CN106995784A - The culture drop producing device of Embryo Culture ware - Google Patents
The culture drop producing device of Embryo Culture ware Download PDFInfo
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- CN106995784A CN106995784A CN201710153565.7A CN201710153565A CN106995784A CN 106995784 A CN106995784 A CN 106995784A CN 201710153565 A CN201710153565 A CN 201710153565A CN 106995784 A CN106995784 A CN 106995784A
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- tray motor
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 44
- 239000012531 culture fluid Substances 0.000 claims abstract description 28
- 235000015097 nutrients Nutrition 0.000 claims abstract description 22
- 239000012530 fluid Substances 0.000 claims abstract description 4
- 230000001681 protective effect Effects 0.000 claims description 7
- 230000013020 embryo development Effects 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 19
- 238000012549 training Methods 0.000 description 7
- 210000002683 foot Anatomy 0.000 description 4
- 230000004224 protection Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 210000003464 cuspid Anatomy 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000003648 hair appearance Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Producing device is dripped the present invention relates to a kind of culture of Embryo Culture ware, including workbench, push-rod electric machine, internal thread cylinder, external screw thread push rod, cultivate fluid cylinder, rotating disk, rotary tray motor and and piston head, workbench includes work top, installing plate and support feet, push-rod electric machine is installed on a mounting board by connector I, culture fluid cylinder bottom is additionally provided with drop outlets, cultivate fluid cylinder by connector II on a mounting board, the output shaft of push-rod electric machine is connected with internal thread cylinder, external screw thread push rod top is arranged in internal thread cylinder, external screw thread push rod lower end is connected with piston head and is located in culture fluid cylinder, rotary tray motor is arranged on work top, rotating disk is driven by the output shaft of rotary tray motor and is located at below drop outlets.The present invention ensure that the amount of every drop nutrient solution droplet size and covering culture oil is essentially identical, it is to avoid the error that human factor is caused during Embryo Culture ware culture dripping work, optimizes Embryo Culture environment, improves embryonic development quality.
Description
Technical field
Producing device is dripped the present invention relates to a kind of culture of Embryo Culture ware.
Background technology
Culture dish used in current Embryo Culture is plastic culture dish.Lab assistant is trained on culture dish bottom marker
Nutrient solution droplet number, the nutrient solution droplet that volume is 20 microlitres, Ran Hou are made in corresponding droplet number field with nutrient solution
Covering culture oil above Medium drop.Lab assistant relays embryonated egg from the droplet that culture dish is moved on to by seminal fluid transfer
It is continuous to grow, until the 3rd day embryo is freezed or embryo transfer.Embryo is transplanted in the 3rd day patient selection,
Freezing continues after cultivating, and continues to cultivate to the embryo of blastaea to still need to be again transferred in new Embryo Culture ware, because
It is different from the nutritional ingredient required for embryonic development in the 3rd day to the 6th day for first day to the 3rd day, it is necessary to the culture more renewed
Ware can just ensure that embryonic development is good.Therefore need to make substantial amounts of Embryo Culture ware daily, while also needing to ensure each
Different embryo mediums will not malfunction in individual Embryo Culture ware, and it is spiritual at work that this is just necessarily required to laboratory worker
The concentration of height is kept, this undoubtedly increases workload again, careless slightly to go wrong.
Lab assistant has the following disadvantages during the culture drop of culture dish is made:
1st, droplet size is different.The qualification that each lab assistant is grasped due to working experience and to laboratory tool
Difference, the laboratory culture dish of mark is different, and there is also difference for the culture drop size worked it out.This is possible to make
Embryo viability is influenceed into the difference for the culture environment that each embryo is contacted during Embryo Culture.
2nd, the amount of covering culture oil is also different.Due to when nutrient solution droplet is made, it is impossible to ensure that each is cultivated
Liquid droplet size all same, it is therefore desirable to which there is also difference for the culture oil of addition required for culture drop is completely covered.Different trainings
The growing environment of embryo can be influenceed by supporting the overlay capacity of oil, so as to influence Embryo viability.
