CN106868090B - Sputum treatment method for medical examination - Google Patents
Sputum treatment method for medical examination Download PDFInfo
- Publication number
- CN106868090B CN106868090B CN201710192909.5A CN201710192909A CN106868090B CN 106868090 B CN106868090 B CN 106868090B CN 201710192909 A CN201710192909 A CN 201710192909A CN 106868090 B CN106868090 B CN 106868090B
- Authority
- CN
- China
- Prior art keywords
- parts
- treatment
- sputum
- prepared
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a method for treating sputum for medical examination, which comprises the following steps: (1) adding the sputum specimen into the first treatment solution, and carrying out intermittent ultrasonic treatment at 0-5 ℃; (2) adding a second treatment solution, and carrying out intermittent ultrasonic treatment at 20-30 ℃; (3) smearing, dyeing and sealing; the first treatment liquid is prepared from the following components in parts by weight: 0.3-0.4 part of 2-mercaptoethanol, 0.6-0.8 part of lauric acid monoethanolamide sodium sulfate, 12-18 parts of potassium chloride, 1-2 parts of monopotassium phosphate, 8-10 parts of dipotassium hydrogen phosphate and 1500-1800 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1-2 parts of farrerol, 0.3-0.6 part of cynanchum glaucescens and 50-60 parts of ethanol. The treatment method disclosed by the invention not only realizes homogenization of the sputum specimen, but also protects bacteria in the sputum specimen from being damaged as much as possible, and has better clinical medical examination significance.
Description
Technical Field
The invention relates to a sputum treatment method, in particular to a sputum treatment method for medical examination. Belongs to the technical field of medical examination.
Background
The sputum specimen collection is a common means in medical examination, and there are two conventional methods for obtaining the sputum specimen, namely a natural expectoration method or a sputum aspiration method, and the sputum specimen obtained by the two methods has poor homogeneity, wherein the distribution of bacteria and the like is also very uneven, and objective and accurate examination results cannot be obtained naturally by direct examination, so that the sputum specimen is required to be pretreated before the medical examination.
Currently, commercial sputum digestants are generally used to treat sputum specimens, such as Sputasol SR0233, manufactured by OXOID, united kingdom, which contains dithiothreitol, sodium chloride, potassium chloride, disodium hydrogen phosphate, and the like as main components. In addition, some hospitals use trypsin or chymotrypsin to digest sputum.
However, there are two important treatment points for sputum specimen, one is homogenization and the other is to protect bacteria in the sputum specimen from being damaged, and the current sputum digestive juice product cannot satisfy the two points at the same time, and still has a great space for improvement.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for treating sputum for medical examination.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into a first treatment solution with the weight being 2-3 times that of the sputum specimen, and carrying out intermittent ultrasonic treatment at 0-5 ℃ for 2-3 minutes;
(2) adding 0.5-1 time of the second treatment liquid by weight, and carrying out intermittent ultrasonic treatment at 20-30 ℃ for 1-2 minutes;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.3-0.4 part of 2-mercaptoethanol, 0.6-0.8 part of lauric acid monoethanolamide sodium sulfate, 12-18 parts of potassium chloride, 1-2 parts of monopotassium phosphate, 8-10 parts of dipotassium hydrogen phosphate and 1500-1800 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1-2 parts of farrerol, 0.3-0.6 part of cynanchum glaucescens and 50-60 parts of ethanol; the intermittent ultrasonic treatment method comprises the step of treating once every 1 minute, wherein the treatment time is 20-30 seconds each time.
Preferably, the first treatment liquid is prepared by dissolving the formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding the formula amount of 2-mercaptoethanol, and stirring for uniformly mixing.
Preferably, the second treatment solution is prepared by adding farrerol and cynanchum glaucescens in a formula amount into ethanol, heating and refluxing for 2-3 hours, and filtering to obtain a filtrate.
Preferably, in the step (3), the staining is selected from hematoxylin-eosin staining or papanicolaou staining.
The invention has the beneficial effects that:
the treatment method disclosed by the invention not only realizes homogenization of the sputum specimen, but also protects bacteria in the sputum specimen from being damaged as much as possible, and has better clinical medical examination significance.
The viscous substance of the sputum is mainly glycoprotein combined by disulfide bond crosslinking, the disulfide bond is reduced to SH & lt- & gt by using 2-mercaptoethanol, but SH & lt- & gt is easily oxidized in the air and then forms a disulfide bond again, micelle protection is further formed by lauric acid monoethanolamide sodium sulfate so as to prevent re-crosslinking, and most of glycoprotein in the sputum specimen is decomposed by a reduction method.
