CN106858042A - A kind of pretreated corn pulp and its direct-injection application method in animal and fowl fodder - Google Patents
A kind of pretreated corn pulp and its direct-injection application method in animal and fowl fodder Download PDFInfo
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Abstract
A kind of direct-injection application method the invention discloses pretreated corn pulp and its in animal and fowl fodder, while corn pulp moisture is effectively reduced, by poisonous, ANFs the effective removal in corn pulp, enables to be directly used in feeding livestock and poultry.Specifically, the preprocess method of the corn pulp, comprises the following steps：(1) to adding alkali lye in corn pulp, stir process, adjusting corn slurry slurries pH to 5.2 5.3；(2) to phytase is added in the corn pulp after regulation pH, treatment is stirred and evenly mixed；(3) being respectively connected to cultured bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) in the corn pulp after being digested to gained in step (2) carries out fermented and cultured；After culture terminates, that is, obtain corn pulp.
Description
Technical field
The invention belongs to feed production technology field, specially a kind of pretreated corn pulp and its in animal and fowl fodder
Direct-injection application method.
Background technology
Corn pulp is the Main By product that corn deep processing is obtained, and is corn by after sulfurous acid solution immersion, then through broken
It is broken separation and produce, it contains abundant soluble protein, auxin and some precursor substances, containing about 40%~50% solid
Material, taste is micro- salty.At present, Dried Corn Steep Liquor Powder has been widely used for antibiotic industry, vitamin, amino acid, enzyme preparation etc.
Fermentation industry, can also serve as the nutrients such as water-soluble plant albumen and water soluble vitamin supplement in biological fermentation process
Agent, such as Wei Jiaqian (Wei Jiaqian, Wang Zhiye, Qi Hongshan, wait corn pulps dextran-fermentation production in application [J] in
State brewages, and 2008,27 (19):One of nitrogen source using corn pulp as dextran-fermentation 64-66.) is reported, corn is have studied
Influence of the slurry addition to dextran, research shows, when corn pulp content is 0.1% in fermentation medium, thick dextrose
Acid anhydride yield highest；(Zhang Fengqing, Zhang Haiyue, Zheng Chunyu wait corn pulps to do Acetobacter xylinum culture mediums to Zhang Fengqing etc.
Produce fermentation condition optimization [J] the food industry science and technology of cellulose, 2005 (2):107-108.) report using corn pulp as
Acetobacter xylinum nutrition nitrogen source, explores the optimization formula of fermentation medium, and fermentation condition is carried out using single-factor optimization
Optimization.Result shows, glucose 4%, corn pulp 5.5%, disodium hydrogen phosphate 0.5%, biotin 25mg/L, caffeine 2mg/
L, citric acid 0.1%, pH is 6, and using the static shallow-layer cultivation of acetobacter xylinum, the yield of cellulose is up to 7.22g/L, compares yeast
The cellulose output that cream does nitrogen source improves 1 times.Because China's corn resources are abundant, a large amount of corn pulps can be produced in process
Liquid, therefore need badly and widen its application field, because corn pulp contains abundant nutriment, it has as the latent of animal and fowl fodder
In application value.
However, because unprocessed corn pulp water content is big, being unsuitable for being added directly in feed, convection drying is taken
Mode needs to consume mass energy, improves production cost.And due to also containing aspertoxin, phytate, sulfurous in corn pulp
Hydrochlorate etc. is poisonous or ANFs, such as without treatment added to efficiency of feed utilization, Er Qieke is not only greatly lowered in feed
Feeding livestock and poultry can be caused to be poisoned, be unfavorable for application of the corn pulp as feed ingredient.
The content of the invention
For above-mentioned prior art, the present invention provides a kind of pretreated corn pulp and its direct-injection in animal and fowl fodder
Application method, the corn pulp after pretreatment, can effectively reduce original corn pulp moisture, while by corn pulp
Poisonous, ANFs effective removal, enables to be directly used in livestock and poultry feeding.
Specifically, technical scheme is as follows：
A kind of pretreated corn pulp, the corn pulp water content is 6-10%, and sulfite content is less than 1%, glues
It is 50-80cps to spend；
Compared with original corn pulp, the present invention is through true albumen, Soluble phosphorus, total amino acid in the corn pulp that obtains for the treatment of and always must
Amino acid content is needed to be improved, palatability is obviously improved, comprehensive nutrient value is significantly improved, while present invention gained corn pulp
Water content is significantly reduced compared with before processing so that corn pulp raw material availability is greatly improved.
