CN107006686A - A kind of preparation method and application of the food dusty yeast with adhesive effect - Google Patents

A kind of preparation method and application of the food dusty yeast with adhesive effect Download PDF

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Publication number
CN107006686A
CN107006686A CN201710286795.0A CN201710286795A CN107006686A CN 107006686 A CN107006686 A CN 107006686A CN 201710286795 A CN201710286795 A CN 201710286795A CN 107006686 A CN107006686 A CN 107006686A
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food
feed
preparation
dusty yeast
yeast
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Inventor
朱正鹏
郭吉原
李芳溢
燕磊
赵敏
吕尊周
安沙
王正国
吴月明
朱玉涛
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New Hope Liuhe Co Ltd
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New Hope Liuhe Co Ltd
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Abstract

The invention discloses a kind of preparation method and application of the food dusty yeast with adhesive effect, wherein preparation method is:Beet root is cleaned after chopping, addition water and salt are soaked, and are filtrated to get diffusion juice;Diffusion juice is stood and is cooled to room temperature, calcium superphosphate, saccharomycete seed liquor are then added into diffusion juice, mixed liquor is obtained, mixed liquor is kept into 12 20h, zymotic fluid is obtained;Dry:Obtained separation of fermentative broth is obtained into yeast wastewater, yeast wastewater is passed through in spray dryer and is dried, food dusty yeast is obtained.The beneficial effects of the invention are as follows:Food dusty yeast is obtained by preparation method of the present invention, with extraordinary adhesive effect, the fields such as food and feed are can be applied to;Applied to field of feed processing, not only ensure the nutritional need of animal, but also can be good at improving the characteristic of feed physical behavior, effectively improve efficiency of feed utilization, alleviate the feedstuff supply pressures such as flour, promote aquaculture to develop in a healthy way.

Description

A kind of preparation method and application of the food dusty yeast with adhesive effect
Technical field
The present invention relates to field of feed, more particularly to a kind of utilization yeast wastewater prepares the method for adhesive and answered With.
Background technology
Intensive, industrial aquaculture industry development make it that the research to animal feed additive is also increasingly deep, among these Both included that the nutritive additive of Animal nutrition can be provided, also including raising to enhancing development, improvement feed structure, holding Material quality, promotion are digested and assimilated, the non-nutritive additive of disease resistance, such as growth accelerator, dusty yeast, phagostimulant.
Wherein feed is to maintain mixed feed graininess with the main function of adhesive, is easy to animal ingestion, especially uses Lost in preventing that feed nutrient is molten in water, be easy to fish, improve feed efficiency, prevent water quality deterioration.At present, feed The starch such as flour, wheat, corn, cassava, the potato of middle addition or converted starch are respectively provided with certain bonding effect, but it adds Dosage is big, and shared formulation space is big, and the size of its cohesive force, depending on starch type and feed-processing equipment and operation Technology.
The content of the invention
The goal of the invention of the present invention is to provide that a kind of bonding effect is good, consumption is few, cost is low and applicable all feeds adds The preparation method and application of the food dusty yeast of work method.
In order to realize foregoing invention purpose, the invention provides a kind of system of the food dusty yeast with adhesive effect Preparation Method, the preparation method is:
(1)Extraction:Beet root is cleaned after chopping, addition water and salt are soaked, the addition of water is beet root gross weight 2-3 times, the addition of salt is beet root and the 1-3% of water gross weight;Soaking temperature is 55-70 DEG C, and pH value is 5.0-6.5, Diffusion juice is filtrated to get after immersion 4-5h;In immersion process, addition bactericidal agent and defoamer;
(2)Fermentation:By step(1)Obtained diffusion juice stands and is cooled to room temperature, then into diffusion juice addition to account for diffusion juice total Weight 0.2-2% calcium superphosphate, 20-30% saccharomycete seed liquor, obtain mixed liquor, by mixed liquor control in throughput 2L/ Under conditions of min, zymotic fluid pH5.5-7.0,28-33 DEG C of fermentation temperature, 12-20h is maintained, zymotic fluid is obtained;Wherein, in fermentation During, stir per hour once, 5min is stirred every time;
(3)Dry:By step(2)Obtained separation of fermentative broth obtains yeast wastewater, and yeast wastewater is passed through in spray dryer Row drying, obtains food dusty yeast;Wherein, the inlet air temperature of spray dryer is 220-270 DEG C, and outlet air temperature is 105-110℃。
Wherein, the step(1)In extraction, the bactericidal agent is the ethanol that volumetric concentration is 75%, and the bactericidal agent adds Dosage is beet root and the 0.5-2% of water gross weight, preferably 1%;The defoamer is polypropylene-polyethylene glycerin ether, addition For 0.2-0.32kg/t, preferably 0.28kg/t.
