CN106888959A - A kind of selection of loose panicle type perfume rice two-line sterile line - Google Patents
A kind of selection of loose panicle type perfume rice two-line sterile line Download PDFInfo
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- CN106888959A CN106888959A CN201710047683.XA CN201710047683A CN106888959A CN 106888959 A CN106888959 A CN 106888959A CN 201710047683 A CN201710047683 A CN 201710047683A CN 106888959 A CN106888959 A CN 106888959A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selection of loose panicle type perfume rice two-line sterile line, belong to Special rice sterile line breeding technical field.The present invention is with two-line sterile line of rice（One is dual-purpose）, loose panicle type rice strain, fragrant rice strain be hybrid strain；Using the three hybridization method for group matching handed over, planted by under two-line sterile line infertility phase and the different light and temperature conditions that the phase can be educated, and identified through the fringe type in many generations, fragrance, sterility and fertility, Comprehensive Traits are screened, be bred as that loose panicle type, rice aroma are strong, sterility is stable, coordinate force good, breeding, the production of hybrid seeds and the fragrant rice two-line sterile line of hybrid yield excellent loose panicle high.The sterile line prepare hybrid seed outcrossing seed-setting rate is high, seed procreation ability is strong, rice outward appearance and Cooking Quality are good, and the fringe portion pest and disease damage such as false smut substantially mitigates, and can reduce the usage amount of agricultural chemicals, increase farmers' income.
Description
Technical field
The invention belongs to Special rice sterile line breeding technical field, and in particular to a kind of loose panicle type perfume rice two-line sterile line
Selection.
Background technology
It is to realize one of important channel that rice yield is broken through, fringe type problem that ideotype is combined with heterosis utilization
The focus that always Breeding Rice for Ideotype and cultivation are studied, fringe type has with rice yield level and group structure situation
Close relationship.The angle of branching pattern, Primary branch and the dense degree of small ear and spatial attitude according to fringe, can be by fringe
Type is divided into intensity（Compact panicle）, scatter type（Loose panicle type）（Spreading panicle）And osculant
（Intermediate panicle）.So far, 7 loose panicle character genes are reported altogether, wherein,spr1Withspr8By 1
Recessive nuclear gene is controlled, and remaining 5 loose panicle character gene is controlled by dominant gene.Research shows that rice panicle type is loosely favourable
In the procreation ability for improving its outcrossing seed-setting rate and seed, fringe type scatters and is conducive to increasing the space between fringe portion Primary branch,
The increase of fringe portion branch stalk spreading angle, increased that illumination is penetrating and grain filling characteristics, reduce the chalky grain rate of rice and total
Chalkiness degree, blue or green flat seed is significantly reduced, and improves exterior quality.Meanwhile, loose panicle type branch obstructs that the male character types in Chinese operas, usu. referring tov the bearded character degree is big, fringe shape is relatively loose
Dissipate, improve the pest and disease damages such as fringe portion miniclimate, fringe portion false smut and substantially mitigate.Seed selection loose panicle type two-line sterile line of rice can match somebody with somebody
Group goes out the new hybrid rice varieties of high yield and high quality.
Scented rice rice is a kind of high-quality seed rice with tempting special fragrance.Its type includes that perfume Xian, fragrant round-grained rice and perfume (or spice) are glutinous, has
There is special fragrance, the grain of rice is sparkling and crystal-clear, rich in the various necessary nutritional ingredients of human body, have certain nourishing and medicinal effects.Its fragrance
Can be disappeared removing fatigue, and help to improve a poor appetite.In addition to it can make rice, the food such as various rice wine, rice cake can be also produced, its taste is more
It is good.Some aromatic rices（System）It is applied to after improvement in production, has obtained preferable economic benefit, some kinds has started to away
To international market.But domestic these fragrant rice strain fringe types being bred as are intensity at present, there is no loose panicle type perfume rice two is infertility
The report of system.