Direct-injection application method the invention also discloses the corn pulp in animal and fowl fodder, specifically, the direct-injection makes
It is that the addition of the corn pulp is 3-6% by corn pulp direct spraying input animal and fowl fodder with method.
By in corn pulp direct spraying input feed, the mixing of other components in animal and fowl fodder is not only improved, while
Because corn pulp contains certain viscosity, after direct-injection mode puts into feed, corn pulp also acts as the effect of binding agent, prevents from raising
Material nutrient material scatters and disappears, while experiment proves that feed feed consumption rate can be significantly reduced, and can reduce feed preparation
Dust, while so that feed is not pulverized easily in transportation.
Present invention also offers the specific preprocess method of above-mentioned corn pulp, the preprocess method comprises the following steps：
(1) to adding alkali lye in corn pulp, stir process, adjusting corn slurry slurries pH to 5.2-5.3；
(2) to phytase is added in the corn pulp after regulation pH, treatment is stirred and evenly mixed；
(3) cultured bacillus amyloliquefaciens is respectively connected in the corn pulp after being digested to gained in step (2)
(Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) carry out fermented and cultured；Culture terminates
Afterwards, that is, corn pulp is obtained.
Preferably, the alkali lye is NaOH solution, and the concentration of NaOH solution is 1mol/L；It is easy to regulation using NaOH solution
PH, while the pH after regulation is conducive to phytase fully to volatilize enzymatic activity, so that the phytic acid in efficient degradation corn pulp.
Preferably, the phytase is liquid phytase, and in terms of enzyme activity 5000U/ml, addition is 0.5~1.0L/m3
Meter；35~40 DEG C of temperature during phytase, rotating speed is 25~35r/min, and the stirring reaction time is 2~3h；Due to corn pulp
In phytic acid and divalence or Tricationic, protein etc. be complexed, insoluble salt is formed, so as to reduce its utilization rate, it is difficult to quilt
Livestock and poultry digest and absorb, therefore it is the key for improving corn pulp utilization rate to eliminate phytic acid ANFs.Due to corn pulp this
Shi Hanyou large quantity of moistures, phytase is fully mixed with corn pulp, the phytic acid in corn pulp can be effectively reduced, so as to be conducive to jade
Absorption of the Rice ＆ peanut milk in livestock and poultry.
Preferably, the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and aspergillus niger
The inoculation of (Aspergillus niger) is sequentially and the time is：First it is inoculated with bacillus amyloliquefaciens, speed of agitator 100~
150r/min, after 8~12h of aerobic fermentation, inoculates aspergillus niger, 100~150r/min of speed of agitator, 24~48h of aerobic fermentation；
Total inoculum concentration is 0.1~0.2% mass ratio, and wherein bacillus amyloliquefaciens and aspergillus niger mass ratio are 2~4：1, culture temperature
It is 30~35 DEG C to spend, 32~60h of total fermentation time；Corn pulp is obtained；
The bacillus amyloliquefaciens and aspergillus niger that the present invention is used are the Ministry of Agriculture《It is general on microbiological feed bio-safety
Technical criteria》Defined allows the production strain for using in annex, it is preferred that bacillus amyloliquefaciens bacterium numbering CICC
10074, aspergillus niger strain numbering is CICC 2106, is purchased from Chinese industrial Microbiological Culture Collection administrative center.Sent out through research
Existing, bacillus amyloliquefaciens and aspergillus niger can effectively remove AFB1 and zearalenone and its metabolin α-jade
The red mould enol of rice and ochratoxin A, while inventor has been surprisingly found that in experimental study, are first inoculated with bacillus amyloliquefaciens
After 24~48h of culture, aspergillus niger is inoculated, the two shows strong cooperative effect, in shorter fermentation time, for poison
The clearance of element is significantly improved；And by determining, after being processed through above-mentioned pre-treatment step, the soluble sulfurous in solution
Phosphate content is minimized, because remaining has the soluble sulphite (5~10%) of higher concentration in raw material corn pulp, although
Sulphite is widely used in food service industry as a kind of food additives, but tight because of its potential hazard
Lattice limit consumption, and after above-mentioned pre-treatment step, corn pulp sulfite salt content significantly reduces (content is less than 1%), and beautiful
Sulphates content in Rice ＆ peanut milk increases, therefore speculates to be probably to serve oxidation to sulphite in fermentation process to make
With so as to effectively reduce the sulfite content in corn pulp.