Present invention also offers a kind of application of feed adhesive of above-mentioned preparation method in feed manufacturing.
In order to preferably realize foregoing invention purpose, contain above-mentioned preparation method food dusty yeast the invention provides one kind Feed producing method, the feed producing method is:
(1)Batching step:Each raw material of feed is weighed, then food yeast is weighed according to feedstuff gross weight 0.5-3% proportioning Powder, mixer is put into each raw material in the lump, is well mixed, incorporation time is 30-60s;
(2)Granulation step:By step(1)Pelletized in well mixed material input granulator, pelleting temperature is 75-80 DEG C, its gelatinization degree is reached more than 90%.
The invention provides a kind of preparation method of the broiler fodder containing the food dusty yeast, specifically, the broiler chicken is raised The preparation method of material is:
(1)Batching step:Each raw material of feed is weighed, then food yeast is weighed according to feedstuff gross weight 0.5-3% proportioning Powder, mixer is put into each raw material in the lump, is well mixed, incorporation time is 30-60s;
(2)Granulation step:By step(1)Pelletized in well mixed material input granulator, pelleting temperature is 75-80 DEG C, its gelatinization degree is reached more than 90%;
Wherein, the raw material is specially:Corn 59%, dregs of beans 28.5%, corn DDGS 2.5%, Cottonseed Meal 1.74%, corn protein powder 2.7%th, calcium monohydrogen phosphate 1.8%, stone flour 1.0%, 1B sulfate 0.7%, soya-bean oil 1%, sodium chloride 0.25%, methionine 0.18%, Threonine 0.23%, Choline Chloride 0.2%, sodium acid carbonate 0.2%.
Present invention also offers a kind of preparation method of the carp feed containing the food dusty yeast, specifically, the carp The preparation method of feed is:
(1)Batching step:Each raw material of feed is weighed, then food yeast is weighed according to feedstuff gross weight 0.5-3% proportioning Powder, mixer is put into each raw material in the lump, is well mixed, incorporation time is 30-60s;
(2)Granulation step:By step(1)Pelletized in well mixed material input granulator, pelleting temperature is 75-80 DEG C, its gelatinization degree is reached more than 90%.
Wherein, the raw material is specially:Dregs of beans 26%, flour 7%, wheat bran 6%, rapeseed cake 19%, maize germ cake 11%, DDGS 16%th, fish meal 5%, allicin 1.5%, haslet powder 3%, Shrimp chaff powder 1%, soya-bean oil 3%, calcium dihydrogen phosphate 1%, stone flour 0.29%, DL- eggs Propylhomoserin 0.04%, Choline Chloride 0.15%, Pulvis Fellis Suis 0.02%.
The beneficial effects of the invention are as follows:Food dusty yeast is obtained by preparation method of the present invention, with extraordinary adhesive effect, It can be applied to the fields such as food and feed;When food dusty yeast is applied into feed manufacturing, the normal of feed can not only be ensured Nutritive value, meets the normal nutrition demand of animal, but also can be good at improving the characteristic of feed physical behavior, effectively carries High efficiency of feed utilization, alleviates the supply pressure of the feedstuffs such as flour, promotes aquaculture to develop in a healthy way;Wherein, the present invention's In preparation method, glycine betaine is rich in beet root, and glycine betaine is a kind of effective phagostimulant, can promote animal ingestion, is carried Seedling height performance;And step(3)Isolated yeast wastewater is by drying the dusty yeast obtained in drying steps, it is possible to increase Animal body immunity, keeps gut integrity, but also with good natural attractant, applyFeedIn can improve Feed physical behavior, and strengthen the palatability of animal feed;And the yeast milk obtained after separating can continue to be processed into traditional food Product dusty yeast, produces food dusty yeast of the present invention using yeast wastewater, reduces production cost.
Embodiment
For the technical characterstic for illustrating this programme can be understood, below by embodiment, this programme is illustrated.