This method chooses two-line sterile line of rice（One is dual-purpose）, loose panicle type rice strain, fragrant rice strain, eary maturity of rice is extensive
Multiple system, ripe restorer is hybrid strain to paddy rice late；Using the three hybridization method for group matching handed over, loose panicle gene is polymerized with scent gene
To in two-line sterile line；Planted with the different light and temperature conditions that can educate the phase by the two-line sterile line infertility phase, and through the fringe in many generations
The identification of type, fragrance, sterility and fertility, Comprehensive Traits screening, be bred as that loose panicle type, rice aroma are strong, sterility is stable,
Coordinate force is good, breeding, the production of hybrid seeds and hybrid yield excellent fragrant rice two-line sterile line high.It is the selection efficiency high, profitable, educate
Into the fringe portion pest and disease damage such as hybrid yield high, rice is of fine quality, nutrition good, the anti-false smut of two-line sterile line combo.
The content of the invention
It is an object of the invention to provide a kind of selection of loose panicle type perfume rice two-line sterile line.
The present invention is with two-line sterile line of rice（One is dual-purpose）, loose panicle type rice strain, fragrant rice strain, eary maturity of rice recovery
System, ripe restorer is hybrid strain to paddy rice late；Using the three hybridization method for group matching handed over, loose panicle gene and scent gene are aggregated to
In two-line sterile line；By two-line sterile line infertility the phase and can educate the phase different light and temperature conditions plant, and through the fringe type in many generations,
The identification of fragrance, sterility and fertility, Comprehensive Traits screening, have been bred as that loose panicle type, rice aroma are strong, sterility is stable, have matched somebody with somebody
With joint efforts well, breeding, the production of hybrid seeds and hybrid yield excellent fragrant rice two-line sterile line high.The selection efficiency high, profitable, incubation
The fringe portion pest and disease damage such as hybrid yield high, rice is of fine quality, nutrition good, the anti-false smut of two-line sterile line combo.
To achieve the above objectives, the present invention uses following technical scheme：
1）Juvenile stage：Two-line sterile line of rice is selected for hybrid strain P1, loose panicle type rice strain is P2, fragrant rice strain is P3,
Eary maturity of rice restorer is P4, and ripe restorer is P5 to paddy rice late；
2）Hybridization combo and screening technique：
a）In the educated phase of two-line sterile line, make hybridization P1/P2, F must be hybridized1Seed, with F1- 12 represent；
b）Plantation F1- 12 plant 40-45 plants, after heading stage eliminates pseudostationary, make three and hand over combination P1/P2//P3, obtain three friendship F1Generation
Seed, F is handed over three1- 123 represent；
c）In the sterile phase of two-line sterile line, F is handed in plantation three1- 123 plant 3500-4000 plants, full heading time is to isolating
Loose panicle infertility individual plant carries out pollen microscopic examination, and selection sterile degree is 100% individual plant；And the Objective that should possess by two-line sterile line
Shape carries out evaluation and screening, and objective trait includes：The 80-125 days time of infertility, 65-85 centimetres of plant height, 20-30 centimetres of spike length, fringe grain
Number 90-140, spike number 12-18 are individual, grain length-width ratio 2.5-4.5,20-30 grams of mass of 1000 kernel, column cap is larger and exposing ratio is higher；
Meanwhile, the blade to being selected in individual plant carries out fragrance identification, listing mark；Maturity period to loose panicle, fragrance, infertility thoroughly, and
Meeting the sterile individual plant of two-line sterile line breeding objective carries out cutting pocket, moves on to about 800 meters of low-temperature regions or artificial of height above sea level
Climate box is planted, and carries out Fertility identification, selects sterile phase pollen sterility thorough and the sterile conversion temperature list close with parent P1
Strain, listing mark, and bagging；Maturity period point individual plant sowing, brown rice fragrance identification is carried out after seed shelling, removes British plain spirits list