And, after being processed using above-mentioned preprocess method, true albumen, Soluble phosphorus, total amino acid and total required ammonia in corn pulp
Base acid content is improved, and palatability is obviously improved, and comprehensive nutrient value is significantly improved, while present invention gained corn pulp is aqueous
Amount is significantly reduced compared with before processing so that corn pulp raw material availability is greatly improved.
Beneficial effects of the present invention are：
(1) the pretreated corn pulp that the present invention is provided directly can be added in feed using direct-injection mode, reduced
The step of being dried using machinery, so as to greatly reduce production cost, improves enterprise's productivity effect；Not only improve animal and fowl fodder
The mixing of middle other components, simultaneously because corn pulp contains certain viscosity, after direct-injection mode puts into feed, corn pulp also rises
To the effect of binding agent, prevent feed nutrition material to scatter and disappear, effectively reduce the feed consumption rate during feeding, and feed life can be reduced
Dust during product, while so that feed is not pulverized easily in transportation；
(2) it is in pre-treatment step of the present invention that corn pulp phytase, bacillus amyloliquefaciens and aspergillus niger immixture is clear
Except the steps such as aspertoxin obtain can Direct-fed livestock and poultry corn pulp, while effectively reduce corn pulp in sulphite contain
Amount；And true albumen, Soluble phosphorus, total amino acid and TEAA content are improved in corn pulp, palatability is obviously improved, comprehensive
Nutritive value is significantly improved.Corn pulp after treatment is in the middle of feed so that corn pulp raw material availability is greatly improved；
In a word, processing method of the present invention is simple, and small investment can effectively improve corn pulp and be used in cultivation industry
Amount, solves the outlet problem of corn pulp, and alleviates China's feed protein situation that there is lack of raw materials, realizes zein resource profit
With maximization.
Specific embodiment
It is noted that described further below is all exemplary, it is intended to provide further instruction to the application.Unless another
Indicate, all technologies used herein and scientific terminology are with usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
Be also intended to include plural form, additionally, it should be understood that, when in this manual use term "comprising" or " including "
When, it indicates existing characteristics, step, operation, device, component or combinations thereof.
As background technology is introduced, unprocessed corn pulp water content is big, is unsuitable for being added directly in feed, adopts
Cut-off connects dry mode to be needed to consume mass energy, improves production cost.And due in corn pulp also containing aspertoxin,
Phytate, sulphite etc. is poisonous or ANFs, such as without treatment added to feed profit is not only greatly lowered in feed
With rate, and feeding livestock and poultry may be caused to be poisoned, be unfavorable for application of the corn pulp as feed ingredient.
In view of this, in a kind of typical implementation method of the invention, there is provided a kind of pretreated corn pulp, the jade
Rice ＆ peanut milk water content is 6-10%, and sulfite content is less than 1%, and viscosity is 50-80cps；
Compared with original corn pulp, the present invention is through true albumen, Soluble phosphorus, total amino acid in the corn pulp that obtains for the treatment of and always must
Amino acid content is needed to be improved, palatability is obviously improved, comprehensive nutrient value is significantly improved, while present invention gained corn pulp
Water content is significantly reduced compared with before processing so that corn pulp raw material availability is greatly improved.
In another typical implementation method of the invention, also disclose direct-injection of the corn pulp in animal and fowl fodder and use
Method, specifically, the direct-injection application method be by corn pulp direct spraying put into animal and fowl fodder in, the addition of the corn pulp
It is 3-6% to measure.
By in corn pulp direct spraying input feed, the mixing of other components in animal and fowl fodder is not only improved, while
Because corn pulp contains certain viscosity, after direct-injection mode puts into feed, corn pulp also acts as the effect of binding agent, prevents from raising
Material nutrient material scatters and disappears, while experiment proves that feed feed consumption rate can be significantly reduced, and can reduce feed preparation
Dust, while so that feed is not pulverized easily in transportation.
