CN106822377A - A kind of compound osteitis bar cloth patch and preparation method thereof - Google Patents
A kind of compound osteitis bar cloth patch and preparation method thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
- A61K9/703—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms characterised by shape or structure; Details concerning release liner or backing; Refillable patches; User-activated patches
- A61K9/7038—Transdermal patches of the drug-in-adhesive type, i.e. comprising drug in the skin-adhesive layer
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The invention provides a kind of compound osteitis bar cloth patch and preparation method thereof.Bar cloth patch is on the basis of the stifling proved recipe of seedling doctor is fully excavated, it is prepared from by the rhizome of davallia, jatamans valeriana rhizome, the bark of eucommia, scythian lamb rhizome, toddalia, reticulate millettia, yellow angledtwig magnoliavine root stem and leaf, Heiguteng exract, HERBA EPIMEDII, deep perfume, lycopodium calvatum, the taste medicinal material of sargentodoxa cuneata 12, Chinese medicine preparation is of a tightly knit structure, preparation technology is suitably simple, overcome shortcoming of the seedling doctor's fumigating system in clinical application, its drugloading rate is big, drug availability is high, action is rapid, for treating Osteoarthritis effect highly significant, clinical application is especially suitable for.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, and in particular to a kind of compound osteitis bar cloth patch and preparation method thereof.
Background technology
Osteoarthritis is one of clinically common illness, and the later stage can cause head necrosis, the dysfunction of hip joint,
Have a strong impact on the quality of life and labour capacity of patient.The Patients with Aseptic Necrosis of Femoral of China between 5,000,000-800 ten thousand people, every year
New cases more than 150,000 people.Clinically medical expense is high for the disease, the cycle is long, and curative effect is also not satisfied, to patient and
Society brings greatly burden.
Seedling medicine fumigation and steaming therapy is a kind of representative external therapy of Miao ethnic group's medical science, and seedling doctor's fumigation and steaming therapy is the water evaporated with medicine
A kind of method of the stifling patient's lesion site treatment disease of gas, efficacy component can enter with medicine vapour through respiratory tract and the pore of expansion
In vivo.Seedling doctor is kind, and fresh medicine is treated, seedling is cured fumigation and steaming therapy and combines medication and thermotherapy, and thermotherapy can make pars affecta skin, muscle
Telangiectasis, accelerate blood circulation, with composite medicine can make vivotoxin accelerate excretion, enhance metabolism, make skin,
The obstacles such as ligament, tendon, muscles and bones, blood vessels are released, and human body is recovered normal function, so as to reach the purpose for the treatment of.
But seedling doctor's fumigation and steaming therapy there is also problems with Clinical practice：Apparatus operation inconvenience in itself, patient skin
Directly with the easy cross-infection of stifling instrument contacts, and the selection of when and where is inconvenient, and all treatments to patient bring difficulty,
It is difficult to popularization and application.Exception, the stifling proved recipe of seedling doctor is mainly experience prescription among the people, passed on from one to another with experience with medicine composition dosage based on,
Lack scientific and reasonable optimization, its clinical application is main based on auxiliary treatment, fail preferably to play the value of seedling medicine.
Ba Bu patches are a kind of external patch paste, have more long applicating history in Japan.Be medicinal substances extract, medicinal material or/and
After chemicals mixes with suitable hydrophilic matrix, the emplastrum being made on back lining materials is coated on, by backing (conventional nothing
Spin cloth, stretch fabric), lotion, anti-mucous membrane (barrier film on lotion surface) composition.But because its selection to medicine is (in especially
Medicine medicinal extract) and the selection requirement of matrix it is higher, be difficult screening, the factor such as research cost is high directly governs the patch development of bar cloth, closely
Nian Lai, as domestic and international medicament is academic and its application is developed rapidly, medication simplicity that transdermal delivery system institute uniqueness has
One of most noticeable field in 21 century drug development is made it with security.
Therefore, the present inventor is the deficiency of solution fumigation and steaming therapy, and its curative effect is played to greatest extent, excavates folk remedy, with
Phase a kind of effective, treatment method conveniently, safely and medicine for Osteoarthritis are provided, it is smoked that development had both retained traditional seedling doctor
Effect and economic convenient bar cloth patch of therapy are steamed, the potential value of seedling medicine is given full play to, will be had to the medical development of seedling
Significance.
The content of the invention
In order to solve the above technical problems, the invention provides a kind of compound osteitis bar cloth patch and preparation method thereof.The bar cloth
Patch is prepared from the basis of the stifling proved recipe of seedling doctor is fully excavated, and its formulation composition is rigorous, preparation technology is suitably simple,
Shortcoming of the seedling doctor's fumigating system in clinical application is overcome, its drugloading rate is big, drug availability is high, action is rapid, for treating
Osteoarthritis effect highly significant.
Specifically, the technical scheme is that：
A kind of compound osteitis bar cloth patch, is mainly made up of following matrix and Chinese medical extract：
Matrix：By weight, by Polyvinylcaprolactame-polyvinyl acetate-polyethyleneglycol-graft copolymer
(soluplus) 7-9 parts, gelatin 7-9 parts, Sodium Polyacrylate 5-7 parts, titanium dioxide 7-9 parts, sodium carboxymethylcellulose 5-7 parts,
Polyvinylpyrrolidone 20-40 parts, tartaric acid 1-2 parts, Dihydroxyaluminium Aminoacetate 0.1-1 parts, glycerine 140-180 parts, distilled water 50-90 parts of tune
Conjunction is prepared from
Chinese medical extract：By rhizome of davallia 5-10 parts, jatamans valeriana rhizome 5-10 parts, bark of eucommia 5-10 parts, scythian lamb rhizome 5-10 parts, flying dragon
The palm 5-10 parts of blood, reticulate millettia 5-10 parts, yellow angledtwig magnoliavine root stem and leaf 5-10 parts, Heiguteng exract 5-10 parts, HERBA EPIMEDII 5-10 parts, it is deep it is fragrant 5-10 parts,
Lycopodium calvatum 5-10 parts, sargentodoxa cuneata 5-10 parts extracted forms.
Further, a kind of compound osteitis bar cloth patch, is mainly made up of following matrix and Chinese medical extract：
Matrix：By weight, by Polyvinylcaprolactame-polyvinyl acetate-polyethyleneglycol-graft copolymer
(soluplus) 8 parts, 8 parts of gelatin, 6 parts of Sodium Polyacrylate, 8 parts of titanium dioxide, 6 parts of sodium carboxymethylcellulose, polyvinyl pyrrole
30 parts of alkanone, 1.5 parts of tartaric acid, 0.5 part of Dihydroxyaluminium Aminoacetate, 160 parts of glycerine, 70 parts of blendings of distilled water are prepared from；
Chinese medical extract：By weight, by 8 parts of the rhizome of davallia, 8 parts of jatamans valeriana rhizome, 8 parts of the bark of eucommia, 8 parts of scythian lamb rhizome, flying dragon
The palm 8 parts of blood, 8 parts of reticulate millettia, 8 parts of yellow angledtwig magnoliavine root stem and leaf, 8 parts of Heiguteng exract, 8 parts of HERBA EPIMEDII, deep fragrant 8 parts, 8 parts of lycopodium calvatum, 8 parts of sargentodoxa cuneata
It is extracted to form.
Further, the ratio of the compound osteitis bar cloth patch mesostroma and Chinese medical extract is 10:1-5；Preferred substrate
It is 10 with the ratio of Chinese medical extract:3.
It is prepared from by following steps according to Chinese medical extract described above：
Weigh the rhizome of davallia, jatamans valeriana rhizome, the bark of eucommia, scythian lamb rhizome, toddalia, reticulate millettia, yellow angledtwig magnoliavine root stem and leaf, Heiguteng exract, HERBA EPIMEDII,
Deep perfume, lycopodium calvatum, the taste medicinal material of sargentodoxa cuneata 12, plus the decocting of 8 times of amounts are boiled 2 times, and 1.5 hours every time, filtrate was reclaimed in filtering, dense
Contracting, dry, pulverize 200 mesh sieves, obtain final product Chinese medical extract.
Present invention also offers a kind of preparation method of compound osteitis bar cloth patch, comprise the following steps：
Accurately weighed Sodium Polyacrylate, titanium dioxide is mixed uniformly, adds glycerine, and grinding is uniform, used as A phases.
