CN107094991A - A kind of animal selenium-enriched health care nutrient solution, preparation method and application - Google Patents
A kind of animal selenium-enriched health care nutrient solution, preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of animal selenium-enriched health care nutrient solution, preparation method and application, including Selenium-enriched Yeast Strains seed selection, yeast hydrolyate is obtained, probiotics individual plant ferments, prepared by probiotics mixed liquor, selenium-enriched yeast hydrolysate and probiotics compounding, into probiotics mixed liquor, according to 10~15g/L ratio, the selenium-enriched yeast hydrolysate dry powder of addition；Fresh cultured matrix is added in compounding mixed liquor, potassium sorbate is added into probiotics mixed liquor by mass concentration 0.8~1.0 ‰, stirs, selenium-rich animal health nutrient solution is made.The selenium-rich animal health nutrient solution that the present invention is provided ferments five probiotics respectively, then according to the combination of the ratio of optimization, is acted synergistically between each probiotics strain, with the features such as bacterial strain combination is new, structure is steady, enzyme activity is high, viable count is more.Meanwhile, the hydrolysate of the selenium-enriched yeast of seed selection is added in nutrient solution, organic assembling, preferably plays Organic Selenium and the growth promotion and probiotic effects of probiotics.
Description
Technical field
The present invention relates to a kind of animal feed additive, more particularly to a kind of animal selenium-enriched health care nutrient solution, preparation
Method and application.
Background technology
With the development of China's livestock and poultry breeding industry, scale, intensive livestock and poultry breeding industry emerge rapidly, because of abuse of antibiotics
Producing drug resistance will make the mankind be absorbed in incorrigible bad dream, therefore, the Multiple Classes of Antibiotics similar drug of national regulation 400 must be with hospital
Prescription is bought.But this does not chop the chain that antibiotic poses a health risk off thoroughly yet, because animal abuse of antibiotics threatens
Human health.
At the same time, the theory of animal welfare cultivation is also progressively that numerous culturists, especially scale livestock farming are managed
Person is cognitive and receives.Welfare cultivation refers on the basis of conventinal breeding, builds comfortable breeding environment, use with " it is balanced nutritious-
Body Tiny ecosystem conditioning " is the scientific culture technology of core, rejection ability and non-spy of the enhancing body to harmful intestinal tract microorganism
Specific immunological function, reduces disease, antibiotic usage amount is reduced, so as to obtain the livestock product of high-quality and safety.
Selenium element is the indispensable trace element of growth of animal, is also the important stimulant of animal immune system, is promoted
Antibody tormation, enhancing animal body immune system and anti-infection ability, and the reproductive performance of animal can be improved.In addition, probiotics
After taking in animal body, it can both assist body enhancing to harmful microbe rejection ability in enteron aisle, non-spy can also be strengthened
Specific immunological function, greatly reduces disease, reduces antibiotic dosage, improves economic well-being of workers and staff.Therefore, develop and be rich in selenium element
With the animal health nutrient solution of probiotics, both beneficial functions can effectively be combined, play synergistic function, significantly promote
Enter nutrient balance, improve immunity of organisms.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of animal selenium-enriched health care nutrient solution, prepare
Method and application, meet regulation animal body microecological balance, balanced nutritious, enhancing poultry immunologic function.
