CN107011029A - Seedling agent and its production method are strengthened in a kind of green acid adjustment of high-effective microorganism - Google Patents
Seedling agent and its production method are strengthened in a kind of green acid adjustment of high-effective microorganism Download PDFInfo
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- CN107011029A CN107011029A CN201710219681.4A CN201710219681A CN107011029A CN 107011029 A CN107011029 A CN 107011029A CN 201710219681 A CN201710219681 A CN 201710219681A CN 107011029 A CN107011029 A CN 107011029A
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- strengthened
- seedling agent
- acid adjustment
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- seedling
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
Seedling agent and its production method are strengthened the invention discloses a kind of green acid adjustment of high-effective microorganism.The present invention cultivates aspergillus niger on PDA solid mediums, is linked into afterwards in seed culture medium, and fermentation obtains seed fermented liquid.Then seed fermentation liquid and the suitable fermentation medium to be seeded that has sterilized aseptically are inoculated with by a certain percentage and continue fermentation after be well mixed, produce the conidia powder of the microorganism.Finally again using turf as carrier, after being mixed with quantitative pyrolkigneous liquid and turfy soil, acidification is carried out.A certain amount of crushing of addition, the nitrogen mixed, phosphorus, potassium and trace element mix and produce the microbial-bacterial fertilizer.The present invention has activating soil, adjusts soil aciditiy, increase soil fertility and gas permeability, and killing pests and suppressing bacteria effect is good, nurturing staff, slow seedling is fast after transplanting, and tiller is early, is that the high yield of paddy rice creates the effect of good condition.It is that stalk recycles offer technical support and the soil-borne disease to multiple kinds of crops seedling stage plays certain preventive and therapeutic effect.
Description
Technical field:
Seedling bacterial manure is strengthened the present invention relates to a kind of micro organism green acid adjustment of agricultural technology field, is that one kind is suitable for a variety of farmings
Seedling agent is strengthened in the green acid adjustment of the solid microbe of thing, belongs to circular agriculture.
Background technology
For many years, in arviculture, the consumption of chemical fertilizer is being continuously increased, and the consumption of organic fertilizer is increasingly
It is few, and crops to the utilization rate of chemical fertilizer only 30% or so, about 70% chemical fertilizer deposition be fixed in soil or with
Rain water runoff is without being absorbed and being utilized by crops in rivers, and which results in soil hardening, environmental pollution, rivers and underground water
Negative nutrient laden is on the rise, and soil organic matter content reduction, fertility declines, and beneficial microorganism living space diminishes in soil,
Quantity is reduced, and the disease-resistant of crops, cold-resistant, drought-resistant ability weaken, and the generation of pest and disease damage is increasingly severe, causes crops quality
Decline, yield reduction.
In recent years, bio-organic fertilizer, composite microbiological fertilizer application it is increasingly extensive, particularly composite microbe fertilizer
Material is rapid to emerge, and this will make China's fertilizer industry enter a brand-new epoch, and composite microbiological fertilizer is applied in agricultural production
Show, in the raising of chemical fertilizer utilization rate, chesson reduces river pollution, culture fertility, nurturing staff, reduction disease pest
Extraordinary effect is suffered from terms of harmful generation, the resistance for strengthening crop.Pyrolkigneous liquid is interior as straw charring byproduct
Containing the gas chromatography matter such as acids, alcohols, phenols, environmental protection and public nuisance free changes soil aciditiy, and antibacterial desinsection, increase soil is organic
The effects such as matter, while depositing into the cycle applications of stalk.
The content of the invention:
It is an object of the invention to provide a kind of both environmentally friendly, there is increase soil fertility and gas permeability, strong stress resistance, improved soil again
Tiny ecosystem structure and the strong sprout that promotes, Qiang Gen, the strong seedling agent of microorganism for promoting the acid-regulating sterilization of resultant effect such as tiller, disease-resistant, volume increase.
The object of the present invention is achieved like this.Seedling agent, a kind of high-effective microorganism are strengthened in a kind of green acid adjustment of high-effective microorganism
Seedling agent is strengthened in green acid adjustment, it is characterised in that:Including following component:Aspergillus niger spore content is more than or equal to 2,000,000 cfu/g, nitre
Sour potassium(KNO3)15-30g/kg, ammonium nitrate(NH4NO3)12-20g/kg, magnesium sulfate(MgSO4·7H2O)2-5g/kg, calcium chloride
(CaCl2)2-5g/kg, potassium dihydrogen phosphate(KH2PO4)1-3g/kg, boric acid(H3BO3)0.045-0.085g/kg, zinc sulfate
(ZnSO4·7H2O)0.05-0.1g/kg, manganese sulfate(MnSO4·4H2O)0.1-0.3g/kg, which is mixed, produces the strong seedling of the microorganism
Agent.