The content of the invention
The technical problem to be solved in the present invention is:One kind is provided and ensure that every drop nutrient solution droplet size and covering are cultivated
The culture drop producing device of the essentially identical Embryo Culture ware of oily amount, can avoid human factor from being dripped in the culture of Embryo Culture ware
Error caused by manufacturing process, reduces the labor intensity of lab assistant, optimizes Embryo Culture environment, improves embryo's hair
Quality is educated, the problem of above-mentioned prior art is present is solved.
Solving the technical scheme of above-mentioned technical problem is:A kind of culture drop producing device of Embryo Culture ware, including work
Platform, push-rod electric machine, internal thread cylinder, external screw thread push rod, culture fluid cylinder, rotating disk, rotary tray motor and with culture fluid cylinder coordinate release training
The piston head of nutrient solution, described workbench includes work top, the installing plate installed in work top upper surface and is connected to work
Make the support feet of table top lower end, described push-rod electric machine is installed on a mounting board by connector I, described culture fluid cylinder bottom
Be additionally provided with drop outlets, culture fluid cylinder by connector II on a mounting board, the output shaft and interior spiral shell of described push-rod electric machine
Line cylinder connection, external screw thread push rod top is threadedly attached in internal thread cylinder, and external screw thread push rod lower end is connected simultaneously with piston head
In culture fluid cylinder, described rotary tray motor is arranged on work top, and rotating disk is driven by the output shaft of rotary tray motor to be revolved
Turn, rotating disk is located at below the drop outlets of culture fluid cylinder.
Described push-rod electric machine is also electrically connected with push rod time switch.
Described rotary tray motor is also connected with rotating disk time switch.
Described rotating disk is directly installed on the output shaft of rotary tray motor.
The culture drop producing device of the lid Embryo Culture ware also includes little gear, gear wheel and rotating shaft, described small tooth
Wheel is on the output shaft of rotary tray motor, and described gear wheel and rotating disk are arranged in rotating shaft, and rotating shaft passes through bearing and bearing
Seat is arranged on work top, and little gear is engaged with gear wheel.
Described installing plate top is additionally provided with support mounting circular ring, and protective cover is set with support mounting circular ring.
Due to using above-mentioned technical proposal, the invention has the advantages that:
1st, the present invention is to drive internal thread cylinder by push-rod electric machine, and internal thread cylinder drives external screw thread push rod to move downward again, so that
Nutrient solution of the extruding in culture fluid cylinder, makes nutrient solution be dripped to from drop outlets on rotating disk.By controlling push-rod electric machine to rotate
Angle fix, it becomes possible to ensure that the distance that each external screw thread push rod is moved downward is identical so that often drop culture drop size
It is identical.Because the size of culture drop is identical, therefore it is also identical that the oily consumption of the culture of culture drop is completely covered.Therefore use energy of the present invention
Enough amounts for ensureing often drop nutrient solution droplet size and covering culture oil are essentially identical, it is to avoid human factor is trained in Embryo Culture ware
The error caused during dripping is made is supported, Embryo Culture environment is optimized, improves embryonic development quality.
2nd, the labor intensity of lab assistant can be reduced using the present invention, the essence of substantial amounts of lab assistant can be liberated
Power goes other the related work of completion optimization auxiliary procreation technology, improves patient's curative effect and satisfaction.
3rd, the rotation and stopping of corresponding motor are controlled by using time switch, automation can be realized, training is greatly improved
Support the operating efficiency that dripping is made.
Below, the technical characteristic work of producing device is dripped in the culture in conjunction with the accompanying drawings and embodiments to the Embryo Culture ware of the present invention
Further instruction.
Brief description of the drawings
Fig. 1:The culture dripping of the Embryo Culture ware of the embodiment of the present invention 1 makees apparatus structure schematic diagram.
Fig. 2:The culture dripping of the Embryo Culture ware of the embodiment of the present invention 2 makees apparatus structure schematic diagram.
Fig. 3:The protective cover structural representation of the present invention.
