CN106974063A - It is a kind of that the method that bacillus coagulans produces High efficiency protein feed is cooperateed with feeding enzyme - Google Patents
It is a kind of that the method that bacillus coagulans produces High efficiency protein feed is cooperateed with feeding enzyme Download PDFInfo
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- CN106974063A CN106974063A CN201710258194.9A CN201710258194A CN106974063A CN 106974063 A CN106974063 A CN 106974063A CN 201710258194 A CN201710258194 A CN 201710258194A CN 106974063 A CN106974063 A CN 106974063A
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- bacillus coagulans
- high efficiency
- dregs
- protein feed
- fermentation
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- 241000193749 Bacillus coagulans Species 0.000 title claims abstract description 60
- 229940054340 bacillus coagulans Drugs 0.000 title claims abstract description 60
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 58
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 20
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 20
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 54
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 54
- 238000000855 fermentation Methods 0.000 claims abstract description 48
- 230000004151 fermentation Effects 0.000 claims abstract description 46
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 36
- 241000894006 Bacteria Species 0.000 claims abstract description 34
- 239000000463 material Substances 0.000 claims abstract description 27
- 239000004310 lactic acid Substances 0.000 claims abstract description 18
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 18
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- 230000000694 effects Effects 0.000 claims abstract description 10
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- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims abstract description 5
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of method for cooperateing with bacillus coagulans to produce High efficiency protein feed with feeding enzyme, belongs to biotechnology fermentation field.The present invention is using bacterium enzyme collaboration processing dregs of beans, and without carrying out sterilization treatment, whole process amphimicrobian and anaerobic fermentation to dregs of beans, energy consumption is small, and dry loss is low.The technological process of the present invention is easy to operate, has both been adapted to plant's directly production and has fed, also has been adapted for feed factory large-scale production, and cost of material is low, is adapted to industrialized production.The fermented bean dregs of the present invention have stronger sour fragrance, and palatability is strong.PH is in 3.2~4.5, organic acid content more than 2.8%；Acid-soluble protein improves more than 6.5 times before relatively fermenting, and soluble protein relative molecular weight accounts for more than 60% within 500；Urease activity is less than 0.073U/g, and cottonseed sugared content is less than 0.42%, and stachyose is less than 0.035%；Lactic acid bacteria gemma number is condensed up to 2.0 × 109More than cfu/g.
Description
Technical field
The invention belongs to biotechnology fermentation field, and in particular to a kind of high with the collaboration bacillus coagulans production of feeding enzyme
The method for imitating protein feed.
Background technology
Animal to the absorption of high molecular weight protein apparently without the good absorbing to micromolecule polypeptide and amino acid, while vegetalitas
Protein raw materials there are a variety of ANFs, such as urase, trypsin inhibitor, phytic acid, agglutinin, lipoxidase and big
Beans antigen protein etc., influences the digestion of nutritional ingredient, absorbs and be metabolized, the health and production performance to animal body produce bad shadow
Ring, more effectively applied in fowl stores and raised so as to constrain vegetable protein.
The method of production high-efficiency biological protein feedstuff mainly includes protease hydrolyzed and microbial degradation at present.
There is the unrivaled advantage of many physics, chemical modification using enzymolysis protein feedstuff, it can not drop first
Low nutrition improves the functional characteristic of protein on the premise of being worth.Enzymolysis protein product is mainly peptide rather than amino acid, such as small
Peptide iron, antibacterial peptide, mucin peptide etc., the digestibility of these peptide matters are proven, than digesting and assimilating for amino acid
It is faster and better more complete, so as to significantly increase the digestive utilization ratio of dregs of beans, and vitamin potency can be protected.Next enzyme reaction
Mild condition, efficiency high does not produce racemization, does not also destroy amino acid.But enzymolysis dregs of beans exist a series of limitation because
Element, if the participation transformation without microorganism first in protein hydrolytic process, produced small peptide much carries bitter taste, agreeable to the taste
Property it is not good, in especially mass producing, reduction and bitter taste and stink in removing hydrolytic process need very high cost.Secondly
Solid digests forage protein, and enzyme amount addition is big, and production cost is high.
