CN106886001A - A kind of controllable magnetic field generating means for biological study - Google Patents
A kind of controllable magnetic field generating means for biological study Download PDFInfo
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- CN106886001A CN106886001A CN201710293300.7A CN201710293300A CN106886001A CN 106886001 A CN106886001 A CN 106886001A CN 201710293300 A CN201710293300 A CN 201710293300A CN 106886001 A CN106886001 A CN 106886001A
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- G—PHYSICS
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- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/28—Details of apparatus provided for in groups G01R33/44 - G01R33/64
- G01R33/38—Systems for generation, homogenisation or stabilisation of the main or gradient magnetic field
- G01R33/389—Field stabilisation, e.g. by field measurements and control means or indirectly by current stabilisation
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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Abstract
The invention discloses a kind of controllable magnetic field generating means for biological study, including：Magnetic source for producing required magnetic field, magnetic source includes several coil groups；For the control device of the energization situation of control coil group, control device signal is connected to magnetic source.Precise control is carried out to the energization situation of the coil group in magnetic source by control device, electric magnetisation, such that it is able to accurately and efficiently control magnetic field production, magnetic field needed for Bioexperiment can be provided, operation object can specifically include the cell that the gene that will express target protein by gene Transfection Technology is imported jointly with magnetic medium, tissue, organ, whole animal, overall plant, or import the cell of magnetic strength albumen, tissue, organ, whole animal, overall plant, range of application is wider, and convenient experimental operation, be conducive to improving the accuracy of conventional efficient and experimental result.
Description
Technical field
The present invention relates to field of bioexperiment technology, more particularly to a kind of controllable magnetic field for biological study is filled
Put.
Background technology
The changes of the operation object under magnetic fields such as research cell, tissue, organ, whole animal, overall plant are raw
One of important research direction of thing engineering, its result of study is significant.
However, at present when magnetic control is carried out to operation object, it is impossible to precise control is carried out to required magnetic field, so that cannot
It is accurate to obtain required magnetic field, influence the accuracy of experimental result.
Therefore, how the generation in precise control magnetic field, be the current technical issues that need to address of those skilled in the art.
The content of the invention
In view of this, it is an object of the invention to provide a kind of controllable magnetic field generating means for biological study, can
The generation in precise control magnetic field.
To achieve the above object, the present invention provides following technical scheme：
A kind of controllable magnetic field generating means for biological study, including：
Magnetic source for producing required magnetic field, the magnetic source includes several coil groups；
Control device for controlling the energization situation of the coil group, the control device signal is connected to the magnetic
Source.
Preferably, the coil group is three, and three coil groups are corresponded to around X-axis, Y-axis, Z axis winding respectively, and three
The individual coil group in three-dimensional sphere alternate, wherein, X-axis, Y-axis, Z axis are vertical two-by-two.
Preferably, the control device includes the electric current for corresponding to each coil group generation magnetic field of control respectively
Regulation device.
Preferably, the current regulation device includes alternating magnetic field control module, pulsed magnetic field control module, pulsation magnetic
Field control module and constant voltage DC control module.
Preferably, the constant voltage DC control module includes at least two optional magnitudes of voltage.
Preferably, each described current regulation device connects a switch controlling device, all switch controls respectively
Device is connected to same power input.
Preferably, the coil is mounted on whirligig, and the control device signal is connected to the whirligig, institute
Stating control device can control the rotating speed of the whirligig.
Preferably, the whirligig includes at least two liftable supports, is arranged at the bottom of the liftable support
Swivel base and the liftable acceptor bearing device on the swivel base, the coil is mounted on the liftable
The top of support, the liftable support, the acceptor bearing device, the swivel base difference signal connection control dress
Put.
Preferably, the outside of the coil group is sequentially provided with least two scalable components, described scalable group around circumferential
Part can extrude the coil group towards the center of the coil group.
Preferably, the scalable component is including for the first dividing plate being fixedly connected with the coil group and by spring
The second partition being connected with first dividing plate, the first dividing plate signal is connected to the control device, the control device
First dividing plate can be controlled to move.