Also include micro sodium molybdate in the strong seedling agent(NaMoO4·2H2O), copper sulphate(CuSO4·5H2O), chlorination
Cobalt, KI(KI).
Wherein preferred component is:Aspergillus niger spore content is more than or equal to 2,000,000 cfu/g, potassium nitrate(KNO3)19g/kg、
Ammonium nitrate(NH4NO3)16.5g/kg, magnesium sulfate(MgSO4·7H2O)3.7g/kg, calcium chloride(CaCl2)3.3g/kg, biphosphate
Potassium(KH2PO4)1.7g/kg, boric acid(H3BO3)0.062g/kg, zinc sulfate(ZnSO4·7H2O)0.086g/kg, manganese sulfate
(MnSO4·4H2O)0.223g/kg.
Wherein preferred component is:Sodium molybdate(NaMoO4·2H2O)0.0025g/kg, copper sulphate(CuSO4·5H2O)
0.00025g/kg, cobalt chloride(CoCl2·6H2O)0.00025g/kg, KI(KI)0.0083g/kg.
Described black-koji mould belongs to the fungi of production citric acid.
Its preparation method, it is characterised in that:Comprise the following steps:
Step one, take black-koji mould to be inoculated into the sterilized 500ml triangular flasks equipped with 200ml Czapek's mediums, shaking
In bed 2~3d is cultivated in 30 ± 1 DEG C, 200r/min;Produce seed fermentation liquid;
Step 2, by seed liquor and the suitable fermentation medium to be seeded sterilized by 1:1.5 ratio is aseptically
It is inoculated with and continues to ferment in incubator after being well mixed;
Step 3, after the completion of culture, takes out, and is placed in dry, acquisition aspergillus niger spore powder after sieving under the conditions of gravity-flow ventilation;
Step 4, using turf as carrier, after being mixed with quantitative pyrolkigneous liquid and turfy soil, carries out acidification, makes turfy soil pH
For 3.5 ± 1;Add a certain amount of aspergillus niger spore and nitrogen, phosphorus, potassium and trace element granulation produce the microorganism and strengthen seedling agent.
In the step 2, fermentation medium includes:Wheat bran 68.0-72.0%, corn flour 23.0-27.0%, phosphoric acid
Potassium dihydrogen 0.2%, ammonium sulfate 0.1%, calcium carbonate 0.1% and magnesium sulfate 0.1%;Wherein solid-liquid ratio is 1:1.5.
The fermentation medium is preferably in a proportion of:Wheat bran 70.0%, corn flour 25.0%, potassium dihydrogen phosphate 0.2%, sulfuric acid
Ammonium 0.1%, calcium carbonate 0.1% and magnesium sulfate 0.1%;Wherein solid-liquid ratio is 1:1.5.
The present invention provides a kind of green acid adjustment of high-effective microorganism and strengthens seedling agent, and biomass is aspergillus niger, described aspergillus niger
The cfu/g of viable count >=2,000,000.Described black-koji mould is a kind of generally recognized as safe (GRAS) microorganism, and pyrolkigneous liquid is straw charcoal
The accessory substance of change, it is nontoxic, without environmentally harmful material, acidity is adjusted, increases the soil organism, killing pests and suppressing bacteria, increase soil
Earth fertility, improves the resultant effects such as soil texture.Its preparation method is:Aspergillus niger is cultivated on PDA solid mediums, afterwards
It is linked into seed culture medium, fermentation obtains seed fermented liquid.Then by seed fermentation liquid and to be seeded fitting of sterilize
Suitable fermentation medium presses 1:1.5 ratio is aseptically inoculated with and continues to ferment after well mixed, produces the microorganism
Conidia powder.Finally again using turf as carrier, after being mixed with quantitative pyrolkigneous liquid and turfy soil, acidification is carried out, makes turfy soil
PH is 3.5 or so.A certain amount of crushing of addition, the nitrogen mixed, phosphorus, potassium and trace element mix and produce the microbial-bacterial fertilizer.Its
Preparation technology is simple, and cost is low, it is easy to preserves and easily realizes automated production etc..
