is nontoxic mainly in below 3KD
Side effect, reaches the pure natural requirement of pollution-free food, can obtain good economic benefit.
Embodiment
Embodiment is set forth below, the present invention is further illustrated, each embodiment is merely to illustrate the present invention, is not intended to limit this hair
It is bright：
The present embodiment of embodiment 1 comprises the following steps：
(1) add 20 liters of purified water into bionic enzymatic tank, start stirring slurry, 60 revs/min of rotating speed is set, wheat gluten is taken
(wheat separation protein, protein content is more than 90%, and moisture is less than 10%) 2kg, is slowly added to, and addition continues to stir after finishing for powder
Mix swollen 30 minutes, interlayer is logical to be steam heated to 80-85 DEG C and sterilize 15 minutes, and interlayer leads to cooling and is water-cooled to temperature about 55 in tank
DEG C, start intermediate water and circulated with heater, heater is heated to 55 DEG C of insulations；
(2) start pH detection terminals, watery hydrochloric acid regulation pH value 2.0 is added with peristaltic pump into bionic enzymatic tank, added small
1% pepsin (enzyme activity is 3000U/g) of aleuronat amount, insulation enzymolysis 1 hour, monitors pH value at any time, supplements watery hydrochloric acid
PH value is kept in the range of 1.8-2.2；
(3) 40% sodium hydroxide regulation pH value 8.0 is added with peristaltic pump into bionic enzymatic tank, adds 1% trypsase
(enzyme activity is 100000U/g), insulation enzymolysis 3 hours monitors pH value at any time, and 40% sodium hydroxide of supplement keeps pH value in 7.8-
8.2 in the range of；
(4) watery hydrochloric acid regulation pH value 6.8 is added with peristaltic pump into bionic enzymatic tank, bleeds off intermediate water, interlayer leads to steam
Boil and be heated to the 80-85 DEG C of enzyme 30 minutes of going out, tapping；
(5) centrifuged into tubular-bowl centrifuge, 8000~12000 revs/min of rotating speed, supernatant passes through 1812 type ultrafiltration
Device ultrafiltration, milipore filter relative molecular mass is 3KD, and permeate is through electrodialysis desalination, 30 volts of voltage, except saline solution is through being concentrated under reduced pressure
The clear cream for being 1.10~1.15 to 60 DEG C of relative densities, spray drying, EAT is 180-200 DEG C, and leaving air temp is 90-
100 DEG C, obtain wheat Gly-His-Lys, compound membrane bag packaging.
The present embodiment of embodiment 2 comprises the following steps：
(1) add 40 liters of purified water into bionic enzymatic tank, start stirring slurry, 30 revs/min of rotating speed is set, wheat gluten is taken
Powder 2kg, is slowly added to, and addition continues to stir swollen 30 minutes after finishing, and interlayer is logical to be steam heated to 80-85 DEG C of 15 points of sterilizing
Clock, interlayer leads to cooling and is water-cooled to about 50 DEG C of temperature in tank, starts intermediate water and is circulated with heater, heater is heated to 50 DEG C of guarantors
Temperature；
(2) start pH detection terminals, watery hydrochloric acid regulation pH value 1.8 is added with peristaltic pump into bionic enzymatic tank, added small
3% pepsin of aleuronat amount, insulation enzymolysis 3 hours monitors pH value at any time, and supplement watery hydrochloric acid keeps pH value in 1.8-2.2
In the range of；
(3) 40% sodium hydroxide regulation pH value 8.2 is added with peristaltic pump into bionic enzymatic tank, adds 3% trypsase,
Insulation enzymolysis 6 hours, monitors pH value at any time, and 40% sodium hydroxide of supplement keeps pH value in the range of 7.8-8.2；
(4) watery hydrochloric acid regulation pH value 7.2 is added with peristaltic pump into bionic enzymatic tank, bleeds off intermediate water, interlayer leads to steam
Boil and be heated to the 80-85 DEG C of enzyme 15 minutes of going out, tapping；
(5) centrifuged into tubular-bowl centrifuge, 8000~12000 revs/min of rotating speed, supernatant passes through 1812 type ultrafiltration
Device ultrafiltration, milipore filter relative molecular mass is 3KD, and permeate is through electrodialysis desalination, 90 volts of voltage, except saline solution is through being concentrated under reduced pressure
The clear cream for being 1.10~1.15 to 60 DEG C of relative densities, spray drying, EAT is 180-200 DEG C, and leaving air temp is 90-
