CN107080006A - A kind of preparation method of high theophylline Pu'er cooked tea - Google Patents
A kind of preparation method of high theophylline Pu'er cooked tea Download PDFInfo
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- CN107080006A CN107080006A CN201710474233.9A CN201710474233A CN107080006A CN 107080006 A CN107080006 A CN 107080006A CN 201710474233 A CN201710474233 A CN 201710474233A CN 107080006 A CN107080006 A CN 107080006A
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- fermentation
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- theophylline
- aspergillus sydowii
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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Abstract
The invention belongs to tea processing technical field, a kind of preparation method of high theophylline Pu'er cooked tea is disclosed, is comprised the following steps：The pure culture of aspergillus sydowii strain；The expansion culture of aspergillus sydowii strain；The inoculating microbes pile-fermentation of aspergillus sydowii strain；The post-processing of pile-fermentation.The present invention will produce the effective strain of theophylline --- and aspergillus sydowii is manually seeded in big-leaf species in yunnan solar dried green tea after expanding culture through pure culture, and pile-fermentation is carried out in its natural state, and high theophylline Pu'er cooked tea is obtained after spreading the processes such as ageing；The inventive method is simple to operation, and obtained high theophylline Pu'er cooked tea Determination of Theophylline Concentration reaches as high as more than 3% 2% or so, in the prevention and treatment of respiratory disease, and has certain health-care efficacy in terms of PFT improvement.
Description
Technical field
The invention belongs to tea processing technical field, more particularly to a kind of preparation method of high theophylline Pu'er cooked tea.
Background technology
Purine base is main alkaloid in tealeaves, mainly including caffeine (1,3,7- trimethyl xanthine), theobromine
(3,7- dimethyl xanthines), theophylline (1,3- dimethyl xanthines) etc..In general tealeaves and tea tree breed, caffeine content
It is higher, it is 2%~5%；Theobromine contents are taken second place, and are 0.2%~0.4%；Determination of Theophylline Concentration is minimum, only 0.008%~
0.2%.Three kinds of purine bases have different health-care efficacies and physiologically active.Caffeine and theobromine mainly have excited maincenter god
Through, reduction cholesterol, cardiac stimulant, diuresis the effects such as.Theophylline can relax bronchial smooth muscle, with good antiasthmatic effect；To fertilizer
Maxicell and basophilic granulocyte release histamine have inhibitory action, with good anti-inflammatory；In acute and chronic treating asthma
In be widely used.Black tea is the after fermentation teas under Chinese distinctive microorganism is dominated.Pu'er cooked tea as black tea one kind,
It is that, using big-leaf species in yunnan solar dried green tea as raw material, being formed through the comprehensive function such as microorganism, enzyme, damp and hot, oxidation has exclusive quality
The tea type of feature.Pile-fermentation (after fermentation, solid state fermentation) is the critical process of Pu'er cooked tea quality responses.
Under the main trend that present tealeaves develops, broad masses of the people increasingly pay attention to the health-care efficacy of tealeaves.It is modern
Medical science thinks that theophylline has good health-care efficacy in the treatment, PFT improvement and the preventing and treating of breathing problem of asthma.
In summary, the problem of prior art is present be：
Prior art, is not also inoculated with solar dried green tea by inoculating microbe and carries out Pu'er cooked tea pile-fermentation, processing
Go out a kind of prevention respiratory disease, improve the high theophylline Pu'er cooked tea of PFT.
The present invention obtains mitogenetic rich in aspergillus sydowii by pure culture-expansion culture of pharmaceutical microorganism-aspergillus sydowii
The strain of spore, and strain is seeded to the pile-fermentation that Pu'er cooked tea is carried out in solar dried green tea according to a certain percentage, obtain
Pu'er cooked tea Determination of Theophylline Concentration is between 1.9755% to 3.0758%.Obtained by high inoculation group (3.0% inoculum concentration) pile-fermentation
Pu'er cooked tea Determination of Theophylline Concentration is up to more than 3%, has certain booster action to the treatment of acute and chronic asthma, and can improve Human Lung
Function, there is good preventive effect to respiratory disease.