In figure:1- push-rod electric machines, the output shaft of 101- push-rod electric machines, 2- internal threads cylinder, 3- external screw thread push rods, 4- pistons
Head, 5- culture fluid cylinders, 51- drop outlets, 6- rotating disks, 7- rotary tray motors, the output shaft of 71- rotary tray motors, 8- mounting seats, 9- works
Make platform, 91- installing plates, 92- work tops, 93- support feets, 10- connectors II, 11- connectors I, 12- push rod time switches,
13- rotating shafts, 14- gear wheels, 15- little gears, 16- rotating disk time switches, 17- bearing blocks, 18- support mounting circular rings, 19- protections
Cover.
Embodiment
Embodiment 1:A kind of culture drop producing device of Embryo Culture ware, as shown in Figure 1(Dress culture fluid cylinder is drawn as in Fig. 1
Transparent configuration, in order to see its internal structure clearly), including workbench 9, push-rod electric machine 1, internal thread cylinder 2, external screw thread push rod 3, training
Nutrient solution cylinder 5, rotating disk 6, rotary tray motor 7 and the piston head 4 for coordinating release nutrient solution with culture fluid cylinder 5, described workbench include
Work top 92, the installing plate 91 installed in work top upper surface and the support feet 93 for being connected to work top lower end, it is described
Push-rod electric machine 1 by connector I 11 be arranged on installing plate 91 on, the described bottom of culture fluid cylinder 5 is additionally provided with drop outlets
51, culture fluid cylinder 5 is by connector II 10 on installing plate 91, and the output shaft 101 of described push-rod electric machine connects with internal thread cylinder 2
Connect, the top of external screw thread push rod 3 is connected through a screw thread mode in internal thread cylinder 2, the lower end of external screw thread push rod 3 and piston head 4
Connect and be located in culture fluid cylinder 5, described rotary tray motor 7 is arranged on work top 92 by mounting seat 8, and rotating disk 6 is direct
On the output shaft 71 of rotary tray motor, driven and rotated by the output shaft 71 of rotary tray motor, rotating disk is located at culture fluid cylinder
The lower section of drop outlets 51.
The course of work of the present embodiment:Embryo Culture ware is placed on rotating disk 6, manually booted in push-rod electric machine 1 drives
Thread barrel 2 rotates certain time(Control the rotating speed of each push-rod electric machine identical with the working time, with ensure external screw thread push rod 3 to
The distance of lower motion is identical), external screw thread push rod 3 produced moved downward in internal thread 2 rotary courses of cylinder, so as to extrude mounted in training
Nutrient solution in nutrient solution cylinder 5, makes nutrient solution be dripped to from drop outlets 51 on rotating disk 6, completes the making of a culture drop.Then stop
Throw-out lever motor 1, manually boots rotary tray motor 7 and drives rotating disk 6 to rotate to an angle(Control the rotating speed and work of each rotary tray motor
Make that the time is identical, to ensure that the angle of turntable rotation is identical), stop rotary tray motor 7, then push-rod electric machine 1 is manually booted, in rotating disk
Another place continue make culture drop, so repeatedly until make on Embryo Culture ware all cultures drop.