Strain, retains the dulcet single-strain seed of tool, obtains three friendship F2For seed, F is handed over three2- 123 represent；
d）In the sterile phase of two-line sterile line, F is handed in strain sowing three2- 123, each strain plants 30-35 plants, full heading time
To unseparated loose panicle infertility strain, pollen microscopic examination is carried out, selection sterile degree is 100% individual plant；And should have by two-line sterile line
Standby objective trait carries out evaluation and screening；Meanwhile, taking 5 blades of individual plant to selected strain carries out fragrance identification, listing mark；
Maturity period, to thoroughly, and meeting the sterile individual plant of two-line sterile line breeding objective and carrying out cutting pocket with loose panicle, fragrance, infertility, moves
To with about 800 meters of low-temperature region or growth cabinet plantations of height above sea level, Fertility identification is carried out, the sterile phase pollen sterility of selection is thorough
Bottom and the sterile conversion temperature individual plant close with parent P1, listing mark, and bagging；Maturity period point individual plant sowing, seed shelling
After carry out brown rice fragrance identification, remove British plain spirits individual plant, retain the dulcet single-strain seed of tool, obtain three friendship F3For seed, with three
Hand over F3- 123 represent；
e）Repeat step d）, in the sterile phase of two-line sterile line, strain sowing F3F is handed over for seed three3- 123, obtain three friendship F4In generation, plants
Son, F is handed over three4- 123 represent；F is handed over by three4- 123 continue to sow and harvest seed, until obtaining three hands over F6- 123 seeds；
f）In the sterile phase of two-line sterile line, F is handed in strain plantation three6- 123, Poa linn P4 and late ripe restorer P5；Utilize
P4 and P5 hands over F to three6- 123 strains carry out test cross, to identify the breeding time feature and potential advantages of sterile strain, obtain test cross F1-
1 and F1-2；And three hand over F7- 123 seeds；
g）In the sterile phase of two-line sterile line, F is handed in strain plantation three7- 123, each strain plants 30-35 plants, test cross F1- 1 and
F1- 2 each 30.To 30 test cross F1- 1 and F1- 2 carry out Yield comparison experiment, filter out respectively with loose panicle, aromatic flavour, comprehensive
Close the excellent F of proterties1- 1 and F1- 2 combinations, its corresponding three friendships F7- 123 sterile strains are selected two-line sterile line, that is, be bred as
Loose panicle type perfume rice two-line sterile line.
Above-mentioned two-line sterile line of rice P1 is SE21s or extensively accounts for 63s.Paddy rice loose panicle type strain P2 isspr8（Chen Pingping,
Ye Shenghai, Lu Yanting, gold are celebrated one's birthday, the feature of the loose panicle mutant spr8 of Zhang little Ming evenings japonica rice Zhejiang round-grained rice 22 and its mutation
Assignment of genes gene mapping nuclear agricultural science reports, 2013,27,1-8.）Orsp（Liu Dan, Wang Jiayu, Liu Jin, Ma Dianrong, Zhao Minghui, it is old
Botany Gazettes, 2015,50 (2) are positioned at the beginning of the genetic analysis of Wenzhou-Fuzhou paddy rice bulk fringe mutant sp and gene: 198–
205）, fragrant rice strain P3 is Lu perfume (or spice) 618；Eary maturity of rice restorer P4 is Minghui 2155, and ripe restorer P5 is that another name for Sichuan Province is extensive to paddy rice late
527。
Beneficial effects of the present invention：
Effect of the invention is that mingling friendship method for group matching using three, it is not that loose panicle gene and scent gene are aggregated into paddy rice two
In educating and being, loose panicle, the fragrant rice two-line sterile line of aromatic flavour are bred as.The selection efficiency high, profitable, the loose panicle perfume of incubation
The fringe portion pest and disease damages such as hybrid yield high, rice is of fine quality, nutrition good, the anti-false smut of rice two-line sterile line combo.Plant the sterile line
The scented rice Hybrid Rice Combination of preparation, can reduce Pesticide use amount, reduce environmental pollution, increase farmers' income.Plant the scented rice rice
Kind, every mu increases yield 5-8% than common rice kind, reduces dispenser cost 70-95 units, and scented rice paddy purchasing price increases
20-25%, every mu can increase economic benefit 330-410 units for peasant, while reducing pollution of the agricultural chemicals to environment.
