CN106818711A - A kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling - Google Patents
A kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling Download PDFInfo
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- CN106818711A CN106818711A CN201710078589.0A CN201710078589A CN106818711A CN 106818711 A CN106818711 A CN 106818711A CN 201710078589 A CN201710078589 A CN 201710078589A CN 106818711 A CN106818711 A CN 106818711A
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- middle case
- inner casing
- liquid nitrogen
- magnetic valve
- liquid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Mechanical Engineering (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling, including inner casing, shell, middle case, hydraulic pump and liquid nitrogen container；In shell, the inner casing is in middle case for the middle case；Liquid nitrogen is housed between the middle case and inner casing, some pallets are provided with the inner casing；The bottom of the inner casing is provided with the first catheter, and the catheter is provided with the first magnetic valve；The bottom of the middle case is provided with the second catheter, and second catheter is provided with the second magnetic valve, and one end of the second catheter is connected by the hydraulic pump with the liquid nitrogen container；The top of the middle case is provided with escaping pipe, and the escaping pipe is provided with the 3rd magnetic valve；Closure is provided with the opening of the middle case and inner casing, the closure is provided with controller, the signal input part of the signal output part of the controller respectively with first magnetic valve, the second magnetic valve and the 3rd magnetic valve is connected.Gas phase storage can be carried out, stored in liquid phase, and Automatic Control can be carried out again, quickly and easily can switched between two kinds of memory modules.
Description
Technical field
The invention belongs to liquid nitrogen cryogenics technical field of memory, and in particular to a kind of gas-liquid two-phase liquid nitrogen storage for automatically controlling
Device.
Background technology
For RNA, tissue, cell and precious sample, liquid nitrogen storage is undoubtedly most safe, most economical, most environmentally friendly storage
Mode.- 136 DEG C is the glass transition temperature of water, and the crystallization of water is injured to cell and tissue there is no obvious, the various lifes in sample
Change reaction almost to stop；- 196 DEG C be liquid nitrogen volatilization temperature, be the minimum temperature achieved by conventional method, the life in sample
Life activity stops substantially, and the infringement of the crystallization of water to cytoactive and tissue microstructure is almost negligible.- 136 DEG C to -196
DEG C it is temperature that gas phase liquid nitrogen container can be realized, also can for a long time stores and preserve biological specimen.Liquid nitrogen container is divided into gas phase liquid
Nitrogen tank and liquid phase liquid nitrogen container, liquid phase liquid nitrogen container easily cause booster when having requirement higher, otherwise recovery sample to storage consumptive material；
The cross-infection of sample is also easily caused simultaneously.Therefore, gas phase liquid nitrogen container does not exist above mentioned problem then, but gas phase liquid nitrogen is deposited
Storing temperature does not have the storage temperature of liquid phase liquid nitrogen low, there is presently no that can take into account liquid phase storage, can store mould at two kinds again
The automatic storage device easily and fast switched between formula.
The content of the invention
Based on this, regarding to the issue above, the present invention proposes a kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling, can
Gas phase storage is carried out, stored in liquid phase, and Automatic Control can be carried out again, quickly and easily can cut between two kinds of memory modules
Change.
The technical scheme is that：A kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling, including inner casing, shell,
Middle case, hydraulic pump and liquid nitrogen container；In shell, the inner casing is in middle case for the middle case；The middle case with
Liquid nitrogen is housed between inner casing, some pallets are provided with the inner casing；The bottom of the inner casing is provided with the first catheter, the drain
Pipe is provided with the first magnetic valve；The bottom of the middle case is provided with the second catheter, and second catheter is provided with the second electromagnetism
Valve, and one end of the second catheter is connected by the hydraulic pump with the liquid nitrogen container；The top of the middle case is provided with outlet
Pipe, the escaping pipe is provided with the 3rd magnetic valve；Closure is provided with the opening of the middle case and inner casing, the closure is provided with
Controller, the signal output part of the controller respectively with the letter of first magnetic valve, the second magnetic valve and the 3rd magnetic valve
Number input is connected.
When carrying out gas phase storage, controller controls the first magnetic valve often to close, and inner casing is in closed state, controller control
Hydraulic pump works, make liquid nitrogen in liquid nitrogen container enter in middle case, because inner casing is in closed state, the liquid nitrogen in middle case without
Method enters in inner casing, sample is placed on pallet, you can carry out gas phase pattern storage；When carrying out liquid phase mode storage, controller
The first magnetic valve is controlled to open, then the liquid nitrogen in middle case enters in inner casing, until liquid nitrogen liquid level will be placed with the pallet of sample
Submergence, stopping is passed through liquid nitrogen；When the air pressure in middle case is higher, controller controls the 3rd magnetic valve to open, you can in reduction
Between air pressure in shell.
As a further improvement on the present invention, between being additionally provided with the closure between the measurement middle case and inner casing
The electronics air gauge of the air pressure in gap, the barometric signal output part of the electronics is connected with the signal input part of the controller.