Embodiment 2:A kind of culture drop producing device of Embryo Culture ware, as shown in Figure 2(Dress culture fluid cylinder is drawn as in Fig. 2
Transparent configuration, in order to see its internal structure clearly), including workbench 9, push-rod electric machine 1, internal thread cylinder 2, external screw thread push rod 3, training
Nutrient solution cylinder 5, rotating disk 6, rotary tray motor 7 and the piston head 4 for coordinating release nutrient solution with culture fluid cylinder 5, push-rod electric machine 1 are gone back and push rod
Time switch 12 is electrically connected, and rotary tray motor 7 is also connected with rotating disk time switch 16.Described workbench include work top 92,
Installing plate 91 and the support feet 93 for being connected to work top lower end, described push-rod electric machine 1 installed in work top upper surface
It is arranged on by connector I 11 on installing plate 91, the described bottom of culture fluid cylinder 5 is additionally provided with drop outlets 51, cultivates fluid cylinder 5
By connector II 10 on installing plate 91, the output shaft 101 of described push-rod electric machine is connected with internal thread cylinder 2, and external screw thread is pushed away
The top of bar 3 is connected through a screw thread mode in internal thread cylinder 2, and the lower end of external screw thread push rod 3 is connected with piston head 4 and positioned at training
In nutrient solution cylinder 5, described rotary tray motor 7 is arranged on work top 92 by mounting seat 8, and rotating disk is located at the drop of culture fluid cylinder
The lower section of outlet 51, rotating disk is driven by the output shaft 71 of rotary tray motor to be rotated, and its concrete structure is:The present embodiment also includes small
Gear 15, gear wheel 14 and rotating shaft 13, described little gear 15 are arranged on the output shaft 71 of rotary tray motor, described gear wheel
14 and rotating disk 6 be arranged in rotating shaft 13, rotating shaft is arranged on work top 92 by bearing and bearing block 17, little gear and canine tooth
Wheel engagement.
The course of work of the present embodiment:When setting the energization of push rod time switch 12 and rotating disk time switch 16 first
Between, making the working condition of push-rod electric machine 1 and rotary tray motor 7 is:The startup certain time of push-rod electric machine 1 makes external screw thread push rod 3 to train
Nutrient solution, which is expressed on rotating disk 6, forms culture drop, and now rotary tray motor 7 is in halted state, and then push-rod electric machine 1 stops, rotating disk
Electric motor starting certain time drive turntable rotation, then rotary tray motor stop, push-rod electric machine start, each rotary tray motor rotating speed and
The time of work is identical, to ensure that the distance that external screw thread push rod 3 is moved downward is identical, when the rotating speed of each push-rod electric machine and work
Between it is also identical, to ensure that the angle of turntable rotation is identical.So circulation, until making all cultures on Embryo Culture ware
Drop.
In various embodiments of the present invention, the described top of installing plate 91 is additionally provided with support mounting circular ring 18, is installed in support
Protective cover 19 is set with annulus, protective cover 19 can be by all component protections inside it.Protective cover 19 can be in support
Slided on mounting circular ring 18, the structure of similar curtain is deployable to draw in.Protective cover 19 is layer of transparent, the thickness of high light transmittance
Plastic jacket, the effect with protection Embryo Culture ware, injection culture drop and the covering culture oil in culture drop in culture dish bottom
During prevent from being polluted by bacterium, it is to avoid the waste of the nutrient solution caused by being contaminated, it also avoid because of culture
The seriously polluted situation for causing embryonic development to be obstructed of liquid occurs.
In various embodiments of the present invention, described connector I and connector II can be connecting rod or geometrical clamp, if even
Connection rod structure, then push-rod electric machine, culture fluid cylinder be fixedly mounted at it is non-dismountable on installing plate, it is if fixed folder structure, then solid
Clamp is arranged on installing plate, and push-rod electric machine, culture fluid cylinder are gripped by geometrical clamp, are dismountable structures.
In various embodiments of the present invention, culture fluid cylinder 5 can be made using transparent material, in order to observe its internal media
Situation.Certainly, culture fluid cylinder 5 can also be made using opaque material.
Time switch of the present invention can directly commercially, and the setting up procedure of time switch belongs to existing skill
Art, here is omitted.
Claims (6)
1. a kind of culture drop producing device of Embryo Culture ware, it is characterised in that:Including workbench(9), push-rod electric machine(1), it is interior
Thread barrel(2), external screw thread push rod(3), culture fluid cylinder(5), rotating disk(6), rotary tray motor(7)With with culture fluid cylinder(5)Cooperation is pushed away
Go out the piston head of nutrient solution(4), described workbench includes work top(92), installing plate installed in work top upper surface
(91)With the support feet for being connected to work top lower end(93), described push-rod electric machine(1)Pass through connector I(11)Installed in peace
Fill plate(91)On, described culture fluid cylinder(5)Bottom is additionally provided with drop outlets(51), cultivate fluid cylinder(5)Pass through connector II
(10)In installing plate(91)On, the output shaft of described push-rod electric machine(101)With internal thread cylinder(2)Connection, external screw thread push rod(3)
Top is threadedly attached in internal thread cylinder(2)It is interior, external screw thread push rod(3)Lower end and piston head(4)Connect and be located at nutrient solution
Cylinder(5)Interior, described rotary tray motor(7)Installed in work top(92)On, rotating disk(6)Pass through the output shaft of rotary tray motor(71)
Rotation is driven, rotating disk is located at the drop outlets of culture fluid cylinder(51)Lower section.
