CN106819481B - Complete fermented feed for piglet creep and preparation method thereof - Google Patents
Complete fermented feed for piglet creep and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a complete fermented feed for piglet creep, which solves the technical blank of related complete fermented feeds in the prior art. The complete fermented feed is prepared by fermenting a fermentation base material with a composite viable bacteria preparation; the fermentation base material is prepared from the following raw materials in percentage by weight: 4-6% of fermented soybean meal, 8-12% of puffed soybean, 10-14% of soybean meal, 1-3% of plasma protein, 2-4% of soybean oil, 30-36% of corn, 18-22% of puffed corn, 8-12% of whey powder and 4-6% of premix; the composite viable bacteria preparation consists of lactobacillus plantarum, pediococcus pentosaceus PP, bacillus subtilis, saccharomyces boulardii, saccharomyces cerevisiae, candida utilis and clostridium butyricum. The complete fermentation feed disclosed by the invention is fragrant in fermentation, and can be used for remarkably improving the feed attractant and bringing forward the starter period of piglets; the palatability can be obviously improved, the food intake is promoted, the food intake is increased, and the digestion, absorption and utilization rate of the feed can be obviously improved; and the intestinal flora can be optimized, the diarrhea of piglets can be prevented and treated, and the diarrhea rate can be effectively reduced.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a complete fermented feed for piglet creep and a preparation method thereof.
Background
The production of piglets is a key link with great technical difficulty and complexity in the pig industry, the diarrhea of piglets is always the first killer in the pig industry, and particularly, the diarrhea is more obvious and prominent in cold and humid weather, so that great economic loss is brought to breeders. The problems of yellow-white dysentery and diarrhea of piglets, the stress problem of 'opening period transition' from milk eating to feeding, and the stress problem of weaning are all worldwide problems in piglet production and are also serious disasters of antibiotic abuse. At present, the use of antibiotics is more and more strictly controlled all over the world, and except the use of antibiotics, the piglet diarrhea is mainly prevented and treated in a nutrition regulation mode in production. The nutrition regulation and control modes mainly comprise: regulating protein, energy, fiber content, adding additives, adding digestive aid, adding immunopotentiator, etc.
However, at present, the use amount of the unprocessed soybean meal in most piglet feeds is relatively high (except for fermented soybean meal or puffed soybean), and the antigen component in the soybean protein is a main cause of piglet intestinal tract injury and piglet post-weaning diarrhea. The existing method for solving the problem is to partially reduce antigen components in protein through soybean meal or diet processing, such as puffing, hot ethanol leaching and the like, but the effect is limited or chemical residues are possibly generated; secondly, the dosage of soybean products is limited, the dosage of the soybean meal which is not processed in the daily ration of the general recommended weaned piglet is not too high, and then the requirements of the piglets on the protein are met by adding other protein raw materials, such as spray-dried blood powder, spray-dried plasma protein powder, milk powder, fish meal and the like, wherein the price of the milk replacer for the piglets is high due to the addition of the protein raw materials, so that the breeding cost investment is greatly increased; and thirdly, the protein level of the daily ration is reduced, in order to prevent diarrhea of the piglets, the protein content of some creep feed in the market is even as low as 15% -16%, so that the weaned weight of the piglets does not reach the standard, the production performance of the piglets is seriously reduced, and the production performance of subsequent nursing and growing fattening is reduced. The addition of acidifying agent, enzyme preparation, immunopotentiator, etc. in the daily ration has certain effect of preventing diarrhea of piglets. However, the acidifier is mainly simple organic acid such as acetic acid, lactic acid and the like, and the enzyme preparation, the immunopotentiator and the like have certain loss after the feed process, so that the effect is difficult to ensure.
