CN106857665A - A kind of bacteriostatic agent, its preparation method and application - Google Patents
A kind of bacteriostatic agent, its preparation method and application Download PDFInfo
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- CN106857665A CN106857665A CN201710077720.1A CN201710077720A CN106857665A CN 106857665 A CN106857665 A CN 106857665A CN 201710077720 A CN201710077720 A CN 201710077720A CN 106857665 A CN106857665 A CN 106857665A
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- bacteriostatic agent
- bamboo vinegar
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to field of biological, a kind of bacteriostatic agent, its preparation method and application are related generally to, and in particular to a kind of bacteriostatic agent for preventing and treating bacterial brown spot, its preparation method and application, the bacteriostatic agent includes bamboo vinegar and shitosan；Wherein, the volume ratio of the bamboo vinegar and shitosan is (1 20):1.Bacteriostatic agent of the present invention is with strong points, by specific bamboo vinegar and the ratio of shitosan, interacts, and specifically for Trust's Pseudomonas alba of mushroom can effectively kill Trust's Pseudomonas alba, prevents and treats the brown spot of mushroom.
Description
Technical field
The present invention relates to field of biological, a kind of bacteriostatic agent, its preparation method and application are related generally to, and in particular to one
Plant bacteriostatic agent, its preparation method and the application for preventing and treating bacterial brown spot.
Background technology
Flat mushroom is one of big edible mushroom in the world four, is also the maximum edible mushroom of cultivation amount.It is annual because pest and disease damage and caused by
Economic loss is difficult to estimate.In recent years, by putting down that Trust Pseudomonas alba Pseudomonas tolaasii Paine cause
Mushroom bacterial brown spot harm weight, and host range is wide, can also infect agaricus bisporus Agaricus bisporus, asparagus
The multiclass edible mushroom such as Flammulina velutipes.With the implementation and the gradually expansion of cultivation scale of whole year production, flat mushroom
Bacterial brown spot is increasingly serious, especially season in spring and autumn.Once there is brown spot in mushroom, thoroughly preventing and treating is difficult to, often
Aggravate year by year, the incidence of disease is up to more than 80% when serious.Bacterial brown spot not only influences Yield of Pleurotus Ostreatus, it is often more important that reduce flat
Mushroom quality, causes serious loss, in the urgent need to seeking effective prevention and control means to flat mushroom production every year.
The major measure that control flat mushroom bacterial brown spot spreads at present is cultural control and sprays antibiotic, but antibiosis
The various problems for widely using and causing of element are also comed one after another, and such as cause residual hazard, soil property to degenerate, pollute environment and drug-fast
Produce etc..Very big threat is caused for health, ecological environment, is unfavorable for the sustainable development of agricultural production.At present
Green agriculture, food security, pollution-free vegetable, the concept of pollution-free vegetable are increasingly rooted in the hearts of the people.
Since 1980, the biological control method of plant pest has been carried out gradually by the weight of people using biological agent
Depending on.Due to bamboo vinegar complicated component in itself, main component is organic acid, phenols, ketone, alcohols and esters etc., these compositions tool
Having has synergistic function between biocidal property, and heterogeneity, be the bacteriostatic agent of natural, nontoxic safety.
The A of CN 1537630 disclose a kind of containing bamboo vinegar, shitosan, the medicament of silver ion and its preparation method and application, should
Medicament is used to prevent and treat the disease of skin such as tinea pedis.This application do not disclose the ratio of specific bamboo vinegar and shitosan, and it is directed to
It is disease of skin.At present, bamboo vinegar is there is no in the world as the report of the biological pesticide of flat mushroom bacterial brown spot.
The content of the invention
Effective biological control means are lacked to flat mushroom bacterial brown spot at present it is an object of the invention to be directed to, there is provided
A kind of bacteriostatic agent, its preparation method and application, the bacteriostatic agent can effectively prevent and treat flat mushroom bacterial brown spot, it is possible to significantly
Improve Yield of Pleurotus Ostreatus.
