CN106994172A - A kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, pulvis and preparation method thereof - Google Patents
A kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, pulvis and preparation method thereof Download PDFInfo
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- CN106994172A CN106994172A CN201710439743.2A CN201710439743A CN106994172A CN 106994172 A CN106994172 A CN 106994172A CN 201710439743 A CN201710439743 A CN 201710439743A CN 106994172 A CN106994172 A CN 106994172A
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- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
The present invention relates to the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, pulvis and preparation method thereof, belong to technical field of traditional Chinese medicines.The Chinese medicine composition includes following raw material：6~8 parts of 3~5 parts of kuh-seng, 2~5 parts of the Radix Astragali, 5~8 parts of melanoidin malt, 10~15 parts of stone-like omphalia, 5~8 parts of the root of herbaceous peony, 10~14 parts of rhizome of cyrtomium, 2~5 parts of calamus, 2~6 parts of the tuber of stemona, 4~7 parts of fructus ulmi, 3~7 parts of dried orange peel, 10~12 parts of the root of kudzu vine, 3~6 parts of Radix Codonopsis and American Ginseng.The pulvis that the present invention is provided, is prepared by following raw material：The Chinese medicine composition 90%~95%, Glucurolactone 2%~6% and astaxanthin 1%~4%.The preparation method of the present invention is fermented using nanometer low temperature broken wall combination lactobacillus lactis, improves Chinese medicine rate of release, keeps pharmaceutical activity, final utilization rate improves 5 times, complex enzyme is added simultaneously to be digested, the attachment of enteron aisle microsporidian can be effectively reduced, and raising prevents and treats curative effect.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, and in particular to a kind of Chinese traditional medicine composition of prophylactic treatment gut of shrimp microsporidian
Thing, pulvis and preparation method thereof.
Background technology
Intestinal epithelial cell microsporidian mainly encroaches on the enteron aisle epidermis of prawn, and then infects hepatopancrease, causes prawn intestines
Road absorption function declines, serious appearance enteritis and hepatopancrease atrophy.Prawn infects intestinal epithelial cell microsporidian, most substantially
Feature be exactly that the speed of growth becomes slow, cause prawn individual difference big.But infect intestinal epithelial cell microsporidian
Shrimp, is not in death, and appetite is unaffected, can normal material feeding.
At present, it has not been found that the effective measures treated for gut of shrimp epithelial cell microsporidian, therefore, are carried out pre-
Anti- work is extremely important, for example, choose famous brand high-quality shrimp seedling, by strict detection, prevents seed from carrying；Finding the disease
Culturing area, changes water less as far as possible, as that need to change water, be by disinfecting；" clear water peace ", " vigor are commonly used in breeding process
Carbon ", " many stabilizing water qualities of the regulation such as II " of sharp bacterium, prevent water becomes from inducing morbidity；Spice feeds " LY- biogens+benefit essence+nutrition
Cable release " health care profit enteron aisle, strengthens immunity of prawn；Periodically use " Promised Land peace " to carry out changing bottom, improve pool bottom environment, can subtract
The growth of few sporozoite.But for suffering from the cultivating pool of gut of shrimp microsporidian, the long potential difference of prawn is often faced,
The problem of cost input is not directly proportional to profit reciprocation.
The content of the invention
In view of this, it is an object of the invention to provide a kind of Chinese traditional medicine composition of prophylactic treatment gut of shrimp microsporidian
Thing, the characteristics of being acted on potent desinsection, suppression worm.
The present invention also aims to provide a kind of pulvis of prophylactic treatment gut of shrimp microsporidian, have the pulvis
There is the characteristics of utilization rate is high to discharge quick with drug effect, while the autoimmunity of prawn can be effectively improved.
The present invention also aims to provide a kind of preparation method of the pulvis of prophylactic treatment gut of shrimp microsporidian, make
Methods described is easy to operate, with reproducible, the characteristics of cost is low.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The invention provides a kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, including following parts by weight
Raw material：3~5 parts of kuh-seng, 2~5 parts of the Radix Astragali, 5~8 parts of melanoidin malt, 10~15 parts of stone-like omphalia,
5~8 parts of the root of herbaceous peony, 10~14 parts of rhizome of cyrtomium, 2~5 parts of calamus, 2~6 parts of the tuber of stemona, 4~7 parts of fructus ulmi, 3~7 parts of dried orange peel,
6~8 parts of 10~12 parts of the root of kudzu vine, 3~6 parts of Radix Codonopsis and American Ginseng.
It is preferred that, include the raw material of following parts by weight：4 parts of kuh-seng, 3 parts of the Radix Astragali, 7 parts of melanoidin malt, 13 parts of stone-like omphalia, the root of herbaceous peony 6
7 parts of part, 12 parts of rhizome of cyrtomium, 3 parts of calamus, 4 parts of the tuber of stemona, 5 parts of fructus ulmi, 5 parts of dried orange peel, 11 parts of the root of kudzu vine, 5 parts of Radix Codonopsis and American Ginseng.