In another typical implementation method of the invention, the specific preprocess method of above-mentioned corn pulp is additionally provided, it is described
Preprocess method comprises the following steps：
(1) to adding alkali lye in corn pulp, stir process, adjusting corn slurry slurries pH to 5.2-5.3；
(2) to phytase is added in the corn pulp after regulation pH, treatment is stirred and evenly mixed；
(3) cultured bacillus amyloliquefaciens is respectively connected in the corn pulp after being digested to gained in step (2)
(Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) carry out fermented and cultured；Culture terminates
Afterwards, that is, corn pulp is obtained.
In another typical implementation method of the invention, the alkali lye is NaOH solution, and the concentration of NaOH solution is 1mol/
L；It is easy to adjust pH using NaOH solution, while the pH after regulation is conducive to phytase fully to volatilize enzymatic activity, so as to efficiently drop
Phytic acid in solution corn pulp.
In another typical implementation method of the invention, the phytase is liquid phytase, in terms of enzyme activity 5000U/ml,
Addition is 0.5~1.0L/m3Meter；35~40 DEG C of temperature during phytase, rotating speed is 25~35r/min, during stirring reaction
Between be 2~3h；Because the phytic acid in corn pulp and divalence or Tricationic, protein etc. are complexed, insoluble salt is formed, so that
Reduce its utilization rate, it is difficult to digested and absorbed by livestock and poultry, therefore it is to improve corn pulp utilization rate to eliminate phytic acid ANFs
Key.Because corn pulp now contains large quantity of moisture, phytase is fully mixed with corn pulp, in can effectively reducing corn pulp
Phytic acid, so as to be conducive to absorption of the corn pulp in livestock and poultry.
In another typical implementation method of the invention, the bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) and aspergillus niger (Aspergillus niger) inoculation order and the time be：First inoculation liquefaction is formed sediment
Afnyloliquefaciens, 100~150r/min of speed of agitator after 8~12h of aerobic fermentation, inoculates aspergillus niger, and speed of agitator 100~
150r/min, 24~48h of aerobic fermentation；Total inoculum concentration is 0.1~0.2% mass ratio, wherein bacillus amyloliquefaciens and black
Aspergillus mass ratio is 2~4：1, cultivation temperature is 30~35 DEG C, 32~60h of total fermentation time；Corn pulp is obtained；
In another typical implementation method of the invention, the bacillus amyloliquefaciens and aspergillus niger for using are the Ministry of Agriculture《Close
In microbiological feed bio-safety current techique criterion》Defined allows the production strain for using in annex, solves starch gemma bar
Bacterium bacterium numbering CICC 10074, aspergillus niger strain numbering is CICC 2106, is purchased from Chinese industrial Microbiological Culture Collection pipe
Reason center.Bacillus amyloliquefaciens and aspergillus niger can effectively remove AFB1 and zearalenone and its metabolin
α-zearalenol and ochratoxin A, while inventor has been surprisingly found that in experimental study, first inoculation solves starch gemma
After bacillus cultivates 24~48h, aspergillus niger is inoculated, the two shows strong cooperative effect, right in shorter fermentation time
Significantly improved in the clearance of toxin；Because original corn pulp is through and by determine, through the treatment of above-mentioned pre-treatment step
Afterwards, the soluble sulfite content in solution is minimized, and due to remaining in raw material corn pulp have a higher concentration can
Dissolubility sulphite (5~10%), although sulphite is widely used in food service industry as a kind of food additives, because
Its potential hazard and be strictly limited consumption, after above-mentioned pre-treatment step, corn pulp sulfite salt content
(content is less than 1%) is significantly reduced, and the sulphates content in corn pulp increases, therefore it is probably in microbial fermentation mistake to speculate
Oxidation is served to sulphite in journey, so as to effectively reduce the sulfite content in corn pulp.
And, after being processed using above-mentioned preprocess method, true albumen, Soluble phosphorus, total amino acid and total required ammonia in corn pulp
Base acid content is improved, and palatability is obviously improved, and comprehensive nutrient value is significantly improved, while present invention gained corn pulp is aqueous
Amount is significantly reduced compared with before processing so that corn pulp raw material availability is greatly improved.
It is further elaborated with reference to embodiment.