Beneficial effects of the present invention:The present invention has activating soil, adjusts soil aciditiy, increases soil fertility and ventilative
Property, killing pests and suppressing bacteria effect is good, nurturing staff, slow seedling is fast after transplanting, and tiller is early, is that the high yield of paddy rice creates the work of good condition
With.It is that stalk recycles offer technology and the soil-borne disease to multiple kinds of crops seedling stage plays certain preventive and therapeutic effect
Support.
Brief description of the drawings
Fig. 1 is concrete operations flow chart of the invention.
Embodiment
With reference to specific embodiment, the present invention is further illustrated
Embodiment 1
Seedling agent is strengthened in a kind of green acid adjustment of high-effective microorganism, and seedling agent is strengthened in a kind of green acid adjustment of high-effective microorganism, it is characterised in that:Including
Following component:Aspergillus niger spore content is more than or equal to 2,000,000 cfu/g, potassium nitrate(KNO3)15-30g/kg, ammonium nitrate
(NH4NO3)12-20g/kg, magnesium sulfate(MgSO4·7H2O)2-5g/kg, calcium chloride(CaCl2)2-5g/kg, potassium dihydrogen phosphate
(KH2PO4)1-3g/kg, boric acid(H3BO3)0.045-0.085g/kg, zinc sulfate(ZnSO4·7H2O)0.05-0.1g/kg, sulfuric acid
Manganese(MnSO4·4H2O)0.1-0.3g/kg, which is mixed, produces the strong seedling agent of the microorganism.
Also include micro sodium molybdate in the strong seedling agent(NaMoO4·2H2O), copper sulphate(CuSO4·5H2O), chlorination
Cobalt, KI(KI).
Wherein preferred component is:Aspergillus niger spore content is more than or equal to 2,000,000 cfu/g, potassium nitrate(KNO3)19g/kg、
Ammonium nitrate(NH4NO3)16.5g/kg, magnesium sulfate(MgSO4·7H2O)3.7g/kg, calcium chloride(CaCl2)3.3g/kg, biphosphate
Potassium(KH2PO4)1.7g/kg, boric acid(H3BO3)0.062g/kg, zinc sulfate(ZnSO4·7H2O)0.086g/kg, manganese sulfate
(MnSO4·4H2O)0.223g/kg.
Wherein preferred component is:Sodium molybdate(NaMoO4·2H2O)0.0025g/kg, copper sulphate(CuSO4·5H2O)
0.00025g/kg, cobalt chloride(CoCl2·6H2O)0.00025g/kg, KI(KI)0.0083g/kg.
Described black-koji mould belongs to the fungi of production citric acid.
Embodiment 2
Its preparation method, it is characterised in that:Comprise the following steps:
Step one, take black-koji mould to be inoculated into the sterilized 500ml triangular flasks equipped with 200ml Czapek's mediums, shaking
In bed 2~3d is cultivated in 30 ± 1 DEG C, 200r/min;Produce seed fermentation liquid;
Step 2, by seed liquor and the suitable fermentation medium to be seeded sterilized by 1:1.5 ratio is aseptically
It is inoculated with and continues to ferment in incubator after being well mixed;
Step 3, after the completion of culture, takes out, and is placed in dry, acquisition aspergillus niger spore powder after sieving under the conditions of gravity-flow ventilation;
Step 4, using turf as carrier, after being mixed with quantitative pyrolkigneous liquid and turfy soil, carries out acidification, makes turfy soil pH
For 3.5 ± 1;Add a certain amount of aspergillus niger spore and nitrogen, phosphorus, potassium and trace element granulation produce the microorganism and strengthen seedling agent.
Embodiment 3
In the step 2, fermentation medium includes:Wheat bran 68.0-72.0%, corn flour 23.0-27.0%, biphosphate
Potassium 0.2%, ammonium sulfate 0.1%, calcium carbonate 0.1% and magnesium sulfate 0.1%;Wherein solid-liquid ratio is 1:1.5.
The fermentation medium is preferably in a proportion of:Wheat bran 70.0%, corn flour 25.0%, potassium dihydrogen phosphate 0.2%, sulfuric acid
Ammonium 0.1%, calcium carbonate 0.1% and magnesium sulfate 0.1%;Wherein solid-liquid ratio is 1:1.5.
