CN106942240A - A kind of microbicide compositions comprising emodin derivates and BIT - Google Patents
A kind of microbicide compositions comprising emodin derivates and BIT Download PDFInfo
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- CN106942240A CN106942240A CN201710112986.5A CN201710112986A CN106942240A CN 106942240 A CN106942240 A CN 106942240A CN 201710112986 A CN201710112986 A CN 201710112986A CN 106942240 A CN106942240 A CN 106942240A
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- emodin
- aloe
- physcion
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- Health & Medical Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of microbicide compositions comprising emodin derivates and BIT, said composition includes two kinds of effective constituents As and B, active component A is one kind in Physcion or aloe-emodin, active component B is that the weight ratio between the structural compounds with formula (I), two components is 10~1:1~50.Present invention also offers the Preparation method and use of said composition.Result of the test shows that the above-mentioned bactericidal composition synergy that the present invention is provided is obvious, it is often more important that amount of application is reduced, use cost reduction.The bactericidal composition can effectively prevent and treat some specific bacteriums or the fungal disease of crop.By the way that the bactericide of different mechanism of action and the mode of action is compounded, there is effect well in terms of germ resistance and raising prevention effect for expanding fungicidal spectrum and delaying.In formula (I), R is selected from H or C1~C8Alkyl.
Description
Technical field
It is more particularly to a kind of that there is the bactericidal composition for improving performance, tool the invention belongs to agriculture plant protection field
Say it is to be related to a kind of bactericidal composition comprising emodin derivates and BIT body.
Background technology
Physcion (Physcion) and aloe-emodin (Aloeemodin) belong to anthraquinone analog compound.In medicine
These compounds of field are widely used, such as immunoregulation effect, scavenging activated oxygen effect, antibacterial, anti-inflammatory, antiviral work
With diuresis, the protective effect damaged to cerebrum ischemia, cardiovascu- lar effects, antitumor action.In recent years, these anthracenes
Quinones is just progressively applied to agriculture field, for preventing and treating fungi or bacterial disease.Such compound is from medium-height grass
The class highly active vegetable source bactericide extracted in the root of medicine plant rheum officinale, stem, belongs to anthraquinone analog compound, to veterinary antibiotics, paddy
The multiple diseases such as powdery mildew, downy mildew, gray mold, the anthracnose of thing etc. have good prevention effect, while to people and animals' low toxicity,
Environmentally friendly, with other chemical agent no interactions resistances, with preferable uptake and translocation effect, lasting effect is strong.Its sterilization is made
It is the biosynthesis by disturbing disease fungus cell membrane chitin with mechanism, suppresses sprouting, the life of mycelia of pathogen spore
The long, formation of haustorium, so as to block spreading for disease, also can induce crop and produces degeneration-resistant defense reaction, reach the effect of diseases prevention.
BIT class compound is a kind of new, wide-spectrum bactericide, is mainly used in preventing and treating and treating Cereal
The various bacterias such as crop, veterinary antibiotics, fungal disease.Its bactericidal action mechanism, it is main to include destruction germ nucleus knot
Structure, makes it lose cardia and dead and interference germ cell the metabolism of exhaustion, makes its physiologic derangement, ultimately results in dead
Die two aspects.In disease early period of origination using can effectively protect plant not infected by pathogen, disease takes the circumstances into consideration to increase after occurring
Dosage can substantially control spreading for germ, so as to reach protection and the double action rooted out.
Actual agricultural chemicals experience have shown that, repeat and single-minded apply a kind of reactive compound to prevent and treat harmful fungoid very
It will cause the fast selective of fungal bacterial strain in the case of many, it is current usual for the danger of reduction refractory fungal bacterial strain selectivity
Harmful fungoid is prevented and treated using the mixture of different activities compound.By the way that the reactive compound with the different mechanisms of action is entered
Row combination, can delaying drug resistance generation, reduction amount of application, reduction cost accounting.
The content of the invention
The purpose of the present invention is to be directed to bactericide resistance and pedo relict problem in actual applications, filters out difference and kills
The bactericide of bacterium principle is compounded, and obtains a kind of new microbicide compositions, to improve bactericidal agent for preventing and treating effect, delaying drug resistance
Produce, reduce amount of application, reduce cost accounting.
It is a further object to provide the preparation method comprising two kinds of effective constituents As and B bactericidal compositions and
Application in terms of agriculture field preventing and treating corps diseases.
The purpose of the present invention can be reached by following measures：
A kind of microbicide compositions, described composition includes two kinds of active components of A and B, and wherein active component A is rheum officinale
One kind in plain derivative in Physcion and aloe-emodin, wherein active component B are the structuring with formula (I)
Weight ratio between compound, two components is 10~1:1~50.
In formula (I), R is selected from H or C1~C8Alkyl.