To achieve the above object, the present invention is adopted the following technical scheme that：
On the one hand, the present invention provides a kind of bacteriostatic agent, and the bacteriostatic agent includes bamboo vinegar and shitosan；
Wherein, the volume ratio of the bamboo vinegar and shitosan is (1-20):1.
The volume ratio of the bamboo vinegar and shitosan is 1:1、2:1、3:1、4:1、5:1、6:1、7:1、8:1、9:1、10:1、
11:1、12:1、13:1、14:1、15:1、16:1、17:1、18:1、19:1 or 20:1.
In the present invention, some mushrooms can produce brown spot in plantation, because Trust's Pseudomonas alba causes, meeting
Propagate mutually, cause the death of mushroom, and bacteriostatic agent of the present invention is by specific bamboo vinegar and the ratio of shitosan, phase interaction
With, Trust's Pseudomonas alba can effectively specifically for Trust's Pseudomonas alba of mushroom be killed, prevent and treat bacterium
The brown spot of class.
Preferably, the volume ratio of the bamboo vinegar and shitosan is (4-16):1, preferably (8-12):1, further preferably
It is 10:1.
Preferably, the mass percent of the bamboo vinegar is 1-10%, for example can be 1%, 2%, 3%, 4%, 5%,
6%th, 7%, 8%, 9% or 10%, preferably 2-6%, more preferably 4%.
Preferably, the mass percent of the shitosan is 0.1-5%, for example can be 0.1%, 0.2%, 0.3%,
0.4%th, 0.5%, 0.6%, 0.7%, 0.9%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%,
3%th, 3.2%, 3.5%, 3.8%, 4%, 4.2%, 4.5%, 4.8% or 5%, preferably 0.3-3%, more preferably
0.5%.
Second aspect, the present invention provides the preparation method of bacteriostatic agent as described in relation to the first aspect, comprises the following steps：
(1) bamboo vinegar and activated carbon powder are mixed, stirring stands, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar that step (1) is obtained is mixed by formula ratio with shitosan, is stirred, be prepared into
To the bacteriostatic agent.
Preferably, the mass volume ratio of the activated carbon and bamboo vinegar is 1:(10-40), for example, can be 1:10、1:11、
1:12、1:13、1:14、1:15、1:16、1:17、1:18、1:19、1:20、1:21、1:22、1:23、1:24、1:25、1:26、1:
27、1:28、1:29、1:30、1:31、1:32、1:33、1:34、1:35、1:36、1:37、1:38、1:39 or 1:40, preferably 1:
(12-30), more preferably 1:(15-20), most preferably 1:20.
Preferably, the mixing time described in step (1) be 0.2-5h, for example can be 0.2h, 0.3h, 0.4h, 0.5h,
0.6h、0.7h、0.8h、0.9h、1h、1.2h、1.4h、1.5h、1.8h、1.9h、2h、2.2h、2.3h、2.5h、2.8h、3h、
3.2h, 3.3h, 3.5h, 3.8h, 4h, 4.2h, 4.5h, 4.8h or 5h, preferably 0.3-3h, more preferably 0.5h.
Preferably, the time of repose described in step (1) is 1-10 days, for example can be 1 day, 2 days, 3 days, 4 days, 5 days, 6
My god, 7 days, 8 days, 9 days or 10 days, preferably 2-8 days, most preferably more preferably 3-6 days, 3 days.
Preferably, the mixing time described in step (2) be 1-20min, for example can be 1min, 2min, 3min, 4min,
5min、6min、7min、8min、9min、10min、11min、12min、13min、14min、15min、16min、17min、
18min, 19min or 20min, preferably 2-15min, more preferably 3-10min, most preferably 3min.
Used as optimal technical scheme, the preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:Activated carbon powder and the bamboo vinegar mixing of (10-40), stir 0.2-5h, stand 1-10
My god, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume (1-20):1 mixes with shitosan, stirs 1-
20min, is well mixed, and prepares the bacteriostatic agent.
