CN106962177A - A kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type - Google Patents
A kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type Download PDFInfo
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- CN106962177A CN106962177A CN201710251284.5A CN201710251284A CN106962177A CN 106962177 A CN106962177 A CN 106962177A CN 201710251284 A CN201710251284 A CN 201710251284A CN 106962177 A CN106962177 A CN 106962177A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type, including：Using high-resistance starch rice varieties as parent, with high zinc mixing breed, hybridize to obtain F1For seed；F1And BC1F1, it is returned respectively with high zinc kind, obtains BC2F1For seed；Plant BC1F1Plant, double rate is built using anther culture technique, and DH is harvested by individual plant0For seed；DH1~2Pest-resistant and 2 nutrient characteristics polymerizations of high zinc content the individual plant of type is walked quickly and kept away in generation, selection；DH3Generation, selection breeding time is no more than 135 days ripe phases moderate strain；DH4Generation, seed selection sizing strain obtains walking quickly and keeping away the pest-resistant and high zinc infant safe nutrition rice varieties of type；2 kinds of good characteristics of the pest-resistant and high zinc of type are walked quickly and kept away in present invention polymerization, take the technology that secondary Anther Culture again is returned with parent, the problem of overcoming indica type Anther Culture hardly possible, are walked quickly and kept away the pest-resistant and high zinc of type to set up big mass screening and are laid a good foundation.
Description
Technical field
The present invention relates to rice breeding technology field, especially a kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type.
Background technology
Zinc is micronutrient element needed by human, with important physiological action and trophic function.It not only with human body
Interior a variety of enzymatic activitys are relevant, also assist in the synthesis of the large biological molecules such as protein, nucleic acid.Human body zinc-deficiency can cause a series of generation
Thank to disorder, directly endanger human health, such as child's zinc-deficiency gently can then cause growth retardation, immunity function reduction, it is heavy then can
Cause intelligence development obstacle, in addition it is dead.At present, the population in the whole world nearly 50% is influenceed by zinc deficiency, the population of Asia 61%
There is the not enough risk of zinc intake, the whole world there are nearly 800,000 children lethal because of zinc-deficiency every year, and this trend is not still
Disconnected aggravation.As can be seen here, zinc turns into the global important element of influence human health.
Zn-ef ficiency is primarily present in marine product, pluck, wherein it is the highest containing zinc with oyster, zinc is contained in other foods
Amount is seldom.In diet, the whether staple food such as rice, noodles and bread, or children's love eat milk, eggs food and
Other snacks are nearly all without zinc, and vegetables and fruit rich in zinc are also seldom.Although the content of zinc is higher in coarse food grain, due to richness
Containing the typical ANFs such as phytic acid, the zinc that can be digested and absorbed is still very low.The World Health Organization and the United Nations's grain group
Suggestion is knitted, the intake of daily zinc should be more than 15mg.According to detection, animal food zinc content is relatively more, per 100g about
Containing 3~5mg of zinc；It is less containing zinc in vegetable food, per 100g containing about zinc 1mg.Therefore, it is difficult to meet zinc according to conventional diet
Demand, very necessary adjustment dietary structure, from the food rich in zinc to improve the shortage of zinc.
The reason for causing zinc insufficiency of intake from food, main the following aspects：One the reason for be kind, common grain
Food, fruits and vegetables kind do not possess the ability for being absorbed from soil, synthesizing, operating zinc in itself, cause the natural of agricultural product to lack
Zinc；Two be fertilization mode, and current implantation methods largely apply chemical fertilizer, further limit the utilization of zinc in soil, the grain of production
Eat universal zinc-deficiency；Three be that food process is too refined, and consumer is reluctant to sacrifice mouthfeel, causes the content of zinc in food to reduce.Rice
Meal is the main source of the staple food, even more nutrition and energy of our people's three meals in a day.Zinc in presently commercially available general rice
Content is only averagely 10mg/Kg, and the demand of zinc can not possibly be met by normally having enough.Therefore, carry out high zinc rice breeding, implement daily
Diet strategy meets the demand of zinc nutrition, significant to mitigation and the present situation for improving zinc deficiency.
