CN106978365A - The preparation method and a kind of fermentation preparation of a kind of selenium-rich cheese production zymophyte powder - Google Patents
The preparation method and a kind of fermentation preparation of a kind of selenium-rich cheese production zymophyte powder Download PDFInfo
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Abstract
The present invention relates to selenium-rich cheese production fermentation preparation, the biological function of lactic acid bacteria and selenium is had concurrently, its simple production process, the selenium-rich cheese production fermentation preparation can be used to efficiently prepare selenium-rich cheese and relevant healthcare food, have broad application prospects.Wherein, the preparation method of zymophyte powder is：A., lactic acid bacteria is routinely cultivated to amplification；B. sodium selenite solution is added into zymotic fluid, the Se content for making zymotic fluid is 30~120mg/L, continues incubated 12~36 hours, obtains bacterium solution；C. after bacterium solution is centrifuged, washing precipitate, then centrifuge, go to produce product after supernatant, freeze-dried machine are lyophilized.
Description
Technical field
The present invention relates to cheese preparation field, more particularly to a kind of preparation method and one of selenium-rich cheese production zymophyte powder
Plant fermentation preparation.
Background technology
Selenium is the essential trace elements of the human body, at present, due to reasons such as technique and costs, and the selenium in health care of food product is main
Or based on inorganic selenium, such as sodium selenate and sodium selenite, but research has shown that human body is poor to the assimilation effect of inorganic selenides,
Utilization rate is low, can produce the different toxic side effect of degree to human body again；And organic selenides utilization rate is high, make without any poison pair
With.
The two kinds of means that organise of selenium：Artificial synthesized and bioconversion.Artificial synthesized product is a lot, but price is high
It is expensive, and the product of conversion is single, it is difficult to applied in actual production.The bio-organic of selenium is mainly by animal, plant, micro-
Bioconversion inorganic selenium produces Organic Selenium, such as：Se-enriched egg, Agaricus campestris rich in selenium, Se-enriched yeast etc., selenium therein is mainly with seleno
The forms such as cysteine, selenocystine, selenomethionine, methylselenocysteine are present.But animals and plants conversion production has
The machine selenium cycle is long, and cost is high.
The feature selenium-enriched food base-material obtained at present by yeast selenium-rich has started to industrialized production and application.But selenium-rich
Yeast selenium content is usually 0.3mg/g, up to 1mg/g, still not ideal enough to the trans-utilization of inorganic selenium.Lactic acid bacteria is main
One of industrial food microorganism, is mostly probiotics, with antibacterial, reduction cholesterol, maintains micro-ecological environment, antitumor, increasing
The biological functions such as strong immunity.
Cheese be a kind of curdled milk bacterium (being mostly lactic acid bacteria) fermentation natural dairy products, be western countries' staple food it
One of most important diatery supplement of one, especially infant.Because cheese has high nutritive value, increasingly consumed by China
Person welcomes.Lactic acid bacteria does not have the biological function of selenium in itself, but research finds that inorganic selenium can be converted into by some lactic acid bacterias
Organic Selenium, and transformation efficiency is high.
The content of the invention
The present invention is exactly in view of the deficienciess of the prior art, there is provided a kind of preparation of selenium-rich cheese production zymophyte powder
Method and a kind of fermentation preparation.
To solve the above problems, the technical solution used in the present invention is as follows：
A kind of preparation method of selenium-rich cheese production zymophyte powder, comprises the following steps：
S1：By lactobacillus inoculum in MRS culture mediums, the lactic acid bacteria includes Lactobacillus plantarum or lactobacillus paracasei pair
Cheese subspecies or lactobacillus delbruockii subspecies bulgaricus or streptococcus thermophilus, activation amplification cultivation 12-24 hours；
S2：MRS culture mediums separately are manufactured separately；
S3：Sodium selenite is added in MRS culture mediums in S2 so that selenium concentration is 30-120mg/L in culture medium；Go out
After bacterium, the activated spawn in access S1 is 4-5%, incubated 20-36 by the inoculum concentration of quality volume percentage activation strain
Hour, obtain bacterium solution；
S4：Bacterium solution in S3 is centrifuged, washing precipitate at least 3 times after centrifugation, then centrifuge, remove supernatant, freezing is dry
It is dry, obtain lyophilized selenium-rich lactobacillus powder.
