CN106987522B - The hot lid arrangement and control method of gene-amplificative instrament - Google Patents
The hot lid arrangement and control method of gene-amplificative instrament Download PDFInfo
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- 238000001514 detection method Methods 0.000 claims abstract description 21
- 238000003825 pressing Methods 0.000 claims abstract description 14
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- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 7
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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Abstract
The invention discloses the hot lid arrangement and control method of a kind of gene-amplificative instrament, hot lid arrangement includes the hot lid pressing plate from top to bottom set gradually, hot lid heat insulating mattress, heating film, hot lid aluminium sheet and flexible thermal Cover Gasket；It further include the controller on cabinet, lifting structure, the rotational structure being connect with lifting structure upper end, heat lid is connect with rotational structure, hot to cover equipped with multiple columns corresponding with each test tube jack in gene magnification module, is equipped with air bag in each column, air bag lower end is equipped with cover board, each air bag passes through gas-guide tube and connect with air accumulator, and pressure sensor is equipped in air accumulator, and air accumulator is equipped with air pump.The present invention has the characteristics that sealing effect is good, detection accuracy is high.
Description
Technical Field
The invention relates to the technical field of gene amplification equipment, in particular to a hot cover device of a gene amplification instrument with good sealing effect and high detection accuracy and a control method.
Background
The gene amplification instrument is an indispensable instrument in the fields of biological scientific research, gene replication and the like, and is also a necessary instrument for the research in the field of biological genetic engineering. When the gene expansion instrument is used, a gene solution to be amplified is added into a test tube, then related reagents are added into the test tube, the test tube is placed on a heating module in the gene expansion instrument to heat the test tube, and the gene to be tested in the test tube is rapidly amplified through temperature change, so that the gene amplification test requirement is met.
When doing the experiment of gene amplification instrument, must cover the upper cover of gene amplification instrument, through adjusting the hot lid of test tube mouth upper end, compress tightly and strictly sealed the test tube mouth, avoid the liquid in the test tube to lead to the test result to appear the deviation owing to being heated the evaporation.
Chinese patent grant publication no: CN201080478Y, Announcement date 2008, 07.02.9.A "hot cover pressing device for gene amplification instrument" comprises a pressing mechanism, a hot cover, a slide way and an adjusting knob, and is characterized in that a screw rod capable of lifting the pressing mechanism is arranged between the pressing mechanism and the adjusting knob, four connecting shafts between the pressing mechanism and the hot cover are respectively provided with homogeneous springs, the periphery of the pressing mechanism is provided with symmetrically distributed balance rollers, and the balance rollers are matched with the slide way to form sliding fit connection. The shortcomings are that the hot cover pressing device of the gene amplification instrument only has a simple lifting function, the pressure of the hot cover on the test tube cannot be accurately mastered in the lifting process, the pressing strength of the hot cover and the test tube can be adjusted only by the experience of an operator, and for the test tubes with different heights or different strengths, the operator needs to lift the pressing mechanism by a certain amount, so that the strength of crushing the test tube or pressing the test tube due to improper operation is easily caused by the experience of the operator.
Disclosure of Invention
The invention aims to overcome the defects of poor sealing effect and low detection precision in the prior art and provides a gene amplification instrument with good sealing effect and high detection precision and a control method.
In order to achieve the purpose, the invention adopts the following technical scheme:
a gene amplification instrument comprises a box body, a gene amplification module and a heating device, wherein the gene amplification module and the heating device are arranged in the box body; still including locating the controller on the box, elevation structure, the rotating-structure of being connected with the elevation structure upper end, hot lid is connected with rotating-structure, hot covering is equipped with the corresponding riser of each test tube jack in a plurality of and the gene amplification module, all be equipped with the gasbag in every riser, the gasbag lower extreme is equipped with the apron, each gasbag all passes through air duct and gas tank connection, be equipped with pressure sensor in the gas holder, be equipped with the air pump on the gas holder, all be equipped with the solenoid valve on every air duct, be equipped with temperature sensor in the gene amplification module, the controller respectively with temperature sensor, the heating film, the air pump, heating device, elevation structure, rotating-structure, sensor and each solenoid valve electricity are connected to calming anger.