The present invention is achieved by the following technical solutions：The present invention comprises the following steps：
(1) Selenium-enriched Yeast Strains seed selection
Candida utili Candida utilis are subjected to the multiple mutagenesis of N~+ implantation, the sub- selenium of enduring high-concentration is obtained
The mutant yeast strains of sour sodium, are named as Candida utilis cas15；
(2) yeast hydrolyate is obtained
By above-mentioned candida utili Candida utilis cas15 inoculations in the fermentation medium, culture obtains selenium-rich
Yeast culture, collects selenium-enriched yeast culture, centrifuges after washing, and richness is obtained after adding mixing enzyme preparation, enzymatic hydrolysis
Yeast hydrolyate containing Organic Selenium and amino acid, is made pale yellow powder shape product after drying, product moisture is less than 8%；
(3) probiotics individual plant ferments
By animal bifidobacteria Bifidobacterium animalis cas13, bacillus subtilis Bacillus
Subtilis cas45, bafillus natto Bacillus natto cas78, streptococcus thermophilus Streptococcus
Thermophiles cas34, Pediococcus acidilactici Pediococcus acidilacticii cas107 this five probiotics bacterial strain
Individually ferment, obtain individual plant probiotics fermention bacterium solution；
(4) prepared by probiotics mixed liquor
By animal bifidobacteria B.animalis cas13, bacillus subtilis B.subtilis cas45, natto gemma
Bacillus B.natto cas78, streptococcus thermophilus S.thermophiles cas34, Pediococcus acidilactici P.acidilacticii
Cas107 is mixed, and probiotics mixed liquor is made, the living bacteria count of obtained probiotics mixed liquor is 1.2 × 109~× 2.1
×109cfu/ml；
(5) selenium-enriched yeast hydrolysate and probiotics compounding
Into the probiotics mixed liquor of step (4), according to 10~15g/L ratio, the Se-enriched yeast water of step (2) is added
Solve thing dry powder；
(6) addition of fresh cultured matrix
Fresh cultured matrix-cane molasses are added in the compounding mixed liquor of step (5)；
(7) potassium sorbate is added in obtained probiotics mixed liquor into step (6) by mass concentration 0.8~1.0 ‰, stirred
Mix uniform, that is, selenium-rich animal health nutrient solution is made.
The step (1) comprises the following steps：
(11) candida utili Candida utilis are using 20~24h of culture, picking single bacterium on YEPD culture plates
In the blake bottle for dropping down onto the fluid nutrient medium containing YEPD, 160~180rpm, 24~36h of concussion and cultivate, bacterium solution centrifuge washing are made
Single cell suspension, makes cell concentration 108~109Individual/mL；
(12) by bacteria suspension even spread into sterile petri dish, air-dry and mycoderm is made；
(13) in N+Ion implanting, 14~18KeV of energy are carried out in implanter；
(14) each bacterial strain does 6 graded doses, each two flat boards of dosage, N+Implantation dosage is respectively 1.5 × 1014、
3.0×1014、4.5×1014、6.0×1014、8.0×1014、1.0×1015ion/cm2, and make vacuum control flat board；
(15) after the completion of mutagenesis, add sterilized water washing thalline and bacteria suspension is made；
(16) bacteria suspension is diluted 104~108The screening and culturing medium containing sodium selenite is coated on again, is sieved by multiple mutagenesis
Choosing, obtains the mutant yeast strains of enduring high-concentration sodium selenite, and its fermentation biomass is under equal conditions than going out bacterium germination
Strain improves more than 1 times, is named as Candida utilis cas15.
In the step (2), the preparation method of selenium-enriched yeast culture is as follows：By candida utili Candida
Utilis cas15 be inoculated with the fermentation medium, 28 DEG C, 160~180r/min, 48~72h of shaken cultivation, obtain selenium-rich ferment
Female culture.
In the step (2), fermentation medium includes malt 10~20g/L of extract, 10~15g/L of peptone, yeast
5~10g/L of cream, 100~180mg/L of sodium selenite.
In the step (2), collection selenium-enriched yeast culture, centrifuge, 8000~10000rpm, centrifugation 5~
10min, washing adds mixing enzyme preparation, and 10~24h of enzymatic hydrolysis obtains the yeast hydrolyate rich in Organic Selenium and amino acid,
160~180 DEG C of spray drying, are made pale yellow powder shape product.
In the step (3), MYP culture mediums include molasses 4.0~6.0%, yeast extract 0.2~0.4%, soybean egg
White peptone 0.1~0.2%, sodium chloride 0.8~1.0%.
In the step (3), it will be inoculated in after each strain fermentation by 1~2% inoculum concentration in MYP fluid nutrient mediums,
180~200rpm, cultivates 24~36h.
In the step (4), by animal bifidobacteria B.animalis cas13, bacillus subtilis B.subtilis
Cas45, bafillus natto B.natto cas78, streptococcus thermophilus S.thermophiles cas34, Pediococcus acidilactici
P.acidilacticii cas107 are according to 1~3：5~8：3~4：1~2：2~4 volume ratio mixing.
A kind of selenium-enriched health care nutrient solution obtained by preparation method preparation using described animal selenium-enriched health care nutrient solution.
