CN107043696A - Automatically continuously culture detects screening plant to microorganism - Google Patents

Automatically continuously culture detects screening plant to microorganism Download PDF

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Publication number
CN107043696A
CN107043696A CN201710310028.9A CN201710310028A CN107043696A CN 107043696 A CN107043696 A CN 107043696A CN 201710310028 A CN201710310028 A CN 201710310028A CN 107043696 A CN107043696 A CN 107043696A
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moving plate
rotating disk
level
pull
price fixing
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CN201710310028.9A
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张强
张利娟
周芳
吴建丽
李慧川
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Huanghe Science and Technology College
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Huanghe Science and Technology College
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Priority to CN201710310028.9A priority Critical patent/CN107043696A/en
Publication of CN107043696A publication Critical patent/CN107043696A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Automatic splinter screening device for screening is detected the invention discloses a kind of microorganism continuous culture, including rotating mechanism, drive mechanism and testing agency, and charging aperture and discharging opening, the holster shell of base and closing is additionally provided with, temperature control system and air distribution system and humidity control system are provided with holster shell;Rotating mechanism includes one-level rotating disk mechanism, secondary turntable mechanism and three-level rotating disk mechanism from inside to outside, and every grade of rotating disk mechanism includes being evenly distributed with sample box locating slot on Moving plate and price fixing, Moving plate respectively, and gap is maintained between Moving plate and price fixing.The present invention is cultivated and is classified continuous detection to different microorganisms sample by the control to temperature, humidity and gas componant.A point mouth output is carried out automatically according to the detection characteristic such as microbial activity, and automatic record is carried out to each kit by way of barcode scanning achieving, to realize that microculture detection comprehensive automation provides technical foundation.

Description

Automatically continuously culture detects screening plant to microorganism
Technical field:
The invention belongs to microculture detection device technical field, and in particular to a kind of automatically continuously culture detection of microorganism Screening plant.
Background technology:
Microorganism include bacterium, virus, fungi and some small-sized protists, it individual it is small be difficult to detect by an unaided eye, With human relation closely, the numerous areas such as food, medicine, industrial or agricultural, environmental protection are related generally to.And it is raw to carry out culture to microorganism One kind in thing culture, human being's production, the microorganism of life are conducive to primarily to obtaining.Simultaneously as to microorganism It is veryer long in incubation, and easily influenceed by temperature, the concentration of nutrient solution, whole process can expend excessive people Power, material resources.For example, in the past as progress in Foods or drinkses whether the method for the judgement containing spoilage organisms, including make With the machines such as immune measurement method, ELISA method, gas-chromatography mass analysis, liquid chromatographic mass analytic approach survey method, but determine Pre-treatment is numerous and diverse, and the precision of judgement is insufficient.So as to generally take a long time to cultivate spoilage organisms.Bacterium, virus and Molecular biosciences detection field, the process of bacterial micro-organism culture is particularly significant, the result and accuracy of every detection for after There is strong influence, but be due to the environmental requirements such as temperature height, humidity difference required for various bacterial micro-organism cultures It is not quite similar, generally requires manually to found required environment when carrying out molecular biosciences and Bacteria Culture, once need the thin of culture Bacterium and microbe species are more, then are easy to cause medical worker's workload to increase and be likely to cause operational error, to medical matters Personnel bring very big inconvenience.
Existing microbial cultivation device is mainly cultivated microorganism by the way of temperature control, and whole process still can consume Take excessive human and material resources resource and automaticity is low, for those skilled in the art, need to be developed a kind of energy What is enough automated cultivates microorganism, and can save the microbial cultivation device of human and material resources resource to a greater extent again. The weak point of existing microculture detection device generally existing is the overlong time of culture, when how to shorten culture Between, improve efficiency turn into need break through bottleneck.The content of the invention:
There is structure type unification for current existing microculture or detection device and be unfavorable for automatically continuously in the present invention There is provided a kind of microorganism that operation is very easy automation culture detection device for the problem of culture and screening and deficiency.