Air pressure in electronics air gauge real-time testing middle case, and measurement data is sent the data that will be measured to controller, controller
Normal value with storage is compared, if air pressure is larger, the 3rd magnetic valve of control is opened, you can automatically adjusted in middle case
Air pressure.
As a further improvement on the present invention, liquid level sensor is provided with the gap between the middle case and inner casing, should
The signal output part of liquid level sensor is connected with the signal input part of the controller.In storing process, liquid nitrogen can be by gradually
Consumption, particularly in liquid phase mode storing process, the consumption of liquid nitrogen is larger, liquid nitrogen must be in time supplemented, by liquid level sensor
Real-time testing liquid nitrogen liquid level, and test data is sent to controller, when liquid nitrogen liquid level is in below setting, control
Device controls hydraulic pump works, and liquid nitrogen is supplemented in time.
As a further improvement on the present invention, the bottom of the inner casing is provided with motor, and the output shaft of the motor is provided with and turns
Axle, the pallet is uniform successively from top to bottom and is arranged in the rotating shaft in parallel to each other；The signal output part of the controller
Signal input part with the motor is connected.When the storage of gas phase pattern is carried out, can be rotated by controller controlled motor, and then
Rotating shaft and pallet is driven to rotate, it is uniform with keeping temperature.
As a further improvement on the present invention, the gap between the shell and middle case is vacuum.Vacuum gap completely cuts off
The heat exchange of middle case and outside, better heat preservation.
The beneficial effects of the invention are as follows：
(1) gas phase storage can be carried out, stored in liquid phase can be carried out again, have concurrently gas phase storage without cross-infection, non-explosive
Pipe, and the Cord blood of stored in liquid phase advantage, and Automatic Control quickly and easily can cut between two kinds of memory modules
Change；
(2) by the air pressure in electronics air gauge real-time testing middle case, and measurement data is sent to controller, control
The data that device will be measured are compared with the normal value of storage, if air pressure is larger, the 3rd magnetic valve of control is opened, and is realized middle
Air pressure automatically adjusts in shell；
(3) by liquid level sensor real-time testing liquid nitrogen liquid level, and test data is sent to controller, works as liquid nitrogen
Liquid level is in when below setting, controller control hydraulic pump works, and liquid nitrogen is supplemented in time, realizes the monitor in real time and benefit of liquid nitrogen
Fill；
(4) when the storage of gas phase pattern is carried out, can be rotated by controller controlled motor, and then drive rotating shaft and pallet to turn
Dynamic, uniform with keeping temperature, storage effect is more preferable；
(5) gap between shell and middle case is vacuum, and vacuum gap has completely cut off the heat exchange of middle case and outside,
Better heat preservation.
Brief description of the drawings
Fig. 1 is the structural representation of the gas-liquid two-phase liquid nitrogen storage device automatically controlled described in embodiment；
Fig. 2 is the control principle drawing of each electronic component；
Description of reference numerals：
10 inner casings, 11 pallets, 12 first catheters, 13 first magnetic valves, 14 motors, 15 rotating shafts, 20 shells, in the middle of 30
Shell, 31 second catheters, 32 second magnetic valves, 33 escaping pipes, 34 the 3rd magnetic valves, 40 hydraulic pumps, 50 liquid nitrogen containers, 60 sealings
Lid, 61 controllers, 62 electronics air gauges, 63 liquid level sensors.
Specific embodiment
Embodiments of the invention are described in detail below in conjunction with the accompanying drawings.
Embodiment：
As depicted in figs. 1 and 2, a kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling, including inner casing 10, shell 20,
Middle case 30, hydraulic pump 40 and liquid nitrogen container 50；In shell 20, the inner casing 10 is located at middle case 30 to the middle case 30
It is interior；It is equipped with liquid nitrogen, the inner casing 10 between the middle case 30 and inner casing 10 and is provided with some pallets 11；The inner casing 10
Bottom is provided with the first catheter 12, and the catheter 12 is provided with the first magnetic valve 13；The bottom of the middle case 30 is provided with second
Catheter 31, second catheter 31 is provided with the second magnetic valve 32, and one end of the second catheter 31 passes through the hydraulic pump
40 are connected with the liquid nitrogen container 50；The top of the middle case 30 is provided with escaping pipe 33, and the escaping pipe 33 is provided with the 3rd electromagnetism
Valve 34；Closure 60 is provided with the opening of the middle case 30 and inner casing 10, the closure 60 is provided with controller 61, the control
The signal output part of device processed 61 respectively with the signal input of first magnetic valve 13, the second magnetic valve 32 and the 3rd magnetic valve 34
End is connected.
In another embodiment, between being additionally provided with the closure 60 between the measurement middle case 30 and inner casing 10
The electronics air gauge 62 of the air pressure in gap, the signal input part of the signal output part of the electronics air gauge 62 and the controller 61
It is connected.
In another embodiment, liquid level sensor 62 is provided with the gap between the middle case 30 and inner casing 10, should
The signal output part of liquid level sensor 63 is connected with the signal input part of the controller 61.