The content of the invention
The problem of existing for prior art, the invention provides a kind of preparation method of high theophylline Pu'er cooked tea.
The present invention is to obtain high by processes such as aspergillus sydowii-pure culture-expansion culture-inoculating microbes-nature pile fermentations
Theophylline Pu'er cooked tea.A kind of preparation method of high theophylline Pu'er cooked tea, the preparation method of the high theophylline Pu'er cooked tea, including with
Lower step：
Step one, the pure culture of aspergillus sydowii strain:600 grams of big-leaf species in yunnan solar dried green teas are weighed, addition moisture makes tea
Leaf water content is 35%；Average mark puts into 10 conical flasks；After 121 DEG C of 15min that sterilize, every bottle of inoculation 5mL 108CFU/mL
Aspergillus sydowii bacteria suspension；It is placed in 35 DEG C, the constant temperature humidity chamber of 85% humidity and cultivates；After culture 14 days, take out, sterile
Dry in the air dry to foot under state；
Step 2, the expansion culture of aspergillus sydowii strain：12000 grams of big-leaf species in yunnan solar dried green teas are weighed, moisture is added
It is 35%~38% to make water content of tea；After 121 DEG C of high pressure-temperature sterilizings, room temperature is cooled to, with poly- song more obtained by step one
Mould species are mixed according to mass ratio, are carried out strain and are expanded culture；
Step 3, the inoculation pile-fermentation of aspergillus sydowii strain：1000 kilograms of big-leaf species in yunnan solar dried green teas are weighed, successively
Moisture is added, and according to the 1.5%-3% gained aspergillus sydowii strain of ratio inoculation step two, pile fermentation hair is carried out after being well mixed
Ferment；Whole pile-fermentation continues 35 days, and turning, even heap were carried out every 5-7 days, and adds amount of water in good time, to maintain micro- life
The metabolism of thing；
Step 4, the subsequent treatment of pile-fermentation：Screening that pile-fermentation is spontaneously dried after terminating -- storage ageing --
Pick and pick；Complete the preparation of Pu'er cooked tea.
Further, in described pure culture, expansion culture, inoculation pile-fermentation, the strain applied is aspergillus sydowii
(Aspergillus sydowii)。
Further, in step one, the aspergillus sydowii strain obtained by described aspergillus sydowii strain pure culture contains aspergillus sydowii
Quantity is in 1.0*1010CFU/g, miscellaneous bacteria bacterium colony is not more than 5%.
Further, in step 2, the inoculum concentration that described aspergillus sydowii expands aspergillus sydowii in culture is 5%, expands training
The strain quantity containing aspergillus sydowii for supporting gained is more than 1.0*108CFU/g, miscellaneous bacteria bacterium colony is not more than 5%.
Further, in step 3, in described aspergillus sydowii inoculation pile-fermentation, the inoculum concentration of aspergillus sydowii strain is
1.5%-3.0%, the initial tidewater amount of tealeaves of pile-fermentation heap is 37%~48%, a height of 70 centimetres~80 centimetres of pile fermentation heap,
Pile fermentation heap temperature is at 35 DEG C~55 DEG C.
Another object of the present invention is to provide a kind of preparation method using above-mentioned high theophylline Pu'er cooked tea to prepare tool
There is the health care Pu'er cooked tea for preventing and treating asthma.
Another object of the present invention is to provide a kind of preparation method using above-mentioned high theophylline Pu'er cooked tea to prepare tool
There is the health care Pu'er cooked tea for improving PFT.