A kind of application of selenium-enriched health care nutrient solution in animal feed, the selenium-enriched health care nutrient solution is by weight 1~3 ‰
Added in animal feed.
The present invention has advantages below compared with prior art：The selenium-rich animal health nutrient solution that the present invention is provided is by five plants of benefits
Raw bacterium is fermented respectively, then according to the combination of the ratio of optimization, is acted synergistically between each probiotics strain, with bacterial strain combination it is new,
The features such as structure is steady, enzyme activity is high, viable count is more.Meanwhile, the hydrolysate of the selenium-enriched yeast of seed selection is added in nutrient solution,
Organic assembling, preferably plays Organic Selenium and the growth promotion and probiotic effects of probiotics.
One aspect of the present invention overcomes conventional animal health-care nutrient liquor living bacteria count not up to standard, poor activity, slowly effect etc.
Problem, assists animal body to maintain gut flora balance, suppresses the growth and breeding of pathogen in enteron aisle, and then strengthen the anti-of body
Sick diseases prevention ability, on the other hand, the Organic Selenium being rich in nutrient solution can greatly improve the immunity and resistance of animal body, increase
Strong constitution, remarkably promotes growth of animal.Selenium-enriched yeast hydrolysate and probiotics synergy in the present invention, enhancing livestock and poultry body are supported
Drag, disease is reduced, can partly replace or reduce the use of antibiotic, while cultivation level is improved, reduce antibiosis
The harm that element abuse is caused to environment and human body, promotes the cultivation of nonreactive breeding ecological in the popularization of China.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementations
Example.
Embodiment 1
The concrete component of the selenium-rich animal health nutrient solution of the present embodiment is as follows：
The preparation method of the present embodiment is as follows：
(1) Selenium-enriched Yeast Strains seed selection
Candida utili Candida utilis on YEPD culture plates using 24h is cultivated, and picking single bacterium, which is dropped down onto, to be contained
In the 250ml triangular flasks of 100ml YEPD fluid nutrient mediums, list is made in 180rpm, concussion and cultivate 36h, bacterium solution centrifuge washing 3 times
Cell suspension, makes cell concentration 108~109Individual/mL.Take 0.1mL bacteria suspension even spreads into sterile petri dish, be placed in super
Net workbench, which is air-dried, is made mycoderm.In N+Ion implanting, energy 18KeV are carried out in implanter.Each bacterial strain does 6 gradient agent
Amount, each two flat boards of dosage.N+Implantation dosage is respectively 1.5 × 1014、3.0×1014、4.5.0×1014、6.0×1014、
8.0×1014、1.0×1015ion/cm2, and make vacuum control flat board.After the completion of mutagenesis, 1ml sterilized water washing thalline systems are added
Into bacteria suspension.Bacteria suspension is diluted 1108The screening and culturing medium containing sodium selenite is coated on again, using 3,3'- diaminobenzidines
Chloride process determines the content of yeast strain selenium.By multiple mutagenesis screening, enduring high-concentration sodium selenite (180mg/L) is obtained
Mutant yeast strains, and its fermentation biomass under equal conditions improves nearly 1.5 times compared with starting strain, is named as
Candida utilis cas15。
(2) yeast hydrolyate is obtained
Above-mentioned candida utili Candida utilis cas15 are seeded in 500mL fermentation medium, 28 DEG C,
160rpm, shaken cultivation 48h, obtain selenium-enriched yeast culture.Fermentation medium components are constituted：Malt extract 10g/L, egg
White peptone 10g/L, yeast extract 5g/L, sodium selenite 100mg/L；
Selenium-enriched yeast culture is collected, centrifuge, 8000rpm centrifuges 5min, and washing adds in mass ratio 1:1 mixes
The wall breaking enzyme of conjunction, the mixing enzyme preparation of papain, enzymatic hydrolysis 10h obtain the yeast water rich in Organic Selenium and amino acid
Solve thing.160 DEG C of spray drying, are made pale yellow powder shape product, and product moisture is less than 8%.
