CN106950263B - A kind of multi-functional electrochemical sensor and preparation method thereof - Google Patents
A kind of multi-functional electrochemical sensor and preparation method thereof Download PDFInfo
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- CN106950263B CN106950263B CN201710172819.XA CN201710172819A CN106950263B CN 106950263 B CN106950263 B CN 106950263B CN 201710172819 A CN201710172819 A CN 201710172819A CN 106950263 B CN106950263 B CN 106950263B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/46104—Devices therefor; Their operating or servicing
- C02F1/46109—Electrodes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
Abstract
The invention belongs to electrochemical fields, and in particular to one kind is captured simultaneously, is sterilized, multi-functional electrochemical sensor of detection and preparation method thereof.The sensor, which is negative, is loaded with the ZnO nanorod of AgNPs and vancomycin；Wherein, the mass ratio 1:1-1:6 of AgNPs and vancomycin.It is preparation method simple process of the invention, easily controllable, low in cost, it is prepared for capturing simultaneously, sterilize, the multi-functional electrochemical sensor of detection.Efficient killing effect can be played to the harmful microorganism in water body using the synergistic effect of vancomycin and nano silver, at the same can germ in separation detection water body, will be had a good application prospect in fields such as water body purifications.
Description
Technical field
The invention belongs to electrochemical fields, and in particular to one kind is captured simultaneously, is sterilized, the multi-functional electrochemical sensing of detection
Device and preparation method thereof.
Background technique
It is annual that there are many people to die of bacterium water pollution, such as " superbacteria " pollution.If germ contamination can be by very
It is fast to be diagnosed and bacterium is killed by quick remove, then the death rate of bacterium infection will substantially reduce [1].
Electrochemica biological sensor is a kind of analysis method established based on solid electrode and bio-sensing molecule.Because electric
Chemical sensor has, efficiently, simply, it is easily operated at low cost the advantages that electrochemical sensor, be widely used at present
In analysis DNA [2] and viral [3] etc..And in numerous Bacteria Detection technologies, electrochemica biological sensor causes widely
Concern, for example, Mireia et al. [4] reports a kind of electrochemical sensor detection pathogenic bacteria based on polypeptide identification bacterium.But
It is that current electrochemical sensor can only carry out simple detection bacterium, cannot has while remove, detects, more function of sterilization
Energy.Therefore, it is badly in need of developing a kind of while captures, sterilizes, the multi-functional electrochemica biological sensor of detection.
[1]M.E.Brecher,S.N.Hay,Bacterial contamination of blood components.
[J].Clinical Microbiology Reviews 2005,18(1),195-204.
[2]X。Li,J.Guo,Q.Zhai,J.Xia,G.Yi.[J].Ultrasensitive electrochemical
biosensor for specific detection of DNA based on molecular beacon mediated
circular strand displacement polymerization and hyperbranched rolling circle
amplification.[J].Analytica Chimica Acta 934(2016)52-58
[3]K.Shang,X.Wang,B.Sun,Z Cheng,S.Ai,β-cyclodextrin-ferrocene host–
guest complex multifunctional labeling triple amplification strategy for
electrochemical immunoassay of subgroup J of avian leukosis viruses.[J]
.Biosensors and Bioelectronics 45(2013)40-45.
[4]H.N Mireia,B.O.Sergi,N.Abramova,F.X.A.Bratov,M.M.Carlos,
F.J.Gil.Impedimetric antimicrobial peptide-based sensor for the early
detection of periodontopathogenic bacteria.[J].Biosensors and Bioelectronics
86(2016)377–385.
Summary of the invention
It is an object of the invention to aiming at the problems existing in the prior art, provide a kind of while capturing, sterilize, detection
Multi-functional electrochemical sensor and preparation method thereof.
To achieve the above object, the present invention is implemented using following technical scheme:
A kind of multi-functional electrochemical sensor, the sensor, which is negative, is loaded with AgNPs (having a size of 5~200nm) and through the ages
The ZnO nanorod of mycin；Wherein, the mass ratio of AgNPs and vancomycin is 1:1-1:6.
The AgNPs is as sterili-sation unit, and vancomycin is as recognition unit.