Advantages of the present invention and good effect are：
The present invention utilizes the effective strain that one plant of production theophylline is filtered out in Pu'er tea pile fermentation, is through molecular biology identification
Aspergillus sydowii (Aspergillus sydowii).Caffeine can be converted into theophylline by the bacterial strain by demethylation, be high tea
The production of alkali Pu'er cooked tea provides good quality strain.Research has shown that, aspergillus sydowii can secrete produce it is a variety of with active anticancer
Indole alkaloid and miscellaneous oxygen anthrone, are important Medical Microbiology, medicine and Related product production in obtain tentatively should
With.The present invention carries out Pu'er cooked tea pile-fermentation using the inoculating microbes of aspergillus sydowii, processes high theophylline Pu'er cooked tea, mesh
Before yet there are no relevant report.The present invention passes through the works such as pure culture-expansion culture-inoculating microbes-nature pile fermentation of aspergillus sydowii
The high theophylline Pu'er cooked tea Determination of Theophylline Concentration that sequence is obtained reaches as high as more than 3%, in the prevention of respiratory disease 2% or so
And treatment, and have certain health-care efficacy in terms of PFT improvement.
Brief description of the drawings
Fig. 1 is the preparation method flow chart of high theophylline Pu'er cooked tea provided in an embodiment of the present invention.
Colonial morphology figure of Fig. 2 aspergillus sydowii bacterial strains provided in an embodiment of the present invention on culture medium.
In figure：A is PDA fronts, and b is the PDA back sides, and c is Cha Shi fronts, and d is the Cha Shi back sides.
The morphological feature figure of Fig. 3 aspergillus sydowii bacterial strains provided in an embodiment of the present invention under an electron microscope.
In figure：A is conidium (× 400), and b is conidial head (× 200), and c is double-deck conidial fructification：Metulae, bottle
Obstruct (× 200), d is conidiophore (× 200).
Fig. 4 is different vaccination group Determination of Theophylline Concentration variation diagram in pile-fermentation provided in an embodiment of the present invention.
Fig. 5 is application bacterial strain systematic evolution tree schematic diagram provided in an embodiment of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle to the present invention is described in detail below in conjunction with the accompanying drawings.
As shown in figure 1, the preparation method of high theophylline Pu'er cooked tea provided in an embodiment of the present invention, comprises the following steps：
S101:The pure culture of aspergillus sydowii strain:600 grams of big-leaf species in yunnan solar dried green teas are weighed, the appropriate moisture of addition makes
Water content of tea is 35%, and average mark puts into 10 conical flasks.After 121 DEG C of 15min that sterilize, every bottle of inoculation 5mL 108CFU/
ML aspergillus sydowii bacteria suspensions, are placed in 35 DEG C, the constant temperature humidity chamber of 85% humidity and cultivate；After culture 14 days, take out, in nothing
Dry in the air dry to foot under bacterium state.
S102:The expansion culture of aspergillus sydowii strain：12000 grams of big-leaf species in yunnan solar dried green teas are weighed, suitable quantity of water is added
Dividing makes water content of tea be 35%~38%.After high pressure-temperature sterilizing, room temperature is cooled to, with aspergillus sydowii strain obtained by S101
It is well mixed according to 5% mass ratio, carry out strain and expand culture.
S103:The inoculation pile-fermentation of aspergillus sydowii strain：1000 kilograms of big-leaf species in yunnan solar dried green teas are weighed, are added successively
Plus amount of water, and aspergillus sydowii strain obtained by S102 is inoculated with according to 1.5%-3% ratio, carry out pile fermentation hair after being well mixed
Ferment.Whole pile-fermentation continues 35 days or so, and turning, even heap are carried out at regular intervals, and adds amount of water in good time, to tie up
Hold the metabolism of microorganism.
S104:The subsequent treatment of pile-fermentation：Pile-fermentation is spontaneously dried after terminating -- storage ageing --, and screening is picked
The manufacturing procedure such as pick, to complete the preparation of Pu'er cooked tea.
The strain that the processes such as pure culture of the present invention, expansion culture, inoculation pile-fermentation are applied is poly- more bent
Mould (Aspergillus sydowii).
Aspergillus sydowii strain quantity containing aspergillus sydowii obtained by aspergillus sydowii strain pure culture of the present invention is in 1.0*
1010CFU/g, miscellaneous bacteria bacterium colony must not exceed 5%.