In recent years, the application of fermented feed and fermented feed raw materials in preventing and treating piglet diarrhea is increasingly wide. The fermented soybean meal, bran and other feed raw materials can improve the nutrient digestion and utilization rate of the feed raw materials and reduce anti-nutritional factors and toxins. However, the efficacy of single raw material fermentation still has limitations, for example, other feed raw materials still have the problems of anti-nutritional factors and even toxin exceeding standard. The existing fermented feed is only added into complete feed according to a very low proportion, for example, only 4% -10%. Because the addition amount of the fermented feed is small, the influence on the growth performance of the piglets is mainly determined by complete feed, and the efficacy of the fermented feed is difficult to guarantee and exert to the greatest extent.
At present, there are some reports on fermented complete feeds, such as: the invention patent (patent application No. CN201410258693.4) produces complete feed suitable for various animals by fermentation, but does not fully consider the digestive physiological characteristics and nutrient requirements of different animals, and the strain preparation process is complex, the culture medium cost is high, the fermentation process needs stirring, the fermentation needs drying, the fermentation needs to be mixed with other complete materials or other raw materials for use, the procedures are various, and the popularization is not facilitated. The invention patent CN105410410A discloses a complete fermented feed without resistance and low mycotoxin, which is prepared by adding a certain proportion of molasses for the growth of microorganisms and fermenting corn, bean pulp, bran and the like by using the compound microorganisms; however, the fermented complete feed mainly aims at growing and fattening pigs and pregnant sows.
The 2 weeks before and 2 weeks after the piglets are critical periods for the growth of the piglets, wherein the 2 weeks before the piglets are weaned is the opening transition period from the feeding of the piglets to the feeding of the piglets, and the stress problem of the transition of the opening period exists; the problem of weaning stress exists in 2 weeks after the piglets are weaned, so that 2 weeks before and 2 weeks after the piglets are weaned are the high incidence period of piglet diseases, particularly the occurrence of diarrhea, and the complete fermented feed aiming at the special period is not reported at present.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a complete fermented feed for piglet creep and a preparation method thereof. The invention takes complete and balanced nutrition antibiotic-free complete compound feed as a fermentation base material, adds a compound viable bacteria preparation, and prepares the compound viable bacteria compound feed after fermentation. The complete fermented feed can not only fully meet the nutritional requirements of animals, but also increase various bioactive enzymes and functional and nutritional probiotics such as organic acid, vitamin, amino acid, peptide and the like, and is rich in a large amount of probiotics; the feed additive has the biological effects of remarkably improving the feed attraction and palatability, promoting the ingestion, effectively improving the digestion, absorption and utilization rate, optimizing intestinal flora, improving immunity, resisting stress, preventing diseases, promoting growth and the like, saving the production cost and improving the production benefit.
In order to achieve the purpose, the invention adopts the following technical scheme:
a complete fermented feed for piglet creep is prepared by fermenting fermented base material with composite viable bacteria preparation;
the fermentation base material is prepared from the following raw materials in percentage by weight:
4-6% of fermented soybean meal, 8-12% of puffed soybean, 10-14% of soybean meal, 1-3% of plasma protein, 2-4% of soybean oil, 30-36% of corn, 18-22% of puffed corn, 8-12% of whey powder and 4-6% of premix.
The composite viable bacteria preparation is prepared from the following components in percentage by weight: m2010150 lactobacillus plantarum LP with the preservation number of CCTCC NO: m2012029 Pediococcus pentosaceus PP with a preservation number of CCTCC NO: m2010260, Bacillus subtilis B7348 with preservation number of CCTCC NO: m2011301 Bacillus subtilis N9, Saccharomyces boulardii, Saccharomyces cerevisiae, CCTCC NO: m2010090 Candida utilis and CCTCC NO: c.butyricum Cb-2 composition of M2011384.
Preferably, the fermentation base material is prepared from the following raw materials in percentage by weight:
5% of fermented soybean meal, 10% of puffed soybean, 12% of soybean meal, 2% of plasma protein, 3% of soybean oil, 33% of corn, 20% of puffed corn, 10% of whey powder and 5% of premix.
