CN106929453B - Biological agent for treating monosodium glutamate fermentation mother liquor and preparation method thereof - Google Patents
Biological agent for treating monosodium glutamate fermentation mother liquor and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
Abstract
The invention belongs to the technical field of microorganisms, and discloses a biological preparation for treating monosodium glutamate fermentation mother liquor, which is prepared by respectively culturing xylose oxidizing bacillus, micrococcus luteus, nocardia corallina and cellulomonas according to the conventional culture concentration of 1 × 108cuf/g of bacterial liquid, and mixing the four bacterial liquids according to the volume ratio of 5-7:3-5:2-3:2-3 to obtain a liquid bacterial agent; and (3) stirring and mixing the liquid microbial inoculum and the carrier according to the mass ratio of 2-3:3-5, and then drying and packaging to obtain the microbial inoculum. The biological agent only contains four strains, is reasonably compatible, symbiotic and harmonious, and is not antagonistic, long in service life and low in sludge yield.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a biological agent for treating monosodium glutamate fermentation mother liquor and a preparation method thereof.
Background
Monosodium glutamate is a widely used food freshener, a large amount of high-concentration waste liquid is generated in monosodium glutamate production by a fermentation method, COD (chemical oxygen demand) of the monosodium glutamate waste liquid reaches 20-80g/L, the content of suspended matters, sulfate radicals and ammonia nitrogen is high, the pH value is low, the treatment difficulty is high, and the treatment cost is high, so that many monosodium glutamate manufacturers cannot effectively treat the monosodium glutamate waste liquid, great harm is brought to the environment and social development, the pollution problem of the waste liquid in monosodium glutamate industrial production to the environment is serious day by day, and the treatment of the monosodium glutamate waste liquid becomes a great problem restricting the development of monosodium glutamate manufacturers. The main sources of the monosodium glutamate waste liquid are as follows: waste mother liquor or ion exchange tail liquor after glutamic acid is extracted from fermentation liquor; washing waste liquid of various devices (a pulp mixing tank, a liquefaction tank, a saccharification tank, a fermentation tank, an extraction tank, a neutralization and decoloration tank and the like) in the production process; washing and regenerating waste liquid by using ion exchange resin; cooling water for each stage of liquefaction to saccharification, saccharification to fermentation, and the like; various condensed waters (liquefaction, saccharification, concentration, etc.).
The existing fermentation waste liquid treatment methods mainly comprise physical methods, chemical methods and biological methods. The biological treatment method is an effective method for treating industrial polluted water. It is to decompose the compound by the action of microorganism. The biological treatment method has much lower cost than physical and chemical methods, no secondary pollution and stronger variability and adaptability of microorganisms, so the method becomes a more ideal method. The invention discloses a biological agent for treating monosodium glutamate wastewater in the prior patent technology of an applicant, which uses a microbial agent to treat wastewater, has good removal effects on COD, ammonia nitrogen and SS, but has the defects of excessive strains in the microbial agent, increased possibility of strain pollution, relatively difficult culture, large usage amount of the microbial agent, addition of 50g of the microbial agent per ton of sewage, easy deposition of the microbial agent to the bottom of a tank, flocculation caused by a large amount of sludge, and difficult cleaning.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a biological agent for treating monosodium glutamate fermentation mother liquor. The invention also provides a preparation method of the biological preparation.
The invention is realized by the following technical scheme:
the biological preparation for treating monosodium glutamate fermentation mother liquor is prepared by culturing xylose oxidizing bacillus, micrococcus luteus, Nakayama corallinum and Cellulomonas cellulosae respectively at conventional culture concentration of 1 × 108cuf/g of bacterial liquid, and mixing the four bacterial liquids according to the volume ratio of 5-7:3-5:2-3:2-3 to obtain a liquid bacterial agent; and (3) stirring and mixing the liquid microbial inoculum and the carrier according to the mass ratio of 2-3:3-5, and then drying and packaging to obtain the microbial inoculum.