The invention provides a kind of pulvis of prophylactic treatment gut of shrimp microsporidian, by the original of following weight/mass percentage composition
Material is prepared：
Described Chinese medicine composition 90%~95%
Glucurolactone 2%~6%
Astaxanthin 1%~4%.
It is preferred that, prepared by the raw material of following weight/mass percentage composition：
Described Chinese medicine composition 94%
Glucurolactone 4%
Astaxanthin 2%.
Present invention also offers the preparation method of the pulvis of described prophylactic treatment gut of shrimp microsporidian, including it is following
Step：
1) described traditional Chinese medicine composite is crushed to 600~1000 mesh, obtains herb powder；
2) to the step 1) obtained herb powder inoculating lactic acid lactobacillus fermented, obtained tunning drying
Processing；
3) by the step 2) obtained tunning digested under compound enzyme effect, obtains enzymolysis product；It is described
Complex enzyme includes the enzyme of following percentage composition：Zytase 15~22%, pectase 8~12%, neutral proteinase 20~25%,
Papain 3~5%, cellulase 15~25%, galactosidase 2~5% and lipase 10~18%；
4) enzymolysis product is mixed with Glucurolactone and astaxanthin, obtains the pulvis of gut of shrimp microsporidian.
It is preferred that, the lyophilized lactobacillus lactis inoculum concentration is 10~10,000,000,000 CFU/g.
It is preferred that, the step 2) fermentation type be anaerobic fermentation；The time of the fermentation is 5~8 days；The hair
Ferment temperature is 20~35 DEG C.
It is preferred that, the vigor of various enzymes is as follows in the complex enzyme：3000~50000IU/g of zytase, pectase
25000~30000IU/g, 35000~50000IU/g of neutral proteinase, 8000~1000IU/g of papain, cellulase
150000~200000U/g, 16000~20000U/g of 3500~5000IU/g of galactosidase and lipase.
It is preferred that, the temperature of enzymolysis is 30~35 DEG C；The time of enzymolysis is 10~20min.
It is preferred that, the step 2) in ferment before moisture is added into herb powder；During the quality of the moisture addition is accounted for
Medicine powder quality 38~40%.
The invention provides a kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, including following percentage composition
Raw material：3~5 parts of kuh-seng, 2-5 parts of the Radix Astragali, 5~8 parts of melanoidin malt, 10~15 parts of stone-like omphalia, 5~8 parts of the root of herbaceous peony, rhizome of cyrtomium 10~14
Part, 2~5 parts of calamus, 2~6 parts of the tuber of stemona, 4~7 parts of fructus ulmi, 3~7 parts of dried orange peel, 10~12 parts of the root of kudzu vine, 3~6 parts of Radix Codonopsis and the West
6~8 parts of ginseng.The effect of kuh-seng is desinsection expelling parasite in the present invention, but intestinal tissue can be damaged, and other products are mainly
Booster action, spleen benefiting and stimulating the appetite protects the effect such as liver pancreas.The present invention is played by the way that 13 kinds of Chinese medicines are compounded so as to play collaboration
The effect of drug effect.
The invention provides a kind of pulvis of prophylactic treatment gut of shrimp microsporidian, by the original of following weight/mass percentage composition
Material is prepared：Described Chinese medicine composition 90%~95%, Glucurolactone 2%~6% and astaxanthin 1%~4%.
Chinese medicine composition collaboration of the present invention plays the drug effect of desinsection expelling parasite, while Glucurolactone protection liver pancreas is added,
Reducing medicine stress；Natural astaxanthin is added, is conducive to improving the autoimmunity of prawn.The prophylactic treatment pair that the present invention is provided
The pulvis of shrimp enteron aisle microsporidian can effectively prevent and treat prawn infection enteron aisle microsporidian disease.
The preparation method of the pulvis for the prophylactic treatment gut of shrimp microsporidian that the present invention is provided, comprises the following steps：1)
Described traditional Chinese medicine composite is crushed to 600~1000 mesh, herb powder is obtained；2) to the step 1) obtained Chinese medicine
Powder inoculating lactic acid lactobacillus is fermented, and obtains tunning；3) by the step 2) obtained tunning is in complex enzyme
Effect is lower to be digested, and obtains enzymolysis product；The complex enzyme includes the enzyme of following percentage composition：Zytase 20%, pectin
Enzyme 10%, neutral proteinase 25%, papain 5%, cellulase 20%, galactosidase 5% and lipase 15%；4)
Enzymolysis product is mixed with Glucurolactone and astaxanthin, the pulvis of gut of shrimp microsporidian is obtained.The system of the present invention
Preparation Method, combines lyophilized lactobacillus lactis fermentation using nanometer low temperature broken wall, improves Chinese medicine rate of release, while keeping medicine
Activity, final utilization rate improves 5 times, is digested while adding complex enzyme, can effectively reduce the attached of enteron aisle microsporidian
, improve and prevent and treat curative effect.
