CN106942099A - A kind of method of siganus guttatus fry hatching - Google Patents
A kind of method of siganus guttatus fry hatching Download PDFInfo
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- CN106942099A CN106942099A CN201710096448.1A CN201710096448A CN106942099A CN 106942099 A CN106942099 A CN 106942099A CN 201710096448 A CN201710096448 A CN 201710096448A CN 106942099 A CN106942099 A CN 106942099A
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- fry
- embryonated egg
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- siganus guttatus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to fish culture field, specifically a kind of method of siganus guttatus fry hatching comprises the following steps：(1) collection of embryonated egg；(2) preparation of mucus is taken off, embryonated egg is de- glutinous；(3) preparation of hatching barrel and incubating oosperm；(4) temporarily foster equipment prepares and newly hatched larvae is temporarily supported.The advantage of the invention is that：Embryonated egg infringement is few, and incubation rate is high, and newly hatched larvae vigor is good.Technology maturation, safely, be easily mastered, can by raiser's rapid digestion and grasp, and suitable for carry out seed large-scale artificial breed.
Description
Technical field
It is a kind of method of siganus guttatus fry hatching specifically the present invention relates to fish culture field.
Background technology
Siganus guttatus (Siganus guttatus are commonly called as golden tiger spot) is under the jurisdiction of Perciformes, siganidae, siganus,
Its delicious meat, protein content is high, rich in n-3 highly unsaturated fatty acids, is of high nutritive value, deep to be liked by consumers in general.
The features such as sub- fish of filling enamel has omnivorousness, eurysalinity, eurythermic, is also that seawater is supported under field conditions (factors) using macro as food
Rare ominivore-fish in kind is grown, meets the demand of China's current health aquaculture model development.Basket fish is excellent as one kind
Good new cultivation object, exploitation prospect is wide.
The sub- fish egg-laying season fill enamel for the annual 4-7 months, the lunar calendar No. seven to No. nine of every month is laid eggs, and siganus guttatus fish-egg is
Heavy property adhesive egg, the hatching method of regular seawater fry easily makes that fish-egg is blocking to be piled up in together, causes anoxic, son in the middle of pieces of an egg
Fish hardly possible membrane, situations such as newly hatched larvae abnormal rate is high, incubation rate is greatly reduced, cause the loss in production.Siganus guttatus ovum
Grain is small, and only 0.57 ± 0.08mm, newly hatched larvae is smaller weaker, is easily disturbed by external environment, it is therefore desirable to which one more
Stable growth course, makes it be developed to the exogenous nutrition phase.
Therefore, how research provides a kind of guarantee hatchability of fertile eggs in the artificial batch Seedling production of siganus guttatus, abnormal
Form quotient is low, and the good method of newly hatched larvae vigor is necessary.
The content of the invention
It is an object of the invention to provide a kind of method of siganus guttatus fry hatching, including following steps：(1) by
The collection of smart ovum；(2) preparation of mucus is taken off, embryonated egg is de- glutinous；(3) preparation of hatching barrel and incubating oosperm；(4) equipment is temporarily supported
Prepare and newly hatched larvae is temporarily supported.This method has embryonated egg infringement few, and incubation rate height, newly hatched larvae vigor is good, technology maturation, peace
Entirely, it is easily mastered, can be bred by raiser's rapid digestion and grasp, and suitable for carrying out seed large-scale artificial.
The first aspect of the present invention comprises the following steps there is provided a kind of method of siganus guttatus fry hatching：
(A) after parent population natural spawning fertilization, reduction parent fish rearing pool water level to 30cm, adding will after eugenol anesthesia parent population
Parent population is transferred to standby parent fish rearing pond, reuses 100 mesh sieve tulles bag and collects embryonated egg；
(B) embryonated egg after collecting is poured into talcum powder and taken off in mucus, does not stop to stir 5-8min, carries out de- glutinous processing；
(C) by the embryonated egg after de- glutinous processing, after cleaning up, it is transferred to the hatching barrel after disinfecting Nei and is incubated
Change, hatching density is 500g/m3, dead ovum and chorion are removed after fry membrane；
(D) newly hatched larvae is transferred in temporarily foster equipment, temporarily after foster 48h, you can be put into pond, carry out seed rearing.
