CN106943310B - Compound for improving light stability of chromoprotein and application - Google Patents
Compound for improving light stability of chromoprotein and application Download PDFInfo
- Publication number
- CN106943310B CN106943310B CN201710060788.9A CN201710060788A CN106943310B CN 106943310 B CN106943310 B CN 106943310B CN 201710060788 A CN201710060788 A CN 201710060788A CN 106943310 B CN106943310 B CN 106943310B
- Authority
- CN
- China
- Prior art keywords
- vitamin
- phycocyanin
- amino acid
- chromoprotein
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 150000001875 compounds Chemical class 0.000 title claims abstract description 27
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 92
- 239000011718 vitamin C Substances 0.000 claims abstract description 54
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 45
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 45
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 45
- 239000000203 mixture Substances 0.000 claims abstract description 40
- 150000001413 amino acids Chemical class 0.000 claims abstract description 35
- 239000000049 pigment Substances 0.000 claims abstract description 34
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 30
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 30
- 235000013305 food Nutrition 0.000 claims abstract description 26
- 239000002537 cosmetic Substances 0.000 claims abstract description 24
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims abstract description 8
- 150000003700 vitamin C derivatives Chemical class 0.000 claims abstract description 6
- 150000005846 sugar alcohols Chemical class 0.000 claims abstract description 5
- 108010053210 Phycocyanin Proteins 0.000 claims description 85
- 108010004729 Phycoerythrin Proteins 0.000 claims description 43
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 36
- 235000001014 amino acid Nutrition 0.000 claims description 26
- 239000004471 Glycine Substances 0.000 claims description 18
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 abstract description 27
- 230000000694 effects Effects 0.000 abstract description 17
- 238000000034 method Methods 0.000 abstract description 16
- 239000000463 material Substances 0.000 abstract description 4
- 230000007935 neutral effect Effects 0.000 abstract description 4
- 230000002411 adverse Effects 0.000 abstract description 3
- 230000003711 photoprotective effect Effects 0.000 abstract description 3
- 230000002378 acidificating effect Effects 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 150000003862 amino acid derivatives Chemical class 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 41
- 239000008367 deionised water Substances 0.000 description 30
- 229910021641 deionized water Inorganic materials 0.000 description 30
- 230000032683 aging Effects 0.000 description 29
- 229910052724 xenon Inorganic materials 0.000 description 29
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 29
- 238000002360 preparation method Methods 0.000 description 21
- 238000005303 weighing Methods 0.000 description 19
- 238000003756 stirring Methods 0.000 description 17
- 239000007864 aqueous solution Substances 0.000 description 16
- 239000000843 powder Substances 0.000 description 16
- 239000000523 sample Substances 0.000 description 15
- 239000000243 solution Substances 0.000 description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 11
- 235000018417 cysteine Nutrition 0.000 description 11
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 11
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 238000002156 mixing Methods 0.000 description 8
- 229940088594 vitamin Drugs 0.000 description 8
- 229930003231 vitamin Natural products 0.000 description 8
- 235000013343 vitamin Nutrition 0.000 description 8
- 239000011782 vitamin Substances 0.000 description 8
- 150000003722 vitamin derivatives Chemical class 0.000 description 8
- 235000016425 Arthrospira platensis Nutrition 0.000 description 7
- 240000002900 Arthrospira platensis Species 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 7
- 239000004386 Erythritol Substances 0.000 description 6
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 6
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 6
- 229940009714 erythritol Drugs 0.000 description 6
- 235000019414 erythritol Nutrition 0.000 description 6
- 238000007710 freezing Methods 0.000 description 6
- 230000008014 freezing Effects 0.000 description 6
- 239000007938 effervescent tablet Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 235000010378 sodium ascorbate Nutrition 0.000 description 5
- 229960005055 sodium ascorbate Drugs 0.000 description 5
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 5
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 5
- 229940082787 spirulina Drugs 0.000 description 5
- 238000009472 formulation Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000004475 Arginine Substances 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
- 102000002067 Protein Subunits Human genes 0.000 description 3
- 108010001267 Protein Subunits Proteins 0.000 description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 3
- 235000010376 calcium ascorbate Nutrition 0.000 description 3
- 229940047036 calcium ascorbate Drugs 0.000 description 3
