CN107102129A - A kind of ELIASA - Google Patents
A kind of ELIASA Download PDFInfo
- Publication number
- CN107102129A CN107102129A CN201710396758.5A CN201710396758A CN107102129A CN 107102129 A CN107102129 A CN 107102129A CN 201710396758 A CN201710396758 A CN 201710396758A CN 107102129 A CN107102129 A CN 107102129A
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- signal
- plate
- signal plate
- micropore
- eliasa
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
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- Hematology (AREA)
- General Physics & Mathematics (AREA)
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- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention proposes a kind of ELIASA, measuring samples in ELISA Plate micropore are arranged in mounting bracket, the light beam that light source assembly is sent passes through measuring samples, secondary signal plate collects corresponding optical signal, and by corresponding optical signal transmission to the first coupled signal plate, signal acquisition is carried out by signal acquisition module is unified.Because the signal on the first signal plate can be gathered disposably by signal acquisition module simultaneously, so as to avoid the ageing difference that signal acquisition occurs, it is to avoid error occurs in testing result, while improving detection efficiency；And with simple in construction, occupy little space, the advantages of with low cost.
Description
Technical field
The present invention relates to enzyme linked immunosorbent detection field, more particularly to a kind ELIASA.
Background technology
Enzyme linked immunosorbent detection technology is that the principle reacted based on antibody antigen carries out the inspection of quantitative and qualitative analysis to determinand
Survey method.Enzyme linked immunosorbent detection technology has the advantages that high specificity, sensitivity are high, easy, is the important of modern life science
Research meanses, have a wide range of applications in bioanalysis detection field.
ELIASA is then a kind of instrument for carrying out enzyme linked immunosorbent detection technology, and it is that one kind is exclusively used in microwell plate extinction
Spend the visible ray photoelectric colorimeter of measure.ELIASA makes absorbance measurement more easy and high speed in enzyme linked immunosorbent detection
Change, specifically refer to Application No. 201010042628.X patent application document《A kind of full-automatic enzyme-linked immunoassay instrument》.
Traditional ELIASA is made up of light source, whirlpool mating plate runner, optical fiber and detection plate etc..Operationally, light source is filtered
8 light beams are divided into by optical fiber after the processing of piece, respectively by 8 micropores on ELISA Plate, had in micropore by integrated enzyme reaction
Solution, light absorbed the photocell on detection reaching board after a part by solution, and photocell converts optical signals to electricity
Signal inputs computer or processor carries out calculating analysis.Under normal circumstances, it is necessary to select filter by rotating filtering piece runner
Mating plate, obtains the light of different wave length.However, this structure is more complicated, space-consuming is larger, is unfavorable for the simplification of structure；Together
Shi Guangyuan power consumption is big, not enough energy-conservation.
The content of the invention
It is convenient, with low cost with simple in construction, miniaturization, manipulation it is an object of the invention to provide a kind of ELIASA
The advantages of.
To achieve these goals, the present invention proposes a kind of ELIASA, including：Display screen, control module, signal acquisition
Module, the first signal plate, secondary signal plate, mounting bracket and light source assembly；Wherein, the control module respectively with the display
Screen, signal acquisition module are connected with light source assembly；Connected between first signal plate and secondary signal plate using signal, it is described
Signal acquisition module can gather all signals on first signal plate simultaneously；The mounting bracket is located at the secondary signal plate
Between light source assembly, the ELISA Plate provided with multiple micropores is arranged in the mounting bracket, the light that the light source assembly is sent
The micropore of beam and the ELISA Plate is corresponded, the measuring samples through in the ELISA Plate micropore, and by the secondary signal
Plate gathers signal.
Further, in described ELIASA, first signal plate and secondary signal plate are provided with multiple micropores
Optical fibre frame, on first signal plate and secondary signal plate the arrangement mode and number of micropore with the ELISA Plate micropore
Arrangement mode is identical with number.
Further, in described ELIASA, the micropore spacing L on first signal plate1Less than the described second letter
Micropore spacing L on number plate2；Micro-pore diameter D on first signal plate1With the micro-pore diameter D on the secondary signal plate2
It is identical.