Controllable magnetic field generating means provided by the present invention for biological study includes magnetic source and control device.By control
Device processed carries out precise control, electric magnetisation, such that it is able to accurately and efficiently control magnetic to the energization situation of the coil group in magnetic source
Field production, using the teaching of the invention it is possible to provide the magnetic field needed for Bioexperiment, operation object can specifically include will by gene Transfection Technology
Cell, tissue, organ, whole animal, overall plant, importing magnetic that the gene of expression target protein is imported jointly with magnetic medium
Sense albumen and other cell, tissue, organ, whole animal, entirety plant, range of application is wider, and experimental implementation side
Just, be conducive to improving the accuracy of conventional efficient and experimental result.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Inventive embodiment, for those of ordinary skill in the art, on the premise of not paying creative work, can also basis
The accompanying drawing of offer obtains other accompanying drawings.
Fig. 1 is the structural representation of the specific embodiment one of controllable magnetic field generating means provided by the present invention；
Fig. 2 is the structural representation of the coil group of specific embodiment one of controllable magnetic field generating means provided by the present invention；
Fig. 3 is the structural representation of the specific embodiment two of controllable magnetic field generating means provided by the present invention；
Fig. 4 is the structural representation of the specific embodiment three of controllable magnetic field generating means provided by the present invention；
Fig. 5 be controllable magnetic field generating means provided by the present invention specific embodiment three in compressible component structural representation
Figure；
Fig. 6 is TGF protein expression glue figure.
In Fig. 1 to Fig. 5,1 is coil group, and 2 is current regulation device, and 3 is switch controlling device, and 4 is power input,
5 is liftable support, and 6 is acceptor bearing device, and 7 is swivel base, and 8 is the first dividing plate, and 9 is spring, and 10 is second partition.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Core of the invention is to provide a kind of controllable magnetic field generating means for biological study, being capable of precise control magnetic
The generation of field.
Refer to the structure of Fig. 1 and the specific embodiment one that Fig. 2, Fig. 1 are controllable magnetic field generating means provided by the present invention
Schematic diagram；Fig. 2 is the structural representation of the coil group of specific embodiment one of controllable magnetic field generating means provided by the present invention.
In a kind of specific embodiment for the controllable magnetic field generating means of biological study provided by the present invention, including
Magnetic source for producing required magnetic field, magnetic source includes several coil groups 1；Also include the energization feelings for control coil group 1
The control device of condition, control device signal connection magnetic source.
Precise control, electric magnetisation, such that it is able to essence are carried out to the energization situation of the coil group 1 in magnetic source by control device
Really efficiently control magnetic field production, using the teaching of the invention it is possible to provide the magnetic field needed for Bioexperiment, operation object can specifically include passing through
Gene Transfection Technology will express the cell that gene and the magnetic medium of target protein import jointly, tissue, organ, whole animal,
Overall plant, or cell, tissue, organ, whole animal, the overall plant of magnetic strength albumen are imported, range of application is wider, and experiment
It is easy to operate, be conducive to improving the accuracy of conventional efficient and experimental result.
In specific embodiment provided by the present invention, coil group 1 is specifically as follows three, and three coil groups 1 are corresponded to respectively
Around X-axis, Y-axis, Z axis winding, and three coil groups 1 are in three-dimensional sphere alternate, wherein, X-axis, Y-axis, Z axis are vertical two-by-two.
Wherein, operation object can be placed among the inner space that three coil groups 1 are formed.
The setting of three coil groups 1 of dimension can ensure that operation object can be subject to the magnetic in magnetic field in all directions
Power, magneticaction scope is wider.
Further, can include for corresponding to the electric current for controlling each coil group 1 to produce magnetic field respectively in control device
Regulation device 2.That is, the intensity of the electric current of each coil group 1 generation, direction and frequency can be controlled independently, so that
Different magnetic fields are produced, field controllable scope is improved.