Preferably, the composite viable bacteria preparation is prepared from lactobacillus plantarum LP, pediococcus pentosaceus PP, bacillus subtilis B7348, bacillus subtilis N9, saccharomyces boulardii, saccharomyces cerevisiae, candida utilis and clostridium butyricum Cb-2 in a mass ratio of 10: 10: 1: 1: 1: 1: 1: 0.4.
The invention also provides a preparation method of the complete fermented feed for piglet creep, which comprises the following steps:
(1) adding water into the fermentation base material and the composite viable bacteria preparation, uniformly mixing, adjusting the water content to 18-22% and the pH value to 6.5-7.5, and preparing a mixture to be fermented;
(2) and (3) carrying out aerobic fermentation on the mixture to be fermented, and then carrying out anaerobic fermentation to prepare the complete fermentation feed.
In the step (1), the viable count of the lactobacillus plantarum LP and the pediococcus pentosaceus PP in the mixture to be fermented is not less than 1 × 107The number of viable bacteria of cfu/g, Bacillus subtilis B7348, Bacillus subtilis N9, Saccharomyces boulardii, Saccharomyces cerevisiae, Candida utilis and Clostridium butyricum Cb-2 is not less than 1 × 106cfu/g。
In the step (2), the aerobic fermentation conditions are as follows: the water content is 20%, the fermentation time is 12h, and the temperature is 25 ℃; starting the fermentation of spore bacteria, filamentous fungi and yeast.
In the step (2), the anaerobic fermentation conditions are as follows: the water content is 20%, the fermentation time is 24h, and the temperature is 20 ℃; starting the fermentation of clostridium butyricum and lactobacillus.
The application of the complete fermented feed in serving as a piglet creep feed is also within the protection scope of the invention.
The invention has the beneficial effects that:
(1) aiming at the special growth period of 2 weeks before and 2 weeks after the weaning of the piglets, the invention designs the complete fermented feed for the piglet creep, the complete fermented feed does not contain antibiotics, can not cause burden on the liver and kidney metabolism of the piglets, and avoids the defect that the antibiotics inhibit the later growth vigor of the piglets. And the fermentation efficacy of the fermented feed is superior to that of a single fermented raw material and a fermented feed with functional addition. The complete fermented feed has the advantages of having fermented fragrance, being capable of obviously improving the feed attractant and bringing forward the starter of piglets; after fermentation, palatability can be obviously improved, feed intake is promoted, feed intake is increased, and the digestion, absorption and utilization rate of feed can be obviously improved; meanwhile, the complete fermented feed disclosed by the invention can optimize intestinal flora, prevent and treat piglet diarrhea and effectively reduce the diarrhea rate. Improving immunity, resisting stress, and promoting health of piglets, thereby increasing rapidly and stably.
(2) Compared with the microbial fermented feed disclosed in patent CN105475624A, the complete fermented feed needs to provide all nutritional requirements and energy requirements for the piglets in a special growth period of 2 weeks before and 2 weeks after weaning, so that the design requirements for the components of the fermented base material are stricter and more targeted; in addition, the piglet is easy to cause diarrhea in the period, and aiming at the problem, the composition of the composite viable bacteria preparation for fermentation is optimized, the saccharomyces boulardii and the beer yeast are added, the using amount of each component bacterium in the composite viable bacteria preparation is adjusted, and the control effect on the piglet diarrhea is effectively improved.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application.
FIG. 1: the feed intake of each pig in the experiment control group and the experiment group is equal by feeding the complete fermentation creep feed.
FIG. 2: the weight of each pig in the experiment control group and the experiment group is equal to that of the pigs in the experiment group.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
Description of terms:
complete fermented feed: the feed is prepared by adding a special fermented composite viable bacteria preparation for the feed and a proper amount of water into antibiotic-free complete compound feed with comprehensive and balanced nutrition, fully and uniformly mixing, and performing a certain aerobic, anaerobic or organic combined fermentation process flow under the regulation and control of proper temperature, pH value and the like.
Premixing: is the abbreviation of additive premix, which is an important component of complete compound feed. It is an intermediate compound feed product prepared by mixing one or more trace components (including various trace mineral elements, various vitamins, synthetic amino acids, certain medicinal additives and the like) with a diluent or a carrier according to a required ratio and uniformly mixing. The premix of the present invention is a commercially available product which is conventional in the art.