Farrerol and cynanchum glaucescens can break the fiber of the remaining glycoprotein in the sputum, reduce the sialic acid content, reduce the viscosity of the sputum, and protect cells from being damaged.
The sputum is not transferred again in the treatment process, so that partial component loss possibly caused by the retransfer is avoided, and further, the detection result is inaccurate.
In addition, it is worth noting that the ultrasonic wave has a cavitation effect, and can generate a large amount of high-pressure and high-temperature bubbles, and the cell walls of the bacteria can be destroyed only after a period of time. The invention utilizes the resonance effect of ultrasonic waves to realize homogenization, and avoids the occurrence of cavitation by controlling intermittent ultrasonic treatment.
Detailed Description
The present invention will be further illustrated by the following examples, which are intended to be merely illustrative and not limitative.
Example 1:
a method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into 2 times of the first treatment solution by weight, and carrying out intermittent ultrasonic treatment at 0 ℃ for 2 minutes;
(2) adding 0.5 times of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment at 20 ℃ for 1 minute;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.3 part of 2-mercaptoethanol, 0.6 part of lauric acid monoethanolamide sodium sulfate, 12 parts of potassium chloride, 1 part of potassium dihydrogen phosphate, 8 parts of dipotassium hydrogen phosphate and 1500 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1 part of farrerol, 0.3 part of cynanchum glaucescens and 50 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 20 seconds each time.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 2 hr, filtering, and collecting filtrate.
In the step (3), the staining is hematoxylin-eosin staining.
Example 2:
a method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into 3 times of the first treatment solution by weight, and carrying out intermittent ultrasonic treatment for 3 minutes at 5 ℃;
(2) adding 1 time of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment for 2 minutes at 30 ℃;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.4 part of 2-mercaptoethanol, 0.8 part of lauric acid monoethanolamide sodium sulfate, 18 parts of potassium chloride, 2 parts of potassium dihydrogen phosphate, 10 parts of dipotassium hydrogen phosphate and 1800 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 2 parts of farrerol, 0.6 part of cynanchum glaucescens and 60 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 30 seconds each time.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 3 hr, filtering, and collecting filtrate.
In the step (3), the staining is Papanicolaou staining.
Example 3:
a method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into 2 times of the first treatment solution by weight, and carrying out intermittent ultrasonic treatment for 2 minutes at 5 ℃;
(2) adding 1 time of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment for 2 minutes at 20 ℃;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.3 part of 2-mercaptoethanol, 0.8 part of sodium laureth anolamide sulfate, 12 parts of potassium chloride, 2 parts of potassium dihydrogen phosphate, 8 parts of dipotassium hydrogen phosphate and 1800 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1 part of farrerol, 0.6 part of cynanchum glaucescens and 50 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 30 seconds each time.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 2 hr, filtering, and collecting filtrate.
In the step (3), the staining is Papanicolaou staining.
Example 4:
a method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into 3 times of the first treatment solution by weight, and carrying out intermittent ultrasonic treatment at 0 ℃ for 3 minutes;
(2) adding 0.5 times of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment at 30 ℃ for 1 minute;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.4 part of 2-mercaptoethanol, 0.6 part of lauric acid monoethanolamide sodium sulfate, 18 parts of potassium chloride, 1 part of potassium dihydrogen phosphate, 10 parts of dipotassium hydrogen phosphate and 1500 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 2 parts of farrerol, 0.3 part of cynanchum glaucescens and 60 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 20 seconds each time.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 3 hr, filtering, and collecting filtrate.
In the step (3), the staining is hematoxylin-eosin staining.
Example 5:
a method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into a first treatment solution with the weight 2.5 times that of the sputum specimen, and carrying out intermittent ultrasonic treatment at the temperature of 2 ℃ for 2.5 minutes;
(2) adding 0.8 times of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment at 25 ℃ for 1.5 minutes;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.35 part of 2-mercaptoethanol, 0.7 part of lauric acid monoethanolamide sodium sulfate, 15 parts of potassium chloride, 1.5 parts of potassium dihydrogen phosphate, 9 parts of dipotassium hydrogen phosphate and 1600 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1.5 parts of farrerol, 0.5 part of cynanchum glaucescens and 55 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 25 seconds each time.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 2.5 hr, and filtering to obtain filtrate.