Further, alternating magnetic field control module, pulsed magnetic field control mould can be specifically included in current regulation device 2
Block, pulsating field control module, constant voltage DC control module, conveniently and efficiently to produce different electric currents, so as to produce difference
The magnetic field of form, further expands field controllable scope.
Wherein, constant voltage DC control module can specifically include at least two optional magnitudes of voltage, such that it is able to straight in voltage stabilizing
Under stream mode, by the magnetic field intensity for selecting different magnitudes of voltage to realize different.
In above-mentioned each embodiment, each current regulation device 2 can respectively connect a switch controlling device 3, own
Switch controlling device 3 can be connected to same power input 4.That is, the current switching of each coil group 1 can be received respectively
To control such that it is able to which the current switching according to coil group 1 produces one-dimensional magnetic field, two-dimensional magnetic field, three-dimensional magnetic field, further improves
The controllability in magnetic field.
Fig. 3 is refer to, Fig. 3 is the structural representation of the specific embodiment two of controllable magnetic field generating means provided by the present invention
Figure.
In specific embodiment provided by the present invention two, coil group 1 can specifically be set on the spinning device, control device letter
Number the whirligig is connected to, control device can control the rotating speed of the whirligig.Entered by whirligig band moving winding group 1
Row rotation, can produce moving field in rotary course, easy to operate.
Further, whirligig can include at least two liftable supports 5, be arranged at the bottom of liftable support 5
Swivel base 7 and the liftable acceptor bearing device 6 on swivel base 7, coil group 1 is located at liftable support 5
Top, liftable support 5, acceptor bearing device 6, the difference signal connection control device of swivel base 7.
The lifting of liftable support 5 is controlled by control device, the angle in magnetic field can be produced with regulating winding group 1, passed through
The height of control device control acceptor bearing device 6, can be with the distance between regulating winding group 1 and operation object, to adjust magnetic
Field intensity, the rotation in magnetic field is controlled by control device, can generate required moving field.
Wherein, the parts such as motor, screw nut assembly can be specifically set in control device to realize elevating control.
It can be seen that, by liftable support 5, acceptor bearing device 6 and rotating base, magnetic can be controlled by various methods
Direction, the intensity of field, control more flexible.
Further, the bottom of rotating base can set runner, whirligig is moved freely on platform, from
And further facilitate operation.
Refer to the structure of Fig. 4 and the specific embodiment three that Fig. 5, Fig. 4 are controllable magnetic field generating means provided by the present invention
Schematic diagram；Fig. 5 be controllable magnetic field generating means provided by the present invention specific embodiment three in compressible component structural representation
Figure.
In specific embodiment provided by the present invention three, the outside of coil group 1 can be sequentially provided with least two around circumference can
Telescopic component, scalable component can extrude coil group 1 towards the center of coil group 1, by extruding coil group 1, make coil group 1
Deform upon, the intensity in magnetic field can be conveniently adjusted and position is produced.
Further, scalable component can be including for the first dividing plate 8 being fixedly connected with coil group 1 and by spring
9 second partitions 10 being connected with the first dividing plate 8, the signal of the first dividing plate 8 is connected to control device, and control device can control first
Dividing plate 8 is moved.Wherein, second partition 10 can be fixedly installed on wall, other machines housing or at other positions.It is this kind of
In embodiment, coil group 1 is extruded by the first dividing plate 8, the magnetic field to form point, line, surface can be combined, the setting of other springs 9
Row buffering can be entered in motion to the first dividing plate 8, be conducive to improving the accuracy for controlling.
Specifically, scalable component can be with circumferentially disposed four of coiling group 1, to realize each side to coil group 1
Xiang Jieke is extruded, and is conducive to improving the flexibility for controlling.
In above-mentioned each embodiment, the coil in coil group 1 is specifically as follows the coils such as solenoid, Helmholtz coil,
In addition, coil inside can be optionally provided with permanent magnet, ferromagnet etc..