Embodiment
The invention provides a kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, including following parts by weight
Raw material：3~5 parts of kuh-seng, 2~5 parts of the Radix Astragali, 5~8 parts of melanoidin malt, 10~15 parts of stone-like omphalia, 5~8 parts of the root of herbaceous peony, 10~14 parts of rhizome of cyrtomium,
2~5 parts of calamus, 2~6 parts of the tuber of stemona, 4~7 parts of fructus ulmi, 3~7 parts of dried orange peel, 10~12 parts of the root of kudzu vine, 3~6 parts of Radix Codonopsis and American Ginseng 6
~8 parts.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes kuh-seng.By weight
Meter, the kuh-seng is 3~5 parts, more preferably 4 parts.The kuh-seng has effects that desinsection expelling parasite.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes the Radix Astragali.By weight
Meter, the Radix Astragali is 2~5 parts, more preferably 3 parts.The Radix Astragali is rich in astragalus polyose, with the work for improving immunity of prawn
With.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes melanoidin malt.By weight
Part meter, the melanoidin malt is 5~8 parts, more preferably 7 parts.The melanoidin malt has the effect for promoting digestion.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes stone-like omphalia.By weight
Meter, the stone-like omphalia is 10~15 parts, more preferably 13 parts.The stone-like omphalia has expelling parasite and the effect of disperse accumulations.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes the root of herbaceous peony.By weight
Meter, the root of herbaceous peony is 5~8 parts, more preferably 6 parts.There is the root of herbaceous peony protection liver pancreas to use.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes rhizome of cyrtomium.By weight
Meter, the rhizome of cyrtomium is 10~14 parts, more preferably 12 parts.The rhizome of cyrtomium has anthelmintic action.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes calamus.By weight
Meter, the calamus is 2~5 parts, more preferably 3 parts.The calamus has the effect of auxiliary expelling parasite.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes the tuber of stemona.By weight
Meter, the tuber of stemona is 2~6 parts, more preferably 4 parts.The tuber of stemona has the effect of auxiliary expelling parasite.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes fructus ulmi.By weight
Meter, the fructus ulmi is 4~7 parts, more preferably 5 parts.The fructus ulmi has expelling parasite and promotes the effect of digestion.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes dried orange peel.By weight
Meter, the dried orange peel is 3~7 parts, more preferably 5 parts.The dried orange peel has the effect for promoting digestion and absorbing.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes the root of kudzu vine.By weight
Meter, the root of kudzu vine is 10~12 parts, more preferably 11 parts.The root of kudzu vine has the work for improving immunity of prawn and promoting digestion
With.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes Radix Codonopsis.By weight
Meter, the Radix Codonopsis is 3~6 parts, more preferably 5 parts.The Radix Codonopsis has the effect of protection prawn liver pancreas.
The raw material of the Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian of the present invention includes American Ginseng.By weight
Part meter, the American Ginseng is 6~8 parts, more preferably 7 parts.The American Ginseng has the effect for improving immunity of prawn.
In the present invention, kuh-seng, rhizome of cyrtomium and stone-like omphalia are monarchs drug in a prescription in the 13 taste Chinese medicine, rise and mainly drive the micro- spore of gut of shrimp
The effect of worm；Calamus, fructus ulmi and the tuber of stemona are ministerial drugs, play auxiliary and drive gut of shrimp microsporidian；The Radix Astragali, Radix Codonopsis, west
American ginseng and the root of herbaceous peony are adjutant, play restriction drug toxicity, improve the effect of immunity of prawn and protection prawn liver pancreas；Melanoidin malt,
Dried orange peel and the root of kudzu vine play a part of improving medicine digestion to make medicine.
In the present invention, the preparation method of the Chinese medicine composition comprises the following steps：
A. such scheme is obtained into Chinese medicine composition to crush, obtains herb powder；
B. the herb powder obtained in the step A is mixed with water, extracts, obtain medicinal plant extraction；
C. by the medicinal plant extraction obtained in the step B through filtering, filtrate is collected, Chinese medicine composition is obtained.
In the present invention, the method for the crushing is not particularly limited, using crushing side well-known to those skilled in the art
Method.The Chinese medicine composition particle diameter of the crushing is preferably 600~1000 mesh.
In the present invention, the quality of the herb powder and the volume ratio of water are 1mg：10~20ml, more preferably 1mg：
15ml。
In the present invention, the method for the extraction is not particularly limited, using extraction side well-known to those skilled in the art
Method.The temperature of the extraction is preferably 60 DEG C~80 DEG C.
In the present invention, the method for the filtering is not particularly limited, using filtering side well-known to those skilled in the art
Method.The aperture of the filtering is preferably 600~1000 mesh.
In the present invention, further preferably concentrated, dried including the filtrate after the filtering, obtain Chinese medicine composition.
