CN107114359A - The both-side opening storage bag and preparation method of animal tissue's sample in a kind of liquid nitrogen container - Google Patents
The both-side opening storage bag and preparation method of animal tissue's sample in a kind of liquid nitrogen container Download PDFInfo
- Publication number
- CN107114359A CN107114359A CN201710456489.7A CN201710456489A CN107114359A CN 107114359 A CN107114359 A CN 107114359A CN 201710456489 A CN201710456489 A CN 201710456489A CN 107114359 A CN107114359 A CN 107114359A
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- cotton
- bag
- liquid nitrogen
- sample
- big
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Hematology (AREA)
- Mechanical Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of both-side opening storage bag of animal tissue's sample in liquid nitrogen container, the storage bag includes the bag rope being connected in the bag and one end of rectangular platypelloid type with the bag.The bag is that the square cotton of small one and large one two block length constitutes and is divided into three layers, and its medium and small cotton is bottom and the center below big cotton, and big cotton is the second layer with small cotton lap, and the big symmetrical flap section of cotton is upper strata；The second layer and bottom connection shape some right-angled trapezium tiny pockets in a row；The second layer and the upper strata connect to form symmetrical two big pocket；The cryopreservation tube equipped with sample is placed in the tiny pocket.The present invention is simple in construction, be easy to make, easy to use, quick.
Description
Technical field
The present invention relates to animal sample low temperature field of storage, more particularly in a kind of liquid nitrogen container animal tissue's sample both sides
The storage bag that is open and preparation method.
Background technology
In animal science experimental study, many tissue samples need progress snap frozen to be preserved and used, liquid nitrogen
Freezen protective is the most frequently used snap frozen in scientific research and the method for preserving special material.
The liquid nitrogen cryogenics storage of animal tissue's sample is usually that will be equipped with cryopreservation tube of tissue sample etc. to be put into liquid nitrogen pail
In, it is common to improve liquid nitrogen container volume utilization when needing storing samples many because pail quantity and volume are extremely limited
Way is directly to lose sample in liquid nitrogen container, and such storing samples are increased, but when needing some sample, it is necessary to by liquid nitrogen
All samples in tank are poured out, and are found one by one, not only cause the waste of liquid nitrogen, and very inconvenient, time and effort consuming.
Therefore, it is necessary to which the demand of storing classifiedly can be met by inventing one kind, while can improve liquid nitrogen container space availability ratio again
Liquid nitrogen container storage accessory.
The content of the invention
The technical problems to be solved by the invention are to provide two of animal tissue's sample in a kind of conveniently liquid nitrogen container
Side opening storage bag.
Another technical problem to be solved by this invention is to provide the both-side opening of animal tissue's sample in the liquid nitrogen container
The preparation method of storage bag.
To solve the above problems, in a kind of liquid nitrogen container of the present invention animal tissue's sample both-side opening storage bag,
It is characterized in that：The storage bag includes the bag rope being connected in the bag and one end of rectangular platypelloid type with the bag；The bag
Constituted for the square cotton of small one and large one two block length and be divided into three layers, its medium and small cotton be bottom and below big cotton in
Centre, big cotton is the second layer with small cotton lap, and the big symmetrical flap section of cotton is upper strata；The second layer and the bottom
Layer connection shape some right-angled trapezium tiny pockets in a row；The second layer and the upper strata connect to form symmetrical two big mouth
Bag；The cryopreservation tube equipped with sample is placed in the tiny pocket.
The material of the bag is cotton, and its length is that the cryopreservation tube is high freezes the 2.5 of lid diameter sum with described
Times, its width and the number and liquid nitrogen container bore of the outer perimeter for freezing lid and animal tissue match.
The big cotton size is 2 times of the small cotton size；The length of the small cotton is more than the cryopreservation tube
High and thick head diameter sum, its width and the number and liquid nitrogen container bore of the cryopreservation tube thickness and animal tissue match.
The second layer and the upper strata joint are provided with interior broken line.
Linked together between the second layer and the upper strata by suture, the suture is in the right-angled trapezium
Right angle waist position.
Wide bottom length is more than the thickness of the cryopreservation tube in the right-angled trapezium, and its narrow bottom length is more than the cryopreservation tube
The 1/2 of bottom outer perimeter.
The tiny pocket quantity matches with gathering the number and liquid nitrogen container bore of tissue.
The material of the bag rope is hard cotton thread or the wire or combination that are wrapped up by cotton, its length and liquid nitrogen
Tank height and the number of the tiny pocket match.
