CN107027824A - A kind of banana blight bacteria Synergistic composition containing Chinese medical extract and agricultural chemicals - Google Patents
A kind of banana blight bacteria Synergistic composition containing Chinese medical extract and agricultural chemicals Download PDFInfo
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- CN107027824A CN107027824A CN201710438013.0A CN201710438013A CN107027824A CN 107027824 A CN107027824 A CN 107027824A CN 201710438013 A CN201710438013 A CN 201710438013A CN 107027824 A CN107027824 A CN 107027824A
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- agricultural chemicals
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- banana blight
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/26—Oxidation products of dithiocarbamic acid derivatives, e.g. thiuram sulfides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of banana blight bacteria Synergistic composition containing Chinese medical extract and agricultural chemicals, and the composition includes component A and B component；The component A includes Flos Caryophylli extract and coptis extract；The B component is made up of 50% Prochloraz wettable powder, 50% carbendazol wettable powder and 50% thiram wettable powder.The present invention has synergistic function by various ingredients combination, belongs to fungicidal agricultural chemicals, available for banana blight preventing and treating.
Description
Technical field
The invention belongs to technical field of plant protection, and in particular to a kind of banana blight containing Chinese medical extract and agricultural chemicals
Bacterium Synergistic composition.
Background technology
Banana blight is found in Australia first in the later stage eighties 19th century, and 1950s is in Panama
Pandemic.Since 1990s, banana blight has spread to each main banana production state in the whole world, as most
One of destructive banana disease.China or even global banana industry just meet with the Fusarium oxysporum of most serious since the dawn of human civilization so far
The Cuba specialized form Fusarium oxysporum f.sp. Cubense threat and challenge of infecting caused banana blight.
Banana blight can be by inhaling bud, soil, rain in spite of illness
The Medium Propagations such as water, irrigation water, air-flow and farm implements, and Fusarium oxysporum can long term survival in soil, general medicament
It is difficult to eradicate.In recent years up to more than 90% when China any of several broadleaf plants garden banana blight incidence of disease is serious, serious obstruction China banana industry
Development.
The practical efficiency of chemical pesticide is generally 10%, most all to remain in the environment.Chemical pesticide once enters
Environment, its toxicity, high residue characteristic will generating effect, the pollution of serious air, water body and soil is caused, so as to the mankind
Health care belt endangers.Current research in the preventing and treating of harmful organism it has been shown that repeating and uniquely applying individually activity
Component cause in many cases oneself through the active component is produced those harmful organisms of tolerance that is natural or adapting to every
The quick selection peeled off.It is current to be generally used to the mixture of different activities component prevent and treat in order to reduce tolerance risk of selection.
Bioactive substance or directly exploitation botanical pesticide are found in plant turns into one of the focus in current pesticide research field.
Wherein, Meng Zhaoli etc. isolates the compound for having very high activity to phytopathogen from ginkgo, through Structural Identification, manual simulation
Synthesis, successfully develops " green Supreme Being " agricultural bactericidal agent series products；Rhizoma Acori Graminei extract is withered to botrytis cinerea and muskmelon
Germ；Small rose extract reaches 100% to botrytis cinerea mycelial growth inhibition rate.Therefore, during searching one kind is included
The banana blight bacteria Synergistic combination of medicament extract and agricultural chemicals, the efficiently preventing and treating for banana blight bacteria is necessary.
The content of the invention
To solve to make up the deficiencies in the prior art, there is provided a kind of banana blight bacteria collaboration containing Chinese medical extract and agricultural chemicals
Synergistic composition.
The technical scheme is that:
A kind of banana blight bacteria Synergistic composition comprising Chinese medical extract and agricultural chemicals, including component A and B component, institute
State component A to be made up of cloves and coptis extract, the B component is by 50% Prochloraz wettable powder, 50% carbendazim wettable
Pulvis and 50% thiram wettable powder（Mass ratio 1:5:10-7:3:2）Composition, wherein component A and component B are with 1:5-1:8
Gross weight ratio is present.
