CN106883849A - Graphene quantum dot that a kind of nitrogenous sulphur mixes and preparation method thereof and the application on lysine luciferase assay reagent is prepared - Google Patents
Graphene quantum dot that a kind of nitrogenous sulphur mixes and preparation method thereof and the application on lysine luciferase assay reagent is prepared Download PDFInfo
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- CN106883849A CN106883849A CN201710195330.4A CN201710195330A CN106883849A CN 106883849 A CN106883849 A CN 106883849A CN 201710195330 A CN201710195330 A CN 201710195330A CN 106883849 A CN106883849 A CN 106883849A
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- quantum dot
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Abstract
Graphene quantum dot that a kind of nitrogenous sulphur mixes and preparation method thereof and the application on lysine luciferase assay reagent is prepared, the graphene quantum dot mixed using the nitrogenous sulphur of the product of thiocarbamide and citric acid water thermal response, the nano-quantum point has polyelectron characteristic, good water dispersible, with outstanding biocompatibility, rich in oxygen-containing functional group (COOH, OH, O), the strong and high quantum efficiency of photism.There is selectivity and sensitivity high to lysine.Its synthetic method is simple, mild condition, product are easy to get, lysine is tested using the method for the present invention, amino acid and carbohydrate biomolecule is not coexisted by other routines, such as influence of the material such as alanine, proline, glycine, methionine, arginine, leucine and glucose, Glucosamine, galactolipin, sucrose, with selectivity high.
Description
Technical field
Combined and for the technical field of fluorescence detection of optical detection lysine the present invention relates to recognize, and in particular to a kind of
Graphene quantum dot that nitrogenous sulphur mixes and preparation method thereof and the application on lysine luciferase assay reagent is prepared.
Background technology
Lysine is a kind of essential amino acid, is widely used in medicine, food processing and agricultural.It has numerous lifes
Reason function, such as participates in the synthesis and Krebs-Henseleit circulations of polyamines, and lysine diet is unbalance and some congenital generations
Thank to disease the same, cystinuria or hyperlysinemia can be caused.Traditional detection method is such as：High performance liquid chromatography
(HPLC), Capillary Electrophoresis (CE), Flow Injection Chemiluminescence and chromatography.But these methods are required for complexity
The personnel of pretreatment, cost high and specialty.Recognize that lysine carries out context of detection and achieves larger to enter with organic molecule
Exhibition, wherein, fluorimetry sensitivity test high is easy.But, due to being used to recognize the small molecule of lysine in these methods
Dissolubility is low in water mostly or causes the secondary pollution of environment and corresponding slower development.
The content of the invention
To solve the problems, such as the existing cumbersome inconvenience of lysine detection, the invention provides the graphite that a kind of nitrogenous sulphur mixes
Alkene quantum dot and preparation method thereof and the application in lysine fluoroscopic examination.
The present invention use technical solution be：The graphene quantum dot that a kind of nitrogenous sulphur mixes, its molecular structural formula
It is as follows：
。
A kind of preparation method of the graphene quantum dot that nitrogenous sulphur mixes, comprises the following steps：In citric acid and thiocarbamide
The ionized water of 5-35 mL is added, is stirred, mixed solution is transferred to heating response, the product obtained by reaction in reactor
Thing is dialysed three days with bag filter, obtains final product the graphene quantum dot that described nitrogenous sulphur mixes.
Described citric acid and the mass ratio of thiocarbamide are 1:0.2-1:3.
Heating response temperature is 130 ~ 230 DEG C in described reactor.
The heating response time is 2 ~ 20 h in described reactor.
A kind of application of the graphene quantum dot that nitrogenous sulphur mixes on lysine luciferase assay reagent is prepared.
Described lysine luciferase assay reagent is prepared by the following method：Nitrogenous sulphur described in claim 1 is mixed
Graphene quantum dot, be dissolved in water or alcohol solution, be made into graphene quantum dot mass concentration that nitrogenous sulphur mixes for 0.02 ~
The detection reagent solution of 5.0 mg/mL.