The invention utilizes the air pressure of the air storage tank to uniformly control the pressure of the air bag on each cover plate, thereby keeping the pressure between the test tubes with different heights and the corresponding cover plates consistent, the lifting device and the rotating device are respectively used for lifting and rotating the hot cover, and each electromagnetic valve is used for controlling the air bag in the vertical groove with the test tube to be inflated.
Preferably, a cylindrical first electromagnet is arranged on the outer peripheral wall of each vertical cylinder, an annular second electromagnet is arranged on the side face of each cover plate, a third electromagnet and a plurality of elastic protrusions are arranged in the lower portion of each vertical cylinder, and a cushion pad is arranged on the end face, in contact with the test tube, of each elastic protrusion; each first electromagnet, each second electromagnet and each third electromagnet are electrically connected with the controller.
The first electromagnet and the second electromagnet are used for fixing the position of the cover plate, and the cover plate is prevented from being displaced due to unstable air pressure of the air bag. The third electromagnet is used for controlling the expansion and contraction of the elastic protrusion.
Preferably, the inner side wall of each vertical cylinder is provided with a plurality of vertical guide ribs, and the cover plate of each vertical cylinder is provided with a notch corresponding to each vertical guide rib. Each vertical guide rib provides a guide function for the movement of the cover plate.
Preferably, the lifting structure comprises a fixed plate arranged on the side wall of the box body, two vertical slide rails and a fixed frame; the fixed frame is connected with the two vertical sliding rails in a sliding manner, the lifting motor is connected with the fixed frame through a rotating shaft with external threads, and the lifting motor is electrically connected with the controller; the rotating structure comprises a shaft support frame arranged on the upper part of the fixed frame, a rotating shaft arranged on the shaft support frame, a rotating motor connected with the rotating shaft, and a rotating arm connected with the rotating shaft, wherein the rotating arm is connected with the hot cover, and the rotating motor is electrically connected with the controller.
Preferably, the hot cover aluminum plate comprises a rectangular cover plate and a rectangular coaming which is arranged on the edge of the rectangular cover plate and extends downwards and vertically, m through holes are arranged on the rectangular coaming, each through hole is communicated with the horn-shaped opening, and the horn-shaped openings are opened downwards; the flexible hot cover pad comprises a rectangular base plate and a bending edge which is arranged on the edge of the rectangular base plate and is bent upwards, and m bulges matched with the through holes are arranged on the rectangular base plate respectively.
A control method of a hot cover device of a gene amplification instrument comprises the following steps:
(6-1) putting a plurality of test tubes for experiments into each test tube jack respectively by a worker, controlling the third electromagnet to be electrified by the controller, and sucking each elastic protrusion back into the side wall of the vertical cylinder; the controller controls the rotating structure to drive the hot cover to rotate to a horizontal position, the controller controls the lifting structure to drive the hot cover to descend to a preset position, and the test tubes respectively extend into the vertical cylinder;
(6-2) detecting the pressure in the gas storage tank by the pressure sensor, controlling the air pump to work by the controller, gradually increasing the air pressure in the gas storage tank, and keeping the air pressure in the gas storage tank within a preset pressure range [ A1, A2] in the controller after T1 minutes;
for the vertical cylinder with the test tube, the controller controls the electromagnetic valve of the air guide tube connected with the air bag in the vertical cylinder to be opened, and the air bag drives each cover plate to move downwards;
(6-3)
(6-3-1) the controller controls the air pressure of the air storage tank to be maintained within a pressure range [ A1, A2 ];
(6-3-2) the controller controls the first electromagnet and the corresponding second electromagnet of the vertical cylinder with the test tube to be electrified, the first electromagnet and the second electromagnet attract each other under the action of magnetic force, and the cover plate is kept stable;
(6-4) the controller controls the heating film and the heating device to heat each test tube, the temperature sensor detects the temperature, a standard temperature range is arranged in the controller, and when the heating time in the standard temperature range reaches T, the controller controls the air pump to work to gradually reduce the air pressure in the air storage tank; the controller controls each first electromagnet and each second electromagnet to be powered off;
(6-5) the controller controls the heating film and the heating device to stop heating, and the controller controls each third electromagnet to be powered off, and each elastic protrusion extends out;
controller control elevation structure drives the heat lid and rises, and each test tube is risen by the protruding centre gripping of elasticity, and when the test tube left the box completely, the staff passed through controller control elevator motor stop work, and the staff takes out each test tube.
