CN107090409A - Cell culture apparatus - Google Patents
Cell culture apparatus Download PDFInfo
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- CN107090409A CN107090409A CN201710531542.5A CN201710531542A CN107090409A CN 107090409 A CN107090409 A CN 107090409A CN 201710531542 A CN201710531542 A CN 201710531542A CN 107090409 A CN107090409 A CN 107090409A
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- cell
- gas
- cell culture
- orifice plate
- culture apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to field of cell culture, more particularly to a kind of cell culture apparatus, including cell culture chamber, the cell observation cover on cell culture chamber, the cell culture assembly in cell culture room, and a cell gas control assembly.Cell culture chamber, including a base and the supporting frame that is connected with the base；Cell observation cover is fixedly connected with the upper end of supporting frame, and is suitable to the observation port that microscope is probed provided with one at the top of the cell observation cover；Cell culture assembly, on base, and including the first body and the second body, is equipped with a magnet, i.e. the first body and the second body by the mutual attracted formation culture dish of magnet in the first body and the second body；Cell gas control assembly, through being arranged on the wall of supporting frame side, including gas detection cell, the gas introduction unit that is connected with gas detection cell, and the control unit being electrically connected with gas detection cell and gas introduction unit.
Description
Technical field
The present invention relates to field of cell culture, more particularly to a kind of cell culture apparatus.
Background technology
In biotechnology, cell culture is an essential process.Cell culture is usually to put cell
Enter blake bottle, then be put into progress in incubator.During cell culture blake bottle can be taken out from incubator and microscope is put into
Under to observe the growing state of cell.Under normal circumstances because the temperature of incubator is higher than room temperature, the vapor in blake bottle
Water smoke can be condensed into after quenching on bottle top.Water smoke can cause light diffusing reflection so that microscope imaging is unclear.
Having cultivated the vessel after cell needs cleaning, but general vessel cleaning up is inconvenient, it is easy to which cleaning is not done
Only；And because cell culture needs certain gaseous environment, it is necessary to which rationally control gas concentration is also the key of cell culture.
The content of the invention
It is an object of the invention to provide a kind of novel cell culture device, it is easy to control culture thin when solving cell culture
The gas composition of born of the same parents' environment, and reduction Tissue Culture Dish clean the technical problem of difficulty.
In order to solve the above-mentioned technical problem, it is the invention provides technical scheme：A kind of cell culture apparatus, including cell
Culturing room, the cell observation cover on the cell culture chamber, the cell culture assembly in the cell culture room, with
And a cell gas control assembly；Wherein cell culture chamber, including a base and the supporting frame that is connected with the base；Cell is seen
The upper end that cover is examined with support frame as described above body is fixedly connected, and is suitable to the sight that microscope is probed provided with one at the top of the cell observation cover
Survey mouth；Cell culture assembly, on the base, and including the first body and the second body, first body and second
A magnet is equipped with body, i.e., described first body and the second body are by the mutual attracted formation culture dish of the magnet；Institute
State and some culture cells are provided with culture dish；And cell gas control assembly, through being arranged on support frame as described above body side
On wall, including gas detection cell, the gas introduction unit that is connected with the gas detection cell, and detected with the gas
The control unit that unit and gas introduction unit are electrically connected with.
Further, the top of the cell observation cover by located at arranged on left and right sides can around center line overturn right baffle-plate and
Right shell body is constituted, and the relative one side of left and right baffle is provided with symmetrical semicircle opening；I.e. left and right baffle is altogether
Just described observation port is formed；The observation port is additionally provided with a shelves lid for being used to close observation port.
Further, described supporting frame is to be opened respectively on hollow column structure, the arranged on left and right sides face of the supporting frame
Provided with a circular hole, the circular hole is respectively connected with the operation gloves inside support frame as described above body；And support frame as described above body
Upper trailing flank offers the through hole of the installation cell gas control assembly.
Further, the culture cell includes one side attracted with second body on first body
The first orifice plate, and one side attracted with first body on second body the second orifice plate；It is described
First orifice plate and the second orifice plate are semicircular structure, i.e., described first orifice plate and the second orifice plate are due to first body and
Two bodies mutual attracted and form the circular culture cell.
Further, one is equipped with the both sides end face of the openend of described semicircle first orifice plate is used to seal the training
Support the sealed connecting component of cell；The both sides end face of the openend of semicircular second orifice plate is provided with and is tightly connected with described
The projection suitable for being caught in the sealed connecting component that part position is correspondingly arranged.
Further, the sealed connecting component is I-shaped, and the I-shaped sealed connecting component includes the U-shaped recess of both sides,
Side recess is inlayed with the end face of the first orifice plate openend to be connected；Some jacks are provided with the U-shaped recess of opposite side；And institute
Projection is stated provided with some contact pins being connected with some jacks.
