CN107047285B - Breeding method of ornamental colored rice variety - Google Patents
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- A—HUMAN NECESSITIES
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Abstract
The invention discloses a breeding method of ornamental colored rice varieties, and belongs to the technical field of ornamental rice breeding. The invention selects disease-resistant dwarf green leaf rice as recurrent parent R, which is respectively hybridized with purple leaf rice, yellow leaf rice, stripe leaf rice and yellow green leaf rice, and the progeny is backcrossed with recurrent parent for multiple generations; and breeding near isogenic lines with agronomic characters similar to recurrent parent and target leaf color characters, and planting according to a certain pattern to obtain the rice field picture. The rice plants with different colors, the shapes, the agronomic characters and the growth periods of the ornamental colored rice varieties bred by the method disclosed by the invention are uniform and consistent in expression after being planted, and the rice field patterns at each stage of the growth and development of the rice are clear in color gradation and stable in pattern effect.
Description
Technical Field
The invention particularly relates to a breeding method of ornamental colored rice varieties, and belongs to the technical field of ornamental rice breeding.
Background
The rice field painting is originally an artistic form of Japan, and farmers can plant various rice varieties to draw in the rice field. The method mainly comprises the steps of planting green and purple leaf rice in a paddy field, fixing coordinates according to a pattern by using a traditional scriber in the paddy field during planting, drawing the line to draw the outline of the pattern or a character, and finally planting purple seedlings, wherein the purple seedlings can present pre-planned patterns or characters along with the growth of the rice. Every year, the Japan Qingson county house village holds a 'rice field art festival' to show rice field paintings made by farmers so as to attract tourists. The current rice field pictures appear all over the world. In recent years, creative paddy field painting planting performed by farmers is reported in various parts of China. People can draw works such as butterflies, portraits, rainbow and the like through planting the colorful rice when the rice is used as a pen and the paddy field is used as paper, thereby adding unique ecological flavor to the leisure sightseeing agriculture. As the rice grows, the rice field patterns become clearer, and when the rice enters the period from stem jointing to ear sprouting, the rice is also the best ornamental period of the artistic colored field. However, the rice varieties used for rice field painting planting are all existing varieties with leaves of different colors, the growth periods, the plant heights and the plant types of the varieties are different, so that the rice field painting has large expressive force difference in each growth stage of the rice, and the optimal and stable expressive effect cannot be achieved. The invention selects the green leaf rice variety with disease resistance, short stalk and medium growth period as the recurrent parent, and is hybridized and backcrossed with the purple leaf rice variety, yellow leaf rice variety, stripe leaf rice variety and yellow green leaf rice variety, and the near isogenic line with growth period, plant height and other agronomic characters similar to those of the recurrent parent is bred, and has target leaf color character, the near isogenic line is characterized in that the rice field painting rice plant shape, the agronomic characters and the growth period are orderly and consistent, and the rice field painting pattern shows the advantages of clear color gradation and stable pattern effect at each stage of rice growth and development.
Disclosure of Invention
The invention aims to provide a breeding method of ornamental colored rice varieties.
The invention selects disease-resistant dwarf green leaf rice as recurrent parent R, which is hybridized with purple leaf rice, yellow leaf rice, stripe leaf rice and yellow green leaf rice, the offspring is backcrossed with recurrent parent for multiple generations, near isogenic line strains with agronomic character similar to that of recurrent parent and target leaf color character are bred, and the rice field picture can be planted according to certain pattern.