The preparation method of the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as described above, including it is following
Step：
(1) intend the tissue types of sampling according to each animal individual, hold the size of the cryopreservation tube of tissue samples, determine that one is big by one
The size of the small square cotton of two block lengths, and cut cotton；
(2) small one and large one two pieces of described cottons are stacked, folded, form three layers；Wherein big cotton is positioned on small cotton
Side, the middle body that small cotton is located at big cotton is overlapping, and infolding is distinguished at big cotton two ends 1/4, and small cotton is bottom, big cotton
It is the second layer with small cotton lap, big cotton flap section is upper strata；
(3) move towards to carry out the second layer and the bottom suture of center section along right-angled trapezium, suture right-angled trapezium
Upper bottom length matches with the cryopreservation tube, forms the middle tiny pocket of the storage cryopreservation tube；
(4) the side on the upper strata, the second layer and the bottom is sutured, the trapezoidal high and cryopreservation tube phase
Match somebody with somebody, form tiny pocket and two big pocket that the cryopreservation tube is deposited in both sides；Pocket openings appear alternatively in the second layer and
The interior position of broken line of the upper strata joint；
(5) one end bag restricted is fixedly connected on the centre of any three layers of suture of bag by way of suturing or punching fixation
Position, its other end is used to paste sample label.
The present invention has advantages below compared with prior art：
1st, bag is that the square cotton of small one and large one two block length constitutes and is divided into three layers in the present invention, and its medium and small cotton is bottom and position
Center below big cotton, big cotton is the second layer with small cotton lap, and the big symmetrical flap section of cotton is upper strata；The
Two layers and bottom connection shape some right-angled trapezium tiny pockets in a row；The second layer and upper strata connect to form symmetrical two big mouth
Bag, not only meets the demand that sample is stored classifiedly in liquid nitrogen container, and improves liquid nitrogen container space availability ratio, while for liquid nitrogen
In tank during storage animal tissue's sample, selection sample is found convenient and swift.
2nd, the present invention is simple in construction, be easy to make, easy to use.
Brief description of the drawings
The embodiment to the present invention is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 is stacked for the bag of the present invention, is folded schematic diagram.
Fig. 2 is the positive schematic top plan view of the present invention.
Fig. 3 is back side schematic top plan view of the present invention.
Fig. 4 is schematic structural view of the invention.
In figure：1- upper stratas；The 2- second layers；3- bottoms；Broken line in 4-；5- sutures；6- bags of ropes；7- cryopreservation tubes.
Embodiment
As shown in Fig. 1 ~ 4, a kind of both-side opening storage bag of animal tissue's sample in liquid nitrogen container, the storage bag is included in length
The bag rope 6 that the bag of square platypelloid type and one end are connected with bag.
Bag is that the square cotton of small one and large one two block length constitutes and is divided into three layers, and its medium and small cotton is bottom 3 and positioned at big
Center below cotton, big cotton is the second layer 2 with small cotton lap, and the big symmetrical flap section of cotton is upper strata 1；Second
Layer 2 and the connection shape some right-angled trapezium tiny pockets in a row of bottom 3；The second layer 2 and the connection of upper strata 1 form symmetrical two big mouth
Bag；The cryopreservation tube 7 equipped with sample is placed in tiny pocket.
Wherein：The material of bag is the cotton for the gas of resistance to deep colling, and its length is the height of cryopreservation tube 8 and the lid diameter sum of cryopreservation tube 8
2.5 times, the outer perimeter and the number of animal tissue and liquid nitrogen container bore that its width is covered with cryopreservation tube 8 match.
Big cotton size is 2 times of small cotton size；The length of small cotton be more than cryopreservation tube 7 high and thick head diameter it
With its width and the number and liquid nitrogen container bore of the thickness of cryopreservation tube 7 and animal tissue match.
The second layer 2 and the joint of upper strata 1 are provided with interior broken line 4.
Linked together between the second layer 2 and upper strata 1 by suture 5, the suture 5 is in the right angle waist of right-angled trapezium
Position.
Wide bottom length is more than the thickness of cryopreservation tube 7 in right-angled trapezium, and its narrow bottom length is more than the bottom outer perimeter of cryopreservation tube 7
1/2.
Tiny pocket quantity matches with gathering the number and liquid nitrogen container bore of tissue.
The material of bag rope 6 is the wire or combination for being difficult the hard cotton thread of winding or being wrapped up by cotton, is used for
Tie up, draw storage bag；The number of its length and liquid nitrogen container height and the tiny pocket matches.
Cryopreservation tube 7 can also be replaced with centrifuge tube.