Lactic acid bacteria solid fermentation protein feed raw material, can increase the digestibility of protein during the fermentation, improve free
The content of amino acid, so as to improve dregs of beans nutritive value, meanwhile, most lactic acid bacterias can adjust animal intestinal tract dysfunction and
Maintain endogenous microecological balance.But traditional lactic acid bacteria can not generate gemma, the tolerance to high temperature, strong acid, highly basic and cholate
Property it is poor, can reach the viable count phase of enteron aisle after being prepared into micro-ecological feed and feeding animals through granulation, the process such as expanded
To less.Bacillus coagulans belongs to sclerine door enteron aisle lactic acid bacteria, is that a class can sporiferous special lactic acid bacteria.Except with biography
Lactic acid bacteria of uniting, which can adjust animal intestinal health, improves digestion power and improve immunity etc., acts on outer, and its gemma state is to environment
With suitable resistance, there is certain tolerance to high temperature, hydrochloric acid in gastric juice and cholate.The strain in 1992 by U.S.'s food with
Drug administration (FDA) and U.S. feed control official association are included in the safe strain panel available for feed.
In recent years, the ANFs and inhibitor in dregs of beans are removed using microbe fermentation method and enzymatic isolation method, beans are improved
Research in terms of the nutritive value of the dregs of rice is deep, also achieves some preferable achievements.But acted synergistically using bacterium, enzyme,
The research report for improving vegetable protein (based on dregs of beans) nutritive value and utilization rate is less.Feeding enzyme collaboration bacillus coagulans life
The method for producing High efficiency protein feed, has no relevant report at present.
The content of the invention
In order to overcome the shortcoming and deficiency of prior art, cooperateed with it is an object of the invention to provide a kind of with feeding enzyme
The method that bacillus coagulans produces High efficiency protein feed.Specifically a kind of bacillus coagulans collaboration complex enzyme for feed production is high
The method for imitating protein feed.
The present invention is feeding lactic acid bacteria using bacillus coagulans (Bacillus coagulans), except with biography
Lactic acid bacteria of uniting, which can adjust animal intestinal health, improves digestion power and improve immunity etc., acts on outer, and its gemma state is to environment
With suitable resistance, there is certain tolerance to high temperature, hydrochloric acid in gastric juice and cholate, it is former by biofermentation vegetable protein
Material, improves the dried organic acid of feed and lactic acid bacteria number.The present invention is produced using compound protease in enzymatic isolation method simultaneously
Feeding peptide, its peptide content and soluble protein content be high, and the trophism of product is good, and immunocompetence is strong.Using condensing gemma bar
The bitter peptides group for digesting generation is reconfigured and modified by bacterium fermentation process, and feed bitter taste is further reduced.The present invention
Microbial fermentation production and enzymolysis process are organically combined together, production cost is reduced, improve feed utilization rate and
Feed quality.
The purpose of the present invention is achieved through the following technical solutions：
It is a kind of that the method that bacillus coagulans produces High efficiency protein feed is cooperateed with feeding enzyme, comprise the following steps：Each group
Divide by mass percentage：
(1) bacillus coagulans seed liquor is prepared；
(2) bacillus coagulans Liquid Culture：Liquid Culture based formulas：Dusty yeast：15~30g/L, peptone：0~
10g/L, sodium chloride：1~5g/L, maltose：6~10g/L, starch：10~20g/L, magnesium sulfate：0.8~1.2g/L, sulfuric acid
Manganese：0.1~0.3g/L, disodium hydrogen phosphate：1~5g/L, sodium dihydrogen phosphate：0~5g/L, initial pH value：6.8~7.4；During culture
Fermentation tank liquid amount 20~60%, seed liquid measure 1~3%, 120~200r/min of rotating speed, 0.5~0.7m of throughput3/ h, 35~
39 DEG C of 15~20h of culture；
(3) bacillus coagulans solid state rheology：Material obtains bacterium solution according to 1 with step (2)：0.8~1：1.2 stirrings are equal
It is even, it is fitted into sterilization tank, seals, 36~42 DEG C is cultivated 2~3 days；
Described material is dregs of beans：Wheat bran=1:9；
(4) compost 0.4~1.0%, alkali protease (>=80U/mg) obtained by step (3) are not less than 0.2%, neutrality
It is 1 that protease (>=70U/mg), which is not less than 0.4%, acid protease (>=150U/mg) and is not less than 0.08%, material-water ratio,：0.6~
1：1, uniform mixing obtains dregs of beans mixture；
Material in described material-water ratio is dregs of beans：Wheat bran=9.8：0.2~9.4：0.6；
(5) the dregs of beans mixture obtained in step (4) is directly seated in fermentation cylinder for fermentation, sealing prevents moisture from dissipating
Hair；
(6) 37~42 DEG C of enzymatic hydrolysis and fermentations of collaboration, fermentation time 3~5 days are carried out after good seal；
(7) the good fermented bean dregs of the enzymatic hydrolysis and fermentation that obtains, can Direct-fed livestock and poultry.