The preparation method of the food dusty yeast of embodiment 1
The embodiments of the invention provide a kind of preparation method of the food dusty yeast with adhesive effect, it is specially:
(1)Extraction:Beet root is cleaned after chopping, addition water and salt are soaked, the addition of water is beet root gross weight 2.5 times, the addition of salt is the 2% of beet root and water gross weight;Soaking temperature is 65 DEG C, and pH value is 5.0-6.5, immersion Diffusion juice is filtrated to get after 4.5h;In immersion process, addition bactericidal agent and defoamer;Wherein, bactericidal agent is that volumetric concentration is 75% ethanol, addition is the 1% of beet root and water gross weight;Defoamer is polypropylene-polyethylene glycerin ether, and addition is 0.28kg/t。
(2)Fermentation:By step(1)Obtained diffusion juice stands and is cooled to room temperature, then adds to account for into diffusion juice and oozes out The calcium superphosphate of juice gross weight 1%, 25% saccharomycete seed liquor, obtain mixed liquor, by mixed liquor control throughput 2L/min, Under conditions of zymotic fluid pH5.5-7.0,30 DEG C of fermentation temperature, 16h is maintained, zymotic fluid is obtained;Wherein, during the fermentation, often Hour stirring once, stirs 5min every time;
(3)Dry:By step(2)Obtained separation of fermentative broth obtains yeast wastewater, and yeast wastewater is passed through in spray dryer Row drying, obtains food dusty yeast;Wherein, the inlet air temperature of spray dryer is 250 DEG C, and outlet air temperature is 108 ℃。
The preparation method of the food dusty yeast of embodiment 2
The embodiments of the invention provide a kind of preparation method of the food dusty yeast with adhesive effect, it is specially:
(1)Extraction:Beet root is cleaned after chopping, addition water and salt are soaked, the addition of water is beet root gross weight 3 times, the addition of salt is the 3% of beet root and water gross weight;Soaking temperature is 60 DEG C, and pH value is 5.0-6.5, soaks 5h After be filtrated to get diffusion juice;In immersion process, addition bactericidal agent and defoamer;Wherein, bactericidal agent is that volumetric concentration is 75% Ethanol, the addition of the bactericidal agent is the 1.8% of beet root and water gross weight;The defoamer is polypropylene-polyethylene glycerine Ether, addition is 0.2kg/t.
(2)Fermentation:By step(1)Obtained diffusion juice stands and is cooled to room temperature, then adds to account for into diffusion juice and oozes out The calcium superphosphate of juice gross weight 1.5%, 25% saccharomycete seed liquor, obtain mixed liquor, by mixed liquor control in throughput 2L/ Under conditions of min, zymotic fluid pH5.5-7.0,33 DEG C of fermentation temperature, 20h is maintained, zymotic fluid is obtained;Wherein, in fermentation process In, stir per hour once, 5min is stirred every time;
(3)Dry:By step(2)Obtained separation of fermentative broth obtains yeast wastewater, and yeast wastewater is passed through in spray dryer Row drying, obtains food dusty yeast;Wherein, the inlet air temperature of spray dryer is 250 DEG C, and outlet air temperature is 110 ℃。
The broiler fodder of embodiment 3
The embodiments of the invention provide a kind of preparation method of the broiler fodder containing food dusty yeast, it is specially:
(1)Batching step:Each raw material of feed is weighed, then food dusty yeast is weighed according to the proportioning of feedstuff gross weight 1%, Mixer is put into the lump with each raw material, is well mixed, incorporation time is 40s;
(2)Granulation step:By step(1)Pelletized in well mixed material input granulator, pelleting temperature is 75-80 DEG C, its gelatinization degree is reached more than 90%.
Wherein, above-mentioned food dusty yeast is obtained by the preparation method of embodiment 1.
Above-mentioned broiler fodder is made up of the raw material of following percentage by weight:Corn 59%, dregs of beans 28.5%, corn DDGS2.5%, Cottonseed Meal 1.74%, corn protein powder 2.7%, calcium monohydrogen phosphate 1.8%, stone flour 1.0%, 1B sulfate 0.7%, Soya-bean oil 1%, sodium chloride 0.25%, methionine 0.18%, threonine 0.23%, Choline Chloride 0.2%, sodium acid carbonate 0.2%.
The carp feed of embodiment 4
The embodiments of the invention provide a kind of preparation method of the carp feed containing food dusty yeast, it is specially:
(1)Batching step:Each raw material of feed is weighed, then food yeast is weighed according to the proportioning of feedstuff gross weight 1.5% Powder, mixer is put into each raw material in the lump, is well mixed, incorporation time is 60s;
(2)Granulation step:By step(1)Pelletized in well mixed material input granulator, pelleting temperature is 75-80 DEG C, its gelatinization degree is reached more than 90%.
Wherein, above-mentioned food dusty yeast is obtained by the preparation method of embodiment 2;
Above-mentioned carp feed is made up of the raw material of following percentage by weight:Dregs of beans 26%, flour 7%, wheat bran 6%, rapeseed cake 19%, corn Germ cake 11%, DDGS 16%, fish meal 5%, allicin 1.5%, haslet powder 3%, Shrimp chaff powder 1%, soya-bean oil 3%, calcium dihydrogen phosphate 1%, Stone flour 0.29%, DL- METHIONINE 0.04%, Choline Chloride 0.15%, Pulvis Fellis Suis 0.02%.