Preferably, in S3, the mass fraction of sodium selenite solution is to decoction after 5%-8%, 121 DEG C of sterilizing 30min
It is middle to be inoculated with, it is incubated after inoculation until selenium-rich lactobacillus cell degree ＞ 109CFU/mL。
Preferably, in S4, from sterile saline washing precipitate.
Preferably, the MRS culture mediums are made in the following ways：By addition peptone 10.0g in 1000ml distilled water,
Beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, sodium acetate 5.0g, lemon acid diamine 2.6g, Tween 80 1.0g,
K2HPO42.0g, MgSO4·7H2O 0.58g, MnSO4·H2O 0.25g, mixed dissolution simultaneously adjusts pH to 6.0-6.4,120-125
DEG C sterilizing 15-20min.
The present invention also proposes a kind of fermentation preparation, the fermentation preparation include the secondary cheese subspecies bacterium powder of lactobacillus paracasei,
Lactobacillus delbrueckii subspecies bulgaricus powder, streptococcus thermophilus bacterium powder, by weight, 2-3 parts of Lactobacillus plantarum bacterium powder, pair are dry
1-2 parts of Lactobacillus paracasei pair cheese subspecies bacterium powder, 2-3 parts of lactobacillus delbrueckii subspecies bulgaricus powder, streptococcus thermophilus bacterium powder 3-4
Part.
Preferably, the fermentation preparation is by Lactobacillus plantarum, the secondary cheese subspecies bacterium powder of lactobacillus paracasei, Lactobacillus delbrueckii
Subspecies bulgaricus bacterium powder, streptococcus thermophilus bacterium powder are fully mixed evenly to prepare by 3: 2: 2: 3.
Compared with prior art, implementation result of the invention is as follows by the present invention：
The fermentation preparation that the present invention is provided, efficiently can be converted into Organic Selenium, so as to prepare excellent in flavor by inorganic selenium
Selenium-rich cheese, its simple production process, easy to operate, high conversion rate are in yeast selenium-rich, and Market implementation is workable, has
Wide application prospect
Brief description of the drawings
Fig. 1 is the schematic flow sheet of the preparation method of selenium-rich cheese production zymophyte powder proposed by the present invention.
Embodiment
Illustrate present disclosure below in conjunction with specific embodiments.
As shown in figure 1, Fig. 1 is the flow signal of the preparation method of selenium-rich cheese production zymophyte powder proposed by the present invention
Figure.
A kind of preparation method of selenium-rich cheese production zymophyte powder, comprises the following steps：
S1：By lactobacillus inoculum in the medium, the lactic acid bacteria includes Lactobacillus plantarum or lactobacillus paracasei pair is dry
Junket subspecies or lactobacillus delbruockii subspecies bulgaricus or streptococcus thermophilus, activation amplification cultivation 12-24 hours；
S2：MRS culture mediums separately are manufactured separately；
S3：Sodium selenite is added in MRS culture mediums in S2 so that selenium concentration is 30-120mg/L in culture medium；Go out
After bacterium, the activated spawn in access S1 is 4-5%, incubated 20-36 by the inoculum concentration of quality volume percentage activation strain
Hour, obtain bacterium solution；
S4：Bacterium solution in S3 is centrifuged, washing precipitate at least 3 times after centrifugation, then centrifuge, remove supernatant, freezing is dry
It is dry, obtain lyophilized selenium-rich lactobacillus powder.
Preferably, in S3, the mass fraction of sodium selenite solution is to decoction after 5%-8%, 121 DEG C of sterilizing 30min
It is middle to be inoculated with, it is incubated after inoculation until selenium-rich lactobacillus cell degree ＞ 109CFU/mL。
Preferably, in S4, from sterile saline washing precipitate.
Preferably, the MRS culture mediums are made in the following ways：By addition peptone 10.0g in 1000ml distilled water,
Beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, sodium acetate 5.0g, lemon acid diamine 2.6g, Tween 80 1.0g,
K2HPO42.0g, MgSO4·7H2O 0.58g, MnSO4·H2O 0.25g, mixed dissolution simultaneously adjusts pH to 6.0-6.4,120-125
DEG C sterilizing 15-20min.
