CN107027519A - The mycorrhiza fungi seeding cultivating method of Panzhihua ferfas - Google Patents
The mycorrhiza fungi seeding cultivating method of Panzhihua ferfas Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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Abstract
The invention belongs to technical field of plant cultivation, and in particular to a kind of bacterium mycorrhiza fungi seeding cultivating method of Panzhihua ferfas.Lack the problem of Panzhihua ferfas carries out the method for mycorrhiza fungi seeding cultivating for prior art, the present invention provides a kind of mycorrhiza fungi seeding cultivating method of Panzhihua ferfas, comprised the following steps：The collection and preservation of Panzhihua ferfas strain, the cultivation with the Va Mycorrhiza Seedling after when preparation method, pecan tree seed collection and preservation, the cultivation of pecan tree aseptic seedling, inoculation, inoculation Panzhihua ferfas of Panzhihua ferfas microbial inoculum.Present invention exploitation pecan tree is used for the inoculation of Panzhihua ferfas, compensate for the vacancy that areas of higher temperature cultivates truffle mycorrhiza fungi seeding economic fruit；Constituted and matched using distinctive ferfas microbial inoculum so that mycorhiza infection rate is more than 85% after microbial inoculum inoculation, greatly improves plant percent.The inventive method is simple to operate, and infection rate is high, survival rate height is transplanted, with significant economic benefit.
Description
Technical field
The invention belongs to technical field of plant cultivation, and in particular to a kind of mycorrhiza fungi seeding cultivating method of Panzhihua ferfas.
Background technology
Ferfas (T.ruffles) is ascomycetes Tuberale (Tuberales) Tuberaceae (Tuberaceae) Tuber
(Tuber) it is, symbiosis edible mushroom most precious in the world, it is international because of its limits throughput with peculiar fragrance and nutritive value
Market at prices is expensive, and supply falls short of demand, is described as " black diamond ".
Panzhihua ferfas (T.panzhihuanense) fructification is very large, single heavy up to more than 450g, there is fragrant, meat
Matter is fine and smooth, good in taste, is the white blocks of the domestic great commercial value found till now and larger business development potentiality
Bacterium new varieties.More than 3cm Panzhihua ferfas internal price is in 1500 yuan of kg~1~2 000 yuan of kg~1, far above print
Spend ferfas (500 yuan of kg~1~800 yuan of kg~1).But Panzhihua ferfas Yield comparison is limited, supply falls short of demand in market.
In order to improve the yield of Panzhihua ferfas, existing resource is effectively protected, Panzhihua ferfas sustainable utilization is realized,
Can be by carrying out Panzhihua ferfas artificial cultivation.It is with high content of technology but Panzhihua ferfas artificial cultivation is relatively difficult.Climb branch
The key of flower ferfas artificial cultivation is the cultivation of Panzhihua truffle mycorrhiza fungi seeding, and ferfas growth must be with suitable host plant root
Battalion of system commensalism, otherwise will be unable to form ascocarp, and this means that artificial cultivation ferfas be unable to do without live body host plant.Block
The successful key of bacterium artificial cultivation is to first have to being inoculated into ferfas into suitable host plant root, and ferfas mycelium germination simultaneously infects
Root system of plant, forms symbiosis with plant, obtains Va Mycorrhiza Seedling.Again by Va Mycorrhiza Seedling plantation into suitable environment, the bacterium of ferfas
Silk can grow with plant-root growth, and after the mycorhiza of ferfas grows to certain amount, the extension mycelia on mycorhiza surface will be turned round
Tie and start fruiting.From the time for being inoculated into fruiting it is generally necessary to 5~8 years, it can be harvested every year once after fruiting, it is sustainable to receive
Obtain 30~50 years.