Preferably, the following steps are included between the step (6-3-1) and the step (6-3-2):
(7-1) selecting detection values of the front and rear pressure sensors in a time period with the length of L by the controller; the two time periods are respectively a time period A and a time period B, and if L is n multiplied by TT, the n detection values of the pressure sensors in the time period A and the time period B are obtained through control;
(7-2) setting each detection value Sc of the time period A to xiEach detection value Sc of the period B is yi，i＝1，2，...，n；
Using formulasCalculating the similarity of Sc corresponding to the two time periods;
(7-3) if siIf < 1, will be compared with siCorresponding to yiDeleting; wherein x is the average of all Sc in the time period A,is the average of all Sc over time period B;
(7-4) the controller utilizes the remaining y during the time period BiThe detection signal I (t) of the pressure sensor is formed;
(7-5) inputting I (t) into the coherent resonance model, and adjusting the mu value of the coherent resonance model to enable the coherent resonance model to resonate;
(7-6) outputting the cross correlation coefficient by the coherent resonance model, and if the cross correlation coefficient is in the interval [0.85, 1.1], switching to the step (6-3-2); otherwise, the step (6-3-1) is carried out.
The judgment of whether the cross correlation coefficient is in the set interval can fix the position of the cover plate in the interval of meeting the pressure requirement of the gas storage tank, thereby meeting the requirement of sealing property, ensuring that the test tube is not damaged, improving the detection accuracy, improving the success rate of the experiment and reducing the production cost.
Preferably, the coherent resonance model is
Wherein V (t) is the action potential of the model trigger unit, VTIs a model trigger action threshold potential, VRIs the recovery potential after the trigger unit action is completed, mu tau is the resting state parameter after the model trigger action, VR＜VTξ (t) Gaussian random excitation parameter, V (t) is the real-time potential of the coherent resonance model, μ is the adjustment coefficient of the coherent resonance model, τ is the rest constant of the coherent resonance model, and V (t)+) Is a coherent resonance model at t+Real-time potential of time, V2(t) is the square of V (t), μ2τ is μ2The product of τ.
Preferably, the edge of the lower surface of each cover plate is provided with a coaming perpendicular to the cover plate, an annular vertical groove is arranged in each coaming, and a sealing ring is arranged in each annular vertical groove.
The arrangement of the coaming, the annular vertical groove and the sealing ring ensures that the sealing effect of the cover plate is better.
Therefore, the invention has the following beneficial effects:
the pressure of each air bag and the corresponding cover plate is uniformly controlled by utilizing the air pressure of the air storage tank, so that the pressure between test tubes with different heights and the corresponding cover plates is kept consistent, and the requirement on the diversity of the test tube models used in experiments is met;
the sealing effect is good, the test tube is not easy to be damaged, the detection accuracy is improved, the experiment success rate is improved, and the production cost is reduced.
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a cross-sectional view of the thermal cover of the present invention;
FIG. 3 is a schematic exploded view of the thermal cover of the present invention;
FIG. 4 is a functional block diagram of the present invention;
fig. 5 is a flow chart of the present invention.