The method of the concentration is using the method being concentrated in vacuo, using vacuum concentration side well-known to those skilled in the art
Method.The temperature of the concentration is preferably 60 DEG C~80 DEG C, more preferably 70 DEG C；The time of the concentration is preferably 10min
~30min, more preferably 20min.The temperature of the drying is preferably 50 DEG C~80 DEG C, more preferably 65 DEG C.The drying
Degree is preferably that herb powder water content is not higher than 5%.
The invention provides a kind of pulvis of prophylactic treatment gut of shrimp microsporidian, by the original of following weight/mass percentage composition
Material is prepared：
Described Chinese medicine composition 90%~95%
Glucurolactone 2%~6%
Astaxanthin 1%~4%.
The raw material of the pulvis for the prophylactic treatment gut of shrimp microsporidian that the present invention is provided includes described Chinese medicine composition.
It is 100% meter according to raw material gross mass, the mass percent of the Chinese medicine composition is 90%~95%, more preferably 94%.
The raw material of the pulvis for the prophylactic treatment gut of shrimp microsporidian that the present invention is provided includes Glucurolactone.Press
It is 100% meter according to raw material gross mass, the mass percent of the Glucurolactone is 2%~6%, more preferably 4%.This
In invention, the source of the Glucurolactone is not particularly limited, using glucose well-known to those skilled in the art
Aldehydic acid lactones.
The raw material of the pulvis for the prophylactic treatment gut of shrimp microsporidian that the present invention is provided includes astaxanthin.It is total according to raw material
Quality is 100% meter, and the mass percent of the astaxanthin is 1%~4%, more preferably 2%.In the present invention, the shrimp is blue or green
The source of element is not particularly limited, using astaxanthin well-known to those skilled in the art.The astaxanthin is preferably day
Right left-handed r ' r- astaxanthins.
Present invention also offers the preparation method of the pulvis of the prophylactic treatment gut of shrimp microsporidian, including following step
Suddenly：
1) described traditional Chinese medicine composite is crushed to 600~1000 mesh, obtains herb powder；
2) to the step 1) inoculating lactic acid lactobacillus is fermented in obtained herb powder, and obtained tunning enters
Row drying and processing；
3) by the step 2) obtained tunning digested under compound enzyme effect, obtains enzymolysis product；It is described
Complex enzyme includes the enzyme of following percentage composition：Zytase 15~22%, pectase 8~12%, neutral proteinase 20~25%,
Papain 3~5%, cellulase 15~25%, galactosidase 2~5% and lipase 10~18%；
4) enzymolysis product is mixed with Glucurolactone and astaxanthin, obtains the powder of gut of shrimp microsporidian
Agent.
The traditional Chinese medicine composite is crushed to 600~1000 mesh by the present invention, obtains herb powder.
In the present invention, the method for the crushing is not particularly limited, using crushing well-known to those skilled in the art
Method.The particle diameter of the traditional Chinese medicine composite is preferably 800 mesh.
In the present invention, the method that the Chinese traditional medicine composition is crushed includes remixing after the raw material is crushed respectively, Huo Zhexian
Crushed again after raw material is mixed, obtain herb powder.
The present invention is fermented to the herb powder inoculating lactic acid lactobacillus, obtains tunning.
In the present invention, the lactobacillus lactis inoculum concentration is preferably 10~10,000,000,000 CFU/g herb powders, more preferably 50
Hundred million CFU/g herb powders.The method of the inoculation is not particularly limited, using inoculation method well-known to those skilled in the art
.
In the present invention, the type of the fermentation is preferably anaerobic fermentation；The method of the anaerobic fermentation is not particularly limited,
Using anaerobic fermentation method well-known to those skilled in the art.
In the present invention, the time of the fermentation is preferably 5~8 days, more preferably 7 days.The fermentation temperature is preferably 20
~35 DEG C, more preferably 25~32 DEG C.
In the present invention, lactobacillus lactis can produce aromatic substance and immune lactic acid bacteria fragment, Ke Yiti while fermentation
The palatability of high feed, improves the active immunity level of shrimp body.
In the present invention, moisture is preferably added into herb powder before fermentation.The quality of the moisture addition preferably accounts for Chinese medicine
The 38~40% of powder quality, more preferably 39%.
In the present invention, preferred pair tunning carries out drying and processing after fermentation ends.The temperature of the drying is preferably 28
~32 DEG C, more preferably 30 DEG C.The drying terminates when being not higher than 20% to moisture.
Obtain after tunning, the present invention is digested the tunning under compound enzyme effect, obtain enzymolysis production
Thing；The complex enzyme includes the enzyme of following percentage composition：Zytase 15~22%, pectase 8~12%, neutral proteinase 20
~25%, papain 3~5%, cellulase 15~25%, galactosidase 2~5% and lipase 10~18%.