Accurately weighed sodium carboxymethylcellulose, gelatin, polyvinylpyrrolidone, Polyvinylcaprolactame-polyvinyl acetate-poly- second again
Glycol graft copolymer (soluplus) is mixed uniformly, is adding water, and oil bath is added makes it fully swelling, used as B phases.Will
A, B two-phase are ground, and add tartaric acid and Dihydroxyaluminium Aminoacetate, add Chinese medical extract, are well mixed.Coated backing
On, dry and obtain bar cloth patch.
Beneficial effects of the present invention are：
First, during to the pharmacodynamic study of traditional " seedling doctor fumigation and steaming therapy " and classical " the stifling proved recipe of seedling doctor ", find
Its big multi-party medicine is what experience was mutually handed down, and its formula dosage is also more optionally combined, and lacks scientific validation, causes clinical medicine
Effect is not enough significantly, clinical main at present to cure fumigation and steaming therapy as based on auxiliary treatment using seedling the problems such as drug effect works slow.It is this
Invention has carried out the collocation again of primary and secondary medication, final to determine that prescription is by multiple recipe determination：The rhizome of davallia, jatamans valeriana rhizome,
The bark of eucommia, scythian lamb rhizome, toddalia, reticulate millettia, yellow angledtwig magnoliavine root stem and leaf, Heiguteng exract, HERBA EPIMEDII, deep perfume, lycopodium calvatum, the taste of sargentodoxa cuneata 12
Medicinal material is constituted.The rhizome of davallia：Nature and flavor：Hardship, temperature.Return kidney, Liver Channel, with The strong bone of kidney tonifying, the effect of continues and hinders pain relieving.Jatamans valeriana rhizome：Taste is pungent；
Slight bitter；It is warm in nature, qi-regulating and in, dispelling cold and removing dampness；Activating blood circulation and reducing swelling.The bark of eucommia：Its is sweet, warm in nature.Tonify the liver and kidney, strengthen muscles and bones, adjust
Reason punching is appointed, effect of strengthen meridian and miscarriage prevention.Scythian lamb rhizome：It is bitter, sweet, temperature.Filling liver kidney, strong spinal column, wind-damp dispelling.Toddalia：Wind-dispelling stops
Bitterly, dissipate stasis of blood hemostasis；Reticulate millettia：Meridian distribution of property and flavor:It is bitter, sweet, temperature.Return liver and kidney channel.Cure mainly:Enrich blood, invigorate blood circulation, dredging collateral.Yellow angledtwig magnoliavine root stem and leaf：It is main
Promoting the circulation of qi is controlled, is relieved pain, promoting blood circulation dissipates the stasis of blood；Heiguteng exract：Invigorate blood circulation, wind-damp dispelling.HERBA EPIMEDII：Also known as:Barrenwort, kidney-replenishing, strong muscle
Bone, wind-damp dispelling.Deep perfume (or spice)：Dispelling wind and eliminating dampness, it is promoting blood circulation and removing obstruction in channels.Lycopodium calvatum：Expelling wind and clearing away cold dehumidification swellings, stimulate the circulation of the blood and cause the muscles and joints to relax.Sargentodoxa cuneata：
Toxin-Vanquishing disappears carbuncle, promoting blood circulation and removing obstruction in channels, the effect of wind dispelling insecticide；The party is conceived using the prescriptions that make of the assistant of three monarchs, three minister three three, with the rhizome of davallia,
The bark of eucommia, HERBA EPIMEDII, using Heiguteng exract, lycopodium calvatum, reticulate millettia as ministerial drug, are total to principal drug assistance medicine prestige, with scythian lamb rhizome, spider as monarch
Fragrant, yellow angledtwig magnoliavine root stem and leaf is adjutant, and medicine is made with toddalia, deep fragrant, sargentodoxa cuneata, all medicines is floated, and 12 taste medicines complement each other, and assist
Synergy is adjusted, drug effect is gathered altogether, its property of medicine is big, be particularly suitable for being made bar cloth patch.The present invention is fitted to bar cloth patch matrix and Chinese medical extract
Answering property has carried out substantial amounts of screening test, and the bar cloth patch being finally made, its drugloading rate is big, and bioavilability is high, and treatment bone is closed
The scorching correlation highly significant of section, tests convenient and simple.
To further illustrate beneficial effects of the present invention, the present invention has also listed following test example, it is intended to illustrate the present invention
Beneficial effect, but be in no way intended to limit present disclosure：
First, the screening of prescription：
Prescription of the invention is that plus-minusization is carried on the basis of classical " the stifling proved recipe of seedling doctor ", and the present invention is respectively with this
The taste medicine prescription of invention prescription 12, subtract ministerial drug group, subtract adjutant group, subtract make medicine group be made bar cloth paste, carry out pharmacodynamics investigation, investigate
2012-2016, No. 2 Affiliated Hospital of Guiyang College of Traditional Chinese Medicine's Orthopaedics Clinic and unit patients, after diagnosing for Femoral head is bad
Extremely, and meet the patient of inclusive criteria, four groups of normal therapeutic group and bar cloth patch group are randomly divided into by hospital order.Normal therapeutic group
Therapeutic scheme：Drip-feed complex bone peptide 6ml/d and Breviscapinun 60ml/d, oral Xianlinggubao, 3 pieces/times, 3 times/d, treatment
After 3 weeks, rest 1 week, continuous treatment 3 months.Ba Bu patch group therapeutic schemes：Jia Babu patches are controlled on the basis of normal therapeutic scheme
Treat, 1 time/d, treatment time is with normal therapeutic group.Patient clinical evaluation table and rank criterion pair are applied before and after treatment respectively
It carries out comprehensive assessment, the clinical efficacy of induction and contrast bar cloth patch.
Result shows：Contrasted with normal therapeutic group：12 herbal medicine prescription acceptance rates of the invention improve 43%, subtract ministerial drug group
Acceptance rate improves 15%, and it is 20% to subtract adjutant group acceptance rate, and subtracting makes medicine group acceptance rate be 22%, and curative effect of the present invention is substantially better than it
His group, illustrate prescription of the present invention more it is rigorous rationally, drug effect is most strong.
2nd, bar cloth patch craft screening：
In Chinese medical extract bar cloth patch preparation process of the present invention, preferable bar cloth patch matrix is filtered out using prior art and is matched somebody with somebody
Side is prepared, and carries out quality evaluation：
Test group 1:It is NP700 to use matrix formulations:Crosslinking agent [AL (OH) 3:EDTA-2Na:ALCL3]:Glycerine=0.75
(0.02:0.01:0.02):10 are prepared.
Test group 2：Using matrix formulations Carbomer:Sodium Polyacrylate:PVP K30:Carboxymethylcellulose calcium
Sodium:Polyvinyl alcohol=3.0:4.0:0.5:0.2:0.5 is prepared.
Test group 3：By using matrix formulations：Gelatin 0.8g, Sodium Polyacrylate 0.6g, titanium dioxide 0.8g, glycerine 16g,
Sodium carboxymethylcellulose 0.6g, polyvinylpyrrolidone 3g, tartaric acid 0.15g, Dihydroxyaluminium Aminoacetate 0.05g are prepared.
Test group 4：Using matrix formulations：Polyvinylcaprolactame-polyvinyl acetate-polyethyleneglycol-graft copolymer
(soluplus) 0.8g, gelatin 0.8g, Sodium Polyacrylate 0.6g, titanium dioxide 0.8g, glycerine 16g, sodium carboxymethylcellulose
0.6g, polyvinylpyrrolidone 3g, tartaric acid 0.15g, Dihydroxyaluminium Aminoacetate 0.05g are prepared.
Investigate evaluation method：With initial adhesion force, hold viscous force, sensory evaluation (appearance character, skin-friendliness, lotion residual,
Skin tracing ability, repeatedly noticing property) its bar cloth exchange premium row is commented for evaluation index.Last synthesis indices obtain comprehensive obtaining
Point, and the quality that compound osteitis bar cloth is pasted is obtained with fraction height evaluation different substrates, it is shown in Table 1.