Specific embodiment
Embodiment 1：
a）In the educated phase of two-line sterile line, two-line sterile line SE21s is selected（P1）, loose panicle type rice strainspr8（P2）As miscellaneous
Parent is handed over, makees hybridization P1/P2, F must be hybridized1Seed, with F1- 12 represent；
b）Plantation F1- 12 40 plants of plant.After heading stage eliminates pseudostationary, the fragrant rice strain Lu perfume (or spice) 618 of choosing（P3）Parent is handed over as three,
Make three and hand over combination P1/P2//P3, obtain three friendship F1For seed, F is handed over three1- 123 represent；
c）In the sterile phase of two-line sterile line, F is handed in plantation three1- 123 3820 plants of plant.Full heading time is to the loose panicle isolated
Sterile individual plant carries out pollen microscopic examination, and selection sterile degree is 100% individual plant；And enter by the objective trait that two-line sterile line should possess
Row evaluation and screening.Meanwhile, carry out fragrance identification, 320 individual plants of listing mark to being selected in 968 blades of individual plant；Maturity period pair
With loose panicle, fragrance, infertility thoroughly, and meet 320 of two-line sterile line breeding objective sterile individual plants and carry out cutting pocket, move on to tool
There are about 800 meters of low-temperature regions of height above sea level to plant, carry out Fertility identification, select sterile phase pollen sterility thorough and sterile conversion temperature
The individual plant close with parent P1 SE21s, 256 individual plants of listing mark, and bagging；Maturity period point individual plant sowing, after seed shelling
Brown rice fragrance identification is carried out, British plain spirits individual plant is removed, retains the dulcet single-strain seed of tool, obtain 206 three friendship F2For individual plant kind
Son, F is handed over three2- 123 represent；
d）In the sterile phase of two-line sterile line, strain sows 206 three friendship F2- 123, each strain plants 35 plants.Heading is bloomed
Phase, to unseparated loose panicle infertility strain, carries out pollen microscopic examination, and selection sterile degree is 100% individual plant；And should by two-line sterile line
The objective trait for possessing carries out evaluation and screening.Meanwhile, taking 5 blades of individual plant to selected strain carries out fragrance identification, and be listed mark
156 individual plants of note；Maturity period with loose panicle, fragrance, infertility to thoroughly, and meeting the sterile individual plant of two-line sterile line breeding objective
Carry out cutting pocket, move on to about 800 meters of low-temperature region plantations of height above sea level, carry out Fertility identification, the sterile phase pollen sterility of selection is thorough
Bottom and the sterile conversion temperature individual plant close with parent P1 SE21s, 106 strains of listing mark, and bagging；Maturity period point is single
Strain sowing, brown rice fragrance identification is carried out after seed shelling, removes British plain spirits individual plant, retains the dulcet single-strain seed of tool, obtains 83
Individual strain three hands over F3For seed, F is handed over three3- 123 represent；
e）In the sterile phase of two-line sterile line, strain sows 83 three friendship F3- 123, each strain plants 35 plants.Full heading time
To unseparated loose panicle infertility strain, pollen microscopic examination is carried out, selection sterile degree is 100% individual plant；And should have by two-line sterile line
Standby objective trait carries out evaluation and screening.Meanwhile, taking 5 blades of individual plant to selected strain carries out fragrance identification, listing mark
65 strains；Maturity period with loose panicle, fragrance, infertility to thoroughly, and meeting the sterile individual plant of two-line sterile line breeding objective and entering
Row cuts pocket, moves on to about 800 meters of low-temperature region plantations of height above sea level, carries out Fertility identification, and the sterile phase pollen sterility of selection is thorough
And the infertility conversion temperature individual plant close with parent P1 SE21s, 45 strains of listing mark, and bagging；Maturity period point individual plant is received
Kind, brown rice fragrance identification is carried out after seed shelling, British plain spirits individual plant is removed, retain the dulcet single-strain seed of tool, obtain 36 strains
It is three friendship F4For seed, F is handed over three4- 123 represent；
f）Continue step e）Until obtaining strain three hands over F6-123.In the sterile phase of two-line sterile line, F is handed in strain plantation three6- 123,
Poa linn（P4）With slow ripe restorer（P5）, 35 plants of each strain plantation.Using P4 and P5 F is handed over to three6- 123 strains 36
It is individual to carry out test cross, to identify the breeding time feature and potential advantages of sterile strain, obtain test cross F1- 1 and F1-2；And three hand over F7-
123 seeds.
g）In the sterile phase of two-line sterile line, F is handed in strain plantation three7- 123, each strain plants 35 plants, test cross F1- 1 and
F1- 2 each 36.To 36 test cross F1- 1 and F1- 2 carry out Yield comparison experiment, filter out respectively with loose panicle, aromatic flavour, comprehensive
Close the excellent F of proterties1- 1 and F1- 2 combinations, its corresponding three friendships F7- 123 sterile strains the 16th are selected two-line sterile line,
It has been bred as loose panicle type perfume rice two-line sterile line " dissipating perfume 1s "；
" dissipating perfume 1s " " dissipates 1 liang of excellent 527 " of perfume, every mu of average product compares reference substance with the scented rice Hybrid Rice Combination of extensive 527 combo in another name for Sichuan Province
Planting II excellent bright 86 increases yield 5%, reduces 70 yuan of dispenser cost, and scented rice paddy purchasing price increases by 20%, and every mu can increase for peasant
Plus 330 yuan of economic benefit.