(8) according to fermented bean dregs obtained by step (7), it is 10% to be dried under the conditions of 50~60 DEG C to moisture, is produced
High efficiency protein feed.
In order to which the present invention is better achieved,
Bacillus coagulans seed liquor described in step (1), is made by the steps and obtains：
The ring of picking bacillus coagulans inclined-plane 1, is inoculated into equipped with LB fluid nutrient mediums, 37 DEG C of shaking table, 180r/min is trained
6h is supported, bacillus coagulans seed liquor is made.
Bacillus coagulans described in step (1) is preferably bacillus coagulans GIM1.646, bacillus coagulans
GIM1.421, bacillus coagulans GW1.0042 or coagulated bacillus living piece etc.；More preferably bacillus coagulans
GIM1.646, bacillus coagulans GIM1.421 or bacillus coagulans GW1.0042.
The temperature of culture described in step (1) is preferably 37 DEG C；
The temperature of culture described in step (2) is preferably 37 DEG C；
Liquid Culture based formulas described in step (2)：Dusty yeast：25g/L, peptone：5g/L, sodium chloride：2g/L, wheat
Bud sugar：6g/L, starch：20g/L, magnesium sulfate：1.2g/L, manganese sulfate：0.3g/L, disodium hydrogen phosphate：3g/L, sodium dihydrogen phosphate：
5g/L, initial pH value：7.2；
Fermentation tank liquid amount 60% described in step (2), seed liquor inoculum concentration 1%, rotating speed 120r/min, air mass flow
0.6m2/ h, 37 DEG C of culture 15h；
Material described in step (3) is with bacterium solution according to 1：1, stir, be fitted into sterilization tank, seal, 37 DEG C of cultures 3
My god；
Step (4) considers financial cost and quality of finished, selection solid culture medium 0.8%, alkali protease (80U/mg)
0.2%th, neutral proteinase (70U/mg) 0.4%, acid protease (150U/mg) 0.08%, material (dregs of beans：Wheat bran=9.6：
0.4) water ratio：1：0.8；
39 DEG C of fermentation temperature described in step (6), fermentation time 4 days；
Being dried under the conditions of 55 DEG C to moisture described in step (8) is 10%.
A kind of High efficiency protein feed, is obtained by above-mentioned preparation method；
The stronger sour fragrance of High efficiency protein feed formation after described drying；PH is in 3.2~4.5, organic acid content
More than 2.8%；Acid-soluble protein improves more than 6.5 times before relatively fermenting, and soluble protein relative molecular weight is accounted within 500
More than 60%；Urease activity is less than 0.073U/g, and cotton seed sugared content is less than 0.42%, and stachyose is less than 0.035%；Condense bud
Spore lactic acid bacteria number is up to 5 × 109More than cfu/g.
The present invention mechanism be：In closed environment, at temperature and initial incubation pH value suitable condition, initial stage albumen raw material
Middle aerobic bacteria is using limited oxygen competitiveness growth, and after oxygen consumption, mould and oxygen consumption bacterium can not grow.Bacillus coagulans
Effectively acid-soluble protein and amino-acid nitrogen and the nothing that plant protein material (dregs of beans) is produced are digested using alkalescence and neutral protein
Oxygen environment turns into dominant bacteria, amount reproduction and generation organic acid (based on lactic acid), and pH reductions suppress the same of other varied bacteria growings
When there is provided acid protease digest environment, synergistic combination and modification bitter peptides and provide acid-soluble protein content, improve dry raise
The lactic acid bacteria and albumen quality of material.