The technical scheme of the invention is as follows:
1) selecting disease-resistant semi-short stalk non-photosensitive medium growth period green leaf rice variety as recurrent parent R, selecting purple leaf rice variety IR1552(A), yellow leaf rice variety topaz B (B), rice streak mosaic mutant st (t) (C) and yellow green leaf rice variety ygl10(D) as leaf color character leading-in parent, and making them into hybrid R/A, R/B, R/C, R/D to obtain hybrid F1Respectively take F as substitute1-A、F1-B、F1-C、F1-DRepresents;
2) planting F1-A、F1-B、F1-C、F1-DBackcrossing the recurrent parent R at the flowering period to obtain backcross F1 generations respectively by BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DRepresents;
3) planting BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DSeparately harvest to obtain BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-D；
4) Planting BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-DScreening target characters in seedling stage, wherein BC1F2-AScreening for the plants with purple leaf character and BC1F2-BScreening for BC with yellow leaf character1F2-CScreening for BC with leaf streak character1F2-DScreening 200 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 10 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-D；
5) Plant planting BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-DScreening 5 plants in a cell with prominent target character expression in the flowering period, and backcrossing the 5 plants with the recurrent parent R to obtain backcross 2 generation BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-D；
6) Planting BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-DScreening target characters in seedling stage, wherein BC2F1-AScreening for the plants with purple leaf character and BC2F1-BScreening for BC with yellow leaf character2F1-CScreening for BC with leaf streak character2F1-DScreening 50 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 10 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-D；
7) Planting BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-DScreening target characters in seedling stage, wherein BC2F2-AScreening for the plants with purple leaf character and BC2F2-BScreening for BC with yellow leaf character2F2-CScreening for BC with leaf streak character2F2-DScreening 20 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 8 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC2F3-A、BC2F3-B、BC2F3-C、BC2F3-D；
8) Continuing the steps 5), 6) and 7) until BC is obtained4F3-A、BC4F3-B、BC4F3-C、BC4F3-DPlanting BC4F3-A、BC4F3-B、BC4F3-C、BC4F3-DScreening 1 brand marks of each plant with similar agronomic characters and growth periods as recurrent parent R in each region in the flowering period, and having corresponding target characters, and harvesting seeds in the mature period to breed 4 near-isogenic lines with leaf colors: purple leaf R-A, yellow leaf R-B, leaf of striped speckles R-C, and yellow green leaf R-D;
9) purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D respectively propagate seeds, R-A, R-B, R-C, R-D and recurrent parent R have 5 different leaf colors, and the rice field painting can be planted by utilizing the purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D and utilizing the purple leaf R-8932-B, R-C, R-D and recurrent parent R to plant in A certain pattern jigsaw.
The recurrent parent R is: phoenix No. 1, rice No. 10 or IRl 3635-38.
Advantageous effects
The 4 ornamental colored rice varieties bred by the method and the recurrent parent are near isogenic lines, the agronomic characters such as the growth period and the plant height are very similar, the near isogenic lines respectively have target leaf color characters, the rice field planted by the near isogenic lines according to a certain pattern shows regular and consistent rice plant shape, agronomic character and growth period, the near isogenic lines resist diseases, and the rice field patterns at all stages of the growth and development of rice show the advantages of clear color gradation and stable pattern effect.
The specific implementation mode is as follows:
example 1:
1) selecting disease-resistant semi-short-stalk non-photosensitive medium growth period green leaf rice variety Fioneng No. 1 as recurrent parent R, selecting purple leaf rice variety IR1552(A), yellow leaf rice variety topaz B (B), rice stripe mosaic mutant st (t) (C), yellow green leaf rice variety ygl10(D) as leaf color character leading-in parent, and making into hybrid R/A, R/B, R/C, R/D to obtain hybrid F1Respectively take F as substitute1-A、F1-B、F1-C、F1-DRepresents;
2) planting F1-A、F1-B、F1-C、F1-DBackcrossing the recurrent parent R at the flowering period to obtain backcross F1 generations respectively by BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DRepresents;
3) planting BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DSeparately harvest to obtain BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-D；
4) Planting BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-DScreening target characters in seedling stage, wherein BC1F2-AScreening for the plants with purple leaf character and BC1F2-BScreening for BC with yellow leaf character1F2-CScreening for BC with leaf streak character1F2-DScreening 200 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 10 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-D；
5) Plant planting BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-DScreening 5 plants in a cell with prominent target character expression in the flowering period, and backcrossing the 5 plants with the recurrent parent R to obtain backcross 2 generation BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-D；
6) Planting BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-DScreening target characters in seedling stage, wherein BC2F1-AScreening for the plants with purple leaf character and BC2F1-BScreening for BC with yellow leaf character2F1-CScreening for BC with leaf streak character2F1-DScreening 50 rice seedlings with yellow-green leaf character, planting in different regions, screening agricultural character and growth in different regions in mature periodHarvesting 10 plants of each individual plant with the breeding period similar to the recurrent parent R to obtain BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-D；
7) Planting BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-DScreening target characters in seedling stage, wherein BC2F2-AScreening for the plants with purple leaf character and BC2F2-BScreening for BC with yellow leaf character2F2-CScreening for BC with leaf streak character2F2-DScreening 20 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 8 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC2F3-A、BC2F3-B、BC2F3-C、BC2F3-D；
8) Continue with steps 5), 6), 7) to BC4F3-A、BC4F3-B、BC4F3-C、BC4F3-DPlanting BC4F3-A、BC4F3-B、BC4F3-C、BC4F3-DScreening 1 brand marks of each plant with similar agronomic characters and growth periods as recurrent parent R in each region in the flowering period, and having corresponding target characters, and harvesting seeds in the mature period to breed 4 near-isogenic lines with leaf colors: purple leaf R-A, yellow leaf R-B, leaf of striped speckles R-C, and yellow green leaf R-D;
9) purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D respectively propagate seeds, R-A, R-B, R-C, R-D and recurrent parent R have 5 different leaf colors, and the rice field painting can be planted by utilizing the purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D and utilizing the purple leaf R-8932-B, R-C, R-D and recurrent parent R to plant in A certain pattern jigsaw.