Further, in described ELIASA, spacing d=3D between the hole of micropore on first signal plate1/2；Its
In, spacing d is the distance between adjacent two micropores center of circle, D between the hole1For the micro-pore diameter on the first signal plate.
Further, in described ELIASA, connected between first signal plate and secondary signal plate using optical fiber,
The optical fiber two ends are separately fixed in the micropore of first signal plate and secondary signal plate and exposed to first signal
The surface of plate and secondary signal plate.
Further, in described ELIASA, the signal acquisition module is a camera module, the camera module pair
The fiber end face of first signal plate is taken pictures, and is obtained through being transmitted after measuring samples to the optical signal of fiber end face, is passed through phase
The control module of connection carries out process signal, and analysis result is presented on a display screen.
Further, in described ELIASA, the optical fiber is arranged in a fibre bundle.
Further, in described ELIASA, the light source assembly includes light emitting diode matrix, uniform light board and optical filtering
Piece；Wherein, the light that the light emitting diode matrix is sent exposes to the bottom of ELISA Plate micropore by the uniform light board and optical filter
Portion.
Further, in described ELIASA, the control module is a circuit module.
Compared with prior art, the beneficial effects are mainly as follows：Measuring samples in ELISA Plate micropore are installed
In mounting bracket, the light beam that light source assembly is sent passes through measuring samples, and secondary signal plate collects corresponding optical signal, and by phase
On the optical signal transmission answered to the first coupled signal plate, signal acquisition is carried out by signal acquisition module is unified.Due to
Signal on one signal plate can be gathered disposably by signal acquisition module simultaneously, so as to avoid the ageing of signal acquisition appearance
Difference, it is to avoid error occurs in testing result, while improving detection efficiency；And with simple in construction, occupy little space, it is with low cost
The advantages of.
Brief description of the drawings
Fig. 1 is the structural representation of ELIASA in one embodiment of the invention；
Fig. 2 is the structural representation of the second optical fibre frame in one embodiment of the invention；
Fig. 3 is the structural representation of the first optical fibre frame in one embodiment of the invention.
Embodiment
The ELIASA of the present invention is described in more detail below in conjunction with schematic diagram, which show the excellent of the present invention
Select embodiment, it should be appreciated that those skilled in the art can change invention described herein, and still realize having for the present invention
Sharp effect.Therefore, description below is appreciated that for the widely known of those skilled in the art, and is not intended as to this
The limitation of invention.
For clarity, not describing whole features of practical embodiments.In the following description, it is not described in detail known function
And structure, because they can make the present invention chaotic due to unnecessary details.It will be understood that opening in any practical embodiments
In hair, it is necessary to make a large amount of implementation details to realize the specific objective of developer, such as according to relevant system or relevant business
Limitation, another embodiment is changed into by one embodiment.Additionally, it should think that this development is probably complicated and expended
Time, but it is only to those skilled in the art routine work.
The present invention is more specifically described by way of example referring to the drawings in the following passage.Will according to following explanation and right
Book is sought, advantages and features of the invention will become apparent from.It should be noted that, accompanying drawing is using very simplified form and using non-
Accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
It is directed to traditional ELIASA space-consuming in the prior art big, complicated, the problems such as cost is high, present invention proposition
A kind of ELIASA simple in construction, has the advantages that miniaturization, manipulation are convenient, with low cost.
In the present embodiment, it is proposed that a kind of ELIASA, including：Display screen, control module, signal acquisition module, first
Signal plate, secondary signal plate, mounting bracket and light source assembly；Wherein, the control module is adopted with the display screen, signal respectively
Collection module carries out signal with light source assembly and is connected, to be controlled to corresponding module；First signal plate and secondary signal plate
Between using signal connection, the signal acquisition module can gather all signals on first signal plate simultaneously；It is described to install
Bracket is located between the secondary signal plate and light source assembly, and the ELISA Plate provided with multiple micropores is arranged on the mounting bracket
On, the micropore of the light beam that the light source assembly is sent and the ELISA Plate is corresponded, through treating in the ELISA Plate micropore
Sample product, and signal is gathered by the secondary signal plate.