Panzhihua ferfas is the white ferfas novel species of most commercial promise, and there is presently no ripe Panzhihua ferfas people
Work cultural method.In existing India truffle and summer truffle mycorrhiza fungi seeding cultivating method, generally there are solid fungicide and liquid bacterial agent to enter
Row inoculating two kinds method, solid fungicide inoculation typically first cultivates sterile bare-root seeding, and being changed during inoculation has one section after nutritive cube, inoculation
The seedling-slowing stage of time, this can cause the inoculation seedling death rate high, influence seedling growth, delay mycorhiza to be formed.Liquid bacterial agent inoculation side
Method inoculum concentration is uneven, ferfas spore have it is many, have few, the quality that mycorhiza is formed after inoculation is uneven, or even part nursery stock is not
It can infect and form mycorhiza.
Pecan tree [Carya illinoensis (Wangenn.) K.Koch], originates in the U.S., is four big hard in the world
One of fruit.Pecan tree shell is thin, takes benevolence easily, kernel percent 50% -70%, oil content 70~80%, and unrighted acid contains
Amount 60% or so, undersaturated aliphatic acid and high to the beneficial various amino acid ratio olives of human body.4 after pecan tree definite value
Start to bear fruit within~5 years, 10 years start to abound with, and abound with the phase 50~70 years, 100-250kg/ mus, 6~8 dollars on international market/
Kg, domestic 60~120 yuan/kg of supermarket, are 4~6 times of common walnut, are only calculated according to minimum output and lowest price, per mu yield
Value can reach 6000 yuan.
Pecan tree is in 15-20 DEG C of samming of year, 800~1600mm of rainfall, 1 5-10 DEG C of monthly average temperature, 7 monthly average temperature
20~30 DEG C, 220~330 days frost-free periods, the area of P in soil H values 6~8 can be planted, and be a kind of unique suitable low altitude area cultivation
Walnut Cultivars, low altitude area region is adapted to pecan tree cultivation in Panzhihua.Ferfas is 1100~2600m's of Panzhihua height above sea level
Scope is distributed, according to the principle of " the suitable suitable bacterium of tree ", to realize the maximization of pecan tree benefit, it is necessary to carry out Panzhihua
Ferfas is inoculated with the breeding method of pecan tree Va Mycorrhiza Seedling.
The content of the invention
The technical problem to be solved in the present invention is：Prior art lacks the method for Panzhihua ferfas progress mycorrhiza fungi seeding cultivating
Problem.
The present invention solve technical problem technical scheme be：A kind of mycorrhiza fungi seeding cultivating method of Panzhihua ferfas is provided.Should
Method comprises the following steps：
A, Panzhihua ferfas strain collection and preservation
Panzhihua ferfas mature sporophore is collected, powder is ground into after sterilization；
B, Panzhihua ferfas microbial inoculum with when preparation method
The powder that step a is prepared and agstone, vermiculite power, turf powder, EM saccharomycete and bacillus subtilis
Matched, be well mixed by weight the ﹕ 1~2 of 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕ 1~2；
C, pecan tree seed collection and preservation
Pecan tree seed is collected, 5~7d is soaked after sterilization；
D, pecan tree aseptic seedling cultivation
Hickory nut seed after immersion is seeded into the seedlings nursing plate equipped with sterilization matrix, addition 2~3cm sterilization matrix is covered
Lid seed, pours permeable, is placed in greenhouse and cultivates；
E, inoculation
After step d pecan tree culture is carried out 3 months, the step b microbial inoculums prepared are inoculated with seedlings nursing plate, often
Strain aseptic seedling inoculation ferfas 5~8g of microbial inoculum, then 2~3cm sterilization matrix is covered, pour permeable；
F, Arbuscular Mycorrhizal Fungi seedling cultivation
The hickory nut plant of the inoculated ferfas of step e is cultivated 4~6 months in greenhouse, Panzhihua truffle mycorrhiza fungi is obtained
Seedling.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the concrete operations sterilized described in step a are to use 70
~75% 3~5min of alcohol or hydrogen peroxide dipping.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, diameier≤0.5mm described in step a.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, limestone powder, vermiculite power described in step b,
The granularity of turf powder is≤0.5mm.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the processing mode sterilized described in step c is：With
0.3~0.5% 3~5h of potassium permanganate liquid immersion treatment.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, immersion described in step c is clear water immersion, daily
Change water 1~2 time.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, sterilization matrix described in step d is by weight grass
Charcoal soil：Vermiculite：Perlite：Agstone=2~﹕ 1~1.5 of 3 ﹕, 1~2 ﹕ 1~2 are mixed.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization process of sterilization matrix is described in step d
Sterilized 2~3 hours at 121~126 DEG C.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization matrix loaded described in step d accounts for nursery
The 1/2~2/3 of disk depth.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, fallen described in step d in seedlings nursing plate equipped with 4~8
Root line.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the condition of culture described in step d, e is：Matrix
Humidity 25~35%, 20~30 DEG C of greenhouse temperature, greenhouse humidity 60~80%.