(3) probiotics individual plant ferments
Animal bifidobacteria Bifidobacterium animalis cas13, bacillus subtilis Bacillus
Subtilis cas45, bafillus natto Bacillus natto cas78, streptococcus thermophilus Streptococcus
The five probiotics bacterial strains such as thermophiles cas34, Pediococcus acidilactici Pediococcus acidilacticii cas107
Individually ferment, obtain individual plant probiotics fermention bacterium solution.All probiotics fermention culture mediums use MYP culture medium (molasses
4.0%th, yeast extract 0.2%, soy peptone 0.1%, sodium chloride 0.8%) culture；1% inoculum concentration is inoculated in MYP liquid
In culture medium, 180rpm cultivates 24h.
(4) prepared by probiotics mixed liquor
After above-mentioned each single strain fermentation ends, by animal bifidobacteria B.animalis cas13, bacillus subtilis
B.subtilis cas45, bafillus natto B.natto cas78, streptococcus thermophilus S.thermophiles cas34, breast
Sour piece coccus P.acidilacticii cas107 are according to 1:5:3:1:2 volume ratio mixing, is made probiotics mixed liquor.It is made
The living bacteria count of the probiotics mixed liquor obtained is 1.2 × 109cfu/ml。
(5) selenium-enriched yeast hydrolysate-probiotics compounding
Into probiotics mixed liquor, according to 10g/L ratio, selenium-enriched yeast hydrolysate dry powder is added.
(6) addition of fresh cultured matrix
Fresh cultured matrix-cane molasses are added into selenium-enriched yeast hydrolysate-probiotics mixed liquor by mass concentration 3%
(containing sugar more than 50%, brix (Bx) more than 85%).
(7) potassium sorbate (purity is added in obtained probiotics mixed liquor into step (6) by mass concentration 0.8 ‰
More than 99%), stir (100rpm stirs 1h), that is, selenium-rich animal health nutrient solution is made.
Selenium-rich animal health nutrient solution made from the present embodiment is tested in certain pig breeding farm and records as follows：
Test site：The glad auspicious pig breeding farm in Quzhou City of Zhejiang Province Qujiang District
Test material：Weanling pig, selenium-rich animal health nutrient solution prepared by embodiment 1.
Experimental design：One test group, a control group, every group of piglet 100 are set.
Control group：Piglet is raised according to normal rearing conditions, and feeding amount, antibiotic usage are with other normal piglets.
Test group：Piglet is raised according to normal rearing conditions, and feeding amount is with other normal piglets, according to 1 ‰ in feed
Ratio mixes the animal health nutrient solution applied and prepared in the present embodiment, without using antibiotic.
Result of the test：Using the piglet of animal health nutrient solution manufactured in the present embodiment, phenomenon occurs for yellowish-white clever substantially
Disappear, compared with control group, yellowish-white clever incidence reduces in 85.7%, and breeding process and do not use any antibiotic, significantly
Reduce antibiotic usage cost, pollution of the reduction abuse of antibiotics to environment.Meanwhile, using the animal of the preparation of the present embodiment
The pig speed of growth of health-care nutrient liquor feeding can shift to an earlier date 5 days and deliver for sale, greatly reduce the feed of culturist apparently higher than control group
Cost.Therefore, on the one hand the animal health nutrient solution of the present embodiment can substantially reduce utilization of the antibiotic in breeding process,
The pollution that abuse of antibiotics is caused is reduced, it is also possible to culturist's feeding cost be substantially reduced, with good economic benefit
And ecological benefits.
Embodiment 2
The selenium-rich animal health nutrient solution concrete component of the present embodiment is as follows：
The preparation method of the present embodiment is as follows：
(1) Selenium-enriched Yeast Strains seed selection, it is same as Example 1.
(2) yeast hydrolyate is obtained
Above-mentioned candida utili Candida utilis cas15 are seeded in 500mL fermentation medium, 28 DEG C,
170rpm, shaken cultivation 60h, obtain selenium-enriched yeast culture.Fermentation medium components are constituted：Malt extract 15g/L, egg
White peptone 12g/L, yeast extract 8g/L, sodium selenite 150mg/L.
Selenium-enriched yeast culture is collected, centrifuge, 9000rpm centrifuges 10min, and washing adds in mass ratio 1:1
The wall breaking enzyme of mixing, the mixing enzyme preparation of papain, enzymatic hydrolysis 20h obtain the yeast rich in Organic Selenium and amino acid
Hydrolysate.180 DEG C of spray drying, are made pale yellow powder shape product, and product moisture is less than 7%.