Wherein, the display screen is used to show related test results or relevant information；First signal plate and the second letter
Number it is used to carry out the collection of signal；The signal acquisition module is used to carry out corresponding signal acquisition；The mounting bracket is used for
Install is used to install measuring samples in ELISA Plate, ELISA Plate；The light source assembly is used to send corresponding test light.
Specifically, as shown in figure 1, the first signal plate and secondary signal plate are the optical fibre frame provided with multiple micropores.It is described
First signal plate is the first optical fibre frame 4, and shown secondary signal plate is the second optical fibre frame 6.The optical fiber of first optical fibre frame 4 and second
Connected between frame 6 using optical fiber 11, the two ends of optical fiber 11 are separately fixed at the micropore of the first optical fibre frame 4 and the second optical fibre frame 6
Surface that is interior and being exposed to the first optical fibre frame 4 and the second optical fibre frame 6.Optical fiber 11 is managed for convenience, and the optical fiber 11 is arranged on
In one fibre bundle 5.
Under normal circumstances, pore arrangement mode is rectangular arranged in the ELISA Plate used, and specification is 96 holes, and arrangement mode is
12 × 8, its bore dia is usually 7~8mm.In order to correspond collection corresponding signal, first with micropore in ELISA Plate
The arrangement mode of micropore and number need the arrangement mode and number phase with ELISA Plate micropore on the optical fibre frame 6 of optical fibre frame 4 and second
Together.
As shown in Fig. 2 the micro-pore diameter D of the second optical fibre frame 62Determined by plastic optical fiber diameter, adjacent two micropores distance of center circle
From according to being adjusted with a distance from the adjacent holes of 96 hole elisa Plates, fix an optical fiber per hole and be placed in the upper of corresponding ELISA Plate micropore
Side.Need the measuring samples in the ELISA Plate with 96 holes to correspond due to the second optical fibre frame 6 and collect signal, therefore, the second light
The specification of fine frame 6 usually requires consistent with the ELISA Plate specification in 96 holes, and the area that this results in the second optical fibre frame 6 is larger, generally
The length range of second optical fibre frame 6 is 11cm~15cm, and width range is 7cm~10cm.If signal acquisition module is carried out to it
During collection, it is impossible to accomplish and meanwhile disposably gathered whole signals, it is necessary to carry out substep can gather all signals, this
The difference of signal acquisition existence time is had led to, testing result can be caused to there is deviation.
As shown in figure 3, the specification of the first optical fibre frame 4 is also 12 × 8 holes, it needs arrangement mode identical with 96 hole elisa Plates i.e.
Can.Micro-pore diameter D on first optical fibre frame 41With the micro-pore diameter D on the second optical fibre frame 62It is identical.But on the first optical fibre frame 4
Micropore spacing L1Less than the micropore spacing L on the second optical fibre frame 62.Therefore, compared with the second optical fibre frame 6, the face of the first optical fibre frame 4
Product can accomplish smaller, and the length range of the first optical fibre frame 4 is usually 3cm~5cm, and width range is usually 2cm~4cm, energy
Signal acquisition module is carried out once, collection can be while have gathered all signals.Compared to traditional signal acquisition module pair
Second optical fibre frame 6 larger to area carries out the signal acquisition of local substep one by one respectively, and signal acquisition module can be to area
Less first optical fibre frame 4 disposably carries out signal and gathered simultaneously, the plenty of time is saved, further, it is possible to when avoiding signal acquisition
There is error in the signal that the difference of effect property is caused, cause the problem of testing result has inaccurate.