The 4 kinds of ornamental colored rice bred and the recurrent parent are near isogenic lines, the agronomic characters such as the growth period and the plant height are very similar, the near isogenic lines respectively have target leaf color characters, the rice field planted by the near isogenic lines according to a certain pattern shows regular and consistent rice plant drawing shape, agronomic characters and growth period, the near isogenic lines resist diseases, and the rice field drawing patterns at each stage of the growth and development of the rice have the advantages of clear color level and stable pattern effect.
Example 2:
1) selecting disease-resistant semi-short stalk non-sensitive medium growth period green leaf rice variety Lindao No. 10 as recurrent parent R, selecting purple leaf rice variety IR1552(A), yellow leaf rice variety topaz B (B), rice streak leaf mutant st (t) (C), yellow green leaf rice variety ygl10(D) as leaf color character leading-in parent, and making into hybrid R/A, R/B, R/C, R/D to obtain hybrid F1Respectively take F as substitute1-A、F1-B、F1-C、F1-DRepresents;
2) planting F1-A、F1-B、F1-C、F1-DBackcrossing the recurrent parent R at the flowering period to obtain backcross F1 generations respectively by BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DRepresents;
3) planting BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DSeparately harvest to obtain BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-D；
4) Planting BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-DScreening target characters in seedling stage, wherein BC1F2-AScreening for the plants with purple leaf character and BC1F2-BScreening for yellow leaf characterForm, BC1F2-CScreening for BC with leaf streak character1F2-DScreening 200 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 10 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-D；
5) Plant planting BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-DScreening 5 plants in a cell with prominent target character expression in the flowering period, and backcrossing the 5 plants with the recurrent parent R to obtain backcross 2 generation BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-D；
6) Planting BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-DScreening target characters in seedling stage, wherein BC2F1-AScreening for the plants with purple leaf character and BC2F1-BScreening for BC with yellow leaf character2F1-CScreening for BC with leaf streak character2F1-DScreening 50 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 10 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-D；
7) Planting BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-DScreening target characters in seedling stage, wherein BC2F2-AScreening for the plants with purple leaf character and BC2F2-BScreening for BC with yellow leaf character2F2-CScreening for BC with leaf streak character2F2-DScreening 20 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 8 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC2F3-A、BC2F3-B、BC2F3-C、BC2F3-D；
8) Continue with steps 5), 6), 7) to BC4F3-A、BC4F3-B、BC4F3-C、BC4F3-DPlanting BC4F3-A、BC4F3-B、BC4F3-C、BC4F3-DScreening 1 brand marks of each plant with similar agronomic characters and growth periods as recurrent parent R in each region in the flowering period, and having corresponding target characters, and harvesting seeds in the mature period to breed 4 near-isogenic lines with leaf colors: purple leaf R-A, yellow leaf R-B, leaf of striped speckles R-C, and yellow green leaf R-D;
9) purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D respectively propagate seeds, R-A, R-B, R-C, R-D and recurrent parent R have 5 different leaf colors, and the rice field painting can be planted by utilizing the purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D and utilizing the purple leaf R-8932-B, R-C, R-D and recurrent parent R to plant in A certain pattern jigsaw.