100 DEG C, obtain wheat Gly-His-Lys, compound membrane bag packaging.
The wheat Gly-His-Lys of embodiment 3：
The wheat Gly-His-Lys that industrialized preparing process described in Example 1 is obtained, after testing：Its total protein content is
91%, the soluble small-molecular peptides content of trichloroacetic acid is 88%, and free aminoacid content is 3.2%, and moisture is
5.2%, relative molecular weight distribution is：
Product prepared by the wheat Gly-His-Lys of embodiment 4：
Wheat Gly-His-Lys 400g obtained by the method for Example 1, by wheat Gly-His-Lys:Corn oil:Beeswax:Lecithin is
10:36:3:1 mixed material, is ground 30 minutes through colloid mill, and 100 are pressed with gelatin, glycerine, purified water, iron oxide black：40：100：
1 ratio making soft capsule hide glue gelatin, 55 ± 5 DEG C of control glue box temperature, 40 ± 5 DEG C of heat insulation tank, 36 ± 5 DEG C of sprinkler body temperature, with
Pellet press suppresses soft capsule, per the tolerant heavy 0.50g of intragranular, 18 DEG C~26 DEG C cold air drying 4h (relative humidity is 30%~40%)
Sizing, washes ball, drying, selects ball, packaging, be prepared into soft capsule finished product.
The application of the wheat Gly-His-Lys of embodiment 5：
Suppress the characteristics of angiotensin converting enzyme (ACE) is active according to wheat Gly-His-Lys, the function of hypotensive can be developed
Property food and health food, medicine；The characteristic of the growth of cancer cell is drawn using its suppression sea, the function of cancer suppressing action can be developed
Property food and health food, medicine；Using its aggegation and antiagglutinating activity, anti-hemagglutination, the food of promoting blood circulation can be developed
Product and health food；Promote protein synthesis and antifatigue characteristic using it, enhancing muscle and dispelling fatigue can be developed
Movement nutrition food and health food；Immunological regulation and oxidation resistant characteristic are proposed using it, raising immunity of organisms can be developed
With the functional food of anti-aging；Using good water solubility, the easily strong characteristic of absorption, bioactivity, medical liquid food etc. can be made.
Claims (5)
1. a kind of bionic enzymatic prepares the production method of wheat Gly-His-Lys, it is characterised in that use following steps：
（1）Add purified water 20-40 liters into bionic enzymatic tank, start stirring slurry, 30-60 revs/min of rotating speed is set, wheat egg is taken
White powder 2kg, is slowly added to, and addition continues to stir swollen 30 minutes after finishing, and interlayer is logical to be steam heated to 80-85 DEG C of sterilizing 15-
30 minutes, interlayer led to cooling and is water-cooled to about 50-55 DEG C of temperature in tank, starts intermediate water and is circulated with heater, heater heating
To 50-55 DEG C of insulation；
（2）Start pH detection terminals, watery hydrochloric acid regulation pH value 1.8-2.2 is added with peristaltic pump into bionic enzymatic tank, added small
The 1-3% pepsins of aleuronat amount, insulation enzymolysis 1-3 hours monitors pH value at any time, and supplement watery hydrochloric acid keeps pH value in 1.8-
2.2 in the range of；
（3）40% sodium hydroxide regulation pH value 7.8-8.2 is added with peristaltic pump into bionic enzymatic tank, 1-3% trypsase is added,
Insulation enzymolysis 3-6 hours, monitors pH value at any time, and 40% sodium hydroxide of supplement keeps pH value in the range of 7.8-8.2；
（4）Watery hydrochloric acid regulation pH value 6.8-7.2 is added with peristaltic pump into bionic enzymatic tank, intermediate water is bled off, interlayer leads to steam
Boil and be heated to 80-85 DEG C of enzyme 15-30 minutes of going out, tapping；
（5）Centrifuged into tubular-bowl centrifuge, 8000-12000 revs/min of rotating speed, supernatant passes through 1812 type ultrafiltration apparatus
Ultrafiltration, milipore filter relative molecular mass is 3KD, and permeate is through electrodialysis desalination, voltage 30-90 volts, except saline solution is through being concentrated under reduced pressure
The clear cream for being 1.10-1.15 to 60 DEG C of relative densities, spray drying, EAT is 180-200 DEG C, and leaving air temp is 90-100
DEG C, obtain wheat Gly-His-Lys, compound membrane bag packaging.