The compound osteitis bar cloth of table 1 pastes quality evaluation
| Evaluation index | Method | Index score value |
| Initial adhesion force | 《Chinese Pharmacopoeia》The E emplastrums of annex Ⅻ stick the method for amylograph first | 0~25 |
| Hold viscous force | 《Chinese Pharmacopoeia》The E emplastrums of annex Ⅻ stick the method for amylograph second | 0~25 |
| Appearance character | Outward appearance is homogeneous, invariably dissolved particles, bubble | 0~10 |
| Skin-friendliness | It is neither too hard, nor too soft not tight, it is nonirritant | 0~10 |
| Lotion residual | After noticing, cream face keeps complete, skin noresidue | 0~10 |
| Skin tracing ability | After being affixed in wrist, firmly get rid of 10 times and do not fall | 0~10 |
| Noticing property repeatedly | After noticing repeatedly, appoint and so keep good stickiness | 0~10 |
Investigate result such as table 2 below：
Table 2 investigates result of the test
| Test group | Initial adhesion force | Hold viscous force | Sensory evaluation | Comprehensive grading |
| Test group 1 | 18 | 17 | 35 | 70 |
| Test group 2 | 20 | 20 | 36 | 76 |
| Test group 3 | 22 | 22 | 40 | 84 |
| Test group 4 | 24 | 23 | 47 | 94 |
Result of the test shows:The present invention filters out preferable bar cloth patch matrix formulations and is prepared bar in selection prior art
Cloth pastes (test group 1 and test group 2), and its bar cloth patch quality evaluation total score is relatively low, and bar cloth patch is second-rate, and the present invention is preferably
It is obtained and is obviously improved compared with test group 1-2 the matrix (test group 3) for going out, but effect is still not ideal enough, dissolution in vitro experiment knot
Fruit is only 72%；To be well positioned to meet the requirement of bar cloth patch, the present invention is analyzed research carrying out extract, unexpected to send out
It is existing, after extract drying and crushing is crossed into 200 mesh sieves, and Polyvinylcaprolactame-polyvinyl acetate is added in bar cloth patch matrix
After ester-polyethyleneglycol-graft copolymer (soluplus), it greatly improves the dissolution rate of bar cloth patch, dissolution in vitro experiment knot
Fruit is 92%, and the patch integrated survey of obtained bar cloth is evaluated as 94 points, best in quality.
3rd, compound osteitis bar cloth patch pharmacodynamics and correlative study：
(1) compound osteitis bar cloth patch anti-inflammatory experimental study
1 experiment material
1.1 medicines
Osteitis obtained in trial drug embodiment of the present invention 1-5 disappears, 3cm × 3cm；Positive control medicine：Cubic board muscles and bones
Pain patch is produced by Henan Province four directions medicine company Group Co., Ltd, lot number 1260019, and specification is 7cm × 10cm；Function and master
Control：Alleviate caput femoris necrosis, osteoproliferation, cervical spondylopathy, lumbar vertebra disease, disc herniation, rheumatic arthritis, tatanic vertebra
Inflammation, scapulohumeral periarthritis, bruise damage the pain that hair wound, Senile osteoarthritis etc. cause, and promote its rehabilitation.
1.2 experimental animals
Kunming mouse, male and female half and half, body weight 18-22g, credit number SCXK (Chongqing) 20120006.Regular grade wistar
Male rat 30, body weight 200-250g, credit number SCXK (Chongqing) 20070006, signs and issues unit：Chongqing City's experimental animal inspection
Measured center.
1.3 experiment reagents and instrument
Vulcanized sodium：Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd, lot number 20120221 (is matched somebody with somebody with 75% ethanol before use
It is set to 8% solution)；Dimethylbenzene Chongqing Mao Ye chemical reagent Co., Ltd lot number 20130508；Card punch (6mm), timer,
Electronic balance；Micrometer callipers.
2 experimental techniques
2.1 compound osteitis bar cloth paste the influence of paraxylene induced mice auricle swelling degree
Take 40 small white mouses and be randomly divided into 5 groups, every group 8 (male and female half and half) test first 24 hours with 8% vulcanized sodium belly
Unhairing, excipient control group：30% ethanol 0.3ml/ is only administered；Positive controls：The cubic each 3cm × 3cm of board muscles and bones pain patch；
Compound osteitis bar cloth pastes high dose group：Often patch gives bar cloth patch obtained in the embodiment of the present invention 5；Bone anti-inflammatory bar cloth pastes middle dosage：
Give the embodiment of the present invention and bar cloth patch obtained in 2-4 is obtained；Ba Bu pastes low dose group：Give bar obtained in the embodiment of the present invention 1
Cloth is pasted.Once a day, for three days on end, the daily belly of each group removes medicine after being administered 6 hours.After last dose 1 hour, applied to mouse right ear
With dimethylbenzene 0.05ml/ only, left ear is compared.Cervical vertebra is taken off after 30 minutes and puts to death mouse, cut left and right auricle auricle, use diameter
6mm card punch takes the circular auricle at same position from two auricles respectively, weighs, and is referred to as swelling using two auricle weight differences
Mark, the difference between comparative drug group and excipient control group, and obtain inhibiting rate.
Swelling (mg)=auris dextra piece weight (mg)-left auricle weight (mg)
Swelling inhibiting rate (%)=(the average average swelling of the administration group of swelling one of excipients group)/excipients group averagely swells
Expansibility x100%.
2.2 compound osteitis bar cloth paste the influence to swelling degree of the paw
Healthy wistar male rats 30 are taken, 5 groups, every group 6, excipient control group are randomly divided into：30% ethanol
0.3ml/ is only administered；Positive controls：The cubic each 3cm × 3cm of board muscles and bones pain patch；Compound osteitis bar cloth pastes high dose group：Often
Patch gives bar cloth patch obtained in the embodiment of the present invention 5；Bone anti-inflammatory bar cloth pastes middle dosage：Give the embodiment of the present invention and 2-4 systems are obtained
The bar cloth patch for obtaining；Ba Bu pastes low dose group：Give bar cloth patch obtained in the embodiment of the present invention 1.Once a day, for three days on end, each group
Daily belly removes medicine after being administered 6 hours.Every Rat Right metapedes thickness is determined within 3rd day as the normal value before modeling, last is given
After medicine 1 hour, the right fresh albumen 0.1ml of metapedes plantar subcutaneous injection 10% of each group animal every, to cause acute pedal swelling mould
Type, interior micrometer callipers measures the right same part thickness of metapedes when 0.5,1,2,4 and 6 hours after causing inflammation.Each rat causes
Vola pedis difference in diameter is swelling before and after scorching, using swelling as Testing index.Compare administration group and excipients control group swelling
The difference of degree, carries out statistical analysis.
Vola pedis diameter before vola pedis diameter-cause is scorching after swelling (mm)=cause inflammation.
3 data processings and statistical method
Experimental data is represented with mean ± standard deviation (Mean ± SD), and uses SPSS (Statistical Product
And Service Solutions, SPSS) 19.0 statistical analysis softwares carry out Mathematical Statistics Analysis, and compound osteitis bar cloth patch is right
In the experiment of dimethylbenzene induced mice auricle swelling degree influence, to each group experimental data first with Shapiro-Wilk methods and
Kolmogrov-Smitnov methods carry out test of normality, then carry out homogeneity of variance analysis with Levene methods, if meeting normal distribution
And homogeneity of variance, then multi-group data is compared and use variance analysis, compare two-by-two between recombinating using LSD-t inspections more, difference shows
The analysis of work property takes α=0.05, P<0.05 represents statistically significant.Swelling degree of the paw is influenceed to test in compound osteitis bar cloth patch
In, the variance analysis of multigroup Repeated Measurements data, P are used to each group experimental data<0.05 represents statistically significant.
4.1 compound osteitis bar cloth paste the influence of paraxylene induced mice auricle swelling degree
In anti-inflammatory experiment, the middle dose group and mice auricle swelling caused by positive controls paraxylene of bar cloth patch are equal
There is certain inhibitory action, it is statistically significant with excipient control group comparing difference.The middle dose group anti-inflammatory of its mini-bus cloth patch
Effect is more than positive controls, shows the middle dosage of bar cloth patch and can substantially resist the inflammatory reaction caused by dimethylbenzene.It is shown in Table 3.
The compound osteitis bar cloth of table 3 patch paraxylene induced mice auricle edema influence (N=8)
Note：Each group is made comparisons with excipient group, * P ﹤ 0.05.