As Global climate change, agricultural structure adjustment and cultivar are changed, the biotic that Rice Production faces
It is increasingly severe.Two, which move original pest and disease damage such as insect planthopper and striped rice borer, frequently breaks out, and causes huge economic loss, because pre-
It is anti-to cause very universal to rice yield and health and the serious harm of security quality not in time.Therefore, pest-resistant water is strengthened
Rice varieties seed selection, contributes to the environment-friendly production of paddy rice, promotes the development of green and ecological organic agricultural.
2 kinds of good characteristics of the pest-resistant and high zinc of type are walked quickly and kept away in purport polymerization of the present invention, cultivate the safety of particularly suitable infant
Nutrition spy's meals rice varieties.
The content of the invention
The invention provides a kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type, pyramiding breeding strategy is taken, is bred as
Sizing strain, 2 kinds of good characteristics for walking quickly and keeping away the pest-resistant and high zinc of type take into account, and can not only prevent and control the hair of pregnant woman's diabetes
It is raw, zinc nutrition can be effectively supplemented again promotes growing for baby.
The technical problems to be solved by the invention are realized by following technical scheme：
A kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type, including：
(1) selection walks quickly and keeps away type insect-proof rice kind for parent I, and high zinc content paddy rice is parent II, is hybridized, and obtains F1
Seed；
(2) F is planted1In generation, it is returned 1 time by male parent of parent II, obtains BC1F1For seed；
(3) BC is planted1F1In generation, it is returned again as male parent 1 time using parent II, obtains BC2F1Seed；
(4) BC is planted2F1Plant, doubled haploid (DH) colony is built using anther culture technique, and DH is harvested by individual plant0
For seed；
(5) strain single-strain planting DH is pressed1Generation, outdoor identification selection have to planthopper and striped rice borer walk quickly and keep away type insect resistace and
Zn content exceedes the strain that 30mg/kg 2 characters polymerize；
(6) strain single-strain planting DH is pressed2Generation, indoor identification, with reference to outdoor great Qu contrast identification selection to planthopper and two
Changing snout moth's larva has the strain for walking quickly and keeping away the 2 characters polymerization of type insect resistace and Zn content more than 30mg/kg；
(7) DH is planted by strain3In generation, by ripe phase seed selection, selection breeding time is no more than 135 days ripe phases moderate excellent product
System；
(8) DH is planted by strain4In generation, obtain and walk quickly and keep away the pest-resistant and high zinc paddy rice of type and moderate sizing strain of ripe phase, that is, become
Keep away the pest-resistant and high zinc rice varieties of type.
It is Zhejiang University's seed selection pair preferably, walking quickly and keeping away type insect-proof rice parent I described in step (1) for the anti-rice in Zhejiang No. 1
Planthopper and striped rice borer, which have, walks quickly and keeps away type insect resistace green and the special long-grained nonglutinous rice new varieties of organic rice.The kind is to Ministry of Agriculture plant
The application of New variety protection office accepts New variety protection, application number：20130457.9, agricultural college of Zhejiang University can be purchased from.Should
Rice Varieties plant hopper and striped rice borer, which have, walks quickly and keeps away type insect resistace, Zn content only 11.6mg/kg, is the 1/ of II grains tastes of parent
2.8, it is common low zinc content kind.
Preferably, the parental rice II described in step (1) is grown for grain grain.It is mini that Li Lizishi Zhejiang University cultivates
High zinc content japonica rice variety, is referred to：Zhang Linlin, Han Juanying, Liu Zhen, Shu little Li, the choosing of the mini high zinc content paddy rice of Wu hall star
Educate and its Chinese rice of feature, 2011,17 (6)：66-68.The rice varieties are Zn content height in high zinc content, polished rice
It is typical high zinc content up to 32.8mg/kg, quality is excellent, height sense worm, to planthopper and striped rice borer without walking quickly and keeping away type insect resistace.
Preferably, grow hybridization combo and through Anther Culture from the anti-rice in Zhejiang No. 1 and grain grain, seed selection walk quickly and keep away type it is pest-resistant and
The rice varieties of high zinc character polymerization, have to planthopper and striped rice borer and walk quickly and keep away type insect resistace, Zn content 31.2mg/kg is Zhejiang
2.7 times of anti-rice No. 1 (11.6mg/kg), are typical high zinc content kind.