A kind of application of the above-mentioned controllable magnetic field generating means in specific experiment is as follows：
Main agents and instrument：Mouse lymphocyte, purchased from American. tissue culture collection (ATCC)；CCK-8 reagents
Box, purchased from Guangzhou Jing Xin bio tech ltd；Hyclone (FBS), is purchased from Invitrrogen companies of the U.S.；Sword bean egg
(ConA) and lipopolysaccharides (LPS) in vain, purchased from Sigma Co., USA；CO2Incubator, purchased from German Binder companies；
Multiskan Ascent multi-function microplate readers, purchased from Thermo companies of the U.S.；Self-control possesses and freely adjust two and three dimensions mould
The controllable magnetic field generating means of formula.
TGF channel genes lymphocyte：Gene amplification primer is designed according to TGF gene order
Thing, is imported using plasmid, obtains plasmid expression vector, and transfect to lymphocyte.TGF protein content about 25kD, with
The expression of Western blotting (Western blot, WB) technical Analysis TGF gene.
Influence of the magnetic field to mouse lymphocyte proliferative conditions：Mouse lymphocyte proliferative conditions are entered using CCK-8 methods
Row is determined.Take the logarithm the lymphocyte in growth period, it is suitable concentration (5 × 106/ to adjust cell number with the culture medium containing 10%FBS
Ml), 96 porocyte culture plates are added, per the μ l of hole 90, each sets 10 multiple holes, in 37 DEG C, 5%CO2Under the conditions of cultivate 24h；Point
If five experimental groups, experimental group A groups are self-control magnetic of the lymphocyte culture in two-dimensional model for importing TGF gene
In field device, experimental group B groups for import TGF gene lymphocyte culture in the self-control magnetic field of three dimensional pattern,
Control group C groups are that the lymphocyte without TGF gene is placed in the self-control magnetic field of two-dimensional model, untreated fish group D
Lymphocyte of the group without TGF gene is placed directly in incubator, and E groups only have culture medium.Tri- groups of A, B, C's
Magnetic field intensity is 0.4T, and five groups of cells are all 37 DEG C, 5%CO2Under the conditions of cultivate 10 respectively, 20,30,40, carry out after 50h
CCK-8 detects that the CCK-8 of final concentration of 10 μ l is added per hole, and 4h is cultivated in continuation.Under 450nm wavelength, ELIASA detection absorbance
(OD) value.The proliferation rate of lymphocyte under the conditions of calculating respectively.Experiment is independently repeated 3 times.
Proliferation rate=(ODA/B-ODD)/(ODC-ODD)
TGF expression of gene protein result：Gene containing TGF is prepared by Western methods
Lymphocyte (A groups) and the lymphocyte (B groups) without TGF gene gel electrophoresis glue figure, contrast A, B,
Tri- groups of results of C, the gene expression of display A group TGFs preferably, take second place by B groups, and it is most weak that C groups are expressed.Refer to Fig. 6.
The influence of different magnetic field and TGF gene pairs lymphocyte growth situation：In each condition pair of different time
As shown in table 1, the cell proliferation rate that preceding 10 hour each groups are cultivated in the early stage is maximum for the influence of mouse lymphocyte propagation.A groups
It is 3.40 in the 10th hourly growth rate.In the stage below, proliferation rate change is little, maintains 2.80 or so.B groups are small the 10th
Hourly growth rate is 3.41, and follow-up phase maintains 2.85 or so.C groups are 3.32 in the 10th hourly growth rate, and final stage is maintained
2.70 or so.
B groups cell proliferation rate after 10 hours is slightly above A groups, shows in the case where three-dimensional magnetic field acts on, and compares two-dimensional magnetic field
Promoting cell cultivation effect will get well.The proliferation rate of A groups is consistently higher than C groups, shows to be promoted carefully in TGF gene
Born of the same parents breed.By contrasting the A values of two groups of cells of C, D, the upgrowth situation of the upgrowth situation less than C groups of D group cells is found.Thus see
See magnetic strength gene --- TGF gene under the influence of a magnetic field, promotes cell cultivation effect obvious.
