CN106885764A - A kind of biomembrane anti-permeability detection means and application - Google Patents
A kind of biomembrane anti-permeability detection means and application Download PDFInfo
- Publication number
- CN106885764A CN106885764A CN201510929702.2A CN201510929702A CN106885764A CN 106885764 A CN106885764 A CN 106885764A CN 201510929702 A CN201510929702 A CN 201510929702A CN 106885764 A CN106885764 A CN 106885764A
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- kerve
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- 230000003487 anti-permeability effect Effects 0.000 title claims abstract description 31
- 238000001514 detection method Methods 0.000 title claims abstract description 26
- 239000000463 material Substances 0.000 claims abstract description 21
- 230000002706 hydrostatic effect Effects 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 22
- 238000002474 experimental method Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000004888 barrier function Effects 0.000 claims 1
- RKTYLMNFRDHKIL-UHFFFAOYSA-N copper;5,10,15,20-tetraphenylporphyrin-22,24-diide Chemical compound [Cu+2].C1=CC(C(=C2C=CC([N-]2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3[N-]2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 RKTYLMNFRDHKIL-UHFFFAOYSA-N 0.000 claims 1
- 238000007789 sealing Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 abstract 1
- 210000004379 membrane Anatomy 0.000 description 13
- 239000012528 membrane Substances 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004556 brain Anatomy 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000012620 biological material Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000003670 easy-to-clean Effects 0.000 description 2
- 239000002657 fibrous material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 206010008164 Cerebrospinal fluid leakage Diseases 0.000 description 1
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 241001269524 Dura Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 210000001951 dura mater Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
- G01N15/082—Investigating permeability by forcing a fluid through a sample
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- External Artificial Organs (AREA)
Abstract
The invention provides a kind of reasonable in design, simple structure, easy to operate low pressure biomembrane anti-permeability detection means, the anti-permeability of it is applied to detection density is low, resistance to hydrostatic pressure value is low material.With measurement sensitivity is high, the fixed material of not damaged sealing, there is provided the pressure value of relative broad range.There is the characteristics of can regulating and controlling according to dissimilar material properties to the speed of pressure applied simultaneously.
Description
Technical field
The present invention relates to technical fields such as mechanics of biomaterials, hydrodynamics conduction pressure, more particularly to low-density, resistance to hydrostatic pressure
The detection of the low biomaterial anti-permeability of value.
Background technology
Hard brain (ridge) membrane tissue, is the natural cover for defense for protecting brain and myeloid tissue.Dura defect easily causes initiation cerebrospinal fluid to ooze
Leakage, accordingly, it would be desirable to there are the excellent alternative materials of anti-permeability to repair the dura mater of defect, to meet clinical needs.
In recent years, with the continuous research and development of organizational project and biomaterial subject, the research of biomembrane material is achieved
Considerable degree of progress, researchers expect that it can replace hard brain (ridge) film, protect brain (spinal cord) tissue, reduce postoperative cerebral
The incidence of cerebrospinal fluid leakage.Therefore, an excellent important indicator for being to evaluate biomembrane material performance of anti-permeability.
Type i collagen film is to use the collagen extracted from animal tissue with low concentration, freeze-dried technique and crosslinking process
And the class spongy material with three dimensional pore structures for obtaining.Its collagenous fibres formed exempt from suture film material mechanical property it is low,
Tensile strength (tensile strength) only has 60-150KPa, and the stretching of material, sewing properties are very weak.But due to collagen
Itself has good anthemorrhagic performance, is this material while having good anti-permeability.Principle is connecing for collagenous fibres and blood
Touch, blood platelet and collagenous fibres are quickly combined, be filled in the three-dimensional pore space of material and under the assistance of factor, form viscous
Consistence firm bonding higher.Even so, when material anti-permeability is detected, three-dimensional collagenous fibres are to being added in thereon
Power it is still very sensitive --- excessive, material easy fracture;Too small, inspection does not measure anti-leakage effect of material bonding, is that this is right
Detection low-density, the loose collagenous fiber material of low hydrostatic pressure resistant need a kind of special detection means to complete the survey of mechanical property
Examination.