The beneficial effect that the present invention is obtained is：The graphene quantum dot and its system mixed the invention provides a kind of nitrogenous sulphur
Preparation Method and the application on lysine luciferase assay reagent is prepared, using the nitrogenous sulphur of the product of thiocarbamide and citric acid water thermal response
The graphene quantum dot for mixing, the nano-quantum point has polyelectron characteristic, good water dispersible, with outstanding biofacies
Capacitive, rich in oxygen-containing functional group (- COOH ,-OH ,-O-), the strong and high quantum efficiency of photism.There is height to lysine
Selectivity and sensitivity.Its synthetic method is simple, mild condition, product are easy to get, and lysine is tested using the method for the present invention,
Amino acid and carbohydrate biomolecule, such as alanine, proline, glycine, methionine, smart ammonia is not coexisted by other routines
The influence of the material such as sour, leucine and glucose, Glucosamine, galactolipin, sucrose, with selectivity high.
Brief description of the drawings
Fig. 1 is corresponding to the fluorescence of different lysine concentrations for the graphene quantum dot that the nitrogen sulphur of the embodiment of the present invention 1 mixes
Figure.
Fig. 2 for the embodiment of the present invention 2 the graphene quantum dot that mixes of nitrogen sulphur in the presence of 4 times of interfering ions to lysine
Fluorescence accordingly scheme.
Specific embodiment：
A kind of graphene quantum dot that nitrogenous sulphur mixes, molecular structural formula is as follows：
。
The preparation method of the graphene quantum dot that nitrogenous sulphur mixes, preparation method is as follows：Citric acid is added in three-necked bottle
And thiocarbamide, the mL of appropriate amount of deionized water 5 ~ 35 is added, stir into uniform solution.Mixed solution is transferred to polytetrafluoroethylene (PTFE) inner sleeve
In water heating kettle.The mass ratio of citric acid and thiocarbamide is 1:0.2~1:3, the heat time is 2 ~ 20 h, and heating-up temperature is 130 ~ 230
℃.Resulting product molecular weight is that 1000 bag filter is dialysed three days, obtains final product the Graphene amount that described nitrogenous sulphur mixes
Sub- point.
Prepare lysine luciferase assay reagent：The graphene quantum dot that nitrogenous sulphur is mixed, is dissolved in water or alcohol solution, water
Solution is more practical and easy to use, is made into finite concentration.Concentration can be carried out according to test condition and fluorescence intensity change degree
Determine, for the graphene quantum dot that nitrogenous sulphur of the invention mixes, mass concentration can be made into for 0.02 ~ 5.0 mg/mL.Contain
Test concentrations after the sample addition of lysine are 10-5 M~4*10-3 M is more preferable.Specific test is surveyed using fluorimetry
Examination, content reckoning is carried out using standard working curve method.
Embodiment 1
The graphene quantum dot that nitrogen sulphur mixes in fluorescence experiments is made into 1.0 mg/mL aqueous solution storing solutions, amino acid and carbohydrate point
Son is from lysine, alanine, proline, glycine, methionine, arginine, leucine and glucose, aminoglucose
The materials such as sugar, galactolipin, sucrose, the solution of all experiments is all new configuration, and is tested immediately.Excited in 340 nm, 440
Nm launches.The mL of storing solution 2.5 is taken, 2.5*10 is separately added into-2 The amino acid or saccharide solution of M.Test its fluorescence spectrum.As a result
See accompanying drawing 1.
Embodiment 2
The graphene quantum dot that nitrogen sulphur mixes in fluorescence experiments is made into the aqueous solution of 0.04 mg/mL.Lysine is made into 2.5*10-2 The standard reserving solution of M.As interference amino acid and carbohydrate molecule from alanine, proline, glycine, methionine,
The material such as arginine, leucine and glucose, Glucosamine, galactolipin, sucrose.The solution of all experiments is all newly to match somebody with somebody
Put, and test immediately.In interfering material experiment, first in the aqueous solution of the graphene quantum dot that the nitrogen sulphur of 0.04 mg/mL mixes
4 times of interfering ion is added, its fluorescence is surveyed, lysine is added, its change in fluorescence is surveyed.Excited in 340 nm, at 440 nm
Detection change in fluorescence.In Fig. 2 in every group, bar-shaped mark is low corresponding for interfering ion, and high is corresponding after lysine to add.
Specific embodiment is served only for being further described the present invention, it is impossible to used as the limit to the scope of the present invention
It is fixed, while person skilled in art makes some nonessential modifications and adaptations to the present invention according to the content of foregoing invention,
It is all located in protection scope of the present invention, protection scope of the present invention is defined by claims.