The present invention has the following advantages and effect relative to prior art：
(1) bacillus coagulans is a kind of spore type lactic acid bacteria, is stablized with general lactic acid bacteria because it has, resistance
By force, the features such as resurrection rate is high, metabolism is vigorous.The materials such as bacteriocin, organic acid and hydrogen peroxide can be produced to suppress to cause in raw material
Pathogen growth, reduces the generation of amine harmful substance.
(2) gemma that bacillus coagulans is produced has stronger resistance, and feed drying process effectively preserves viable bacteria
Number, animal intake body, can improve immunity of organism and resistance against diseases, reduce the generation of intestines problem, improve the healthy water of animal
It is flat.
(3) alkali protease and neutral protease enzymolysis produce acid-soluble protein, promote the growth of bacillus coagulans.It is solidifying
Collect bacillus production acid, pH reductions digest environment there is provided acid protease, improve protein hydrolysis degree.Soluble protein
Relative molecular weight accounts for more than 60% within 500, avoids soya-bean polypeptides bitter taste most strong 500~1000D of relative molecular weight area
Domain.
(4) bacterium enzyme collaboration processing dregs of beans, without carrying out sterilization treatment, whole process amphimicrobian and anaerobism hair to dregs of beans
Ferment, energy consumption is small, and dry loss is low.The technological process of the present invention is easy to operate, has both been adapted to plant's directly production and has fed, has also fitted
Feed factory large-scale production is closed, meanwhile, cost of material is low, is adapted to industrialized production.
(5) fermented bean dregs of the invention produce a large amount of organic acids and beneficial bacterium during the fermentation, have stronger acid fragrant
Taste, palatability is strong.PH is in 3.2~4.5, organic acid content more than 2.8%；Acid-soluble protein improves more than 6.5 times before relatively fermenting,
Soluble protein relative molecular weight accounts for more than 60% within 500；Urease activity is less than 0.073U/g, and cottonseed sugared content is low
In 0.42%, stachyose is less than 0.035%；Lactic acid bacteria gemma number is condensed up to 2.0 × 109More than cfu/g.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
Implement as follows：Following components are by mass percentage
(1) prepared by separate sources bacillus coagulans seed liquor：The ring of picking bacillus coagulans inclined-plane 1, is inoculated into and is equipped with
In 100mL LB fluid nutrient mediums in 250mL triangular flasks, seed liquor is made in 37 DEG C of shaking table, 180r/min culture 6h；
(2) bacillus coagulans Liquid Culture：Culture medium prescription：Dusty yeast：15g/L, sodium chloride：5g/L, maltose：
8g/L, starch：15g/L, magnesium sulfate：0.8g/L, manganese sulfate：0.2g/L, disodium hydrogen phosphate：1g/L, sodium dihydrogen phosphate：3g/L,
Initial pH value：7.4.Fermentation tank liquid amount 40% during culture, seed liquid measure 3%, rotating speed 160r/min, throughput 0.6m3/ h, training
Support 35 DEG C of culture 18h；
(3) bacillus coagulans solid state rheology：Material (dregs of beans：Wheat bran=1:9) bacterium solution is obtained according to 1 with step (2)：
0.8 is stirred, and is fitted into sterilization tank, sealing, and 42 DEG C are cultivated 2 days；
(4) by solid culture medium 0.4%, alkali protease (80U/mg) 0.2%, neutral proteinase obtained by step (3)
(70U/mg) 0.4%, acid protease (150U/mg) 0.08%, material (dregs of beans：Wheat bran=9.8：0.2) water ratio：1：0.6, uniformly
Mixing, obtains dregs of beans mixture；
(5) the dregs of beans mixture obtained in step (4) is directly seated in fermentation cylinder for fermentation, sealing prevents moisture from dissipating
Hair；
(6) 37 DEG C of coordinated enzymatic hydrolysis fermentations, fermentation time 5 days are carried out after good seal.The fermented bean dregs fermented, can be with
Directly used directly as finished feed.