C in the present invention1~C8Alkyl refers to the straight or branched alkyl containing 1 to 8 carbon atom, and it includes C1Alkyl
(such as methyl), C2Alkyl (such as ethyl), C3Alkyl (such as n-propyl, isopropyl), C4Alkyl (such as normal-butyl, isobutyl group, the tert-butyl group,
Sec-butyl), C5Alkyl (such as n-pentyl), C6Alkyl, C7Alkyl, C8Alkyl.It includes but is not limited to C1~C6Alkyl, C1~C5
Alkyl, C1~C4Alkyl etc..
In a kind of preferred scheme, R is selected from H or C1~C4Alkyl.
In a kind of preferred scheme, R is selected from H ,-CH3Or-C4H9。
In formula (I), when R is H, A is BIT (abbreviation BIT in specification).
In formula (I), when R is CH3When, A is 2- methyl isophthalic acids, 2- benzisothiazole-3-ketones (abbreviation MBIT in specification).
In formula (I), when R is C4H9When, A is that " butyl " in 2- butyl-BIT, the formula is preferred
Normal-butyl (abbreviation BBIT in specification).
Inventor is found by experiment that composition of the invention is used to prevent and treat crops bacterium or fungal disease, prevents and treats
Synergy is obvious, it is often more important that amount of application is reduced, and reduces use cost.Compound structure type containing component A and component B is not
Together, mechanism of action is different, and both can expand fungicidal spectrum at compounding, and can delay the production of pathogen resistance to a certain extent
No interactions resistance between raw and development speed, and component A and component B.
In a kind of preferred scheme, active component A and active component B weight ratio are 1:1~40, to make action effect more
Substantially both weight ratios can be more preferably 1:1~30.It is still more preferably Physcion and 1,2- benzisothiazoles
The weight ratio of quinoline -3- ketone is 1:1~30.
In a kind of preferred scheme, the weight ratio between A and the components of B two arbitrarily can enter in the range of following proportionings
Row adjustment 10:1、9:1、8:1、7:1、6:1、5:1、4:1、3:1、2:1、1:1、1:2、1:3、1:4、1:5、1:6、1:7、1:8、1:
9、1:10、1:11、1:12、1:13、1:14、1:15、1:16、1:17、1:18、1:19、1:20、1:21、1:22、1:23、1:24、
1:25、1:26、1:27、1:28、1:29、1:30、1:31、1:32、1:33、1:34、1:35、1:36、1:37、1:38、1:39、1:
40、1:41、1:42、1:43、1:44、1:45、1:46、1:47、1:48、1:49、1:50, can also be in two proportionings of any of the above
Selected in the range of constituting, these ratios can be understood as weight ratio, mol ratio can also be included.
The formulation allowed on agricultural chemicals can be made by active component and insecticides adjuvant or auxiliary material in the composition of the present invention.Enter one
Step, said composition constitutes agriculture by the active component of 1~85% parts by weight and the insecticides adjuvant of 99~15% parts by weight or auxiliary material
The formulation allowed on medicine.
The invention provides the bactericidal composition comprising component A and component B in terms of agriculture field preventing and treating corps diseases
Purposes, the particularly purposes in terms of the fungi of some crops or bacteriosis is prevented and treated.
Above-mentioned composition can specifically include insecticides adjuvant or auxiliary material, for example carrier, solvent, dispersant, wetting agent, gluing
One or more in agent, thickener, adhesive, surfactant or fertilizer etc..It can be mixed during administration conventional
Auxiliary agent.
Suitable auxiliary agent or auxiliary material can be solid or liquid, and they are typically the material commonly used during formulation,
For example natural or regeneration mineral matter, solvent, dispersant, wetting agent, adhesive, thickener, adhesive.
The application process of the present composition includes being used for the composition of the present invention into the aerial part of plant growth, especially
It is leaf portion or blade face.It can select to soak seed or be applied to controlling object surface.The frequency and amount of application of administration depend on pathogen
Biology and weather life condition.The growth place of plant, such as rice field can be soaked with the liquid preparation of composition, or
Composition is applied in soil by person in solid form, such as in granular form (soil application), and composition can be by soil through planting
Thing root enters in plant (systemic action).
The composition of the present invention can be prepared into acceptable various formulations on agricultural chemicals, including but not limited to missible oil, suspension
Agent, wettable powder, water dispersible granules, pulvis, granula, aqua, aqueous emulsion, microemulsion, encapsulated form, mother liquor, female powder etc.,
In a kind of preferred scheme, formulation of the invention uses suspending agent, aqua, wettable powder or aqueous emulsion.According to these compositions
Property and the purpose and ambient conditions to be reached using composition, can select composition to spray, atomizing, spray
Powder, broadcast sowing or sprinkle the method poured or the like and apply.
