CN106890202A - Purslane extract solution and preparation method thereof - Google Patents

Purslane extract solution and preparation method thereof Download PDF

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Publication number
CN106890202A
CN106890202A CN201710113976.3A CN201710113976A CN106890202A CN 106890202 A CN106890202 A CN 106890202A CN 201710113976 A CN201710113976 A CN 201710113976A CN 106890202 A CN106890202 A CN 106890202A
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purslane
extract solution
parts
preparation
purslane extract
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Inventor
施春华
张美芬
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Shengzhou City Paitepu Technology Development Co Ltd
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Shengzhou City Paitepu Technology Development Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention provides a kind of purslane extract solution, including following component：Alkaloid 0.2 ~ 0.7%, total flavonoid content is 15.0 ~ 40%, saponin 5 ~ 10%, remaining water, this purslane extract solution that the present invention is provided and preparation method thereof, is prepared simply, and DNA purity is high, the pharmacological action of raising purslane.

Description

Purslane extract solution and preparation method thereof
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, especially a kind of purslane extract solution and preparation method thereof.
Background technology
With the development of society, people gradually step up to the utilization rate of Chinese medicine, and people improve Chinese medicine using various ways Using effect.
For example：Purslane extract and its production and use, CN101181317A, invention is related to medical science to lead Domain, is a kind of extract and extracting method prepared as raw material with Portulaca oleracea L, and the extract is used to prepare anti-lacking Oxygen, antifatigue, resisting stress medicine and food, conventional purslane extract is to take supernatant using after water or alcohol extracting, from The precipitation that the heart is produced is abandoned as invalid components, the invention it has been investigated that after the suitably concentration of Portulaca water or alcohol extract, from The sediment that the heart is produced has significant anti anoxia, antifatigue, anti-stress effect on the contrary.The invention provides a kind of new dent The preparation method of amaranth extract, purslane extract obtained in the method the experiment proved that it has preferable anti anoxia, anti-tired Labor, anti-stress effect.
Invention preparation process in preparation process is relatively easy, and the purslane extract concentration that preparation lifts is relatively low.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of raising to prepare concentration, and recovery rate is high, and purity is high, dent Amaranth extract solution and preparation method thereof.
To solve above-mentioned existing technical problem, the present invention uses following scheme：A kind of purslane extract solution, including Following component：Alkaloid 0.2- 0.7%, saponin 5-10%, general flavone 15-40%, vitamin 4-8%, remaining water.
1）Purslane fresh grass is taken, sieving 150-200 mesh is dry, pulverize after cleaning, obtain purslane powder；
2）Extractant is added in purslane powder, is soaked, stirring, ultrasonic wave hydrotropy, filtering merges extract solution；
3）Aqueous sulfuric acid regulation pH value and 2.2 ~ 4h of back hydrolysis, filtering, ultrafiltration, dialysis are added in extract solution；
4）Decolorising agent, decolouring is added to be filtered after terminating in stagnant liquid of dialysing, concentration obtains purslane extract solution.
Preferably, dry mode freeze-drying in step 1, purslane fresh grass is placed in a low temperature of -13 ~ -21 DEG C, is taken out Vacuum ensures the freshness of purslane fresh grass using freeze-drying to 4 ~ 6 handkerchief freeze-dryings, keeps the chemical group of purslane fresh grass Knit and physical property.