The clamper of the fixed test sample that existing anti-permeability detection means (the domestic type of YG (B) 812) uses is generally gold
Category material.The test of its anti-permeability for being applied to the fabric with VHD such as high molecular synthetic material, fixed biological
Easily occur damaging sample or poorly sealed phenomenon during membrane sample.In addition, existing anti-permeability detection means applies stressed instantaneous
Speed, measurement sensitivity is relatively low, when the resistance to hydrostatic pressure value of biomembrane is very low, it is difficult to its anti-permeability level is made comment
Valency.
Biomembrane anti-permeability detection means operation principle:Using the principle of hydrodynamics liquid conductive pressure, in device one end
Not damaged seals fixed biofilm, and the device other end contains the liquid of certain volume according to material property, and controls to apply by piston
The speed of liquid feeding pressure.Non-leakage time is kept by can bear maximum hydrostatic of test biology film and at this pressure to judge
Its anti-permeability.
The content of the invention
The invention provides a kind of simple structure, easy to operate, impermeable suitable for the low biomembrane of low-density, resistance to hydrostatic pressure value
Property detection means.Its energy not damaged sealing fixed biofilm material, can provide the pressure value of relative broad range, and apply stressed speed
Degree can be regulated and controled according to dissimilar material properties.
The present invention is achieved by the following technical solutions:
A kind of structure of biomembrane anti-permeability detection means includes kerve, U-tube, fixture and the part of straight tube four.
Preferably, configuration one a length of 20~60cm, a width of 20~40cm, the kerve of a height of 5~10cm, and kerve away from
" convex " type opening is provided with away from 5~20cm of middle position, its size is 1~1.5 times of U-tube, and its two ends is provided with solid
Fixed column, pillar height is 5~50cm, is easy to fix U-tube, and make its holding level;
Preferably, kerve is formed around baffle plate, height of baffle plate is 2~10cm, to prevent containing liquid or experiment completion discharge
Table top is trickled down in straight tube and U-tube during liquid.
Preferably, kerve two ends are provided with 2 delivery ports, respectively apart from base 0.5~1cm and 1~3cm;Latter of which passes through
The rubber hose of a diameter of 0.6~1cm is connected with the discharge outlet in U-tube, and its level height is low compared with U-tube discharge outlet
0.5~2cm, is easy in groove and liquid is discharged in U-tube, it is easy to clean.
The frosted platform energy not damaged sealing fixed biofilm of U-tube and straight tube, and berm width is 0.5~5cm.
U-tube frosted platform and plunger distance 2~10cm of kerve, convenient operation.
The piston control flow rate of liquid of U-tube, realizes that hydrodynamics liquid conductive pressure and control apply the speed of hydraulic pressure.
The internal diameter and height of U-tube can be designed adjustment according to material property, there is provided the pressure value of relative broad range, be applicable it
In all size model biomembrane material different with resistance to hydrostatic ability.
Brief description of the drawings:
Fig. 1 is structural representation of the invention;
Fig. 2 is the top view of Fig. 1;
Specific embodiment
It is biomembrane anti-permeability detection means such as Fig. 1, including kerve 1, U-tube 2, fixture 3 and the part of straight tube 4 four.
" convex " the type opening of kerve 1 matches with U-tube 2, and its two ends is provided with fixed column 1-2, is easy to fix U-tube 2,
And make its holding level;The baffle plate 1-1 of the surrounding of kerve 1, to prevent containing liquid or experiment completion discharge straight tube 3 and U-tube 2
Table top is trickled down during middle liquid.Frosted platform 2-1 and 4-1 the not damaged fixed biofilm of U-tube 2 and straight tube 4, U-tube 2
Piston 2-2 control flow rate of liquid, realize hydrodynamics liquid conductive pressure.The horizontal bottom of the tube of U-tube 2 is provided with discharge outlet
2-2, is directly connected by flexible pipe with one of discharge outlet 1-3 of kerve, is convenient to clean the liquid in U-tube.On kerve
Another discharge outlet 1-2, directly can be connected with waste liquid barrel, be easy to clean the liquid in kerve.
Embodiment 1:The anti-permeability for individually exempting to suture collagem membrane is detected
Apparatus of the present invention are used to detect that specification exempts from suture collagem membrane anti-permeability for 5 × 60 × 60mm.
Take one piece of 5 × 60 × 60mm exempt from suture collagem membrane, be placed on the frosted platform of U-tube, straight tube is placed on collagem membrane,
U-tube and straight tube are completely superposed, are fixed with fixture not damaged.Liquid is contained in U-tube, makes its hydrostatic up to 3cm water
Post (0.294KPa).When experiment starts, piston is opened, it is non-leakage in 3s at this pressure that suture collagem membrane is exempted from observation.