- 239000011692 calcium ascorbate Substances 0.000 description 3
- BLORRZQTHNGFTI-ZZMNMWMASA-L calcium-L-ascorbate Chemical compound [Ca+2].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] BLORRZQTHNGFTI-ZZMNMWMASA-L 0.000 description 3
- 239000013068 control sample Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 235000013922 glutamic acid Nutrition 0.000 description 3
- 239000004220 glutamic acid Substances 0.000 description 3
- 239000006210 lotion Substances 0.000 description 3
- 239000002932 luster Substances 0.000 description 3
- 238000000859 sublimation Methods 0.000 description 3
- 230000008022 sublimation Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- 241000168525 Haematococcus Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 229940082509 xanthan gum Drugs 0.000 description 2
- 235000010493 xanthan gum Nutrition 0.000 description 2
- 239000000230 xanthan gum Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000042238 Eurycoma Species 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- 241000206613 Pyropia yezoensis Species 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- -1 ascorbic acid salt Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000012736 patent blue V Nutrition 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052594 sapphire Inorganic materials 0.000 description 1
- 239000010980 sapphire Substances 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical group 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000003724 spirulina extract Nutrition 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
- A61K8/447—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof containing sulfur
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/42—Colour properties
- A61K2800/43—Pigments; Dyes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a compound for improving the light stability of chromoprotein and application thereof, wherein the compound is a mixture containing amino acid and vitamin C; the amino acid is any one of acidic amino acid, neutral amino acid, basic amino acid and amino acid derivatives; the vitamin C is one or more of vitamin C and vitamin C salt; when the amino acid-vitamin C mixture is compounded with the pigment protein, sugar or sugar alcohol is added as an auxiliary material. The compound of the invention can eliminate the adverse effect of amino acid on the chromoprotein and provide the photoprotection of the chromoprotein which is obviously superior to that provided by single vitamin C, thereby helping the chromoprotein to maintain natural color and activity. In addition, the method does not need to pretreat the pigment protein, does not change the structure of the pigment protein, and has great practical significance for the application of the pigment protein in cosmetics and foods.
Description
Technical Field
The invention mainly relates to the technical field of cosmetics and foods, in particular to the technical field of improving the light stability of chromoprotein.
Background
The natural active pigment has good application prospect in the fields of cosmetics, foods, health products and the like due to the physiological activity, safety and natural color. However, natural active pigments generally face the problem of poor stability, especially a class of pigment proteins: that is, a pigment composed of a chromophoric group and a protein subunit, is less stable due to binding to a protein. Phycocyanin and phycoerythrin are two typical chromoproteins.
Phycocyanin is mainly present in blue algae, red algae and cryptophyceae, and is one of the photosynthetic light-capturing pigments of these algae, particularly, it is present in spirulina in an amount of 10% -20% (Japanese value, Panling. research on the physiological activity of phycocyanin of spirulina has been advanced [ J ] medical review, 2006,12(2): 111-. Phycocyanin has characteristic absorption at 620nm, can show beautiful sapphire blue at a higher concentration, and can be widely applied to the fields of food, cosmetics and the like as a natural pigment because of sky blue at a lower concentration.
Phycocyanin has various skin effects of reducing damage of active oxygen (ROS) to skin keratinocytes, relieving skin peroxidation stress (cosmetic I, Manca M L, calcium n-L atomic A, Maccioni AM, Fadda AM, Mancon M. phytocyanine-expressing leucocytes as carrier for skin deletion and oxidation stress, damage J Mater Sci Mater Meer Med. Apr; 27(4) 75. also can promote skin anti-inflammatory activity (Mancia M, Pend S J, L ed nN, Moreremia T, Sinico C, Sasod L, Faycya AM. phytochrome for skin keratinocytes, K-D-A-D-A-D-A-D-A-D-A-D-A-D-A, A-A, a K-A.
In conclusion, the phycocyanin has good efficacy and application prospect in the fields of cosmetics and foods. But due to the specific structure of phycocyanin: the tetrapyrrole chromophore binds to the protein subunit in a manner that results in poor stability, including thermostability and photostability. In particular, phycocyanin is easy to inactivate and fade under illumination (yoga, leaf ruin. spirulina platensis phycocyanin stability research [ J ]. food research and development, 2013,34(4):11-14), so that the color of phycocyanin is greatly limited, and the application of phycocyanin is also limited.