Beneficial effects of the present invention are：The present invention provides the mycorrhiza fungi seeding cultivating that a kind of Panzhihua ferfas is inoculated with pecan tree
Method, the inoculation that pecan tree is used for Panzhihua ferfas compensate for areas of higher temperature cultivation economic fruit truffle mycorrhiza fungi seeding
Vacancy；The distinctive microbial inoculum composition of the present invention and proportioning so that microbial inoculum inoculation postoperative infection rate is up to 90%, is cultivated using seedlings nursing plate,
The line of falling root is housed so that fibrous root is more and growth distribution is uniform, and mycorhiza quantity is more, and disk is not moved after inoculation, greatly improves nursery stock in disk
Survival rate, and without seedling-slowing stage.The inventive method is simple to operate, and infection rate is high, transplants survival rate height, with significant economic effect
Benefit.
Figure of description
Accompanying drawing 1 show nonvaccinated pecan tree root；
Accompanying drawing 2 show the pecan tree mycorhiza after embodiment 1 is inoculated with；
Accompanying drawing 3 show the extension mycelia on Va Mycorrhiza Seedling after embodiment 1 is inoculated with；
Accompanying drawing 4 show the Hartig net knot of mycorhiza top layer horse-hof shape after embodiment 1 is inoculated with.
Embodiment
The invention provides a kind of mycorrhiza fungi seeding cultivating method of Panzhihua ferfas, comprise the following steps：
A, Panzhihua ferfas strain collection and preservation
Panzhihua ferfas mature sporophore is collected, with 70~75% 3~5min of alcohol or hydrogen peroxide dipping, is ground into straight
Footpath≤0.5mm powder；
B, Panzhihua ferfas microbial inoculum with when preparation method
The powder that step a is prepared and agstone, vermiculite power, turf powder, EM saccharomycete and bacillus subtilis
Matched, be well mixed by weight the ﹕ 1~2 of 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕ 1~2；
C, pecan tree seed collection and preservation
Pecan tree seed is collected, 5~7d is soaked after sterilization；
D, pecan tree aseptic seedling cultivation
Hickory nut seed after immersion is seeded into the seedlings nursing plate equipped with sterilization matrix, addition 2~3cm sterilization matrix is covered
Lid seed, pours permeable, is placed in greenhouse and cultivates；
E, inoculation
After step d pecan tree culture is carried out 3 months, the step b microbial inoculums prepared are inoculated with seedlings nursing plate, often
Strain aseptic seedling inoculation ferfas 5~8g of microbial inoculum, then 2~3cm sterilization matrix is covered, pour permeable；
F, Arbuscular Mycorrhizal Fungi seedling cultivation
The hickory nut plant of the inoculated ferfas of step e is cultivated 4~6 months in greenhouse, Panzhihua truffle mycorrhiza fungi is obtained
Seedling.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, limestone powder, vermiculite power described in step b,
The granularity of turf powder is≤0.5mm.
Present invention selection limestone powder, vermiculite power, turf powder and EM saccharomycete and Panzhihua ferfas use cooperatively, lime
Stone powder can make strain keep drying, moreover it is possible to provide the calcareous of ferfas growth, adjust pH value；Vermiculite power can maintain microbial inoculum
Gas porosity, plays filling effect；Turf contains humic acid, can promote ferfas spore germination, promotes plant root growth；EM yeast
Bacterium and bacillus subtilis are conducive to the synthesis of truffle mycorrhiza fungi, can adjust the balance of Soil Microorganism group, improve ferfas bacterium
Root infection rate；Above-mentioned raw materials are mixed by weight the ﹕ 1~2 of 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕ 1~2, can be with
Mycorhiza infection rate is set to reach more than 90%, the survival rate more than 98% of nursery stock goes out shoot survival percent high.The such ratio strain of selection
Holding time is longer, and the germination rate of strain miospore is high, easily infects nursery stock and forms mycorhiza.