4.2 compound osteitis bar cloth paste the influence to swelling degree of the paw
Under the inspection level of α=0.05, sphericity test result is unsatisfactory for (P<0.01) it is, therefore special using Spss19.0 duplicate measurements
Variance analysis is carried out to data information (table 2) with module.Huynh-Feldt assays show：Treatment Effects and time effect
Reciprocation it is not statistically significant (F=1.891, P=0.52), time main effect statistically significant (F=67.832, P<
0.0001), treatment main effect also statistically significant (F=5.009, P<0.01), effect comparative result table two-by-two between each group group
It is bright：Ba Bu pastes low dose group and excipient control group no significant difference (P>0.05), bar cloth patch middle dose group and figuration
Agent control group comparing difference has statistical significance (P<0.05), bar cloth patch middle dose group and positive controls and excipient control
Group comparing difference is respectively provided with statistical significance (P<0.01), show that middle dose group has substantially to rat paw edema caused by egg
Inhibitory action, and better than low dose group high.It is shown in Table 4
The compound osteitis bar cloth of table 4 paste to rat paw swelling induced by egg degree (mm) influence (N=8)
Paw swelling：Between F groups=5.009, P ﹤ 0.01；F time=67.832, P ﹤ 0.01；F interacts=1.891, P ＞
0.05.(2) compound osteitis bar cloth patch analgesia effect research
1 experiment material
1.1 medicines
1 experiment material
1.1 medicines
Osteitis obtained in trial drug embodiment of the present invention 1-5 disappears, 3cm × 3cm；Positive control medicine：Cubic board muscles and bones
Pain patch is produced by Henan Province four directions medicine company Group Co., Ltd, lot number 1260019, and specification is 7cm × 10cm；Function and master
Control：Alleviate caput femoris necrosis, osteoproliferation, cervical spondylopathy, lumbar vertebra disease, disc herniation, rheumatic arthritis, tatanic vertebra
Inflammation, scapulohumeral periarthritis, bruise damage the pain that hair wound, Senile osteoarthritis etc. cause, and promote its rehabilitation.
1.2 experimental animals
Kunming mouse 40, male and female half and half, body weight 18-22g signs and issues unit：Experimental animal inspection center of Chongqing City.Perhaps
Can the number of card SCXK (Chongqing) 20120006.
1.3 experiment reagents
Vulcanized sodium：Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd, lot number 20120221 (is matched somebody with somebody with 75% ethanol before use
It is set to 8% solution)；Glacial acetic acid：Chinese medicines group chemical experiment Co., Ltd, lot number T20110106.CJ-8402 types hot plate is surveyed
Pain instrument, Zhejiang Ninghai Bai Shi electromedicines instrument plant.
2. experimental technique
Influence of the 2.1 bar cloth patch to mouse writhing reaction
Small white mouse 40 is taken, packet, topical application of drug method and time test with anti-inflammatory.After topical remedy being cleaned with warm water, every
The glacial acetic acid 0.1ml10g-1 of mouse peritoneal injection 0.6%, (acetum prepared before use) induces writhing response, record note
The writhing number of times of each mouse in 15 minutes after induced pain is penetrated, each administration group compares with excipient group.
2.2 compound osteitis bar cloth paste the influence to mouse hot-plate induced pain
Some female small white mouses are taken, by mouse by being only placed on (55 ± 0.5) DEG C hot-plate instrument, the pain of record mouse is determined
Threshold value, to lick metapedes as pain sensation index, selects the mouse 40 in 5~30s of pain threshold, is randomly divided into 5 groups, every group 8.Then
It is each to survey 2 threshold of pains (interval 10min) by being only placed on hot-plate instrument, average before being administered as mouse Basic Pain Threshold just
The normal threshold of pain.Each group mouse is administered with 8% vulcanized sodium belly unhairing, and the daily belly that starts respectively, and excipient control group presses 30%
Ethanol 0.3ml/ is only administered；Positive controls are each 3cm × 3cm patches of cubic board muscles and bones pain patch；
The pain threshold of mouse is determined after last dose 30min, as pain threshold after administration.If mouse is in 60s on hot plate
Still analgesia reaction, is calculated and counted the threshold of pain raising value before and after administration by 60s.
Percentage (%)=(threshold of pain-Basic Pain Threshold after administration)/Basic Pain Threshold × 100% that the threshold of pain is improved
3 data processings and statistical method
Experimental data is represented with mean ± standard deviation (Mean ± SD), and uses SPSS (Statistical Product
And Service Solutions, SPSS) 19.0 statistical analysis softwares carry out Mathematical Statistics Analysis, to each group experimental data elder generation
Test of normality is carried out with Shapiro-Wilk methods and Kolmogrov-Smitnov methods, then homogeneity of variance is carried out with Levene methods
Analysis, if meeting normal distribution and homogeneity of variance, compares multi-group data and uses variance analysis, compares two-by-two between recombinating more and adopts
Checked with LSD-t, significance difference analysis take α=0.05, P<0.05 represents statistically significant.
4 results
Influence of the 4.1 compound osteitis bar cloth patch to mouse writhing reaction
In analgesic experiment, the basic, normal, high dosage group and excipient group comparing difference of compound osteitis bar cloth patch, with system
Meter learns meaning (p ＜ 0.05).Show that each group can resist the pain reaction caused by glacial acetic acid stimulates mouse, make mouse writhing number of times
Significantly reduce, and middle dose group effect of the present invention is substantially optimal.It is shown in Table 5.
The compound osteitis bar cloth of table 5 paste to mouse analgesic activity influence (writhing method) (N=8)
| Group | n | Writhing number of times in 15min |
| Excipients control group | 8 | 40.88±3.36 |
| Positive controls | 8 | 23.75±2.49* |
| Ba Bu pastes high dose group | 8 | 28.25±2.12* |
| Ba Bu pastes middle dose group | 8 | 19.38±2.67* |
| Ba Bu pastes low dose group | 8 | 29.50±2.07* |
Note：Each group is made comparisons with excipient group, * p ﹤ 0.05.
4.2 compound osteitis bar cloth paste the influence to mouse hot-plate induced pain
In analgesic experiment, height, middle dose group after administration compare with excipient group, can improve mouse pain threshold, poor
It is different to learn the ﹚ of Yi Yi ﹙ P ﹤ 0.05 with Tong meters.And the middle dose group analgesic activity of bar cloth patch is most strong.Ba Bu pastes high, middle dose group and oneself
Compare before body administration, can in various degree improve the pain threshold of mouse, there were significant differences, Yi Yi ﹙ P ﹤ are learned with Tong meters
0.05 ﹚.It is shown in Table 6.
The compound osteitis bar cloth of table 6 paste to mouse analgesic activity influence (hot plate method) (N=8)
Note：Each group is made comparisons with excipient group：* P ﹤ 0.05；Compare with pain threshold before administration：△ P ﹤ 0.05.
(3) foundation of rabbit Osteoarthritis model and compound osteitis bar cloth patch influence on tectology
1 experiment material
1.1 experimental animals
Choose healthy adult new zealand white rabbit 70, male and female half and half, 2.0 ± 0.5kg of body weight.Sign and issue unit：Chongqing City
Experimental animal inspection center.Credit number SCXK (Chongqing) 20120006.
1.2 experiment conditions are controlled
Laboratory room temperature is maintained at 26 DEG C or so, and humidity 50%-60% keeps ventilation, each group rabbit during experiment
Single single cage, normal daylighting.
1.3 feeds
The Special feed for rabbits provided by Guiyang College of Traditional Chinese Medicine animal is fed, free water.
1.4 medicines and major experimental equipment
1. vulcanized sodium：Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd, lot number 20120221 (uses 75% ethanol before use
It is configured to 8% solution).
2. compound osteitis bar cloth is pasted：Bar cloth patch obtained in embodiment of the present invention 1-5.By Guiyang College of Traditional Chinese Medicine's preparation laboratory
There is provided, often paste specification and be 3cm × 3cm.
3. compound osteitis bar cloth pastes bare substrate, mainly by water, sodium carboxymethylcellulose, NP-700, glycerine, azone, cunning
Stone flour, gelatin etc. reconcile be applied on non-woven fabrics in proportion, are placed in air blast freeze-day with constant temperature machine and somewhat dry so that preservative film is covered
It is translucent shape milky into, the compound osteitis bar cloth that is made patch matrix.Often patch specification is 3cm × 3cm.
4. positive control medicine：Cubic board muscles and bones pain patch is produced by Henan Province four directions medicine company Group Co., Ltd, lot number
1260019, specification is 7cm × 10cm：Function with cure mainly：Alleviate caput femoris necrosis, osteoproliferation, cervical spondylopathy, lumbar vertebra disease, vertebra
The pain that disk protrusion, rheumatic arthritis, ankylosing spondylitis, scapulohumeral periarthritis, traumatic injury, Senile osteoarthritis etc. cause
Bitterly, its rehabilitation is promoted.