For example, European yoga et al found that 0.5 mol/L-2 mol/L of NaCl could improve phycocyanin stability (European yoga, leaf ruin. Spirulina platensis phycocyanin stability study [ J ] food research and development, 2013,34(4):11-14), but the increase in light stability was not significant.
Zhang Wa Fang et al found that the sugar solution can improve the heat stability of phycocyanin (Zhang Wa Fang, Liu Xuchuan, Li Qi Hua et al. Spirulina phycocyanin extraction and stability test [ J ]. Nature science version, 1999,21(3): 230-.
In addition, the royal soldier and the like try to wrap phycocyanin to improve the stability, and submit a patent application (application number: 201310243071.X), but the technology aims at that the phycocyanin of the spirulina is unstable, is easy to activate and denaturate, is oxidized and degenerates, influences the application of the phycocyanin of the spirulina, does not fundamentally solve the technical bottleneck of improving the light stability of the phycocyanin, and the wrapping color of the phycocyanin can be partially changed.
The Zhongshan university discloses a phycocyanin-vitamin C composite effervescent tablet and a preparation method thereof (CN201610601173.8), and points out that the stability of phycocyanin of the phycocyanin effervescent tablet added with vitamin C is superior to that of the phycocyanin effervescent tablet not added with vitamin C: the stability experiment for 20 days shows that the phycocyanin in the solution of the effervescent tablet added with the vitamin C is reduced by about 37 percent; but the phycocyanin in the solution is reduced by about 90 percent without adding vitamin C into the effervescent tablets; the patent improves the stability of phycocyanin in water to a certain extent.
The prior art also shows that vitamin C has an effect of improving the photostability of phycocyanin. However, the shelf life of food cosmetics is as long as 2-3 years, and the prior art shows that only about 10% of phycocyanin is left after 6-month shelf life simulation experiments of the effect of single vitamin C, which indicates that the single vitamin C cannot meet the requirement of cosmetics and food fields on the light stability of phycocyanin, and more stabilizing technologies and methods are yet to be developed.
Phycoerythrin is also a pigment protein with a tetrapyrrole chromophoric group binding protein subunit structure, has maximum absorption at about 560nm to show red color, is easy to fade, precipitate or discolor after illumination, and faces the difficulty of application similar to phycocyanin (Lucai, Europe, Haematococcus and Haematococcus phycoerythrin extraction and stability research [ J ]. Collection university report (Nature science edition), 2002,7(1): 15-19.). Therefore, it would be of great practical significance if a photoprotection technique could be developed for the chromoprotein.
Disclosure of Invention
Aiming at the current situation of the prior art, particularly poor light stability, and incapability of meeting the requirement of the fields of cosmetics and foods on the light stability of the pigment protein, the invention provides a compound for improving the light stability of the pigment protein, and the combination of an amino acid substance and vitamin C can obviously improve the light stability of the pigment protein. More importantly, when the amino acid substances are used independently, the light stability of the pigment protein is not improved, and even partial amino acid can accelerate the color fading of the pigment protein; but when the amino acid and the vitamin C are mixed for use, the light stability effect on the chromoprotein is obviously improved, the adverse effect of amino acid substances is eliminated, and the single effect of the vitamin C is more obviously superior. Mixture of amino acids and vitamin C can be used in food or cosmetic formulations containing chromoprotein for improving the photostability of chromoprotein.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention provides a compound for improving the light stability of chromoprotein, which is a mixture containing amino acid and vitamin C.
In the present invention, the amino acid is any one of an acidic amino acid, a neutral amino acid, a basic amino acid, and a derivative of an amino acid.
Preferably, in the present invention, the amino acid is a neutral amino acid.
More preferably, in the present invention, the neutral amino acids are cysteine and glycine.
In the invention, the vitamin C is one or more of vitamin C (ascorbic acid) and vitamin C salt (ascorbic acid salt).
Preferably, the vitamin C is a salt of vitamin C.
More preferably, in the present invention, the vitamin C salt is a sodium salt of vitamin C.
In the present invention, the chromoprotein has a structure consisting of a chromophoric group and a protein subunit.