Need to measure Se content in process of production, this programme measuring method is as follows：
(1) standard selenium working solution prepares
Precision weighs the pure sodium selenite of analysis, plus the Standard Reserving Solution of every milliliter of selenium containing 1.0mg is made in salpeter solution；It is accurate
Standard Reserving Solution 1mL is measured, 100mL, as standard selenium working solution are settled to salpeter solution.
(2) treatments of the sample
Precision weighs 0.1g selenium-enriched plant Lemonal dry powder samples in small beaker, plus the wet sample of a small amount of water, Ran Houzai
Digestive juice (hydrogen peroxide: perchloric acid: the concentrated sulfuric acid 23: 3: 1) is added, is shaken up.Put after being digested to terminal on electric furnace in fume hood, plus
5%EDTA-2Na solution 2mL, shake up, and adjust PH to neutrality in cold bath with 40% sodium hydroxide, are settled to 100mL, are done with method
Compare blank assay.
(3) sample measurement and standard curve are formulated
Precision measures 5mL digestive juices and 7 separatory funnels is taken in separatory funnel, separately, is separately added into and digestive juice equivalent
Blank solution and standard selenium working solution 0.0,2.0,4.0,6.0,8.0,10.0mL, 5% sodium hydroxide solution adjust PH to neutrality, plus
2mol/L formic acid solution 3mL, then add water to 35mL, shake up.
0.5%3,3 '-diaminobenzidine solution 5.0mL are added in each separatory funnel, rearmounted dark place reaction is shaken up
30min.PH6.5-7.0 is adjusted with 5% sodium hydroxide solution, precision adds toluene 10mL, shake extraction 1min, stratification takes
Toluene layer filtrate is in 1mL cuvettes, densitometric (OD) value at wavelength 420nm.Drawn with the OD values of standard selenium working solution
Standard curve, finds Se content corresponding with treatments of the sample liquid on curve.Standard selenium is in the range of 0.02-0.1mg/mL in good
Good linear relationship, regression equation is y=0.045+0.114x, degree of fitting (R2) more than 0.99.After the OD values for determining digestive juice
It is that can obtain the Se content of the selenium-rich lactobacillus preparation to substitute into regression equation.
Embodiment one
Lactobacillus plantarum is inoculated with the usual way in the medium, activation amplification cultivation 12~24 hours.Specifically, institute
Lactobacillus plantarum is stated for Lactobacillus plantarum CICC 21784 or Lactobacillus plantarum
CICC 20261 or Lactobacillus plantarum CICC 20765.
By addition peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g in 1000ml distilled water,
Sodium acetate 5.0g, lemon acid diamine 2.6g, Tween 80 1.0g, K2HPO42.0g, MgSO4·7H2O 0.58g, MnSO4·H2O
0.25g, mixed dissolution simultaneously adjusts pH to 6.0-6.4,120-125 DEG C of sterilizing 15-20min, obtains culture medium.
The sodium selenite that mass fraction is 8% is added in above-mentioned culture medium so that selenium concentration is 40- in culture medium
41mg/L；After sterilizing, above-mentioned activated spawn is accessed, is 4-5%, constant temperature training by the inoculum concentration of quality volume percentage activation strain
Support 20-36 hours, obtain bacterium solution.Preferably, it is 5% by the inoculum concentration of the activated spawn of quality percent by volume, it is incubated
24 hours.
Above-mentioned bacterium solution is centrifuged, washing precipitate at least 3 times after centrifugation, wherein, the rotating speed of rotating shaft is during centrifugation
2000~2100r/min, 10~15min, then centrifuge, remove supernatant, it is freeze-dried, obtains the Lactobacillus plantarum system rich in Organic Selenium
Agent, organic selenium content is up to 11.26-17.72mg/g.
Embodiment two
By the secondary cheese subspecies inoculation of lactobacillus paracasei in the medium, activation amplification cultivation 12~24 hours.Specifically,
The secondary cheese subspecies of the lactobacillus paracasei are Lactobacillusparacasei subsp.paracasei CICC 20296
Or Lactobacillus paracasei subsp.paracasei CICC22812.