A kind of preparation method of multi-functional electrochemical sensor described in claim 1:
(1) preparation of ZnO nanorod: the FTO after cleaning is covered with aluminium flake, is later immersed in the FTO that aluminium flake covers
By Zn (NO3)2·6H2In O and hexa solution, in 60-90 DEG C of 1~4h of water-bath, for use；
(2) above-mentioned acquisition ZnO nanorod the preparation of AgNPs/ZnO/FTO: is immersed in AgNO3In solution；Then utilize
Cyclic voltammetry was impregnating AgNO3ZnO nanorod on reduction generate nano silver；
(3) preparation of Van/AgNPs/ZnO/FTO Multifunction Sensor: will be obtained in step (2) AgNPs/ZnO/FTO according to
It is secondary to be immersed in mercaptoacetic acid solution, 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC)/N- hydroxyl respectively
In succinimide (NHS) and vancomycin solution；The EDC/NHS mole ratio is 2/1；Wherein, the end of EDC and NHS is dense
Degree is 5~50mM.
The mass ratio of the AgNPs and vancomycin is 1:1~1:6.
FTO after the cleaning is that FTO is successively used to acetone respectively, the NaOH mixed solution of ethanol/water, ultrapure water ultrasound
Clean 10~15min；Wherein, the NaOH of ethanol/water mixes molten middle NaOH concentration as 1-2M, and the ratio of ethyl alcohol and water is v/v=
1:1。
Solution is cooled to room temperature after water-bath in the step (1), is rinsed through distilled water, in 30~40 DEG C of dryings after flushing
After 2~10h, the FTO of the ZnO modification with column structure, i.e. ZnO nanorod are obtained.
Zn (NO in the step (1)3)2·6H2O is Zn (NO3)2·6H2O will be added in ultrapure water magnetic agitation to complete
Whole soln；Hexa solution is that hexa is added in ultrapure water to magnetic agitation to perfect solution；Zn
(NO3)2·6H2O and hexa solution are by isometric mixing.
AgNO was impregnated described in the step (2)3ZnO nanorod under voltage -0.8~0 cyclic voltammetry scan,
Sweep speed 10mV/s scans 10 sections of number of segment；It is cleaned using distilled water, be can be obtained after last 30~40 DEG C dry 2~10h later
AgNPs@ZnO/FTO。
AgNPs/ZnO/FTO impregnates 2~12h in mercaptoacetic acid solution in the step (3), is 1- (3- diformazan ammonia
Base propyl) 2~5h is impregnated in -3- ethyl-carbodiimide hydrochloride (EDC)/n-hydroxysuccinimide (NHS), finally in containing
2~5h in the vancomycin aqueous solution of 3mg.
A kind of application of Van/AgNPs/ZnO/FTO Multifunction Sensor, the Van/AgNPs/ZnO/FTO electrode conduct
Electrochemical sensor is in detection bacterium or as the application in fungicide.
The AgNPs@ZnO/FTO Multifunction Sensor of the vancomycin functionalization removes the application of solution bacterium.
Application of the AgNPs@ZnO/FTO of the vancomycin functionalization in water body Bacteria Detection.
Bacterium in the water body is leather orchid Yang Shi bacterium, such as staphylococcus aureus, staphylococcus albus etc..
The AgNPs@ZnO/FTO Multifunction Sensor of vancomycin functionalization is applied in water body, below only to nocuousness
It for the detection of microorganism staphylococcus glucose coccus (Staphylococcus aureus), removes, kills, using CHI
The detection of 760C electrochemical workstation；
It is detected method particularly includes: is used CHI 760C electrochemical workstation, is to electrode, to be saturated calomel electricity with Pt piece
Pole is as reference electrode, using the Multifunction Sensor of preparation as working electrode；Different Multifunction Sensors are immersed in a series of
10~30min in the microbial solution of concentration, later detection liquid in row electrochemical measurement.The bacterial concentration is 102~
106cfu/mL；The detection liquid is 5mM [Fe (CN)6]3-/4-Solution, pH value 7.4.
It is removed method particularly includes: the sensor of preparation is immersed in 10~30min in bacterial solution, later leads to solution
Colony counting method measurement remaining bacteria concentration is crossed, clearance rate is calculated.The bacterial concentration is 500cfu/mL.