Parent fish rearing pond uses indoor semi-buried cement pit in described step (A), is injected after culture pond sterilization through husky mistake
Seawater after filter, height of water level is 1m, and salinity 27~32 ‰, the standard of every square metre of 1 gas head installs aerating device, culture pond
Keep available light.
Eugenol produces for Dentistry Material Plant, Shanghai Medical Machinery Co., Ltd. in described step (A), using dense
Spend for 10ppm.
Mesh bag is tied to culture pond discharge outlet when embryonated egg is collected in described step (A), then by flowing water by bottom of pond
Embryonated egg rinse in mesh bag, bolting silk net bag length be 2m, a diameter of 40cm.
The de- mucus of talcum powder is talcum powder and seawater with mass ratio 1 in described step (B):After 40 ratio mixing fully
It is made.
The usage amount that talcum powder takes off mucus in described step (B) is to use 10kg per 1000g embryonated eggs.
Hatching barrel is the high 1.2m of bucket in described step (C), and volume is 0.6m3, effective water body 0.5m3, bucket is interior to install gas head
2, dissolved oxygen keeps ＞ 4mg/L.
Hatching barrel is carried out disinfection processing using 40mg/L liquor potassic permanganates immersion 30min in described step (C).
In described step (C) after fry membrane, stop the supple of gas or steam, stand 5min, energetic fry is floated, and dead ovum and work
Sink to behind hatching barrel bottom and remove under the fry of power difference.
The canvas net cage that temporarily foster equipment is 2.5m × 2.5m × 1m in described step (D), net cage surrounding is buoyant foam
Plate, the canvas provided with sunshade rain-proof installs gas head according to the standard of every square metre of 1 gas head, and dissolved oxygen keeps ＞ 4mg/L, protects
Hold available light；It is fixed in fry seed rearing pond.
The temporarily foster density of newly hatched larvae is 50 × 10 in described step (D)4Tail/m3。
The invention has the advantages that：
The present invention regathers embryonated egg after being shifted by parent population, and the waste and loss of embryonated egg can be reduced to greatest extent；
De- glutinous processing after fertilization ovum collecting, can improve the incubation rate of embryonated egg；Embryonated egg is hatched using special hatching barrel, is incubated
It is transferred to after change in special temporarily foster equipment, improves the survival rate of newly hatched larvae.This technology is ripe, safely, be easily mastered, energy
It is enough to be bred by raiser's rapid digestion and grasp, and suitable for carrying out seed large-scale artificial, it is expected to promote China's point basket
The health of fish artificial seed's breeding process, fast development.
Embodiment
The embodiment provided with reference to embodiment the present invention elaborates.
Embodiment 1
The method of siganus guttatus fry hatching, comprises the following steps：
(1) parent population transfer and the collection of embryonated egg：
On April 14th, 2016,28 DEG C of water temperature, the tail of siganus guttatus parent population 200 is cultivated in indoor cement pit, and morning on the 14th is certainly
So spawning, starts to reduce parent fish rearing pool water level 7 points of morning, because the embryonated egg of siganus guttatus belongs to heavy property adhesive egg, fertilization
Ovum is sunken to water, can be first without using collection mesh bag., can full pool spilling head cloves when water level, which is reduced to parent population dorsal fin, to surface
Phenol, and tie up embryonated egg collection mesh bag.When the fine motion of the siganus guttatus parent population gill cover, turn on one's side, react insensitive after, dragged for using soft net
Ready standby culture pond quickly is transferred to, the embryonated egg positioned at bottom of pond is then collected.
(2) embryonated egg is de- glutinous：
Because parent fish rearing pond parent population is more, after embryonated egg collection mesh bag flowing water is not smooth, it should with changing collection mesh bag.This
2650g embryonated eggs are collected in secondary spawning altogether, and the embryonated egg gathered is poured into ready talcum powder takes off in mucus, does not stop to stir
5min or so.After embryonated egg is no longer sticky in together, is dragged for and be collected in clean seawater with 100 mesh nets, by unnecessary talcum
Powder is cleaned up.