In the present invention, the complex enzyme preferably includes the enzyme of following percentage composition：Zytase 20%, pectase 10%,
Neutral proteinase 25%, papain 5%, cellulase 20%, galactosidase 5% and lipase 15%.The present invention is right
The source of the complex enzyme is not particularly limited, using the source of complex enzyme well-known to those skilled in the art.It is described
The vigor of various enzymes is preferably as follows in complex enzyme：3000~50000IU/g of zytase, 25000~30000IU/g of pectase,
35000~50000IU/g of neutral proteinase, 8000~1000IU/g of papain, 150000~200000U/ of cellulase
G, more preferably 16000~20000U/g of 3500~5000IU/g of galactosidase and lipase, zytase 50000U/g, fruit
Glue enzyme 30000U/g, neutral proteinase 50000U/g, papain 1000U/g, cellulase 200000U/g, galactoside
Enzyme 5000U/g and lipase 20000U/g.
In the present invention, the mass ratio of the tunning and complex enzyme is 1~150：1~250 more preferably 200.
Obtain after enzymolysis product, the present invention mixes the enzymolysis product with Glucurolactone and astaxanthin, obtains
The pulvis of gut of shrimp microsporidian.
In the present invention, the method for the mixing is not particularly limited, using mixing side well-known to those skilled in the art
Method.
Chinese medicine composition, pulvis or the side for the described prophylactic treatment gut of shrimp microsporidian that the present invention is provided
The application of pulvis prepared by method in the medicine for preparing prophylactic treatment gut of shrimp microsporidian.
Arranged with reference to several groups of control experiment cases to a kind of prophylactic treatment gut of shrimp microsporidian of the invention provided
Chinese medicine composition, pulvis and preparation method thereof are described in detail, but they can not be interpreted as to present invention protection model
The restriction enclosed.
Embodiment 1
Weigh the Chinese medicine of following weight, kuh-seng 3g, Radix Astragali 5g, melanoidin malt 5g, stone-like omphalia 15g, root of herbaceous peony 5g, rhizome of cyrtomium 14g, calamus
2g, tuber of stemona 6g, fructus ulmi 4g, dried orange peel 7g, root of kudzu vine 10g, Radix Codonopsis 6g and American Ginseng 6g.Above-mentioned composition is crushed to 800 mesh, obtained
Herb powder；Herb powder is with water according to 1mg：10ml is mixed, and is extracted 2h under the conditions of 70 DEG C, is obtained medicinal plant extraction；Leaching liquor
Through filtering, filtrate is collected, filtrate concentrates 20min under the conditions of under drawing dummy status 60 DEG C, obtains condensed cream, through 65 DEG C of dryings,
Obtain Chinese medicine composition.
Embodiment 2
Weigh the Chinese medicine of following weight, kuh-seng 5g, Radix Astragali 2g, melanoidin malt 8g, stone-like omphalia 10g, root of herbaceous peony 8g, rhizome of cyrtomium 14g, calamus
2g, tuber of stemona 6g, fructus ulmi 4g, dried orange peel 7g, root of kudzu vine 10g, Radix Codonopsis 6g and American Ginseng 6g.Above-mentioned composition is crushed to 800 mesh, obtained
Herb powder；Herb powder is with water according to 1mg：10ml is mixed, and is extracted 2h under the conditions of 80 DEG C, is obtained medicinal plant extraction；Leaching liquor
Through filtering, filtrate is collected, filtrate concentrates 20min under the conditions of under drawing dummy status 70 DEG C, obtains condensed cream, through 65 DEG C of dryings,
Obtain Chinese medicine composition.
Embodiment 3
Weigh the Chinese medicine of following weight, kuh-seng 4g, Radix Astragali 3g, melanoidin malt 7g, stone-like omphalia 13g, root of herbaceous peony 6g, rhizome of cyrtomium 12g, calamus
3g, tuber of stemona 4g, fructus ulmi 5g, dried orange peel 5g, root of kudzu vine 11g, Radix Codonopsis 5g and American Ginseng 7g.Above-mentioned composition is crushed to 1600 mesh, obtained
To herb powder；Herb powder is with water according to 1mg：10ml is mixed, and is extracted 2h under the conditions of 80 DEG C, is obtained medicinal plant extraction；Extraction
Liquid collects filtrate through filtering, and filtrate is concentrating 20min under the conditions of under drawing dummy status 70 DEG C, obtaining condensed cream, dry through 65 DEG C
It is dry, obtain Chinese medicine composition.
Chinese medicine composition prepared by embodiment 1~3 dissolves the decoction to form that mass concentration is 1%, the decoction
Volume and the quality of prawn feed are 3ml according to volume mass ratio：7g ratio is mixed, and morbidity prawn is fed daily
Once, continuous feeding 7 days is interrupted 7 days food, then continuous feeding carries out the treatment of a course for the treatment of for 7 days.
Treatment results show, after the treatment end of a course for the treatment of, and the effect of embodiment 1 and embodiment 2 does not have notable difference,
To the killing rate of gut of shrimp microsporidian between 62~70%；Chinese medicine composition prepared by embodiment 3 is micro- to gut of shrimp
The killing rate of sporozoite is between 55~65%, and effect is slightly poor compared with Examples 1 and 2.