Embodiment 1
A kind of preprocess method of corn pulp, comprises the following steps：
(1) to 1mol/L NaOH solutions are added in corn pulp, stir process, adjusting corn starches slurries pH to 5.3；
(2) to liquid phytase is added in the corn pulp after regulation pH, in terms of enzyme activity 5000U/ml, temperature during phytase
35 DEG C of degree, rotating speed is 35r/min, and the stirring reaction time is 3h；Addition is 1.0L/m3Meter, stirs and evenly mixs treatment；
(3) cultured bacillus amyloliquefaciens are respectively connected in the corn pulp after being digested to gained in step (2)
(Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) carry out fermented and cultured；The Xie Dian
Afnyloliquefaciens (Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) inoculation order and
Time is：Bacillus amyloliquefaciens are first inoculated with, speed of agitator 150r/min after aerobic fermentation 8h, inoculates aspergillus niger, and stirring turns
Fast 150r/min, aerobic fermentation 24h；Total inoculum concentration is 0.2% mass ratio, wherein bacillus amyloliquefaciens and aspergillus niger mass ratio
It is 4：1, cultivation temperature is 35 DEG C, total fermentation time 32h；Corn pulp is obtained；
After being processed through the present embodiment pretreatment mode, phytic acid and its esters clearance are 96.7%, aspergillus flavus in corn pulp
Toxin B1 clearances 88.1%, zearalenone and its metabolin α-zearalenol clearance 68.6%, Aspergillus ochraceus poison
Plain A clearances 70.7%, sulfite content is 0.85%.
Embodiment 2
A kind of preprocess method of corn pulp, comprises the following steps：
(1) to 1mol/L NaOH solutions are added in corn pulp, stir process, adjusting corn starches slurries pH to 5.2；
(2) to liquid phytase is added in the corn pulp after regulation pH, in terms of enzyme activity 5000U/ml, temperature during phytase
40 DEG C of degree, rotating speed is 25r/min, and the stirring reaction time is 3h；Addition is 0.8L/m3Meter, stirs and evenly mixs treatment；
(3) cultured bacillus amyloliquefaciens are respectively connected in the corn pulp after being digested to gained in step (2)
(Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) carry out fermented and cultured；The Xie Dian
Afnyloliquefaciens (Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) inoculation order and
Time is：Bacillus amyloliquefaciens are first inoculated with, speed of agitator 100r/min after aerobic fermentation 12h, inoculates aspergillus niger, stirred
Rotating speed 100r/min, aerobic fermentation 48h；Total inoculum concentration is 0.1% mass ratio, wherein bacillus amyloliquefaciens and aspergillus niger quality
Than being 3：1, cultivation temperature is 30 DEG C, total fermentation time 60h；Corn pulp is obtained；
After being processed through the present embodiment pretreatment mode, phytic acid and its esters clearance are 98.2%, aspergillus flavus in corn pulp
Toxin B1 clearances 85.9%, zearalenone and its metabolin α-zearalenol clearance 65.9%, Aspergillus ochraceus poison
Plain A clearances 72.4%, sulfite content is 0.91%.
Embodiment 3
A kind of preprocess method of corn pulp, comprises the following steps：
(1) to 1mol/L NaOH solutions are added in corn pulp, stir process, adjusting corn starches slurries pH to 5.3；
(2) to liquid phytase is added in the corn pulp after regulation pH, in terms of enzyme activity 5000U/ml, temperature during phytase
37 DEG C of degree, rotating speed is 30r/min, and the stirring reaction time is 2h；Addition is 0.5L/m3Meter, stirs and evenly mixs treatment；
(3) cultured bacillus amyloliquefaciens are respectively connected in the corn pulp after being digested to gained in step (2)
(Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) carry out fermented and cultured；The Xie Dian
Afnyloliquefaciens (Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) inoculation order and
Time is：Bacillus amyloliquefaciens are first inoculated with, speed of agitator 150r/min after aerobic fermentation 8h, inoculates aspergillus niger, and stirring turns
Fast 150r/min, aerobic fermentation 24h；Total inoculum concentration is 0.2% mass ratio, wherein bacillus amyloliquefaciens and aspergillus niger mass ratio
It is 2：1, cultivation temperature is 35 DEG C, total fermentation time 32h；Corn pulp is obtained；
After being processed through the present embodiment pretreatment mode, phytic acid and its esters clearance are 95.2%, aspergillus flavus in corn pulp
Toxin B1 clearances 88.3%, zearalenone and its metabolin α-zearalenol clearance 61.3%, Aspergillus ochraceus poison
Plain A clearances 70.3%, sulfite content is 0.89%.
Comparative example 1
With embodiment 1, difference is by bacillus amyloliquefaciens (Bacillus to method
Amyloliquefaciens) and aspergillus niger (Aspergillus niger) while adding the corn pulp after enzymolysis in, total fermentation
Time-preserving is 32h.