When preparing liquid dosage form, active component B first can be dissolved in alkaline matter, form benzisothiazole metal
Salt, suitable alkaline matter includes：Alkali carbonate, alkali metal hydroxide (such as NaOH, potassium hydroxide), alkali metal
Alkoxy carbonate, alkali metal alcoholates or magnesium methoxide.
The composition of the present invention can be prepared into various formulations with known method, can be by active ingredient with helping
Agent, such as solvent, solid carrier, it is necessary to when can uniformly mix, grind together with surfactant, be prepared into required agent
Type.
Above-mentioned solvent may be selected from aromatic hydrocarbon, preferably containing 8-12 carbon atom, such as xylene mixture or substituted benzene, phthalein
The octanoic acid of esters of gallic acid, such as Dibutyl phthalate or phthalandione two, such as fat hydrocarbon, thiacyclohexane or paraffin, alcohol and ethylene glycol and their ether
And ester, such as ethanol, ethylene glycol, ethylene glycol monomethyl；Ketone, such as cyclohexanone, such as highly polar solvent, N- methyl -2- pyrrolidines
Ketone, dimethyl sulfoxide (DMSO) or dimethylformamide, and vegetable oil or vegetable oil, such as soybean oil.
Above-mentioned solid carrier, is such as used for the typically natural mineral fillers, such as talcum, kaolinite of pulvis and dispersible dose
Soil, montmorillonite or atlapulgite.In order to manage the physical property of composition, polymolecularity silicic acid or polymolecularity can also be added
Adsorpting polymerization thing carrier, such as granulated adsorptive carriers or non-adsorbed carrier, suitable granulated adsorptive carriers are porous types, such as floating
Stone, bentonite or bentonite；Suitable non-adsorbed carrier such as calcite or sand.Further, it is possible to use substantial amounts of inorganic in nature or having
The prefabricated material granulated of machine property is used as carrier, particularly dolomite.
The chemical property of active ingredient in the composition of the present invention, suitable surfactant is sulfomethylated lignin
Acid, naphthalene sulfonic acids, phenolsulfonic acid, alkali salt or amine salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, fat
The condensation product of alcohol sulfate, aliphatic acid and sulphated fatty alcohol glycol ether, also sulfonated naphthalene and naphthalene derivatives and formaldehyde, naphthalene or
Naphthalene sulfonic acids and phenol and the condensation product of formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol,
Alkylaryl polyglycol ether, tributyl benzene polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol,
Ethoxylated castor oil, polyoxyethylene alkyl ether, ethylene oxide condensation product, ethoxylation polyoxypropylene, bay acid polyethylene glycol
Ether acetal, sorbitol ester, lignin sulfite waste liquor and methylcellulose.
Two kinds of active ingredients show as synergistic effect in the composition of the present invention, and the activity of said composition is than using individualized
The expected summation of activity of compound, and the independent activity of single compound are more notable.Synergistic effect shows as allowing amount of application
Reduce, broader antifungal control spectrum, instant effect, more longlasting prevention effect, by only once or a small number of several times using more
Good control plant pest fungi and broaden the possible administration interval time.These characteristics are plant epiphyte control practices
During especially need.
In terms of the microbicide compositions of the present invention can be applied to agriculture field preventing and treating corps diseases, farming referred herein
Thing disease particularly uncinula necator, powdery mildew of cucumber, cucumber downy mildew, balsam pear powdery mildew, graw mold of tomato, wheat white powder
Disease, rice blast.But the present invention composition in addition to it can prevent and treat foregoing disease, moreover it is possible to the specific illness of preventing and treating include but
It is not limited to wheat diseases (such as：Wheat scab, wheat powdery mildew, wheat rust etc.), rice disease (such as：Rice sheath blight disease, water
Rice false smut, paddy bacterial streak etc.), maize diseases (such as：Smut of maize, the leaf blight of corn, corn southern leaf blight), cucumber
Disease is (such as：Powdery mildew of cucumber, cucumber anthracnose, gray mold of cucumber, cucumber bacterial angular leaf spot etc.), tomato disease (such as：Tomato
Powdery mildew, anthracnose of tomato, tomato virus disease, tomato bacterial angular leaf spot etc.), grape disease (such as：Downy mildew of garpe, grape
Gray mold), apple disease (such as：Ring rot of apple, apple anthracnose etc.), citrus disease (such as：Citrus scab simultaneously, citrus anthrax
Disease), tobacco diseases (such as：Wildfire, Alternaria alternate), rape disease (such as：Sclerotinia rot of colza), cotton disease (such as：Cotton
Flower verticillium wilt, cotton wilt) etc..
Other features for showing of bactericidal composition of the present invention are mainly：1st, composition mixture of the invention has obvious
Synergistic effect；2nd, because two single dose chemical constitutions of this composition are widely different, the mechanism of action is entirely different, in the absence of friendship
Mutual resistance, can delay two single doses that produced resistance problem is used alone；3rd, composition of the invention is to crop safety, preventive effect
It is good.Tests prove that, microbicide compositions stable chemical nature of the present invention, synergy significantly, is shown significantly to controlling object
Synergy and complementation.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein is not used to limit only to explain the present invention
The fixed present invention, any modification, equivalent and the improvement made within the spirit and principles of the invention etc., it should be included in
Within protection scope of the present invention.