In the figure: the device comprises a box body 111, a hot cover pressing plate 1, a hot cover heat insulation pad 2, a heating film 3, a hot cover aluminum plate 4, a flexible hot cover pad 5, a controller 112, a lifting structure 113, a rotating structure 114, a vertical cylinder 115, an air bag 116, a cover plate 117, an air duct 119, an air storage tank 118, a pressure sensor 1181, an air pump 1182, an electromagnetic valve 1191, a first electromagnet 1110, a second electromagnet 1111, a third electromagnet 1112, a fixing plate 1131, a fixing frame 1133, a lifting motor 1134, a shaft support 1141, a rotating shaft 1142, a rotating motor 1143, a rotating arm 1144, a rectangular cover plate 42, a rectangular enclosing plate 43, a through hole 44, a horn-shaped opening 45, a rectangular base plate 51, a bent edge 52, a protrusion 53 and a nut 412.
Detailed Description
The invention is further described with reference to the following figures and detailed description.
As shown in fig. 1, the gene amplification instrument comprises a box 111, a gene amplification module and a heating device, which are arranged in the box, and as shown in fig. 3, the heat cover device comprises a heat cover pressing plate 1, a heat cover heat insulation pad 2, a heating film 3, a heat cover aluminum plate 4 and a flexible heat cover pad 5, which are sequentially arranged from top to bottom; still including locating controller 112 on the box, elevation structure 113, revolution mechanic 114 with the elevation structure upper end is connected, the hot lid is connected with revolution mechanic, as shown in fig. 2, be equipped with a plurality of and the corresponding vertical retort 115 of each test tube jack in the gene amplification module on the hot lid, all be equipped with gasbag 116 in every vertical retort, the gasbag lower extreme is equipped with apron 117, each gasbag all is connected with gas holder 118 through air duct 119, as shown in fig. 4, be equipped with pressure sensor 1181 in the gas holder, be equipped with air pump 1182 on the gas holder, all be equipped with solenoid valve 1191 on every air duct, be equipped with temperature sensor in the gene amplification module, the controller respectively with temperature sensor, the heating film, the air pump, heating device, elevation structure, revolution mechanic, the sensor and each solenoid valve electricity are connected.
A cylindrical first electromagnet 1110 is arranged on the outer peripheral wall of each vertical cylinder, an annular second electromagnet 1111 is arranged on the side surface of each cover plate, a third electromagnet 1112 and a plurality of elastic protrusions are arranged in the lower portion of each vertical cylinder, and a buffer pad is arranged on the end face of each elastic protrusion, which is in contact with the test tube; each first electromagnet and each second electromagnet are electrically connected with the controller.
The inner side wall of each vertical cylinder is provided with a plurality of vertical guide ribs, and the cover plate of each vertical cylinder is provided with a notch corresponding to each vertical guide rib.
As shown in fig. 1, the lifting structure includes a fixing plate 1131 disposed on the sidewall of the box body, a limit sensor 1132 disposed on the fixing plate, two vertical sliding rails, and a fixing frame 1133; the fixed frame is connected with the two vertical slide rails in a sliding manner, the lifting motor 1134 is connected with the fixed frame through a rotating shaft provided with external threads, and the limit sensor and the lifting motor are both electrically connected with the controller; the rotating structure comprises a shaft support 1141 arranged on the upper part of the fixed frame, a rotating shaft 1142 arranged on the shaft support, a rotating motor 1143 connected with the rotating shaft, and a rotating arm 1144 connected with the rotating shaft, wherein the rotating arm is connected with the hot cover, and the rotating motor is electrically connected with the controller.
As shown in fig. 3, the aluminum plate for the hot cover comprises a rectangular cover plate 42 and a rectangular enclosing plate 43 which is arranged on the edge of the rectangular cover plate and extends downwards vertically, wherein 14 through holes 44 are arranged on the rectangular enclosing plate, each through hole is communicated with a horn-shaped opening 45, and the horn-shaped openings are opened downwards; the flexible hot cover pad comprises a rectangular pad plate 51 and an upward bent edge 52 arranged on the edge of the rectangular pad plate, wherein the rectangular pad plate is provided with 14 protrusions 53 respectively matched with the through holes, and the flexible hot cover pad further comprises 4 screw caps 412.