Embodiment 4
The step one, trains the black-koji mould on inclined-plane by purified aspergillus niger PDA solids, is inoculated into sterilized
In 500ml triangular flasks equipped with 200ml Czapek's mediums, in 30 ± 1 in shaking table, DEG C, 200r/min cultivate 2~3d;Produce
Seed fermentation liquid;
Step 2, by seed liquor and the suitable fermentation medium to be seeded sterilized(Wheat bran 70.0%, corn flour 25.0%,
Potassium dihydrogen phosphate 0.2%, ammonium sulfate 0.1%, calcium carbonate 0.1% and magnesium sulfate 0.1%)By 1:1.5 ratio is in aseptic condition
It is lower to be inoculated with and continue to ferment in incubator after well mixed;
Step 3, after the completion of culture, takes out, and is placed in drying under the conditions of gravity-flow ventilation;Dry 5-6 days, humidity is less than or equal to 10%,
Aspergillus niger spore powder can be obtained after sieving;
Step 4, first weighs turfy soil after fully drying by component proportion, and cement pit is built up in operation, with calmly
After the pyrolkigneous liquid of amount is mixed with turfy soil, acidification is carried out, it is 3.5 or so to make turfy soil pH.By operational procedure safety operation,
Then heap is vexed 21-23 hours, then scatters and dry in the air 2-3 hours, a large amount of members for add aspergillus niger spore powder and crushing by proportioning, mixing
Element:Potassium nitrate(KNO3)19g/kg, ammonium nitrate(NH4NO3)16.5g/kg, magnesium sulfate(MgSO4·7H2O)3.7g/kg, calcium chloride
(CaCl2)3.3g/kg, potassium dihydrogen phosphate(KH2PO4)1.7g/kg;Trace element:Boric acid(H3BO3)0.062g/kg, zinc sulfate
(ZnSO4·7H2O)0.086g/kg, manganese sulfate(MnSO4·4H2O)0.223g/kg, sodium molybdate(NaMoO4·2H2O)
0.0025g/kg, copper sulphate(CuSO4·5H2O)0.00025g/kg, cobalt chloride(CoCl2·6H2O)0.00025g/kg, iodate
Potassium(KI)0.0083g/kg);The strong seedling agent of the microorganism is produced by being sufficiently stirred for, sieving, check the weight, pack, the microorganism strengthens seedling
500g/ bags of agent.
Embodiment 5
Concrete operations flow chart is as shown in Figure 1.
Essence for a better understanding of the present invention, is provided below application process and effect that the solid microbe strengthens seedling agent
Really.
This product is mainly mixed soil in crop seedling, seedling separation, field planting and used.
1. nursery:500 grams of this product is mixed uniformly with 15-30 kilograms of fine earths, is sprinkled into and shallow on 10-15 square metres of bed surface is turned over 5
Centimetre, an animal-drawn seed plough is flat water after can sow, be covered with 1 centimeter of pure land again above.
2. nutritive cube seedling separation:500 grams of this product is mixed uniformly with 1 cubic metre of Nutrition Soil, fills nutritive cube(By taking 8 × 8cm as an example),
1000~1500 young plants.
3. it is colonized, spreads manuer in holes:After 500 grams of this product is mixed uniformly with 50 kilograms of fine earths, the root or cave of crop are equably applied
It is interior, you can field planting is sowed, and every bag can apply 1000 young plants or so.
Claims (8)
1. seedling agent is strengthened in a kind of green acid adjustment of high-effective microorganism, seedling agent is strengthened in a kind of green acid adjustment of high-effective microorganism, it is characterised in that:Bag
Include following component:Aspergillus niger spore content is more than or equal to 2,000,000 cfu/g, potassium nitrate(KNO3)15-30g/kg, ammonium nitrate
(NH4NO3)12-20g/kg, magnesium sulfate(MgSO4·7H2O)2-5g/kg, calcium chloride(CaCl2)2-5g/kg, potassium dihydrogen phosphate
(KH2PO4)1-3g/kg, boric acid(H3BO3)0.045-0.085g/kg, zinc sulfate(ZnSO4·7H2O)0.05-0.1g/kg, sulfuric acid
Manganese(MnSO4·4H2O)0.1-0.3g/kg, which is mixed, produces the strong seedling agent of the microorganism.
2. seedling agent is strengthened in the green acid adjustment of a kind of high-effective microorganism according to claim 1, it is characterised in that:The strong seedling agent
In also include micro sodium molybdate(NaMoO4·2H2O), copper sulphate(CuSO4·5H2O), cobalt chloride, KI(KI).