In another embodiment, the bottom of the inner casing 10 is provided with motor 14, and the output shaft of the motor 14 is provided with and turns
Axle 15, the pallet 11 is uniform successively from top to bottom and is arranged in the rotating shaft 15 in parallel to each other；The letter of the controller 61
Number output end is connected with the signal input part of the motor 14.
In another embodiment, the gap between the shell 20 and middle case 30 is vacuum.Vacuum gap completely cuts off
Middle case 30 and outside heat exchange, better heat preservation.
Embodiment described above only expresses specific embodiment of the invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Shield scope.
Claims (5)
1. a kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling, it is characterised in that：Including inner casing, shell, middle case, hydraulic pressure
Pump and liquid nitrogen container；In shell, the inner casing is in middle case for the middle case；It is equipped between the middle case and inner casing
Liquid nitrogen, is provided with some pallets in the inner casing；The bottom of the inner casing is provided with the first catheter, and the catheter is provided with first
Magnetic valve；The bottom of the middle case is provided with the second catheter, and second catheter is provided with the second magnetic valve, and the second drain
One end of pipe is connected by the hydraulic pump with the liquid nitrogen container；The top of the middle case is provided with escaping pipe, in the escaping pipe
It is provided with the 3rd magnetic valve；Closure is provided with the opening of the middle case and inner casing, the closure is provided with controller, the control
Signal input part of the signal output part of device processed respectively with first magnetic valve, the second magnetic valve and the 3rd magnetic valve is connected.
2. the gas-liquid two-phase liquid nitrogen storage device for automatically controlling according to claim 1, it is characterised in that：The closure
On be additionally provided with the electronics air gauge of air pressure in the gap between the measurement middle case and inner casing, the barometric signal of the electronics
Output end is connected with the signal input part of the controller.
3. the gas-liquid two-phase liquid nitrogen storage device for automatically controlling according to claim 1 and 2, it is characterised in that：In described
Between be provided with liquid level sensor in gap between shell and inner casing, the letter of the signal output part of the liquid level sensor and the controller
Number input is connected.
4. the gas-liquid two-phase liquid nitrogen storage device for automatically controlling according to claim 3, it is characterised in that：The inner casing
Bottom is provided with motor, and the output shaft of the motor is provided with rotating shaft, and the pallet is uniform successively from top to bottom and sets in parallel to each other
It is placed in the rotating shaft；The signal output part of the controller is connected with the signal input part of the motor.
5. the gas-liquid two-phase liquid nitrogen storage device for automatically controlling according to claim 1 or 2 or 4, it is characterised in that：It is described
Gap between shell and middle case is vacuum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710078589.0A CN106818711A (en) | 2017-02-14 | 2017-02-14 | A kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710078589.0A CN106818711A (en) | 2017-02-14 | 2017-02-14 | A kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106818711A true CN106818711A (en) | 2017-06-13 |
Family
ID=59129020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710078589.0A Withdrawn CN106818711A (en) | 2017-02-14 | 2017-02-14 | A kind of gas-liquid two-phase liquid nitrogen storage device for automatically controlling |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107576125A (en) * | 2017-09-15 | 2018-01-12 | 上海原能细胞生物低温设备有限公司 | Low-temperature protection device and the low temperature clamping and placing system for including it |
| CN108402032A (en) * | 2018-03-14 | 2018-08-17 | 苏杰 | A kind of cord blood stem cell storage method |
| CN108477141A (en) * | 2018-03-14 | 2018-09-04 | 苏杰 | A kind of medical hutch of cord blood stem cell |
| CN108967418A (en) * | 2018-09-09 | 2018-12-11 | 杭州紫明冷链科技有限责任公司 | A kind of memory-type liquid nitrogen fumigating instrument |
| CN111418578A (en) * | 2020-03-30 | 2020-07-17 | 北京科吉照维药剂自动化设备有限公司 | Non-contact liquid nitrogen type biological preservation container and system |
-
2017
- 2017-02-14 CN CN201710078589.0A patent/CN106818711A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107576125A (en) * | 2017-09-15 | 2018-01-12 | 上海原能细胞生物低温设备有限公司 | Low-temperature protection device and the low temperature clamping and placing system for including it |
| CN107576125B (en) * | 2017-09-15 | 2019-09-13 | 上海原能细胞生物低温设备有限公司 | Low-temperature protection device and low temperature clamping and placing system comprising it |
| CN108402032A (en) * | 2018-03-14 | 2018-08-17 | 苏杰 | A kind of cord blood stem cell storage method |
| CN108477141A (en) * | 2018-03-14 | 2018-09-04 | 苏杰 | A kind of medical hutch of cord blood stem cell |
| CN108967418A (en) * | 2018-09-09 | 2018-12-11 | 杭州紫明冷链科技有限责任公司 | A kind of memory-type liquid nitrogen fumigating instrument |
| CN111418578A (en) * | 2020-03-30 | 2020-07-17 | 北京科吉照维药剂自动化设备有限公司 | Non-contact liquid nitrogen type biological preservation container and system |
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Application publication date: 20170613 |