Embodiment 4
Weigh the Chinese medicine of 2 parts of following weight of identical, kuh-seng 3g, Radix Astragali 5g, melanoidin malt 5g, stone-like omphalia 15g, root of herbaceous peony 5g, pass through
Many 14g, calamus 2g, tuber of stemona 6g, fructus ulmi 4g, dried orange peel 7g, root of kudzu vine 10g, Radix Codonopsis 6g and American Ginseng 6g.
600 mesh are crushed to after above-mentioned two parts of Chinese medicine material is mixed, herb powder is obtained；Add into two parts of herb powders
Add water point, the quality of the moisture addition preferably accounts for the 38% of herb powder quality.It is inoculated with into described two parts of herb powders
Lactobacillus lactis carries out anaerobic fermentation, and one of which uses the fermentation technique of nanometer low temperature, and fermentation time is 5 days, fermentation temperature
For 20 DEG C；Another group is fermented under normal temperature condition, specifically, fermentation time is 5 days, fermentation temperature is 35 DEG C.Obtain
Two groups of tunnings all carry out drying and processing, moisture is not higher than 20%；The lactobacillus lactis inoculum concentration is 1,000,000,000
CFU/g herb powders；Two groups of described tunnings are subjected to enzymolysis 15 minutes at 30 DEG C under compound enzyme effect, two are obtained
Group enzymolysis product；The complex enzyme includes the enzyme of following percentage composition：Zytase 22%, pectase 8%, neutral proteinase
25%th, papain 3%, cellulase 25%, galactosidase 2% and lipase 15%；Various enzymes in the complex enzyme
Vigor be preferably as follows：Zytase 50000U/g, pectase 30000U/g, neutral proteinase 50000U/g, papain
1000U/g, cellulase 200000U/g, galactosidase 5000U/g and lipase 20000U/g.By described two groups of enzymolysis
Product is 90% according to above-mentioned Chinese medicine composition, and Glucurolactone is 6%, and astaxanthin is 4% mixing, obtains gut of shrimp
The pulvis of microsporidian.
By the pulvis and prawn feed of above-mentioned preparation according to 3g:1000g ratio is mixed, every to morbidity prawn
Once, continuous feeding 7 days is interrupted 7 days for day feeding, then continuous feeding carries out the treatment of a course for the treatment of for 7 days.Experiment effect shows：
Under conditions of later stage is using same process and zymolysis technique, the micro- spore of gut of shrimp prepared using the processing method of nanometer low temperature
Worm pulvis drug effect is better than the gut of shrimp microsporidian pulvis prepared under the conditions of 35 DEG C.Using the processing method system of nanometer low temperature
Standby gut of shrimp microsporidian pulvis to the killing rate of gut of shrimp microsporidian between 65~82%, the death rate of prawn
Between 4~10%；And the gut of shrimp microsporidian pulvis prepared under the conditions of 35 DEG C is killed to gut of shrimp microsporidian
Worm rate is substantially low, between 55~68%, and the death rate of prawn is between 6~15%.
Embodiment 5
Weigh the Chinese medicine of two parts of following weight of identical, kuh-seng 5g, Radix Astragali 2g, melanoidin malt 8g, stone-like omphalia 10g, the root of herbaceous peony 5~
8g, rhizome of cyrtomium 14g, calamus 2g, tuber of stemona 6g, fructus ulmi 4g, dried orange peel 7g, root of kudzu vine 10g, Radix Codonopsis 6g and American Ginseng 6g.
1000 mesh are crushed to after above-mentioned two parts of Chinese medicine material is mixed, herb powder is obtained；Into two parts of herb powders
Moisture is added, the quality of the moisture addition preferably accounts for the 40% of herb powder quality.Connect into two parts of the herb powder
Plant lactobacillus lactis and carry out anaerobic fermentation, fermentation time is 8 days, and fermentation temperature is 20 DEG C, and obtained tunning is dried
Processing, makes moisture be not higher than 20%；The lactobacillus lactis inoculum concentration is 10,000,000,000 CFU/g herb powders；By described two parts
Tunning carry out digesting 15 minutes at 35 DEG C under compound enzyme effect, obtain enzymolysis product；The complex enzyme include with
The enzyme of lower percentage composition：Zytase 20%, pectase 12%, neutral proteinase 20%, papain 5%, cellulase
20%th, galactosidase 5% and lipase 18%；The vigor of various enzymes is preferably as follows in the complex enzyme：Zytase
50000U/g, pectase 30000U/g, neutral proteinase 50000U/g, papain 1000U/g, cellulase 200000U/
G, galactosidase 5000U/g and lipase 20000U/g.By enzymolysis product described in a copy of it according to above-mentioned Chinese medicine composition
For 95%, Glucurolactone is 2%, and astaxanthin is 3% mixing, obtains the pulvis of gut of shrimp microsporidian；Other one
Part is without Glucurolactone and astaxanthin.The pulvis of above-mentioned preparation is prevented and treated using the application process of embodiment 4.
