CN107064287B - Germ content device for fast detecting and detection method in liquid - Google Patents
Germ content device for fast detecting and detection method in liquid Download PDFInfo
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- CN107064287B CN107064287B CN201710370874.XA CN201710370874A CN107064287B CN 107064287 B CN107064287 B CN 107064287B CN 201710370874 A CN201710370874 A CN 201710370874A CN 107064287 B CN107064287 B CN 107064287B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/72—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables
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Abstract
The present invention relates to germ content device for fast detecting and detection method in liquid, belongs to magnetostriction detection equipment technology field, solves the technical problem of germ content quick detection in constant prepare liquid, solution is：Electrode upper surface is installed with discharge opeing velar plate, lower surface is provided with handle, upper end is provided with limited block, and monitor station is arranged between upper limit position block and lower position block, and monitor station upper surface is provided with sensor, passes through spring connection through hole cover plate above monitor station；Detection trench bottom is provided with leakage fluid dram；Washing pipe is provided with below detect tank, guide thimble is extended in detect tank through washing pipe, and motor moves up and down in guide thimble, monitor station base is provided with washing pipe, monitor station base is in Helmholtz coil.The present invention adjusts the frequency and amplitude of magnetostrictor by adjusting constant current size and a-c cycle, by the combination of material physical characteristic in itself and Biological Knowledge, quickly realizes the quantitative detection of germ in testing liquid.
Description
Technical field
The invention belongs to the invention belongs to magnetostriction detection equipment technology field, more particularly to germ content is fast in liquid
Speed detector and detection method.
Background technology
Food security is one of public health problem that the whole world is paid close attention to jointly, is pacified concerning body health of people and life
Entirely.Shown according to the investigation result about food security cause, the food-safety problem that food origin disease triggers is that the whole world is current
Primary food-safety problem.The World Health Organization estimates that the annual food origin disease case that occurs in the whole world is up to 40-60 hundred million, and
In ascendant trend year by year, wherein about 3,000,000 less than 5 years old death of child.In China, there is more than 200,000,000 person-times to suffer from food every year on average
Source property disease, i.e., averagely just there is 1 people to suffer from food origin disease in 6.5 people.According to the statistics report of CDC
Announcement shows, in food origin disease, 95% above is caused by pathogenic bacteria, and its incidence of disease occupies the forefront of various diseases total incidence.
An effective approach for preventing to be entered human foods chain by the food of pathogen contamination is exactly to be monitored at initial control point.
In this context, highly sensitive, quick, in situ Pathogen detection technology is researched and developed, is the problem of food security protection is in the urgent need to address.
The method of traditional detection pathogenic bacteria depends on specific microorganism and biochemical identification, because it is higher steady
It is qualitative to be widely used in hospital and biology laboratory.But traditional sensing techniques operating process is complicated, time-consuming it is necessary to have one
Determining the technical staff of professional knowledge could complete to operate, and can not meet portable, quick, in situ detection current demand.In recent years,
Due to the advantage such as wireless, passive, high sensitivity, simple to operate, in situ detection, magnetostrictive biosensor Pathogen detection technology
The extensive concern of numerous researchers is attracted.In the prior art, some sensors can only be fixed on somewhere in detection process, right
In fluid sample, germ could only be detected when germ passes through the position in liquid, so as to obtain containing for germ
Situation, it is exactly that contingency is larger the defects of this detection method maximum, especially for low concentration germ sample, even if sensor
Sensitivity is very high, if germ is not in contact with sensor, can not also play its function.In the prior art, the preparation energy also having
The sensor moved freely in prepare liquid, increase sensor and the contact probability of germ, increase Pathogen detection to a certain degree
Accuracy, but sensor moving process is slow, testing time length, is unfavorable for quick accurate detection.
The content of the invention
The present invention overcomes the shortcomings of the prior art, rapidly and accurately confirms water resource and other liquid by-products, such as
Whether lake, river, well water, running water, milk, fruit juice etc. have germ to pollute, and solve germ content in constant prepare liquid
The technical problem of quick detection, it is desirable to provide easily and fast, germ content device for fast detecting and detection side in accurate liquid
Method.
The present invention is achieved by following technical solution.