Preferably, the extractant of addition and the weight ratio of purslane powder are 3 ~ 8：1, soak time be 1 ~ 2h, and stir 10 ~ 15min, the composition and its weight portion of the extractant are：Water 5-60 parts, cellulase 0.5-3.8 parts, pectase 0-3 parts, gold 0.1-3 parts of chelating agent of category, glucose 2-8 parts, organic acid 0.2-25 parts, dihydropyridine 0.02-0.05 parts, diborane 0.01- 0.04 part, ethanol 45-75 parts, repeat to extract 1 ~ 3 time.
Preferably, the concentration of aqueous sulfuric acid is 2-4%, ph values regulation to 4.8-5.2.
Preferably, milipore filter is 1500-3000Da in step 3, and dialysis membrane is 100 ~ 300Da, can be by big molecular impurity such as Protein, polysaccharide removal, reach the purpose for isolating and purifying.
Preferably, decolorising agent is in step 4：Activated carbon or diatomite, except impurity such as depigmentatons, improve purslane extract Purity.
Preferably, it is stirred in decolorization, stir speed (S.S.) is 100 ~ 200r/min, bleaching time is 2.0 ~ 3.0h.
Preferably, soak time is 1 ~ 2h in step 2, and stir 10 ~ 15min, the composition and its weight portion of extractant For：Water 5-60 parts, cellulase 0.5-3.8 parts, pectase 0-3 parts, metal-chelator 0.1-3 parts, glucose 2-8 parts, it is organic It is sour 0.2-25 parts, dihydropyridine 0.02-0.05 parts, diborane 0.01-0.04 parts, ethanol 45-75 parts, the extractant for using can have The cell membrane of effect destruction purslane, is dissolved into extractant beneficial to cellular content, coordinates ultrasonic wave hydrotropy, fully extracts dent Benefit materials in amaranth, improve the recovery rate and purity of purslane benefit materials.
Beneficial effect：This purslane extract solution that the present invention is provided and preparation method thereof, prepares simply, extracts pure Degree is high, improves the pharmacological action of purslane.
A kind of purslane extract solution and preparation method thereof is provided present invention employs above-mentioned technical proposal, be compensate for existing There is the deficiency of technology, it is reasonable in design, it is easy to operate.
Specific embodiment
Following examples are described in further detail：
Embodiment 1：
A kind of purslane extract solution, including following component：Alkaloid 0.2- 0.7%, saponin 5-10%, general flavone 15- 40%, vitamin 4-8%, remaining water.
1）Purslane fresh grass is taken, sieving 150-200 mesh is dry, pulverize after cleaning, obtain purslane powder；
2）Extractant is added in purslane powder, is soaked, stirring, ultrasonic wave hydrotropy, filtering merges extract solution；
3）Aqueous sulfuric acid regulation pH value and 2.2 ~ 4h of back hydrolysis, filtering, ultrafiltration, dialysis are added in extract solution；
4）Decolorising agent, decolouring is added to be filtered after terminating in stagnant liquid of dialysing, concentration obtains purslane extract solution.
Dry mode freeze-drying, purslane fresh grass is placed in a low temperature of -13 ~ -21 DEG C in step 1, is evacuated to 4 ~ 6 handkerchief freeze-dryings, the freshness of purslane fresh grass is ensured using freeze-drying, keeps the chemistry tissue and thing of purslane fresh grass Rationality matter.
The extractant of addition is 3 ~ 8 with the weight ratio of purslane powder：1, soak time is 1 ~ 2h, and stirs 10 ~ 15min, The composition and its weight portion of the extractant be：Water 5-60 parts, cellulase 0.5-3.8 parts, pectase 0-3 parts, metal-chelating Agent 0.1-3 parts, glucose 2-8 parts, organic acid 0.2-25 parts, dihydropyridine 0.02-0.05 parts, diborane 0.01-0.04 parts, second Alcohol 45-75 parts, repeat to extract 1 ~ 3 time.
The concentration of aqueous sulfuric acid is 2-4%, ph values regulation to 4.8-5.2.