Compared with prior art, beneficial effects of the present invention are：
(1) rice varieties that the present invention is bred as, 2 kinds of good characteristics with the pest-resistant and high zinc of type of walking quickly and keeping away are taken into account, Ke Yiyou
Effect supplement zinc nutrition promotes growing for baby, and green can ensure foodsafety with organic production；
(2) in view of indica type Anther Culture be difficult, round-grained rice type Anther Culture easily, take and be returned again to enter for 2 times with round-grained rice type parent II
Row Anther Culture, walks quickly and keeps away the pest-resistant and high zinc of type to set up big mass screening and lays a good foundation, compared with hybridization technique, Anther Culture
Technology can fast and stable with realize it is early judge to identify, breeding cycle is short.
Embodiment
The invention will be further described with reference to embodiments.The present embodiment is under premised on technical solution of the present invention
Implemented, give detailed embodiment and process, but protection scope of the present invention includes but is not limited to following implementations
Example.
1. breeding material：
(1) it is female parent with the anti-rice in Zhejiang No. 1.The anti-rice in Zhejiang No. 1, which is Zhejiang University's seed selection, to be had to planthopper and striped rice borer and walks quickly and keeps away
Type insect resistace green and the special long-grained nonglutinous rice new varieties of organic rice.The kind to Plant new variety protection office of the Ministry of Agriculture application by
Manage New variety protection, application number：20130457.9, agricultural college of Zhejiang University can be purchased from.The Rice Varieties plant hopper and two changes
Snout moth's larva, which has, walks quickly and keeps away type insect resistace, Zn content only 11.6mg/kg, is the 1/2.8 of II grains tastes of parent, is common low zinc content product
Kind.
(2) grown with grain grain for male parent.The mini high zinc content japonica rice variety that Li Lizishi Zhejiang University cultivates, is referred to：
The Chinese rice of Zhang Linlin, Han Juanying, Liu Zhen, Shu little Li, the seed selection of the mini high zinc content paddy rice of Wu hall star and its feature
Rice, 2011,17 (6)：66-68., it is commercially available or give in agricultural college of Zhejiang University.The rice varieties are high zinc, and content is up to
32.8mg/kg, is typical high zinc content, and quality is excellent, height sense worm, to planthopper and striped rice borer without walking quickly and keeping away type insect resistace.
2. the Breeding Process for walking quickly and keeping away the pest-resistant and high zinc rice varieties of type is：
(1) 2011 year spring and summer, Zhejiang Hangzhou is female parent to walk quickly and keep away the pest-resistant parent I of type (the anti-rice in Zhejiang No. 1), with Gao Xinhan
It is male parent to measure parent II (grain grain is grown), hybridizes to obtain F1For 5, seed；
(2) 2012 years winter-spring seasons, F is planted in Hainan Lingshui15 plants of plant, with high zinc content parent II (grain grain is grown) for male parent
Backcrossing 1 time, obtains BC1F112, seed；
(3) 2012 year spring and summer, Zhejiang Hangzhou plants BC1F1For 12 plants of plant, with high zinc content parent II (grain grain is grown)
It is returned again for male parent 1 time, obtains BC as much as possible2F198, seed；
(3) 2013 years winter-spring seasons, BC is planted in Hainan Lingshui2F1In 98 plants of generation, built using anther culture technique and double single times
Body (DH) colony；
(4) 2013 year summer, DH is harvested by individual plant0For seed, 1096, DH systems are built；
(5) 2014 years winter-spring seasons, Hainan Lingshui, by single-strain planting DH1System, is harvested by individual plant, first outdoor identification selection pair
Planthopper and striped rice borer have walk quickly and keep away type insect resistace 132 plants, then detect that Zn content exceedes 30mg/kg 21 plants of plant；
(6) 2014 year summer and autumn, Zhejiang Hangzhou plants DH by strain2Generation, indoor identification, with reference to outdoor great Qu contrast reflect
Selection has 13 plants that walk quickly and keep away type insect resistace to planthopper and striped rice borer calmly, detects that Zn content is stable again and exceedes 30mg/kg's
Excellent strain 11；
(7) 2015 year summer and autumn, Zhejiang Hangzhou plants DH by strain3In generation, by ripe phase seed selection, selection breeding time is no more than
135 days ripe phases moderate excellent strain 2；
(8) 2016 year summer and autumn, Zhejiang Hangzhou plants DH by strain4The pest-resistant and high a variety of nutrition of zinc of type are walked quickly and kept away in generation, acquisition
The stable sizing strain 1 for taking into account the ripe phase of character, that is, walk quickly and keep away the pest-resistant and high zinc rice varieties of type.