In the step (3), the staining is Papanicolaou staining.
Comparative example 1
A method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into a first treatment solution with the weight 2.5 times that of the sputum specimen, and carrying out intermittent ultrasonic treatment at the temperature of 2 ℃ for 2.5 minutes;
(2) adding 0.8 times of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment at 25 ℃ for 1.5 minutes;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.35 part of 2-mercaptoethanol, 15 parts of potassium chloride, 1.5 parts of monopotassium phosphate, 9 parts of dipotassium hydrogen phosphate and 1600 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1.5 parts of farrerol, 0.5 part of cynanchum glaucescens and 55 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 25 seconds each time.
The first treating liquid is prepared through dissolving potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride in distilled water, stirring to dissolve, adding 2-mercaptoethanol, and stirring.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 2.5 hr, and filtering to obtain filtrate.
In the step (3), the staining is Papanicolaou staining.
Comparative example 2
A method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into a first treatment solution with the weight 2.5 times that of the sputum specimen, and carrying out intermittent ultrasonic treatment at the temperature of 2 ℃ for 2.5 minutes;
(2) adding 0.8 times of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment at 25 ℃ for 1.5 minutes;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.35 part of 2-mercaptoethanol, 0.7 part of lauric acid monoethanolamide sodium sulfate, 15 parts of potassium chloride, 1.5 parts of potassium dihydrogen phosphate, 9 parts of dipotassium hydrogen phosphate and 1600 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1.5 parts of farrerol and 55 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 25 seconds each time.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 2.5 hr, and filtering to obtain filtrate.
In the step (3), the staining is Papanicolaou staining.
Comparative example 3
A method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into 2.5 times of the first treatment solution by weight, and continuously carrying out ultrasonic treatment for 2.5 minutes at 2 ℃;
(2) adding 0.8 times of the second treatment solution by weight, and continuously carrying out ultrasonic treatment for 1.5 minutes at 25 ℃;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.35 part of 2-mercaptoethanol, 0.7 part of lauric acid monoethanolamide sodium sulfate, 15 parts of potassium chloride, 1.5 parts of potassium dihydrogen phosphate, 9 parts of dipotassium hydrogen phosphate and 1600 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1.5 parts of farrerol, 0.5 part of cynanchum glaucescens and 55 parts of ethanol.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 2.5 hr, and filtering to obtain filtrate.
In the step (3), the staining is Papanicolaou staining.
Comparative example 4
A method for treating sputum for medical examination comprises the following steps:
(1) adding the sputum specimen into 0.8 times of the second treatment solution by weight, and carrying out intermittent ultrasonic treatment for 1.5 minutes at 25 ℃;
(2) adding 2.5 times of the first treatment solution by weight, and carrying out intermittent ultrasonic treatment at 2 ℃ for 2.5 minutes;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.35 part of 2-mercaptoethanol, 0.7 part of lauric acid monoethanolamide sodium sulfate, 15 parts of potassium chloride, 1.5 parts of potassium dihydrogen phosphate, 9 parts of dipotassium hydrogen phosphate and 1600 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1.5 parts of farrerol, 0.5 part of cynanchum glaucescens and 55 parts of ethanol; the intermittent ultrasonic treatment method is to treat every 1 minute, and the treatment time is 25 seconds each time.
The first treatment liquid is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, stirring and mixing well to obtain the final product.
The second treatment solution is prepared by adding farrerol and rhizoma Cynanchi Stauntonii in ethanol, heating and refluxing for 2.5 hr, and filtering to obtain filtrate.
In the step (3), the staining is Papanicolaou staining.
Test examples
Respectively treating 100 parts of sputum samples by the methods of examples 1-5 or comparative examples 1-4, inoculating the sputum samples treated in step (2) on a blood plate, and placing in CO at 35 deg.C2IncubatorThe culture is carried out for 3 days, the bacterial species are distinguished through the colony morphology and simplification, the number of the bacterial species cultured on the blood plate by the sputum specimen treated by different methods is recorded, and the results are shown in table 1.
TABLE 1 statistics of the number of species in sputum specimens processed by different methods
As can be seen from Table 1, the sputum specimens of examples 1 to 5 were homogenized, and the number of bacterial species in each fraction was relatively close, and about 80% of the samples were in the same interval (3 types). Comparative example 1 no sodium laureth sulfate was added, and comparative example 2 omitted the blank time, and their homogenization was significantly deteriorated and the distribution was observed in each interval. Comparative example 3, in which continuous ultrasonic treatment was used, and comparative example 4, in which the treatment steps of the first treatment liquid and the second treatment liquid were reversed, the damage to the bacteria was significantly large, there was no distribution in the interval of more than 3 species, the distribution in the interval of 3 species was also small, and most of them was concentrated in the two intervals of 1 species and 2 species.
