CN106963985B

CN106963985B - Decellularization net cage and decellularization device

Info

Publication number: CN106963985B
Application number: CN201710402171.0A
Authority: CN
Inventors: 米维; 雷文诚; 王红刚; 韩卫东
Original assignee: Shaanxi Ruisheng Biotech Co ltd
Current assignee: Shaanxi Ruisheng Biotech Co ltd
Priority date: 2017-05-31
Filing date: 2017-05-31
Publication date: 2020-09-18
Anticipated expiration: 2037-05-31
Also published as: CN106963985A

Abstract

The invention discloses a decellularization net cage and a decellularization device, and relates to the technical field of biological material treatment devices. The invention aims to solve the problems that the cell removing device in the prior art cannot adapt to mass production and has complex installation and disassembly processes. The invention relates to a decellularization net cage which comprises an outer layer box body and an inner layer box body, wherein the outer layer box body is used for being connected with a shaking table, the inner layer box body can synchronously oscillate along with the outer layer box body, a plurality of cleaning grooves used for placing biological materials and a decellularization reagent are formed in the inner layer box body, the outer layer box body is connected with a liquid discharge pipe, the liquid discharge pipe is communicated with the bottom space of the outer layer box body, each cleaning groove is provided with a liquid discharge hole, the liquid discharge holes are communicated with the bottom space of the outer layer box body, and the liquid discharge holes allow the decellularization reagent to pass through. The acellular net cage can be used for acellular biological materials.

Description

Decellularization net cage and decellularization device

Technical Field

The invention relates to the technical field of biological material treatment devices, in particular to a decellularization net cage and a decellularization device.

Background

With the development of economy and society, the medical application of biomaterials is also more and more prominent, and biomaterials refer to materials formed by biological processes, including cells and extracellular matrix. Common biological materials include bovine pericardium and porcine small intestine, and when the biological materials are applied to clinic, the biological materials need to be cleaned, sterilized, disinfected, decellularized and the like.

In the prior art, a shaking table is usually used for decellularization, specifically, a cleaning box is fixed on a tray of the shaking table, biological materials and a decellularization reagent are put into the cleaning box, then the shaking table is started to oscillate, and during the oscillation process, the inner wall of the cleaning box repeatedly slaps the biological materials, thereby realizing the decellularization of the biological materials.

However, the volume of the washing box cannot be made large due to the limitation of the oscillation amplitude of the shaking table, and if the volume is too large, the distance between the two opposite side walls of the washing box is large, so that the biological material moves in the opposite direction without slapping against the inner wall of the washing box in the oscillation process of the washing box, and the cell removal cannot be realized. Therefore, the prior art decellularization cleaning cartridge is generally small in volume to adapt to the oscillation amplitude of the shaking table, and cannot adapt to mass production. If fix a plurality of independent washing boxes simultaneously on the tray of shaking table, then when discharge liquid (taking off cell reagent promptly), need will wash the box and demolish the back one by one and pour the liquid in the box of washing, the installation is more complicated with the dismantlement process to lead to production efficiency to descend.

Disclosure of Invention

The embodiment of the invention provides a decellularization net cage and a decellularization device, which can increase the treatment quantity of biological materials, and have the advantages of simple liquid drainage operation and high production efficiency.

In order to achieve the above object, an embodiment of the present invention provides a decellularization net cage for decellularizing a biological material, including an outer layer box and an inner layer box arranged in the outer layer box, wherein the outer layer box is used for being connected with a shaking table, the inner layer box can synchronously oscillate along with the outer layer box, a plurality of cleaning tanks for placing the biological material and a decellularization reagent are formed in the inner layer box, the outer layer box is connected with a liquid discharge pipe, the liquid discharge pipe is communicated with a bottom space of the outer layer box, each cleaning tank is provided with a liquid discharge hole, the liquid discharge hole is communicated with the bottom space of the outer layer box, and the liquid discharge hole allows the decellularization reagent to pass through and does not allow the biological material to pass through.