By addition peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g in 1000ml distilled water,
Sodium acetate 5.0g, lemon acid diamine 2.6g, Tween 80 1.0g, K2HPO42.0g, MgSO4·7H2O 0.58g, MnSO4·H2O
0.25g, mixed dissolution simultaneously adjusts pH to 6.0-6.4,120-125 DEG C of sterilizing 15-20min, obtains culture medium.
The sodium selenite that mass fraction is 8% is added in above-mentioned culture medium so that selenium concentration is 115- in culture medium
125mg/L；After sterilizing, above-mentioned activated spawn is accessed, is 4-5%, constant temperature by the inoculum concentration of quality volume percentage activation strain
Culture 20-36 hours, obtains bacterium solution.Preferably, it is 5% by the inoculum concentration of the activated spawn of quality percent by volume, constant temperature training
Support 24 hours.
Above-mentioned bacterium solution is centrifuged, washing precipitate at least 3 times after centrifugation, wherein, the rotating speed of rotating shaft is during centrifugation
2000~2100r/min, 10~15min, then centrifuge, remove supernatant, it is freeze-dried, obtains the Lactobacillus plantarum system rich in Organic Selenium
Agent, organic selenium content is up to 8-12mg/g.
Embodiment three
By lactobacillus delbruockii subspecies bulgaricus inoculation in the medium, activation amplification cultivation 12~24 hours.Specifically,
The lactobacillus delbruockii subspecies bulgaricus are Lactobacillus delbrueckii subsp.bulgaricus CICC
20253, Lactobacillus delbrueckii subsp.bulgaricus CICC20271.
By addition peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g in 1000ml distilled water,
Sodium acetate 5.0g, lemon acid diamine 2.6g, Tween 80 1.0g, K2HPO42.0g, MgSO4·7H2O 0.58g, MnSO4·H2O
0.25g, mixed dissolution simultaneously adjusts pH to 6.0-6.4,120-125 DEG C of sterilizing 15-20min, obtains culture medium.
The sodium selenite that mass fraction is 8% is added in above-mentioned culture medium so that selenium concentration is 115- in culture medium
125mg/L；After sterilizing, above-mentioned activated spawn is accessed, is 4-5%, constant temperature by the inoculum concentration of quality volume percentage activation strain
Culture 20-36 hours, obtains bacterium solution.Preferably, it is 5% by the inoculum concentration of the activated spawn of quality percent by volume, constant temperature training
Support 24 hours.
Above-mentioned bacterium solution is centrifuged, washing precipitate at least 3 times after centrifugation, wherein, the rotating speed of rotating shaft is during centrifugation
2000~2100r/min, 10~15min, then centrifuge, remove supernatant, it is freeze-dried, obtains the Lactobacillus plantarum system rich in Organic Selenium
Agent, organic selenium content is up to 8-12mg/g.
Example IV
By streptococcus thermophilus inoculation in the medium, activation amplification cultivation 12~24 hours.Specifically, the thermophilus
Bacterium inoculation is Streptococcus thermophilus CICC 20370.
By addition peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g in 1000ml distilled water,
Sodium acetate 5.0g, lemon acid diamine 2.6g, Tween 80 1.0g, K2HPO42.0g, MgSO4·7H2O 0.58g, MnSO4·H2O
0.25g, mixed dissolution simultaneously adjusts pH to 6.0-6.4,120-125 DEG C of sterilizing 15-20min, obtains culture medium.
The sodium selenite that mass fraction is 8% is added in above-mentioned culture medium so that selenium concentration is 115- in culture medium
125mg/L；After sterilizing, above-mentioned activated spawn is accessed, is 4-5%, constant temperature by the inoculum concentration of quality volume percentage activation strain
Culture 20-36 hours, obtains bacterium solution.Preferably, it is 5% by the inoculum concentration of the activated spawn of quality percent by volume, constant temperature training
Support 24 hours.
Above-mentioned bacterium solution is centrifuged, washing precipitate at least 3 times after centrifugation, wherein, the rotating speed of rotating shaft is during centrifugation
2000~2100r/min, 10~15min, then centrifuge, remove supernatant, it is freeze-dried, obtains the Lactobacillus plantarum system rich in Organic Selenium
Agent, organic content of iodine is up to 10-16mg/g.