Its bactericidal activity method particularly includes: sensor will be prepared and be immersed in 2~12h in bacterial solution, pass through plate count
Method measures surviving bacteria concentration, calculates killing rate.The bacterial concentration is 106cfu/mL。
The beneficial effects of the present invention are:
The present invention combines AgNPs and vancomycin, and nano silver (AgNPs) can be by discharging Ag+Destroy bacterium
Cell membrane causes cell death, therefore AgNPs is with efficient antibacterial effect and it is with hypotoxicity.Meanwhile vancomycin is made
For a kind of antibiotic, there is bactericidal effect to various bacteria, while its amino for having and carboxylic group can be with bacterial cells
Peptide glycan on wall forms hydrogen bond, thus therefore playing the role of capture bacterium combines energy for vancomycin and AgNPs
Enough play the role of Synergistic biocidal, be greatly improved its bactericidal effect, will test, clear, the function of sterilization combines, can be with
The application range of extension sensor, it is significant to exploitation novel sensor；It is specific:
(1) present invention has obtained vancomycin functionalization by combining vancomycin with AgNPs@ZnO/FTO
AgNPs@ZnO/FTO Multifunction Sensor, preparation method simple process are easily controllable, low in cost；
(2) the AgNPs@ZnO/FTO sensor of vancomycin functionalization prepared by the present invention has detection, removes, sterilization
It is multi-functional；
(3) the AgNPs@ZnO/FTO sensor of vancomycin functionalization prepared by the present invention can be detected quickly in water body
Bacterial content, detection be limited to 100cfu/mL；
(4) the AgNPs@ZnO/FTO sensor of vancomycin functionalization prepared by the present invention can be removed quickly in water body
Bacterium, clearance rate is 40% in 1h；
(5) the AgNPs@ZnO/FTO sensor of vancomycin functionalization prepared by the present invention has efficient sterilizing effect, right
Concentration is 106Killing rate can reach 99.99% in the microorganism 12h of cfu/mL
(6) the AgNPs@ZnO/FTO sensor of vancomycin functionalization prepared by the present invention it is with good stability and
Reusing has good application prospect in the fields such as water body purification and medical diagnosis.
Detailed description of the invention
Fig. 1 is that the SEM of sample provided in an embodiment of the present invention schemes, wherein (A) ZnO/FTO, (B) AgNPs@ZnO/FTO；
Fig. 2 is examination criteria curve graph of the Multifunction Sensor provided in an embodiment of the present invention to the bacterium of various concentration；
Fig. 3 is sterilizing rate (A) and separative efficiency of the sample provided in an embodiment of the present invention to staphylococcus aureus
(B), in which: ordinate is Antibacterial rate (sterilizing rate), unit % in A figure；Abscissa is that capture is thin in B figure
Bacterium amount, ordinate are separative efficiency, unit %.
Specific embodiment
Below by way of specific embodiment, the invention will be further described, facilitates those skilled in the art more
It is fully understood by the present invention, but do not limit the invention in any way.
The invention belongs to electrochemical fields, and in particular to one kind is captured simultaneously, is sterilized, the multi-functional electrochemical sensing of detection
Device and preparation method thereof.The sensor is by vancomycin, nano silver, ZnO nanorod composition.It is prepared first in FTO electrode table
Wheat flour is for 3D column ZnO material, then using electrochemical reduction method in ZnO surface finish nano silver, then by thioacetic acid and through the ages
Vancomycin is accessed electrode surface by mycin reaction.There is efficient killing effect to harmful microorganism in water body, it is net in water body
The fields such as change will have a good application prospect.
Embodiment 1:
The preparation method of the AgNPs@ZnO/FTO Multifunction Sensor of vancomycin functionalization:
(1) preparation of ZnO/FTO: by 50mM Zn (NO3)2·6H2O is added in ultrapure water, magnetic agitation to being completely dissolved,
It is named as solution A；50mM hexa, which is added to magnetic agitation in ultrapure water, is named as solution to perfect solution simultaneously
B；Then solution A and solution B are mixed in equal volume.The FTO after the cleaning of aluminium flake package is immersed in above-mentioned mixed solution later
In, conducting surface is placed vertically downward, is put into 90 DEG C of water-baths and is reacted 3h；Then solution is cooled to room temperature, FTO is taken out, is passed through
Rinse the FTO (such as Figure 1A) with the ZnO modification that after 40 DEG C of dry 6h, can obtain that there is column structure；
FTO after the cleaning is that FTO is successively used to acetone respectively, the NaOH mixed solution of ethanol/water, ultrapure water ultrasound
Clean 10~15min；Wherein, the NaOH of ethanol/water mixes molten middle NaOH concentration as 1M, and the ratio of ethyl alcohol and water is v/v=1:
1。
(2) preparation of AgNPs@ZnO/FTO: ZnO/FTO obtained in step (1) is immersed in containing 10mL 0.25mM
AgNO3Solution deposits nano silver by cyclic voltammetry, is cleaned later using distilled water, can be obtained after last 40 DEG C dry 6h
AgNPs@ZnO/FTO (referring to Figure 1B)；
It is described to impregnate AgNO3ZnO nanorod under voltage -0.8~0 cyclic voltammetry scan, sweep speed 10mV/
S scans 10 sections of number of segment.