2. bionic enzymatic according to claim 1 prepares the production method of wheat Gly-His-Lys, it is characterised in that described stomach egg
White enzyme enzyme activity is 3000U/g；Trypsase enzyme activity is 100000U/g；Protein content is more than 90% in wheat gluten, water
Divide and be less than 10%.
3. bionic enzymatic according to claim 1 prepares the production method of wheat Gly-His-Lys, it is characterised in that resulting is small
Total protein content is more than 90% in wheat Gly-His-Lys, and the soluble small-molecular peptides content of trichloroacetic acid is more than 85%, free amino acid
Content is less than 5%, and moisture is less than 7%.
4. a kind of wheat Gly-His-Lys, are obtained by the industrialized preparing process described in claim 1-3.
5. application of the wheat Gly-His-Lys in food, health food and medicine field described in claim 4.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676835A (en) * | 2018-05-25 | 2018-10-19 | 山东禹泽医药科技有限公司 | A kind of preparation method of composite reactive Gly-His-Lys for Antialcoholic liver-protecting |
| CN112226478A (en) * | 2020-06-11 | 2021-01-15 | 杭州康源食品科技有限公司 | Wheat peptide and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101717805A (en) * | 2009-12-01 | 2010-06-02 | 江南大学 | Method for preparing wheat protein source opioid peptide by enzymolysis of wheat protein |
| CN102836416A (en) * | 2012-09-11 | 2012-12-26 | 凤台文亮置业有限公司 | Bionic enzymolysis preparation method of leech powder |
| CN205590709U (en) * | 2016-01-19 | 2016-09-21 | 安徽生物肽产业研究院有限公司 | Bionical enzymatic hydrolysis device |
| CN205874433U (en) * | 2016-01-19 | 2017-01-11 | 安徽生物肽产业研究院有限公司 | Intelligent bionical enzymolysis system |
-
2017
- 2017-04-28 CN CN201710296385.4A patent/CN107114555A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101717805A (en) * | 2009-12-01 | 2010-06-02 | 江南大学 | Method for preparing wheat protein source opioid peptide by enzymolysis of wheat protein |
| CN102836416A (en) * | 2012-09-11 | 2012-12-26 | 凤台文亮置业有限公司 | Bionic enzymolysis preparation method of leech powder |
| CN205590709U (en) * | 2016-01-19 | 2016-09-21 | 安徽生物肽产业研究院有限公司 | Bionical enzymatic hydrolysis device |
| CN205874433U (en) * | 2016-01-19 | 2017-01-11 | 安徽生物肽产业研究院有限公司 | Intelligent bionical enzymolysis system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676835A (en) * | 2018-05-25 | 2018-10-19 | 山东禹泽医药科技有限公司 | A kind of preparation method of composite reactive Gly-His-Lys for Antialcoholic liver-protecting |
| CN112226478A (en) * | 2020-06-11 | 2021-01-15 | 杭州康源食品科技有限公司 | Wheat peptide and preparation method and application thereof |
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