The size of first optical fibre frame 4 is by micro-pore diameter D1Spacing d is determined between plastic optical fiber diameter and hole, spacing between hole
D is the distance in the adjacent holes center of circle, to prevent the optical fiber of the too small adjacent holes of spacing d between hole from producing optical crosstalk problem, needs to set herein
Determine d=3D1/2。
Please continue to refer to Fig. 1, signal acquisition module is a camera module 3, and control module is a circuit module 2.It is described to take the photograph
The fiber end face of 3 pairs of shadow module, first optical fibre frame 4 is taken pictures, and is obtained and is believed through the light transmitted after measuring samples to fiber end face
Number, process signal is carried out by the circuit module 2 being connected, and analysis result is presented on display screen 1.
The light source assembly includes light emitting diode matrix 10, uniform light board 8 and optical filter 9；Wherein, the light emitting diode
The light that array 10 is sent exposes to the bottom of ELISA Plate micropore by the optical filter 9 and uniform light board 8.Light emitting diode matrix 10
On light emitting diode it is corresponding with the hole on ELISA Plate, i.e., a micropore of one light emitting diode correspondence ELISA Plate.
In the present invention ELIASA to implement working method as follows：
First, the light that light emitting diode is sent in light emitting diode matrix 10 filters out irrelevant wave band by optical filter 9
Light, then make bottom that the ELISA Plate micropore equipped with measuring samples is irradiated to after light is uniform by uniform light board 8, ELISA Plate is placed on peace
Fill on bracket 7.
Secondly, after light is by measuring samples, it is coupled into be placed in the optical fiber above micropore and transmits.One micropore correspondence one
Root plastic optical fiber, fibre bundle 5 includes 96 plastic optical fibers, and two ends are separately fixed at the first optical fibre frame 4 and the second optical fibre frame 6
On.
Finally, the fiber end face on 3 pair of first optical fibre frame 4 of photographing module is taken pictures, and is obtained and is transferred to after passing through measuring samples
The optical signal of fiber end face, by the process signal of circuit module 2 being connected, does spectrophotometric analysis according to developing response, draws
Analysis result is simultaneously presented on display screen 1 by light absorption value.
It can be seen that, in operation, as long as the ELISA Plate that will be equipped with measuring samples is placed into mounting bracket, you can analysis
The absorbance of sample, makes immunology judgement, so as to realize enzyme mark detection function.
To sum up, in ELIASA provided in an embodiment of the present invention, the measuring samples in ELISA Plate micropore are arranged on mounting bracket
On frame, the light beam that light source assembly is sent passes through measuring samples, and secondary signal plate collects corresponding optical signal, and corresponding light is believed
Number transmit to the first coupled signal plate, signal acquisition is carried out by signal acquisition module is unified.Due to the first signal plate
On signal can simultaneously disposably be gathered by signal acquisition module so that avoid signal acquisition occur ageing difference, keep away
Exempt from testing result and error occur, while improving detection efficiency；And with simple in construction, occupy little space, the advantages of with low cost.
The preferred embodiments of the present invention are above are only, any restriction effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
1. a kind of ELIASA, it is characterised in that including：Display screen, control module, signal acquisition module, the first signal plate, second
Signal plate, mounting bracket and light source assembly；Wherein, the control module respectively with the display screen, signal acquisition module and light
Source component is connected；Connected between first signal plate and secondary signal plate using signal, the signal acquisition module can be simultaneously
Gather all signals on first signal plate；The mounting bracket is located between the secondary signal plate and light source assembly, if
The ELISA Plate for having multiple micropores is arranged in the mounting bracket, and the light beam that the light source assembly is sent is micro- with the ELISA Plate
Hole is corresponded, the measuring samples through in the ELISA Plate micropore, and gathers signal by the secondary signal plate.
2. ELIASA as claimed in claim 1, it is characterised in that first signal plate and secondary signal plate are provided with many
On the optical fibre frame of individual micropore, first signal plate and secondary signal plate the arrangement mode and number of micropore with the ELISA Plate
The arrangement mode of micropore is identical with number.
3. ELIASA as claimed in claim 2, it is characterised in that the micropore spacing L on first signal plate1Less than described
Micropore spacing L on secondary signal plate2；Micro-pore diameter D on first signal plate1With the micropore on the secondary signal plate
Diameter D2It is identical.