The present invention also proposes a kind of fermentation preparation, the fermentation preparation include the secondary cheese subspecies bacterium powder of lactobacillus paracasei,
Lactobacillus delbrueckii subspecies bulgaricus powder, streptococcus thermophilus bacterium powder, by weight, 2-3 parts of Lactobacillus plantarum bacterium powder, pair are dry
1-2 parts of Lactobacillus paracasei pair cheese subspecies bacterium powder, 2-3 parts of lactobacillus delbrueckii subspecies bulgaricus powder, streptococcus thermophilus bacterium powder 3-4
Part.
The secondary cheese subspecies bacterium powder of the lactobacillus paracasei, lactobacillus delbrueckii subspecies bulgaricus powder, streptococcus thermophilus
It is prepared by method of the bacterium powder respectively as described in embodiment one, two, three, four.
Preferably, the fermentation preparation is by Lactobacillus plantarum, the secondary cheese subspecies bacterium powder of lactobacillus paracasei, Lactobacillus delbrueckii
Subspecies bulgaricus bacterium powder, streptococcus thermophilus bacterium powder are fully mixed evenly to prepare by 3: 2: 2: 3.
Mentioned microorganism strain is provided by Chinese industrial Culture Collection.
The selenium-rich cheese production fermentation preparation that the present invention is provided, efficiently can be converted into Organic Selenium by inorganic selenium, so that
The cheese of excellent in flavor is prepared, its simple production process, easy to operate, high conversion rate are in yeast selenium-rich, and Market implementation is operable
Property it is strong, have broad application prospects.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (6)
1. a kind of preparation method of selenium-rich cheese production zymophyte powder, it is characterised in that comprise the following steps：
S1：By lactobacillus inoculum in the medium, the lactic acid bacteria includes Lactobacillus plantarum or the secondary cheese of lactobacillus paracasei is sub-
Plant or lactobacillus delbruockii subspecies bulgaricus or streptococcus thermophilus, activation amplification cultivation 12-24 hours；
S2：MRS culture mediums separately are manufactured separately；
S3：Sodium selenite is added in MRS culture mediums in S2 so that selenium concentration is 30-120mg/L in culture medium；After sterilizing,
The activated spawn accessed in S1, is 4-5% by the inoculum concentration of quality volume percentage activation strain, incubated 20-36 hours,
Obtain bacterium solution；
S4：Bacterium solution in S3 is centrifuged, washing precipitate at least 3 times after centrifugation, then centrifuge, remove supernatant, be freeze-dried, obtain
To lyophilized selenium-rich lactobacillus powder.
2. the preparation method of selenium-rich cheese production zymophyte powder according to claim 1, it is characterised in that in S3, sub-
The mass fraction of selenic acid sodium solution be 5%-8%, 121 DEG C sterilizing 30min after be inoculated with into decoction, it is incubated after inoculation
Until selenium-rich lactobacillus cell degree ＞ 109CFU/mL。
3. the preparation method of selenium-rich cheese production zymophyte powder according to claim 1 or 2, it is characterised in that in S4,
From sterile saline washing precipitate.
4. the preparation method of the selenium-rich cheese production zymophyte powder according to claim 1 or 2 or 3 any one, its feature exists
In the MRS culture mediums are made in the following ways：By addition peptone 10.0g, beef extract 10.0g in 1000ml distilled water,
Yeast extract 5.0g, glucose 20.0g, sodium acetate 5.0g, lemon acid diamine 2.6g, Tween 80 1.0g, K2HPO42.0g,
MgSO4·7H2O 0.58g, MnSO4·H2O 0.25g, mixed dissolution simultaneously adjusts pH to 6.0-6.4,120-125 DEG C of sterilizing 15-
20min。
5. a kind of fermentation using as prepared by the preparation method of any one of claim 1-4 selenium-rich cheese production zymophyte powder
Preparation, it is characterised in that the selenium-rich cheese production fermentation preparation includes the secondary cheese subspecies bacterium powder of lactobacillus paracasei, De Shi breasts
Bacillus subspecies bulgaricus bacterium powder, streptococcus thermophilus bacterium powder, by weight, 2-3 parts of Lactobacillus plantarum bacterium powder, the newborn bar of secondary cheese
1-2 parts of bacterium pair cheese subspecies bacterium powder, 2-3 parts of lactobacillus delbrueckii subspecies bulgaricus powder, 3-4 parts of streptococcus thermophilus bacterium powder.