2. the culture drop producing device of Embryo Culture ware according to claim 1, it is characterised in that:Described push-rod electric machine
(1)Also with push rod time switch(12)Electrical connection.
3. the culture drop producing device of Embryo Culture ware according to claim 1, it is characterised in that:Described rotary tray motor
(7)Also with rotating disk time switch(16)Connection.
4. the culture drop producing device of the Embryo Culture ware according to claim 1,2 or 3, it is characterised in that:Described turns
Disk(6)It is directly installed on the output shaft of rotary tray motor(71)On.
5. the culture drop producing device of the Embryo Culture ware according to claim 1,2 or 3, it is characterised in that:Lid embryo
The culture drop producing device of culture dish also includes little gear(15), gear wheel(14)And rotating shaft(13), described little gear
(15)Installed in the output shaft of rotary tray motor(71)On, described gear wheel(14)And rotating disk(6)Installed in rotating shaft(13)On, turn
Axle passes through bearing and bearing block(17)Installed in work top(92)On, little gear is engaged with gear wheel.
6. the culture drop producing device of the Embryo Culture ware according to claim 1,2 or 3, it is characterised in that:Described peace
Fill plate(91)Top is additionally provided with support mounting circular ring(18), protective cover is set with support mounting circular ring(19).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201710153565.7A CN106995784A (en) | 2017-03-15 | 2017-03-15 | The culture drop producing device of Embryo Culture ware |
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CN201710153565.7A CN106995784A (en) | 2017-03-15 | 2017-03-15 | The culture drop producing device of Embryo Culture ware |
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CN201710153565.7A Pending CN106995784A (en) | 2017-03-15 | 2017-03-15 | The culture drop producing device of Embryo Culture ware |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108165524A (en) * | 2018-02-05 | 2018-06-15 | 苏州大学 | It is a kind of to tear the liquid droplet distribution system of egg and liquid droplet distribution method open for egg cell |
CN116953264A (en) * | 2023-07-24 | 2023-10-27 | 东蕴医疗科技(上海)有限公司 | Embryo culture liquid drop pH value measuring device |
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CN206586240U (en) * | 2017-03-15 | 2017-10-27 | 柳州市妇幼保健院 | Human assistance egg mother cell/embryo cryopreservation system |
CN207047250U (en) * | 2017-03-15 | 2018-02-27 | 柳州市妇幼保健院 | The culture drop producing device of Embryo Culture ware |
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2017
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GB1539807A (en) * | 1975-12-03 | 1979-02-07 | Burron Medical Prod Inc | Drop detector |
US6127125A (en) * | 1998-03-30 | 2000-10-03 | Hitachi Software Engineering Co., Ltd. | Sample application method and device |
US20030094502A1 (en) * | 2001-10-21 | 2003-05-22 | Per Andersson | Method and instrumentation for micro dispensation of droplets |
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CN108165524A (en) * | 2018-02-05 | 2018-06-15 | 苏州大学 | It is a kind of to tear the liquid droplet distribution system of egg and liquid droplet distribution method open for egg cell |
CN116953264A (en) * | 2023-07-24 | 2023-10-27 | 东蕴医疗科技(上海)有限公司 | Embryo culture liquid drop pH value measuring device |
CN116953264B (en) * | 2023-07-24 | 2024-02-06 | 东蕴医疗科技(上海)有限公司 | Embryo culture liquid drop pH value measuring device |
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Application publication date: 20170801 |