Germ content device for fast detecting in liquid, it includes detect tank, electrode, Helmholtz coil and washing pipe, institute
Stating electrode includes positive electrode and negative electrode, wherein：
Electrode is vertically arranged, and electrode upper end is conductor, and electrode bottom is insulator, and electrode upper surface is fixedly installed
Discharge opeing velar plate, electrode lower surface are provided with handle, and electrode upper end is fixedly installed some limited blocks along electrode circumferential direction,
The limited block includes upper limit position block and lower position block, and upper limit position block is be arranged in parallel with lower position block and vertical projection position weight
Close, be arranged with monitor station on the electrode between upper limit position block and lower position block, on monitor station with limited block position correspondence
Offer first through hole, monitor station upper surface is provided with sensor, and sensor is ribbon, and sensor surface is from inside to outside successively
Uniform sputter chromium layer and alloying element layer, alloying element layer are fixed with antibody layer outside, are also provided with sensor and limited block shape
The through hole of shape identical second, monitor station top pass through spring and connect through hole cover plate, and through hole cover plate diameter is more than the diameter of monitor station
And less than the diameter of discharge opeing velar plate；
Detection trench bottom is provided with two leakage fluid drams, and the diameter of leakage fluid dram is less than the diameter of discharge opeing velar plate, and discharge opeing
The diameter of mouth is more than the diameter of through hole cover plate, and sealing ring is fixedly installed at leakage fluid dram, and sealing ring was with monitor station contact position
Be full of cooperation, positive electrode is disposed therein at a leakage fluid dram, and negative electrode is arranged at another leakage fluid dram, positive electrode conductor portion with
Positive source is connected, and negative electrode conductor portion is connected with power cathode；
Washing pipe is provided with below detect tank, washing pipe both ends port is respectively set to water inlet and delivery port, washing pipe
Surface has been vertically arranged guide thimble with being provided with third through-hole at monitor station position correspondence, with washing tube axis direction, leads
Manage and extend in detect tank through washing to sleeve pipe, guide thimble side wall limits with being provided with groove at limited block position correspondence
Position block band moving electrode moves up and down in groove；Be provided with monitor station base in washing pipe, Helmholtz coil include inner ring with
Outer ring, monitor station base are arranged in Helmholtz coil, and the inner ring is connected with dc source, the outer ring and AC power
Connection.
Further, the sensor upper surface and monitor station lower surface are additionally provided with spacing with limited block position contact position
Pit.
Further, the sensor material is magnetostriction materials, and its magnetostriction coefficient is more than 20ppm (10-6)。
Further, it is close to the sensor surface and is also coated with material as copper or the protective layer of aluminium.
Further, the chromium layer and alloying element layer coating thickness are 60 ~ 150nm.
Further, a variety of different types of antibody are carried on the antibody layer.
Further, the detection groove sidewall is provided with scale.
The detection method of germ content device for fast detecting, is carried out successively as follows in liquid：
A, positive electrode and negative electrode are rotated, limited block is arranged in groove, then passes through positive electrode respectively with negative electrode
Wear guide thimble and extend in detect tank；
B, electrode assembles：First, sensor is arranged above monitor station, and covers disposed on sensor is put first through hole,
Overlapped simultaneously with limited block position on the second through hole set on monitor station, sensor is sheathed on electrode with monitor station；Then,
Synchronous rotary sensor and monitor station so that first through hole, the second through hole are misaligned with limited block position, and upper limit position block is with
Sensor is fixed on monitor station by limited block；Finally, through hole cover plate is connected by spring in monitor station upper surface, in electrode tip
Discharge opeing velar plate is fixedly mounted in portion；
C, fixed volume prepare liquid is added into detect tank；
D, electrode is powered：Startup power supply, power supply turn on, positively charged aggregation at positive electrode, negatron aggregation at negative electrode,
Electronegative germ is carried in prepare liquid at positive electrode to assemble, and electropositive germ is carried in prepare liquid at negative electrode
The antibody layer of aggregation, germ and sensor surface is specifically bound, and germ is attached in antibody layer surface, sensor mass
Increase, sensor intrinsic frequency change；
E, electrode pull-out detect tank is passed through by following steps by handle successively：Electrode pulls out, and electrode passes through discharge opeing cover plate
Monitor station is driven to pull out detect tank, discharge opeing velar plate closes leakage fluid dram；Electrode persistently pulls out, and electrode drive limited block drives inspection