The pest-resistant and high zinc rice varieties of type are walked quickly and kept away in the present embodiment seed selection：It is pest-resistant with type is walked quickly and kept away to planthopper and striped rice borer
Property, Zn content 31.2mg/kg is 2.7 times of the anti-rice in Zhejiang No. 1 (11.6mg/kg), is typical high zinc content kind.And parent I
The anti-rice in Zhejiang No. 1 has to planthopper and striped rice borer walks quickly and keeps away type insect resistace, Zn content only 11.6mg/kg, is that II grains of parent are grown
1/2.8, it is common low zinc content kind；It is high zinc that II grains of parent, which are grown, and content is up to 32.8mg/kg, and quality is excellent, height sense worm,
To planthopper and striped rice borer without walking quickly and keeping away type insect resistace.
Technical scheme and beneficial effect are described in detail embodiment described above, it should be understood that
The specific embodiment of the present invention is the foregoing is only, is not intended to limit the invention, it is all to be done in the spirit of the present invention
Any modification, supplement and equivalent substitution etc., should be included in the scope of the protection.
Claims (3)
1. a kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type, it is characterised in that including：
(1) selection walks quickly and keeps away type insect-proof rice kind for parent I, and high zinc content paddy rice is parent II, is hybridized, and obtains F1Seed；
(2) F is planted1In generation, it is returned 1 time by male parent of parent II, obtains BC1F1For seed；
(3) BC is planted1F1In generation, it is returned again as male parent 1 time using parent II, obtains BC2F1Seed；
(4) BC is planted2F1Plant, doubled haploid (DH) colony is built using anther culture technique, and DH is harvested by individual plant0In generation, plants
Son；
(5) strain single-strain planting DH is pressed1In generation, outdoor identification selection has to planthopper and striped rice borer walks quickly and keeps away type insect resistace and zinc contains
The strain that 2 characters of the amount more than 30mg/kg polymerize；
(6) strain single-strain planting DH is pressed2In generation, indoor identification, have to planthopper and striped rice borer with reference to outdoor great Qu contrast identification selections
Have and walk quickly and keep away the strain that 2 characters of type insect resistace and Zn content more than 30mg/kg polymerize；
(7) DH is planted by strain3In generation, by ripe phase seed selection, selection breeding time is no more than 135 days ripe phases moderate excellent strain；
(8) DH is planted by strain4In generation, obtain and walk quickly and keep away the pest-resistant and high zinc paddy rice of type and moderate sizing strain of ripe phase, that is, walk quickly and keep away type anti-
Worm and high zinc rice varieties.
2. the selection according to claim 1 for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type, it is characterised in that step (1) institute
That states walks quickly and keeps away type insect-proof rice parent I for the anti-rice in Zhejiang No. 1.
3. the selection according to claim 1 for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type, it is characterised in that step (1) institute
The parental rice II stated is that grain grain is grown.
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| CN201710251284.5A CN106962177A (en) | 2017-04-14 | 2017-04-14 | A kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type |
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| CN201710251284.5A CN106962177A (en) | 2017-04-14 | 2017-04-14 | A kind of selection for walking quickly and keeping away the pest-resistant and high zinc paddy rice of type |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783407A (en) * | 2012-08-15 | 2012-11-21 | 江苏丰源种业有限公司 | Breeding method of high-rice-blast-resistance rice intermediate materials |
| CN103891607A (en) * | 2014-04-15 | 2014-07-02 | 浙江杰农生物科技有限公司 | Method for breeding sterile line of high-zinc rice |
-
2017
- 2017-04-14 CN CN201710251284.5A patent/CN106962177A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783407A (en) * | 2012-08-15 | 2012-11-21 | 江苏丰源种业有限公司 | Breeding method of high-rice-blast-resistance rice intermediate materials |
| CN103891607A (en) * | 2014-04-15 | 2014-07-02 | 浙江杰农生物科技有限公司 | Method for breeding sterile line of high-zinc rice |
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Application publication date: 20170721 |