The third aspect, the present invention provides a kind of antibacterial cultivation solution, and the antibacterial cultivation solution includes the suppression described in first aspect
Microbial inoculum.
Fourth aspect, the present invention provides a kind of growth promoter, including the bacteriostatic agent that such as first aspect is stated.
Preferably, the mass concentration of the bacteriostatic agent is 1-5%, for example can be 1%, 1.5%, 2%, 2.5%, 3%,
3.5%th, 4%, 4.5% or 5%, preferably 1-3%, more preferably 2.5%.
The concentration of bacteriostatic agent of the present invention is mixed in compost in more than 1-5%, plays the effect for promoting mycelial growth.
5th aspect, the present invention provides a kind of bacteriostatic agent as described in relation to the first aspect, the preparation side as described in second aspect
Bacteriostatic agent, the antibacterial cultivation solution as described in the third aspect or the agricultural chemicals as described in fourth aspect that method is prepared be used for flat mushroom,
In asparagus or agaricus bisporus any one or at least two combination bacterial brown spot preventing and treating.
Compared with prior art, the invention has the advantages that：
(1) bacteriostatic agent of the present invention is with strong points, by specific bamboo vinegar and the ratio of shitosan, interacts, can
Specific Trust's Pseudomonas alba for mushroom, can effectively kill Trust's Pseudomonas alba, prevent and treat the brown of mushroom
Pinta；
(2) the antibacterial band of bacteriostatic agent prepared by the present invention can reach more than 8.95mm, prevention effect up to 73.21% with
On；
(3) bacteriostatic agent preparation process of the present invention is simple, it is easy to implements and operates, and is capable of achieving industrialization large-scale production, can
For enterprise brings good economic benefit, possesses wide prospect.
Specific embodiment
Further to illustrate technological means and its effect that the present invention is taken, it is preferable to carry out below in conjunction with of the invention
Example further illustrates technical scheme, but the present invention is not limited in scope of embodiments.
Experimental technique used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc. in following embodiments, unless otherwise specified, commercially obtain.
Embodiment 1
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:20 activated carbon powder and mass percent mixes for 4% bamboo vinegar, stirs 0.5h,
3 days are stood, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 10:1 with mass percent be 0.5% shell gather
Sugar mixing, stirs 3min, is well mixed, and prepares the bacteriostatic agent.
Embodiment 2
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:10 activated carbon powder and mass percent mixes for 5% bamboo vinegar, stirs 0.2h,
1 day is stood, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 1:1 is 1% shitosan with mass percent
Mixing, stirs 1min, is well mixed, and prepares the bacteriostatic agent.
Embodiment 3
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:15 activated carbon powder and mass percent mixes for 2% bamboo vinegar, stirs 0.8h,
2 days are stood, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 3:1 with mass percent be 0.3% shell gather
Sugar mixing, stirs 5min, is well mixed, and prepares the bacteriostatic agent.
Embodiment 4
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:25 activated carbon powder and mass percent mixes for 6% bamboo vinegar, stirs 1h, quiet
Put 5 days, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 8:1 is 3% shitosan with mass percent
Mixing, stirs 8min, is well mixed, and prepares the bacteriostatic agent.
Embodiment 5
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:30 activated carbon powder and mass percent mixes for 1% bamboo vinegar, stirs 2h, quiet
Put 4 days, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 12:1 with mass percent be 0.1% shell gather
Sugar mixing, stirs 10min, is well mixed, and prepares the bacteriostatic agent.
Embodiment 6
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:32 activated carbon powder and mass percent mixes for 10% bamboo vinegar, stirs 3h, quiet
Put 6 days, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 15:1 is 5% shitosan with mass percent
Mixing, stirs 12min, is well mixed, and prepares the bacteriostatic agent.