The preparation method of the both-side opening storage bag of animal tissue's sample, comprises the following steps in the liquid nitrogen container：
(1) intend the tissue types of sampling according to each animal individual, hold the size of the cryopreservation tube 7 of tissue samples, determine that one is big by one
The size of the small square cotton of two block lengths, and cut cotton；
(2) small one and large one two pieces of described cottons are stacked, folded, form three layers；Wherein big cotton is positioned on small cotton
Side, the middle body that small cotton is located at big cotton is overlapping, and infolding is distinguished at big cotton two ends 1/4, and small cotton is bottom 3, big cotton
Cloth is the second layer 2 with small cotton lap, and big cotton flap section is upper strata 1；
(3) move towards to carry out the second layer 2 and bottom 3 above and below the suture of center section, the right-angled trapezium of suture 5 along right-angled trapezium
Bottom length matches with cryopreservation tube 7, forms the middle tiny pocket of storage cryopreservation tube 7；
(4) the side on upper strata 1, the second layer 2 and bottom 3 is sutured, trapezoidal height matches with cryopreservation tube 7, form both sides and deposit
Put the tiny pocket and two big pocket of cryopreservation tube 7；Pocket openings appear alternatively in the interior broken line 4 of the second layer 2 and the joint of upper strata 1
Position；
(5) one end of bag rope 6 is fixedly connected in any three layers of suture 5 of bag by way of suturing or punching is fixed
Between position, its other end is used to paste and can record sample message on sample label, label, for localizing sample, is easy to sample
Find.
Embodiment makes six kinds of animal tissue's samples of a storage, and sample is put into after being fitted into 1.5mL eppendorf centrifuge tubes
The storage bag, the high 4.0cm of centrifuge tube, lid the widest part 2.0cm, liquid nitrogen tank volume are 10L, interior high 45.0cm.
According to each animal individual intend sampling tissue types, hold tissue samples eppendorf centrifuge tubes it is big
It is small, the size a length of 13.5cm, a width of 5cm, size a length of 13.5cm, a width of 10cm of big cotton of small cotton are determined, is then cut out
Cut size cotton；
(2) small one and large one two pieces of described cottons are stacked, folded, form three layers；Wherein big cotton is positioned on small cotton
Side, the middle body that small cotton is located at big cotton is overlapping, and big cotton two ends 2.5cm distinguishes infolding everywhere, and small cotton is bottom 3,
Big cotton is the second layer 2 with small cotton lap, and big cotton flap section is upper strata 1；
(3) move towards to carry out the second layer 2 and bottom 3 suture of center section along right-angled trapezium, trapezoidal wide bottom is 3.0cm, and narrow bottom is
1.5cm, forms the middle tiny pocket of storage eppendorf centrifuge tubes；
(4) the side on upper strata 1, the second layer 2 and bottom 3 is sutured, trapezoidal height matches with eppendorf centrifuge tubes, shape
The tiny pocket and two big pocket of eppendorf centrifuge tubes are deposited into both sides；Pocket openings appear alternatively in the second layer 2 and upper strata
The position of interior broken line 4 of 1 joint；
(5) length is fixedly connected for 65cm one end for being difficult to wind hard cotton bag rope 6 by way of suturing or punching is fixed
The centre position on the left of bag, its other end is used to paste that sample message can be recorded on sample label, label, for positioning sample
Product, are easy to sample to find.
The present invention is in use, mark the organization name in the cryopreservation tube 7 being housed in the narrow bottom position of each tiny pocket of bag
Claim, the cryopreservation tube 7 that will be equipped with the sample of tissue is respectively put into tiny pocket, upper strata 1 is turned down, and seals the opening of tiny pocket, use
Bag rope is tied up（It can not also tie up）Afterwards, it is put into liquid nitrogen container, the other end of bag rope 6 sticks the tag number of animal individual and indicated and deposits
The tissue samples title put, is stayed in outside liquid nitrogen container.
Described above is only a case study on implementation to the present invention, not makees any formal limit to the present invention
System, any simple modification that every technical spirit according to the present invention is made to embodiment of above, equivalent variations and modification,
Belong in the range of technical solution of the present invention.
Claims (9)
1. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container, it is characterised in that：The storage bag is included in rectangular
The bag rope that the bag of platypelloid type and one end are connected with the bag（6）；The bag is the square cotton structure of small one and large one two block length
Into and be divided into three layers, its medium and small cotton is bottom（3）And the center below big cotton, big cotton and small cotton lap
For the second layer（2）, the big symmetrical flap section of cotton is upper strata（1）；The second layer（2）With the bottom（3）Connection forms one
Arrange some right-angled trapezium tiny pockets；The second layer（2）With the upper strata（1）Connection forms symmetrical two big pocket；It is described
The cryopreservation tube equipped with sample is placed in tiny pocket（7）.
2. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as claimed in claim 1, it is characterised in that：Institute
The material for stating bag is cotton, and its length is the cryopreservation tube（8）The high and cryopreservation tube（8）2.5 times of lid diameter sum, its
Width and the cryopreservation tube（8）The outer perimeter of lid and the number of animal tissue and liquid nitrogen container bore match.
3. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as claimed in claim 1, it is characterised in that：Institute
It is 2 times of the small cotton size to state big cotton size；The length of the small cotton is more than the cryopreservation tube（7）It is high with thick head
Diameter sum, its width and the cryopreservation tube（7）Thickness and the number and liquid nitrogen container bore of animal tissue match.
4. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as claimed in claim 1, it is characterised in that：Institute
State the second layer（2）With the upper strata（1）Joint is provided with interior broken line（4）.
5. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as claimed in claim 1, it is characterised in that：Institute
State the second layer（2）With the upper strata（1）Between pass through suture（5）Link together, the suture（5）In right angle ladder
The right angle waist position of shape.
6. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as claimed in claim 1, it is characterised in that：Institute
State wide bottom length in right-angled trapezium and be more than the cryopreservation tube（7）Thickness, its narrow bottom length be more than the cryopreservation tube（7）Bottom
The 1/2 of outer perimeter.
7. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as claimed in claim 1, it is characterised in that：Institute
State tiny pocket quantity and match with gathering the number and liquid nitrogen container bore of tissue.
8. the both-side opening storage bag of animal tissue's sample in a kind of liquid nitrogen container as claimed in claim 1, it is characterised in that：Institute
State bag rope（6）Material be hard cotton thread or the wire or combination that are wrapped up by cotton, its length and liquid nitrogen container height
And the number of the tiny pocket matches.
9. the preparation method of the both-side opening storage bag of animal tissue's sample, bag in a kind of liquid nitrogen container as claimed in claim 1
Include following steps：
(1) intend the tissue types of sampling according to each animal individual, hold the cryopreservation tube of tissue samples（7）Size, determine that one is big
The size of the one small square cotton of two block lengths, and cut cotton；
(2) small one and large one two pieces of described cottons are stacked, folded, form three layers；Wherein big cotton is positioned on small cotton
Side, the middle body that small cotton is located at big cotton is overlapping, and infolding is distinguished at big cotton two ends 1/4, and small cotton is bottom（3）, greatly
Cotton is the second layer with small cotton lap（2）, big cotton flap section is upper strata（1）；
(3) along right-angled trapezium trend to the second layer（2）With the bottom（3）Carry out the suture of center section, suture（5）
The upper bottom length of right-angled trapezium and the cryopreservation tube（7）Match, form the storage cryopreservation tube（7）Middle tiny pocket；
(4) to the upper strata（1）, the second layer（2）With the bottom（3）Side sutured, it is trapezoidal high to freeze with described
Deposit pipe（7）Match, form both sides and deposit the cryopreservation tube（7）Tiny pocket and two big pocket；Pocket openings are alternately present
In the second layer（2）With the upper strata（1）The interior broken line of joint（4）Position；
(5) bag is restricted（6）One end be fixedly connected on any three layers of suture of bag by way of suturing or punching is fixed（5）
Centre position, its other end be used for paste sample label.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710456489.7A CN107114359B (en) | 2017-06-16 | 2017-06-16 | Storage bag with openings at two sides for animal tissue samples in liquid nitrogen tank and manufacturing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710456489.7A CN107114359B (en) | 2017-06-16 | 2017-06-16 | Storage bag with openings at two sides for animal tissue samples in liquid nitrogen tank and manufacturing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107114359A true CN107114359A (en) | 2017-09-01 |
| CN107114359B CN107114359B (en) | 2020-12-08 |
Family
ID=59719760
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710456489.7A Active CN107114359B (en) | 2017-06-16 | 2017-06-16 | Storage bag with openings at two sides for animal tissue samples in liquid nitrogen tank and manufacturing method |
Country Status (1)
| Country | Link |
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| CN (1) | CN107114359B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318276A (en) * | 2018-01-09 | 2018-07-24 | 中国农业科学院棉花研究所 | A kind of osculum liquid nitrogen container Special sample collection device and application method |
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| US4251995A (en) * | 1979-04-25 | 1981-02-24 | Hedbergska Stiftelsen | Method of freezing human blood platelets in glycerol-glucose using a statically controlled cooling rate device |
| US4681839A (en) * | 1982-09-23 | 1987-07-21 | Swartz Mitchell R | Systems to preserve living tissue |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN107114359B (en) | 2020-12-08 |
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