Preferably, the pigment protein is phycocyanin and phycoerythrin.
In the invention, the compound is a uniform mixture obtained by mixing amino acid and vitamin C solid, or mixing amino acid solution and vitamin C solution, or drying the mixed solution.
In the present invention, the drying method may be one of spray drying and freeze drying.
In the invention, the mass ratio of the amino acid to the vitamin C is 1:100-100: 1.
Preferably, in the invention, the mass ratio of the amino acid to the vitamin C is 1:10-10: 1.
More preferably, in the invention, the mass ratio of the amino acid to the vitamin C is 1:5-5: 1.
In the invention, the mass ratio of the amino acid-vitamin C mixture to the pigment protein is 1:50-100: 1.
Preferably, in the invention, the mass ratio of the amino acid-vitamin C mixture to the pigment protein is 1:20-10: 1.
More preferably, in the invention, the mass ratio of the amino acid-vitamin C mixture to the pigment protein is 1:10-5: 1.
In the invention, when the amino acid-vitamin C mixture is compounded with the pigment protein, sugar or sugar alcohol is added as an auxiliary material.
In the invention, when the amino acid-vitamin C mixture is compounded with the pigment protein, the saccharide or the sugar alcohol is one or a mixture of more of monosaccharide, disaccharide or oligosaccharide.
Preferably, in the invention, when the amino acid-vitamin C mixture is compounded with the pigment protein, the saccharide or the sugar alcohol is any one or more of erythritol, trehalose and xylo-oligosaccharide.
The invention has the beneficial effects that:
the compound for improving the light stability of the chromoprotein, provided by the invention, can eliminate the adverse effect of amino acid on the chromoprotein and provide the photoprotection of the chromoprotein which is obviously superior to that provided by single vitamin C, thereby helping the chromoprotein to maintain natural color and activity. In addition, the method does not need to pretreat the pigment protein, does not change the structure of the pigment protein, and has great practical significance for the application of the pigment protein in cosmetics and foods.
Drawings
FIG. 1 is a graph showing the comparison of the remaining ratio of phycocyanin in different samples.
Detailed Description
The following is a further detailed description of specific embodiments of the invention, but the process of the invention is not limited to the examples described below.
The purity of xenon lamp aging instrument and arginine used in the invention is 99%; glycine with purity of 99%; cysteine with purity of 99%; glutamic acid with purity of 99%; vitamin C with purity of 99%; 99% of sodium ascorbate; 99% of calcium ascorbate; phycocyanin is commercially available Spirulina extract with color value of 18; phycoerythrin is prepared from Porphyra yezoensis by literature method (LvCai, Eurycoma, Red Hair weed phycoerythrin extraction and stability research [ J ]. college university (Nature science edition), 2002,7(1): 15-19.); erythritol, trehalose and xylo-oligosaccharide are commercially available food grade.
The first embodiment is as follows: preparation and comparative test of composition for improving light stability of phycocyanin (see figure 1 in the specification)
First, experiment platform
The xenon lamp aging instrument has the wavelength range of 290-800 nm, the experimental conditions of 340nm, the irradiation intensity of 0.35W/m2, the blackboard temperature of 45 ℃ and the light irradiation for 6 hours, and can simulate the shelf life of 6 months. Color and appearance were visually observed, and absorbance was measured at 620 nm.
Second, light protection effect
Phycocyanin 400ppm dissolved in deionized water, placed at 4 ℃ in the dark as a control, and labeled sample 1;
phycocyanin 400ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 2;
phycocyanin 400ppm and vitamin C100 ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 3;
phycocyanin 400ppm and arginine 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 4;
phycocyanin 400ppm and glycine 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 5;
phycocyanin 400ppm and cysteine 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 6;
phycocyanin 400ppm and glutamic acid 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 7;
phycocyanin 400ppm, vitamin C100 ppm and arginine 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 8;
phycocyanin 400ppm, vitamin C100 ppm and glycine 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 9;
phycocyanin 400ppm, vitamin C100 ppm and cysteine 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain a sample 10;
phycocyanin 400ppm, vitamin C100 ppm and glutamic acid 100ppm, dissolved in deionized water, and subjected to xenon lamp aging to obtain sample 11;
phycocyanin 400ppm and sodium ascorbate 100ppm dissolved in deionized water and subjected to xenon lamp aging to obtain sample 12;
phycocyanin 400ppm, sodium ascorbate 100ppm and cysteine 100ppm, dissolved in deionized water, subjected to xenon lamp aging to obtain sample 13;
after the experiment is finished, the appearance is compared, the absorbance is tested at 620nm, and the phycocyanin residual rate is calculated.