5. rabbit-hutch box is provided by animal institute of Guiyang College of Traditional Chinese Medicine laboratory.
6. HH-W digital displays thermostat water bath.
7. B05002584y fish dives ultrasonic atomizer
8. embedding machine.
9. CELLSENS STANDAR D biological micro-image analyzers
10. LEICA RM2235 slicers.
2 experimental techniques
2.1 experimental animals are grouped
Healthy new zealand white rabbit 70.Male and female half and half, 2.0 ± 0.5kg of body weight is randomly divided into 7 groups.Every group 8.It is divided into
(model group 10) the 1. Normal group 2. model group 3. positive controls four directions board muscles and bones pain patch 4. stifling group of seedling doctor 5. compound
6. compound osteitis bar cloth pastes middle dose group 7. compound osteitis bar cloth patch middle dose group to osteitis bar cloth patch high dose group.
2.2 experimental animal models make
In addition to normal group, since remaining each group took left hind ankle-joint to stretch a gypsum testing first week by the 6th week
Cast is fixed, Cast length be upper end close in shin bone upper 1/3 at, at lower end to foot nearly 1/3, persistently fix 6 weeks.
After 6 weeks, model group puts to death 2, takes articular cartilage, observes gross specimen and cartilage pathological change situation, and mark of dividing right and left is put
Enter 10% neutral formalin liquid and fix to be detected.To understand joint braking effect.After modeling terminates, plaster fixing is removed, its
Remaining (in addition to normal group) takes left hind ankle-joint cartilage for sample carries out pathological observation after 6 weeks treat.
2.3 each group medications
Normal group：Normal raising is not administered.
Model group：Give compound osteitis bar cloth patch bare substrate.
Positive controls：Give the cubic each 3cm × 3cm of board muscles and bones pain patch.
Seedling cures fumigation and steaming therapy group：Seedling medicine concentrate is given daily stifling once, each half an hour.
Stifling method is as follows：
1. stifling concentrate is put into the container of powder charge agent of B05002584y fish dive ultrasonic atomizers, and spraying
Pipe is put into water-bath.
2. the temperature control of water of HH-W digital display thermostat water baths is made at 60 DEG C.
3. allow spraying pipe by being connected with rabbit-hutch box after water-bath, and make the temperature control of medicine steam 35 DEG C~40
℃。
4. experimental animal rabbit is put into rabbit-hutch box, fumigates half an hour.Cotton is covered above rabbit box, prevents medicine steam from expanding
Dissipate, in order to avoid influence therapeutic effect.
Compound osteitis bar cloth pastes high dose group：Give bar cloth patch obtained in the embodiment of the present invention 5.
Compound osteitis bar cloth pastes middle dose group：Give bar cloth patch obtained in embodiment of the present invention 2-4.
Compound osteitis bar cloth pastes low dose group：Give bar cloth patch obtained in the embodiment of the present invention 1.
Above each group is administered once a day, and even applies 6 hours, successive administration 6 weeks.
2.4 observation indexs
2.4.1 systemic conditions observation
The state of mind of observation experiment animal, active situation, feed, row are rushed down, trichomadesis, whether there is body surface infection, survival
Situation etc., experimental animal is weighed weekly once, with the change of observation experiment the weight of animals.
2.4.2 the observation of gross specimen
All animals put to death experimental animal after 6 weeks last dose fasting 12h are treated with auricular vein air embolism method.
Hair, sterilization are cut off in tested ankle, skin, fascia superficialis, deep fascia is cut successively with scalpel, ankle-joint is manifested
The change substantially such as chamber, thickness of form, color and luster and cartilage of detect by an unaided eye articular cavity and articular cartilage surface.Joint is soft
Bone gross examination of skeletal muscle reference literature standard is scored：0 point：Articular surface is smooth, and color and luster is normal；1 point：Articular surface is coarse, there is crack,
Color and luster is gloomy；2 points：Articular surface is rotten to the corn, and cartilage defect reaches cartilage table, middle level；3 points：Articular surface ulcer is formed, and cartilage defect reaches soft
Bone deep layer；4 points：Cartilage is stripped off, subchondral bone exposure.
2.4.3 chondropathology observation
Materials are pinpointed at experimental side articular surface of ankle cartilage with scalpel, as deep as subchondral layer, and is trimmed to
0.5cmx0.3cmx 0.2cm sizes, after being cleaned with 0.9% physiological saline, are placed in 10% neutral formic acid solution and fix one
My god, fixed cartilage is through decalcification one month (changing liquid once in 3 days) under 10% EDTA ﹙ PH=7 ﹚ normal temperature.Sample routine Tuo Shui ﹙ 70%
Alcohol one and a half hours → 80% alcohol 1 hour 20 minutes → 90% the alcohol of alcohol 1 hour → 95% 1. 45 minutes →
95% alcohol 2. 45 minutes → 100% alcohol 1. 30 minutes → 100% alcohol 2. 30 minutes, form a gradient Tuo Shui ﹚；It is conventional
Bone tissue is respectively put into 100% alcohol by Tou Ming ﹙：Dimethylbenzene is 1：1 20 minutes → dimethylbenzene of solution 1. 30 minutes → diformazan
Benzene 2. 30 Fen Zhong ﹚；Conventional Jin La ﹙ are respectively by bone tissue successively immersion wax cylinder 1. 1 hour → wax cylinder 2. 1 little Shi ﹚；Routine paraffin wax bag
Mai ﹙ first make 4um thickness serial section, numbering, then row Hematoxylin-eosin (HE, Hematoxylin-Eos in) dyeing respectively, most
It is complete afterwards to show cartilage full Ceng ﹚.The pathological change of articular cartilage is scored by Mankin ' S standards of grading, every section emphasis
Observe institutional framework, cell quantity, the damp line integrality of articular cartilage.Institute's score value is higher, shows that lesion degree is more serious.See
Table 7.
Table 7Mankin ' S standards of grading
2.4.4 bone histomorphometric research
According to cartilage pathological section, cartilage thickness is observed with CELLSENS STANDAR D biological micro-images analyzer,
Every section randomly selects 4 visuals field, measures and shoot the photo of each group ankle-joint cartilage thickness.
2.4.5 bone histomorphometric research
Every cartilage slices take 4 high power field of view (200 times) at random, wherein 2 are located at cartilage top layer and layer of dividing a word with a hyphen at the end of a line, separately
2 are located at cartilage radiating layer and calcified layer, notice that sample should choose neighbouring regression defect, but with complete cartilage structure
4 layers of region be observed.Calculate 4 layers of sum of region cartilage cell.
3 data processings and statistical method
All of above experimental data is represented with mean ± standard deviation (Mean ± SD), and uses SPSS (Statistical
Product and Service Solutions, SPSS) 19.0 statistical analysis softwares carry out Mathematical Statistics Analysis, to each group reality
Test data first carries out test of normality with Shapiro-Wilk methods and Kolmogrov-Smitnov methods, then is carried out with Levene methods
Homogeneity of variance is analyzed, if meeting normal distribution and homogeneity of variance, multi-group data is compared and uses variance analysis, two between recombinating more
Two compare using LSD-t inspections, and significance difference analysis take α=0.05, P<0.05 represents statistically significant.
4 results
4.1 overview results：
Animal normal group state of mind during testing is good, is quick on the draw, and freely, hair color is glossy for ankle moving.
Each group after modeling has limping in various degree, and ankle moving is substantially limited, One's spirits are drooping, and gloss is poor.Compound osteitis bar
Cloth patch height, middle dose group are by after treatment, degree has been alleviated, and middle dosage effect is the most notable.
The substantially visual results of 4.2 rabbit ankle-joint samples：
Normal group：Articular cartilage surface is smooth, neat in edge, is creamy white, and cartilage transparency is higher, flawless and
Defect, synovia is limpid, amount is few.
Model control group：Articular cartilage surface is rough, and color and luster is dim, in lark, there is obvious rimala；Greatly
Small, the form and shallow Bu mono- ﹐ cartilages of Shen are thinning, fragmentation.Synovial membrane visible hyperemia and oedema；Joint fluid increases, and color is yellowish.