Preferably, the bacterium xyloxygen is Achromobacter xylosoxidans (Achromobacter xylosoxidans. Denitrificas) ATCC 15173.
Preferably, the Micrococcus luteus is Micrococcus luteus (Micrococcus luteus) ATCC 49442.
Preferably, the Nocardia corallina is Nocardia corallina (Nocardia coralline) ACCC 40100.
Preferably, the Cellulomonas is Cellulomonas (Cellulomonas sp) CGMCC No. 2788.
Preferably, the carrier is prepared according to the following process: putting zeolite and kaolinite into a pulverizer according to the mass ratio of 2-3:1-2 for pulverizing, and then grinding into powder of 100 meshes; putting the powder, starch and chitosan into a stirrer according to the mass ratio of 3-5:2-3:1-2, stirring for 10min at 1000rpm to obtain a mixed material, adding the mixed material and polystyrene microspheres into a granulator according to the mass ratio of 1:1, and adding a 6wt% polyvinyl alcohol aqueous solution accounting for 30% of the mass of the mixed material to prepare particles with the particle size of 1-2 mm; drying the particles in an oven at 80 deg.C for 30min, sintering in a sintering furnace at 700 deg.C for 20min, taking out, and naturally cooling to room temperature.
The invention also discloses a preparation method of the biological agent.
The strain of the invention belongs to known strains, and can be purchased from databases of CGMCC, ATCC, ACCC and the like. The scale-up culture of the strains of the invention is a routine culture method in the field, is not an innovative point of the invention and is not detailed here.
The beneficial effects achieved by the invention mainly comprise but are not limited to the following aspects:
the biological agent is obtained by granulating the carrier and the bacterial liquid, has large specific surface area, strong adhesion of thalli and density equivalent to that of a water body, can be suspended in the water body, avoids the influence on decontamination effect caused by uneven distribution of microorganisms due to overlarge density of the agent and precipitation at the bottom of a pool, can also reduce the yield of sludge, and is beneficial to removing pollutants such as COD (chemical oxygen demand), ammonia nitrogen and the like in waste liquid; in order to reduce the dependence on a single specific microbial inoculum and avoid the loss caused by microbial inoculum pollution, the applicant develops various biological agents which complement each other to ensure the normal operation of sewage treatment; the biological preparation only contains four strains, is reasonably compatible, symbiotic and harmonious, does not antagonize each other, reduces the cost and has relatively simple operation process.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the present application will be clearly and completely described below with reference to specific embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The biological preparation for treating the monosodium glutamate fermentation mother liquor is prepared by the following method:
bacillus xylosoxidans (Achromobacter xylosoxidans subsp. Denitrificans) ATCC15173 and Micrococcus luteus (Micrococcus luteus) ATCC 49442. Nocardia corallina (Nocardiaoraline) ACCC40100 and Cellulomonas Cellulomonas (Cellulomonas sp) CGMCC No.2788 are respectively cultured according to the conventional culture concentration of 1 × 108cuf/g of bacterial liquid, and mixing the four bacterial liquids according to the volume ratio of 5:3:3:3 to obtain a liquid bacterial agent; and (3) stirring and mixing the liquid microbial inoculum and the carrier according to the mass ratio of 2:3, then drying at the drying temperature of 20 ℃, and packaging after drying, thus obtaining the microbial inoculum.
The carrier is prepared according to the following process:
putting zeolite and kaolinite into a pulverizer according to the mass ratio of 2:1 for pulverizing, and then grinding into powder of 100 meshes; putting the powder, starch and chitosan into a stirrer according to the mass ratio of 5:3:2, stirring at 1000rpm for 10min to obtain a mixed material, adding the mixed material and polystyrene microspheres into a granulator according to the mass ratio of 1:1, and adding a polyvinyl alcohol aqueous solution with the concentration of 6wt% accounting for 30% of the mass of the mixed material to prepare granules with the particle size of 2 mm; drying the particles in an oven at 80 deg.C for 30min, sintering in a sintering furnace at 700 deg.C for 20min, taking out, and naturally cooling to room temperature.