Embodiment 2：
a）In the educated phase of two-line sterile line, two-line sterile line is selected extensively to account for 63s（P1）, loose panicle type rice strainspr8（P2）Make
It is hybrid strain, makees hybridization P1/P2, F must be hybridized1Seed, with F1- 12 represent；
b）Plantation F1- 12 plant 40-45 plants.After heading stage eliminates pseudostationary, the fragrant rice strain Lu perfume (or spice) 618 of choosing（P3）Parent is handed over as three
This, makees three and hands over combination P1/P2//P3, obtains three friendship F1For seed, F is handed over three1- 123 represent；
c）In the sterile phase of two-line sterile line, F is handed in plantation three1- 123 3760 plants of plant.Full heading time is to the loose panicle isolated
Sterile individual plant carries out pollen microscopic examination, and selection sterile degree is 100% individual plant；And enter by the objective trait that two-line sterile line should possess
Row evaluation and screening.Meanwhile, the blade to being selected in 852 individual plants carries out fragrance identification, 298 individual plants of listing mark；Maturity period is to tool
Having loose panicle, fragrance, infertility thoroughly, and meet 298 of two-line sterile line breeding objective sterile individual plants carries out cutting pocket, and moving on to has
The low-temperature regions plantation of about 800 meters of height above sea level, carries out Fertility identification, the sterile phase pollen sterility of selection is thorough and sterile conversion temperature with
Parent P1 extensively accounts for the close individual plants of 63s, 243 individual plants of listing mark, and bagging；Maturity period point individual plant sowing, after seed shelling
Brown rice fragrance identification is carried out, British plain spirits individual plant is removed, retains the dulcet single-strain seed of tool, obtain 195 three friendship F2For individual plant kind
Son, F is handed over three2- 123 represent；
d）In the sterile phase of two-line sterile line, strain sows 195 three friendship F2- 123, each strain plants 35 plants.Heading is bloomed
Phase, to unseparated loose panicle infertility strain, carries out pollen microscopic examination, and selection sterile degree is 100% individual plant；And should by two-line sterile line
The objective trait for possessing carries out evaluation and screening.Meanwhile, taking 5 blades of individual plant to selected strain carries out fragrance identification, and be listed mark
137 individual plants of note；Maturity period with loose panicle, fragrance, infertility to thoroughly, and meeting the sterile individual plant of two-line sterile line breeding objective
Carry out cutting pocket, move on to about 800 meters of low-temperature region plantations of height above sea level, carry out Fertility identification, the sterile phase pollen sterility of selection is thorough
Bottom and sterile conversion temperature extensively accounts for the close individual plants of 63s, 95 strains of listing mark, and bagging with parent P1；Maturity period point is single
Strain sowing, brown rice fragrance identification is carried out after seed shelling, removes British plain spirits individual plant, retains the dulcet single-strain seed of tool, obtains 69
Individual strain three hands over F3For seed, F is handed over three3- 123 represent；
e）In the sterile phase of two-line sterile line, strain sows 69 three friendship F3- 123, each strain plants 35 plants.Full heading time
To unseparated loose panicle infertility strain, pollen microscopic examination is carried out, selection sterile degree is 100% individual plant；And should have by two-line sterile line
Standby objective trait carries out evaluation and screening.Meanwhile, taking 5 blades of individual plant to selected strain carries out fragrance identification, listing mark
52 strains；Maturity period with loose panicle, fragrance, infertility to thoroughly, and meeting the sterile individual plant of two-line sterile line breeding objective and entering
Row cuts pocket, moves on to about 800 meters of low-temperature region plantations of height above sea level, carries out Fertility identification, and the sterile phase pollen sterility of selection is thorough
And sterile conversion temperature extensively accounts for the close individual plants of 63s, 48 strains of listing mark, and bagging with parent P1；Maturity period point individual plant
Sowing, brown rice fragrance identification is carried out after seed shelling, removes British plain spirits individual plant, retains the dulcet single-strain seed of tool, obtains 35
Strain three hands over F4For seed, F is handed over three4- 123 represent；
f）Continue step e）Until obtaining strain three hands over F6-123.In the sterile phase of two-line sterile line, F is handed in strain plantation three6- 123,
Poa linn（P4）With slow ripe restorer（P5）, 35 plants of each strain plantation.Using P4 and P5 F is handed over to three6- 123 strains 35
It is individual to carry out test cross, to identify the breeding time feature and potential advantages of sterile strain, obtain test cross F1- 1 and F1-2；And three hand over F7-
123 seeds.