The stifling group of seedling doctor：Articular surface is in pale, and surface is still smooth, and part sample surface has crackle to be formed.
Compound osteitis bar cloth pastes low dose group：Articular cartilage surface part tarnishes, slightly lark, has and significantly splits
Gap, cartilage wear degree slightly mitigates compared with model control group.
Compound osteitis bar cloth pastes middle dose group and positive controls：Articular cartilage is in yellow-white, and surface portion loses normally
Gloss, color and luster is slightly dark, smoother, has no obvious aggressivity cartilage defect.It is unobvious difference to be visually observed between two groups.Two groups
Articular cartilage outward appearance and color are better than model group.The results are shown in Table 8.
The substantially grade form (n=4) of articular cartilage during 8 each group of table 6 weeks
| Group | Number of animals | 0 point | 1 point | 2 points | 3 points | 4 points |
| Normal group | 4 | 4 | 0 | 0 | 0 | 0 |
| Model group | 4 | 0 | 0 | 1 | 1 | 2 |
| Positive controls | 4 | 0 | 1 | 2 | 1 | 0 |
| The stifling group of seedling doctor | 4 | 0 | 1 | 1 | 2 | 0 |
| Ba Bu pastes high dose group | 4 | 0 | 1 | 2 | 1 | 0 |
| Ba Bu pastes middle dose group | 4 | 0 | 1 | 2 | 2 | 0 |
| Ba Bu pastes low dose group | 4 | 0 | 0 | 1 | 2 | 1 |
4.3 light microscope articular cartilages observe result：
Normal group：Articular cartilage structure is normal, and cell arrangement is neat, and nucleus is in navy blue, endochylema pinkiness.Top layer
Cartilage cell is in fusiformis, like fibrocyte；Intermediate layer cell is rounded, arranges irregular；Columnar cell's layer is in the form of a column arrangement, can
See damp line.Cartilage matrix uniform coloring, pinkiness.
Model control group：Cartilage thickness is thinning, and cell quantity is reduced, and arrangement disorder, crack is more, partly reaches calcification
Layer, especially based on cells of superficial layer reduction, and has more inflammatory cell to cover, and cartilage inner cell density is uneven, and damp line is disorderly.
Positive controls：Cartilage structure is present, and cell arrangement is neat, and damp line is clearer.Cartilage matrix uniform coloring, in powder
It is red.
Middle dose group：Cartilage matrix coloring is more uniform, and cell layering is clear in order, and cellular morphology is relatively regular, and damp line is more clear
Chu.
High and low dose group：There is a small amount of crack in cartilage surface, partly reaches radiating layer.Cell lacks unity and coherence, and arranges nothing
Rule, damp line obscures imperfect.But situation is better than model group.
The stifling group of seedling doctor：Cartilage matrix coloring is more uniform, and cell arrangement is in the form of a column, and level is still clear, cellular morphology rule,
Damp line is more visible.The results are shown in Table 9.
Each group cartilage Mankin ' the S appraisal results of table 9 (N=4)
| Group | n | Average integral (divides) |
| Model group | 4 | 10.00±0.82★ |
| Positive drug group | 4 | 6.00±0.82▲ |
| The stifling group of seedling doctor | 4 | 7.25±0.96▲★ |
| Compound osteitis bar cloth pastes high dose group | 4 | 6.25±0.96▲ |
| Compound osteitis bar cloth pastes middle dose group | 4 | 5.25±0.96▲ |
| Compound osteitis bar cloth pastes low dose group | 4 | 8.75±0.96▲★ |
Note：Each group compares ▲ p ﹤ 0.05 with model group；Compare ★ p ﹤ 0.05 with positive drug group.
4.4 cartilage thickness statistics
Each group compares with model group, there is the notable ﹚ of Cha Yi ﹙ p ﹤ 0.05.Middle dose group and positive controls, compare without having
Significant difference (p ﹤ 0.05), shows the middle dose group of compound osteitis bar cloth patch on Osteoarthritis articular cartilage thickness is prevented and treated
It is more excellent with positive controls therapeutic action.The results are shown in Table 10.
The ankle-joint cartilage thickness statistical form of table 10 (N=4)
Note：Each group is made comparisons with model group, ▲ p ﹤ 0.05；Compare with positive drug group, ◆ p ﹤ 0.05.
4.5 cartilage cell's counting statistics tables
The low dose group of compound osteitis bar cloth patch compares with model group, without the notable poor different ﹚ of ﹙ p ＞ 0.05.Other each groups and mould
Type group compares, and has the notable ﹚ of Cha Yi ﹙ p ﹤ 0.05.Middle dose group compares with the ankle-joint cartilage cell of positive controls.Show multiple
On Osteoarthritis energy is prevented and treated, increasing treated with positive controls in cartilage cell's quantity for the middle dose group of quadrate bone inflammation bar cloth patch
Effect compares more excellent.The results are shown in Table 11.
The cartilage cell's counting statistics table of table 11 (N=4)
Note：Each group is made comparisons ▲ p ﹤ 0.05 with model group；Compare with positive drug group, ◆ p ﹤ 0.05.
(4) discuss
It is to smear to cause scorching medicine-dimethylbenzene, left ear to compare, cause by auris dextra that the auricle oedema of mouse causes scorching experiment
Mice Auricle part capillary injection, permeability increases, oozes out and increase, and oedema occurs, and shows the diseases such as red, swollen, hot, pain
Shape.The swelling degree of the paw of rat causes scorching experiment to be injected in rat paw by egg white, can cause topical acute inflammation, makes part
Tissue edema.Suppression of the observation medicine to rear paw swelling.It is a kind of modeling method of acute inflammation.The hot-plate of mouse is
When being subject to hot plate to stimulate by the foot of mouse and producing pain, just add sufficient special reaction.To contact hot plate to adding foot
Afterwards the required time as pain threshold, as observation index, compare the threshold of pain value changes between medication group and control group to test
Demonstrate,prove the analgesic activity of medicine.The writhing assay of mouse is a kind of sensitive, simple, reproducible of screening NSAIDs effect
Method, the model is applied to maincenter and periphery antalgesic and studies.This experiment causes abdominal cavity to be held by mouse peritoneal injection acetic acid
Long pain reaction, causes mouse " writhing response " occur.Many studies have shown that in the analgesic activity using the scale-model investigation medicine
When medicine action intensity and clinical analgesic effect there is obvious correlation.Selected 4 inflammation of this research, analgesic mould
Type, is checking medicine anti-inflammatory, the classical trials method of analgesic activity, can serve as research compound osteitis bar cloth patch anti-inflammatory, town
The effect of pain.
Result of the test shows：The excellent positive controls of effect is significant of the invention, treat Osteoarthritis effect highly significant,
And better than low dose group high, illustrate that currently preferred compound osteitis bar cloth patch Chinese medicine preparation and matrix formulations are scientific and reasonable.
4th, compound osteitis bar cloth patch toxicology and correlative study
1 materials and methods
1.1 materials
Health Wistar rats 72 are chosen, (experimental animal rises the limited public affairs of prosperous biotechnology to albino guinea-pig 30 by Chongqing
Department provides), self-control compound osteitis bar cloth patch, self-control compound osteitis bar cloth patch matrix,
1.2 methods
Health Wistar rats 30 are selected in acute toxicity test, and male and female half and half, body weight 200-250g is randomly divided into two
Group, A groups 20, B groups 10.24h is lost hair or feathers with 8% vulcanized sodium to rat back before experiment.A groups stick heavy dose of compound osteitis bar
Cloth is pasted, and B sticks compound osteitis bar cloth patch matrix, and application time is 24h.Medicine is washed after 24h, the index observing by table one is big
Mouse 2 weeks.
The reaction of animals index of the acute toxicity test of table 12 observation
Skin irritation test selects the natural rats of Wisar 42, and male and female half and half, body weight 200-250g is randomly divided into 7
Group, intact skin high dose group, intact skin middle dose group, intact skin low dose group, damaged skin high dose group, damaged skin
Skin middle dose group, damaged skin low dose group, negative control group, every group 6.8% vulcanized sodium of 24h is to rat back before experiment
Depilation.24h is lost hair or feathers with 8% vulcanized sodium to rat back before experiment.Successive administration 14 days, observes 30-60min after last dose,
24h, 48h, 72h local skin stimulate situation.Scored by the standard of table two, table three is evaluated.