Claims (7)
1. the graphene quantum dot that a kind of nitrogenous sulphur mixes, it is characterised in that：Its molecular structural formula is as follows：
。
2. the preparation method of the graphene quantum dot that the nitrogenous sulphur described in a kind of claim 1 mixes, it is characterised in that：Including with
Lower step：The ionized water of 5-35 mL is added in citric acid and thiocarbamide, is stirred, mixed solution is transferred in reactor
Heating response, the product obtained by reaction is dialysed three days with bag filter, obtains final product the graphene quantum dot that described nitrogenous sulphur mixes.
3. the preparation method of the graphene quantum dot that nitrogenous sulphur according to claim 2 mixes, it is characterised in that：Described
The mass ratio of citric acid and thiocarbamide is 1:0.2-1:3.
4. the preparation method of the graphene quantum dot that nitrogenous sulphur according to claim 2 mixes, it is characterised in that：Described
Heating response temperature is 130 ~ 230 DEG C in reactor.
5. the preparation method of the graphene quantum dot that nitrogenous sulphur according to claim 2 mixes, it is characterised in that：Described
The heating response time is 2 ~ 20 h in reactor.
6. the graphene quantum dot that the nitrogenous sulphur described in a kind of claim 1 mixes is on lysine luciferase assay reagent is prepared
Using.
7. the graphene quantum dot that nitrogenous sulphur according to claim 6 mixes is on lysine luciferase assay reagent is prepared
Using, it is characterised in that：Described lysine luciferase assay reagent is prepared by the following method：By containing described in claim 1
The graphene quantum dot that nitrogen sulphur mixes, is dissolved in water or alcohol solution, is made into the graphene quantum dot mass concentration that nitrogenous sulphur mixes
It is the detection reagent solution of 0.02 ~ 5.0 mg/mL.
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| CN107941768A (en) * | 2017-11-29 | 2018-04-20 | 温州医科大学 | A kind of applications of glucosan derivative RG on histidine luciferase assay reagent is prepared |
| CN108328607A (en) * | 2018-02-12 | 2018-07-27 | 吉林师范大学 | Nitrogen, the chlorine of a kind of double transmittings of super-small blue green light are co-doped with graphene quantum dot and preparation method thereof |
| CN108359454A (en) * | 2018-02-08 | 2018-08-03 | 中国科学院苏州生物医学工程技术研究所 | Carbon nano dot with red emission, preparation method and applications |
| CN108503921A (en) * | 2018-05-23 | 2018-09-07 | 江南大学 | A kind of preparation method of thiocarbamide functionalized nano Graphene antibiosis material |
| CN108609617A (en) * | 2018-05-25 | 2018-10-02 | 温州医科大学 | Graphene quantum dot GSG that a kind of polypeptide is modified and preparation method thereof with prepare the application on lysine luciferase assay reagent |
| CN108822839A (en) * | 2018-05-25 | 2018-11-16 | 温州医科大学 | Modified nanometer carbon dots GSCs of a kind of Glucosamine and preparation method thereof with prepare the application on lysine luciferase assay reagent |
| CN109181416A (en) * | 2018-08-10 | 2019-01-11 | 淄博职业学院 | A kind of industrial and commercial administration Antiforge fluorescent ink and preparation method thereof |
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| CN110061252A (en) * | 2019-05-08 | 2019-07-26 | 安康学院 | A kind of fuel battery negative pole oxygen reduction catalyst nitrogen sulphur codope graphene/carbon point composite material and preparation method and application |
| CN110155992A (en) * | 2019-06-14 | 2019-08-23 | 福州大学 | A kind of preparation method of the sulfur and nitrogen co-doped graphene quantum dot electrolyte suitable for supercapacitor |
| CN110980689A (en) * | 2019-12-19 | 2020-04-10 | 封静义 | Fluorescent probe for specifically detecting thyroid hormone and application thereof |
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| CN115161019A (en) * | 2022-05-11 | 2022-10-11 | 华中农业大学 | Preparation method of nitrogen-doped luminescent carbon quantum dot and application of nitrogen-doped luminescent carbon quantum dot in rapid detection of lysine content in pig serum |
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| CN107500274A (en) * | 2017-10-11 | 2017-12-22 | 江苏安纳泰环保科技有限公司 | A kind of preparation method of the graphene quantum dot of three primary colors fluorescence |
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| CN108359454A (en) * | 2018-02-08 | 2018-08-03 | 中国科学院苏州生物医学工程技术研究所 | Carbon nano dot with red emission, preparation method and applications |
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| CN108328607B (en) * | 2018-02-12 | 2021-05-11 | 吉林师范大学 | Ultra-small blue-green light dual-emission nitrogen and chlorine co-doped graphene quantum dot and preparation method thereof |
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