The invention also provides a cell removing device which comprises a shaking table, wherein the shaking table comprises a swinging tray, and the swinging tray is provided with the cell removing net cage in any technical scheme.

According to the decellularization net cage and the decellularization device, the outer layer box body is used for being connected with the shaking table, the inner layer box body can synchronously oscillate along with the outer layer box body, so that the inner layer box body can synchronously oscillate along with the outer layer box body when the outer layer box body oscillates, the decellularization treatment of biological materials in the inner layer box body is achieved, and the plurality of cleaning grooves used for placing the biological materials and the decellularization reagent are formed in the inner layer box body, so that the volume of each cleaning groove can be made to be the maximum volume suitable for the oscillation amplitude of the shaking table, and compared with one cleaning box in the prior art, the treatment quantity of the biological materials is increased. And because outer box is connected with the fluid-discharge tube, a plurality of washing tanks all are equipped with the outage, consequently when the flowing back, only need open the fluid-discharge tube, the reagent of taking off the cell in the washing tank can be discharged by the fluid-discharge tube, need not to dismantle outer box and inlayer box, easy operation, and production efficiency is high.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of the overall structure of a decellularization net cage according to an embodiment of the invention;

FIG. 2 is a schematic view of the internal structure of the decellularization net cage according to the embodiment of the invention;

FIG. 3 is a schematic structural diagram of an outer box of the decellularization net cage according to the embodiment of the invention;

FIG. 4 is a schematic structural diagram of an inner-layer box body of the decellularization net cage according to the embodiment of the invention;

FIG. 5 is a schematic bottom structure view of an inner box of the decellularization net cage according to the embodiment of the invention;

FIG. 6 is a schematic structural diagram of a decellularization net cage with a liquid adding device and a bubble absorbing device according to an embodiment of the invention;

FIG. 7 is a schematic structural diagram of another embodiment of a bubble-absorbing device of the decellularization net cage according to the embodiment of the invention;

fig. 8 is a schematic structural diagram of an inner-layer box cover of the decellularization net cage according to the embodiment of the invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

Referring to fig. 1 and 2, fig. 1 and 2 are specific embodiments of a decellularization net cage according to an embodiment of the present invention, the decellularization net cage according to the embodiment of the present invention is used for decellularizing a biological material, and includes an outer layer box 1 and an inner layer box 2 disposed in the outer layer box 1, the outer layer box 1 is used for being connected to a shaking table 3, the inner layer box 2 can oscillate synchronously with the outer layer box 1, a plurality of cleaning tanks 21 for placing the biological material and a decellularization reagent are formed in the inner layer box 2, the outer layer box 1 is connected to a liquid discharge pipe 11, the liquid discharge pipe 11 is communicated with a bottom space of the outer layer box 1, each cleaning tank 21 is provided with a liquid discharge hole 211, the liquid discharge holes 211 are communicated with the bottom space of the outer layer box 1, and the liquid discharge holes 211 allow the decellularization reagent to pass through.

According to the acellular net cage provided by the embodiment of the invention, the outer layer box body 1 is used for being connected with the shaking table, the inner layer box body 2 can synchronously oscillate along with the outer layer box body 1, so that when the outer layer box body 1 oscillates, the inner layer box body 2 can synchronously oscillate along with the outer layer box body 1, and the acellular treatment of the biological materials in the inner layer box body 2 is realized, and the plurality of cleaning grooves 21 for placing the biological materials and the acellular reagents are formed in the inner layer box body 2, so that the volume of each cleaning groove 21 can be made to be the maximum volume suitable for the oscillation amplitude of the shaking table, and compared with one cleaning box in the prior art, the treatment quantity of the biological materials is. And because outer box 1 is connected with fluid-discharge tube 11, a plurality of washing tanks 21 all are equipped with outage 211, consequently when flowing back, only need open fluid-discharge tube 11, the reagent of taking off cells in the washing tank 21 can be discharged by fluid-discharge tube 11, need not to dismantle outer box 1 and inlayer box 2, easy operation, production efficiency is high.