By the pulvis and prawn feed of above-mentioned preparation according to 3g:1000g ratio is mixed, every to morbidity prawn
Once, continuous feeding 7 days is interrupted 7 days for day feeding, then continuous feeding carries out the treatment of a course for the treatment of for 7 days.Experiment effect shows：
The pulvis drug effect that with the addition of gut of shrimp microsporidian made from Glucurolactone and astaxanthin is better than without grape
The pulvis of gut of shrimp microsporidian made from uronic acid lactones and astaxanthin.It with the addition of Glucurolactone and astaxanthin system
Gut of shrimp microsporidian pulvis to the killing rate of gut of shrimp microsporidian between 69~86%, the death of prawn
Rate is between 3~8%；And without gut of shrimp microsporidian made from Glucurolactone and astaxanthin pulvis to right
The killing rate of shrimp enteron aisle microsporidian is between 55~68%, and the death rate of prawn is between 18~42%.
Embodiment 6
Weigh the raw material of two parts of following weight of identical：Kuh-seng 4g, Radix Astragali 3g, melanoidin malt 7g, stone-like omphalia 13g, root of herbaceous peony 6g, pass through
Many 12g, calamus 3g, tuber of stemona 4g, fructus ulmi 5g, dried orange peel 5g, root of kudzu vine 11g, Radix Codonopsis 5g and American Ginseng 7g.
800 mesh are crushed to after above-mentioned two parts of Chinese medicine material is mixed, herb powder is obtained；Add into two parts of herb powders
Add water point, the quality of the moisture addition preferably accounts for the 39% of herb powder quality.Breast is inoculated with into two parts of herb powders
Lactobacillus lactis carries out anaerobic fermentation, and fermentation time is 6 days, and fermentation temperature is 25 DEG C, and obtained tunning drying and processing makes water
Point content is not higher than 20%；The lactobacillus lactis inoculum concentration is 5,000,000,000 CFU/g herb powders；By production of being fermented described in one of which
Thing carries out digesting 20 minutes at 30 DEG C under compound enzyme effect, obtains enzymolysis product；Tunning does not enter described in another set
The technique of row enzymolysis.The complex enzyme includes the enzyme of following percentage composition：Zytase 20%, pectase 10%, neutral protein
Enzyme 25%, papain 5%, cellulase 20%, galactosidase 5% and lipase 15%；It is various in the complex enzyme
The vigor of enzyme is as follows：Zytase 50000U/g, pectase 30000U/g, neutral proteinase 50000U/g, papain
1000U/g, cellulase 200000U/g, galactosidase 5000U/g and lipase 20000U/g.By the enzymolysis product and
Tunning is 93% according to above-mentioned Chinese medicine composition, and Glucurolactone is 3%, and astaxanthin is 2% mixing, obtains prawn
The pulvis of enteron aisle microsporidian.The pulvis of above-mentioned preparation is prevented and treated using the application process of embodiment 4.
By the pulvis and prawn feed of above-mentioned preparation according to 3g:1000g ratio is mixed, every to morbidity prawn
Once, continuous feeding 7 days is interrupted 7 days for day feeding, then continuous feeding carries out the treatment of a course for the treatment of for 7 days.Experiment effect shows：
The pulvis drug effect of gut of shrimp microsporidian made from being digested is better than without the micro- spore of the obtained gut of shrimp of enzymolysis
The pulvis of worm.The pulvis of gut of shrimp microsporidian made from being digested to the killing rate of gut of shrimp microsporidian 72~
Between 88%, the death rate of prawn is between 3~6%；And without the pulvis pair of the obtained gut of shrimp microsporidian of enzymolysis
The killing rate of gut of shrimp microsporidian is between 52~75%, and the death rate of prawn is between 5~12%.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Chinese medicine composition for preventing and treating gut of shrimp microsporidian, includes the raw material of following parts by weight：Kuh-seng 3~
5 parts, 2~5 parts of the Radix Astragali, 5~8 parts of melanoidin malt, 10~15 parts of stone-like omphalia, 5~8 parts of the root of herbaceous peony, 10~14 parts of rhizome of cyrtomium, 2~5 parts of calamus,
6~8 parts of 2~6 parts of the tuber of stemona, 4~7 parts of fructus ulmi, 3~7 parts of dried orange peel, 10~12 parts of the root of kudzu vine, 3~6 parts of Radix Codonopsis and American Ginseng.
2. Chinese medicine composition according to claim 1, it is characterised in that include the raw material of following parts by weight：4 parts of kuh-seng, Huang
3 parts of stilbene, 7 parts of melanoidin malt, 13 parts of stone-like omphalia, 6 parts of the root of herbaceous peony, 12 parts of rhizome of cyrtomium, 3 parts of calamus, 4 parts of the tuber of stemona, 5 parts of fructus ulmi, 5 parts of dried orange peel, Pueraria lobota
7 parts of 11 parts of root, 5 parts of Radix Codonopsis and American Ginseng.