Percentage is weight percentage in all formulas of following examples.The processing work of the various preparations of the present composition
Skill is prior art, can be varied from according to different situations.
First, formulation prepares embodiment
(1) processing of aqua and embodiment
It is mixed by the ratio of formula with each component such as surfactant and water by active component activity packet A and active component B
Conjunction is uniformly got product.
(1) active component A (Physcion) prepares aqua with active component B (BIT)
Embodiment 1：2.2% Physcion BIT aquas (10:1)
Physcion 2%, BIT 0.2%, NONIN HS 240 6.28%, ethoxylated castor oil
7.53%, water complements to 100%.
Embodiment 2：5.25% Physcion BIT aquas (1:20)
Physcion 0.25%, BIT 5%, tributyl benzene polyglycol ether 8.63%, water complements to 100%.
(2) active component A (Physcion) and active component B (MBIT)) prepare aqua
Embodiment 3：2.2% Physcion MBIT aquas
Physcion 2%, MBIT 0.2%, remaining component is prepared according to the method for embodiment 1.
Embodiment 4：5.25% Physcion MBIT aquas
Physcion 0.25%, MBIT 5%, remaining component is prepared according to the method for embodiment 2.
(3) active component A (Physcion) prepares aqua with active component B (BBIT)
Embodiment 5：2.2% Physcion BBIT aquas
Physcion 2%, BBIT 0.2%, remaining component is prepared according to the method for embodiment 1.
Embodiment 6：5.25% Physcion BBIT aquas
Physcion 0.25%, BBIT 5%, remaining component is prepared according to the method for embodiment 2.
(4) active component A (aloe-emodin) prepares aqua with active component B (BIT)
Embodiment 7：2.2% aloe-emodin BIT aquas
Aloe-emodin 2%, BIT 0.2%, remaining component is prepared according to the method for embodiment 1.
Embodiment 8：5.25% aloe-emodin BIT aquas
Aloe-emodin 0.25%, BIT 5%, remaining component is prepared according to the method for embodiment 2.
(5) active component A (aloe-emodin) prepares aqua with active component B (MBIT)
Embodiment 9：2.2% aloe-emodin MBIT aquas
Aloe-emodin 2%, MBIT 0.2%, remaining component is prepared according to the method for embodiment 1.
Embodiment 10：5.25% aloe-emodin MBIT aquas
Aloe-emodin 0.25%, MBIT 5%, remaining component is prepared according to the method for embodiment 2.
(6) active component A (aloe-emodin) prepares aqua with active component B (BBIT)
Embodiment 11：2.2% aloe-emodin BBIT aquas
Aloe-emodin 2%, BBIT 0.2%, remaining component is prepared according to the method for embodiment 1.
Embodiment 12：5.25% aloe-emodin BBIT aquas
Aloe-emodin 0.25%, BBIT 5%, remaining component is prepared according to the method for embodiment 2.
(2) processing of wettable powder and embodiment
Active components A and B activity composition and various auxiliary agents and filler etc. are sufficiently mixed in proportion, through ultrafine crusher powder
Obtained wettable powder after broken.
(1) active component A (Physcion) prepares wettable powder with active component B (BIT)
Embodiment 13：6% Physcion BIT wettable powders (1:1)
Physcion 3%, BIT 3%, xanthans 1.27%, naphthalene sulfonate salt 5.24%, spaonin powder 4.62%, sulfuric acid
Ammonium 4.79%, kaolin complements to 100%.
Embodiment 14：15.5% Physcion BIT wettable powders (1:30)
Physcion 0.5%, BIT 15%, aluminium-magnesium silicate 4.11%, lauryl sodium sulfate salt 6.52%, alkyl phenol
APEO 5.15%, sodium acid carbonate 3.54%, white carbon supply to 100%.
(2) active component A (Physcion) prepares wettable powder with active component B (MBIT)
Embodiment 15：6% Physcion MBIT wettable powders
Physcion 3%, MBIT 3%, remaining component is prepared according to the method for embodiment 13.
Embodiment 16：15.5% Physcion MBIT wettable powders
Physcion 0.5%, MBIT 15%, remaining component is prepared according to the method for embodiment 14.
(3) active component A (Physcion) prepares wettable powder with active component B (BBIT)
Embodiment 17：6% Physcion BBIT wettable powders
Physcion 3%, BBIT 3%, remaining component is prepared according to the method for embodiment 13.
Embodiment 18：15.5% Physcion BBIT wettable powders
Physcion 0.5%, BBIT 15%, remaining component is prepared according to the method for embodiment 14.