After being processed through the present embodiment pretreatment mode, phytic acid and its esters clearance are 96.5%, aspergillus flavus in corn pulp
Toxin B1 clearances 51.9%, zearalenone and its metabolin α-zearalenol clearance 46.8%, Aspergillus ochraceus poison
Plain A clearances 45.6%, sulfite content is 0.87%, has significant difference with embodiment 1.
Experimental example
1. the selection of test material
Experiment is selected from the corn steep liquor product of Yishui branch company cornstarch Workshop Production, the corn steep liquor product with corn pulp
Prepared by embodiment 1 and the pretreatment mode of comparative example 1；
Experiment broiler chicken, meat duck are selected from raiser in certain Yishui region, and wherein broiler chicken, meat duck is young young from 0-5 week old,
Basal feed selects number mixed feed of commercially available broiler chicken and meat duck a number mixed feed (Xiamen Fu Shou Industrial Co., Ltd.).
2. test method and step
Broiler chicken, meat duck are divided into 3 groups at random respectively, every group each 150, the feed of wherein experimental group and control group is respectively
Number mixed feed of number mixed feed of broiler chicken and meat duck of the corn pulp of the present embodiment 1 and comparative example 1 of addition 4%, specifically
Addition manner is：By corn pulp, direct spraying input number mixed feed of broiler chicken and meat duck one coordinate feeding from small powder added holes
Material；Blank is only number mixed feed of number mixed feed of feeding dorking and meat duck.It is continuous to feed one month, broiler chicken, meat
Duck growing state is respectively as shown in table 1 below, 2：
The growth of meat chicken situation table of table 1
| Experimental group | Control group | Blank control group | |
| Average weight increasing a day/g | 58.6 | 52.2 | 50.5 |
| The incidence of disease/g | 0.2% | 1.4% | 0.3% |
| The death rate/g | 0 | 0 | 0 |
The meat duck growing state table of table 2
| Experimental group | Control group | Blank control group | |
| Average weight increasing a day/g | 45.8 | 41.2 | 38.7 |
| The incidence of disease/g | 0.2% | 1.1% | 0.4% |
| The death rate/g | 0 | 0 | 0 |
As can be seen from the table, after continuous feeding feed of the present invention, daily gain is compared with blank for experimental group broiler chicken, meat duck
Group is dramatically increased, while experimental group constitution is more healthy and strong, illness rate is lower in breeding process, death rate reduction, and control group
Although compared with blank control group, its Average weight increasing a day also be increased, and its incidence of disease is significantly improved compared with control group, show to make
Certain toxic action is produced to feeding animals with control group forage feed.Meanwhile, inventor has found, compared with blank control group,
The feed consumption rate of experimental group and control group is significantly reduced, and shows that corn pulp plays the effect of feed adhesive so as to reduce feed
Feed consumption rate, increases economic efficiency.
The preferred embodiment of the application is the foregoing is only, the application is not limited to, for the skill of this area
For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair
Change, equivalent, improvement etc., should be included within the protection domain of the application.
Claims (10)
1. a kind of pretreated corn pulp, it is characterised in that the corn pulp water content is 6-10%, sulfite content is small
In 1%, viscosity is 50-80cps.
2. a kind of corn pulp as claimed in claim 1 is in the direct-injection application method in animal and fowl fodder, it is characterised in that described
Method is that the addition of the corn pulp is 3-6% by corn pulp direct spraying input animal and fowl fodder.
3. a kind of preprocess method of corn pulp as claimed in claim 1, it is characterised in that comprise the following steps：
(1) to adding alkali lye in corn pulp, stir process, adjusting corn slurry slurries pH to 5.2-5.3；
(2) to phytase is added in the corn pulp after regulation pH, treatment is stirred and evenly mixed；
(3) cultured bacillus amyloliquefaciens (Bacillus is respectively connected in the corn pulp after being digested to gained in step (2)
) and aspergillus niger (Aspergillus niger) carries out fermented and cultured amyloliquefaciens；After culture terminates, that is, obtain jade
Rice ＆ peanut milk material.
4. a kind of preprocess method of corn pulp as claimed in claim 3, it is characterised in that the alkali lye is NaOH solution,
The concentration of NaOH solution is 1mol/L.