Phycocyanin residual rate (sample absorbance/control sample absorbance) 100%
In the present invention, the absorbance at 560nm is measured in relation to the phycoerythrin example.
Phycoerythrin residual rate (sample absorbance/control sample absorbance) 100%
Example two: preparation of compound for improving light stability of phycocyanin
Weighing 5g of glycine, 0.6g of vitamin C1g, 0.6g of phycocyanin and 92g of xylo-oligosaccharide, dissolving the glycine, the vitamin C1g, the phycocyanin and the xylo-oligosaccharide in 1L deionized water, stirring and dissolving the mixture, pre-freezing the mixture at-45 ℃ and carrying out reduced pressure sublimation at 20 ℃ to obtain mixed freeze-dried powder, wherein the mixture can be applied to any formula of food and cosmetics to provide activity or blue color and luster, and is prepared into an aqueous solution containing 400ppm of phycocyanin, and the residual rate of the phycocyanin is 35 percent according to the.
Example three: preparation of compound for improving light stability of phycocyanin
Weighing 1g of glycine, 1g of sodium ascorbate, 2g of phycocyanin and 96g of trehalose, dissolving the glycine, the sodium ascorbate, the phycocyanin and the trehalose in 1L deionized water, stirring and dissolving the mixture, pre-freezing the mixture at-45 ℃, and sublimating the mixture at 20 ℃ under reduced pressure to obtain mixed freeze-dried powder.
Example four: preparation of compound for improving light stability of phycocyanin
Weighing 1g of cysteine, dissolving in 200ml of deionized water, weighing 5g of vitamin C, dissolving in 200ml of deionized water, mixing a glycine aqueous solution and a vitamin C aqueous solution, then mixing with 600ml of an aqueous solution in which 1.2g of phycocyanin and 92g of erythritol are dissolved, stirring and mixing uniformly, pre-freezing at-45 ℃, and sublimating under reduced pressure at 20 ℃ to obtain the mixed freeze-dried powder. The mixture can be used in any formulation of food and cosmetic to provide active or blue color. Preparing water solution containing 400ppm of phycocyanin, and according to the xenon lamp aging method, the residual rate of phycocyanin is 42%.
Example five: preparation of compound for improving light stability of phycocyanin
Weighing 0.07g of glycine, 7g of vitamin C, 350g of phycocyanin and 20g of xylo-oligosaccharide, dissolving the mixture in 200L deionized water, stirring and dissolving the mixture, pre-freezing the mixture at-45 ℃, and carrying out reduced pressure sublimation at 20 ℃ to obtain mixed freeze-dried powder, wherein the mixture can be applied to any formula of food and cosmetics to provide activity or blue color and luster, and the water solution containing 400ppm of phycocyanin is prepared, and the residual rate of the phycocyanin is 24 percent according to the xenon lamp aging method.
Example six: preparation of compound for improving light stability of phycocyanin
Weighing 7g of cysteine, 0.07g of vitamin C, 0.0707g of phycocyanin, 2g of xylo-oligosaccharide, 3g of trehalose and 5g of erythritol, dissolving in 2L deionized water, stirring for dissolution, pre-freezing at-45 ℃, and sublimation under reduced pressure at 20 ℃ to obtain a mixed freeze-dried powder.
Example seven: preparation of compound for improving light stability of phycocyanin
Weighing 1g of glycine, 10g of vitamin C, 110g of phycocyanin, 15g of trehalose and 9g of erythritol, dissolving the materials in 1L deionized water, stirring and dissolving the materials, wherein the inlet temperature is 120 ℃, the outlet temperature is 90 ℃, and spray drying is carried out to obtain mixed powder.