(4) active component A (aloe-emodin) prepares wettable powder with active component B (BIT)
Embodiment 19：6% aloe-emodin BIT wettable powders
Aloe-emodin 3%, BIT 3%, remaining component is prepared according to the method for embodiment 13.
Embodiment 20：15.5% aloe-emodin BIT wettable powders
Aloe-emodin 0.5%, BIT 15%, remaining component is prepared according to the method for embodiment 14.
(5) active component A (aloe-emodin) prepares wettable powder with active component B (MBIT)
Embodiment 21：6% aloe-emodin MBIT wettable powders
Aloe-emodin 3%, MBIT 3%, remaining component is prepared according to the method for embodiment 13.
Embodiment 22：15.5% aloe-emodin MBIT wettable powders
Aloe-emodin 0.5%, MBIT 15%, remaining component is prepared according to the method for embodiment 14.
(6) active component A (aloe-emodin) prepares wettable powder with active component B (BBIT)
Embodiment 23：6% aloe-emodin BBIT wettable powders
Aloe-emodin 3%, BBIT 3%, remaining component is prepared according to the method for embodiment 13.
Embodiment 24：15.5% aloe-emodin BBIT wettable powders
Aloe-emodin 0.5%, BBIT 15%, remaining component is prepared according to the method for embodiment 14.
(3) processing of aqueous emulsion and embodiment
Active components A and B activity composition and solvent, emulsifying agent, dispersant, antifreeze etc. are sufficiently mixed in proportion, plus
It is well mixed after water and can be prepared by aqueous emulsion.
(1) active component A (Physcion) prepares aqueous emulsion with active component B (BIT)
Embodiment 25：2.75% Physcion BIT aqueous emulsions (1:10)
Physcion 0.25%, BIT 2.5%, dense newborn 600 6.32%, toluene 10%, calcium dodecyl benzene sulfonate
5.25%, ammonium sulfate 5%, water complements to 100%.
Embodiment 26：10.25% Physcion BIT aqueous emulsions (1:40)
Physcion 0.25%, BIT 10%, APES formaldehyde condensation product 5.43%, dimethylbenzene
15.0%, spaonin powder 8.62%, glycerine 10%, water complements to 100%.
Embodiment 27：5.1% Physcion BIT aqueous emulsions (1:50)
Physcion 0.1%, BIT 5%, Tween-80 5.87%, dimethylbenzene 8.0%, lauryl sodium sulfate
4.65%, propane diols 6%, water complements to 100%.
(2) active component A (Physcion) prepares aqueous emulsion with active component B (MBIT)
Embodiment 28：2.75% Physcion MBIT aqueous emulsions
Physcion 0.25%, MBIT 2.5%, remaining component is prepared according to the method for embodiment 25.
Embodiment 29：10.25% Physcion MBIT aqueous emulsions
Physcion 0.25%, MBIT 10%, remaining component is prepared according to the method for embodiment 26.
Embodiment 30：5.1% Physcion MBIT aqueous emulsions
Physcion 0.1%, MBIT 5%, remaining component is prepared according to the method for embodiment 27.
(3) active component A (Physcion) prepares aqueous emulsion with active component B (BBIT)
Embodiment 31：2.75% Physcion BBIT aqueous emulsions
Physcion 0.25%, BBIT 2.5%, remaining component is prepared according to the method for embodiment 25.
Embodiment 32：10.25% Physcion BBIT aqueous emulsions
Physcion 0.25%, BBIT 10%, remaining component is prepared according to the method for embodiment 26.
Embodiment 33：5.1% Physcion BBIT aqueous emulsions
Physcion 0.1%, BBIT 5%, remaining component is prepared according to the method for embodiment 27.
(4) active component A (aloe-emodin) prepares aqueous emulsion with active component B (BIT)
Embodiment 34：2.75% aloe-emodin BIT aqueous emulsions
Aloe-emodin 0.25%, BIT 2.5%, remaining component is prepared according to the method for embodiment 25.
Embodiment 35：10.25% aloe-emodin BIT aqueous emulsions
Aloe-emodin 0.25%, BIT 10%, remaining component is prepared according to the method for embodiment 26.
Embodiment 36：5.1% aloe-emodin BIT aqueous emulsions
Aloe-emodin 0.1%, BIT 5%, remaining component is prepared according to the method for embodiment 27.
(5) active component A (aloe-emodin) prepares aqueous emulsion with active component B (MBIT)
Embodiment 37：2.75% aloe-emodin MBIT aqueous emulsions
Aloe-emodin 0.25%, MBIT 2.5%, remaining component is prepared according to the method for embodiment 25.
Embodiment 38：10.25% aloe-emodin MBIT aqueous emulsions
Aloe-emodin 0.25%, MBIT 10%, remaining component is prepared according to the method for embodiment 26.