Technical scheme:A kind of microorganism continuous culture detects automatic splinter screening device for screening, including rotating mechanism, drive mechanism and inspection Mechanism, and charging aperture and discharging opening are surveyed, the holster shell of base and closing is additionally provided with, is provided with the holster shell Temperature control system and air distribution system and humidity control system;Rotating mechanism include it is adjacent successively from inside to outside and be distributed with one heart one Level rotating disk mechanism, secondary turntable mechanism and three-level rotating disk mechanism, every grade of rotating disk mechanism include Moving plate and price fixing, the price fixing respectively It is fixed on base or support, sample box locating slot is evenly distributed with the Moving plate, gap is maintained between Moving plate and price fixing, Sample box locating slot is connected with gap;Moving plate and price fixing are installed by bearing or by together with track installation, meeting Moving plate It is able to can be rotated on price fixing;Each output end drive connection of each Moving plate respectively with drive mechanism is so as to keep Moving plates at different levels same Step or asynchronous rotation;It is multiple at least provided with a three class control mechanism between one-level rotating disk mechanism and secondary turntable mechanism It is uniformly distributed during three class control mechanism;At least provided with a Two-stage control machine between secondary turntable mechanism and three-level rotating disk mechanism Structure, is uniformly distributed during multiple Two-stage control mechanisms;The three class control mechanism and Two-stage control mechanism are all in Moving plate and price fixing Gap in Matching installation have baffle plate, be provided with downcast hole on the price fixing immediately below baffle plate;Pass through simultaneously on the inside of frame Fixed seat is provided with the outer layer push-pull mechanism of radial arrangement, the outer layer push-and-pull boom end of outer layer push-pull mechanism and the baffle plate end It is fixed together, internal layer push-pull mechanism, the internal layer of internal layer push-pull mechanism is installed in the inner side of the outer layer pull bar or outer layer Push-and-pull boom end, which is provided with, cuts somebody's hair, and cuts somebody's hair on the upside of Moving plate and positioned at the positive upside of slide plate;At least set on three-level rotating disk mechanism An one-level controlling organization is put, is uniformly distributed during multiple one-level controlling organizations;The one-level controlling organization is in three-level disk machine Radially mounted on the upside of the Moving plate of structure to have push-pull mechanism, the push-and-pull boom end of the push-pull mechanism, which is provided with, cuts somebody's hair, and is respectively pushed away described The side of head is provided with barcode scanning head, cuts somebody's hair on the upside of Moving plate;Or the one-level controlling organization is in three-level rotating disk mechanism Downcast hole is provided with price fixing, barcode scanning head is provided with above downcast hole side;Machine corresponding with each one-level controlling organization Rack enclosure is provided with discharging opening at position;The lower end of feeding-passage is located on the upside of the one-level rotating disk mechanism, feeding-passage The charging aperture at end is located at the upper end of holster shell;The rotation of the three class control mechanism, Two-stage control mechanism and one-level controlling organization The upper region for turning direction is provided with microorganism sample testing agency.
Discharging opening is provided with the lateral wall of holster shell and outer cover is set, the bottom of the outer cover is provided with support Plate, the both sides of outer cover are open, and its side is provided with pusher, and opposite side is provided with stacking mechanism.Stacking mechanism includes one The piling platform parallel with shield base supporting plate, piling platform bottom is provided with telescoping drive mechanism and guiding mechanism.
The present invention has the advantages that:The present invention devises a kind of other rotating disk mechanism of multi-layer, by temperature, Continuous detection is cultivated and is classified in the control of humidity and gas componant to different microorganisms sample.Positioned at every grade of rotating disk mechanism On sample it is different by culture degree, various sample is detected and divided by testing agency respectively by every grade of rotating disk mechanism , can not only be by the way that the microbial reproduction situation of each sample box of detection be corresponded to after barcode scanning again to output, and extension can be passed through The mode of circulating path directly controls respective samples to receive the time of culture by way of dropping channel is excluded, and detects Go out the necessary testing result such as the activity ratio that different incubation times are formed.So as to which the present invention can be by the side that automatically controls Formula, which is realized, to be controlled the condition of culture of temperature, humidity and gas componant, and to individual by way of changing culture sample path The incubation time control of case, the output of point mouth is carried out automatically according to the detection characteristic such as microbial activity, and by way of barcode scanning pair Each kit carries out automatic record and achieved, to realize that microculture detection comprehensive automation provides technical foundation.