Wherein, in order to realize inlayer box 2 along with outer box 1's synchronous oscillation, can be with the inside dimension of outer box 1 and the outside dimension phase-match of inlayer box 2, when inlayer box 2 installs in outer box 1 promptly, laminate each other between the inside wall of outer box 1 and the lateral wall of inlayer box 2 to make outer box 1 at the in-process of oscillation, inlayer box 2 can't remove for outer box 1, avoid producing the striking between outer box 1 and the inlayer box 2. In addition, also can set up location structure between outer box 1 and inlayer box 2, prevent through location structure that inlayer box 2 from removing for outer box 1, for example can set up the rubber pad between outer box 1 and inlayer box 2, be fixed in on the inside wall of outer box 1 with the one end of rubber pad, the other end supports with the lateral wall of inlayer box 2 and leans on, or is fixed in on the lateral wall of inlayer box 2 with the one end of rubber pad, the other end supports with the inside wall of outer box 1 and leans on. Synchronous oscillation of the inner box body 2 along with the outer box body 1 can be realized.

As shown in fig. 4, handles 22 are disposed on two sides of the upper surface of the inner case 2, so that the inner case 2 can be conveniently taken out from the outer case 1, and the inner case 2 can be conveniently sterilized and cleaned.

Specifically, the inner casing 2 may have a structure as shown in fig. 4 and 5, that is, the inner casing 2 includes a bottom plate 23 and a side plate 24 disposed around the bottom plate 23, a partition 25 is disposed in the inner casing 2, and the partition 25 partitions a space in the inner casing 2 into a plurality of the cleaning tanks 21. In addition, a plurality of independent cleaning tanks 21 can be directly placed in the inner layer box body 2 without adopting the partition plate 25, and the outer side walls of the plurality of cleaning tanks 21 are adhered to each other and closely arranged in the inner layer box body 2. The solution shown in fig. 4 is simple in structure and has low requirements on manufacturing accuracy, and therefore is relatively more economical and practical.

The arrangement of the plurality of cleaning tanks 21 may be various, for example, the plurality of cleaning tanks 21 may be arranged in a row along the same line, or the plurality of cleaning tanks 21 may be arranged in a rectangular array. Compared with the washing tank along a linear array, the washing tank according to the rectangular array is more compact in overall structure and is not easy to deform during oscillation. For example, as shown in fig. 1, the partition plate 25 divides the space inside the inner casing 2 into 16 washing tanks, and the 16 washing tank arrays are 4 rows and 4 columns. Similarly, the partition plate 25 may divide the space in the inner casing 2 into 9 washing tanks, and the 9 washing tank arrays are 3 rows and 3 columns. The specific division and arrangement can be selected according to actual situations, and is not limited herein.

For facilitating the filling, as shown in fig. 4, a communication hole 251 is preferably formed at the lower end of the partition plate 25, and two adjacent cleaning tanks 21 are communicated through the communication hole 251. Thus, when the cell-removing reagent is supplied, it is not necessary to supply the cell-removing reagent into the cleaning tanks 21 one by one, and the cell-removing reagent is supplied into one of the cleaning tanks 21 and flows into the other cleaning tank 21 through the communication hole 251. Moreover, the dosage and the concentration of the cell removing reagent in each cleaning tank 21 can be equal by the scheme, so that the cell removing effect of the biological materials cleaned in the same batch is consistent.

As shown in fig. 4, the communication holes 251 are provided around the circumference of the cleaning tank 21, and are provided in two rows in the vertical direction. The communication holes 251 are not required to be formed too much, 1-3 rows are preferably arranged along the vertical direction, if the communication holes are arranged too much, the slapping force of the partition plate 25 to the liquid is reduced, the movement speed of the liquid driving the biological material is reduced, the slapping force between the biological material and the partition plate 25 is reduced, and the cell removing effect is influenced.