(3) preparation of the AgNPs@ZnO/FTO of vancomycin functionalization: by AgNPs@ZnO/FTO obtained in step (2)
It is immersed in 2h in mercaptoacetic acid solution, 2h in 50mM EDC/NHS solution is dipped in after flushing.Be dipped in again containing
2h in the vancomycin solution of 3mg.Obtained electrode is Van/AgNPs@ZnO/FTO.
The mercaptoacetic acid solution concentration is 5mM；The final concentration of 50mM of EDC and NHS, wherein EDC/NHS is between the two
Dosage 2:1.
Embodiment 2: above-mentioned gained Multifunction Sensor is applied in water body, to harmful microorganism staphylococcus aureus
Detection performance.
With staphylococcus aureus (500~106) evaluation vancomycin functionalization AgNPs@ZnO/FTO multifunctional sensing
Device:
Prepare bacterial suspension first, staphylococcus aureus storing liquid is inoculated into sterilizing LB liquid medium, then
It places it in 37 DEG C, in the air constant-temperature table of 150rpm, is incubated overnight.It is suspended in after cultivating obtained bacterial suspension centrifugation
In 0.01mol/L PBS (pH=7.4) buffer, obtaining concentration is 1 × 108The staphylococcus aureus suspension of cfu/mL, to
With
Above-mentioned bacterial suspension is diluted to 100 with sterilizing 0.01mol/L PBS (pH=7.4) buffer in test experience~
106Multifunction Sensor prepared by the present invention is immersed in 1h in the bacterium solution of various concentration by cfu/mL concentration gradient respectively later.
It is immersed in after Multifunction Sensor is cleaned later in 5mM potassium ferricyanide solution and carries out ac impedance measurement.Every group of reality in experiment
It tests and is both needed to be measured in parallel 3 times, be averaged as final result (referring to fig. 2).
The variation of impedance value and lg C are linear as seen from Figure 2, equation of linear regression are as follows: y=724.5lg
C -1342.42 (R=0.9987)
Embodiment 3:
The Ag@ZnO/FTO Multifunction Sensor of above-mentioned gained vancomycin functionalization is golden yellow to harmful microorganism in water body
The staphylococcic killing of color and centrifugation.
Sterilization experiment:
Staphylococcus aureus liquid is taken to be diluted to 10 with sterilizing 0.01mol/L PBS (pH=7.4) buffer6cfu/
mL.Bacterium solution after dilution is placed in 5mL reactor, is added and immerses the Multifunction Sensor prepared in embodiment 1 in bacterium solution,
1.0mL reaction solution is taken after reaction 12h, is successively diluted with 0.01mol/L PBS (pH=7.4) buffer according to serial dilutions several
A gradient (10,100,100 times) then takes 100 μ L to the LB having had been prepared for solid respectively from the solution of different extension rates
On body culture medium, bacterium solution is equably applied on LB culture medium.LB culture medium is inverted, is put into 37 in electro-heating standing-temperature cultivator
For 24 hours, by counting the bacterium colony number that grows on culture medium, and corresponding extension rate obtains bacterial concentration, with determination for DEG C culture
The survival rate and sterilizing rate of bacterium.Every group of experiment is both needed to be measured in parallel 3 times in experiment, is averaged as final result, blank
Experiment is as a control group (referring to Fig. 3 A).
By Fig. 3 (A) it can be seen that prepared Van/Ag@ZnO/FTO in sterilizing rate is for 24 hours 99.99%, much higher than sky
The sterilizing rate of white control group and ZnO/FTO.Bacterium separating experiment:
Staphylococcus aureus liquid is taken to be diluted to 500 Hes respectively with sterilizing 0.01mol/L PBS (pH=7.4) buffer
1000cfu/mL is put into later in 1mL reactor, and the Multifunction Sensor of preparation is immersed in bacterium solution, will can by Fig. 3 (B)
See, the Multifunction Sensor of preparation shows good bacterium centrifugation.