Example eight: preparation of compound for improving light stability of phycocyanin
10g of cysteine, 1g of vitamin C, 220g of phycocyanin, 5g of xylo-oligosaccharide and 4g of trehalose are weighed and dissolved in 1L deionized water, stirred and dissolved, prefreezed at-45 ℃ and decompressed and sublimated at 20 ℃ to obtain mixed freeze-dried powder, the mixture can be applied to any formula of food and cosmetics to provide activity or blue color, 400ppm of phycocyanin-containing aqueous solution is prepared, and the residual rate of the phycocyanin is 35% according to the xenon lamp aging method.
Example nine: preparation of compound for improving light stability of phycocyanin
2g of cysteine, 1g of calcium ascorbate, 5g of phycocyanin, 5g of xylo-oligosaccharide and 4g of trehalose are weighed and dissolved in 1L deionized water, stirred and dissolved, prefreezed at-45 ℃ and decompressed and sublimated at 20 ℃ to obtain mixed freeze-dried powder, the mixture can be applied to any formula of food and cosmetics to provide activity or blue color, an aqueous solution containing 400ppm of phycocyanin is prepared, and the residual rate of the phycocyanin is 35% according to the xenon lamp aging method.
Example ten: phycoerythrin control solution
Weighing phycoerythrin powder, and preparing a 400ppm water solution with deionized water as a control sample of phycoerythrin, wherein the residual rate is 100%.
Example eleven: phycoerythrin aqueous solution
Weighing phycoerythrin powder and deionized water to prepare aqueous solution with 400ppm content, wherein the phycoerythrin residual rate is 1% according to the xenon lamp aging method.
Example twelve: aqueous solution of phycoerythrin and vitamin C
Weighing phycoerythrin powder, vitamin C powder and deionized water to prepare an aqueous solution with the phycoerythrin content of 400ppm and the vitamin C of 100ppm, wherein the phycoerythrin residual rate is 8% according to the xenon lamp aging method.
Example thirteen: aqueous solution of phycoerythrin and glycine
Weighing phycoerythrin powder, glycine powder and deionized water to prepare an aqueous solution with the phycoerythrin content of 400ppm and the glycine content of 100ppm, wherein according to the xenon lamp aging method, the phycoerythrin residual rate is 2%.
Example fourteen: preparation of compound for improving light stability of phycoerythrin
Weighing 1g of glycine, 1g of vitamin C1g, 4g of phycoerythrin and 92g of trehalose, dissolving the glycine, the vitamin C g, the phycoerythrin and the trehalose in 1L deionized water, stirring and dissolving the mixture, pre-freezing the mixture at-45 ℃ and sublimating the mixture at 20 ℃ under reduced pressure to obtain mixed freeze-dried powder, wherein the mixture can be applied to any formula of food and cosmetics to provide activity or red color and luster, and is prepared into 400ppm phycoerythrin-containing aqueous solution, and the phycoerythrin residual rate is 36 percent according to the xenon lamp aging method.
Example fifteen: preparation of compound for improving light stability of phycoerythrin
Weighing 1g of cysteine, dissolving in 200ml of deionized water, weighing 1g of vitamin C, dissolving in 200ml of deionized water, mixing a glycine aqueous solution and a vitamin C aqueous solution, then mixing with 600ml of an aqueous solution in which 2g of phycoerythrin and 92g of erythritol are dissolved, stirring and mixing uniformly, prefreezing at-45 ℃, and sublimating under reduced pressure at 20 ℃ to obtain the mixed freeze-dried powder. The mixture can be used in any formulation of food and cosmetic to provide active or red color. Preparing water solution containing 400ppm of phycoerythrin, and according to the xenon lamp aging method, the residual rate of phycoerythrin is 35%.
Example sixteen: preparation of compound for improving light stability of phycoerythrin
Weighing 2g of cysteine, 1g of calcium ascorbate, 5g of phycoerythrin, 5g of xylo-oligosaccharide and 4g of trehalose, dissolving in 200ml of deionized water, stirring for dissolving, prefreezing at-45 ℃, and sublimating under reduced pressure at 20 ℃ to obtain the mixed freeze-dried powder. The mixture can be used in any formulation of food and cosmetic to provide active or red color. Preparing water solution containing 400ppm of phycoerythrin, and according to the xenon lamp aging method, the residual rate of phycoerythrin is 31%.