Example 2
The biological preparation for treating the monosodium glutamate fermentation mother liquor is prepared by the following method:
bacillus xylosoxidans (Achromobacter xylosoxidans subsp. densitificans) ATCC15173, Micrococcus luteus (ATCC 49442), Nocardia corallina (Nocardia coralline) ACCC40100, Cellulomonas Cellulomonas (Cellulomonas sp) CGMCC No.2788 were cultured at a concentration of 1 × 108cuf/g of bacterial liquid, and mixing the four bacterial liquids according to the volume ratio of 5:3:3:2 to obtain a liquid bacterial agent; and (3) stirring and mixing the liquid microbial inoculum and the carrier according to the mass ratio of 3:5, then drying at the drying temperature of 20 ℃, and packaging after drying, thus obtaining the microbial inoculum.
The carrier is prepared according to the following process:
putting zeolite and kaolinite into a pulverizer according to the mass ratio of 3:2 for pulverizing, and then grinding into powder of 100 meshes; putting the powder, starch and chitosan into a stirrer according to the mass ratio of 3:2:1, stirring at 1000rpm for 10min to obtain a mixed material, adding the mixed material and polystyrene microspheres into a granulator according to the mass ratio of 1:1, and adding a polyvinyl alcohol aqueous solution with the concentration of 6wt% accounting for 30% of the mass of the mixed material to prepare particles with the particle size of 1 mm; drying the particles in an oven at 80 deg.C for 30min, sintering in a sintering furnace at 700 deg.C for 20min, taking out, and naturally cooling to room temperature.
Example 3
The biological preparation for treating the monosodium glutamate fermentation mother liquor is prepared by the following method:
bacillus xylosoxidans (Achromobacter xylosoxidans subsp. densitificans) ATCC15173, Micrococcus luteus (ATCC 49442), Nocardia corallina (Nocardia coralline) ACCC40100, Cellulomonas Cellulomonas (Cellulomonas sp) CGMCC No.2788 were cultured at a concentration of 1 × 108cuf/g of bacterial liquid, and mixing the four bacterial liquids according to the volume ratio of 5:5:3:3 to obtain a liquid bacterial agent; stirring and mixing the liquid microbial inoculum and the carrier according to the mass ratio of 1:1, then drying at the drying temperature of 20 ℃, and packaging after drying, wherein the water content is 125%.
The carrier is prepared according to the following process:
putting zeolite and kaolinite into a pulverizer according to the mass ratio of 1:1 for pulverizing, and then grinding into powder of 100 meshes; putting the powder, starch and chitosan into a stirrer according to the mass ratio of 3:2:2, stirring at 1000rpm for 10min to obtain a mixed material, adding the mixed material and polystyrene microspheres into a granulator according to the mass ratio of 1:1, and adding a polyvinyl alcohol aqueous solution with the concentration of 6wt% accounting for 30% of the mass of the mixed material to prepare particles with the particle size of 1 mm; drying the particles in an oven at 80 deg.C for 30min, sintering in a sintering furnace at 700 deg.C for 20min, taking out, and naturally cooling to room temperature.
Example 4
The biological agent of the invention has the following effects:
taking monosodium glutamate fermentation waste mother liquor (COD is 954mg/L, ammonia nitrogen is 119mg/L, and sulfide is 37 mg/L) which is treated by the working procedures of precipitation and the like in a production workshop of the inner mons monster;
the treatment process comprises the following steps: adding 2 g of microbial preparation into each cubic meter of liquid every time, adding for 1 time every day, continuously adding for 5 days, standing for 3 days, and finally filtering and discharging.