As described in the background art, there is no report on a complete fermented feed for a specific period of 2 weeks before weaning and 2 weeks after weaning of piglets in the prior art, and in order to solve the above technical problems, the present application proposes a complete fermented feed for piglet creep, specifically as follows:
the complete fermented feed is prepared by fermenting a fermentation base material with a composite viable bacteria preparation;
the fermentation base material is prepared from the following raw materials in percentage by weight:
4-6% of fermented soybean meal, 8-12% of puffed soybean, 10-14% of soybean meal, 1-3% of plasma protein, 2-4% of soybean oil, 30-36% of corn, 18-22% of puffed corn, 8-12% of whey powder and 4-6% of premix.
The composite viable bacteria preparation is prepared from the following components in percentage by weight: m2010150 lactobacillus plantarum LP with the preservation number of CCTCC NO: m2012029 Pediococcus pentosaceus PP with a preservation number of CCTCC NO: m2010260, Bacillus subtilis B7348 with preservation number of CCTCC NO: m2011301 Bacillus subtilis N9, Saccharomyces boulardii, Saccharomyces cerevisiae, CCTCC NO: m2010090 Candida utilis and CCTCC NO: c.butyricum Cb-2 composition of M2011384.
Wherein the preservation number is CCTCC NO: the lactobacillus plantarum LP of M2010150 has been described in the patent "a lactic acid bacterium useful for biological preservation and its application" (CN 101914475A).
The preservation number is CCTCC NO: m2012029 Pediococcus pentosaceus PP has been described in the patent "a lactic acid bacteria semisolid fermentation product and process for improving animal growth performance" (CN 102599348A).
The preservation number is CCTCC NO: clostridium butyricum Cb-2 of M2011384 has been described in the patent "A strain of Bacillus cereus acidogenic and applications thereof" (CN 102517238A).
The preservation number is CCTCC NO: the bacillus subtilis B7348 of M2010260 has been described in the patent "a bacillus subtilis with strong bacteriostatic action and its application" (CN 102120975A).
The preservation number is CCTCC NO: m2011301 Bacillus subtilis N9 has been described in the patent "a Bacillus subtilis strain bred by aerospace breeding technology and its application" (CN 102329749A).
The preservation number is CCTCC NO: the candida utilis of M2010090 is already described in the patent "a yeast strain with strong organic selenium tolerance, enrichment and transformation capability and its application" (CN 101864369A).
The above-mentioned deposited strains were purchased from bio-engineering Co., Ltd, Bao Lai, Shandong; saccharomyces boulardii powder was purchased from santa sachusensis bio ltd, Shandong; saccharomyces cerevisiae was purchased from Bio-engineering GmbH, Bay, Shandong.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and commercially available.
Example 1: preparation of complete fermented feed
1. Composition of composite live bacteria preparation and fermentation base material
Specifically, the results are shown in Table 1.
Table 1: composition of composite live bacteria preparation and fermentation base material
2. The preparation method comprises the following steps:
(1) adding water into the fermentation base material and the composite viable bacteria preparation, uniformly mixing, adjusting the water content to 20% and the pH value to 6.5-7.5, and preparing a mixture to be fermented;
(2) firstly, carrying out aerobic fermentation on a mixture to be fermented, wherein the aerobic fermentation conditions are as follows: the water content is 20%, the fermentation time is 12h, and the temperature is 25 ℃; starting fermentation of spore bacteria, filamentous fungi and yeast; after the aerobic fermentation is finished, adjusting the water content to be 20%, and then carrying out anaerobic fermentation for 24 hours at the temperature of 20 ℃ to prepare the complete fermented feed.
According to the method, the water content in the fermentation system is regulated, so that the fermentation process can be regulated; on the other hand, the prepared complete fermented feed has moderate water content, and the problems of storage and application caused by excessive water content in the fermented feed are avoided.