Obtained fermented bean dregs dry preceding as measurement result during finished feed and are shown in Table 1.Wherein, acid-soluble protein contains
Amount is according to QB/T 2653-2004 methods, and organic acid is measured by GB/T 5009.39-2003 methods, lactic acid bacteria bacterium number content
Measured according to GB 4789.35-2010 methods.
Obtained fermented bean dregs dry preceding as measurement result during finished feed and are shown in Table 1.
The measurement result of finished feed before table 1 is dried
Bacillus coagulans GIM1.646 described in table 1, bacillus coagulans GIM1.421, by Guangdong Province microorganism fungus kind
Collection is bought.
Bacillus coagulans GW1.0042 described in table 1, by Microbiology Research Inst., Guangzhou City, strain library is bought.
Coagulated bacillus living piece described in table 1 is commercially available prod.Name of product：Refreshing precious (Chinese medicines quasi-word of relaxing
S20050032)。
Embodiment 2
Implement as follows：Following components are by mass percentage
(1) prepared by bacillus coagulans (bacillus coagulans GIM1.646) seed liquor：Picking bacillus coagulans inclined-plane 1
Ring, is inoculated into equipped with 250mL triangular flasks in 100mL LB fluid nutrient mediums, kind is made in 37 DEG C of shaking table, 180r/min culture 6h
Sub- liquid；
(2) bacillus coagulans Liquid Culture：Culture medium prescription：Dusty yeast：30g/L, peptone：10g/L, sodium chloride：
1g/L, disodium hydrogen phosphate：5g/L, maltose, starch, magnesium sulfate, sulfuric acid manganese content design experiment (being shown in Table 2), initial pH value：
6.8.Fermentation tank liquid amount 20% during culture, seed liquid measure 2%, rotating speed 200r/min, throughput 0.6m3/ h, cultivates 39 DEG C of trainings
Support 20h；
The maltose of table 2, starch, magnesium sulfate, sulfuric acid manganese content design experiment
| Test number | A maltose (g/L) | B starch (g/L) | C magnesium sulfate (g/L) | D manganese sulfates (g/L) |
| 1 | 1(6) | 1(10) | 1(0.8) | 1(0.1) |
| 2 | 1(6) | 2(15) | 2(1.0) | 2(0.2) |
| 3 | 1(6) | 3(20) | 3(1.2) | 3(0.3) |
| 4 | 2(8) | 1(10) | 2(1.0) | 3(0.3) |
| 5 | 2(8) | 2(15) | 3(1.2) | 1(0.1) |
| 6 | 2(8) | 3(20) | 1(0.8) | 2(0.2) |
| 7 | 3(10) | 1(10) | 3(1.2) | 2(0.2) |
| 8 | 3(10) | 2(15) | 1(0.8) | 3(0.3) |
| 9 | 3(10) | 3(20) | 2(1.0) | 1(0.1) |
(3) bacillus coagulans solid state rheology：Material (dregs of beans：Wheat bran=1:9) bacterium solution is obtained according to 1 with step (2)：
1.2 are stirred, and are fitted into sterilization tank, sealing, and 36 DEG C are cultivated 3 days；
(4) by compost 1.0%, alkali protease (80U/mg) 0.2%, neutral proteinase (70U/ obtained by step (3)
Mg) 0.4%, acid protease (150U/mg) 0.08%, material (dregs of beans：Wheat bran=9.4：0.6) water ratio：1：1, uniform mixing is obtained
To dregs of beans mixture；
(5) the dregs of beans mixture obtained in step (4) is directly seated in fermentation cylinder for fermentation, sealing prevents moisture from dissipating
Hair；
(6) 42 DEG C of enzymatic hydrolysis and fermentations of collaboration, fermentation time 3 days are carried out after good seal；
(7) the good fermented bean dregs of the enzymatic hydrolysis and fermentation that obtains, can Direct-fed livestock and poultry.
(8) according to fermented bean dregs obtained by step (7), it is 10% to be dried under the conditions of 60 DEG C to moisture.Produce efficiently
Protein feed.