Table 1：The influence (n=30) that each condition of different time is bred to mouse lymphocyte
Each embodiment is described by the way of progressive in this specification, and what each embodiment was stressed is and other
The difference of embodiment, between each embodiment identical similar portion mutually referring to.
The controllable magnetic field generating means for biological study provided by the present invention is described in detail above.This
Apply specific case in text to be set forth principle of the invention and implementation method, the explanation of above example is only intended to
Help understands the method for the present invention and its core concept.It should be pointed out that for those skilled in the art,
Without departing from the principles of the invention, some improvement and modification can also be carried out to the present invention, these are improved and modification also falls
Enter in the protection domain of the claims in the present invention.
Claims (10)
1. a kind of controllable magnetic field generating means for biological study, it is characterised in that including：
Magnetic source for producing required magnetic field, the magnetic source includes several coil groups；
Control device for controlling the energization situation of the coil group, the control device signal is connected to the magnetic source.
2. the controllable magnetic field generating means for biological study according to claim 1, it is characterised in that the coil
Group is three, and three coil groups are corresponded to around X-axis, Y-axis, Z axis winding respectively, and three coil groups are on three-dimensional sphere
It is staggered, wherein, X-axis, Y-axis, Z axis are vertical two-by-two.
3. the controllable magnetic field generating means for biological study according to claim 2, it is characterised in that the control
Device includes the current regulation device for corresponding to each coil group generation magnetic field of control respectively.
4. the controllable magnetic field generating means for biological study according to claim 3, it is characterised in that the electric current
Regulation device includes alternating magnetic field control module, pulsed magnetic field control module, pulsating field control module and constant voltage DC
Control module.
5. the controllable magnetic field generating means for biological study according to claim 4, it is characterised in that the voltage stabilizing
DC control module includes at least two optional magnitudes of voltage.
6. the controllable magnetic field generating means for biological study according to claim 3 to 5 any one, its feature exists
In each described current regulation device connects a switch controlling device respectively, and all switch controlling devices are connected to together
One power input.
7. the controllable magnetic field generating means for biological study according to claim 1, it is characterised in that the coil
It is mounted on whirligig, the control device signal is connected to the whirligig, the control device can control described
The rotating speed of whirligig.
8. the controllable magnetic field generating means for biological study according to claim 7, it is characterised in that the rotation
Device is including at least two liftable supports, the swivel base of the bottom for being arranged at the liftable support and located at the rotation
Liftable acceptor bearing device on chassis, the coil is mounted on the top of the liftable support, the liftable branch
Frame, the acceptor bearing device, swivel base difference signal connect the control device.
9. the controllable magnetic field generating means for biological study according to claim 1, it is characterised in that the coil
The outside of group is sequentially provided with least two scalable components around circumferential, and the scalable component can be towards in the coil group
The heart extrudes the coil group.
10. the controllable magnetic field generating means for biological study according to claim 9, it is characterised in that it is described can
Telescopic component is included for the first dividing plate for being fixedly connected with the coil group and is connected with first dividing plate by spring
Second partition, the first dividing plate signal is connected to the control device, and the control device can control first dividing plate
It is mobile.
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| CN109055187A (en) * | 2018-09-13 | 2018-12-21 | 吉林建筑大学 | A kind of apparatus and method that research magnetic action influences aerobic microbiological |
| CN110326458A (en) * | 2019-07-29 | 2019-10-15 | 贝儿科技(广东)有限公司 | A kind of method and device of naturalized plant multiplication releasing negative oxygen ion |
| CN110837068A (en) * | 2019-11-25 | 2020-02-25 | 中北大学 | Device for maintaining constant uniform magnetic field locally and precisely adjusting direction strength |
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| CN110837068B (en) * | 2019-11-25 | 2021-08-20 | 中北大学 | Device for maintaining constant uniform magnetic field locally and precisely adjusting direction strength |
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| Publication number | Publication date |
|---|---|
| CN106886001B (en) | 2019-10-29 |
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