Comparative example 1:
the composite viable bacteria preparation of example 1 was removed, and the fermentation base material of example 1 was used as a raw material composition to prepare a general complete feed by a conventional method.
Comparative example 2:
the "saccharomyces boulardii" and "saccharomyces cerevisiae" in the composite viable bacteria preparation of example 1 were removed, and a complete fermented feed was prepared by the method of example 1 in the same manner as in example 1.
Application example 1:
test site: the Shandong province Taian city of Ningyang county drink a certain scale of pig farm.
Test time: year 2016, 03/08 to year 2016, 05/09.
1. And (3) experimental design:
(1) selecting pigs, weighing, grouping and pre-feeding: selecting 6 piglets with the same variety, health, similar weight and nest weight, and 3 nests of piglets within 2 weeks of the birth day, and optionally 1 nest as a control group; the remaining 2 litters were the experimental group for 2 replicates, 1 litter per replicate, 6 litters 1, and 10 litters 2. Pre-fed for three days.
(2) Formal experiments: the experimental period is scheduled to be two weeks before weaning and two weeks after weaning, and is 4 weeks, the control group is fed with the complete feed prepared in the comparative example 1, and the experimental groups (experimental litter 1 and experimental litter 2) are fed with the complete fermented feed prepared in the example 1, and the complete fermented feed is freely eaten and freely drunk. The experimental period was 28 days. Every morning 07: 00 am, 11 noon: feeding at 00 o 'clock and 17:00 o' clock at night (specifically, according to the condition, less feeding and adding, weighing record). And weighing and counting the feed before feeding, and weighing the residual material after material replacement. And weighing the feed and the pigs every week, and calculating feed intake, daily gain and the like. Health conditions of piglets, such as diarrhea, cough, etc., were observed during the experiment and recorded. And collecting feces samples respectively on 7 th day, 14 th day, 21 th day and 28 th day of the experimental period, counting live bacteria of lactobacillus, bacillus, enterococcus faecalis, saccharomycetes and escherichia coli, and simultaneously measuring nutritional indexes such as crude protein, crude fat, crude fiber, total energy, moisture, pH and the like in the feces samples. Before the experiment, the nutritional indexes such as crude protein, crude fat, crude fiber, total energy and the like in the feed are measured.
(3) And (3) hygiene and epidemic prevention: cleaning the pigsty 7-8 points earlier every day, removing excrement and urine and the like, and well nursing and sanitation the piglets. And the disinfection and epidemic prevention work is done by matching with the workers in the pig farm.
2. Test results and analysis:
(1) feed detection report
The control feed selected in the experiment is consistent with the complete fermented feed in the nutrition level, so the main feed raw materials selected by the control feed and the complete fermented feed are consistent, and the main difference is whether the compound viable bacteria preparation and the matched fermentation process technology in the embodiment 1 are used for fermentation. The control feed and the complete fermentation feed are produced at the same time, the same-time detection is carried out before the experiment, and the data are summarized and arranged to form a detection report shown in table 2.
As can be seen from Table 2, the complete fermented feed for the experimental group and the feed for the control group both meet the nutrition standard of the creep feed for the porkets and can meet the nutritional requirements of the porkets. The content of total acid and acid soluble protein of the complete fermented feed fed by the experimental group is obviously higher than that of the control group, and the total acid and the acid soluble protein are both probiotic nutrition and functional substances generated by probiotic fermentation. The total acid mainly comprises organic acids such as lactic acid, acetic acid, propionic acid, citric acid, succinic acid, tartaric acid, malic acid, oxalic acid, etc., and the acid soluble protein mainly comprises low molecular protein hydrolysate such as lysine, methionine, threonine, tryptophan, glutamic acid, various oligopeptides, etc. The total acid content is high, the natural acidifier is generated by metabolism of probiotics, and is beneficial to invigorating stomach and promoting appetite of porkets and helping digestion, and the metabolism mechanism of the organic acid generated by microorganisms is that polysaccharides such as starch, cellulose and the like are firstly decomposed into monosaccharides such as glucose, and then the glucose is metabolized to generate various organic acids, so that the soluble sugar content in the complete fermentation feed is high, and the complete fermentation feed has sour and sweet taste and meets the actual requirements of appetizing and invigorating stomach of porkets on enjoying sweet taste. The full-value fermented feed has stronger delicate flavor due to higher content of acid soluble protein and has certain fragrance by combining the metabolism of crude fat. Therefore, by combining the summary of the components of each substance, the complete fermented feed of the experimental group has good food calling property, early opening, good palatability and high feed intake (see experimental feed intake data).