Embodiment 39：5.1% aloe-emodin MBIT aqueous emulsions
Aloe-emodin 0.1%, MBIT 5%, remaining component is prepared according to the method for embodiment 27.
(6) active component A (aloe-emodin) prepares aqueous emulsion with active component B (BBIT)
Embodiment 40：2.75% aloe-emodin BBIT aqueous emulsions
Aloe-emodin 0.25%, BBIT 2.5%, remaining component is prepared according to the method for embodiment 25.
Embodiment 41：10.25% aloe-emodin BBIT aqueous emulsions
Aloe-emodin 0.25%, BBIT 10%, remaining component is prepared according to the method for embodiment 26.
Embodiment 42：5.1% aloe-emodin BBIT aqueous emulsions
Aloe-emodin 0.1%, BBIT 5%, remaining component is prepared according to the method for embodiment 27.
2nd, drug effect checking test
(1) Assays Example
The incidence of whole strain blade is investigated according to experiment grade scale, disease index and prevention effect is calculated.
Prevention effect is converted into niqueMin (y), liquor strength (μ g/ml) is converted into logarithm value (x), with least square method
Concentration EC50 in calculating virulence equation and suppressing, the toxicity index and co-toxicity coefficient (CTC) of medicament are calculated according to the abundant methods of Sun Yun.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
A percentage composition+B medicament toxicity index * mixtures in theoretical toxicity index (TTI)=A medicament toxicity index * mixtures
Middle B percentage composition
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] * 100
CTC≤80, composition shows as antagonism, 80 ＜ CTC ＜ 120, and composition shows as summation action, and CTC >=
120, composition shows as synergistic effect.
1st, Physcion and BIT compoundings are tested capsicum leaf spot toxicity test
The Physcion of table 1. is compounded to capsicum leaf spot toxicity test interpretation of result with BIT
As a result (table 1) shows, in Physcion：BIT=10~1:In 1~50 interval range, Physcion and BIT
Compound and the prevention effect of capsicum leaf spot is significantly improved, illustrate that the two compounding has significant synergy to make the preventing and treating of capsicum leaf spot
With.
2nd, Physcion and MBIT compoundings are tested leaf muld of tomato toxicity test
The Physcion of table 2. is compounded to leaf muld of tomato toxicity test interpretation of result with MBIT
| Medicament title | EC50(ug/mL) | ATI | TTI | CTC |
| Physcion | 2.75 | 100.00 | \ | \ |
| MBIT | 4.89 | 56.24 | \ | \ |
| Physcion：MBIT=20:1 | 2.09 | 131.85 | 97.92 | 134.66 |
| Physcion：MBIT=10:1 | 1.42 | 193.54 | 96.02 | 201.56 |
| Physcion：MBIT=1:1 | 1.56 | 175.74 | 78.12 | 224.96 |
| Physcion：MBIT=1:10 | 1.86 | 147.55 | 60.22 | 245.02 |
| Physcion：MBIT=1:20 | 1.78 | 154.65 | 58.32 | 265.16 |
| Physcion：MBIT=1:30 | 1.98 | 138.76 | 57.65 | 240.68 |
| Physcion：MBIT=1:40 | 2.18 | 125.93 | 57.31 | 219.75 |
| Physcion：MBIT=1:50 | 2.37 | 116.13 | 57.10 | 203.39 |
| Physcion：MBIT=1:60 | 3.72 | 73.89 | 56.96 | 129.72 |
As a result (table 2) shows, Physcion compounds with MBIT and the prevention effect of leaf muld of tomato is significantly improved, explanation
The two compounding has significant synergistic effect to leaf muld of tomato preventing and treating.
3rd, Physcion and BBIT compoundings are tested cucurbita pepo aspergillosis toxicity test
The Physcion of table 3. is compounded to cucurbita pepo aspergillosis toxicity test interpretation of result with BBIT
As a result (table 3) shows, Physcion compounds with BBIT and the prevention effect of cucurbita pepo aspergillosis is significantly improved, and says
The two bright compounding has significant synergistic effect to the preventing and treating of cucurbita pepo aspergillosis.
