CN106916755A - A kind of culture medium of suitable white-rot fungi growth - Google Patents
A kind of culture medium of suitable white-rot fungi growth Download PDFInfo
- Publication number
- CN106916755A CN106916755A CN201710224901.2A CN201710224901A CN106916755A CN 106916755 A CN106916755 A CN 106916755A CN 201710224901 A CN201710224901 A CN 201710224901A CN 106916755 A CN106916755 A CN 106916755A
- Authority
- CN
- China
- Prior art keywords
- white
- rot fungi
- culture medium
- culture
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of culture medium.A kind of culture medium of suitable white-rot fungi growth, culture medium per 1000mL contains the yeast extract of 2.4~3.6g, the peptone of 4~6g, the maltose of 8~12g, the glucose of 8~12g, 0.8~1.2g dipotassium hydrogen phosphates, the magnesium sulfate of 0.4~0.6g, the vitamin B of 0.0016~0.0024g1, 0.0008~0.0012g biotin and the agar of 16~24g.The white-rot fungi growth rate of medium culture of the invention is existing culture medium more than 1.16 times.The growth rate for solving the white-rot fungi of existing medium culture is slow, it is impossible to meet in large-scale application white-rot fungi, to the problem of white-rot fungi wilderness demand.
Description
Technical field
The present invention relates to a kind of culture medium.
Background technology
With people's environmental consciousness continuous enhancing and domestic and international environmental regulation it is increasingly strict, modern biotechnology is wide
General is applied in the production of green industry.White-rot fungi as a kind of fungi that can secrete lignin degrading enzyme system, in environment
The fields such as reparation, degradation of contaminant, food industry, paper industry have a good application prospect.Under the conditions of optimum, use
Existing medium culture white-rot fungi, the growth rate of the white-rot fungi of culture is only 6mm/d, it is impossible to meets and on a large scale should
When using white-rot fungi, to the problem of white-rot fungi wilderness demand.
The content of the invention
The technical problems to be solved by the invention are：How the growth rate of the white-rot fungi of existing medium culture is solved
Slow problem.
The technical solution adopted in the present invention is：A kind of culture medium of suitable white-rot fungi growth, the culture per 1000mL
Yeast extract containing 2.4~3.6g, the peptone of 4~6g, the maltose of 8~12g, the glucose of 8~12g, 0.8 in base
~1.2g dipotassium hydrogen phosphates, the magnesium sulfate of 0.4~0.6g, the vitamin B of 0.0016~0.0024g1, 0.0008~
The biotin of 0.0012g, the agar of 16~24g, remaining is water.The dipotassium hydrogen phosphate added in the technical program can not only be carried
For the potassium element of growth needs, and culture medium can be made to be in the environment of weak acid partial neutral, the growth of suitable white-rot fungi.
The beneficial effects of the invention are as follows：The growth rate of the white-rot fungi of medium culture of the invention is 7mm/d or so,
It is existing culture medium more than 1.16 times, is conducive to the growth of white-rot fungi.
Specific embodiment
Embodiment one：
Yeast extract, the peptone of 6g, the maltose of 8g, the glucose of 12g, the 0.8g phosphorus of 3.6g are added in volumetric flask
Sour hydrogen dipotassium, the magnesium sulfate of 0.4g, the vitamin B of 0.0024g1, 0.0012g biotins and 16g agar, then add water
And stir, solution total amount is 1000mL in making volumetric flask, then pours into high-temperature sterilization vial in 121 solutionoC high temperature
Sterilizing 15 minutes is simultaneously cooled to 50-60 oC, then proceed to stir and pour into form uniform soluble solids in aseptic culture dish.
Embodiment two：
Yeast extract, the peptone of 5g, the maltose of 10g, the glucose of 10g, the 1g phosphoric acid hydrogen two of 3g are added in volumetric flask
Potassium, the magnesium sulfate of 0. 5g, the vitamin B of 0.002g1, the biotin of 0.001g, the agar of 20g, addition water simultaneously stir, make appearance
Solution total amount is 1000mL in measuring bottle, then pours into high-temperature sterilization vial in 121 solutionoC high-temperature sterilizations 15 minutes
And it is cooled to 50-60 oC, then proceed to stir and pour into form uniform soluble solids in aseptic culture dish.
By the culture medium in embodiment one and embodiment two, (culture medium per 1000mL contains with existing culture medium：20g's
The potato leachate of glucose, the agar of 20g and 200mL) all for cultivating Trametes versicolor, (Tramates discolor are white
One kind of rotten fungi), and carry out contrast experiment.
Experimental procedure is as follows：The existing culture medium that will cover with white-rot fungi with aseptic card punch on aseptic operating platform breaks into
The bacterium piece of 8mm, is inoculated into the culture medium of the present embodiment containing 30mL one and embodiment two and the culture dish of existing culture medium respectively
In；Cultivated under conditions of 30 DEG C of incubator, a hyphal diameter is determined daily, determined 7 days, then measure white rot in culture medium
The growth rate of fungi.
Test result indicate that：The growth rate of the white-rot fungi in embodiment one is 7.1mm/d, the white rot in embodiment two
The growth rate of fungi is 7mm/d, and the growth rate of the white-rot fungi of existing medium culture is 6mm/d, culture of the invention
The growth rate of the white-rot fungi of base culture is the growth rate of the white-rot fungi of existing medium culture more than 1.16 times.