Further, the gas detection cell is described thin on the madial wall of support frame as described above body, including for detecting
In born of the same parents culturing room object gas and with described control unit be electrically connected with sensor group.
Further, the gas introduction unit located at support frame as described above body lateral wall on, including with described control unit
The gas inducting device of electric connection, and the object gas storage tank group being connected with the gas inducting device.
Further, it is additionally provided between the gas inducting device and the object gas storage tank group and the gas inducting device
The gas solenoid valve of electric connection.
Further, the object gas is oxygen and carbon dioxide.Beneficial effects of the present invention are：Using cell observation cover
The environment of observation of constant temperature can be provided for cell culture, it is to avoid fog is produced, and convenient operation and observation.And in support frame
Operation gloves are set on body, and required angle can be adjusted by facilitating during micro- sem observation.
Further, described cell culture assembly by the first body and the second body adhesive moreover, being further cultured for cell
Afterwards, conveniently take cleaning apart, make cleaning more thorough.
Further, using cell gas control assembly, the gas concentration in cell culture room can be detected, reasonably
Control the gas content needed for cell culture.
Brief description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the overall structure diagram of the novel cell culture device of the present invention；
Fig. 2 is the structural representation of the cell observation cover of the present invention；
Fig. 3 is the structural representation of the cell gas control assembly of the present invention；
Fig. 4 is the structural representation of the cell culture assembly of the present invention；
Fig. 5 is the first orifice plate of the present invention and the structural representation of the second orifice plate.
In figure：Cell culture chamber 1, cell observation cover 2, cell culture assembly 3, cell gas control assembly 4, base 5, branch
Support body 6, observation port 7, the first body 8, the second body 9, culture dish 10, culture cell 11, right baffle-plate 12, right shell body 13, half
Circular open 14, circular hole 15, operation gloves 16, shelves lid 17, the first orifice plate 18, the second orifice plate 19, sealed connecting component 20, projection
21st, jack 22, contact pin 23, gas detection cell 24, gas introduction unit 25, gas storage tanks group 26, control unit 27.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
In the description of the invention, it is to be understood that term " upper end ", " lower end ", " relative ", " left side ",
" right side ", " top ", " bottom ", " interior ", the orientation of the instruction such as " outer " or position relationship be based on orientation shown in the drawings or position
Relation, is for only for ease of the description present invention and simplifies description, rather than indicate or imply that the device or element of meaning must have
There is specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.In retouching for the present invention
In stating, unless otherwise indicated, " some " are meant that two or more.
Embodiment 1：
As shown in Figures 1 to 5, the invention provides a kind of cell culture apparatus, including cell culture chamber 1, located at the cell
Cell observation cover 2 in culturing room 1, the cell culture assembly 3 in the cell culture chamber 1, and a cell gas control
Component 4 processed；Wherein cell culture chamber 1, including a base 5 and the supporting frame 6 that is connected with the base 5；Cell observation cover 2 and institute
The upper end for stating supporting frame 6 is fixedly connected, and the top of the cell observation cover 2 is suitable to the observation port 7 that microscope is probed provided with one.
Cell observation cover 2 is used to provide the environment of observation of constant temperature for cell culture, it is to avoid fog is produced, and convenient operation is with seeing
Examine.It is tightly connected between the cell observation cover 2 and supporting frame 6.
Optionally, cell observation cover 2 is with supporting the junction of support body 6 provided with a circle sealing ring, and the sealing ring can use soft
Property silica gel material is made.
Optionally, the base 5 can be using such as, but not limited to cup dolly 5 or direction base 5 etc..
Specifically, cell culture assembly 3 is on the base 5, and including the first body 8 and the second body 9, described the
A magnet is equipped with one body 8 and the second body 9, i.e., the described body 9 of first body 8 and second is mutually attracting by the magnet
Conjunction forms culture dish 10；Some culture cells 11 are provided with the culture dish 10；And
Cell gas control assembly 4, through being arranged on the side wall of support frame as described above body 6, including gas detection cell 24 and institute
State gas detection cell 24 connection gas introduction unit 25, and with the gas detection cell 24 and gas introduction unit 25
The control unit 27 of electric connection.
As shown in Fig. 2 the top of the cell observation cover 2 is by the left gear that can be overturn around center line located at arranged on left and right sides
Plate 12 and right shell body 13 are constituted, and the relative one side of left and right baffle is provided with symmetrical semicircle opening 14；It is i.e. left and right
Baffle plate just forms described observation port 7 altogether；The observation port 7 is additionally provided with a shelves lid 17 for being used to close observation port 7.It is described
Shelves lid 17 is used to close the observation port.
Optionally, the shelves lid 17 is made using such as, but not limited to silica gel material.