4. ELIASA as claimed in claim 3, it is characterised in that spacing d=3D between the hole of micropore on first signal plate1/
2；Wherein, spacing d is the distance between adjacent two micropores center of circle, D between the hole1For the micro-pore diameter on the first signal plate.
5. ELIASA as claimed in claim 2, it is characterised in that light is used between first signal plate and secondary signal plate
Fibre connection, the optical fiber two ends are separately fixed in the micropore of first signal plate and secondary signal plate and exposed to described the
The surface of one signal plate and secondary signal plate.
6. ELIASA as claimed in claim 5, the signal acquisition module is a camera module, the camera module is to described
The fiber end face of first signal plate is taken pictures, and is obtained and is transmitted after passing through measuring samples to the optical signal of fiber end face, by being connected
Control module carry out process signal, by analysis result present on a display screen.
7. ELIASA as claimed in claim 5, it is characterised in that the optical fiber is arranged in a fibre bundle.
8. ELIASA as claimed in claim 1, it is characterised in that the light source assembly includes light emitting diode matrix, even light
Plate and optical filter；Wherein, the light that the light emitting diode matrix is sent exposes to ELISA Plate by the uniform light board and optical filter
The bottom of micropore.
9. ELIASA as claimed in claim 1, it is characterised in that the control module is a circuit module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710396758.5A CN107102129A (en) | 2017-05-31 | 2017-05-31 | A kind of ELIASA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710396758.5A CN107102129A (en) | 2017-05-31 | 2017-05-31 | A kind of ELIASA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107102129A true CN107102129A (en) | 2017-08-29 |
Family
ID=59659569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710396758.5A Pending CN107102129A (en) | 2017-05-31 | 2017-05-31 | A kind of ELIASA |
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| Country | Link |
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| CN (1) | CN107102129A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110133245A (en) * | 2019-05-24 | 2019-08-16 | 京东方科技集团股份有限公司 | A kind of detection device and detection method, relevant apparatus of enzyme linked immunological test board |
| CN111289434A (en) * | 2020-02-25 | 2020-06-16 | 杭州博日科技有限公司 | Light path position calibration method, calibration tool and fluorescent quantitative detection system |
| CN113358650A (en) * | 2021-06-01 | 2021-09-07 | 浙江大学 | 96-hole microporous plate reader |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1271132A1 (en) * | 2001-06-28 | 2003-01-02 | Fuji Photo Film Co., Ltd. | Method for producing biochemical analysis data and apparatus used therefor |
| CN103969188A (en) * | 2013-02-01 | 2014-08-06 | 苏州英敏基生物技术有限公司 | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor |
| CN204347027U (en) * | 2014-12-12 | 2015-05-20 | 北京智峰博泰生物科技有限公司 | A kind of optical fiber array column immunofluorescence assay device |
-
2017
- 2017-05-31 CN CN201710396758.5A patent/CN107102129A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1271132A1 (en) * | 2001-06-28 | 2003-01-02 | Fuji Photo Film Co., Ltd. | Method for producing biochemical analysis data and apparatus used therefor |
| CN103969188A (en) * | 2013-02-01 | 2014-08-06 | 苏州英敏基生物技术有限公司 | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor |
| CN204347027U (en) * | 2014-12-12 | 2015-05-20 | 北京智峰博泰生物科技有限公司 | A kind of optical fiber array column immunofluorescence assay device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110133245A (en) * | 2019-05-24 | 2019-08-16 | 京东方科技集团股份有限公司 | A kind of detection device and detection method, relevant apparatus of enzyme linked immunological test board |
| CN111289434A (en) * | 2020-02-25 | 2020-06-16 | 杭州博日科技有限公司 | Light path position calibration method, calibration tool and fluorescent quantitative detection system |
| CN113358650A (en) * | 2021-06-01 | 2021-09-07 | 浙江大学 | 96-hole microporous plate reader |
| CN113358650B (en) * | 2021-06-01 | 2024-04-16 | 浙江大学 | 96 hole micropore board reader |
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Application publication date: 20170829 |