Example seventeen: application of compound for improving light stability of phycocyanin in food
Table 1: preparation method of phycocyanin beverage
The preparation method comprises the following steps: accurately weighing each component, dissolving in water, sterilizing, and packaging.
And (3) taking the finished product to be subjected to xenon lamp aging, wherein the phycocyanin residual rate is 32% after the experiment is finished.
Example eighteen: application of compound for improving light stability of phycocyanin in cosmetics
Table 2: preparation of phycocyanin skin care lotion
The preparation method comprises the following steps: dispersing xanthan gum and hyaluronic acid in water for 30min, adding other components, and dissolving to obtain phase A; weighing B, C two phases respectively according to the above proportion, stirring, slowly adding phase B to phase A under stirring, adding phase C after stirring, and stirring to dissolve to obtain phycocyanin skin caring water.
And (3) taking the finished product to be subjected to xenon lamp aging, wherein the phycocyanin residual rate is 34% after the experiment is finished.
Example nineteenth: application of compound for improving light stability of phycoerythrin in food
Table 1: preparation method of phycoerythrin beverage
The preparation method comprises the following steps: accurately weighing each component, dissolving in water, sterilizing, and packaging.
And (3) taking the finished product to be subjected to xenon lamp aging, wherein the phycoerythrin residual rate is 31% after the experiment is finished.
Example twenty: application of compound for improving light stability of phycoerythrin in cosmetics
Table 2: preparation of phycoerythrin skin care lotion
The preparation method comprises the following steps: dispersing xanthan gum and hyaluronic acid in water for 30min, adding other components, and dissolving to obtain phase A; weighing B, C two phases respectively according to the above proportion, stirring, slowly adding phase B to phase A under stirring, adding phase C after stirring, and stirring to dissolve to obtain phycoerythrin skin caring lotion.
And (3) taking the finished product to be subjected to xenon lamp aging, wherein the phycoerythrin residual rate is 32% after the experiment is finished. The food and the cosmetic containing the compound for improving the light stability of the chromoprotein have the color retention of more than 30 percent through a xenon lamp aging experiment simulating the shelf life of 6 months. The amino acid does not improve the light stability of the pigment protein, and even part of the amino acid can promote the degradation of the pigment protein; the vitamin C has a certain effect of improving the light stability of the pigment protein, but is not obvious; when the amino acid and the vitamin C are mixed to form a mixture, the light stability of the pigment protein can be obviously improved, the effect is superior to that of the single amino acid or the vitamin C, the light stability of the pigment protein is effectively improved, and the shelf life of food or cosmetics is prolonged.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the design spirit of the present invention should fall within the protection scope determined by the present invention.
Claims (3)
1. The application of the compound in improving the light stability of the pigment protein is characterized in that the compound is a mixture containing one or more of amino acid, vitamin C and vitamin C salt, the amino acid is glycine, the pigment protein is phycocyanin and phycoerythrin, and the mass ratio of the mixture of one or more of the amino acid, the vitamin C and the vitamin C salt to the pigment protein is 1:10-10: 1; the mass ratio of the amino acid to the vitamin C and one or more of the vitamin C salts is 1:10-10: 1.
2. The use of a complex of claim 1 for improving the photostability of a chromoprotein, wherein said amino acid-vitamin C mixture is complexed with a chromoprotein, and a saccharide or sugar alcohol is added as an adjuvant.