Embodiment 7
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:35 activated carbon powder and bamboo vinegar mixing, stirs 4h, stands 7 days, takes out supernatant
That is bamboo vinegar；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 18:1 mixes with shitosan, stirs 15min,
It is well mixed, prepare the bacteriostatic agent.
Embodiment 8
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:40 activated carbon powder and bamboo vinegar mixing, stirs 5h, stands 10 days, takes out supernatant
Liquid is bamboo vinegar；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 20:1 mixes with shitosan, stirs 20min,
It is well mixed, prepare the bacteriostatic agent.
Embodiment 9
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:38 activated carbon powder and bamboo vinegar mixing, stirs 3.5h, stands 8 days, takes out supernatant
Liquid is bamboo vinegar；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 13:1 mixes with shitosan, stirs 12min,
It is well mixed, prepare the bacteriostatic agent.
Embodiment 10
The preparation method of the bacteriostatic agent comprises the following steps：
(1) it is 1 by mass volume ratio:16 activated carbon powder and bamboo vinegar mixing, stirs 2.5h, stands 3 days, takes out supernatant
Liquid is bamboo vinegar；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume 10:1 mixes with shitosan, stirs 5min,
It is well mixed, prepare the bacteriostatic agent.
Comparative example 1
Comparative example 1 is different only in that bamboo vinegar is by volume 0.5 with embodiment 1:1 mixes with shitosan, other groups
The ratio and preparation method divided are all consistent with embodiment 1.
Comparative example 2
Comparative example 2 is different only in that bamboo vinegar is by volume 25 with embodiment 1:1 mixes with shitosan, other components
Ratio and preparation method it is all consistent with embodiment 1.
Comparative example 3
CN 1537630 A
Comparative example 3 also added silver ion with being different only in that for embodiment 1, and the ratio and preparation method of other components are all
It is consistent with embodiment 1.
Comparative example 4
CN 103931741 A
Comparative example 4 also added 2 parts of Tea Polyphenols, 2 parts of green tea powder, pepper extract 8 with being different only in that for embodiment 1
Part, 10 parts of Chinese prickly ash leaf extract, the ratio and preparation method of other components are all consistent with embodiment 1.
Bacteriostatic agent prepared by embodiment 1-10 and comparative example 1-4 is carried out into bacteriostasis measure and field test, as a result such as
Shown in table 1 below；
Table 1
As it can be seen from table 1 the antibacterial band of the bacteriostatic agent of the application preparation can reach more than 8.95mm, prevention effect is reachable
More than 73.21%, and pass through the contrast of embodiment and comparative example it can be found that the ratio regular meeting of regulation bamboo vinegar and shitosan is obvious
The fungistatic effect of bacteriostatic agent is reduced, and adds other component also to reduce the fungistatic effect of bacteriostatic agent.
Embodiment 11
Bacteriostatic agent prepared by embodiment 1 is diluted to 2.5% concentration, is added into dispensing, stirred, planted
Plant, promote the growth of mycelia.
Embodiment 12
Bacteriostatic agent prepared by embodiment 1 is diluted to 3% concentration, is added into dispensing, stirred, planted,
Promote the growth of mycelia.
Embodiment 13
Bacteriostatic agent prepared by embodiment 1 is diluted to 5% concentration, is added into dispensing, stirred, planted,
Promote the growth of mycelia.
Applicant states that the present invention illustrates method detailed of the invention by above-described embodiment, but the present invention not office
It is limited to above-mentioned method detailed, that is, does not mean that the present invention has to rely on above-mentioned method detailed and could implement.Art
Technical staff it will be clearly understood that any improvement in the present invention, equivalence replacement and auxiliary element to each raw material of product of the present invention
Addition, selection of concrete mode etc., within the scope of all falling within protection scope of the present invention and disclosing.
Claims (10)
1. a kind of bacteriostatic agent, it is characterised in that the bacteriostatic agent includes bamboo vinegar and shitosan；
Wherein, the volume ratio of the bamboo vinegar and shitosan is (1-20):1.