100 patients diagnosed with lung adenocarcinoma by CT are treated with sputum samples according to the methods of examples 1-5 or comparative examples 1-4, and then subjected to medical examination, and the results are shown in Table 2.
TABLE 2 statistics of medical test Positive Rate
As can be seen from Table 2, the sputum test positive rate of the lung adenocarcinoma patients is more than 50%, while the clinical sputum test false negative rate is very high (the positive rate is detected to be about 15%), so that the false negative rate is obviously reduced. The positive rate of the comparative examples 1 to 4 is significantly low.
Although the present invention has been described with reference to the specific embodiments, it is not intended to limit the scope of the present invention, and various modifications and variations can be made by those skilled in the art without inventive changes based on the technical solution of the present invention.
Claims (2)
1. A sputum treatment method for medical examination is characterized by comprising the following specific steps:
(1) adding the sputum specimen into a first treatment solution with the weight being 2-3 times that of the sputum specimen, and carrying out intermittent ultrasonic treatment at 0-5 ℃ for 2-3 minutes;
(2) adding 0.5-1 time of the second treatment liquid by weight, and carrying out intermittent ultrasonic treatment at 20-30 ℃ for 1-2 minutes;
(3) smearing, dyeing and sealing;
the first treatment liquid is prepared from the following components in parts by weight: 0.3-0.4 part of 2-mercaptoethanol, 0.6-0.8 part of lauric acid monoethanolamide sodium sulfate, 12-18 parts of potassium chloride, 1-2 parts of monopotassium phosphate, 8-10 parts of dipotassium hydrogen phosphate and 1500-1800 parts of distilled water; the second treatment liquid is prepared from the following components in parts by weight: 1-2 parts of farrerol, 0.3-0.6 part of cynanchum glaucescens and 50-60 parts of ethanol; the intermittent ultrasonic treatment method comprises the steps of treating every 1 minute for 20-30 seconds;
the first treatment solution is prepared by dissolving formula amount of lauric acid monoethanolamide sodium sulfate in distilled water, sequentially adding potassium dihydrogen phosphate, dipotassium hydrogen phosphate and potassium chloride, stirring for dissolving, finally adding formula amount of 2-mercaptoethanol, and stirring for mixing uniformly;
the second treatment solution is prepared by adding farrerol and cynanchum glaucescens in a formula amount into ethanol, heating and refluxing for 2-3 hours, and filtering to obtain a filtrate.
2. The method for treating sputum for medical examination according to claim 1, wherein in step (3), the staining is selected from hematoxylin-eosin staining or papanicolaou staining.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710192909.5A CN106868090B (en) | 2017-03-28 | 2017-03-28 | Sputum treatment method for medical examination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710192909.5A CN106868090B (en) | 2017-03-28 | 2017-03-28 | Sputum treatment method for medical examination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106868090A CN106868090A (en) | 2017-06-20 |
| CN106868090B true CN106868090B (en) | 2020-09-29 |
Family
ID=59160489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710192909.5A Active CN106868090B (en) | 2017-03-28 | 2017-03-28 | Sputum treatment method for medical examination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106868090B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114235524B (en) * | 2021-12-10 | 2024-02-23 | 南昌准好生物科技有限公司 | Sputum treatment method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002044691A2 (en) * | 2000-11-28 | 2002-06-06 | Mcmaster University | Sputum fixative and methods and uses therefor |
| CN103555809A (en) * | 2013-10-11 | 2014-02-05 | 上海微银生物技术有限公司 | Mycobacterium tuberculosis sputum specimen liquefier kit and application thereof |
| CN103884549A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Sputum cell treatment liquid and application thereof |
| CN104561230A (en) * | 2015-01-22 | 2015-04-29 | 四川金域医学检验中心有限公司 | Sputum specimen homogenization method and preparation of reagent for medical detection of sputum specimen |
| CN105188738A (en) * | 2013-03-15 | 2015-12-23 | 奥普罗治疗学股份有限公司 | Product and process for mucus viscosity normalization |