(3) probiotics individual plant ferments
Animal bifidobacteria Bifidobacterium animalis cas13, bacillus subtilis Bacillus
Subtilis cas45, bafillus natto Bacillus natto cas78, streptococcus thermophilus Streptococcus
The five probiotics bacterial strains such as thermophiles cas34, Pediococcus acidilactici Pediococcus acidilacticii cas107
Individually ferment, obtain individual plant probiotics fermention bacterium solution.All probiotics fermention culture mediums use MYP culture medium (molasses
5.0%th, yeast extract 0.3%, soy peptone 0.15%, sodium chloride 0.9%) culture；1.5% inoculum concentration is inoculated in MYP
In fluid nutrient medium, 190rpm cultivates 30h.
(4) prepared by probiotics mixed liquor
After above-mentioned each single strain fermentation ends, by animal bifidobacteria B.animalis cas13, bacillus subtilis
B.subtilis cas45, bafillus natto B.natto cas78, streptococcus thermophilus S.thermophiles cas34, breast
Sour piece coccus P.acidilacticii cas107 are according to 2:6:3.5:1.5:3 volume ratio mixing, is made probiotics mixed liquor.
The living bacteria count of obtained probiotics mixed liquor is 1.6 × 109cfu/ml。
(5) selenium-enriched yeast hydrolysate-probiotics compounding
Into probiotics mixed liquor, according to 12g/L ratio, selenium-enriched yeast hydrolysate dry powder is added.
(6) addition of fresh cultured matrix：
Fresh cultured matrix-cane molasses are added into selenium-enriched yeast hydrolysate-probiotics mixed liquor by mass concentration 4%
(containing sugar more than 50%, brix (Bx) more than 85%).
(7) potassium sorbate (purity is added in obtained probiotics mixed liquor into step (6) by mass concentration 0.9 ‰
More than 99%), stir (100rpm stirs 2h), that is, selenium-rich animal health nutrient solution is made.
Selenium-rich animal health nutrient solution made from the present embodiment is tested in certain pig breeding farm and records as follows：
Test site：The glad auspicious pig breeding farm in Quzhou City of Zhejiang Province Qujiang District
Test material：Weanling pig, selenium-rich animal health nutrient solution manufactured in the present embodiment.
Experimental design：Control group and test group, every group of piglet 100 are set.
Control group：Piglet is raised according to normal rearing conditions, and feeding amount, antibiotic usage are with other normal piglets.
Test group：Piglet is raised according to normal rearing conditions, and feeding amount is with other normal piglets, according to 2 ‰ in feed
Ratio mixes the animal health nutrient solution applied and prepared in the present embodiment, without using antibiotic.
Result of the test：Using the piglet of animal health nutrient solution manufactured in the present embodiment, phenomenon occurs for yellowish-white clever substantially
Disappear, compared with control group, yellowish-white clever incidence reduces in 90%, and breeding process and do not use any antibiotic, drops significantly
Low antibiotic usage cost, pollution of the reduction abuse of antibiotics to environment.Meanwhile, using animal health manufactured in the present embodiment
The pig speed of growth of nutrient solution feeding can shift to an earlier date 10 days and deliver for sale apparently higher than control group, greatly reduce the feed of culturist into
This.Therefore, on the one hand the animal health nutrient solution of the present embodiment can substantially reduce utilization of the antibiotic in breeding process, subtract
The pollution that few abuse of antibiotics is caused, it is also possible to substantially reduce culturist's feeding cost, with good economic benefit and
Ecological benefits.
Embodiment 3
The selenium-rich animal health nutrient solution concrete component of the present embodiment is as follows：
The preparation method of the present embodiment is as follows：
(1) Selenium-enriched Yeast Strains seed selection, it is same as Example 1.
(2) yeast hydrolyate is obtained
Above-mentioned candida utili Candida utilis cas15 are seeded in 500mL fermentation medium, 28 DEG C,
180rpm, shaken cultivation 72h, obtain selenium-enriched yeast culture.Fermentation medium components are constituted：Malt extract 20g/L, egg
White peptone 15g/L, yeast extract 10g/L, sodium selenite 180mg/L.