Brief description of the drawings
Fig. 1 is the surface structure schematic diagram of the present invention;
Fig. 2 is Fig. 1 A-A cross-sectional views;
Fig. 3 is the structural representation of one-level rotating disk mechanism in Fig. 2;
Fig. 4 is the schematic diagram of Tu2Zhong three class controls mechanism;
Fig. 5 is the schematic diagram of Tu2Zhong Two-stage controls mechanism;
Fig. 6 is one of structural representation of one-level controlling organization in Fig. 2;
Fig. 7 is the two of the structural representation of one-level controlling organization in Fig. 2;
Fig. 8 is the mplifying structure schematic diagram of controlling organization in Fig. 4.
Label 1 is one-level rotating disk mechanism in way, and 2 be secondary turntable mechanism, and 3 be three-level rotating disk mechanism, and 4 be feeding-passage, 5 It is Two-stage control mechanism for three class control mechanism, 6,7 be one-level controlling organization, and 8 be testing agency, and 91 be baffle plate, and 92 be connection Seat, 93 be sleeve pipe, and 94 be fixed seat, and 95 be outer layer push-pull mechanism, and 96 be outer layer pull bar, and 97 be internal layer pull bar, and 98 be to push away Head, 99 be barcode scanning head, and 10 be gear platform, and 11 be Moving plate, and 12 be price fixing, and 13 be glade plane space, and 14 be locating slot, and 15 be downcast hole, 16 be base, and 17 be holster shell, and 18 be charging aperture, and 19 be discharging opening outer cover, and 20 be pusher, and 21 be piling platform, 22 It is guide rod for telescoping drive mechanism, 23,24 be rib, and 25 be side stand, and 26 be sample box.
Embodiment:
Embodiment:A kind of outward appearance of microorganism continuous culture detection automatic splinter screening device for screening is as shown in figure 1, contain base and closing The parts such as holster shell.Temperature control system and air distribution system and humidity control system are provided with holster shell.On temperature control System, air distribution system, humidity control system, the testing agency 8 that microorganism is checked or analyzed, and micro-control unit, It can be replaced using existing controlling organization and testing agency 8, or freely arrange in pairs or groups and suitably repair in prior art basis Change relevant parameter to realize.
Discharging opening is provided with the lateral wall of holster shell and outer cover is set, the bottom of the outer cover is provided with support Plate, the both sides of outer cover are open, and its side is provided with pusher, and opposite side is provided with stacking mechanism.Stacking mechanism includes one The piling platform parallel with shield base supporting plate, piling platform bottom is provided with telescoping drive mechanism and guiding mechanism.It is actual On, multiple delivery outlets are additionally provided with the inside of holster shell, not shown in figure, these delivery outlets can be set by chassis interior The output band put outside transmission respectively.
As shown in Fig. 2 in the central area of holster shell, the adjacent successively from inside to outside and one-level disk machine that is distributed with one heart Structure 1, secondary turntable mechanism 2 and three-level rotating disk mechanism 3.As shown in figure 3, every grade of rotating disk mechanism includes Moving plate 11 and price fixing respectively 12, wherein price fixing 12 is fixed on base or support, and gap is maintained between Moving plate 11 and price fixing 12, is used as glade plane space 13. The locating slot of sample box 26 is evenly distributed with Moving plate 11, the locating slot of sample box 26 is connected with glade plane space 13.
Moving plate 11 and price fixing 12 are installed by bearing or by the way that together with track installation, meeting Moving plate 11 can be in price fixing It can be rotated on 12.Each Moving plate 11 respectively with the drive connection of each output end of drive mechanism so as to keeping Moving plates 11 at different levels synchronous or It is asynchronous to rotate.For example, the up or down regional location in each internal layer of Moving plate 11 or internal layer is provided with gear ring, each gear ring respectively with phase Gear is answered to engage, each gear is respectively arranged in the rotating shaft of correspondence motor.Or, each gear ring is realized by gear train to be joined Dynamic, each Moving plate 11 of linkage can be synchronized or not synchronized low speed rotation.