Described limestone powder, vermiculite power, the granularity of turf powder are preferably≤0.5mm, can make spore preferably from
Ascus separate out, is easier to be uniformly dispersed in the root of sapling in inoculation.It is 30~35 ﹕ 8 by the limited proportion of three
~12 ﹕ 20~25, can make the holding time of microbial inoculum more long, and more loose ventilative, cohesive is good, and inoculation postoperative infection rate is high.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the processing mode sterilized described in step c is：With
0.3~0.5% 3~5h of potassium permanganate liquid immersion treatment.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, immersion described in step c is clear water immersion, daily
Change water once.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, sterilization matrix described in step d is by weight grass
Tan Tu ﹕ Zhi Shi ﹕ Zhen Zhu Yan ﹕ agstone=2~﹕ 1~1.5 of 3 ﹕, 1~2 ﹕ 1~2 are mixed.Turf contains humic acid, can
Promote ferfas spore germination, promote plant root growth；Vermiculite can maintain the gas porosity of microbial inoculum, and there is provided the various of plant growth
Mineral matter；Perlite plays filling effect, loose matrix, increase the permeable of matrix, gas permeability and water-retaining property；Agstone can be provided
Calcareous, the regulation matrix pH value of ferfas growth.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization process of sterilization matrix is described in step d
Sterilized 2~3 hours at 121~126 DEG C.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization matrix loaded described in step d accounts for nursery
The 1/2~2/3 of disk depth.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, fallen described in step d in seedlings nursing plate equipped with 4~8
Root line.On the one hand the line of falling root can promote fibrous root homoepitaxial, make fibrous root quantity many, and be evenly distributed, and on the other hand, can make
Root radially grows, and will not spiral, and transplanting survival rate is high.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the condition of culture described in step d, e is：Matrix
Humidity 25~35%, 20~30 DEG C of greenhouse temperature, greenhouse humidity 60~80%.
Present invention selection pecan tree carries out the cultivation of Va Mycorrhiza Seedling, and hickory nut is a kind of economic fruit, suitably in temperature
Higher ecological region planting, compared to economic tree Chinese chestnut, fibert of existing inoculation ferfas etc., adapts to hot environment, and have
Beneficial to the mycorhiza inoculation of Panzhihua ferfas, rate of vaccination is high, improves the benefit of ferfas plantation, has filled up areas of higher temperature cultivation
The vacancy of economic fruit truffle mycorrhiza fungi seeding.
Breeding method of the present invention understands, simply, and cost is low, and inoculation ferfas infection is fast, and mycorhiza infection rate is high, no slow seedling
Phase, no dead seedling, truffle mycorrhiza fungi seeding growth is neat.For realize Panzhihua ferfas artificial cultivation established solid foundation there is provided
The safeguards technique of high-quality Va Mycorrhiza Seedling, Panzhihua ferfas artificial cultivation success not only can effectively protect existing Panzhihua ferfas to provide
Source, also meets the material life demand of the growing high-quality of the mankind, with wide market prospects.
Explanation is further explained to the embodiment of the present invention below by embodiment, but does not indicate that and sends out this
Bright protection domain is limited in described in embodiment in scope.
EM saccharomycete in the embodiment of the present invention is purchased to Shanxi Chang Tai animal pharmaceutical estates Co., Ltd, and execution standard is Q/
SCY010-213；Bacillus subtilis is purchased to Wuhan Nature, and product standard card number is Q/WTH05-
2012；
Panzhihua ferfas is purchased to Panzhihua Yi ferfas Co., Ltd.