Claims (1)
1. the culture medium that a kind of suitable white-rot fungi grows, it is characterised in that：Culture medium kind per 1000mL contains 2.4~3.6g
Yeast extract, the peptone of 4~6g, the maltose of 8~12g, the glucose of 8~12g, 0.8~1.2g dipotassium hydrogen phosphates,
The magnesium sulfate of 0.4~0.6g, the vitamin B of 0.0016~0.0024g1, the biotin of 0.0008~0.0012g, 16~24g
Agar, remaining is water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710224901.2A CN106916755A (en) | 2017-04-07 | 2017-04-07 | A kind of culture medium of suitable white-rot fungi growth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710224901.2A CN106916755A (en) | 2017-04-07 | 2017-04-07 | A kind of culture medium of suitable white-rot fungi growth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106916755A true CN106916755A (en) | 2017-07-04 |
Family
ID=59568508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710224901.2A Pending CN106916755A (en) | 2017-04-07 | 2017-04-07 | A kind of culture medium of suitable white-rot fungi growth |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106916755A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100430961B1 (en) * | 2002-02-26 | 2004-05-12 | 김진호 | Serratia plymuthica antagonistic to white rot fungi sclerotium cepivorum |
| JP2005304424A (en) * | 2004-04-23 | 2005-11-04 | Taisei Corp | Medium for white-rot fungus and method for culturing white-rot fungus |
| CN101260389A (en) * | 2008-04-15 | 2008-09-10 | 华东师范大学 | Enzyme producing method for white rot fungus |
| CN102399704A (en) * | 2011-11-29 | 2012-04-04 | 华南农业大学 | Liquid culture medium suitable for growth of white rot fungi and capable of producing laccase and application of liquid culture medium |
-
2017
- 2017-04-07 CN CN201710224901.2A patent/CN106916755A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100430961B1 (en) * | 2002-02-26 | 2004-05-12 | 김진호 | Serratia plymuthica antagonistic to white rot fungi sclerotium cepivorum |
| JP2005304424A (en) * | 2004-04-23 | 2005-11-04 | Taisei Corp | Medium for white-rot fungus and method for culturing white-rot fungus |
| CN101260389A (en) * | 2008-04-15 | 2008-09-10 | 华东师范大学 | Enzyme producing method for white rot fungus |
| CN102399704A (en) * | 2011-11-29 | 2012-04-04 | 华南农业大学 | Liquid culture medium suitable for growth of white rot fungi and capable of producing laccase and application of liquid culture medium |
Non-Patent Citations (4)
| Title |
|---|
| 严平等: "高效木质素降解菌的复合诱变选育", 《湖北农业科学》 * |
| 吴 薇等: "高效木质素降解菌的分离筛选", 《食品开发与机械》 * |
| 许庆国等: "云芝培养基配方筛选", 《山东农业科学》 * |
| 赵文娟等: "变色栓菌产漆酶培养条件优化及酶学特性研究", 《食品工业》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101669559B (en) | Production method for improving quality of Liupu tea | |
| CN105559088B (en) | A kind of weight-reducing ferment and preparation method thereof | |
| CN103820340B (en) | A kind of yeast rich in selenium and preparation method thereof | |
| CN104328056B (en) | Trichoderma reesei capable of producing cellulase in high yield and application of trichoderma reesei | |
| CN102210449B (en) | Liquid submerged fermentation culture of Thelephora ganbajun Zang and preparation methods of seasoning and selenium-enriched product of Thelephora ganbajun Zan | |
| CN106434369B (en) | One plant of aspergillus oryzae for producing L MALIC ACID and its application | |
| CN107488640A (en) | A kind of resistance to oxidation low temperature glucose oxidase and its production method and application | |
| CN101407800B (en) | Neuter protease produced by using Paenibacillus polymyxa from sea and method thereof | |
| CN106119131A (en) | A kind of method alleviating excess copper suppression Growth of Pleurotus Mycelium | |
| CN102643729A (en) | Culture method of applying bacillus licheniformis to sesame-flavor bacterial mouldy bran | |
| CN102851259B (en) | Preparation method of antioxidant | |
| CN108095129A (en) | A kind of method that fermentation prepares wheat bran water-soluble dietary fiber | |
| CN104130950A (en) | Aspergillus niger and cultivation method and application thereof | |
| CN105255807B (en) | A kind of conidial preparation method of coronoid process dissipate capsule bacterium | |
| CN104762229B (en) | A kind of bacillus subtilis strain and its application | |
| CN106520575B (en) | One plant height produces Aspergillus niger strain and its application of catalase | |
| CN106916755A (en) | A kind of culture medium of suitable white-rot fungi growth | |
| CN109906268A (en) | For producing the fermentation process of D-ALPHA-Hydroxypropionic acid or its salt | |
| CN104059857A (en) | Aspergillus and application of aspergillus in fructosyltransferase preparing | |
| CN106282145B (en) | A kind of liquid state fermentation method of adenylic acid deaminase | |
| CN106318999A (en) | Method for preparing pumpkin seed meal molecular peptide through solid state fermentation on pumpkin seed cake meal by virtue of mixed bacteria | |
| CN105861574B (en) | A method of it is fermented using aspergillus oryzae and produces L MALIC ACID | |
| CN101736034A (en) | Method for preparing low-citrinin monascus pigment through submerged fermentation | |
| CN109777709A (en) | A kind of liquid fermented tea novel fermentation vinegar technique | |
| CN104711208B (en) | A kind of lactic acid bacteria with high starch capacity of decomposition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170704 |
|
| RJ01 | Rejection of invention patent application after publication |