Scaffold tower is moved in washing pipe, and through hole cover plate closes third through-hole；Electrode persistently pulls out, and electrode drive limited block drives detection
Platform is moved in monitor station base；
F, washing pipe（12）Inside it is passed through low pressure clear water, cleaning sensor（8）Specificity knot does not occur with antibody layer for surface
The germ of conjunction；
G, the inner ring connection dc source of Helmholtz coil, there is provided horizontal direction is not more than 200 Oersted magnetic field intensity
Steady magnetic field；The outer ring connection AC power of Helmholtz coil, there is provided horizontal direction is no more than 5 Oersted magnetic field intensity
Alternating magnetic field；DC current size and AC current frequency are adjusted, as Helmholtz coil amplitude and frequency change, detection passes
Sensor intrinsic frequency changes, and quantitatively obtains the content of germ in testing liquid；
H, after detection terminates, high pressure clear water, cleaning monitor station and monitor station base is passed through into washing pipe, finally, is passed through
Handle drives discharge opeing velar plate discharges detect tank away from leakage fluid dram.
The present invention has the advantages that compared with prior art.
Germ content device for fast detecting and its detection method in liquid provided by the invention, by adjusting adjustable perseverance（Hand over）
Stream source, there is provided alternating current and direct current biasing, by adjusting constant current size and a-c cycle, adjust magnetostrictor frequency and
Amplitude, the characteristics of magnetostriction materials intrinsic frequency changes in Helmholtz coil is taken full advantage of, passes through the thing of material in itself
The combination of characteristic and Biological Knowledge is managed, quickly realizes the quantitative detection of germ in testing liquid.
Brief description of the drawings
Fig. 1 is overall structure half-section diagram of the present invention.
In figure, 1 is detect tank, and 2 be electrode, and 3 be Helmholtz coil, and 4 be discharge opeing velar plate, and 5 be handle, and 6 be spacing
Block, 7 be monitor station, and 8 be sensor, and 9 be spring, and 10 be through hole cover plate, and 11 be sealing ring, and 12 be washing pipe, and 13 be guide sleeve
Pipe, 14 be monitor station base.
Embodiment
As shown in figure 1, germ content device for fast detecting in liquid, it includes detect tank 1, electrode 2, Helmholtz coil
3 include positive electrode and negative electrode with washing pipe 12, the electrode 2, wherein：
Electrode 2 is vertically arranged, and the upper end of electrode 2 is conductor, and the bottom of electrode 2 is insulator, and the upper surface of electrode 2 is fixed and set
Discharge opeing velar plate 4 is equipped with, the lower surface of electrode 2 is provided with handle 5, if the upper end of electrode 2 is fixedly installed along electrode circumferential direction
Dry limited block 6, the limited block 6 include upper limit position block and lower position block, and upper limit position block be arranged in parallel and erected with lower position block
Straight projected position overlaps, and is arranged with monitor station 7 on the electrode 2 between upper limit position block and lower position block, on monitor station 7 with limit
First through hole is offered at the position correspondence of block 6 of position, the upper surface of monitor station 7 is provided with sensor 8, and sensor 8 is ribbon, sensing
Uniform sputter chromium layer and alloying element layer, alloying element layer are fixed with antibody layer, sensor 8 to the surface of device 8 outside successively from inside to outside
On be also provided with and pass through spring 9 with the spacing block-shaped through hole of identical second, the top of monitor station 7 and connect through hole cover plate 10, through hole
The diameter of cover plate 10 is more than the diameter of monitor station 7 and less than the diameter of discharge opeing velar plate 4；
The bottom of detect tank 1 is provided with two leakage fluid drams, and the diameter of leakage fluid dram is less than the diameter of discharge opeing velar plate 4, and arranges
The diameter of liquid mouth is more than the diameter of through hole cover plate 10, is fixedly installed sealing ring 11 at leakage fluid dram, sealing ring 11 connects with monitor station 7
Synapsis is interference fit, and positive electrode is disposed therein at a leakage fluid dram, and negative electrode is arranged at another leakage fluid dram, positive electrode
Conductor portion is connected with positive source, and negative electrode conductor portion is connected with power cathode；
The lower section of detect tank 1 is provided with washing pipe 12, and washing pipe 12 both ends port is respectively set to water inlet and delivery port, washed
The surface of pipe 12 is washed with being provided with third through-hole at the position correspondence of monitor station 7, has been vertically arranged and has led with the washing axis direction of pipe 12
To sleeve pipe 13, guide thimble 13 is through washing pipe 12 and extends in detect tank 1, the side wall of guide thimble 13 and the position of limited block 6
Corresponding position is provided with groove, and limited block 6 moves up and down with moving electrode 2 in groove；Monitor station base is provided with washing pipe 12
14, Helmholtz coil 3 includes inner ring and outer ring, and monitor station base 14 is arranged in Helmholtz coil 3, the inner ring with it is straight
Power supply connection is flowed, the outer ring is connected with AC power.