3. a kind of pulvis for preventing and treating gut of shrimp microsporidian, it is characterised in that by the original of following weight/mass percentage composition
Material is prepared：
Chinese medicine composition 90%~95% described in claim 1 or 2
Glucurolactone 2%~6%
Astaxanthin 1%~4%.
4. pulvis according to claim 3, it is characterised in that prepared by the raw material of following weight/mass percentage composition：
Described Chinese medicine composition 94%
Glucurolactone 4%
Astaxanthin 2%.
5. the preparation method of pulvis described in claim 3, it is characterised in that comprise the following steps：
1) traditional Chinese medicine composite described in claim 1 or 2 is crushed to 600~1000 mesh, obtains herb powder；
2) to the step 1) obtained herb powder inoculating lactic acid lactobacillus fermented, at obtained tunning drying
Reason；
3) by the step 2) obtained tunning digested under compound enzyme effect, obtains enzymolysis product；It is described compound
Enzyme includes the enzyme of following percentage composition：Zytase 15~22%, pectase 8~12%, neutral proteinase 20~25%, pawpaw
Protease 3~5%, cellulase 15~25%, galactosidase 2~5% and lipase 10~18%；
4) enzymolysis product is mixed with Glucurolactone and astaxanthin, obtains the pulvis of gut of shrimp microsporidian.
6. preparation method according to claim 5, it is characterised in that the lactobacillus lactis inoculum concentration is 10~10,000,000,000
CFU/g。
7. preparation method according to claim 5, it is characterised in that the step 2) the type of fermentation be anaerobism hair
Ferment；The time of the fermentation is 5~8 days；The fermentation temperature is 20~35 DEG C.
8. preparation method according to claim 5, it is characterised in that the vigor of various enzymes is as follows in the complex enzyme：Wood
3000~50000IU/g of dextranase, 25000~30000IU/g of pectase, 35000~50000IU/g of neutral proteinase, pawpaw
000~1000IU/g of proteinase 8,150000~200000U/g of cellulase, 3500~5000IU/g of galactosidase and fat
16000~20000U/g of enzyme.
9. preparation method according to claim 5, it is characterised in that the temperature of enzymolysis is 30-35 degrees Celsius；Enzymolysis when
Between be 10~20min.
10. preparation method according to claim 5, it is characterised in that the step 2) in before fermentation into herb powder
Add moisture；The quality of the moisture addition accounts for herb powder quality 38~40%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582919A (en) * | 2017-10-23 | 2018-01-16 | 天津瑞贝特科技发展有限公司 | Treat Chinese medicine composition of shrimp enteron aisle sporidiosis and preparation method thereof |
| CN112790128A (en) * | 2020-12-31 | 2021-05-14 | 亚太海洋生物科技(厦门)有限公司 | Method for preventing Vertically spreading of liver and intestinal sporozoon of Litopenaeus vannamei |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987162A (en) * | 2012-12-25 | 2013-03-27 | 广东粤海饲料集团有限公司 | Prawn chinese herbal medicine feed additive and preparation method thereof |
| CN105561099A (en) * | 2016-02-18 | 2016-05-11 | 荣成市渔业技术推广站 | Traditional Chinese medicine composition for controlling litopenaeus-vannamei enterohepatic sporozoosis and control method thereof |
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- 2017-06-12 CN CN201710439743.2A patent/CN106994172B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987162A (en) * | 2012-12-25 | 2013-03-27 | 广东粤海饲料集团有限公司 | Prawn chinese herbal medicine feed additive and preparation method thereof |
| CN105561099A (en) * | 2016-02-18 | 2016-05-11 | 荣成市渔业技术推广站 | Traditional Chinese medicine composition for controlling litopenaeus-vannamei enterohepatic sporozoosis and control method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582919A (en) * | 2017-10-23 | 2018-01-16 | 天津瑞贝特科技发展有限公司 | Treat Chinese medicine composition of shrimp enteron aisle sporidiosis and preparation method thereof |
| CN112790128A (en) * | 2020-12-31 | 2021-05-14 | 亚太海洋生物科技(厦门)有限公司 | Method for preventing Vertically spreading of liver and intestinal sporozoon of Litopenaeus vannamei |
| CN112790128B (en) * | 2020-12-31 | 2022-06-10 | 亚太海洋生物科技(厦门)有限公司 | Method for preventing Vertically spreading of liver and intestinal sporozoon of Litopenaeus vannamei |
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Effective date of registration: 20220302 Address after: 361000 room 419d, Chuangye building, No. 11-1, Huoju East Road, Chuangye Park, torch high tech Zone, Xiamen, Fujian Patentee after: Haiyi (Xiamen) Aquatic Technology Co.,Ltd. Address before: 361000 room 519a, entrepreneurship building, entrepreneurship Park, torch high tech Zone, Xiamen, Fujian Patentee before: HAIYI (XIAMEN) BIOLOGICAL TECHNOLOGY CO.,LTD. |
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