Liang Ge three class controls mechanism 5, two three class controls are set between one-level rotating disk mechanism 1 and secondary turntable mechanism 2 Mechanism 5 is symmetrical.As shown in Figure 4 and Figure 8, three class control mechanism 5 is the Matching installation in the gap of Moving plate 11 and price fixing 12 There is baffle plate 91, downcast hole 15 is provided with the price fixing 12 immediately below baffle plate 91.Pacified simultaneously on the inside of frame by fixed seat Outer layer push-pull mechanism 95 equipped with radial arrangement, the outer layer push-and-pull boom end of outer layer push-pull mechanism 95 is consolidated with the end of baffle plate 91 It is scheduled on together, internal layer push-pull mechanism is installed in the inner side of the outer layer pull bar or outer layer, the internal layer of internal layer push-pull mechanism is pushed away Tie rod end, which is provided with, cuts somebody's hair, and cuts somebody's hair positioned at the upside of Moving plate 11 and positioned at the positive upside of slide plate.In fact, three class control mechanism 5 is led To be located at Moving plate 11 and the region of price fixing 12 of one-level rotating disk mechanism 1, the sample box 26 from one-level rotating disk mechanism 1, according to micro- The activity ratio of biological detection or other necessary information results carry out whether automatic decision continues cycling through or enter secondary turntable Mechanism 2 is carried out cultivating and detecting again with friction speed and incubation time, also or directly exported by downcast hole 15.
Three Two-stage control mechanisms 6, three Two-stage controls are set between secondary turntable mechanism 2 and three-level rotating disk mechanism 3 Mechanism 6 is uniformly distributed.As shown in Figure 5 and Figure 8, Two-stage control mechanism 6 is also with the gap of price fixing 12 to match peace in Moving plate 11 Equipped with baffle plate 91, downcast hole 15 is provided with the price fixing 12 immediately below baffle plate 91.Pass through fixed seat on the inside of frame simultaneously The outer layer push-pull mechanism 95 of radial arrangement, the outer layer push-and-pull boom end of outer layer push-pull mechanism 95 and the end of baffle plate 91 are installed It is fixed together, internal layer push-pull mechanism, the internal layer of internal layer push-pull mechanism is installed in the inner side of the outer layer pull bar or outer layer Push-and-pull boom end, which is provided with, cuts somebody's hair, and cuts somebody's hair positioned at the upside of Moving plate 11 and positioned at the positive upside of slide plate.It is provided with the side respectively cut somebody's hair Barcode scanning head.Two-stage control mechanism 6 is predominantly located at Moving plate 11 and the region of price fixing 12 of one-level rotating disk mechanism 1, from secondary turntable machine The sample box 26 of structure 2, carries out whether automatic decision enters according to the activity ratio of microorganism detection or other necessary information results Enter three-level rotating disk mechanism 3 to carry out cultivating and detecting again with friction speed and incubation time, also or directly pass through downcast hole 15 Output.
Four one-level controlling organizations 7 are set on three-level rotating disk mechanism 3, are uniformly distributed during four one-level controlling organizations 7.Such as Shown in Fig. 6, a kind of form of one-level controlling organization 7 is that radially mounted have push-and-pull in the upside of Moving plate 11 of three-level rotating disk mechanism 3 Mechanism, the push-and-pull boom end of the push-pull mechanism, which is provided with, cuts somebody's hair, and is provided with barcode scanning head in the side respectively cut somebody's hair, cuts somebody's hair positioned at Moving plate 11 upsides.Or, another form of one-level controlling organization 7 as shown in Figure 7 is set on the price fixing 12 of three-level rotating disk mechanism 3 There is downcast hole 15, barcode scanning head is provided with above the side of downcast hole 15.With the corresponding holster shell of each one-level controlling organization 7 Discharging opening is provided with position;The lower end of feeding-passage 4 is located at the upside of the one-level rotating disk mechanism 1, the upper end of feeding-passage 4 Charging aperture is located at the upper end of holster shell;The rotation of the three class control mechanism 5, Two-stage control mechanism 6 and one-level controlling organization 7 The upper region in direction is provided with microorganism sample testing agency 8.
Detect that sample enters one-level rotating disk mechanism 1 from input channel in order respectively, the rotating speed of one-level rotating disk mechanism 1 is slower, Sample positioned at the top of one-level rotating disk mechanism 1 is cultivated or recovered by certain time first, enters detect for the first time afterwards, according to Whether testing result divides and is directly exported from downcast hole 15, or continues on one-level rotating disk mechanism 1 to cultivate at a slow speed, or enters Enter secondary turntable mechanism 2.Finally, divide from the delivery outlets of downcast hole 15 at different levels or from the delivery outlet output difference of holster shell side wall The sample box 26 of class.Barcode scanning mechanism believes detection information and mark according to the markup information of each sample box 26 of barcode scanning head collection Breath is conveyed to controller and is analyzed, calculated and stored in the lump.Necessary operation button can be arranged as required in holster shell And display, for the condition of culture and incubation time and the parameter of screening conditions in equipment to be inputted and changed.Or Directly relevant criterion is configured by background control center.Controller controls each drive mechanism and each push-pull mechanism after setting Run in operation sequence.