The lower surface edge of every apron all is equipped with the bounding wall perpendicular and apron, is equipped with the annular in the bounding wall and erects the groove, the annular is erected the inslot and is equipped with the sealing washer that can contact with the test tube mouth.
As shown in FIG. 5, a method for controlling a hot lid device of a gene amplification apparatus includes the steps of:
step 100, installing a test tube and covering a hot cover
The staff puts 8 test tubes for experiments into each test tube jack respectively, the controller controls the third electromagnet to be electrified, and each elastic protrusion is sucked back into the side wall of the vertical cylinder; the controller controls the rotating structure to drive the hot cover to rotate to a horizontal position, the controller controls the lifting structure to drive the hot cover to descend to a preset position, and the test tubes respectively extend into the vertical cylinder;
step 200, inflating each air bag, and enabling each cover plate to be in contact with each test tube port
The pressure sensor detects the pressure in the gas storage tank, the controller controls the air pump to work, the air pressure in the gas storage tank is gradually increased, and after 5 minutes, the air pressure in the gas storage tank is always in a preset pressure range [ A1, A2] in the controller;
for the vertical cylinder with the test tube, the controller controls the electromagnetic valve of the air guide tube connected with the air bag in the vertical cylinder to be opened, and the air bag drives each cover plate to move downwards;
step 300, selecting a suitable interval to fix the cover plate
Step 310, the controller controls the air pressure of the air storage tank to be maintained within a pressure range [ A1, A2 ];
(7-1) selecting detection values of the front and rear pressure sensors in a time period with the length of L by the controller; the two preceding and succeeding time periods are respectively a time period A and a time period B, and if L is n multiplied by TT, n detection values of the pressure sensors in the time period A and the time period B are obtained through control, wherein n is 60, L is 1 minute, and LL is 1 second;
(7-2) setting each detection value Sc of the time period A to xiEach detection value Sc of the period B is yi，i＝1，2，...，n；
Using formulasCalculating the similarity of Sc corresponding to the two time periods;
(7-3) if siIf < 1, will be compared with siCorresponding to yiDeleting; wherein x is the average of all Sc in the time period A,is the average of all Sc over time period B;
(7-4) the controller utilizes the remaining y during the time period BiThe detection signal I (t) of the pressure sensor is formed;
(7-5) inputting I (t) into coherent resonance modelAdjusting the mu value of the coherent resonance model to enable the coherent resonance model to resonate;
wherein V (t) is the action potential of the model trigger unit, VTIs a model trigger action threshold potential, VRIs the recovery potential after the trigger unit action is completed, mu tau is the resting state parameter after the model trigger action, VR＜VTξ (t) Gaussian random excitation parameter, V (t) is the real-time potential of the coherent resonance model, μ is the adjustment coefficient of the coherent resonance model, τ is the rest constant of the coherent resonance model, and V (t)+) Is a coherent resonance model at t+Real-time potential of time, V2(t) is the square of V (t), μ2τ is μ2The product of τ;
(7-6) outputting the cross correlation coefficient by the coherent resonance model, and if the cross correlation coefficient is in the interval [0.85, 1.1], turning to the step 320; otherwise, go to step 310.
320, controlling the first electromagnet and the corresponding second electromagnet of the vertical groove with the test tube to be electrified by the controller, wherein the first electromagnet and the second electromagnet attract each other under the action of magnetic force, and the cover plate is kept stable;
step 400, heating the test tube
The controller controls the heating film and the heating device to heat each test tube, the temperature sensor detects the temperature, a standard temperature range is arranged in the controller, and when the heating time in the standard temperature range reaches 2 hours, the controller controls the air pump to work to gradually reduce the air pressure in the air storage tank; the controller controls each first electromagnet and each second electromagnet to be powered off;
step 500, taking out the test tube
The controller controls the heating film and the heating device to stop heating, and controls each third electromagnet to be powered off and each elastic protrusion to extend out;
controller control elevation structure drives the heat lid and rises, and each test tube is risen by the protruding centre gripping of elasticity, and when the test tube left the box completely, the staff passed through controller control elevator motor stop work, and the staff takes out each test tube.