The inoculum concentration that aspergillus sydowii of the present invention expands aspergillus sydowii in culture is 5%, expands the bacterium obtained by culture
Quantity containing aspergillus sydowii is planted in 1.0*108More than CFU/g, miscellaneous bacteria bacterium colony is no more than 5%.
In aspergillus sydowii inoculation pile-fermentation of the present invention, the inoculum concentration of aspergillus sydowii strain is wet more than 1.5%
The initial tidewater amount (water content) of tealeaves of heap fermentation heap is 37%~48%, and pile fermentation heap height is in 70 centimetres~80 centimetres, pile fermentation heap
Temperature is at 35 DEG C~55 DEG C.
The application principle of the present invention is further described with reference to specific embodiment.
Material employed in the embodiment of the present invention, other used material, reagents etc. unless otherwise specified, can be from
Commercial sources are obtained.
1. material
It is original to meet the Yunnan large-leaf seed solar dried green tea of GB/T 22111-2008 Pu'er tea national geography famous special product requirements
Material.It is using ITS1-5.8S-ITS2rRNA sequences (516bp) the SEQ ID NO1 of bacterial strain：
CTGCCTCCGGGCGCCCACCTCCCACCCGTGAATACCTAACACTGTTGCTTCGGCGGGGAACCCCCTCGGGGGCGAGC
CGCCGGGGACTACTGAACTTCATGCCTGAGAGTGATGCAGTCTGAGTCTGAATATAAAATCAGTCAAAACTTTCAAC
AATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAACTGCGATAAGTAATGTGAATTGCAGAATTCAGTGA
ATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGCATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCA
TCAAGCCCGGCTTGTGTGTTGGGTCGTCGTCCCCCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGTGTCCGG
TCCTCGAGCGTATGGGGCTTTGTCACCCGCTCGACTAGGGCCGGCCGGGCGCCAGCCGACGTCTCCAACCATTTTTC
TTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAAA。
Retrieval is compared by online Blast and combines Fig. 5 systematic evolution trees, belongs to aspergillus using bacterial strain, with typical strain
The Phylogenetics of Aspergillus sydowiiNRRL 250 are nearer, and similitude is 99.8%.
2. test apparatus
JR-1003 types electronic balance (Shanghai Xin Nuo instrument plants)；(Jintan City's environmental protection of HH-S28s type digital displays thermostat water bath
Instrument plant)；DFZ-O1 type electric vacunm drying casees (Hengfeng Medical Instruments Co., Ltd., Huangsih City)；JH-752 types UV, visible light point
Luminometer (Shanghai essence tech equipment Co., Ltd)；(U.S.'s Agilent is public for Agilent1200 types high performance liquid chromatograph
Department)；Low temperature refrigerator (Japanese SANYO companies)；FZ-102 type microphyte samples pulverizer (light Medical Instruments factory of Beijing)；
Labonce-150TH constant temperature humidity chambers (Beijing Lan Beishi constant temperature technologies Co., Ltd)；SFG type solid-state fermenters (Zhenjiang
Jiang Gong bioengineering equipment company).
Below in conjunction with the accompanying drawings and specific embodiment to the present invention application principle be further described.
The preparation method of high theophylline Pu'er cooked tea provided in an embodiment of the present invention, including：
The pure culture of step (1) application bacterial strain：
It is accurate to weigh 600 grams of big-leaf species in yunnan solar dried green teas, amount of water is added, it is 35% or so to make water content of tea,
Average mark puts into 10 conical flasks.After 121 DEG C of sterilizing 15min, every bottle of inoculation 5mL108CFU/mL aspergillus sydowii bacteria suspensions.It is placed in
35 DEG C, the constant temperature humidity chamber of 85% humidity is interior to be cultivated.Culture 14 days, after bacterium colony is ripe, takes out；Enter in its natural state
Row is spread to being completely dried；After the completion of to be dried, collect in sterile closed container, the expansion culture for strain.According to GB
4789.15-2010 national food safety standard《Assay method in food microbiological examination mould and yeast counts》To pure
Cultivate obtained strain and carry out micro organism quantity measure.As a result as shown in following table (table 1), aspergillus sydowii quantity is 2.38 in strain
×109~4.30 × 1010CFU/g, average value is 1.25 × 1010CFU/g, miscellaneous bacteria bacterium colony account for aspergillus sydowii bacterium colony 0.41%~
7.69%, average out to 3.59%.Miscellaneous bacteria according to《Fungal identification handbook》、《Chinese fungi will》、《Experiment on Microbiology handbook》Tentatively
It is judged as Penicillium notatum, saccharomycete, aspergillus niger etc..