After obtained fermented bean dregs drying 3 are shown in Table as measurement result during finished feed.Embodiment is shown in organic acid measurement
1.Gemma bacterium number measuring method refers to GB 4789.2-2010, and wherein sample treatment is changed to take 25g samples to be placed in 225mL lifes
Reason salt solution boils 15min.Acid-soluble protein before acid-soluble protein/fermentation, acid-soluble after acid-soluble protein raising multiple=fermentation
Protein measurement method is shown in embodiment 1.Urease activity reduction=(urease activity after urease activity-fermentation before fermentation)/hair
Urease activity × 100% before ferment, urease activity is measured by GB/T 8622-2006.
The measurement result of finished feed after table 3 is dried
Embodiment 3
Implement as follows：Following components are by mass percentage
(1) prepared by bacillus coagulans (bacillus coagulans GIM1.646) seed liquor：Picking bacillus coagulans inclined-plane 1
Ring, is inoculated into equipped with 250mL triangular flasks in 100mL LB fluid nutrient mediums, kind is made in 37 DEG C of shaking table, 180r/min culture 6h
Sub- liquid；
(2) bacillus coagulans Liquid Culture：Culture medium prescription：Dusty yeast：25g/L, peptone：5g/L, sodium chloride：
2g/L, maltose：6g/L, starch：20g/L, magnesium sulfate：1.2g/L, manganese sulfate：0.3g/L, disodium hydrogen phosphate：3g/L, phosphoric acid
Sodium dihydrogen：5g/L, initial pH value：7.2.Fermentation tank liquid amount 60% during culture, seed liquid measure 1%, rotating speed 120r/min, ventilation
Measure 0.6m3/ h, cultivates 37 DEG C of culture 15h；
(3) bacillus coagulans solid state rheology：Material (dregs of beans：Wheat bran=1:9) bacterium solution is obtained according to 1 with step (2)：1
Stir, be fitted into sterilization tank, seal, 37 DEG C are cultivated 3 days；
(4) by compost 0.8%, alkali protease (80U/mg) 0.2%, neutral proteinase (70U/ obtained by step (3)
Mg) 0.4%, acid protease (150U/mg) 0.08%, material (dregs of beans：Wheat bran=9.6：0.4) water ratio：1：0.8, uniform mixing,
Obtain dregs of beans mixture；
(5) the dregs of beans mixture obtained in step (4) is directly seated in fermentation cylinder for fermentation, sealing prevents moisture from dissipating
Hair；
(6) 39 DEG C of enzymatic hydrolysis and fermentations of collaboration, fermentation time 3,4,5 days are carried out after good seal；
(7) the good fermented bean dregs of the enzymatic hydrolysis and fermentation that obtains, can Direct-fed livestock and poultry.
(8) according to fermented bean dregs obtained by step (7), it is 10% to be dried under the conditions of 55 DEG C to moisture.Produce efficiently
Protein feed.
After obtained fermented bean dregs drying 4, table 5 is shown in Table as measurement result during finished feed.Reality is shown in organic acid measurement
Apply example 1.Bacillus coagulans spore number measuring method, acid-soluble protein improve multiple and see embodiment 2.Urease activity is to pass through
GB/T 8622-2006 are measured.Raffinose, stachyose, soluble protein relative molecular weight distribution figure are by GPC-SEC methods
Measure.
The measurement result of finished feed before the secondary fermentation different number of days of table 4 is dried
The soluble protein relative molecular weight distribution figure of table 5
Application Example 1
The application of fermented bean dregs of the present invention：Carry out big pig and feed growth performance experiment.
The temperature and humidity of pig house：In daily 10 during experiment:00、14:00 determines the temperature and humidity of pig house, takes it to put down
Average is used as the temperature and humidity on the day of pig house；After measured, mean temperature is 30.52 ± 2.03 DEG C, put down in pig house during experiment
Equal humidity is 69.21 ± 2.53%.
Experimental animal：Body weight about 60kg Du × length × big hybridization galt 100；
Experimental animal is grouped：100 pigs are randomly divided into control group and test group, every group of 5 repetitions are each to repeat 10
Pig, is each repeated as 1 column, and single column area is 4.5m × 5.0m.