TABLE 2 test report of complete fermented feed and control feed
(2) Feed intake
The experiment is fed on time, the feeding and the last residual materials are weighed and recorded before each feeding, the data are summarized and sorted to form a graph, and the result is shown in figure 1.
As can be seen from the figure 1, the feed intake of the piglets at 12-22 days after birth is low, the feed intake 1 week before weaning (at 22-28 days after birth) is obviously increased, and the feed intake after weaning (at 28-42 days). However, the feed intake of the complete fermented feed of the experimental group is always higher than that of the control feed of the control group in the whole experiment. Particularly after weaning, the food intake of the experimental group is obviously higher than that of the control group, and the increase speed (the slope) of the food intake of the experimental group is obviously higher than that of the control group. And it can be known from the original data (the result is shown in fig. 1), that the day of the beginning of the experiment, that is, the 11 th day after the birth of the piglet, the feed intake of the experimental group is about 5-6 g, the feed intake of the control group is about 0.4 g, the data indicates that the pigs of the experimental group really feed the complete fermented feed, while the control group is probably only good for the feed of the suckling pigs, 0.4 g of the feed is only stuck on the nose and hooves, and little or even not really feed the suckling pigs, so that the complete fermented feed has the advantages of good food calling property, early opening, good palatability and high feed intake by combining with the comprehensive analysis of the feed detection report.
(3) Only equal weight
The experiment was weighed on time, at the beginning the initial weight was weighed, at 4 weeks in the experimental period the weight was weighed weekly, at the middle 28 days of weaning the weight was weighed weaning the weight, the total weight was weighed 6 times, the data was summarized and arranged to form a graph, see figure 2.
As can be seen from fig. 2, the initial average weight of the pigs in the experimental group is slightly lower than that of the control group, the average weight of the pigs in the experimental group is always lower than that of the control group in week 1 of the experimental period, and the feed intake of the pigs in the experimental group is always higher than that of the control group, so that the breast milk in the control group is very advantageous in combination with the feed intake in week 1. In the 2 nd week of the experimental period, the weight of the experimental group only rapidly and stably increases, while the weight of the control group slightly decreases, and the combination of the feed intake and the diarrhea rate (see the feces detection report for the result for each probiotic content) shows that the control group starts to feed in the 2 nd week of the experimental period with the decrease of the amount of the breast milk, but has diarrhea, which seriously affects the growth of the suckling pigs. After the initial diarrhea, the average weight of the control group is obviously improved about 3 days before weaning, but once weaning, the average weight is seriously influenced (28-35 days old), and the weight gain is slow. Meanwhile, the experimental litter 2 is affected by weaning stress although it has almost no opening stress. However, the experimental feed nest 1 has no stress of opening and no stress of weaning, so that the complete fermented feed has obvious effects on resisting the stress of opening and weaning and preventing and treating diarrhea, thereby ensuring the healthy growth of piglets and quickly and stably increasing weight.
(4) Stool sample detection report
The experiment was performed weekly by collecting the whole group of feces samples, and the day of collection was checked for 4 times. The data of each detection is summarized and arranged to be made into a detection report which is shown in the table 3, the table 4, the table 5 and the table 6 in sequence.