The 4 kinds of ornamental colored rice bred are very similar to recurrent parents in agronomic characters such as growth period, plant height and the like, and respectively have target leaf color characters, the rice field planted by the near isogenic system according to a certain pattern shows regular and consistent rice plant drawing shape, agronomic characters and growth period, is disease-resistant, and the rice field drawing pattern shows the advantages of clear color gradation and stable pattern effect at each stage of rice growth and development.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (2)
1. A method for breeding ornamental colored rice varieties is characterized in that: the breeding method comprises the following specific steps:
1) selecting disease-resistant semi-dwarf non-sensitive medium growth period green leaf rice variety as recurrent parent R, selecting purple leaf rice variety IR1552 as parent A, yellow leaf rice variety topaz B as parent B, rice streak leaf mutant st (t) as parent C, yellow green leaf rice variety ygl10 as parent D, using parent A, B, C, D as leaf color character leading-in parent, and making hybridization R/A, R/B, R/C, R/D to obtain hybridization F1Respectively take F as substitute1-A、F1-B、F1-C、F1-DRepresents;
2) planting F1-A、F1-B、F1-C、F1-DBackcrossing the recurrent parent R at the flowering period to obtain backcross F1 generations respectively by BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DRepresents;
3) planting BC1F1-A、BC1F1-B、BC1F1-C、BC1F1-DSeparately harvest to obtain BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-D；
4) Planting BC1F2-A、BC1F2-B、BC1F2-C、BC1F2-DScreening target characters in seedling stage, wherein BC1F2-AScreening for the plants with purple leaf character and BC1F2-BScreening for BC with yellow leaf character1F2-CScreening for BC with leaf streak character1F2-DScreening for yellowPlanting 200 rice seedlings with green leaf character in different regions, screening 10 rice seedlings with agronomic character and growth period similar to that of recurrent parent R in each region in mature period, and harvesting to obtain BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-D；
5) Plant planting BC1F3-A、BC1F3-B、BC1F3-C、BC1F3-DScreening 5 plants in a cell with prominent target character expression in the flowering period, and backcrossing the 5 plants with the recurrent parent R to obtain backcross 2 generation BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-D；
6) Planting BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-DScreening target characters in seedling stage, wherein BC2F1-AScreening for the plants with purple leaf character and BC2F1-BScreening for BC with yellow leaf character2F1-CScreening for BC with leaf streak character2F1-DScreening 50 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 10 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-D；
7) Planting BC2F2-A、BC2F2-B、BC2F2-C、BC2F2-DScreening target characters in seedling stage, wherein BC2F2-AScreening for the plants with purple leaf character and BC2F2-BScreening for BC with yellow leaf character2F2-CScreening for BC with leaf streak character2F2-DScreening 20 rice seedlings with yellow-green leaf characteristics, planting in different regions, screening 8 rice seedlings with agronomic characteristics and growth period similar to those of recurrent parent R in each region in mature period, and harvesting to obtain BC2F3-A、BC2F3-B、BC2F3-C、BC2F3-D；
8) Continuing the steps 5), 6) and 7) until BC is obtained4F3-A、BC4F3-B、BC4F3-C、BC4F3-DPlanting BC4F3-A、BC4F3-B、BC4F3-C、BC4F3-DScreening 1 brand marks of each plant with similar agronomic characters and growth periods as recurrent parent R in each region in the flowering period, and having corresponding target characters, and harvesting seeds in the mature period to breed 4 near-isogenic lines with leaf colors: purple leaf R-A, yellow leaf R-B, leaf of striped speckles R-C, and yellow green leaf R-D;
9) purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D respectively propagate seeds, R-A, R-B, R-C, R-D and recurrent parent R have 5 different leaf colors, and the rice field painting can be planted by utilizing the purple leaf R-A, yellow leaf R-B, striped leaf R-C and yellow green leaf R-D and utilizing the purple leaf R-8932-B, R-C, R-D and recurrent parent R to plant in A certain pattern jigsaw.
2. The method for breeding ornamental colored rice variety according to claim 1, wherein the method comprises the following steps: the recurrent parent R is: phoenix No. 1 or rice No. 10.
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| CN108703067B (en) * | 2018-04-28 | 2020-07-28 | 江西省农业科学院水稻研究所 | Rapid breeding method of ornamental rice variety in growth period |
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