Further, the upper surface of sensor 8 and the lower surface of monitor station 7 are also set up limited with limited block position contact position
Position pit.
Further, the material of sensor 8 is magnetostriction materials, and its magnetostriction coefficient is more than 20ppm (10-6)。
Further, the surface of sensor 8 of being close to is also coated with material as copper or the protective layer of aluminium.
Further, the chromium layer and alloying element layer coating thickness are 60 ~ 150nm.
Further, a variety of different types of antibody are carried on the antibody layer.
Further, the side wall of detect tank 1 is provided with scale.
The detection method of germ content device for fast detecting, is carried out successively as follows in liquid：
A, positive electrode and negative electrode are rotated, limited block 6 is arranged in groove, then passes through positive electrode respectively with negative electrode
Wear guide thimble 13 and extend in detect tank 1；
B, electrode assembles：First, sensor 8 is arranged at the top of monitor station 7, and set on sensor 8 first is led to
Overlapped simultaneously with the position of limited block 6 on the second through hole set on hole, monitor station 7, sensor 8 is sheathed on electrode 2 with monitor station 7
On；Then, synchronous rotary sensor 8 and monitor station 7 so that first through hole, the second through hole are misaligned with the position of limited block 6,
Sensor 8 is fixed on monitor station 7 by upper limit position block with lower position block；Finally, connected in the upper surface of monitor station 7 by spring 9
Through hole cover plate 10, discharge opeing velar plate 4 is fixedly mounted in the end of electrode 2；
C, fixed volume prepare liquid is added into detect tank 1；
D, electrode is powered：Startup power supply, power supply turn on, positively charged aggregation at positive electrode, negatron aggregation at negative electrode,
Electronegative germ is carried in prepare liquid at positive electrode to assemble, and electropositive germ is carried in prepare liquid at negative electrode
Aggregation, germ and the antibody layer on the surface of sensor 8 are specifically bound, and germ is attached in antibody layer surface, the matter of sensor 8
Amount increase, the intrinsic frequency of sensor 8 change；
E, electrode 2 is pulled out by detect tank 1 by handle 5 and passes through following steps successively：Electrode 2 pulls out, and electrode 2 passes through discharge opeing
Cover plate 4 drives monitor station 7 to pull out detect tank 1, and discharge opeing velar plate 4 closes leakage fluid dram；Electrode 2 persistently pulls out, the driving limit of electrode 2
Position block 6 drives monitor station 7 to move in washing pipe 12, and through hole cover plate 10 closes third through-hole；Electrode 2 persistently pulls out, electrode 2
Driving limited block 6 drives monitor station 7 to move in monitor station base 14；
F, washing pipe（12）Inside it is passed through low pressure clear water, cleaning sensor（8）Specificity knot does not occur with antibody layer for surface
The germ of conjunction；
G, the inner ring connection dc source of Helmholtz coil 3, there is provided horizontal direction is not more than 200 Oersted magnetic field intensity
Steady magnetic field；The outer ring connection AC power of Helmholtz coil 3, there is provided horizontal direction is not more than 5 Oersted magnetic field intensity
Alternating magnetic field；DC current size and AC current frequency are adjusted, as the amplitude of Helmholtz coil 3 and frequency change, inspection
Survey the intrinsic frequency of sensor 8 to change, quantitatively obtain the content of germ in testing liquid；
H, after detection terminates, high pressure clear water, cleaning monitor station 7 and monitor station base 14 are passed through into washing pipe 12, finally,
Discharge opeing velar plate 4 is driven to discharge detect tank away from leakage fluid dram by handle 5.