Observation port 7 is passed through suitable for microscopical eyepiece.And the shelves lid 17 is during the cell culture do not observed
Observation port 7 is blocked, the culture space of cell culture is formed.
Described supporting frame 6 is to offer one respectively on hollow column structure, the arranged on left and right sides face of the supporting frame
Circular hole 15, the circular hole 15 is respectively connected with the operation gloves 16 inside support frame as described above body 6；And support frame as described above body
Trailing flank offers the through hole of the installation cell gas control assembly 4 on 6.
When being observed, the shelves lid 17 of observation port 7 is taken away, eyepiece is observed through observation port 7, passes through manipulator
Set 16 contain the angular adjustment of liquid vessel 8 and microscope ocular.
Wherein optional, supporting frame 6 can select such as, but not limited to cylinder, the four prisms cylinder etc. coordinated with base 5.
As shown in figure 4, culture cell 11 is included attracted with second body 9 one on first body 8
First orifice plate 18 of side, and one side attracted with first body 8 on second body 9 the second hole
Plate 19；The orifice plate 19 of first orifice plate 18 and second is semicircular structure, i.e., the described orifice plate 19 of first orifice plate 18 and second by
The circular culture cell 11 is formed in the mutual attracted of the body 9 of the first body 8 and second.
Optionally, culture cell 11 can set three groups or more.
As shown in figure 5, one is equipped with the end face of the openend of described semicircle first orifice plate 18 is used to seal described
Cultivate the sealed connecting component 20 of cell 11；The end face of the openend of semicircular second orifice plate 19 is provided with and the sealing
The projection 21 suitable for being caught in the sealed connecting component 20 that the position of connector 20 is correspondingly arranged.
Wherein, the sealed connecting component 20 is laid on the openend of the first orifice plate 18 and the second orifice plate 19 with projection 21 respectively
Whole end face on, it is ensured that the first orifice plate 18 and the second orifice plate 19, which are inhaled, to be combined together rear adhesive face and all seals, the training formed
Cell 11 is supported without leak dead angle.
It is preferred that, the sealed connecting component 20 is I-shaped, and the I-shaped sealed connecting component 20 includes the U-shaped recessed of both sides
Mouthful, side recess is inlayed with the end face of the openend of the first orifice plate 18 to be connected；Some jacks are provided with the U-shaped recess of opposite side
22；And the projection 21 is provided with some contact pins 23 being connected with some jacks 22.
The size of wherein described projection 21 is identical with the interior pocket dimension of the I-shaped sealed connecting component 20.Pass through contact pin
In 23 insertion jacks 22, projection 21 is set to be caught in completely in sealed connecting component 20.
Optionally, the sealed connecting component 20 is made using silica gel material, and the flexibility of sealed connecting component 20 is good, can protect
Positive confinement when demonstrate,proving the first orifice plate 18 and the second 19 adhesive of orifice plate, will not leak.
The gas detection cell 24 is located on the madial wall of support frame as described above body 6, including for detecting the cell training
Support room 1 in object gas and with described control unit 27 be electrically connected with sensor group.It is wherein optional, the sensor group
Including oxygen concentration sensor and gas concentration lwevel sensor.Pass through oxygen concentration sensor and gas concentration lwevel sensor
The oxygen and gas concentration lwevel in cell culture chamber 1 are detected, by gas concentration information transmission to control unit 27.
The gas introduction unit 25 is electric on the lateral wall of support frame as described above body 6, including with described control unit 27
Property connection gas inducting device, and the object gas storage tank group 26 being connected with the gas inducting device.When control unit 27
When judging inadequate oxygen concentration or inadequate gas concentration lwevel, control unit 27 opens gas inducting device and corresponding gas
Storage tank, the insufflation gas into cell culture chamber 1.And optional, described gas storage tanks group 26 corresponds to oxygen storage
Tank and carbon dioxide holding tank.
Wherein, control unit 27 is also disposed on the lateral wall for supporting support body, and control unit 27 includes one and is used to show
Show gas concentration and the operation interface for being easy to experimenter's arrange parameter in cell culture chamber 1.
It is additionally provided between the gas inducting device and the object gas storage tank group 26 electrical with the gas inducting device
The gas solenoid valve of connection.By the opening and closing of magnetic valve, the opening and closing of gas storage tanks is controlled.Each gas storage tanks are led with gas
Enter and be designed with a gas solenoid valve between device.
Optionally, the object gas is oxygen and carbon dioxide.