Claims (9)
1. a kind of multi-functional electrochemical sensor, it is characterised in that: sensor is the AgNPs@ZnO/ of vancomycin functionalization
FTO；Wherein, the mass ratio 1:1-1:6 of AgNPs and vancomycin；
The preparation method of the multi-functional electrochemical sensor: (1) preparation of ZnO/FTO: the FTO after cleaning is covered with aluminium flake
The FTO that aluminium flake covers is immersed in Zn (NO later by lid3)2·6H2It is anti-in 60-90 DEG C of water-bath in O and hexa solution
1~4h is answered, for use；
(2) above-mentioned acquisition ZnO/FTO the preparation of AgNPs@ZnO/FTO: is immersed in AgNO3In solution；Then utilize cyclic voltammetric
Method was impregnating AgNO3ZnO/FTO on reduction generate nano silver；
(3) AgNPs@ZnO/FTO vancomycin/AgNPs@ZnO/FTO Multifunction Sensor preparation: will be obtained in step (2)
It is successively immersed in mercaptoacetic acid solution, 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC)/N- hydroxyl respectively
In base succinimide (NHS) and vancomycin solution, wherein EDC/NHS mole ratio is 2/1；
The mass ratio of the AgNPs and vancomycin is 1:1-1:6.
2. a kind of preparation method of multi-functional electrochemical sensor described in claim 1, it is characterised in that: (1) ZnO/FTO
Preparation: the FTO after cleaning is covered with aluminium flake, and the FTO that aluminium flake covers is immersed in Zn (NO later3)2·6H2O and hexa-methylene
In four amine aqueous solutions, in 60-90 DEG C of 1~4h of water-bath, for use；
(2) above-mentioned acquisition ZnO/FTO the preparation of AgNPs@ZnO/FTO: is immersed in AgNO3In solution；Then utilize cyclic voltammetric
Method was impregnating AgNO3ZnO/FTO on reduction generate nano silver；
(3) AgNPs@ZnO/FTO vancomycin/AgNPs@ZnO/FTO Multifunction Sensor preparation: will be obtained in step (2)
It is successively immersed in mercaptoacetic acid solution, 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC)/N- hydroxyl respectively
In base succinimide (NHS) and vancomycin solution, wherein EDC/NHS mole ratio is 2/1；
The mass ratio of the AgNPs and vancomycin is 1:1-1:6.
3. the preparation method of multi-functional electrochemical sensor as described in claim 2, it is characterised in that: after the cleaning
FTO is that FTO is successively used to acetone respectively, and the ethanol water containing NaOH, ultrapure water is cleaned by ultrasonic 10~15min；Wherein, contain
Having in the ethanol water of NaOH NaOH concentration is 1-2M, and the ratio of ethyl alcohol and water is v/v=1:1.
4. the preparation method of multi-functional electrochemical sensor as described in claim 2, it is characterised in that: in the step (1)
Solution is cooled to room temperature after water-bath, is rinsed through distilled water, after flushing after 30~40 DEG C of dry 2~10h, is obtained with column
The FTO, i.e. ZnO/FTO of the ZnO modification of structure.
5. the preparation method of multi-functional electrochemical sensor according to claim 3, it is characterised in that: in the step (1)
Zn(NO3)2·6H2O and hexa solution mix in equal volume.
6. the preparation method of multi-functional electrochemical sensor according to claim 3, it is characterised in that: in the step (2)
It is described to impregnate AgNO3ZnO/FTO under voltage -0.8~0 cyclic voltammetry scan, sweep speed 10mV/s, scan number of segment
10 sections；It is cleaned later using distilled water, AgNPs@ZnO/FTO can be obtained after last 30~40 DEG C dry 2~10h.
7. the preparation method of multi-functional electrochemical sensor according to claim 3, it is characterised in that: in the step (3)
AgNPs@ZnO/FTO impregnates 2~12h in mercaptoacetic acid solution, in 1- (3- dimethylamino-propyl) -3- ethyl carbodiimide salt
2~5h is impregnated in hydrochlorate (EDC)/n-hydroxysuccinimide (NHS), finally 2 in the vancomycin aqueous solution containing 3mg~
5h。
8. a kind of application of multi-functional electrochemical sensor described in claim 1, it is characterised in that: the vancomycin function
Application of the AgNPs@ZnO/FTO electrode of change as electrochemical sensor in detection bacterium.
9. a kind of application of multi-functional electrochemical sensor described in claim 1, it is characterised in that: the vancomycin function
The AgNPs@ZnO/FTO electrode of change is as electrochemical sensor as the application in fungicide.
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| CN104198558A (en) * | 2014-09-05 | 2014-12-10 | 天津工业大学 | Preparation method of novel escherichia coli electrochemical sensor |
| CN104697977A (en) * | 2015-03-23 | 2015-06-10 | 苏州大学 | Silicon-based SERS multifunctional chip and preparation method thereof |
| CN106501337A (en) * | 2016-11-29 | 2017-03-15 | 吉林大学 | A kind of electrochemical detection method of staphylococcus aureuses |
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