In an embodiment of the present invention, as shown in fig. 5, the liquid discharge holes 211 are distributed on the bottom plate 23 of the inner tank 2 and respectively correspond to the plurality of cleaning tanks 21, a communication cavity 28 is formed between the bottom plate 23 of the inner tank 2 and the bottom plate 12 of the outer tank 1, and the plurality of liquid discharge holes 211 on the bottom plate 23 of the inner tank 2 are all communicated with the communication cavity 28. Therefore, when the liquid discharge pipe 11 is opened during liquid discharge, the liquid in the cleaning tank 21 is discharged through the communicating cavity 28 and the liquid discharge pipe 11 in sequence, the operation is convenient, and the inner-layer box body 2 does not need to be detached.

In the above embodiment, the communication chamber 28 can be implemented in various ways, for example, as shown in fig. 2, a support member 27 can be disposed between the bottom plate 23 of the inner case 2 and the bottom plate 12 of the outer case 1, and the support member 27 can separate the bottom plate 23 of the inner case 2 from the bottom plate 12 of the outer case 1, so that the communication chamber 28 is formed between the bottom plate 23 of the inner case 2 and the bottom plate 12 of the outer case 1. Similarly, a hanging structure can be arranged between the side wall of the inner box 2 and the side wall of the outer box 1, so that the inner box 2 is hung in the outer box 1, and a communication cavity 28 is formed between the bottom plate 23 of the inner box 2 and the bottom plate 12 of the outer box 1.

In another embodiment of the present invention, the liquid discharge holes 211 may also be distributed on the side plate 24 of the inner casing 2, as shown in fig. 4, a communication hole 251 is formed at the lower end of the partition plate 25, two adjacent cleaning tanks 21 are communicated through the communication hole 251, and the liquid discharge hole 211 is formed at the lower end of the side plate 24. Therefore, when the liquid discharge pipe 11 is opened during liquid discharge, the liquid in the cleaning tank 21 positioned in the middle flows into the cleaning tank 21 on the outermost side through the communication hole 251, then is discharged into the outer tank 1 through the liquid discharge hole 211 at the lower end of the side plate 24, and finally is discharged through the liquid discharge pipe 11, and the inner tank 2 does not need to be detached.

The two arrangements of the drain holes 211 may be one arrangement or two arrangements may be used simultaneously. When only the first embodiment is adopted and the drain holes 211 are formed in the bottom plate 23 of the inner casing 2, the drain holes 211 are easily clogged with the biomaterial and the drainage effect is not good, so that it is preferable to adopt both embodiments.

The diameter of the drain hole 211 is preferably 3 mm, and if the diameter is too large, the biological material is easily stuck, and if the diameter is too small, the drain speed is too slow.

The horizontal section of the cleaning tank 21 may be rectangular, square, polygonal circular, etc., and is not limited herein. Since the oscillation amplitude of the shaking table 3 is 30 to 60 mm, it is preferable to set the maximum dimension of the washing tank 21 in the horizontal direction to be less than or equal to 300 mm in order to ensure that the washing tank 21 can be adapted to the oscillation amplitude of the shaking table 3. For example, when the cleaning tank 21 has a rectangular shape, the width of the cleaning tank 21 may be set to 140 mm and the length thereof may be set to 230 mm.

Preferably, the height of the cleaning tank 21 in the vertical direction should be greater than or equal to 100 mm, for example, the height may be selected to be 250 mm, so that the liquid in the cleaning tank 21 can be prevented from splashing when oscillating.