Aspergillus sydowii and miscellaneous bacteria clump count under the different dilution factors of table 1
Note：Miscellaneous bacteria clump count in bracket.
The expansion culture of step (2) application strain：
It is accurate to weigh 12000 grams of solar dried green teas, amount of water is added, makes water content of tea between 35%-40%.121
DEG C sterilizing 15min, be cooled to after room temperature, the strain obtained with step (1) is mixed according to 5% (w/w).Sterilized
The solid state fermentation of strain is carried out in fermentation tank.The air after aseptic process is passed through in solid ferment process, moisture is no longer added.Gu
Aspergillus sydowii and other miscellaneous bacteria number changes are shown in Table 2 in state fermentation.When fermenting 3 days, tea base starts aspergillus sydowii occur
Bacterium colony, and start amount reproduction.When fermenting 5 days, aspergillus sydowii quantity reaches 7*107CFU/g.Aspergillus sydowii number when fermenting 9 days
Amount has reached 1.2*108CFU/g.Prolonged solid state fermentation can increase the risk of other living contaminantses.Fermentation 9 days is treated, is gathered many
Aspergillus quantity (CFU/g) reaches 108Terminate fermentation after the order of magnitude, spontaneously dried.Should in solid state fermentation dries tealeaves
Prevent the pollution of the red miscellaneous bacteria such as mould of black mould, mould, white mould.Aspergillus sydowii quantity reaches 1*108CFU/g, miscellaneous bacteria quantity should be controlled
System is below 5%.It can be used for high theophylline after expanding the natural drying of the strain rich in aspergillus sydowii that culture (solid state fermentation) is obtained
The natural pile-fermentation of Pu'er cooked tea.
Aspergillus sydowii growth pattern in the solid state fermentation of table 2 and drying process
The inoculation pile-fermentation of step (3) aspergillus sydowii strain produces high theophylline Pu'er cooked tea：
To be obtained by step (2) by matrix solid state fermentation of big-leaf species in yunnan solar dried green tea rich in aspergillus sydowii strain
Strain is mixed according to certain inoculum concentration with big-leaf species in yunnan solar dried green tea, and adds appropriate drinking water, makes tealeaves
Initial tidewater amount (water content) is maintained between 37%-48%.Solar dried green tea used in pile fermentation is more than 1000kg, pile fermentation stack height
At 70 centimetres to 80 centimetres, the burlap after upper covering steam sterilizing processing.High inoculum concentration fermentation group (3% inoculum concentration) is set respectively
With low inoculum concentration fermentation group (1.5% inoculum concentration), and photograph is used as using the natural pile-fermentation without inoculation.During the fermentation every five
Its turning once, and is deblocked in first time or second of turning.Whole fermentation process is 35 days or so.Pile-fermentation knot
-- storage ageing -- is spontaneously dried after beam, and screening, which is picked, picks；Complete the preparation of Pu'er cooked tea.Determination of Theophylline Concentration takes efficient liquid phase
Method (HPLC).