Embodiment 1 carries out mycorrhiza fungi seeding cultivating with the inventive method to Panzhihua ferfas
Panzhihua truffle mycorrhiza fungi seeding cultivating, concrete operation step is as follows：
A, Panzhihua ferfas strain collection and preservation
Collect Panzhihua ferfas mature sporophore, after 70% alcohol or hydrogen peroxide dipping 3min, be ground into diameter≤
0.5mm powder；
B, Panzhihua ferfas microbial inoculum with when preparation method
The ferfas powder that step a is prepared and limestone powder, vermiculite power, turf powder, EM saccharomycete and withered grass bud
Spore bacillus is matched by weight the ﹕ 1 of 30 ﹕, 8 ﹕, 20 ﹕, 10 ﹕ 1, is well mixed；Described limestone powder, vermiculite power, turf powder
Granularity be≤0.5mm；
C, pecan tree seed collection and preservation
High-quality pecan tree seed is collected, is carried out disinfection, is used after sterilization with 0.3% potassium permanganate liquid immersion treatment 3h
Clear water soaks 5d, and holding changes water daily；
D, pecan tree aseptic seedling cultivation
1/2 sterilization matrix will be loaded in seedlings nursing plate with 8 lines of falling root, the sterilization matrix compares turf by weight
Soil：Vermiculite：Perlite：Agstone=﹕ 1 of 2 ﹕, 1 ﹕ 1 are mixed, and are sterilized 2 hours at 121 DEG C after mixing, inoculation step c's
Hickory nut seed, then 2~3cm sterilization matrix covering seed is added, pour permeable, be placed in greenhouse and cultivate, matrix is kept during culture
Humidity 25%, 20 DEG C of greenhouse temperature, greenhouse humidity 60%；
E, inoculation
After step d is cultivated 3 months, the step b microbial inoculums prepared are inoculated with seedlings nursing plate, every plant of aseptic seedling is inoculated with ferfas
Microbial inoculum 4g, then 2~3cm sterilization matrix is covered, pour permeable；
F, Arbuscular Mycorrhizal Fungi seedling cultivation
The hickory nut plant of the inoculated ferfas of step e is cultivated in greenhouse, soil humidity 25%, greenhouse Air Temperature is kept
20 DEG C of degree, greenhouse air humidity 60% obtains Panzhihua truffle mycorrhiza fungi seeding after 4~6 months.
Embodiment 2 carries out mycorrhiza fungi seeding cultivating with the inventive method to Panzhihua ferfas
Panzhihua truffle mycorrhiza fungi seeding cultivating, concrete operation step is as follows：
A, Panzhihua ferfas strain collection and preservation
Collect Panzhihua ferfas mature sporophore, after 75% alcohol or hydrogen peroxide dipping 5min, be ground into diameter≤
0.5mm powder；
B, Panzhihua ferfas microbial inoculum with when preparation method
The ferfas powder that step a is prepared and limestone powder, vermiculite power, turf powder, EM saccharomycete and withered grass bud
Spore bacillus is matched by weight the ﹕ 1.5 of 33 ﹕, 10 ﹕, 22 ﹕, 12 ﹕ 1.5, is well mixed；Described limestone powder, vermiculite power, grass
The granularity of powdered carbon is≤0.5mm；
C, pecan tree seed collection and preservation
High-quality pecan tree seed is collected, is carried out disinfection, is used after sterilization with 0.5% potassium permanganate liquid immersion treatment 5h
Clear water soaks 7d, and holding changes water daily；
D, pecan tree aseptic seedling cultivation
2/3 sterilization matrix will be loaded in seedlings nursing plate with 8 lines of falling root, the sterilization matrix compares turf by weight
Soil：Vermiculite：Perlite：Agstone=﹕ 1.5 of 3 ﹕, 1 ﹕ 2 are mixed, and are sterilized 3 hours at 126 DEG C after mixing, inoculation step c
Hickory nut seed, then add 2~3cm sterilization matrix covering seed, pour permeable, be placed in greenhouse cultivate, base is kept during culture
Matter humidity 35%, 35 DEG C of greenhouse temperature, greenhouse humidity 80%；
E, inoculation
After step d is cultivated 3 months, the step b microbial inoculums prepared are inoculated with seedlings nursing plate, every plant of aseptic seedling is inoculated with ferfas
Microbial inoculum 5g, then 2~3cm sterilization matrix is covered, pour permeable；
F, the cultivation for being inoculated with Panzhihua truffle mycorrhiza fungi seeding
The hickory nut plant of the inoculated ferfas of step e is cultivated in greenhouse, soil humidity 30%, greenhouse Air Temperature is kept