Selenium-enriched yeast culture is collected, centrifuge, 10000rpm centrifuges 10min, and washing adds in mass ratio 1:1
The wall breaking enzyme of mixing, the mixing enzyme preparation of papain, enzymatic hydrolysis 24h obtain the yeast rich in Organic Selenium and amino acid
Hydrolysate.170 DEG C of spray drying, are made pale yellow powder shape product, and product moisture is less than 7%.
(3) probiotics individual plant ferments
Animal bifidobacteria Bifidobacterium animalis cas13, bacillus subtilis Bacillus
Subtilis cas45, bafillus natto Bacillus natto cas78, streptococcus thermophilus Streptococcus
The five probiotics bacterial strains such as thermophiles cas34, Pediococcus acidilactici Pediococcus acidilacticii cas107
Individually ferment, obtain individual plant probiotics fermention bacterium solution.All probiotics fermention culture mediums use MYP culture medium (molasses
6.0%th, yeast extract 0.4%, soy peptone 0.2%, sodium chloride 1.0%) culture；2% inoculum concentration is inoculated in MYP liquid
In culture medium, 200rpm cultivates 36h.
(4) prepared by probiotics mixed liquor
After above-mentioned each single strain fermentation ends, by animal bifidobacteria B.animalis cas13, bacillus subtilis
B.subtilis cas45, bafillus natto B.natto cas78, streptococcus thermophilus S.thermophiles cas34, breast
Sour piece coccus P.acidilacticii cas107 are according to 3:8:4:2:4 volume ratio mixing, is made probiotics mixed liquor.It is made
The living bacteria count of the probiotics mixed liquor obtained is 2.1 × 109cfu/ml。
(5) selenium-enriched yeast hydrolysate-probiotics compounding
Into probiotics mixed liquor, according to 15g/L ratio, selenium-enriched yeast hydrolysate dry powder is added.
(6) addition of fresh cultured matrix
Fresh cultured matrix-cane molasses are added into selenium-enriched yeast hydrolysate-probiotics mixed liquor by mass concentration 5%
(containing sugar more than 50%, brix (Bx) more than 85%).
(7) potassium sorbate (purity is added in obtained probiotics mixed liquor into step (6) by mass concentration 1.0 ‰
More than 99%), stir (100rpm stirs 2h), that is, selenium-rich animal health nutrient solution is made.
Selenium-rich animal health nutrient solution made from the present embodiment is tested in certain pig breeding farm and records as follows：
Test site：The glad auspicious pig breeding farm in Quzhou City of Zhejiang Province Qujiang District
Test material：Weanling pig, selenium-rich animal health nutrient solution prepared by embodiment 3.
Experimental design：Control group and test group, every group of piglet 100 are set.
Control group：Piglet is raised according to normal rearing conditions, and feeding amount, antibiotic usage are with other normal piglets.
Test group：Piglet is raised according to normal rearing conditions, and feeding amount is with other normal piglets, according to 3 ‰ in feed
Ratio mixes the animal health nutrient solution applied and prepared in embodiment 3, without using antibiotic.
Result of the test：Using animal health nutrient solution manufactured in the present embodiment, piglet yellow-white clever occurs phenomenon and substantially disappeared
Lose, compared with control group, yellowish-white clever incidence reduces in 80%, and breeding process and do not use any antibiotic, substantially reduces
Antibiotic usage cost, pollution of the reduction abuse of antibiotics to environment.Meanwhile, sought using animal health manufactured in the present embodiment
The pig speed of growth of nutrient solution feeding can shift to an earlier date 8 days and deliver for sale, greatly reduce the feed cost of culturist apparently higher than control group.