The 4-time feces detection and analysis report is integrated to show that the experimental group feces probiotics are obviously higher than the control group, wherein the contents of lactic acid bacteria, bacillus and saccharomycetes in the feces of the experimental group in the 1 st, 3 th and 4 th weeks are all higher than the control group, and the content of lactic acid bacteria in the feces of the experimental group in the 2 nd week is higher than the control group. The fresh yeast No. 1 of the complete fermentation feed fed by the experimental group is mainly prepared by fermenting lactic acid bacteria, saccharomycetes and bacillus, so that the reason that the feces sample of the experimental group has higher content of probiotics is that the complete fermentation feed is eaten. Meanwhile, the disease incidence and diarrhea rate are combined with the weight of the traditional Chinese medicine, and the traditional Chinese medicine has obvious effects of reducing the disease incidence and the diarrhea rate, improving immunity and anti-stress, and stably improving daily average weight gain. In addition, different advantages are presented in different stages on other indexes of the feces. In three weeks before the experiment, the water content of the experimental group feces sample is obviously low, and the fiber digestibility is obviously higher than that of the control group, so that the diarrhea rate of the experimental group is also reflected laterally. In the fourth experiment, the contents of crude protein and crude fat of the experimental group manure sample are obviously lower than those of the control group, and the total weight gain in the fourth experiment is combined, so that the complete fermented feed can be reflected to obviously improve the digestion, absorption and utilization rate of the feed and improve the average weight gain in the stage.
TABLE 3 complete fermentation feed raising experiment 1 week fecal sample detection report
TABLE 4 complete fermented feed feeding experiment 2 week fecal sample detection report
TABLE 5 complete fermented feed feeding experiment 3 week-old fecal sample detection report
TABLE 6 complete fermented feed feeding experiment 4 week fecal sample detection report
Application example 2:
1. and (3) experimental design:
(1) selecting pigs, weighing, grouping and pre-feeding: 80 piglets with the same variety, health, similar weight and similar litter weight within 2 weeks of birth day are selected and randomly divided into an experimental group and a control group, wherein each group is provided with two parallels, and each parallels 20 piglets. Pre-fed for three days.
(2) Formal experiments: the experimental period is preset to be two weeks before weaning and two weeks after weaning, and is 4 weeks in total, the control group is fed with the full-value fermented feed prepared in the comparative example 2, and the experimental group is fed with the full-value fermented feed prepared in the example 1, and the experimental group is fed with free food intake and free drinking water. The experimental period was 28 days.
2. Measurement indexes are as follows:
determination of incidence and diarrhea rate: during the test period, the health condition of the swinery is observed and recorded at regular time every day, and the morbidity and diarrhea quantity of each parallel experiment of each group are recorded, wherein the morbidity symptoms comprise piglets suffering from cough, diarrhea and leg pain.
3. And (3) test results:
the results of the effects on morbidity and diarrhea are shown in table 7.
Table 7: the influence on the onset of disease and diarrhea
| Group of | Incidence (%) | Diarrhea Rate (%) | Mortality (%) |
| Experimental group | 8.26±0.42 | 2.21±0.16 | 0 |
| Control group | 15.56±0.53 | 4.35±0.25 | 0 |
As can be seen from table 7, no death cases were found in both the experimental group and the control group during the experimental period. Compared with the control group, the morbidity of the experimental group is reduced by 46.92%, the diarrhea rate is greatly reduced by 49.20%, and the difference is significant (P is less than 0.05). Research results show that the complete fermented feed provided by the invention is fed to the special period of 2 weeks before weaning and 2 weeks after weaning of piglets, so that diarrhea and morbidity of the piglets can be effectively reduced.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (9)
1. A complete fermented feed for piglet creep is characterized in that the feed is prepared by fermenting a fermentation base material with a composite viable bacteria preparation; the fermentation base material is prepared from the following raw materials in percentage by weight: 4-6% of fermented soybean meal, 8-12% of puffed soybean, 10-14% of soybean meal, 1-3% of plasma protein, 2-4% of soybean oil, 30-36% of corn, 18-22% of puffed corn, 8-12% of whey powder and 4-6% of premix;
the composite viable bacteria preparation is prepared from the following components in percentage by weight: m2010150 lactobacillus plantarum LP with the preservation number of CCTCC NO: m2012029 Pediococcus pentosaceus PP with a preservation number of CCTCC NO: m2010260, Bacillus subtilis B7348 with preservation number of CCTCC NO: m2011301 Bacillus subtilis N9, Saccharomyces boulardii, Saccharomyces cerevisiae, CCTCC NO: m2010090 Candida utilis and CCTCC NO: c2011384 clostridium butyricum Cb-2;
the preparation method of the complete fermented feed is characterized by comprising the following steps:
(1) adding water into the fermentation base material and the composite viable bacteria preparation, uniformly mixing, adjusting the water content to 18-22% and the pH value to 6.5-7.5, and preparing a mixture to be fermented;
(2) and (3) carrying out aerobic fermentation on the mixture to be fermented, and then carrying out anaerobic fermentation to prepare the complete fermentation feed.