Embodiment 2:Exempt to suture the anti-permeability detection after collagem membrane infiltration blood
Apparatus of the present invention are used to detect that specification exempts from suture collagem membrane anti-permeability for 5 × 60 × 60mm.
Take one piece of 5 × 60 × 60mm exempt from suture collagem membrane, infiltrated 15 seconds in the blood of fresh (without anti-coagulants).To infiltrate new
The collagem membrane of blood liquid is placed on the frosted platform of U-tube, and straight tube is placed on collagem membrane, U-tube and straight tube is weighed completely
Close, fixed with fixture not damaged.Liquid is contained in U-tube, its hydrostatic is reached 30cm water columns (2.94KPa).It is real
When testing beginning, piston is opened, it is non-leakage in 5min at this pressure that suture collagem membrane is exempted from observation.
Conclusion:
This equipment can detect low-density, the loose collagenous fiber material of low hydrostatic pressure resistant and can control hydrostatic 0~5.88Kpa scopes.
Claims (11)
1. a kind of biomembrane anti-permeability detection means, it is characterised in that it is impermeable that it is applied to the low material of low-density, resistance to hydrostatic pressure value
The detection of performance.Its structure includes kerve, U-tube, fixture and the part of straight tube four.Kerve is the envelope with " convex " type opening
Tetragonal body is closed, openings of sizes matches with U-tube.Fixed column is provided with opening two ends, is easy to fix U-tube, and protect it
Water holding is put down;The baffle plate of kerve surrounding, to prevent unrestrained table top during liquid in containing liquid or experiment completion discharge straight tube and U-tube.
The frosted platform not damaged fixed biofilm of U-tube and straight tube, the piston control flow rate of liquid of U-tube, realizes hydrodynamics liquid
Body conducts pressure.The horizontal bottom of the tube of U-tube is provided with discharge outlet, by rubber hose directly with one of discharge outlet of kerve
Connection.
2. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:A length of 20~60cm, it is a width of
Directly over the kerve of 20~40cm, a height of 5~10cm, " convex " type opening is provided with 5~20cm of middle position, its
Size is 1~1.5 times of U-tube.
3. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:Kerve " convex " type opening two
End is provided with fixed column, and pillar height is 5~50cm.
4. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:Kerve surrounding height of baffle plate is
2~10cm.
5. a kind of biomembrane barrier type detection means according to claim 1, it is characterised in that:Kerve two ends are provided with 2 water outlets
Mouthful, respectively apart from base 0.5~1cm and 1~3cm;In the rubber hose and U-tube that latter of which passes through a diameter of 0.6~1cm
Discharge outlet connection, and its level height 0.5~2cm low compared with U-tube discharge outlet.
6. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:U-tube internal diameter is 1-20cm.
7. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:U-tube is highly
10-100cm。
8. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:The frosted of U-tube and straight tube
Berm width is 0.5~5cm.
9. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:U-tube frosted platform distance
2~10cm of kerve.
10. a kind of biomembrane anti-permeability detection means according to claim 1, it is characterised in that:U-tube is provided with 1 work
Plug, 2~10cm of plunger distance kerve.
A kind of 11. biomembrane anti-permeability detection means according to claim 9 and 10, it is characterised in that:The frosted of U-tube
Platform and piston in same level highly.
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CN201510929702.2A CN106885764B (en) | 2015-12-16 | 2015-12-16 | A kind of biomembrane anti-permeability detection device and application |
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CN201510929702.2A CN106885764B (en) | 2015-12-16 | 2015-12-16 | A kind of biomembrane anti-permeability detection device and application |
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CN106885764B CN106885764B (en) | 2019-08-27 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110243691A (en) * | 2019-06-25 | 2019-09-17 | 山东省水利科学研究院 | A kind of test macro and method of antiseepage composite geo-membrane |
CN112649293A (en) * | 2020-11-05 | 2021-04-13 | 华北水利水电大学 | Testing arrangement of geotechnical membrane hydraulic characteristic decay under big deformation state |
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CN112649293A (en) * | 2020-11-05 | 2021-04-13 | 华北水利水电大学 | Testing arrangement of geotechnical membrane hydraulic characteristic decay under big deformation state |
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