Feed：Control group fed routine daily ration, adds the grower pigs compound premix of mass fraction 4%, and daily ration used is used
Corn-soybean meal, with reference to NRC (1998) swine rearing standard preparation；Test group biofermentation material 35%, corn 33%, bran or
Coarse cereals 12%, dregs of beans or soya-bean cake 16%, live pig premix 4, biofermentation material is that the efficient protein prepared by embodiment 3 is raised
Material.The purchase of grower pigs compound premix is from sharp Bioisystech Co., Ltd of Beijing agricultural university, and other markets are purchased.
Respectively feed 1 time sooner or later daily, until stopping feeding；The nursing phase is 30 days；Experimental animal is raised by column, is freely drunk
Water, other feeding managements are routinely carried out.
After the nursing phase terminates, the growth performance index of determination experiment animal.Assay method is as follows：
Growth performance index determining：On-test and at the end of the night of fasting 1, it is each to repeat to being weighed by head animal
Feed feed intake individually record, calculate adding weight, daily ingestion amount, feed-weight ratio.
Experimental result, is shown in Table 6.
Influence of the fermented feed of table 6 to big pig production performance
P<0.01, show that difference is extremely notable；P<0.05, show significant difference；P>0.05, show that difference is not notable.
Application Example 2
The application of fermented bean dregs of the present invention：It is directly fed, nursing broiler chicken experiment is carried out.
Experimental animal：AA meat sold on the market baby chicks, 1000.
Experimental animal is grouped：1000 chickens are randomly divided into control group and test group, every group of 5 repetitions, each repeatedly 100
Chicken, is each repeated as 1 column.
Feed：Test group feeding formula：Corn 55.3%, dregs of beans 38%, calcium monohydrogen phosphate 1.4%, stone flour 1%, salt
0.3%, oil 3%, additive 1%.Biofermentation material is added according to the 4% of total amount.Biofermentation material is prepared into by embodiment 3
The High efficiency protein feed arrived.Control group is identical with test group nutritional ingredient, and feeding and management method is identical.
The nursing phase is 55 days, after the nursing phase terminates, the growth performance index of determination experiment animal.It is shown in Table 7.
Influence of the fermented bean dregs of table 7 to meat chicken growth performance
| Group | Average every quality (kg) | Feedstuff-meat ratio | Survival rate (%) |
| Control group | 2.08 | 2.57 | 94.1 |
| Test group | 2.43 | 2.14 | 97.2 |
High efficiency protein feed prepared by the present invention is directly fed after chicken, and hair color light, health is preferable, and control group
Hair color is substantially poor.The conversion ratio of feed is higher in feeding process, reduces the generation of disease, with higher survival rate.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, it is characterised in that includes following step
Suddenly：Each component is by mass percentage：
(1) bacillus coagulans seed liquor is prepared；
(2) bacillus coagulans Liquid Culture：Liquid Culture based formulas：Dusty yeast：15~30g/L, peptone：0~10g/L,
Sodium chloride：1~5g/L, maltose：6~10g/L, starch：10~20g/L, magnesium sulfate：0.8~1.2g/L, manganese sulfate：0.1~
0.3g/L, disodium hydrogen phosphate：1~5g/L, sodium dihydrogen phosphate：0~5g/L, initial pH value：6.8~7.4；Fermented during culture canned
Liquid measure 20~60%, seed liquid measure 1~3%, 120~200r/min of rotating speed, 0.5~0.7m of throughput3/ h, 35~39 DEG C of cultures
15~20h；
(3) bacillus coagulans solid state rheology：Material obtains bacterium solution according to 1 with step (2)：0.8~1：1.2 stir, dress
Enter in sterilization tank, seal, 36~42 DEG C are cultivated 2~3 days；
Described material is dregs of beans：Wheat bran=1:9；
(4) compost 0.4~1.0%, alkali protease obtained by step (3) are not less than 0.2%, neutral proteinase and be not less than
0.4%th, it is 1 that acid protease, which is not less than 0.08%, material-water ratio,：0.6~1：1, uniform mixing obtains dregs of beans mixture；
Material in described material-water ratio is dregs of beans：Wheat bran=9.8：0.2~9.4：0.6；
(5) the dregs of beans mixture obtained in step (4) is directly seated in fermentation cylinder for fermentation, sealing prevents moisture from distributing；
(6) 37~42 DEG C of enzymatic hydrolysis and fermentations of collaboration, fermentation time 3~5 days are carried out after good seal；
(7) the good fermented bean dregs of the enzymatic hydrolysis and fermentation that obtains.