(3) hatching of embryonated egg：
De- glutinous clean embryonated egg is transferred into hatching barrel to be hatched, a hatching barrel hatches 300g embryonated eggs, inflation
Amount is rolled with embryonated egg in water body, and bucket bottom does not have the heavy collection of embryonated egg to be defined.Or so morning on the 15th observes that fry starts membrane,
When turning aeration quantity down after fry hatching membrane, to reduce the injury to newly hatched larvae.
(4) newly hatched larvae is temporarily supported：
Morning on the 15th, before the sun does not come out, newly hatched larvae is transferred to temporarily foster equipment, temporarily after foster 48h, you can be put into
Fry rearing pond, carries out seed rearing.
Hatch results：Parent population survival rate 100%, collects embryonated egg 2650g, is put into 9 hatching barrels and is hatched altogether, is fertilized
Egg hatching rate is 91.50%, and the survival rate of newly hatched larvae is 85.59%.
Reference examples 1
In May, 2015, siganus guttatus parent population natural spawning collects embryonated egg and hatching using conventional method.
(1) damage of parent population is increased
Due to siganus guttatus, stimulate the reaction is fierce to external world, and stress reaction is big, dorsal fin band poison.It is conventional to use in parent fish rearing
The method of suspension spawning net cage, because parent population is focused into net cage, causes parent population mutually to collide in pond, produces stress reaction, the back of the body
Fin opens, and causes parent population body surface badly wounded.No. seven to No. nine spawnings of the lunar calendar of siganus guttatus every month, it is therefore desirable to change by
Smart ovum collecting net cage, in Renewal process, the water surface of running helter-skelter or fly out in net cage after parent population is frightened also brings more machineries
Damage, parent population survival rate is only 81.20%.
(2) incubation rate of embryonated egg is reduced
Siganus guttatus fish-egg is heavy property adhesive egg, and the hatching method of regular seawater fry easily makes that fish-egg is blocking to be piled up in one
Rise, cause the difficult membrane of anoxic in the middle of pieces of an egg, prelarva, situations such as newly hatched larvae abnormal rate is high, greatly reduce incubation rate, cause life
Loss in production, incubation rate is only 65.8%.
(3) survival rate of newly hatched larvae is reduced
Siganus guttatus ovum grain is small, and only 0.57 ± 0.08mm, newly hatched larvae is smaller weaker, is easily done by external environment
Disturb, such as the relatively stable external environment of neither one, it is developed to the exogenous nutrition phase, it will to cause the survival rate of newly hatched larvae
Reduction.
The preferred embodiment to the invention is illustrated above, but the invention be not limited to it is described
Embodiment, those of ordinary skill in the art can also make a variety of equivalent on the premise of without prejudice to the invention spirit
Modification or replacement, these equivalent modifications or replacement are all contained in the application claim limited range.
Claims (7)
1. a kind of method of siganus guttatus fry hatching, it is characterised in that comprise the following steps：
(A) after parent population natural spawning fertilization, reduction parent fish rearing pool water level to 30cm is added after 10ppm eugenols anesthesia parent population
Parent population is transferred to standby parent fish rearing pond, 100 mesh sieve tulles bag is reused and collects embryonated egg；
(B) embryonated egg after collecting is poured into talcum powder and taken off in mucus, does not stop to stir 5-8min, carries out de- glutinous processing；The talcum
The de- mucus of powder is talcum powder and seawater with mass ratio 1:It is made after 40 ratio mixing fully；Talcum powder take off mucus usage amount be
10kg is used per 1000g embryonated eggs；
(C) by the embryonated egg after de- glutinous processing, after cleaning up, it is transferred to the hatching barrel after disinfecting Nei and is hatched, incubated
Change density is 500g/m3, dead ovum and chorion are removed after fry membrane；
(D) newly hatched larvae is transferred in temporarily foster equipment, temporarily foster density is 50 × 104Tail/m3, temporarily after foster 48h, you can be put into pond
The pool, carries out seed rearing.
2. the method for siganus guttatus fry hatching according to claim 1, it is characterised in that：It is close in described step (A)
Fish culture pond uses the seawater that indoor semi-buried cement pit, culture pond are injected after sterilizing after husky filtering, and height of water level is 1m, salt
Degree 27~32 ‰, the standard of every square metre of 1 gas head installs aerating device, and culture pond keeps available light.