The weight ratio of Flos Caryophylli extract and coptis extract is in component A：（1-10）:（1-7）.
Cloves and coptis extract are prepared by a conventional method, and unit is 1g/mL.
The preparation method of the composition, comprises the following steps：The cloves of the component A and coptis extract, are with logical
The ethanol immersion effective component extracting extract solution of mass fraction 75% is crossed, the extract solution is well mixed with the B component, is made
Into Chinese medicine and the synergistic composition of agricultural chemicals.
The beneficial effects of the invention are as follows:
The present invention has synergistic function by various ingredients combination, belongs to fungicidal agricultural chemicals, anti-available for banana blight
Control.
Embodiment
The principle and feature to the present invention are described with reference to embodiments, and the given examples are served only to explain the present invention,
It is not intended to limit the scope of the present invention.
Embodiment 1
A kind of banana blight bacteria Synergistic composition comprising Chinese medical extract and agricultural chemicals, including component A and B component, institute
Component A is stated by cloves and the mL of coptis extract 2, the weight ratio of Flos Caryophylli extract and coptis extract is：5:4, the B component
It is made up of 50% Prochloraz wettable powder 1g, 50% carbendazol wettable powder 5g and 50% thiram wettable powder 10g；Its
Described in component A and B component weight ratio be 1：8.
A kind of preparation method of banana blight bacteria Synergistic composition comprising Chinese medical extract and agricultural chemicals, bag
Include following steps：Each component passes through mass fraction 75% according to above-mentioned weight weighing, the component A cloves and coptis extract
Ethanol immersion effective component extracting extract solution, the extract solution is mixed with the B component, and the synergy of Chinese medicine and agricultural chemicals is made
Composition.
Embodiment 2
A kind of banana blight bacteria Synergistic composition comprising Chinese medical extract and agricultural chemicals, including component A and B component, institute
Component A is stated by cloves and the mL of coptis extract 2.4, the weight ratio of Flos Caryophylli extract and coptis extract is：1:1.
The B component is wettable by 50% Prochloraz wettable powder 7g, 50% carbendazol wettable powder 3g and 50% thiram
Property pulvis 2g composition；Wherein described component A and B component weight ratio is 1：5.
A kind of preparation method of banana blight bacteria Synergistic composition comprising Chinese medical extract and agricultural chemicals, bag
Include following steps：Each component passes through mass fraction according to above-mentioned weight weighing, the component A cloves and coptis extract
75% ethanol immersion effective component extracting, the extract solution is mixed with the B component, and the synergy group of Chinese medicine and agricultural chemicals is made
Compound.
Embodiment 3
From the composition in embodiment 1 or 2, its inhibitory action to banana blight bacteria growth rate is detected.In ultra-clean work
Make on platform, banana blight FocWT sequencing bacterium are inoculated into PDA liquid medium first, at a temperature of 28 DEG C, dark culturing 3-
5 days.On superclean bench, the banana blight bacteria (the small bacterium dish of 0.5cm) of culture is inoculated into PDA solid mediums（Contain
Composition in the embodiment 1 or 2 of 1000 times of dilutions, and A respectively and B component in Examples 1 and 2）, at a temperature of 28 DEG C,
Dark culturing measures colony diameter after 3-5 days, to be not added with the PDA culture medium of composition as control.Growth rate statistics discovery,
Under composition, the A of embodiment 1 and B component, the A of embodiment 2 and B component effect in embodiment 1 or 2, banana blight bacteria life
Long speed inhibition is respectively up to 80.3%, 90.8%, 50.3%, 68.4%, 59.6% and 60.4%.It these results suggest that：Embodiment 1
Or the composition inhibition in 2 is apparently higher than the inhibition of component.