g）In the sterile phase of two-line sterile line, F is handed in strain plantation three7- 123, each strain plants 35 plants, test cross F1- 1 and
F1- 2 each 35.To 35 test cross F1- 1 and F1- 2 carry out Yield comparison experiment, filter out respectively with loose panicle, aromatic flavour, comprehensive
Close the excellent F of proterties1- 1 and F1- 2 combinations, its corresponding three friendships F7- 123 sterile strains the 29th are selected two-line sterile line,
It has been bred as loose panicle type perfume rice two-line sterile line " dissipating perfume 2s "；
" dissipating perfume 2s " " dissipates 2 liang of excellent 527 " of perfume, every mu of average product compares reference substance with the scented rice Hybrid Rice Combination of extensive 527 combo in another name for Sichuan Province
Planting II excellent bright 86 increases yield 6%, reduces 95 yuan of dispenser cost, and scented rice paddy purchasing price increases by 25%, and every mu can increase for peasant
Plus 410 yuan of economic benefit.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to covering scope of the invention.
Claims (2)
1. the selection of a kind of loose panicle type perfume rice two-line sterile line, it is characterised in that the selection include following methods with
Step：
1）Juvenile stage：Two-line sterile line of rice is selected for hybrid strain P1, loose panicle type rice strain is P2, fragrant rice strain is P3,
Eary maturity of rice restorer is P4, and ripe restorer is P5 to paddy rice late；
2）Hybridization combo and screening technique：
a）In the educated phase of two-line sterile line, make hybridization P1/P2, F must be hybridized1Seed, with F1- 12 represent；
b）Plantation F1- 12 plant 40-45 plants, after heading stage eliminates pseudostationary, make three and hand over combination P1/P2//P3, obtain three friendship F1In generation, plants
Son, F is handed over three1- 123 represent；
c）In the sterile phase of two-line sterile line, F is handed in plantation three1- 123 plant 3500-4000 plants；Full heading time is to isolating
Loose panicle infertility individual plant carries out pollen microscopic examination, selects the individual plant that sterile degree is 100%, and the Objective that should possess by two-line sterile line
Shape carries out evaluation and screening, and its objective trait includes：The 80-125 days time of infertility, 65-85 centimetres of plant height, 20-30 centimetres of spike length, fringe
Grain number 90-140, spike number 12-18, grain length-width ratio 2.5-4.5,20-30 grams of mass of 1000 kernel, column cap is larger and exposing ratio compared with
It is high；Meanwhile, the blade to being selected in individual plant carries out fragrance identification, listing mark；Maturity period to loose panicle, fragrance, infertility thoroughly,
And meet the sterile individual plant of two-line sterile line breeding objective and carry out cutting pocket, move on to the low-temperature region with about 800 meters of height above sea level or people
Work climate box is planted, and carries out Fertility identification, selects sterile phase pollen sterility thorough and sterile conversion temperature is close with parent P1
Individual plant, listing mark, and bagging；Maturity period point individual plant sowing, brown rice fragrance identification is carried out after seed shelling, removes British plain spirits list
Strain, retains the dulcet single-strain seed of tool, obtains three friendship F2For seed, F is handed over three2- 123 represent；
d）In the sterile phase of two-line sterile line, F is handed in strain sowing three2- 123, each strain plants 30-35 plants；Full heading time pair
Unseparated loose panicle infertility strain, carries out pollen microscopic examination, and selection sterile degree is 100% individual plant；And should possess by two-line sterile line
Objective trait carry out evaluation and screening；Meanwhile, taking 5 blades of individual plant to selected strain carries out fragrance identification, listing mark；Into
The ripe phase, to thoroughly, and meeting the sterile individual plant of two-line sterile line breeding objective and carrying out cutting pocket with loose panicle, fragrance, infertility, moves on to