If gas is different needed for cell, required sensor can also be changed by gas according to needed for cell, and gas is deposited
Gas in reservoir.Concentration parameter is set by control unit 27.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of cell culture apparatus, it is characterised in that the cell including cell culture chamber, on the cell culture chamber is seen
Examine cover, the cell culture assembly in the cell culture room, and a cell gas control assembly；Wherein cell culture
Room, including a base and the supporting frame that is connected with the base；Cell observation cover is fixedly connected with the upper end of support frame as described above body,
And it is suitable to the observation port that microscope is probed provided with one at the top of the cell observation cover；Cell culture assembly, located at the base
On, and including the first body and the second body, a magnet is equipped with first body and the second body, i.e., described first device
Body and the second body are by the mutual attracted formation culture dish of the magnet；Some culture cells are provided with the culture dish；With
And cell gas control assembly, through being arranged on the wall of support frame as described above body side, including gas detection cell, with the gas
The gas introduction unit of detection unit connection, and the control being electrically connected with the gas detection cell and gas introduction unit
Unit.
2. cell culture apparatus according to claim 1, it is characterised in that the top of the cell observation cover by located at
The right baffle-plate that can be overturn around center line and right shell body of arranged on left and right sides are constituted, and the relative one side of left and right baffle is provided with
Symmetrical semicircle opening；I.e. left and right baffle just forms described observation port altogether；The observation port, which is additionally provided with one, to be used to seal
Close the shelves lid of observation port.
3. cell culture apparatus according to claim 1, it is characterised in that described supporting frame is hollow cylinder knot
Structure；A circular hole is offered on the arranged on left and right sides face of the supporting frame respectively, the circular hole is respectively connected with one and is located at support frame as described above
The operation gloves in internal portion；And trailing flank offers the logical of the installation cell gas control assembly on support frame as described above body
Hole.
4. cell culture apparatus according to claim 1, it is characterised in that the culture cell includes being located at described first
First orifice plate of the one side attracted with second body on body, and with described first on second body
Second orifice plate of the attracted one side of body；First orifice plate and the second orifice plate are semicircular structure, i.e., and described first
Orifice plate and the second orifice plate form the circular culture cell due to the mutual attracted of first body and the second body.
5. cell culture apparatus according to claim 4, it is characterised in that the openend of described semicircle first orifice plate
Both sides end face on be equipped with one and be used to seal the sealed connecting component of the culture cell；Semicircular second orifice plate is opened
The both sides end face at mouthful end is provided with is caught in the convex of the sealed connecting component with being suitable to of being correspondingly arranged of the sealed connecting component position
Block.
6. cell culture apparatus according to claim 5, it is characterised in that the sealed connecting component be it is I-shaped, it is described
I-shaped sealed connecting component includes the U-shaped recess of both sides, and side recess is inlayed with the end face of the first orifice plate openend to be connected；
Some jacks are provided with the U-shaped recess of opposite side；And the projection is provided with some contact pins being connected with some jacks.
7. cell culture apparatus according to claim 1, it is characterised in that the gas detection cell is located at the support
On the madial wall of support body, including for detecting object gas in the cell culture room and be electrically connected with described control unit
Sensor group.
8. cell culture apparatus according to claim 7, it is characterised in that the gas introduction unit is located at the support
On the lateral wall of support body, including the gas inducting device being electrically connected with described control unit, and connect with the gas inducting device
The object gas storage tank group connect.
9. cell culture apparatus according to claim 8, it is characterised in that the gas inducting device and the object gas
The gas solenoid valve being electrically connected with the gas inducting device is additionally provided between storage tank group.
10. cell culture apparatus according to claim 9, it is characterised in that the object gas is oxygen and titanium dioxide
Carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710531542.5A CN107090409A (en) | 2017-07-03 | 2017-07-03 | Cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710531542.5A CN107090409A (en) | 2017-07-03 | 2017-07-03 | Cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107090409A true CN107090409A (en) | 2017-08-25 |
Family
ID=59640951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710531542.5A Withdrawn CN107090409A (en) | 2017-07-03 | 2017-07-03 | Cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107090409A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500453A (en) * | 2020-04-30 | 2020-08-07 | 龙岩学院 | Sealed cultivation device for biological cell cultivation and use method thereof |
| CN113755331A (en) * | 2021-10-19 | 2021-12-07 | 中国人民解放军西部战区总医院 | Cell culture case of simulation plateau environment |
-
2017
- 2017-07-03 CN CN201710531542.5A patent/CN107090409A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500453A (en) * | 2020-04-30 | 2020-08-07 | 龙岩学院 | Sealed cultivation device for biological cell cultivation and use method thereof |
| CN113755331A (en) * | 2021-10-19 | 2021-12-07 | 中国人民解放军西部战区总医院 | Cell culture case of simulation plateau environment |
| CN113755331B (en) * | 2021-10-19 | 2023-10-24 | 中国人民解放军西部战区总医院 | Cell incubator of simulation altitude environment |
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Application publication date: 20170825 |