Different vaccination fermentation group Determination of Theophylline Concentration is as shown in Figure 4.In without inoculation nature pile-fermentation, Determination of Theophylline Concentration is without significantly
Degree increase, is typically maintained in less than 0.2%.In inoculation pile-fermentation, Determination of Theophylline Concentration is sharply increased；During fermentation ends, low
In inoculation group and high inoculation group, Determination of Theophylline Concentration is respectively up to 1.9755 ± 0.162% and 3.0758 ± 0.155%, compared with raw material
35 times and 56 times are respectively increased.GINA (Global Initiative forAsthma, GINA) hair in 2006
Theophylline is classified as asthma additional therapeutic agent by the guide of table, and recommendation gives load for daily 4-6mg/kgb.w..With human body
Normal type 60kg is counted, and 0.24-0.32 grams of theophylline can be taken daily.According to average 8 grams of gauge of drinking tea, if Determination of Theophylline Concentration in tealeaves
Up to 3% or so, daily theophylline dose is 0.24 gram, is the minimum value that theophylline recommends dose, can reach treatment or prevents urgency
Property and chronic asthma disease purpose, and PFT etc. can be improved.Therefore, high inoculum concentration (3% inoculum concentration) is suitable for high theophylline
The production and application of Pu'er cooked tea.
Using the different pile fermentation stage tea samples of high inoculation group (3% inoculum concentration) and sample tea as material, respectively to water extraction, tea
Polyphenol, catechin, Pigments (theaflavin, thearubigin, theabrownin), the physical and chemical composition such as free amino acid is analyzed.Water logging
Go out thing measure：Take GB/T 8305-2013.Tea Polyphenols is determined：Tartaric acid iron colorimetric method for determining is taken with reference to GB/T 8313-
2008.Tealeaves total catechin content is determined：Take Vanillin colorimetric method.Tea Pigment assay in tealeaves：TFs, TRs and
TBs assay takes system analysis method.Free amino acid total amount is determined：Ninhydrin colorimetry is taken with reference to GB/T8314-
2013.The main physico-chemical analysis result of different fermentations stage and sample tea is as shown in table 3.Pu'er cooked tea is inoculated with pile-fermentation process
In with traditional pile-fermentation, main chemical compositions variation tendency is consistent, be presented that water extraction is reduced, Tea Polyphenols is reduced, tea
The variation tendency that brown cellulose content significantly increases.《GB/T22111-2008 national geography famous special product Pu'er teas》It is required that Pu'er is ripe
Tea (loose tea) water extraction content >=28%, polyphenol content≤25%.High inoculation group pile-fermentation sample tea water extraction contains
Measure as 36.3%, polyphenol content is 13.6%, is met《Geography symbol product Pu'er tea》Basic demand.
The change of main physical and chemical index during the high inoculation group pile fermentation of table 3
Colonial morphology figure of Fig. 2 aspergillus sydowii bacterial strains provided in an embodiment of the present invention on culture medium.
In figure：A is PDA fronts, and b is the PDA back sides, and c is Cha Shi fronts, and d is the Cha Shi back sides.
The morphological feature figure of Fig. 3 aspergillus sydowii bacterial strains provided in an embodiment of the present invention under an electron microscope.
In figure：A is conidium (× 400), and b is conidial head (× 200), and c is double-deck conidial fructification：Metulae, bottle
Obstruct (× 200), d is conidiophore (× 200).