3. Use of a complex according to claim 1 to increase the photostability of a chromoprotein in a food or cosmetic product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710060788.9A CN106943310B (en) | 2017-01-25 | 2017-01-25 | Compound for improving light stability of chromoprotein and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710060788.9A CN106943310B (en) | 2017-01-25 | 2017-01-25 | Compound for improving light stability of chromoprotein and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106943310A CN106943310A (en) | 2017-07-14 |
| CN106943310B true CN106943310B (en) | 2020-07-17 |
Family
ID=59466112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710060788.9A Active CN106943310B (en) | 2017-01-25 | 2017-01-25 | Compound for improving light stability of chromoprotein and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106943310B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3893832A1 (en) | 2018-12-14 | 2021-10-20 | Mary Kay, Inc. | Cosmetic compositions |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2012134605A (en) * | 2012-08-13 | 2014-02-20 | Государственное научное учреждение Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции Российской академии сельскохозяйственных наук | METHOD FOR PRODUCING FOOD ADDITIVES |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5347572A (en) * | 1976-10-08 | 1978-04-28 | Shimizugen Shiyouten Kk | Food coloring method |
| CN1047076C (en) * | 1993-04-28 | 1999-12-08 | 吉富制药株式会社 | Stabilized injection and method of stabilizing injection |
| CN1161812A (en) * | 1997-03-17 | 1997-10-15 | 张福祥 | Vitamin C enriched soda water with effect of ascobic aldehyde |
| US7691388B2 (en) * | 2006-03-24 | 2010-04-06 | Ocean Nutrition Canada Limited | Compositions comprising Porphyra and methods of making and using thereof |
| CN101862332A (en) * | 2009-04-20 | 2010-10-20 | 兰章华 | Composition for preventing human body injuries by excessive consumption of alcohol and preparation method and application thereof |
| CN102675909A (en) * | 2011-01-06 | 2012-09-19 | 上海市食品研究所 | Hemoglobin subunit haematochrome and preparation method thereof |
-
2017
- 2017-01-25 CN CN201710060788.9A patent/CN106943310B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2012134605A (en) * | 2012-08-13 | 2014-02-20 | Государственное научное учреждение Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции Российской академии сельскохозяйственных наук | METHOD FOR PRODUCING FOOD ADDITIVES |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106943310A (en) | 2017-07-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106667794B (en) | Inclusion compound for improving photostability of pigment protein and application thereof | |
| CN104783292A (en) | Lycium ruthenicum Murr effervescent tablet and preparation method thereof | |
| JP6170153B2 (en) | Hair dye products | |
| Yazdi et al. | Biocomponents and antioxidant activity of Ribes khorasanicum | |
| Zorzenon et al. | Spray drying encapsulation of stevia extract with maltodextrin and evaluation of the physicochemical and functional properties of produced powders | |
| KR20160021676A (en) | Cosmetic Compositions with Whitening and Anti-oxidative Effect Comprising of Phragmitis Rhizoma, Salicomia herbacea L. and Prunus mume | |
| JP5632555B1 (en) | Anthocyanin stabilizer and method for stabilizing anthocyanins | |
| CN106943310B (en) | Compound for improving light stability of chromoprotein and application | |
| CN105708731B (en) | Biological microcapsule with whitening effect and preparation method thereof | |
| CN107751948A (en) | Bamboo perfume container salt and preparation method thereof | |
| Kim et al. | Effects of blanching on antioxidant activity and total phenolic content according to type of medicinal plants | |
| JP2904974B2 (en) | Gardenia yellow pigment fading inhibitor | |
| CN114272273A (en) | NMN-containing antioxidant composition and preparation method thereof | |
| JP4190709B2 (en) | Anti-fading agent for dyes | |
| KR20130121385A (en) | A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same | |
| JP5171080B2 (en) | Composition having antioxidative action | |
| CN105315706A (en) | Natural pigment color fixative as well as preparation method and application thereof | |
| CN107114786B (en) | Method for improving stability of blackberry anthocyanin | |
| ES2779984B2 (en) | Procedure to identify properties of a grape extract | |
| Elbandy et al. | Stability of betalain pigments from red beetroot (Beta vulgaris) | |
| Glevitzky et al. | Efficient use of antioxidants to preserve fruit juice | |
| KR102599300B1 (en) | Antioxidant and anti-hair loss functional Ink composition for coloring the scalp containing natural extracts as active ingredients | |
| EP4241779A1 (en) | Aronia extracts and uses thereof | |
| Lukaszewska et al. | Innovative model two-phase makeup remover containing of Jerusalem artichoke tubers extract | |
| CN108740569A (en) | A kind of mixing fish meal and preparation method with feed addictive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210422 Address after: 201901 room a3155, building 1, 947 Jinle Road, Baoshan District, Shanghai Patentee after: Shanghai Xianke Biotechnology Co.,Ltd. Address before: 710000 Shaanxi province Xi'an Beilin District No. 50 grassland Puojia Kyushu garden fifth building 2 unit 10 room 21002 Patentee before: XI'AN KEYISHI BIOTECHNOLOGY Co.,Ltd. |