3. seedling agent is strengthened in the green acid adjustment of a kind of high-effective microorganism according to claim 1, it is characterised in that:Wherein preferably into
It is divided into:Aspergillus niger spore content is more than or equal to 2,000,000 cfu/g, potassium nitrate(KNO3)19g/kg, ammonium nitrate(NH4NO3)
16.5g/kg, magnesium sulfate(MgSO4·7H2O)3.7g/kg, calcium chloride(CaCl2)3.3g/kg, potassium dihydrogen phosphate(KH2PO4)
1.7g/kg, boric acid(H3BO3)0.062g/kg, zinc sulfate(ZnSO4·7H2O)0.086g/kg, manganese sulfate(MnSO4·4H2O)
0.223g/kg。
4. seedling agent is strengthened in the green acid adjustment of a kind of high-effective microorganism according to claim 2, it is characterised in that:Wherein preferably into
It is divided into:Sodium molybdate(NaMoO4·2H2O)0.0025g/kg, copper sulphate(CuSO4·5H2O)0.00025g/kg, cobalt chloride
(CoCl2·6H2O)0.00025g/kg, KI(KI)0.0083g/kg.
5. seedling agent is strengthened in the green acid adjustment of a kind of high-effective microorganism according to claim 1, it is characterised in that:Described black song
Mould belongs to the fungi of production citric acid.
6. seedling agent preparation method is strengthened in the green acid adjustment of a kind of high-effective microorganism according to claim 1-5, it is characterised in that:
Comprise the following steps:
Step one, take black-koji mould to be inoculated into the sterilized 500ml triangular flasks equipped with 200ml Czapek's mediums, shaking
In bed 2~3d is cultivated in 30 ± 1 DEG C, 200r/min;Produce seed fermentation liquid;
Step 2, by seed liquor and the suitable fermentation medium to be seeded sterilized by 1:1.5 ratio is aseptically
It is inoculated with and continues to ferment in incubator after being well mixed;
Step 3, after the completion of culture, takes out, and is placed in dry, acquisition aspergillus niger spore powder after sieving under the conditions of gravity-flow ventilation;
Step 4, using turf as carrier, after being mixed with quantitative pyrolkigneous liquid and turfy soil, carries out acidification, makes turfy soil pH
For 3.5 ± 1;Add a certain amount of aspergillus niger spore and nitrogen, phosphorus, potassium and trace element granulation produce the microorganism and strengthen seedling agent.
7. seedling agent preparation method is strengthened in the green acid adjustment of a kind of high-effective microorganism according to claim 6, it is characterised in that:It is described
In step 2, fermentation medium includes:Wheat bran 68.0-72.0%, corn flour 23.0-27.0%, potassium dihydrogen phosphate 0.2%,
Ammonium sulfate 0.1%, calcium carbonate 0.1% and magnesium sulfate 0.1%;Wherein solid-liquid ratio is 1:1.5.
8. seedling agent preparation method is strengthened in the green acid adjustment of a kind of high-effective microorganism according to claim 7, it is characterised in that:It is described
Fermentation medium is preferably in a proportion of:Wheat bran 70.0%, corn flour 25.0%, potassium dihydrogen phosphate 0.2%, ammonium sulfate 0.1%, carbonic acid
Calcium 0.1% and magnesium sulfate 0.1%;Wherein solid-liquid ratio is 1:1.5.
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Citations (4)
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---|---|---|---|---|
CN1294106A (en) * | 1999-10-29 | 2001-05-09 | 刘敏 | Nutritive liquid for plant |
CN101723746A (en) * | 2008-10-24 | 2010-06-09 | 黑龙江省科学院生物肥料研究中心 | Novel seedling imigorator for rice |
CN103555593A (en) * | 2013-11-07 | 2014-02-05 | 沂源康源生物科技有限公司 | Preparation method of Aspergillus niger strain and spore powder |
CN103773719A (en) * | 2013-12-30 | 2014-05-07 | 邵素英 | Preparation method of microbial compound fertilizer |
-
2017
- 2017-04-06 CN CN201710219681.4A patent/CN107011029A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1294106A (en) * | 1999-10-29 | 2001-05-09 | 刘敏 | Nutritive liquid for plant |
CN101723746A (en) * | 2008-10-24 | 2010-06-09 | 黑龙江省科学院生物肥料研究中心 | Novel seedling imigorator for rice |
CN103555593A (en) * | 2013-11-07 | 2014-02-05 | 沂源康源生物科技有限公司 | Preparation method of Aspergillus niger strain and spore powder |
CN103773719A (en) * | 2013-12-30 | 2014-05-07 | 邵素英 | Preparation method of microbial compound fertilizer |
Non-Patent Citations (1)
Title |
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杨晓兰 等: "黑曲霉固体发酵条件的优化", 《养殖与饲料》 * |
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