2. according to claim 1 cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, it is special
Levy and be also to include：
(8) according to fermented bean dregs obtained by step (7), it is 10% to be dried under the conditions of 50~60 DEG C to moisture, is produced efficiently
Protein feed.
3. according to claim 1 or 2 cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, its
It is characterised by：
Bacillus coagulans described in step (1) is bacillus coagulans GIM1.646, bacillus coagulans GIM1.421, coagulated
Tie bacillus GW1.0042 or coagulated bacillus living piece.
4. according to claim 1 or 2 cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, its
It is characterised by：
Liquid Culture based formulas described in step (2)：Dusty yeast：25g/L, peptone：5g/L, sodium chloride：2g/L, malt
Sugar：6g/L, starch：20g/L, magnesium sulfate：1.2g/L, manganese sulfate：0.3g/L, disodium hydrogen phosphate：3g/L, sodium dihydrogen phosphate：5g/
L, initial pH value：7.2.
5. according to claim 1 or 2 cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, its
It is characterised by：
Fermentation tank liquid amount 60% described in step (2), seed liquor inoculum concentration 1%, rotating speed 120r/min, air mass flow
0.6m2/ h, 37 DEG C of culture 15h.
6. according to claim 1 or 2 cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, its
It is characterised by：
Material described in step (3) is with bacterium solution according to 1：1, stir, be fitted into sterilization tank, seal, 37 DEG C are cultivated 3 days.
7. according to claim 1 or 2 cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, its
It is characterised by：
In step (4), selection solid culture medium 0.8%, alkali protease 0.2%, neutral proteinase 0.4%, acid protease
0.08%th, material-water ratio：1：0.8；
Material in described material-water ratio is dregs of beans：Wheat bran=9.6：0.4.
8. according to claim 1 or 2 cooperate with the method that bacillus coagulans produces High efficiency protein feed with feeding enzyme, its
It is characterised by：
39 DEG C of fermentation temperature described in step (6), fermentation time 4 days.
9. a kind of High efficiency protein feed, it is characterised in that obtained by the preparation method described in any one of claim 1~8.
10. High efficiency protein feed according to claim 9, it is characterised in that：
The stronger sour fragrance of High efficiency protein feed formation after described drying；PH 3.2~4.5, organic acid content 2.8% with
On；Acid-soluble protein improves more than 6.5 times before relatively fermenting, and soluble protein relative molecular weight accounts for more than 60% within 500；
Urease activity is less than 0.073U/g, and cotton seed sugared content is less than 0.42%, and stachyose is less than 0.035%；Condense lactic acid bacteria
Count up to 5 × 109More than cfu/g.
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| CN108713634A (en) * | 2018-06-14 | 2018-10-30 | 广东希普生物科技股份有限公司 | High peptide fermented bean dregs of peracid and preparation method thereof |
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| CN113604404A (en) * | 2021-08-31 | 2021-11-05 | 四川润格生物科技有限公司 | Bacillus coagulans YSF17 and application thereof |
| CN113604404B (en) * | 2021-08-31 | 2022-08-16 | 四川润格生物科技有限公司 | Bacillus coagulans YSF17 and application thereof |
| CN115517315A (en) * | 2022-09-23 | 2022-12-27 | 江苏福润德科技有限公司 | Protein feed fermentation process |
| CN116965482A (en) * | 2023-08-25 | 2023-10-31 | 山东和众康源生物科技有限公司 | Method for preparing soybean enzymolysis protein by biological fermentation and application thereof |
| CN116965482B (en) * | 2023-08-25 | 2024-03-15 | 山东和众康源生物科技有限公司 | Method for preparing soybean enzymolysis protein by biological fermentation and application thereof |
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