2. bacteriostatic agent according to claim 1, it is characterised in that the volume ratio of the bamboo vinegar and shitosan is (4-
16):1, preferably (8-12):1, more preferably 10:1.
3. bacteriostatic agent according to claim 1 and 2, it is characterised in that the mass percent of the bamboo vinegar is 1-10%,
Preferably 2-6%, more preferably 4%；
Preferably, the mass percent of the shitosan is 0.1-5%, preferably 0.3-3%, more preferably 0.5%.
4. the preparation method of a kind of bacteriostatic agent as any one of claim 1-3, it is characterised in that including following step
Suddenly：
(1) bamboo vinegar and activated carbon powder are mixed, stirring stands, it is bamboo vinegar to take out supernatant；
(2) the supernatant i.e. bamboo vinegar that step (1) is obtained is mixed by formula ratio with shitosan, is stirred, prepare institute
State bacteriostatic agent.
5. preparation method according to claim 4, it is characterised in that the mass volume ratio of the activated carbon and bamboo vinegar is
1:(10-40), preferably 1:(12-30), more preferably 1:(15-20), most preferably 1:20.
6. the preparation method according to claim 4 or 5, it is characterised in that the mixing time described in step (1) is 0.2-
5h, preferably 0.3-3h, more preferably 0.5h；
Preferably, the time of repose described in step (1) be 1-10 days, preferably 2-8 days, more preferably 3-6 days, most preferably
It is 3 days；
Preferably, the mixing time described in step (2) be 1-20min, more preferably preferably 2-15min, 3-10min,
Most preferably 3min.
7. the preparation method according to any one of claim 4-6, it is characterised in that comprise the following steps：
(1) it is 1 by mass volume ratio:Activated carbon powder and the bamboo vinegar mixing of (10-40), stir 0.2-5h, stand 1-10 days, take
Go out supernatant i.e. bamboo vinegar；
(2) the supernatant i.e. bamboo vinegar for obtaining step (1) is by volume (1-20):1 mixes with shitosan, stirs 1-
20min, is well mixed, and prepares the bacteriostatic agent.
8. a kind of antibacterial cultivation solution, it is characterised in that the antibacterial cultivation solution includes the suppression any one of claim 1-3
Microbial inoculum.
9. a kind of growth promoter, it is characterised in that including the bacteriostatic agent any one of requiring 1-3 such as profit；
Preferably, the mass concentration of the bacteriostatic agent is 1-5%, preferably 1-3%, more preferably 2.5%.
10. a kind of bacteriostatic agent as any one of profit requires 1-3, the preparation method as described in claim 4-7 are prepared into
Bacteriostatic agent, antibacterial cultivation solution as claimed in claim 8 or the agricultural chemicals as claimed in claim 9 for arriving are used for flat mushroom, asparagus
Or the preventing and treating of the bacterial brown spot of any one in agaricus bisporus.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102503667A (en) * | 2011-10-21 | 2012-06-20 | 北京雷力农用化学有限公司 | Foliage fertilizer and preparation method thereof |
| CN102631006A (en) * | 2012-03-26 | 2012-08-15 | 孙涛 | Natural compound food antiseptic preservative |
-
2017
- 2017-02-13 CN CN201710077720.1A patent/CN106857665B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102503667A (en) * | 2011-10-21 | 2012-06-20 | 北京雷力农用化学有限公司 | Foliage fertilizer and preparation method thereof |
| CN102631006A (en) * | 2012-03-26 | 2012-08-15 | 孙涛 | Natural compound food antiseptic preservative |
Non-Patent Citations (4)
| Title |
|---|
| 刘海英等: ""壳聚糖对平菇褐斑病菌的抑制作用"", 《食用菌》 * |
| 廖晓珊等: ""壳聚糖协同竹醋液的抗菌性研究"", 《中国食品添加剂》 * |
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