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
<110>Applicant's title Yunnan Prov Agriculture University Zhou Binxing
<120>A kind of preparation method of high theophylline Pu'er cooked tea
<160> 1
<210> 1
<211>516
<212> RNA
<213>Artificial sequence
<400>RNA sequence
CTGCCTCCGGGCGCCCACCTCCCACCCGTGAATACCTAACACTGTTGCTTCGGCGGGGAACCCCCTCGGGGGC
GAGCCGCCGGGGACTACTGAACTTCATGCCTGAGAGTGATGCAGTCTGAGTCTGAATATAAAATCAGTCAAAACTTT
CAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAACTGCGATAAGTAATGTGAATTGCAGAATTCA
GTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGCATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTG
CCCATCAAGCCCGGCTTGTGTGTTGGGTCGTCGTCCCCCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGTGT
CCGGTCCTCGAGCGTATGGGGCTTTGTCACCCGCTCGACTAGGGCCGGCCGGGCGCCAGCCGACGTCTCCAACCATT
TTTCTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAAA。
Claims (7)
1. a kind of preparation method of high theophylline Pu'er cooked tea, it is characterised in that the preparation method of the high theophylline Pu'er cooked tea, bag
Include following steps：
Step one, the pure culture of aspergillus sydowii strain:600 grams of big-leaf species in yunnan solar dried green teas are weighed, addition moisture contains tealeaves
Water is 35%；Average mark puts into 10 conical flasks；After 121 DEG C of 15min that sterilize, every bottle of inoculation 5mL 108CFU/mL is poly- more
Aspergillus suspension；It is placed in 35 DEG C, the constant temperature humidity chamber of 85% humidity and cultivates；After culture 14 days, take out, in germ-free condition
Under dry in the air dry to foot；
Step 2, the expansion culture of aspergillus sydowii strain：12000 grams of big-leaf species in yunnan solar dried green teas are weighed, addition moisture makes tea
Leaf water content is 35%~38%；High pressure-temperature sterilizing after, be cooled to room temperature, with step one gained aspergillus sydowii strain according to
Certain mass ratio is well mixed, and is carried out strain and is expanded culture；
Step 3, the inoculation pile-fermentation of aspergillus sydowii strain：1000 kilograms of big-leaf species in yunnan solar dried green teas are weighed, are added successively
Moisture, and the gained aspergillus sydowii strain of inoculation step two according to a certain percentage, pile-fermentation is carried out after being well mixed；Whole pile fermentation
Fermentation lasts 35 days, carry out turning, even heap at regular intervals, and add amount of water in good time, to maintain the metabolism of microorganism；
Step 4, the subsequent treatment of pile-fermentation：Pile-fermentation is spontaneously dried after terminating -- storage ageing --, and screening, which is picked, picks；
Complete the preparation of Pu'er cooked tea.
2. the preparation method of high theophylline Pu'er cooked tea as claimed in claim 1, it is characterised in that described pure culture, expand
In culture, inoculation pile-fermentation, the strain applied is aspergillus sydowii (Aspergillus sydowii).
3. the preparation method of high theophylline Pu'er cooked tea as claimed in claim 1, it is characterised in that in step one, described is poly-
Aspergillus sydowii strain quantity containing aspergillus sydowii obtained by many aspergillus strain pure cultures is in 1.0*1010CFU/g, miscellaneous bacteria bacterium colony is not more than
5%.
4. the preparation method of high theophylline Pu'er cooked tea as claimed in claim 1, it is characterised in that in step 2, described is poly-
The inoculum concentration of aspergillus sydowii in mass ratio is 5% in many aspergillus expansion cultures, expands the strain number containing aspergillus sydowii obtained by culture
Amount is more than 1.0*108CFU/g, miscellaneous bacteria bacterium colony is not more than 5%.
5. the preparation method of high theophylline Pu'er cooked tea as claimed in claim 1, it is characterised in that step 3 kind, described is poly-
In many aspergillus inoculation pile-fermentations, the inoculum concentration of aspergillus sydowii strain is more than 1.5%, the initial tidewater amount of tealeaves of pile-fermentation heap
For 37%~48%, a height of 70 centimetres~80 centimetres of pile fermentation heap, pile fermentation heap temperature is at 35 DEG C~55 DEG C.
6. a kind of preparation method of the high theophylline Pu'er cooked tea described in utilization claim 1, prepares to have and prevents and treats asthma health care
The Pu'er cooked tea of effect.
7. a kind of preparation method of the high theophylline Pu'er cooked tea described in utilization claim 1, preparing to have improves PFT health care
The Pu'er cooked tea of effect.
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| CN110250296A (en) * | 2019-08-01 | 2019-09-20 | 云南臻字号茶业有限责任公司 | A kind of plateau ancient tree tea Pu'er cooked tea processing technology |
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