The skin wound repair standards of grading of table 13
The skin irritatin intensity evaluation standard of table 14
The healthy albino guinea-pig 30 of skin active hypersensitive test selection, male and female half and half, 6-8 week old, body weight 250-300g, with
Machine is divided into 3 groups, every group 10.24h is lost hair or feathers with 8% vulcanized sodium to cavy rib belly before experiment.Test group, negative control group are
Only, for 0.2ml/ only, the 0th, 7,14 days sensitization phases and stimulating phase are respectively administered once the positive group 0.2g/ on the 28th day.Test group gives multiple
Quadrate bone inflammation bar cloth patch, negative control group gives compound osteitis bar cloth patch matrix, and positive controls give DNFB, cause
Quick phase concentration is 1%, and stimulating phase concentration is 0.1% (being configured with 70% ethanol).It is administered at the left side depilation of sensitization phase, stimulating phase is right
It is administered at the depilation of side.Observation 6,24,48,72h after exciting.Scored by the standard of table four, table five is evaluated.
The standards of grading of the cutaneous anaphylaxis of table 15
The evaluation criterion of the cutaneous anaphylaxis of table 16
2 results
Acute toxicity test test during each group animal general behavior sign, at administration local skin is normal, do not observe
To the part and general toxic reaction of rat；The big dosis tolerata of group that rat disappears to osteitis is more than 21.5g/kg.
Skin irritation test test during each group animal general behavior sign, local skin is showed no exception at administration,
Damaged skin does not see the irritative responses such as obvious hydroderma and erythema with intact skin group；Commented according to skin irritation
Price card is accurate, and skin irritatin evaluation result is shown in Table six, and compound osteitis bar cloth patch does not have excitant to rat skin.Show compound osteitis
Ba Bu patches are damaged to rat and intact skin is without obvious irritation.But long-term continuous use may have one to wound healing
It is fixing to ring.
The compound osteitis bar cloth lagging skin results of stimulation of table 17
During cavy active skin anaphylactic test, office at test group and negative control group animal general behavior sign, administration
Portion's skin is showed no exception, and positive controls animal general behavior sign is normal, the appearance erythema of local skin difference depth, even
There is incrustation phenomenon.Appraisal result is shown in Table after seven compound osteitis bar cloth patch is repeatedly sticked with guinea pig skin and does not find after a cavy not
There are the allergic reactions such as obvious erythema and oedema, the opposite all cavys of positive controls are after excitation by different degrees of
Erythema and oedema even form a scab reaction occur, dermoreaction integration be 5.9, sensitization rate be 100%, be extreme sensitization
Property；Show compound osteitis bar cloth patch to guinea pig skin without obvious sensitization.
The compound osteitis bar cloth of table 18 pastes cavy active skin anaphylactic test appraisal result
3 discuss
By acute toxicity test, skin irritation test, three researchs of toxicology test of skin active hypersensitive test
Result shows that compound osteitis bar cloth patch belongs to external preparation for skin medicine, is only limited to be used on skin, and percutaneous dosing toxicity is very low, whether
To intact skin or damaged skin all without toxic reaction, animal systemic physiological function is not influenceed, be a kind of security
The externally applied drug for the treatment of Osteoarthritis higher.
Specific embodiment：
Embodiment 1：The preparation of the bar cloth patch that osteitis disappears
(1) rhizome of davallia 4g, jatamans valeriana rhizome 4g, bark of eucommia 4g, scythian lamb rhizome 4g, toddalia 4g, reticulate millettia 4g, yellow angledtwig magnoliavine root stem and leaf are weighed
4g, Heiguteng exract 4g, HERBA EPIMEDII 4g, deep fragrant 4g, lycopodium calvatum 4g, sargentodoxa cuneata 4g, plus the decocting of 8 times of amounts are boiled 2 times, and 1.5 is small every time
When, filtrate is reclaimed in filtering, and concentration dry, pulverize 200 mesh sieves, and obtain final product Chinese medical extract.
(2) accurately weighed Sodium Polyacrylate 4g, titanium dioxide 6g, are mixed uniformly, add glycerine 130g, and grinding is equal
It is even, as A phases.Accurately weighed sodium carboxymethylcellulose 4g, gelatin 6g, polyvinylpyrrolidone 18g, polyethylene acyl in oneself again
Amine-polyvinyl acetate-polyethyleneglycol-graft copolymer (soluplus) 6g is mixed uniformly, is adding water 45g, and oil bath adds
Enter to make it fully swelling, as B phases.A, B two-phase are ground, 0.1 times of traditional Chinese medicine extraction of amount step (1) of matrix is added
Thing, tartaric acid 0.8g and Dihydroxyaluminium Aminoacetate 0.1g, are well mixed.Coated on backing, dry and be obtained bar cloth patch.
Embodiment 2：The preparation of the bar cloth patch that osteitis disappears
(1) rhizome of davallia 5g, jatamans valeriana rhizome 5g, bark of eucommia 5g, scythian lamb rhizome 5g, toddalia 5g, reticulate millettia 5g, yellow angledtwig magnoliavine root stem and leaf are weighed
5g, Heiguteng exract 5g, HERBA EPIMEDII 5g, deep fragrant 5g, lycopodium calvatum 5g, sargentodoxa cuneata 5g, plus the decocting of 7 times of amounts are boiled 2 times, and 1.5 is small every time
When, filtrate is reclaimed in filtering, and concentration dry, pulverize 200 mesh sieves, and obtain final product Chinese medical extract.
(2) accurately weighed Sodium Polyacrylate 5g, titanium dioxide 7g, are mixed uniformly, add glycerine 140g, and grinding is equal
It is even, as A phases.Accurately weighed sodium carboxymethylcellulose 5g, gelatin 7g, polyvinylpyrrolidone 20g, polyethylene acyl in oneself again
Amine-polyvinyl acetate-polyethyleneglycol-graft copolymer (soluplus) 6g is mixed uniformly, is adding water 50g, and oil bath adds
Enter to make it fully swelling, as B phases.A, B two-phase are ground, 0.1 times of traditional Chinese medicine extraction of amount step (1) of matrix is added
Thing, tartaric acid 1g and Dihydroxyaluminium Aminoacetate 0.1g, are well mixed.Coated on backing, dry and be obtained bar cloth patch.
Embodiment 3：The preparation of the bar cloth patch that osteitis disappears
(1) rhizome of davallia 8g, jatamans valeriana rhizome 8g, bark of eucommia 8g, scythian lamb rhizome 8g, toddalia 8g, reticulate millettia 8g, yellow angledtwig magnoliavine root stem and leaf are weighed
8g, Heiguteng exract 8g, HERBA EPIMEDII 8g, deep fragrant 8g, lycopodium calvatum 8g, sargentodoxa cuneata 8g, plus the decocting of 8 times of amounts are boiled 2 times, and 1.5 is small every time
When, filtrate is reclaimed in filtering, and concentration dry, pulverize 200 mesh sieves, and obtain final product Chinese medical extract.
(2) accurately weighed Sodium Polyacrylate 6g, titanium dioxide 8g, are mixed uniformly, add glycerine 160g, and grinding is equal
It is even, as A phases.Accurately weighed sodium carboxymethylcellulose 6g, gelatin 8g, PVPK30 g, polyethylene acyl in oneself again
Amine-polyvinyl acetate-polyethyleneglycol-graft copolymer (soluplus) 8g is mixed uniformly, is adding water 70g, and oil bath adds
Enter to make it fully swelling, as B phases.A, B two-phase are ground, 0.3 times of traditional Chinese medicine extraction of amount step (1) of matrix is added
Thing, tartaric acid 1.5g and Dihydroxyaluminium Aminoacetate 0.5g, are well mixed.Coated on backing, dry and be obtained bar cloth patch.
Embodiment 4：The preparation of the bar cloth patch that osteitis disappears
(1) weigh rhizome of davallia 10g, jatamans valeriana rhizome 10g, bark of eucommia 10g, scythian lamb rhizome 10g, toddalia 10g, reticulate millettia 10g,
Yellow angledtwig magnoliavine root stem and leaf 10g, Heiguteng exract 10g, HERBA EPIMEDII 10g, deep fragrant 10g, lycopodium calvatum 10g, sargentodoxa cuneata 10g, plus the decocting of 9 times of amounts boil 2
Secondary, 1.5 hours every time, filtrate was reclaimed in filtering, and concentration dry, pulverize 200 mesh sieves, and obtain final product Chinese medical extract.