Contrast test
First, the contrast test such as feed nutrient and physical behavior
By the broiler fodder of embodiment 3(Embodiment group A), embodiment 4 carp feed(Embodiment group B)And it is not added with this hair The broiler fodder of bright food dusty yeast(Control group A)And carp feed(Control group B)Nutrient component determining is carried out, measurement result is such as Shown in table 1, and physical behavior measure is carried out to the feed of four experimental groups respectively, as a result as shown in table 2.
Each experimental group feed nutrient table of comparisons of table 1
Project Embodiment group A Control group A Embodiment group B Control group B
Crude protein % 22.02 21.99 34.12 34.28
Fatty % 3.05 3.01 10.05 10.09
Moisture content % 12.98 13.00 8.47 8.27
Ash content % 5.65 5.50 7.03 7.19
Calcium % 0.94 0.93 0.91 0.92
Phosphorus % 0.64 0.64 1.35 1.34
Calcium-phosphorus ratio 1.47 1.45 0.67 0.69
By above-mentioned data, it can be seen that the feed of the embodiment of the present invention 3 and embodiment 4 containing food dusty yeast, nutritional ingredient is such as Crude protein, fat and calcium etc. and traditional broiler fodder and carp feed are basically identical, therefore, the embodiment of the present invention 3 and embodiment 4 Feed can be good at meeting the nutritional needs of the animals such as broiler chicken, carp.
Each experimental group feed physical behavior table of comparisons of table 2
Project Embodiment group A Control group A Embodiment group B Control group B
Feed hardness kg 5.05 3.22 4.05 2.62
Pulverization rate % 1.2 2.6 0.44 1.60
The % of rate containing powder - - 0.32 1.52
Water resistance % - - 27.85 19.65
By above-mentioned data, it can be seen that compared with traditional broiler fodder, broiler chicken of the embodiment of the present invention 3 containing food dusty yeast is raised Material hardness is improved, and pulverization rate is significantly reduced;Carp feed of the embodiment of the present invention 4 containing food dusty yeast is hard in feed Traditional carp feed is significantly better than that in terms of degree, pulverization rate, rate containing powder and water resistance.
2nd, the table of comparisons that feed influences on breeding performonce fo animals
(1)Experimental animal is only used as from the commercial broiler 2400 of 1 age in days Ross 308,2 test groups are randomly divided into, each group 4 Repeat, it is each to repeat 300.The broiler fodder of embodiment 3 is respectively adopted in 2 test groups(Embodiment group A)Raised with traditional broiler chicken Material(Control group A)Fed, fed to 38 age in days off-tests.
Feeding management program is pressed during experiment《Feeding of broiler administrative manual》Carry out, hand fed(Limit is raised, free water), And excrement and tank are cleared up daily.Observation chicken group situation, records survival rate daily.Whole column experimental chicken is carried out in the age in days of broiler chicken 38 Weigh on an empty stomach and count feed intake, calculate average daily gain, average daily gain, feed-weight ratio, experimental data is as shown in table 3.
Wherein, traditional broiler fodder that control group is used for embodiment 3 in be not added with the feed of food dusty yeast.
Each test group growth of meat chicken index table of comparisons of table 3
Project Embodiment group A Control group A
38 ages in days end weight(g) 2302 2189
Feed-weight ratio 1.515 1.536
From the data of table 3:The chick fed using the broiler fodder containing food dusty yeast of the embodiment of the present invention 3, extremely 38 ages in days end weight is apparently higher than the chick fed using traditional broiler fodder, and feed-weight ratio is substantially less than using traditional broiler fodder The chick of nursing.
(2)From the tail of healthy carp 360 that size, weight are basically identical, 2 groups, every group of 180 tails, every group of setting 6 are randomly divided into Repeat.The carp feed of the embodiment of the present invention 4 is respectively adopted in 2 test groups(Embodiment group B)With traditional carp feed(Control group B), feed experiment in 56 days and terminate.
Routinely manage temporarily to support before experiment and tamed for 2 weeks, during domestication, each test group feeds conventional feed;Daily Artificial be satiated with food feeds four times(8:00、11:00、14:00、17:00), 10 feeds of input, treat that fry robs food and finished every time, continue Input 10, is circulated successively, and observation fry ingests situation, when part fish dissociates Feeding Area, feeding volume is suitably reduced, until 2/3 Test fish is left, you can stopping is fed, and feeding volume is adjusted at any time according to water temperature and water quality situation during raising.Carry out daily carp Rate of body weight gain, specific growth rate, feed coefficient, the influence of survival rate and coefficient of condition, result of the test are as shown in table 4.