It should be understood that this example is for illustrative purposes only and is not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Claims (9)
1. A gene amplification instrument with a heat cover device comprises a box body (111), a gene amplification module and a heating device, wherein the gene amplification module and the heating device are arranged in the box body, and the gene amplification instrument is characterized in that the heat cover device comprises a heat cover pressing plate (1), a heat cover heat insulation pad (2), a heating film (3), a heat cover aluminum plate (4) and a flexible heat cover pad (5) which are sequentially arranged from top to bottom; still including locating controller (112) on the box, elevation structure (113), rotating-structure (114) of being connected with the elevation structure upper end, the hot lid is connected with rotating-structure, be equipped with corresponding riser (115) of each test tube jack in a plurality of and the gene amplification module on the hot lid, all be equipped with gasbag (116) in every riser, the gasbag lower extreme is equipped with apron (117), each gasbag all is connected with gas holder (118) through air duct (119), be equipped with pressure sensor (1181) in the gas holder, be equipped with air pump (1182) on the gas holder, all be equipped with solenoid valve (1191) on every air duct, be equipped with temperature sensor in the gene amplification module, the controller respectively with temperature sensor, the heating film, the air pump, heating device, elevation structure, rotating-structure, compressed air sensor and each solenoid valve electricity are connected.
2. The gene amplification apparatus with a heat cover device according to claim 1, wherein the outer peripheral wall of each vertical cylinder is provided with a first cylindrical electromagnet (1110), the side surface of each cover plate is provided with a second annular electromagnet (1111), the lower part of each vertical cylinder is provided with a third electromagnet (1112) and a plurality of elastic protrusions, and the end surfaces of the elastic protrusions contacting with the test tubes are provided with buffer pads; each first electromagnet, each second electromagnet and each third electromagnet are electrically connected with the controller.
3. The gene amplification instrument with a heat cover device of claim 1, wherein the inner sidewall of each vertical tube is provided with a plurality of vertical guide ribs, and the cover plate of each vertical tube is provided with notches corresponding to the vertical guide ribs.
4. The gene amplification instrument with the heat cover device according to claim 1, wherein the lifting structure comprises a fixing plate (1131), two vertical slide rails and a fixing frame (1133) which are arranged on the side wall of the box body; the fixed frame is connected with the two vertical slide rails in a sliding manner, the lifting motor (1134) is connected with the fixed frame through a rotating shaft provided with external threads, and the lifting motors are electrically connected with the controller; the rotating structure comprises a shaft support frame (1141) arranged on the upper part of the fixed frame, a rotating shaft (1142) arranged on the shaft support frame, a rotating motor (1143) connected with the rotating shaft, and a rotating arm (1144) connected with the rotating shaft, wherein the rotating arm is connected with the hot cover, and the rotating motor is electrically connected with the controller.
5. The gene amplification apparatus with a heat cover device according to claim 1, 2, 3 or 4, wherein the heat cover aluminum plate comprises a rectangular cover plate (42) and a rectangular surrounding plate (43) which is arranged on the edge of the rectangular cover plate and extends vertically downwards, the rectangular surrounding plate is provided with m through holes (44), each through hole is communicated with a flared opening (45), and the flared opening is opened downwards; the flexible hot cover pad comprises a rectangular base plate (51) and a bent edge (52) which is arranged on the edge of the rectangular base plate and is bent upwards, wherein m bulges (53) matched with the through holes are arranged on the rectangular base plate respectively.