(2) accurately weighed Sodium Polyacrylate 7g, titanium dioxide 9g, are mixed uniformly, add glycerine 180g, and grinding is equal
It is even, as A phases.Accurately weighed sodium carboxymethylcellulose 7g, gelatin 9g, PVP g, polyethylene acyl in oneself again
Amine-polyvinyl acetate-polyethyleneglycol-graft copolymer (soluplus) 9g is mixed uniformly, is adding water 90g, and oil bath adds
Enter to make it fully swelling, as B phases.A, B two-phase are ground, 0.5 times of traditional Chinese medicine extraction of amount step (1) of matrix is added
Thing, tartaric acid 2g and Dihydroxyaluminium Aminoacetate 1g, are well mixed.Coated on backing, dry and be obtained bar cloth patch.
Embodiment 5：The preparation of the bar cloth patch that osteitis disappears
(1) weigh rhizome of davallia 11g, jatamans valeriana rhizome 11g, bark of eucommia 11g, scythian lamb rhizome 11g, toddalia 11, reticulate millettia 11g,
Yellow angledtwig magnoliavine root stem and leaf 11g, Heiguteng exract 11g, HERBA EPIMEDII 11g, deep fragrant 11g, lycopodium calvatum 11g, sargentodoxa cuneata 11g, plus the decocting of 9 times of amounts boil 2
Secondary, 1.5 hours every time, filtrate was reclaimed in filtering, and concentration dry, pulverize 200 mesh sieves, and obtain final product Chinese medical extract.
(2) accurately weighed Sodium Polyacrylate 9g, titanium dioxide 11g, are mixed uniformly, add glycerine 190g, grind
Uniformly, as A phases.Accurately weighed sodium carboxymethylcellulose 8g, gelatin 10g, polyvinylpyrrolidone 45g, polyethylene are in oneself again
Acid amides-polyvinyl acetate-polyethyleneglycol-graft copolymer (soluplus) 10g is mixed uniformly, is adding water 100g, oil
Bath addition makes it fully swelling, used as B phases.A, B two-phase are ground, the Chinese medicine for adding 0.6 times of amount step (1) of matrix is carried
Thing, tartaric acid 2.2g and Dihydroxyaluminium Aminoacetate 1.1g are taken, is well mixed.Coated on backing, dry and be obtained bar cloth patch.
Claims (6)
1. a kind of compound osteitis bar cloth is pasted, it is characterised in that：It is made up of following matrix and Chinese medical extract：
Matrix：By weight, by Polyvinylcaprolactame-polyvinyl acetate-polyethyleneglycol-graft copolymer
(soluplus) 7-9 parts, gelatin 7-9 parts, Sodium Polyacrylate 5-7 parts, titanium dioxide 7-9 parts, sodium carboxymethylcellulose 5-7 parts,
Polyvinylpyrrolidone 20-40 parts, tartaric acid 1-2 parts, Dihydroxyaluminium Aminoacetate 0.1-1 parts, glycerine 140-180 parts, distilled water 50-90 parts of system
Into；
Chinese medical extract：By weight, by rhizome of davallia 5-10 parts, jatamans valeriana rhizome 5-10 parts, bark of eucommia 5-10 parts, scythian lamb rhizome 5-10
Part, toddalia 5-10 parts, reticulate millettia 5-10 parts, yellow angledtwig magnoliavine root stem and leaf 5-10 parts, Heiguteng exract 5-10 parts, HERBA EPIMEDII 5-10 parts, deep perfume (or spice)
5-10 parts, lycopodium calvatum 5-10 parts, sargentodoxa cuneata 5-10 parts is made.
2. compound osteitis bar cloth according to claim 1 is pasted, it is characterised in that：By following matrix and Chinese medical extract system
Into：
Matrix：By weight, by Polyvinylcaprolactame-polyvinyl acetate-polyethyleneglycol-graft copolymer
(soluplus) 8 parts, 8 parts of gelatin, 6 parts of Sodium Polyacrylate, 8 parts of titanium dioxide, 6 parts of sodium carboxymethylcellulose, polyvinyl pyrrole
30 parts of alkanone, 1.5 parts of tartaric acid, 0.5 part of Dihydroxyaluminium Aminoacetate, 160 parts of glycerine, 70 parts of distilled water are prepared from；
Chinese medical extract：By weight, by 8 parts of the rhizome of davallia, 8 parts of jatamans valeriana rhizome, 8 parts of the bark of eucommia, 8 parts of scythian lamb rhizome, toddalia 8
Part, 8 parts of reticulate millettia, 8 parts of yellow angledtwig magnoliavine root stem and leaf, 8 parts of Heiguteng exract, 8 parts of HERBA EPIMEDII, deep fragrant 8 parts, 8 parts of lycopodium calvatum, 8 parts of sargentodoxa cuneata is through carrying
Take and form.
3. compound osteitis bar cloth according to claim 1 and 2 is pasted, it is characterised in that by matrix and the matter of Chinese medical extract
Amount is than being 10:1-5 is made.
4. compound osteitis bar cloth according to claim 1 and 2 is pasted, it is characterised in that by matrix and the matter of Chinese medical extract
Amount is than being 10:3 are made.
5. compound osteitis bar cloth according to claim 1 and 2 patch, it is characterised in that described Chinese medical extract be by with
What lower step was prepared from：Weigh the rhizome of davallia, jatamans valeriana rhizome, the bark of eucommia, scythian lamb rhizome, toddalia, reticulate millettia, yellow angledtwig magnoliavine root stem and leaf, black bone
Rattan, HERBA EPIMEDII, deep perfume, lycopodium calvatum, sargentodoxa cuneata, plus the 7-9 times of decocting measured are boiled 2 times, and 1.5 hours every time, filter was reclaimed in filtering
Liquid, concentration, dry, pulverize 200 mesh sieves, obtain final product Chinese medical extract.
6. it is a kind of to treat the preparation method that compound osteitis bar cloth is pasted, it is characterised in that to comprise the following steps：
Accurately weighed Sodium Polyacrylate, titanium dioxide is mixed uniformly, adds glycerine, and grinding is uniform, used as A phases；It is smart again
Close weighed sodium carboxymethylcellulose, gelatin, polyvinylpyrrolidone, Polyvinylcaprolactame-polyvinyl acetate-polyethylene glycol
Graft copolymer (soluplus) is mixed uniformly, is adding water, and oil bath is added makes it fully swelling, used as B phases；By A, B
Two-phase is ground, and adds tartaric acid and Dihydroxyaluminium Aminoacetate, adds Chinese medical extract, is well mixed；Coated on backing, dried in the air
It is dry to obtain bar cloth patch.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108652809A (en) * | 2018-03-27 | 2018-10-16 | 李秀荣 | Orthopaedics intelligence brace |
| CN110151951A (en) * | 2019-07-15 | 2019-08-23 | 河南白马健康产业有限公司 | One seedling medicine muscles and bones leads to waist patch and preparation method thereof |
| CN112057512A (en) * | 2020-10-19 | 2020-12-11 | 吴言发 | Medicine for treating muscle and bone pain |
-
2017
- 2017-03-30 CN CN201710204257.2A patent/CN106822377B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 徐剑等: "正交试验优化骨炎消巴布剂的基质处方", 《中国实验方剂学杂志》 * |
| 徐航: "苗医熏蒸疗法对早期股骨头坏死的疗效观察", 《万方》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108652809A (en) * | 2018-03-27 | 2018-10-16 | 李秀荣 | Orthopaedics intelligence brace |
| CN110151951A (en) * | 2019-07-15 | 2019-08-23 | 河南白马健康产业有限公司 | One seedling medicine muscles and bones leads to waist patch and preparation method thereof |
| CN112057512A (en) * | 2020-10-19 | 2020-12-11 | 吴言发 | Medicine for treating muscle and bone pain |
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Address after: No. 50, Shidong Road, Nanming District, Guiyang City, Guizhou Province Patentee after: Guizhou University of Traditional Chinese Medicine Address before: No. 50, Nanming District, Nanming District, Guiyang, Guizhou Patentee before: GUIYANG College OF TRADITIONAL CHINESE MEDICINE |
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Application publication date: 20170613 Assignee: First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine Assignor: Guizhou University of Traditional Chinese Medicine Contract record no.: X2021520000001 Denomination of invention: A compound Guyan cataplasm and its preparation method Granted publication date: 20200925 License type: Common License Record date: 20210305 |