30 DEG C of degree, greenhouse air humidity 80% obtains Panzhihua truffle mycorrhiza fungi seeding after 4~6 months.
Embodiment 3 carries out offspring cultivation with the inventive method to Panzhihua ferfas
Panzhihua block mycorrhiza fungi seeding cultivating, concrete operation step is as follows：
A, Panzhihua ferfas strain collection and preservation
Collect Panzhihua ferfas mature sporophore, after 75% alcohol or hydrogen peroxide dipping 5min, be ground into diameter≤
0.5mm powder；
B, Panzhihua ferfas microbial inoculum with when preparation method
The ferfas powder that step a is prepared and limestone powder, vermiculite power, turf powder, EM saccharomycete and withered grass bud
Spore bacillus is matched by weight the ﹕ 2 of 35 ﹕, 12 ﹕, 25 ﹕, 15 ﹕ 2, is well mixed；Described limestone powder, vermiculite power, turf powder
Granularity be≤0.5mm；
C, pecan tree seed collection and preservation
High-quality pecan tree seed is collected, is carried out disinfection, is used after sterilization with 0.5% potassium permanganate liquid immersion treatment 3h
Clear water soaks 5d, and holding changes water daily；
D, pecan tree aseptic seedling cultivation
1/2 sterilization matrix will be loaded in seedlings nursing plate with 8 lines of falling root, the sterilization matrix compares turf by weight
Soil：Vermiculite：Perlite：Agstone=﹕ 1 of 3 ﹕, 2 ﹕ 2 are mixed, and are sterilized 3 hours at 121 DEG C after mixing, inoculation step c's
Hickory nut seed, then 2~3cm sterilization matrix covering seed is added, pour permeable, be placed in greenhouse and cultivate, matrix is kept during culture
Humidity 35%, 35 DEG C of greenhouse temperature, greenhouse humidity 80%；
E, inoculation
After step d is cultivated 3 months, the step b microbial inoculums prepared are inoculated with seedlings nursing plate, every plant of aseptic seedling is inoculated with ferfas
Microbial inoculum 6g, then 2~3cm sterilization matrix is covered, pour permeable；
F, the cultivation for being inoculated with Panzhihua truffle mycorrhiza fungi seeding
The hickory nut plant of the inoculated ferfas of step e is cultivated in greenhouse, soil humidity 30%, greenhouse Air Temperature is kept
30 DEG C of degree, greenhouse air humidity 80% obtains Panzhihua truffle mycorrhiza fungi seeding after 4~6 months.
Comparative example 1 carries out truffle mycorrhiza fungi seeding cultivating without saccharomycete and bacillus subtilis
In comparative example 1, in addition in Panzhihua ferfas microbial inoculum proportioning without saccharomycete and bacillus subtilis, remaining operation
Be the same as Example 1.
Comparative example 2 is not provided with the line of falling root and carries out truffle mycorrhiza fungi seeding cultivating
In comparative example 2, in addition in the seedlings nursing plate described in step d without the line of falling root, remaining operation be the same as Example 1.
Comparative example 3 carries out truffle mycorrhiza fungi seeding cultivating using existing method
Comparative example 3 first cultivates aseptic seedling with seedlings nursing plate, aseptic seedling is taken out during inoculation, main root is cut off to use existing method,
The seedling medium of the first placement 2/3 in pot for growing seedlings, sterile bare-root seeding is put into pot for growing seedlings, is accessed ferfas microbial inoculum, is further filled with 1/3
Substrate soil.
Comparative example 4 carries out cultivating seedlings using existing method
The operating method of comparative example 4 is not in addition to ferfas microbial inoculum is accessed, and remaining operation is with comparative example 3.