2. The complete fermented feed according to claim 1, wherein the fermented base material is prepared from the following raw materials in percentage by weight: 5% of fermented soybean meal, 10% of puffed soybean, 12% of soybean meal, 2% of plasma protein, 3% of soybean oil, 33% of corn, 20% of puffed corn, 10% of whey powder and 5% of premix.
3. The complete fermented feed according to claim 1, wherein the composite viable bacteria preparation is prepared from lactobacillus plantarum LP, pediococcus pentosaceus PP, bacillus subtilis B7348, bacillus subtilis N9, saccharomyces boulardii, saccharomyces cerevisiae, candida utilis and clostridium butyricum Cb-2 in a mass ratio of 10: 10: 1: 1: 1: 1: 1: 0.4.
4. The fermented feed according to claim 1, wherein the viable count of Lactobacillus plantarum LP and Pediococcus pentosaceus PP in the mixture to be fermented in step (1) of the preparation method is not less than 1 × 107cfu/g, bacillus subtilis B7348, bacillus subtilis N9, and Brettanomyces fermentumThe viable count of mother bacteria, cerevisiae Fermentum, Candida utilis and Clostridium butyricum Cb-2 is not less than 1 × 106cfu/g。
5. The complete fermented feed according to claim 1, wherein the aerobic fermentation conditions are: the water content of the fermentation system is 20%, the fermentation time is 8-12 h, and the temperature is 15-30 ℃.
6. The complete fermented feed according to claim 5, wherein the time of aerobic fermentation is 12h and the temperature is 25 ℃.
7. The complete fermented feed according to claim 1, wherein the anaerobic fermentation conditions are: the water content is 20%; the fermentation time is 24-36 h, and the temperature is 15-25 ℃.
8. The complete fermented feed according to claim 7, wherein the anaerobic fermentation time is 24h and the temperature is 20 ℃.
9. Use of the complete fermented feed according to any one of claims 1 to 8 as a piglet creep feed.
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| CN107751541A (en) * | 2017-10-30 | 2018-03-06 | 福建傲农生物科技集团股份有限公司 | A kind of fermented feed and preparation method thereof |
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| CN109770046A (en) * | 2019-03-08 | 2019-05-21 | 刘坚 | A kind of sucking pig wean pannage and preparation method thereof |
| CN110226671A (en) * | 2019-07-19 | 2019-09-13 | 广东省农业科学院农业生物基因研究中心 | A kind of piglet full price fermented feed and its production method |
| CN111820329A (en) * | 2020-07-01 | 2020-10-27 | 安徽省天可饲料有限公司 | Fermented feed for piglets |
| CN111961611A (en) * | 2020-07-21 | 2020-11-20 | 云南省畜牧兽医科学院 | Pediococcus pentosaceus 368 and application thereof |
| CN113439799A (en) * | 2021-07-12 | 2021-09-28 | 广西壮族自治区兽医研究所 | Feed additive and preparation method and application thereof |
| CN116875483B (en) * | 2023-05-30 | 2024-04-09 | 大理州大台农台标饲料有限公司 | Composite microbial agent, fermented feed and preparation method thereof |
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