In order to add the decellularization reagent into the cleaning tank 21 conveniently, as shown in fig. 6, a liquid adding device 4 can be further provided, the liquid adding device 4 comprises a liquid storage tank 41, a liquid adding pipe 42 and a liquid adding pump 43, one end of the liquid adding pipe 42 is communicated with the liquid storage tank 41, the other end of the liquid adding pipe extends into the cleaning tank 21, and the liquid adding pump 43 is connected with the liquid adding pipe 42 in series; a liquid level meter (not shown in the figure) is also arranged and is used for detecting the liquid level in the cleaning tank 21; the liquid adding device controls liquid adding through a control unit, the control unit is respectively connected with the liquid adding pump 43 and the liquid level meter, and when the liquid level detected by the liquid level meter is lower than a preset value, the control unit controls the liquid adding pump 43 to be started. Therefore, when the liquid level detected by the liquid level meter is lower than the preset value, the control unit can control the liquid adding pump 43 to be started, pump the decellularized reagent in the liquid storage tank 41 into the cleaning tank 21, and close the liquid adding pump 43 when the specified liquid level height is reached. Therefore, automatic liquid supplementing is realized, the added reagent amount can be accurately controlled, and the working efficiency is improved.

It should be noted that fig. 6 is only for showing the connection relationship between the components of the liquid adding device 4 and the outer-layer tank, and does not limit the relative positional relationship between the liquid adding device 4 and the outer-layer tank, and the liquid adding device 4 may be disposed on any side of the outer-layer tank.

Since the cell removal reagent generates foam during the cell removal process, and the foam accumulation may affect the measurement accuracy of the liquid level meter and also affect the cell removal effect, as shown in fig. 6, it is preferable that an inner tank cover 26 is disposed at an opening of the inner tank 2, a bubble suction device 5 is disposed on the inner tank cover 26, and the bubble suction device 5 is respectively communicated with upper spaces of the plurality of cleaning tanks 21 for sucking foam generated by the cell removal reagent in the cleaning tanks 21. Therefore, the foam can be sucked by the foam sucking device 5, and the excessive foam accumulation is prevented.

Specifically, the bubble absorbing device 5 may be the structure shown in fig. 7, the bubble absorbing device 5 includes a plurality of bubble absorbing pipes 51, the plurality of bubble absorbing pipes 51 are disposed through the inner tank cover 26, a plurality of first ends of the bubble absorbing pipes 51 are extended into the plurality of cleaning tanks 21 in a one-to-one correspondence manner, a plurality of second ends of the bubble absorbing pipes 51 are communicated with the collecting container 52, and the plurality of bubble absorbing pipes 51 are respectively connected in series with a suction pump 53. Thus, the plurality of suction pumps 53 can control the plurality of suction bubble tubes 51, respectively, and the control accuracy is high.

In another embodiment of the present invention, as shown in fig. 6, the bubble absorbing device 5 includes a plurality of bubble absorbing tubes 51, the bubble absorbing tubes 51 are disposed through the inner case cover 26, first ends of the bubble absorbing tubes 51 are extended into the plurality of cleaning grooves 21 in a one-to-one correspondence manner, second ends of the bubble absorbing tubes 51 are communicated with a collecting tube 55 through a multi-way tube joint 54, the collecting tube 55 is communicated with the collecting container 52, and the collecting tube 55 is connected in series with a suction pump 53. Thus, only one suction pump 53 is required to suck the bubbles from all the cleaning tanks 21, which saves cost and space.

In order to facilitate the liquid discharge, the bottom plate 12 of the outer casing 1 may be inclined, and the lowest point of the bottom plate 12 of the outer casing 1 may be disposed near the drain pipe 11. Therefore, the decellularization reagent in the outer casing 1 can flow to the inlet of the liquid discharge pipe 11 along the inclined surface, and the remaining of the decellularization reagent at the bottom of the outer casing 1 can be prevented.

In order to avoid the above problem, it is preferable that the inner cover 26 is configured as a flat plate-like structure, the upper edges of the side plate 24 and the partition plate 25 are located in the same plane, and the inner cover 26 is covered on the side plate 24 and the partition plate 25. To facilitate access to the inner cover 26, a cover handle 261 may be provided on the inner cover 26, as shown in fig. 8.