The Va Mycorrhiza Seedling being inoculated with to embodiment and comparative example carried out one-time detection every 15 days, and survival rate is counted at 6 months, is surveyed
Mycorhiza infection rate, biomass are measured, result as shown in table 1 below is obtained.
The distinct methods of table 1 carry out the effect table of offspring cultivation
From experimental result：Using the inventive method, the truffle mycorrhiza fungi seeding formation time is short, can form ferfas bacterium within 45 days
Root, and infection rate is up to more than 85%, and nursery stock infects survival rate to more than 98%；The height of seedling of truffle mycorrhiza fungi seeding, ground diameter are all
Higher than existing method, root system is more and is evenly distributed, and quality is good, and the quality of Va Mycorrhiza Seedling is high.
Microexamination is carried out to the mycorhiza in embodiment 1, sampled using the methods of sampling of right-angled intersection, sampling intensity is
5%, count the synthesis situation and growing state of truffle mycorrhiza fungi.
From result：The pecan tree root for not being inoculated with Panzhihua ferfas is thinner, and tip is sharp (see Fig. 1)；Embodiment 1
Be inoculated with Panzhihua ferfas formed mycorhiza pecan tree root it is thicker, form is bar-shaped, feather fractures, mycorhiza initial stage, and end is in
Light yellow, color is gradually deepened in light salmon or light brown afterwards (see Fig. 2)；
Surface can be clearly apparent under the microscope a translucent extension mycelial structure, extension mycelia is upright, not bifurcated, have
Grow at mycorhiza surface to sequence (see Fig. 3)；Mycorhiza top layer forms the sheath cell institutional framework of the Hartig net layer of horse-hof shape (see figure
4)。
Claims (10)
1. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas, it is characterised in that comprise the following steps：
A, Panzhihua ferfas strain collection and preservation
Panzhihua ferfas mature sporophore is collected, powder is ground into after sterilization；
B, Panzhihua ferfas microbial inoculum with when preparation method
The powder that step a is prepared is with agstone, vermiculite power, turf powder, EM saccharomycete and bacillus subtilis by weight
Amount is matched than the ﹕ 1~2 of 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕ 1~2, is well mixed；
C, pecan tree seed collection and preservation
Pecan tree seed is collected, 5~7d is soaked after sterilization；
D, pecan tree aseptic seedling cultivation
Hickory nut seed after immersion is seeded into the seedlings nursing plate equipped with sterilization matrix, 2~3cm of addition sterilization matrix coverings kind
Son, pours permeable, is placed in greenhouse and cultivates；
E, inoculation
After step d pecan tree culture is carried out 3 months, the step b microbial inoculums prepared are inoculated with seedlings nursing plate, every plant of nothing
Bacterination ferfas 5~8g of microbial inoculum, then 2~3cm sterilization matrix is covered, pour permeable；
F, Arbuscular Mycorrhizal Fungi seedling cultivation
The hickory nut plant of the inoculated ferfas of step e is cultivated 4~6 months in greenhouse, Panzhihua truffle mycorrhiza fungi seeding is obtained.
2. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Disappear described in step a
The concrete operations of poison are 3~5min of alcohol or hydrogen peroxide dipping with 70~75%.
3. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Described in step a
Diameier≤0.5mm.
4. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Described in step b
The granularity of limestone powder, vermiculite power and turf powder is≤0.5mm.
5. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Disappear described in step c
Poison processing mode be：With 0.3~0.5% 3~5h of potassium permanganate liquid immersion treatment.
6. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Gone out described in step d
Bacterium matrix is by weight turfy soil：Vermiculite：Perlite：Agstone=2~﹕ 1~1.5 of 3 ﹕, 1~2 ﹕ 1~2 are mixed.
7. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Gone out described in step d
The sterilization process of bacterium matrix is to be sterilized 2~3 hours at 121~126 DEG C.
8. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Filled described in step d
The sterilization matrix entered accounts for the 1/2~2/3 of seedlings nursing plate depth.
9. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Educated described in step d
Equipped with 4~8 lines of falling root in seed plate.
10. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that：Described in step d, e
Condition of culture be：Soil humidity 25~35%, 20~30 DEG C of greenhouse temperature, greenhouse humidity 60~80%.
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