In step 3 milipore filter be 1500-3000Da, dialysis membrane be 100 ~ 300Da, can by big molecular impurity such as protein, Polysaccharide is removed, and reaches the purpose for isolating and purifying.
Decolorising agent is in step 4：Activated carbon or diatomite, except impurity such as depigmentatons, improve the purity of purslane extract.
It is stirred in decolorization, stir speed (S.S.) is 100 ~ 200r/min, bleaching time is 2.0 ~ 3.0h.
Soak time is 1 ~ 2h in step 2, and stirs 10 ~ 15min, and the composition and its weight portion of extractant are：Water 5-60 Part, cellulase 0.5-3.8 parts, pectase 0-3 parts, metal-chelator 0.1-3 parts, glucose 2-8 parts, organic acid 0.2-25 Part, dihydropyridine 0.02-0.05 parts, diborane 0.01-0.04 parts, ethanol 45-75 parts, the extractant for using can effectively destroy horse The cell membrane of bitterroot, is dissolved into extractant beneficial to cellular content, coordinates ultrasonic wave hydrotropy, fully having in extraction purslane Beneficial material, improves the recovery rate and purity of purslane benefit materials.
Embodiment 2：
A kind of purslane extract, purslane extract solution is prepared through purslane extract preparation method of the invention, right Purslane extract solution carries out pharmacological evaluation：
Choose 20~25g of body weight health Kunming mouses, random point of 5 groups, every group 10, male and female half and half.The purslane of preparation carries Take thing solution and 1g/ml, 2g/ml, 4g/ml suspension are formulated as with 0.5%CMC-Na respectively, ginseng stem and leave general saponin is with 0.5% CMC-Na is formulated as 10mg/ml suspensions.Each group animal continuous gavage is administered one week, and administered volume 0.5ml, blank control group is given Give isometric blank supensoid agent.Each group animal in being put into after last dose in the 200ml wide-mouth bottles for filling 10g soda limes, every bottle 1 Only, bottleneck is smeared with vaseline, covers tightly and be allowed to air tight, external application preservative film seals bottleneck again, obturaged timing at once from bottle cap, Stop being index to breathe, record mouse asphyxiating dead time because of anoxic.The results are shown in Table 1.Result shows that purslane carries Taking the basic, normal, high dosage group of thing all has significant oxygen lack resistant function.
The purslane extract resist oxygen lack experimental result of table 1
Group Size of animal Dosage Hypoxia endurance time min
Blank control group 10 - 44.6±6.2
Ginseng stem and leave general saponin group 10 12mg/ is only 58.3±8.2
Purslane extract low dose group 10 0.6g/ is only 55.7±7.6
Purslane extract middle dose group 10 1.2g/ is only 58.8±9.7
Purslane extract high dose group 10 2g/ is only 65.4±12.3
Ibid, be placed in for each group mouse (single to be only put into mouse cage) respectively low by 1h after last dose for experiment packet and medication (- 22 DEG C are adjusted in temperature refrigerator), the time-to-live of observed and recorded each group mouse.The results are shown in Table 2：
The purslane extract low-temperature resistance stress experimental result of table 2
Group Size of animal Dosage Time-to-live min
Blank control group 10 - 67.2±5.2
Ginseng stem and leave general saponin group 10 12mg/ is only 78.6±7.4
Purslane extract low dose group 10 0.6g/ is only 70.4±4.6
Purslane extract middle dose group 10 1.2g/ is only 83.5±7.1
Purslane extract high dose group 10 2g/ is only 77.4±8.3
Result shows that the basic, normal, high dosage group of purslane extract is all acted on significant low-temperature resistance stress.
Specific embodiment described herein be only to explanation for example of the invention, the technical field of the invention Technical staff can be made various modifications or supplement to described specific embodiment or be substituted using similar mode, but Without departing from of the invention or surmount scope defined in appended claims.