Wherein, traditional carp feed that control group is used is made up of the raw material of following percentage by weight:Dregs of beans 26%, flour 9%th, wheat bran 8%, rapeseed cake 19%, maize germ cake 12%, DDGS 16%, fish meal 5.5%, soya-bean oil 3%, calcium dihydrogen phosphate 1%, stone flour 0.29%th, DL- METHIONINE 0.04%, Choline Chloride 0.15%, Pulvis Fellis Suis 0.02%.
Each test group carp growth index table of comparisons of table 4
Project Embodiment group B Control group B P values
Initial counterpoise(g) 16.00±0.04 15.98±0.03 0.082
Whole end counterpoise(g) 83.92±2.61a 77.94±1.86b 0.007
Survival rate(%) 100.00±0.00 99.17±2.04 0.391
Specific growth rate(%/d) 3.02±0.16a 2.83±0.04b 0.009
Feed coefficient 1.14±0.06b 1.30±0.04a 0.008
Protein efficiency ratio, PER % 2.77±0.19a 2.43±0.08b 0.006
From the data of table 4:Compared with traditional carp material, raised using carp of the embodiment of the present invention 4 containing food dusty yeast Expect the carp fed, survival rate is higher by control group apparently higher than control group, eventually end counterpoise, specific growth rate and protein efficiency ratio, PER 5.98g, 0.19 %/d and 0.34 %, feed coefficient are substantially less than control group 0.16.
In summary, the food dusty yeast that Example 1 and Example 2 of the present invention is provided has extraordinary adhesive effect, by it Applied in terms of feed manufacturing, the original nutritive value of feed can not only be ensured, the normal nutrition demand of animal is met, and Feed physical behavior can also be obviously improved, the utilization rate of feed is improved.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in the spirit and principles in the present invention Within, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.

Claims (4)

1. a kind of preparation method of the food dusty yeast with adhesive effect, it is characterised in that the preparation method is:
(1)Extraction:Beet root is cleaned after chopping, addition water and salt are soaked, the addition of water is beet root gross weight 2-3 times, the addition of salt is beet root and the 1-3% of water gross weight;Soaking temperature is 55-70 DEG C, and pH value is 5.0-6.5, Diffusion juice is filtrated to get after immersion 4-5h;In immersion process, addition bactericidal agent and defoamer;
(2)Fermentation:By step(1)Obtained diffusion juice stands and is cooled to room temperature, then into diffusion juice addition to account for diffusion juice total Weight 0.2-2% calcium superphosphate, 20-30% saccharomycete seed liquor, obtain mixed liquor, by mixed liquor control in throughput 2L/ Under conditions of min, zymotic fluid pH5.5-7.0,28-33 DEG C of fermentation temperature, 12-20h is maintained, zymotic fluid is obtained;Wherein, in fermentation During, stir per hour once, 5min is stirred every time;
(3)Dry:By step(2)Obtained separation of fermentative broth obtains yeast wastewater, and yeast wastewater is passed through in spray dryer Row drying, obtains food dusty yeast;Wherein, the inlet air temperature of spray dryer is 220-270 DEG C, and outlet air temperature is 105-110℃。
2. the preparation method of the food dusty yeast according to claim 1 with adhesive effect, it is characterised in that the step Suddenly(1)In extraction, the bactericidal agent is the ethanol that volumetric concentration is 75%, and the addition of the bactericidal agent is that beet root and water are total The 0.5-2% of weight;The defoamer is polypropylene-polyethylene glycerin ether, and addition is 0.2-0.32kg/t.
3. a kind of food dusty yeast of preparation method as claimed in claim 1 or 2 is used as the application of adhesive in feed manufacturing.
4. a kind of feed producing method containing the food dusty yeast of preparation method as described in claim any one of 1-3, it is special Levy and be, the feed producing method is:
(1)Batching step:Each raw material of feed is weighed, then the food is weighed according to feedstuff gross weight 0.5-1.5% proportioning Product dusty yeast, mixer is put into each raw material in the lump, is well mixed, incorporation time is 30-60s;
(2)Granulation step:By step(1)Pelletized in well mixed material input granulator, pelleting temperature is 75-80 DEG C, its gelatinization degree is reached more than 90%.
CN201710286795.0A 2017-04-27 2017-04-27 A kind of preparation method and application of the food dusty yeast with adhesive effect Pending CN107006686A (en)

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