The present invention can be embodied without departing from the spiritual or substantive of invention in a variety of forms, it should therefore be appreciated that on
State embodiment and be not limited to foregoing details, and should widely be explained in claim limited range, therefore fall into right
It is required that or the change in its equivalent scope and remodeling all should be claim and covered.
Claims (8)
1. germ content device for fast detecting in liquid, it includes detect tank（1）, electrode（2）, Helmholtz coil（3）With wash
Wash pipe（12）, the electrode（2）Including positive electrode and negative electrode, it is characterised in that：
Electrode（2）It is vertically arranged, electrode（2）Upper end is conductor, electrode（2）Bottom is insulator, electrode（2）Upper surface is consolidated
Surely it is provided with discharge opeing velar plate（4）, electrode（2）Lower surface is provided with handle（5）, electrode（2）Upper end is along electrode circumferential direction
It is fixedly installed some limited blocks（6）, the limited block（6）Including upper limit position block and lower position block, upper limit position block and lower limit
Block be arranged in parallel and vertical projection position overlaps, the electrode between upper limit position block and lower position block（2）On be arranged with inspection
Scaffold tower（7）, monitor station（7）Upper and limited block（6）First through hole, monitor station are offered at position correspondence（7）Upper surface is provided with
Sensor（8）, sensor（8）For ribbon, sensor（8）Surface uniform sputter chromium layer and gold element successively from inside to outside
Layer, alloying element layer are fixed with antibody layer, sensor outside（8）On be also provided with and the spacing block-shaped through hole of identical second, detection
Platform（7）Top passes through spring（9）Connect through hole cover plate（10）, through hole cover plate（10）Diameter is more than monitor station（7）Diameter and
Less than discharge opeing velar plate（4）Diameter；
Detect tank（1）Bottom is provided with two leakage fluid drams, and the diameter of leakage fluid dram is less than discharge opeing velar plate（4）Diameter, and arrange
The diameter of liquid mouth is more than through hole cover plate（10）Diameter, be fixedly installed sealing ring at leakage fluid dram（11）, sealing ring（11）With inspection
Scaffold tower（7）Contact position is interference fit, and positive electrode is disposed therein at a leakage fluid dram, and negative electrode is arranged at another leakage fluid dram
Place, positive electrode conductor portion are connected with positive source, and negative electrode conductor portion is connected with power cathode；
Detect tank（1）Lower section is provided with washing pipe（12）, washing pipe（12）Both ends port is respectively set to water inlet and delivery port,
Washing pipe（12）Surface and monitor station（7）Third through-hole is provided with position correspondence, is managed with washing（12）Axis direction is vertically
It is provided with guide thimble（13）, guide thimble（13）Managed through washing（12）And extend to detect tank（1）It is interior, guide thimble（13）
Side wall and limited block（6）Groove, limited block are provided with position correspondence（6）Band moving electrode（2）Moved up and down in groove；Washing
Pipe（12）Inside it is provided with monitor station base（14）, Helmholtz coil（3）Including inner ring and outer ring, monitor station base（14）Set
In Helmholtz coil（3）Interior, the inner ring is connected with dc source, and the outer ring is connected with AC power.
2. germ content device for fast detecting in liquid according to claim 1, it is characterised in that：The sensor（8）
Upper surface and monitor station（7）Lower surface is additionally provided with spacing pit with limited block position contact position.
3. germ content device for fast detecting in liquid according to claim 1, it is characterised in that：The sensor（8）
Material is magnetostriction materials, and its magnetostriction coefficient is more than 20ppm (10-6)。
4. germ content device for fast detecting in liquid according to claim 3, it is characterised in that：It is close to the sensor
（8）Surface is also coated with material as copper or the protective layer of aluminium.