Therefore, on the one hand the animal health nutrient solution of the present embodiment can substantially reduce utilization of the antibiotic in breeding process, reduce
The pollution that abuse of antibiotics is caused, it is also possible to culturist's feeding cost be substantially reduced, with good economic benefit and life
State benefit.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of preparation method of animal selenium-enriched health care nutrient solution, it is characterised in that comprise the following steps：
(1) Selenium-enriched Yeast Strains seed selection
Candida utili Candida utilis are subjected to the multiple mutagenesis of N~+ implantation, enduring high-concentration sodium selenite is obtained
Mutant yeast strains, be named as Candida utilis cas15；
(2) yeast hydrolyate is obtained
By above-mentioned candida utili Candida utilis cas15 inoculations in the fermentation medium, culture obtains Se-enriched yeast
Culture, collects selenium-enriched yeast culture, centrifuges after washing, and adding to obtain being rich in after mixing enzyme preparation, enzymatic hydrolysis has
The yeast hydrolyate of machine selenium and amino acid, is made pale yellow powder shape product after drying, product moisture is less than 8%；
(3) probiotics individual plant ferments
By animal bifidobacteria Bifidobacterium animalis cas13, bacillus subtilis Bacillus
Subtilis cas45, bafillus natto Bacillus natto cas78, streptococcus thermophilus Streptococcus
Thermophiles cas34, Pediococcus acidilactici Pediococcus acidilacticii cas107 this five probiotics bacterial strain
Individually ferment, obtain individual plant probiotics fermention bacterium solution；
(4) prepared by probiotics mixed liquor
By animal bifidobacteria B.animalis cas13, bacillus subtilis B.subtilis cas45, bafillus natto
B.natto cas78, streptococcus thermophilus S.thermophiles cas34, Pediococcus acidilactici P.acidilacticii cas107
Mixing, is made probiotics mixed liquor, the living bacteria count of obtained probiotics mixed liquor is 1.2 × 109~× 2.1 ×
109cfu/ml；
(5) selenium-enriched yeast hydrolysate and probiotics compounding
Into the probiotics mixed liquor of step (4), according to 10~15g/L ratio, the selenium-enriched yeast hydrolysate of step (2) is added
Dry powder；
(6) addition of fresh cultured matrix
Fresh cultured matrix-cane molasses are added in the compounding mixed liquor of step (5)；
(7) potassium sorbate is added in obtained probiotics mixed liquor into step (6) by mass concentration 0.8~1.0 ‰, stirring is equal
It is even, that is, selenium-rich animal health nutrient solution is made.
2. a kind of preparation method of animal selenium-enriched health care nutrient solution according to claim 1, it is characterised in that the step
(1) comprise the following steps：
(11) candida utili Candida utilis are dropped down onto using 20~24h, picking single bacterium is cultivated on YEPD culture plates
In the blake bottle of the fluid nutrient medium containing YEPD, 160~180rpm, 24~36h of concussion and cultivate, bacterium solution centrifuge washing are made slender
Born of the same parents' suspension, makes cell concentration 108~109Individual/mL；
(12) by bacteria suspension even spread into sterile petri dish, air-dry and mycoderm is made；
(13) in N+Ion implanting, 14~18KeV of energy are carried out in implanter；
(14) each bacterial strain does 6 graded doses, each two flat boards of dosage, N+Implantation dosage is respectively 1.5 × 1014、3.0×
1014、4.5×1014、6.0×1014、8.0×1014、1.0×1015ion/cm2, and make vacuum control flat board；
(15) after the completion of mutagenesis, add sterilized water washing thalline and bacteria suspension is made；
(16) bacteria suspension is diluted 104~108The screening and culturing medium containing sodium selenite is coated on again, by multiple mutagenesis screening, is obtained
The mutant yeast strains of enduring high-concentration sodium selenite are obtained, and its fermentation biomass is under equal conditions improved than starting strain
More than 1 times, it is named as Candida utilis cas15.
3. a kind of preparation method of animal selenium-enriched health care nutrient solution according to claim 1, it is characterised in that the step
(2) in, the preparation method of selenium-enriched yeast culture is as follows：Candida utili Candida utilis cas15 are seeded in hair
In ferment culture medium, 28 DEG C, 160~180r/min, 48~72h of shaken cultivation, obtain selenium-enriched yeast culture.
4. a kind of preparation method of animal selenium-enriched health care nutrient solution according to claim 1, it is characterised in that the step
(2) in, fermentation medium includes malt 10~20g/L of extract, 10~15g/L of peptone, 5~10g/L of yeast extract, selenous acid
100~180mg/L of sodium.