Claims (8)

1. a kind of purslane extract solution, it is characterised in that including following component：Alkaloid 0.2- 0.7%, saponin 5- 10%, general flavone 15-40%, vitamin 4-8%, remaining water.
2. a kind of preparation method of purslane extract solution, it is characterised in that comprise the following steps：
1）Purslane fresh grass is taken, sieving 150-200 mesh is dry, pulverize after cleaning, obtain purslane powder；
2）Extractant is added in purslane powder, is soaked, stirring, ultrasonic wave hydrotropy, filtering merges extract solution；
3）Aqueous sulfuric acid regulation pH value and 2.2 ~ 4h of back hydrolysis, filtering, ultrafiltration, dialysis are added in extract solution；
4）Decolorising agent, decolouring is added to be filtered after terminating in stagnant liquid of dialysing, concentration obtains purslane extract solution.
3. the preparation method of a kind of purslane extract solution according to claim 1, it is characterised in that：The step 1 In dry mode freeze-drying, purslane fresh grass is placed in a low temperature of -13 ~ -21 DEG C, be evacuated to 4 ~ 6 handkerchief freeze-dryings.
4. the preparation method of a kind of purslane extract solution according to claim 1, it is characterised in that：The step 2 The extractant of middle addition is 3 ~ 8 with the weight ratio of purslane powder：1, soak time is 1 ~ 2h, and stirs 10 ~ 15min, described to carry The composition and its weight portion for taking agent be：Water 5-60 parts, cellulase 0.5-3.8 parts, pectase 0-3 parts, metal-chelator 0.1-3 Part, glucose 2-8 parts, organic acid 0.2-25 parts, dihydropyridine 0.02-0.05 parts, diborane 0.01-0.04 parts, ethanol 45-75 Part, repeat to extract 1 ~ 3 time.
5. the preparation method of a kind of purslane extract solution according to claim 1, it is characterised in that：The step 3 The concentration of middle aqueous sulfuric acid is 2-4%, ph values regulation to 4.8-5.2.
6. the preparation method of a kind of purslane extract solution according to claim 1, it is characterised in that：The step 3 Middle milipore filter is 1500-3000Da, and dialysis membrane is 100 ~ 300Da.
7. the preparation method of a kind of purslane extract solution according to claim 1, it is characterised in that：The step 4 Middle decolorising agent is：Activated carbon or diatomite.
8. the preparation method of a kind of purslane extract solution according to claim 1, it is characterised in that：The step 4 It is stirred in middle decolorization, stir speed (S.S.) is 100 ~ 200r/min, bleaching time is 2.0 ~ 3.0h.
CN201710113976.3A 2017-02-28 2017-02-28 Purslane extract solution and preparation method thereof Pending CN106890202A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107459403A (en) * 2017-09-22 2017-12-12 芜湖新达园林绿化集团有限公司 A kind of nutrition equilibrium asparagus fern compound fertilizer special
CN109464476A (en) * 2018-11-09 2019-03-15 嘉兴职业技术学院 The purslane flavones Extraction Processes of response phase method optimization
CN117298021A (en) * 2023-11-28 2023-12-29 珠海金肽生物科技有限公司 Composition with anti-inflammatory and soothing effects and preparation method and application thereof

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Publication number Priority date Publication date Assignee Title
US20010031283A1 (en) * 1999-06-17 2001-10-18 Elsie Belcheff Food supplement
CN102389385A (en) * 2011-05-27 2012-03-28 上海辉文生物技术有限公司 Herba portulacae extract and solution, and preparation method and application
CN105596389A (en) * 2016-03-15 2016-05-25 南京塔木德母婴产品有限公司 Device for extracting natural herbal active ingredient of purslane

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010031283A1 (en) * 1999-06-17 2001-10-18 Elsie Belcheff Food supplement
CN102389385A (en) * 2011-05-27 2012-03-28 上海辉文生物技术有限公司 Herba portulacae extract and solution, and preparation method and application
CN105596389A (en) * 2016-03-15 2016-05-25 南京塔木德母婴产品有限公司 Device for extracting natural herbal active ingredient of purslane

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107459403A (en) * 2017-09-22 2017-12-12 芜湖新达园林绿化集团有限公司 A kind of nutrition equilibrium asparagus fern compound fertilizer special
CN109464476A (en) * 2018-11-09 2019-03-15 嘉兴职业技术学院 The purslane flavones Extraction Processes of response phase method optimization
CN117298021A (en) * 2023-11-28 2023-12-29 珠海金肽生物科技有限公司 Composition with anti-inflammatory and soothing effects and preparation method and application thereof
CN117298021B (en) * 2023-11-28 2024-02-06 珠海金肽生物科技有限公司 Composition with anti-inflammatory and soothing effects and preparation method and application thereof

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Application publication date: 20170627