6. A method for controlling the gene amplification apparatus having the hot lid device according to claim 2, comprising the steps of:
(6-1) putting a plurality of test tubes for experiments into each test tube jack respectively by a worker, controlling the third electromagnet to be electrified by the controller, and sucking each elastic protrusion back into the side wall of the vertical cylinder; the controller controls the rotating structure to drive the hot cover to rotate to a horizontal position, the controller controls the lifting structure to drive the hot cover to descend to a preset position, and the test tubes respectively extend into the vertical cylinder;
(6-2) detecting the pressure in the gas storage tank by the pressure sensor, controlling the air pump to work by the controller, gradually increasing the air pressure in the gas storage tank, and keeping the air pressure in the gas storage tank within a preset pressure range [ A1, A2] in the controller after T1 minutes;
for the vertical cylinder with the test tube, the controller controls the electromagnetic valve of the air guide tube connected with the air bag in the vertical cylinder to be opened, and the air bag drives each cover plate to move downwards;
(6-3)
(6-3-1) the controller controls the air pressure of the air storage tank to be maintained within a pressure range [ A1, A2 ];
(6-3-2) the controller controls the first electromagnet and the corresponding second electromagnet of the vertical cylinder with the test tube to be electrified, the first electromagnet and the second electromagnet attract each other under the action of magnetic force, and the cover plate is kept stable;
(6-4) the controller controls the heating film and the heating device to heat each test tube, the temperature sensor detects the temperature, a standard temperature range is arranged in the controller, and when the heating time in the standard temperature range reaches T, the controller controls the air pump to work to gradually reduce the air pressure in the air storage tank; the controller controls each first electromagnet and each second electromagnet to be powered off;
(6-5) the controller controls the heating film and the heating device to stop heating, and the controller controls each third electromagnet to be powered off, and each elastic protrusion extends out;
controller control elevation structure drives the heat lid and rises, and each test tube is risen by the protruding centre gripping of elasticity, and when the test tube left the box completely, the staff passed through controller control elevator motor stop work, and the staff takes out each test tube.
7. The method for controlling a gene amplification apparatus having a hot lid unit according to claim 6, wherein the following steps are included between the step (6-3-1) and the step (6-3-2):
(7-1) selecting detection values of the front and rear pressure sensors in a time period with the length of L by the controller; the two time periods are respectively a time period A and a time period B, and if L is n multiplied by TT, the n detection values of the pressure sensors in the time period A and the time period B are obtained through control;
(7-2) setting each detection value Sc of the time period A to xiEach detection value Sc of the period B is yi，i＝1，2，...，n；
Using formulasCalculating the similarity of Sc corresponding to the two time periods;
(7-3) if siIf < 1, will be compared with siCorresponding to yiDeleting; wherein,is the average of all Sc over the time period a,is the average of all Sc over time period B;
(7-4) the controller utilizes the remaining y during the time period BiThe detection signal I (t) of the pressure sensor is formed;
(7-5) inputting I (t) into the coherent resonance model, and adjusting the mu value of the coherent resonance model to enable the coherent resonance model to resonate;
(7-6) outputting the cross correlation coefficient by the coherent resonance model, and if the cross correlation coefficient is in the interval [0.85, 1.1], switching to the step (6-3-2); otherwise, the step (6-3-1) is carried out.
8. The method as set forth in claim 7, wherein the coherent resonance model is a model
Wherein V (t) is the action potential of the model trigger unit, VTIs a model trigger action threshold potential, VRIs the recovery potential after the trigger unit action is completed, mu tau is the resting state parameter after the model trigger action, VR＜VTξ (t) Gaussian random excitation parameter, V (t) is the real-time potential of the coherent resonance model, μ is the adjustment coefficient of the coherent resonance model, τ is the rest constant of the coherent resonance model, and V (t)+) Is a coherent resonance model at t+Real-time potential of time, V2(t) is the square of V (t), μ2τ is μ2The product of τ.
9. The method for controlling a gene amplification apparatus having a heat cap device according to claim 7 or 8, wherein a surrounding plate is provided on the lower surface edge of each cover plate to be perpendicular to the cover plate, an annular vertical groove is provided in the surrounding plate, and a sealing ring capable of contacting the test tube port is provided in the annular vertical groove.
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