In order to facilitate the observation of the inside of the washing tub 21, the inner case cover 26 is preferably made of a transparent material. Therefore, the conditions in the cleaning tank 21, such as the amount of foam, the level of liquid, the state of biological materials, etc., can be observed through the inner tank cover 26. If abnormal conditions occur, the abnormal conditions can be found in time.

In addition, as shown in fig. 3, an outer case cover 13 may be disposed at an opening of the outer case 1, so as to prevent foreign substances from entering the decellularization reagent and contaminating the biological material.

Referring to fig. 1, an embodiment of the present invention further provides a decellularization device, including a shaking table, where the shaking table includes a swing tray (not shown in the figure), and the swing tray is provided with a decellularization net cage according to any one of the above technical solutions.

Since the decellularization net cage used in the decellularization device of the embodiment is the same as the decellularization net cages provided in the embodiments of the decellularization net cage, the decellularization net cage and the decellularization net cage can solve the same technical problem and achieve the same expected effect.

Specifically, the outer layer box 1 of the decellularization net cage is clamped and fixed on the swing tray through a clamping structure, as shown in fig. 1, the bottom surface of the shaking table is provided with a retractable roller 31, which is convenient for carrying the shaking table.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims

1. A decellularization net cage is used for decellularizing biological materials and is characterized by comprising an outer layer box body and an inner layer box body arranged in the outer layer box body, wherein the outer layer box body is used for being connected with a shaking table, the inner layer box body can synchronously oscillate along with the outer layer box body, a plurality of cleaning grooves used for placing the biological materials and the decellularization reagent are formed in the inner layer box body, the outer layer box body is connected with a liquid discharge pipe, the liquid discharge pipe is communicated with the bottom space of the outer layer box body, each cleaning groove is provided with a liquid discharge hole, the liquid discharge holes are communicated with the bottom space of the outer layer box body, and the liquid discharge holes allow the decellularization reagent to pass through and do not;

the inner-layer box body comprises a bottom plate and side plates arranged around the bottom plate in a circle, a partition plate is arranged in the inner-layer box body, and the space in the inner-layer box body is divided into a plurality of cleaning tanks by the partition plate; the lower end of the partition board is provided with a communicating hole, and two adjacent cleaning tanks are communicated through the communicating hole.

2. The acellular cage according to claim 1, characterized in that the drainage holes are multiple, at least a part of the drainage holes are arranged on the bottom plate of the inner box body, a communicating cavity is formed between the bottom plate of the inner box body and the bottom plate of the outer box body, and the drainage holes on the bottom plate of the inner box body are all communicated with the communicating cavity.

3. The acellular cage according to claim 2, characterized in that at least a part of the drainage holes are opened at the lower end of the side plate.

4. The acellular cage according to claim 1, wherein the maximum dimension of the cleaning tank in the horizontal direction is less than or equal to 300 mm, and the height of the cleaning tank in the vertical direction is greater than or equal to 100 mm.

5. The acellular net cage according to any one of claims 1 to 3, wherein the bottom plate of the outer box body is obliquely arranged, and the lowest point of the bottom plate of the outer box body is arranged close to the drain pipe.

6. The decellularization net cage according to claim 2, wherein an inner box cover is arranged at an opening of the inner box body, the inner box cover is of a flat plate-shaped structure, the upper edges of the side plates and the partition plates are located in the same plane, and the inner box cover is covered on the side plates and the partition plates.

7. The acellular net cage according to claim 6, wherein the inner-layer box cover is provided with a bubble absorbing device, the bubble absorbing device comprises a plurality of bubble absorbing pipes, the bubble absorbing pipes penetrate through the inner-layer box cover, first ends of the bubble absorbing pipes correspondingly extend into the cleaning grooves one by one, second ends of the bubble absorbing pipes are communicated with a collecting pipe through a multi-way pipe joint, and the collecting pipe is connected with a suction pump in series.

8. A decellularization device, which is characterized by comprising a shaking table, wherein the shaking table comprises a swing tray, and the decellularization net cage as claimed in any one of claims 1 to 7 is arranged on the swing tray.