Claims (3)

1. a kind of microorganism continuous culture detects automatic splinter screening device for screening, including rotating mechanism, drive mechanism and testing agency, and Charging aperture and discharging opening, be additionally provided with base and closing holster shell, be provided with the holster shell temperature control system and Air distribution system and humidity control system;It is characterized in that:Rotating mechanism include it is adjacent successively from inside to outside and be distributed with one heart one Level rotating disk mechanism, secondary turntable mechanism and three-level rotating disk mechanism, every grade of rotating disk mechanism include Moving plate and price fixing, the price fixing respectively It is fixed on base or support, sample box locating slot is evenly distributed with the Moving plate, gap is maintained between Moving plate and price fixing, Sample box locating slot is connected with gap;Moving plate and price fixing are installed by bearing or by together with track installation, meeting Moving plate It is able to can be rotated on price fixing;Each output end drive connection of each Moving plate respectively with drive mechanism is so as to keep Moving plates at different levels same Step or asynchronous rotation;It is multiple at least provided with a three class control mechanism between one-level rotating disk mechanism and secondary turntable mechanism It is uniformly distributed during three class control mechanism;At least provided with a Two-stage control machine between secondary turntable mechanism and three-level rotating disk mechanism Structure, is uniformly distributed during multiple Two-stage control mechanisms;The three class control mechanism and Two-stage control mechanism are all in Moving plate and price fixing Gap in Matching installation have baffle plate, be provided with downcast hole on the price fixing immediately below baffle plate;Pass through simultaneously on the inside of frame Fixed seat is provided with the outer layer push-pull mechanism of radial arrangement, the outer layer push-and-pull boom end of outer layer push-pull mechanism and the baffle plate end It is fixed together, internal layer push-pull mechanism, the internal layer of internal layer push-pull mechanism is installed in the inner side of the outer layer pull bar or outer layer Push-and-pull boom end, which is provided with, cuts somebody's hair, and cuts somebody's hair on the upside of Moving plate and positioned at the positive upside of slide plate;At least set on three-level rotating disk mechanism An one-level controlling organization is put, is uniformly distributed during multiple one-level controlling organizations;The one-level controlling organization is in three-level disk machine Radially mounted on the upside of the Moving plate of structure to have push-pull mechanism, the push-and-pull boom end of the push-pull mechanism, which is provided with, cuts somebody's hair, and is respectively pushed away described The side of head is provided with barcode scanning head, cuts somebody's hair on the upside of Moving plate;Or the one-level controlling organization is in three-level rotating disk mechanism Downcast hole is provided with price fixing, barcode scanning head is provided with above downcast hole side;Machine corresponding with each one-level controlling organization Rack enclosure is provided with discharging opening at position;The lower end of feeding-passage is located on the upside of the one-level rotating disk mechanism, feeding-passage The charging aperture at end is located at the upper end of holster shell;The rotation of the three class control mechanism, Two-stage control mechanism and one-level controlling organization The upper region for turning direction is provided with microorganism sample testing agency.
2. microorganism continuous culture according to claim 1 detects automatic splinter screening device for screening, it is characterized in that:In holster shell Discharging opening is provided with lateral wall and outer cover is set, the bottom of the outer cover is provided with supporting plate, and the both sides of outer cover are open, one Side is provided with pusher, and opposite side is provided with stacking mechanism.
3. microorganism continuous culture according to claim 2 detects automatic splinter screening device for screening, it is characterized in that:Stacking mechanism includes One piling platform parallel with shield base supporting plate, piling platform bottom is provided with telescoping drive mechanism and guiding mechanism.
CN201710310028.9A 2017-05-05 2017-05-05 Automatically continuously culture detects screening plant to microorganism Pending CN107043696A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110066724A (en) * 2019-06-04 2019-07-30 天津市恒奥科技发展有限公司 Microculture real-time monitoring device and detection method

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