4th, aloe-emodin and BIT compoundings are tested bacterial fruit blotch of watermelon toxicity test
The aloe-emodin of table 4. is compounded to bacterial fruit blotch of watermelon toxicity test interpretation of result with BIT
| Medicament title | EC50(ug/mL) | ATI | TTI | CTC |
| Aloe-emodin | 2.84 | 100.00 | \ | \ |
| BIT | 3.71 | 76.55 | \ | \ |
| Aloe-emodin：BIT=20:1 | 2.29 | 124.27 | 98.88 | 125.67 |
| Aloe-emodin：BIT=10:1 | 2.12 | 134.23 | 97.87 | 137.15 |
| Aloe-emodin：BIT=1:1 | 2.12 | 134.23 | 88.28 | 152.06 |
| Aloe-emodin：BIT=1:10 | 2.19 | 129.81 | 78.68 | 164.98 |
| Aloe-emodin：BIT=1:20 | 2.05 | 138.67 | 77.67 | 178.54 |
| Aloe-emodin：BIT=1:30 | 2.32 | 122.42 | 77.31 | 158.36 |
| Aloe-emodin：BIT=1:40 | 2.60 | 109.18 | 77.12 | 141.57 |
| Aloe-emodin：BIT=1:50 | 2.84 | 99.84 | 77.01 | 129.65 |
| Aloe-emodin：BIT=1:60 | 3.20 | 88.76 | 76.93 | 115.37 |
As a result (table 4) shows, aloe-emodin compounds with BIT and the prevention effect of bacterial fruit blotch of watermelon is significantly carried
Height, illustrates that the two compounding has significant synergistic effect to bacterial fruit blotch of watermelon preventing and treating.
5th, aloe-emodin and MBIT compoundings are tested muskmelon damping-off toxicity test
The aloe-emodin of table 5. is compounded to muskmelon damping-off toxicity test interpretation of result with MBIT
As a result (table 5) shows, in aloe-emodin：MBIT=10~1:In 1~50 interval range, aloe-emodin with
The co-toxicity coefficient of MBIT compounding muskmelon damping-offs illustrates that the two compounding has significantly to muskmelon damping-off control more than 153.12
Synergistic effect.
6th, aloe-emodin and BBIT compoundings are tested sponge gourd blight dis-ease toxicity test
The aloe-emodin of table 6. is compounded to sponge gourd blight dis-ease toxicity test interpretation of result with BBIT
| Medicament title | EC50(ug/mL) | ATI | TTI | CTC |
| Aloe-emodin | 5.84 | 100.00 | \ | \ |
| BBIT | 8.25 | 70.79 | \ | \ |
| Aloe-emodin：BBIT=20:1 | 4.15 | 140.58 | 98.61 | 142.56 |
| Aloe-emodin：BBIT=10:1 | 2.98 | 196.30 | 97.34 | 201.65 |
| Aloe-emodin：BBIT=1:1 | 3.05 | 191.55 | 85.40 | 224.31 |
| Aloe-emodin：BBIT=1:10 | 3.18 | 183.88 | 73.45 | 250.36 |
| Aloe-emodin：BBIT=1:20 | 2.94 | 198.79 | 72.18 | 275.41 |
| Aloe-emodin：BBIT=1:30 | 3.21 | 181.78 | 71.73 | 253.41 |
| Aloe-emodin：BBIT=1:40 | 3.58 | 162.98 | 71.50 | 227.94 |
| Aloe-emodin：BBIT=1:50 | 4.12 | 141.74 | 71.36 | 198.62 |
| Aloe-emodin：BBIT=1:60 | 5.92 | 98.64 | 71.27 | 138.41 |
As a result (table 6) shows, in aloe-emodin：BBIT=10~1:In 1~50 interval range, aloe-emodin with
BBIT is compounded to the co-toxicity coefficient of sponge gourd blight dis-ease more than 198.62, illustrates that the two compounding has aobvious to the preventing and treating of sponge gourd blight dis-ease
The synergistic effect of work.
(2) field efficacy checking test
Test method：At the initial stage of a disease, first time spraying is carried out immediately, and second of dispenser is carried out after 7 days, 4 are each handled
Individual cell, each square meter of cell 20.In 11 days investigation statisticses incidences before medicine and after second medicine, each 5 points of cell is random
Sampling, 5 plants of crops of every investigation, investigates percentage and classification that the lesion area in whole strain per blade accounts for blade area, calculates
Disease index and prevention effect.
It is expected that preventive effect (%)=X+Y-XY/100 (wherein, X, Y are single dose preventive effect)
Grade scale：
0 grade：Disease-free spot；
1 grade：Leaf spot lesion is less than 5, and length is less than 1cm；
3 grades：Leaf spot lesion 6-10, part scab length is more than 1cm；
5 grades：Leaf spot lesion 11-25, part scab is linked to be piece, and lesion area accounts for the 10-25% of leaf area；
7 grades：Leaf spot lesion more than 26, scab is linked to be piece, and lesion area accounts for the 26-50% of leaf area；
9 grades：Scab is linked to be piece, and more than 50% or the full leaf that lesion area accounts for leaf area are withered.
1st, Physcion and BIT compounding field efficacy experiments
The Physcion of table 7. is with BIT mixtures to downy mildew of garpe prevention effect
Measurement result (table 7) shows that Physcion and BIT compounding are significantly improved to downy mildew of garpe preventive effect, explanation
The two compounding has significant synergistic effect to downy mildew of garpe.