Embodiment 4
From the composition in embodiment 1 or 2, its inhibitory action colonized to banana blight bacteria is detected.In superclean bench
On, Fujian separation banana blight Foc37 bacterium are inoculated on PDA solid mediums first, at a temperature of 28 DEG C, dark culturing 3-
5 days.Then by inoculation into PDA liquid medium, formed greatly with nutrient solution after shaking table shaken cultivation 150rpm, 3-5d
Measure small-sized conidiospore.Composition, the A of embodiment 1 and B component, the A of embodiment 2 and B component difference in embodiment 1 or 2
Pouring root after 1000 times of sterilized water dilutions, 1.0 × 10 are diluted to after 1 day by pathogen spore suspension5, then it is inoculated with 3-4 piece true leaves
Banana Seedlings, with pour apply PDA liquid medium control, it is 50 mL that every plant, which is poured the amount of applying,.Count and find after 40 days, implementing
Under composition effect in example 1 or 2, banana blight inhibition is obvious, respectively up to 88.4% and 89.7%.And in embodiment 1
A and B component, the A of embodiment 2 and B component effect under, banana blight inhibition only reaches 53.1% respectively, 72.3%,
58.4% and 68.7%.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (5)
1. a kind of banana blight bacteria Synergistic composition containing Chinese medical extract and agricultural chemicals, it is characterised in that：The combination
Thing includes component A and B component；The component A includes Flos Caryophylli extract and coptis extract；The B component can by 50% Prochloraz
WP, 50% carbendazol wettable powder and 50% thiram wettable powder composition.
2. a kind of banana blight bacteria Synergistic composition containing Chinese medical extract and agricultural chemicals according to claim 1,
It is characterized in that：Wherein component A and component B weight ratio are 1:（5-8）.
3. a kind of banana blight bacteria Synergistic composition containing Chinese medical extract and agricultural chemicals according to claim 1,
It is characterized in that：The weight ratio of Flos Caryophylli extract and coptis extract is in component A：（1-10）:（1-7）.
4. a kind of banana blight bacteria Synergistic composition containing Chinese medical extract and agricultural chemicals according to claim 1,
It is characterized in that：B component is by 50% Prochloraz wettable powder, 50% carbendazol wettable powder and 50% thiram wettable powder
Agent in mass ratio 1:5:10-7:3:2 mixing.
5. a kind of prepare a kind of banana blight bacteria Synergistic group containing Chinese medical extract and agricultural chemicals as claimed in claim 1
The method of compound, it is characterised in that：Comprise the following steps：
（1）The preparation of component A：
Component A extracts active ingredients liquid is extracted by ethanol immersion；
（2）By step（1）Extract solution is well mixed with B component, and a kind of banana comprising Chinese medical extract and agricultural chemicals is made and withers
Germ Synergistic composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710438013.0A CN107027824B (en) | 2017-06-12 | 2017-06-12 | A kind of banana blight bacteria synergy composition containing Chinese medical extract and pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710438013.0A CN107027824B (en) | 2017-06-12 | 2017-06-12 | A kind of banana blight bacteria synergy composition containing Chinese medical extract and pesticide |
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| Publication Number | Publication Date |
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| CN107027824A true CN107027824A (en) | 2017-08-11 |
| CN107027824B CN107027824B (en) | 2019-09-10 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111004061A (en) * | 2019-11-30 | 2020-04-14 | 广西金穗农业集团有限公司 | Anti-wilt agent applied to banana fertilizer and preparation method thereof |
| CN115039797A (en) * | 2022-03-24 | 2022-09-13 | 福建省农业科学院亚热带农业研究所（福建省农业科学院蔗麻研究中心） | Clove essential oil antibacterial mixed solution and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111004061A (en) * | 2019-11-30 | 2020-04-14 | 广西金穗农业集团有限公司 | Anti-wilt agent applied to banana fertilizer and preparation method thereof |
| CN115039797A (en) * | 2022-03-24 | 2022-09-13 | 福建省农业科学院亚热带农业研究所（福建省农业科学院蔗麻研究中心） | Clove essential oil antibacterial mixed solution and application thereof |
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| Publication number | Publication date |
|---|---|
| CN107027824B (en) | 2019-09-10 |
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