3. the method for siganus guttatus fry hatching according to claim 1, it is characterised in that：Received in described step (A)
Mesh bag is tied to culture pond discharge outlet during collection embryonated egg, then rinsed the embryonated egg of bottom of pond in mesh bag by flowing water, bolting silk
Mesh bag length is 2m, a diameter of 40cm.
4. the method for siganus guttatus fry hatching according to claim 1, it is characterised in that：Incubated in described step (C)
Change bucket is the high 1.2m of bucket, and volume is 0.6m3, effective water body 0.5m3, bucket is interior to install first 2 of gas, and dissolved oxygen keeps ＞ 4mg/L.
5. the method for siganus guttatus fry hatching according to claim 1, it is characterised in that：Incubated in described step (C)
Change bucket to carry out disinfection processing using 40mg/L liquor potassic permanganates immersion 30min.
6. the method for siganus guttatus fry hatching according to claim 1, it is characterised in that：Fish in described step (C)
After seedling membrane, stop the supple of gas or steam, stand 5min, energetic fry is floated, and hatching is sunk under the young fry of dead ovum and vigor difference
Removed after bottom of the barrel.
7. the method for siganus guttatus fry hatching according to claim 1, it is characterised in that：In described step (D) temporarily
The canvas net cage that equipment is 2.5m × 2.5m × 1m is supported, net cage surrounding is buoyant foam plate, and the canvas provided with sunshade rain-proof is pressed
Standard according to every square metre of 1 gas head installs gas head, and dissolved oxygen keeps ＞ 4mg/L, keeps available light；It is fixed on the training of fry seed
Educate in pond.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710096448.1A CN106942099A (en) | 2017-02-22 | 2017-02-22 | A kind of method of siganus guttatus fry hatching |
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| CN201710096448.1A CN106942099A (en) | 2017-02-22 | 2017-02-22 | A kind of method of siganus guttatus fry hatching |
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| CN201710096448.1A Pending CN106942099A (en) | 2017-02-22 | 2017-02-22 | A kind of method of siganus guttatus fry hatching |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646763A (en) * | 2017-11-10 | 2018-02-02 | 中国水产科学研究院南海水产研究所 | A kind of macula lutea basket fish artificial fecundation method |
| CN111149735A (en) * | 2020-01-21 | 2020-05-15 | 中国水产科学研究院南海水产研究所深圳试验基地 | Large-scale cultivation method of siganus oramin fry |
| CN113647347A (en) * | 2021-09-17 | 2021-11-16 | 广东海洋大学 | Early cultivation method of marine fish seedlings |
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| CN104335938A (en) * | 2014-09-09 | 2015-02-11 | 中国水产科学研究院东海水产研究所 | Siganus guttatus fry breeding method |
| CN205052505U (en) * | 2015-09-17 | 2016-03-02 | 通威股份有限公司 | Novel fry hatching, cultivation pond |
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| CN104335938A (en) * | 2014-09-09 | 2015-02-11 | 中国水产科学研究院东海水产研究所 | Siganus guttatus fry breeding method |
| CN205052505U (en) * | 2015-09-17 | 2016-03-02 | 通威股份有限公司 | Novel fry hatching, cultivation pond |
| CN106550910A (en) * | 2016-11-18 | 2017-04-05 | 黄郴 | A kind of Misgurni anguillicaudati artificial breeding method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646763A (en) * | 2017-11-10 | 2018-02-02 | 中国水产科学研究院南海水产研究所 | A kind of macula lutea basket fish artificial fecundation method |
| CN111149735A (en) * | 2020-01-21 | 2020-05-15 | 中国水产科学研究院南海水产研究所深圳试验基地 | Large-scale cultivation method of siganus oramin fry |
| CN111149735B (en) * | 2020-01-21 | 2021-07-27 | 中国水产科学研究院南海水产研究所深圳试验基地 | Large-scale cultivation method of siganus oramin fry |
| CN113647347A (en) * | 2021-09-17 | 2021-11-16 | 广东海洋大学 | Early cultivation method of marine fish seedlings |
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Application publication date: 20170714 |