Sampling and measuring COD, ammonia nitrogen and sulfide data; and a control group is set, and the compatibility effect of each strain in the microbial inoculum is detected: control group 1: the same procedure as in example 1 was repeated except that no Xylobacillus xylosoxidans was added; control group 2: the procedure of example 1 was repeated except that Micrococcus luteus was not added; control group 3: the same procedure as in example 1 was repeated except that no Pseudocercospora corallina was added; control group 4: the procedure of example 1 was repeated except that Cellulomonas was not added. Specific detection results are shown in table 1:
TABLE 1
| Example 1 (mg/L) | Control group 1 (mg/L) | Control group 2 (mg/L) | Control group 3 (mg/L) | Control group 4 (mg/L) | |
| COD | 5.8 | 30.6 | 66.4 | 41.6 | 59.8 |
| NH3-N | 2.1 | 8.1 | 13.7 | 9.4 | 6.1 |
| Sulfide compound | 1.2 | 2.9 | 5.9 | 4.1 | 3.7 |
And (4) conclusion: the biological preparation provided by the invention has the advantages of reasonable compatibility of fungi, strong cooperativity and small usage amount, and can effectively remove COD, ammonia nitrogen and sulfides in fermentation waste liquid.
Example 5
Test groups: the biological preparation prepared in example 1 of the present invention;
control group: the applicant's prior invention patent technology "a biological agent for treating monosodium glutamate wastewater" the biological agent prepared in example 1.
Indexes such as density and sludge production of the biological agent are detected, and the indexes are specifically shown in table 2:
TABLE 2
| Index (I) | Density g/ml | 10 th sludge production amount g/L | The 20 th sludge production amount is g/L |
| Test group | 1.03 | 3.2 | 9.8 |
| Control group | 1.36 | 8.1 | 21.6 |
And (4) conclusion: the biological agent has the same density as liquid, can be suspended in the liquid, avoids depositing and flocculating at the bottom of the tank to generate a large amount of sludge, has higher hardness, is not easy to break and has long service life.
Finally, it is also noted that the above-mentioned lists merely illustrate a few specific embodiments of the invention. It is obvious that the invention is not limited to the above embodiments, but that many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.
Claims (2)
1. The biological preparation for treating the monosodium glutamate fermentation mother liquor is prepared by the following method:
respectively culturing Xylobacillus xylosoxidans, Micrococcus luteus, Nocardia coralline and Cellulomonas cellulosae at conventional culture concentration of 1 × 108cuf/g of bacterial liquid, and mixing the four bacterial liquids according to the volume ratio of 5-7:3-5:2-3:2-3 to obtain a liquid bacterial agent; stirring and mixing the liquid microbial inoculum and the carrier according to the mass ratio of 2-3:3-5, then drying and packaging to obtain the microbial inoculum;
said xylose oxidizing bacterium is xylose oxidizing bacterium (Achromobacter xylosoxidansubs. dentifrices) ATCC 15173;
the Micrococcus luteus is Micrococcus luteus (Micrococcus luteus) ATCC 49442;
the Nocardia corallina is Nocardia corallina (Nocardia coralline) ACCC 40100;
the Cellulomonas is Cellulomonas sp (CGMCC No. 2788).
2. The biological agent according to claim 1, wherein the carrier is prepared by the following process: putting zeolite and kaolinite into a pulverizer according to the mass ratio of 2-3:1-2 for pulverizing, and then grinding into powder of 100 meshes; putting the powder, starch and chitosan into a stirrer according to the mass ratio of 3-5:2-3:1-2, stirring for 10min at 1000rpm to obtain a mixed material, adding the mixed material and polystyrene microspheres into a granulator according to the mass ratio of 1:1, and adding a 6wt% polyvinyl alcohol aqueous solution accounting for 30% of the mass of the mixed material to prepare particles with the particle size of 1-2 mm; drying the particles in an oven at 80 deg.C for 30min, sintering in a sintering furnace at 700 deg.C for 20min, taking out, and naturally cooling to room temperature.
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| CN110499262A (en) * | 2018-05-17 | 2019-11-26 | 卢松 | Purification absorbs the compound of slurry-spraying pelletizing flue gas |
| CN110102177B (en) * | 2019-04-09 | 2021-11-26 | 内蒙古阜丰生物科技有限公司 | Process for purifying flue gas generated in guniting granulation |
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