5. a kind of preprocess method of corn pulp as claimed in claim 3, it is characterised in that the phytase is liquid phytic acid
Enzyme, in terms of enzyme activity 5000U/ml, addition is 0.5~1.0L/m3。
6. a kind of preprocess method of corn pulp as claimed in claim 3, it is characterised in that the phytase temperature is
35~40 DEG C, rotating speed is 25~35r/min, and the stirring reaction time is 2~3h.
7. a kind of preprocess method of corn pulp as claimed in claim 3, it is characterised in that the bacillus amyloliquefaciens
(Bacillus amyloliquefaciens) and aspergillus niger (Aspergillus niger) inoculation order be：First inoculation is solved
Bacillus amyloliquefacienses, fermented and cultured for a period of time after, inoculate aspergillus niger.
8. a kind of preprocess method of corn pulp as claimed in claim 7, it is characterised in that the specific inoculation condition is：
Bacillus amyloliquefaciens are first inoculated with, 100~150r/min of speed of agitator after 8~12h of aerobic fermentation, inoculates aspergillus niger, stirred
100~150r/min of rotating speed, 24~48h of aerobic fermentation；Total inoculum concentration is 0.1~0.2% mass ratio.
9. a kind of preprocess method of the corn pulp as described in claim 3 or 8, it is characterised in that the solution starch gemma bar
Bacterium and aspergillus niger mass ratio are 2~4：1, cultivation temperature is 30~35 DEG C, 32~60h of total fermentation time.
10. a kind of preprocess method of corn pulp as claimed in claim 3, it is characterised in that the bacillus amyloliquefaciens
Bacterium numbering CICC 10074, aspergillus niger strain numbering is CICC 2106, is purchased from the management of Chinese industrial Microbiological Culture Collection
Center.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710210039.XA CN106858042B (en) | 2017-03-31 | 2017-03-31 | Pretreated corn steep liquor and direct injection use method thereof in livestock and poultry feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710210039.XA CN106858042B (en) | 2017-03-31 | 2017-03-31 | Pretreated corn steep liquor and direct injection use method thereof in livestock and poultry feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106858042A true CN106858042A (en) | 2017-06-20 |
| CN106858042B CN106858042B (en) | 2020-03-13 |
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| CN109077182A (en) * | 2018-08-28 | 2018-12-25 | 山东福之宽生物饲料有限公司 | Corn pulp Non-toxic treatment method |
| CN109852568A (en) * | 2019-03-21 | 2019-06-07 | 伊犁川宁生物技术有限公司 | A kind of improved corn steep liquor and its preparation method and application of abomacetin fermentation production |
| CN111606756A (en) * | 2020-06-29 | 2020-09-01 | 黄河三角洲京博化工研究院有限公司 | Method for producing liquid fertilizer by using corn steep liquor |
| CN111733114A (en) * | 2020-08-04 | 2020-10-02 | 山东理工大学 | Method for preparing microecological bactericide by using corn steep liquor as raw material and utilizing symbiotic fermentation technology |
| WO2023017710A1 (en) * | 2021-08-13 | 2023-02-16 | 株式会社フジワラテクノアート | Filamentous fungi solid culture, composition, food, animal feedstuff, food additive, or animal feedstuff additive, and method for producing filamentous fungi solid culture |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109077182A (en) * | 2018-08-28 | 2018-12-25 | 山东福之宽生物饲料有限公司 | Corn pulp Non-toxic treatment method |
| CN109852568A (en) * | 2019-03-21 | 2019-06-07 | 伊犁川宁生物技术有限公司 | A kind of improved corn steep liquor and its preparation method and application of abomacetin fermentation production |
| CN111606756A (en) * | 2020-06-29 | 2020-09-01 | 黄河三角洲京博化工研究院有限公司 | Method for producing liquid fertilizer by using corn steep liquor |
| CN111733114A (en) * | 2020-08-04 | 2020-10-02 | 山东理工大学 | Method for preparing microecological bactericide by using corn steep liquor as raw material and utilizing symbiotic fermentation technology |
| WO2023017710A1 (en) * | 2021-08-13 | 2023-02-16 | 株式会社フジワラテクノアート | Filamentous fungi solid culture, composition, food, animal feedstuff, food additive, or animal feedstuff additive, and method for producing filamentous fungi solid culture |
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| CN106858042B (en) | 2020-03-13 |
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