With about 800 meters of low-temperature region or growth cabinet plantations of height above sea level, Fertility identification is carried out, the sterile phase pollen sterility of selection is thorough
And the infertility conversion temperature individual plant close with parent P1, listing mark, and bagging；Maturity period point individual plant sowing, after seed shelling
Brown rice fragrance identification is carried out, British plain spirits individual plant is removed, retains the dulcet single-strain seed of tool, obtain three friendship F3For seed, handed over three
F3- 123 represent；
e）Repeat step d）, in the sterile phase of two-line sterile line, F is handed in strain sowing three3- 123, obtain three friendship F4For seed, handed over three
F4- 123 represent；F is handed over by three4- 123 seeds continue to sow and harvest seed, until obtaining three hands over F6- 123 seeds；
f）In the sterile phase of two-line sterile line, F is handed in strain plantation three6- 123, Poa linn P4 and late ripe restorer P5；Utilize
P4 and P5 hands over F to three6- 123 strains carry out test cross, to identify the breeding time feature and potential advantages of sterile strain, obtain test cross F1-
1 and F1-2；And F7- 123 seeds；
g）In the sterile phase of two-line sterile line, F is handed in strain plantation three7- 123, test cross F1- 1 and F1- 2 each 30；To 30 test crosses
F1- 1 and F1- 2 carry out Yield comparison experiment, filter out respectively with the excellent F of loose panicle, aromatic flavour, Comprehensive Traits1- 1 and F1-
2 combinations, its corresponding three friendships F7- 123 sterile strains are selected two-line sterile line, that is, it is infertility to be bred as loose panicle type perfume rice two
System.
2. a kind of selection of loose panicle type perfume rice two-line sterile line according to claim 1, it is characterised in that the water
Rice two-line sterile line P1 is SE21s or extensively accounts for 63s, and loose panicle type rice strain P2 isspr8Orsp, fragrant rice strain P3 is fragrant Lu
618, eary maturity of rice restorer P4 are Minghui 2155, and ripe restorer P5 is another name for Sichuan Province extensive 527 to paddy rice late.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710047683.XA CN106888959B (en) | 2017-01-22 | 2017-01-22 | A kind of selection of loose panicle type perfume (or spice) rice two-line sterile line |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710047683.XA CN106888959B (en) | 2017-01-22 | 2017-01-22 | A kind of selection of loose panicle type perfume (or spice) rice two-line sterile line |
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| CN106888959A true CN106888959A (en) | 2017-06-27 |
| CN106888959B CN106888959B (en) | 2018-10-23 |
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Cited By (4)
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| CN109006142A (en) * | 2018-08-17 | 2018-12-18 | 全州县咸水虹桥米业有限公司 | A kind of method of walnut and upland rice interplanting |
| CN111280047A (en) * | 2020-02-12 | 2020-06-16 | 安徽袁粮水稻产业有限公司 | Breeding method of rice two-line sterile line with excellent performance |
| CN111280048A (en) * | 2020-02-12 | 2020-06-16 | 安徽袁粮水稻产业有限公司 | Breeding method of two-line sterile line of photic leaf type rice |
| CN111727877A (en) * | 2020-07-29 | 2020-10-02 | 重庆市农业科学院 | Method for cultivating high-quality dual-purpose genic male sterile line of fragrant rice |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006142A (en) * | 2018-08-17 | 2018-12-18 | 全州县咸水虹桥米业有限公司 | A kind of method of walnut and upland rice interplanting |
| CN111280047A (en) * | 2020-02-12 | 2020-06-16 | 安徽袁粮水稻产业有限公司 | Breeding method of rice two-line sterile line with excellent performance |
| CN111280048A (en) * | 2020-02-12 | 2020-06-16 | 安徽袁粮水稻产业有限公司 | Breeding method of two-line sterile line of photic leaf type rice |
| CN111727877A (en) * | 2020-07-29 | 2020-10-02 | 重庆市农业科学院 | Method for cultivating high-quality dual-purpose genic male sterile line of fragrant rice |
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| CN106888959B (en) | 2018-10-23 |
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