-
2017
- 2017-03-28 CN CN201710192909.5A patent/CN106868090B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002044691A2 (en) * | 2000-11-28 | 2002-06-06 | Mcmaster University | Sputum fixative and methods and uses therefor |
| CN105188738A (en) * | 2013-03-15 | 2015-12-23 | 奥普罗治疗学股份有限公司 | Product and process for mucus viscosity normalization |
| CN103555809A (en) * | 2013-10-11 | 2014-02-05 | 上海微银生物技术有限公司 | Mycobacterium tuberculosis sputum specimen liquefier kit and application thereof |
| CN103884549A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Sputum cell treatment liquid and application thereof |
| CN104561230A (en) * | 2015-01-22 | 2015-04-29 | 四川金域医学检验中心有限公司 | Sputum specimen homogenization method and preparation of reagent for medical detection of sputum specimen |
Non-Patent Citations (3)
| Title |
|---|
| Measures of gene expression in sputum cells can identify TH2-high and TH2-low subtypes of asthma;Michael C. Peters et al.;《J Allergy Clin Immunol》;20140228;第133卷(第2期);第388-394页 * |
| 痰标本前处理对细菌培养结果的影响;叶肖燕;《临床医学工程》;20110831;第18卷(第8期);第1177-1178页 * |
| 祛痰药的临床应用评价;王春等;《中国医院用药评价与分析》;20061231;第6卷(第4期);第209页第2.3（4）节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106868090A (en) | 2017-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Royce et al. | Reduced lysyl oxidase activity in skin fibroblasts from patients with Menkes' syndrome | |
| CN1171991C (en) | Culture process of human nerve stem cell | |
| Norkrans | Influence of Cellulolytic Enzymes from Hymenomycetes on Cellulose Preparations of Different Crystallinity. | |
| Castañeda-Agulló | Studies on the biosynthesis of extracellular proteases by bacteria: I. Serratia marcescens. synthetic and gelatin media | |
| Bell et al. | Observations on the chemistry of liver glycogen | |
| CN105779388B (en) | A kind of culture medium and its cultural method of umbilical cord blood mesenchymal stem cells | |
| CN106868090B (en) | Sputum treatment method for medical examination | |
| CN108095129B (en) | Method for preparing water-soluble dietary fibers of bran through fermentation | |
| CN106867981A (en) | A kind of human umbilical tissue digestive ferment and preparation method thereof | |
| CN114958823A (en) | Method for producing extracellular polysaccharide by using static magnetic field to assist fermentation of paenibacillus polymyxa | |
| LU506863B1 (en) | Method for inactivating virus and application | |
| CN109439621A (en) | A kind of mild enzyme cell dissociation buffer of stem cell | |
| JPS6034181A (en) | Preparation of neuraminidase | |
| CN107236705A (en) | A kind of intermembranous mesenchymal stem cells cultivating system of human placenia | |
| EP0630401B1 (en) | Strain w micro-organism, enzyme compositions obtainable therefrom, use of the micro-organism and hydrolysis process especially of keratin | |
| CN106834259A (en) | A kind of human fat tissue digestive ferment and preparation method thereof | |
| CN107446986A (en) | Culture medium of microorganism and preparation method thereof in a kind of detection blood of human body | |
| CN107828724A (en) | It is a kind of to be used to cultivate culture medium of fat stem cell and preparation method thereof | |
| CN114480195B (en) | Quick helicobacter pylori enrichment culture reagent and preparation method thereof | |
| CN112266909B (en) | Viscous biological sample digestant and preparation method thereof | |
| CN106834213B (en) | Induction medium and induction method for inducing pluripotent stem cells to form hair papilla cells | |
| CN104711304A (en) | Method for producing germ nano-crystalline cellulose by utilizing tea fungus and raffinose | |
| CN112574938A (en) | Sputum treatment fluid for membrane filtration enriched bacteria and application thereof | |
| CN117486402A (en) | Sputum treatment method for medical examination | |
| Lasfargues et al. | Influence of human cord extracts on chick embryonic fibroblasts grown in vitro |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230606 Address after: No. 13 # -14 # -15 # (Jiangning High tech Park), Phase III, Jiangning Hi tech International Enterprise Port, Liandong U Valley, No. 7, Tonglian Road, Jiangning District, Nanjing, Jiangsu 211100 Patentee after: NANJING HUAYIN MEDICAL EXAMINATION LAB CO.,LTD. Address before: 262300 Wulian People's Hospital of Shandong, Rizhao City Province, China Patentee before: Wang Xuyou |