5. a kind of preparation method of animal selenium-enriched health care nutrient solution according to claim 1, it is characterised in that the step
(2) in, selenium-enriched yeast culture is collected, centrifuge, 8000~10000rpm centrifuges 5~10min, and washing adds mixing
Enzyme preparation, 10~24h of enzymatic hydrolysis obtains the yeast hydrolyate rich in Organic Selenium and amino acid, and 160~180 DEG C are spray-dried,
Pale yellow powder shape product is made.
6. a kind of preparation method of animal selenium-enriched health care nutrient solution according to claim 1, it is characterised in that the step
(3) in, MYP culture mediums include molasses 4.0~6.0%, yeast extract 0.2~0.4%, soy peptone 0.1~0.2%,
Sodium chloride 0.8~1.0%.
7. a kind of preparation method of animal selenium-enriched health care nutrient solution according to claim 1, it is characterised in that the step
(3) it in, will be inoculated in MYP fluid nutrient mediums, 180~200rpm, cultivate by 1~2% inoculum concentration after each strain fermentation
24~36h.
8. a kind of preparation method of animal selenium-enriched health care nutrient solution according to claim 1, it is characterised in that the step
(4) in, by animal bifidobacteria B.animalis cas13, bacillus subtilis B.subtilis cas45, natto gemma bar
Bacterium B.natto cas78, streptococcus thermophilus S.thermophiles cas34, Pediococcus acidilactici P.acidilacticii
Cas107 is according to 1~3：5~8：3~4：1~2：2~4 volume ratio mixing.
9. a kind of preparation method of animal selenium-enriched health care nutrient solution using as described in any one of claim 1~8 prepares gained
Selenium-enriched health care nutrient solution.
10. application of a kind of selenium-enriched health care nutrient solution in animal feed as claimed in claim 9, the selenium-enriched health care nutrition
Liquid is added in animal feed by weight 1~3 ‰.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110402862A (en) * | 2019-08-28 | 2019-11-05 | 湖北省农业科学院农业经济技术研究所(湖北省农业科学院农业规划设计研究所) | One kind is rich in organic selenium cray feed and preparation method thereof and cultural method |
| CN111631317A (en) * | 2019-03-01 | 2020-09-08 | 张德珍 | Animal nutrient solution containing nano-selenium compound bacterium enzyme |
| CN113519708A (en) * | 2020-04-20 | 2021-10-22 | 安琪酵母股份有限公司 | Yeast composition for improving weaning feed intake of piglets and reducing diarrhea rate, and preparation and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103343118A (en) * | 2013-06-22 | 2013-10-09 | 湖南角山米业有限责任公司 | Biological selenium product applied to organic selenium-rich agriculture and preparation method thereof |
| CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
| CN106187331A (en) * | 2016-07-13 | 2016-12-07 | 中国科学院合肥物质科学研究院 | One utilizes fowl and animal excrement to prepare complex microorganism ecology bacterial manure and method thereof |
-
2017
- 2017-04-26 CN CN201710281364.5A patent/CN107094991A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103343118A (en) * | 2013-06-22 | 2013-10-09 | 湖南角山米业有限责任公司 | Biological selenium product applied to organic selenium-rich agriculture and preparation method thereof |
| CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
| CN106187331A (en) * | 2016-07-13 | 2016-12-07 | 中国科学院合肥物质科学研究院 | One utilizes fowl and animal excrement to prepare complex microorganism ecology bacterial manure and method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631317A (en) * | 2019-03-01 | 2020-09-08 | 张德珍 | Animal nutrient solution containing nano-selenium compound bacterium enzyme |
| CN110402862A (en) * | 2019-08-28 | 2019-11-05 | 湖北省农业科学院农业经济技术研究所(湖北省农业科学院农业规划设计研究所) | One kind is rich in organic selenium cray feed and preparation method thereof and cultural method |
| CN113519708A (en) * | 2020-04-20 | 2021-10-22 | 安琪酵母股份有限公司 | Yeast composition for improving weaning feed intake of piglets and reducing diarrhea rate, and preparation and application thereof |
| CN113519708B (en) * | 2020-04-20 | 2024-01-02 | 安琪酵母股份有限公司 | Yeast composition for improving weaning feed intake of piglets and reducing diarrhea rate as well as preparation and application thereof |
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