6. fermentation preparation according to claim 5, it is characterised in that the fermentation preparation is by Lactobacillus plantarum, secondary cheese
Lactobacillus pair cheese subspecies bacterium powder, lactobacillus delbrueckii subspecies bulgaricus powder, streptococcus thermophilus bacterium powder are fully mixed by 3: 2: 2: 3
It is made after even.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699528A (en) * | 2017-11-22 | 2018-02-16 | 恩施清江生物工程有限公司 | Lactobacillus paracasei and its strain domestication method and cultural method |
| CN108048363A (en) * | 2018-01-16 | 2018-05-18 | 华侨大学 | A kind of method of selenium-rich lactobacillus reuteri high density fermentation |
| CN111004753A (en) * | 2019-12-30 | 2020-04-14 | 苏州硒泰克生物科技有限公司 | Lactobacillus paracasei capable of highly producing selenocysteine and application thereof |
| CN111187758A (en) * | 2020-02-07 | 2020-05-22 | 陕西科技大学 | Method for improving activity of thioredoxin reductase of lactic acid bacteria |
| CN111304115A (en) * | 2019-12-30 | 2020-06-19 | 江南大学 | Lactobacillus casei capable of highly producing 3 forms of organic selenium and application thereof |
| CN114836342A (en) * | 2022-04-19 | 2022-08-02 | 焦作市佰役安生物工程有限公司 | Lactobacillus GS-3 and application thereof in selenium enrichment |
| CN115039813A (en) * | 2022-07-20 | 2022-09-13 | 陕西科技大学 | Selenium-rich metazoan goat milk bar and preparation method thereof |
| CN116569989A (en) * | 2023-04-18 | 2023-08-11 | 华南理工大学 | Biological organic selenium feed additive and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699528A (en) * | 2017-11-22 | 2018-02-16 | 恩施清江生物工程有限公司 | Lactobacillus paracasei and its strain domestication method and cultural method |
| CN108048363A (en) * | 2018-01-16 | 2018-05-18 | 华侨大学 | A kind of method of selenium-rich lactobacillus reuteri high density fermentation |
| CN111004753A (en) * | 2019-12-30 | 2020-04-14 | 苏州硒泰克生物科技有限公司 | Lactobacillus paracasei capable of highly producing selenocysteine and application thereof |
| CN111304115A (en) * | 2019-12-30 | 2020-06-19 | 江南大学 | Lactobacillus casei capable of highly producing 3 forms of organic selenium and application thereof |
| CN111004753B (en) * | 2019-12-30 | 2022-09-06 | 苏州硒泰克生物科技有限公司 | Lactobacillus paracasei capable of highly producing selenocysteine and application thereof |
| CN111187758A (en) * | 2020-02-07 | 2020-05-22 | 陕西科技大学 | Method for improving activity of thioredoxin reductase of lactic acid bacteria |
| CN114836342A (en) * | 2022-04-19 | 2022-08-02 | 焦作市佰役安生物工程有限公司 | Lactobacillus GS-3 and application thereof in selenium enrichment |
| CN114836342B (en) * | 2022-04-19 | 2023-08-18 | 焦作市佰役安生物工程有限公司 | Lactic acid bacteria GS-3 and application thereof in selenium enrichment |
| CN115039813A (en) * | 2022-07-20 | 2022-09-13 | 陕西科技大学 | Selenium-rich metazoan goat milk bar and preparation method thereof |
| CN116569989A (en) * | 2023-04-18 | 2023-08-11 | 华南理工大学 | Biological organic selenium feed additive and preparation method and application thereof |
| CN116569989B (en) * | 2023-04-18 | 2024-04-23 | 华南理工大学 | Biological organic selenium feed additive and preparation method and application thereof |
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