5. germ content device for fast detecting in liquid according to claim 3, it is characterised in that：The chromium layer and
Alloying element layer coating thickness is 60 ~ 150nm.
6. germ content device for fast detecting in liquid according to claim 3, it is characterised in that：Taken on the antibody layer
With a variety of different types of antibody.
7. germ content device for fast detecting in liquid according to claim 1, it is characterised in that：The detect tank（1）
Side wall is provided with scale.
8. the detection method of germ content device for fast detecting in liquid as claimed in claim 1, it is characterised in that by as follows
Step is carried out successively：
A, positive electrode and negative electrode are rotated, makes limited block（6）It is arranged in groove, then extends through positive electrode and negative electrode
Guide thimble（13）And extend to detect tank（1）It is interior；
B, electrode assembles：First, by sensor（8）It is arranged at monitor station（7）Top, and by sensor（8）The first of upper setting
Through hole, monitor station（7）On second through hole of upper setting simultaneously and limited block（6）Position overlaps, sensor（8）With monitor station（7）
It is sheathed on electrode（2）On；Then, synchronous rotary sensor（8）With monitor station（7）So that first through hole, the second through hole with limit
Position block（6）Position is misaligned, and upper limit position block and lower position block are by sensor（8）It is fixed on monitor station（7）On；Finally, detecting
Platform（7）Upper surface passes through spring（9）Connect through hole cover plate（10）, in electrode（2）Discharge opeing velar plate is fixedly mounted in end（4）；
C, to detect tank（1）Middle addition fixed volume prepare liquid；
D, electrode is powered：Startup power supply, power supply turn on, positively charged aggregation at positive electrode, negatron aggregation at negative electrode, to be measured
Electronegative germ is carried in liquid at positive electrode to assemble, and electropositive germ is carried in prepare liquid to gathering at negative electrode
Collection, germ and sensor（8）The antibody layer on surface is specifically bound, and germ is attached in antibody layer surface, sensor（8）
Quality increase, sensor（8）Intrinsic frequency changes；
E, handle is passed through（5）By electrode（2）Pull out detect tank（1）Pass through following steps successively：Electrode（2）Pull out, electrode（2）It is logical
Cross discharge opeing cover plate（4）Drive monitor station（7）Pull out detect tank（1）, discharge opeing velar plate（4）Leakage fluid dram is closed；Electrode（2）Continue
Pull out, electrode（2）Drive limited block（6）Drive monitor station（7）Move to washing pipe（12）It is interior, through hole cover plate（10）By threeway
Hole closes；Electrode（2）It is lasting to pull out, electrode（2）Drive limited block（6）Drive monitor station（7）Move to monitor station base（14）
In；
F, washing pipe（12）Inside it is passed through low pressure clear water, cleaning sensor（8）Surface is not specifically bound with antibody layer
Germ；
G, Helmholtz coil（3）Inner ring connection dc source, there is provided horizontal direction is no more than 200 Oersted magnetic field intensity
Steady magnetic field；Helmholtz coil（3）Outer ring connection AC power, there is provided horizontal direction is not more than 5 Oersted magnetic field intensity
Alternating magnetic field；DC current size and AC current frequency are adjusted, with Helmholtz coil（3）Amplitude and frequency change,
Detection sensor（8）Intrinsic frequency changes, and quantitatively obtains the content of germ in testing liquid；
H, after detection terminates, managed to washing（12）High pressure clear water is inside passed through, cleans monitor station（7）With monitor station base（14）, most
Afterwards, handle is passed through（5）Drive discharge opeing velar plate（4）Away from leakage fluid dram, detect tank is discharged.
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| CN108802086A (en) * | 2018-04-17 | 2018-11-13 | 太原理工大学 | Magnetic shakes effect trace sensor |
| CN108932623B (en) * | 2018-09-04 | 2020-09-22 | 深圳砺剑脑科学科技有限公司 | Intelligent anti-counterfeiting system |
| CN109406617B (en) * | 2018-10-23 | 2022-06-24 | 太原科技大学 | Electromagnetic effect-based germ detection device and detection method thereof |
| CN109884298B (en) * | 2019-03-15 | 2021-10-01 | 太原科技大学 | Drum-type detection device and detection method for content of germs in liquid |
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