2nd, Physcion and MBIT compounding field efficacy experiments
The Physcion of table 8 is with MBIT mixtures to Prospect on Kiwifruit Bacterial Canker prevention effect
Measurement result (table 8) shows that Physcion is significantly improved with MBIT mixtures to the preventive effect of Prospect on Kiwifruit Bacterial Canker, is said
The two bright compounding has significant synergistic effect to Prospect on Kiwifruit Bacterial Canker.
3rd, Physcion and BBIT compounding field efficacy experiments
The Physcion of table 9 is with BBIT mixtures to late blight of potato prevention effect
Measurement result (table 9) shows that Physcion is significantly improved with BBIT mixtures to the preventive effect of the late blight of potato, is said
The two bright compounding has significant synergistic effect to the late blight of potato.
4th, aloe-emodin and BIT compounding field efficacy experiments
The aloe-emodin of table 10 is with BIT mixtures to wheat scab prevention effect
Measurement result (table 10) shows that aloe-emodin is significantly improved with BIT mixtures to the preventive effect of wheat scab, explanation
The two compounding has significant synergistic effect to wheat scab.
5th, aloe-emodin and MBIT compounding field efficacy experiments
The aloe-emodin of table 11 is with MBIT mixtures to tobacco black shank prevention effect
Measurement result (table 11) shows that aloe-emodin is significantly improved with MBIT mixtures to the preventive effect of tobacco black shank, is said
The two bright compounding has significant synergistic effect to tobacco black shank.
6th, aloe-emodin and BBIT compounding field efficacy experiments
The aloe-emodin of table 12 is with BBIT mixtures to citrus anthracnose prevention effect
Measurement result (table 12) shows that aloe-emodin is significantly improved with BBIT mixtures to the preventive effect of citrus anthracnose, is said
The two bright compounding has significant synergistic effect to citrus anthracnose.
Claims (10)
1. a kind of microbicide compositions, it is characterised in that described composition includes two kinds of active components of A and B, active component A is
One kind in emodin derivates in Physcion and aloe-emodin, active component B is the structuring with formula (I)
Compound, wherein the weight ratio between two components is 10~1:1~50
In formula (I), R is selected from H or C1~C8Alkyl.
2. microbicide compositions according to claim 1, it is characterised in that in formula (I), R is selected from H or C1~C4Alkyl.
3. microbicide compositions according to claim 1, it is characterised in that in formula (I), R is selected from H ,-CH3Or-C4H9, it is right
The active component A answered is respectively 1,2- benzisothiazole-3-ketones, 2- methyl isophthalic acids, 2- benzisothiazole-3-ketones or 2- fourths
Base -1,2- benzisothiazole-3-ketones.
4. microbicide compositions according to claim 1, it is characterised in that active component A and active component B weight ratio
For 1:1~40.
5. microbicide compositions according to claim 4, it is characterised in that active component A and active component B weight ratio
For 1:1~30.
6. microbicide compositions according to claim 1, it is characterised in that Physcion and 1,2- benzisothiazole-
The weight ratio of 3- ketone is 1:1~30.
7. according to any described microbicide compositions in claim 1~6, it is characterised in that said composition is by 1~85% weight
Measure the active component of part and the formulation allowed on the insecticides adjuvant of 99~15% parts by weight or auxiliary material composition agricultural chemicals.
8. microbicide compositions according to claim 7, it is characterised in that described formulation be aqua, wettable powder,
Or aqueous emulsion.
9. microbicide compositions according to claim 7, it is characterised in that the insecticides adjuvant or auxiliary material be selected from carrier, molten
One or more in agent, dispersant, wetting agent, adhesive, thickener, adhesive, surfactant and fertilizer.
10. use of the microbicide compositions in terms of agriculture field prevents and treats corps diseases described in claim 1~9 any one
On the way.
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| PCT/CN2017/116695 WO2018157649A1 (en) | 2017-02-28 | 2017-12-16 | Microbicide composition containing emodin derivative and benzisothiazolinone |
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| WO2018157649A1 (en) * | 2017-02-28 | 2018-09-07 | 江苏辉丰生物农业股份有限公司 | Microbicide composition containing emodin derivative and benzisothiazolinone |
| CN110537548A (en) * | 2018-12-13 | 2019-12-06 | 北京宇悦生物科技有限公司 | Pesticide composition and application thereof |
| CN110537542A (en) * | 2018-12-13 | 2019-12-06 | 北京宇悦生物科技有限公司 | Pesticide composition and application thereof |
| CN112480031A (en) * | 2020-11-26 | 2021-03-12 | 江苏海洋大学 | Emodin amide derivatives containing 1,3, 4-thiadiazole, and synthesis method and application thereof |
| CN113173843A (en) * | 2021-05-19 | 2021-07-27 | 云南民族大学 | Tobacco black shank resistant active compound and preparation method and application thereof |
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Also Published As
| Publication number | Publication date |
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| CN106942240B (en) | 2018-11-23 |
| WO2018157649A1 (en) | 2018-09-07 |
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