CN106770802A - It is a kind of to detect multivitamin method and kit in dry blood spot simultaneously - Google Patents
It is a kind of to detect multivitamin method and kit in dry blood spot simultaneously Download PDFInfo
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Abstract
Multivitamin method in dry blood spot can be simultaneously detected the invention discloses one kind, it comprises the following steps：(1) oscillation incubation after mixing dry blood spot sample and extract solution, is then centrifuged for, and takes supernatant；(2) detected using tandem mass spectrometry after step (1) gained supernatant is mixed with mobile phase and internal standard mixed liquor, by comparing the interior target ionic strength of vitamin and vitamin come the content of vitamin in drawing test serum or test plasma；In addition, the invention also discloses multivitamin kit in a kind of dry blood spot of detection simultaneously, the kit includes internal standard mixed liquor, extract solution, mobile phase and probe washing lotion.The method of the present invention can extract various water-soluble and liposoluble vitamins from dry blood spot, more than ten kind vitamins in 1 sample are quantified simultaneously in 2~3min using tandem mass spectrometer, detection cycle significantly shortens, and the testing result of vitamin has preci-sion and accuracy higher.
Description
Technical field
Multivitamin in dry blood spot can be simultaneously detected the present invention relates to vitamin detection technique, especially one kind
Method and kit.
Background technology
Vitamin (vitamin) is that humans and animals are the class that maintain normal physiological function and must be obtained from food
Organic substance, plays an important role in growth in humans, metabolic process.Body is deficient in vitamin will cause dysbolism,
And there are various diseases, this kind of disease is referred to as vitamin-deficiency.At present, vitamin can be divided into according to dissolubility fat-soluble
Vitamin and the major class of water soluble vitamin two, water soluble vitamin include B family vitamin, vitamin C etc.；Liposoluble vitamin bag
Include vitamin A. D. E, K etc..
In liposoluble vitamin, vitamin A is the main component of the photoactive substance of photosensory cell in retina, vitamin A
Shortage can cause xerophthalmia, for adult, yctalopia is easily caused during VAD；Vitamin D (VitaminD, VitD) is right
Human health particularly children's health is significant, and VitD shortage property rickets is four diseases of Chinese children keypoint control
One, clinical discovery VitD lack property rickets in addition to bone lesion, while can influence nerve, muscle, hematopoiesis and immune etc. organize
The function of organ；Vitamin E has oxidation resistant effect, can prevent the autoxidation of unrighted acid, thus has protection biological
The function of film, makes the unsaturated fatty acyl in biomembrane be not to be hardened because of oxidation, is destroyed film；Vitamin K is to normal
Blood clotting play an important role, the K that is deficient in vitamin occurs that blood clotting is slow, or even can cause massive haemorrhage.
Water soluble vitamin is mainly including B family vitamin and vitamin C etc., such as B1(thiamine), B2(riboflavin), B5
(pantothenic acid), B6(pyridoxal etc.), B12(cobalamin), nicotinic acid (Buddhist nun's theobromine), biotin, folic acid etc.；B family vitamin is in vivo
Metabolism is influenceed by constituting coenzyme, is such as played an important role in cellular respiration.
The detection of vitamin earliest period is used for general addition food, such as infant formula, cereal product and fruit juice.
European Directive 2002/46EC formulated it is a series of on food additives identify and detection added vitamin
Food in mark vitamin content regulation.5413~2010 pairs of dispensed food for baby of China's standard GB/T and dairy products
Middle vitamin content also has clearly regulation, and the method being related in standard has microbial method and high performance liquid chromatography.On dimension
The detection of raw element, conventional method is microbial method, and its sensitivity is high, result accurate, but the method there is limitations：It is whole real
Test that the cycle is long, batch testing result poor repeatability, testing result error is larger, and can only carry out single vitamin species every time
Detection；And it is nearest, the development of liquid phase tandem mass spectrum technology causes that the detection of vitamin enters a brand-new field, near several
The occupation rate of tandem mass spectrum technology medical market at home is more and more high over year so that tandem mass spectrum is in the diagnosis of clinical disease
Popularized.
It is domestic at present there was only the measure single kind vniims method for being directed to human serum, and serum is in transportation
Liquid is, it is necessary to containers hold and need to make serum stable in certain temperature range, so, some containers use glass, and its is easy
It is broken, meanwhile, the space that container takes is more；And serum is maintained in certain temperature range, it is necessary to purchase the thermostats such as refrigerator, ice
Case can also take larger space, and thus, transport serum cost is too big, and the quantity transported every time is also very limited.
The content of the invention
Based on this, providing one kind it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part can be while examines
Multivitamin method and kit in dry blood spot are surveyed, the method has preci-sion and accuracy higher, detection effect
Rate is obviously improved, and cost is lower.
To achieve the above object, the technical scheme taken of the present invention is：It is a kind of to detect various dimensions in dry blood spot simultaneously
The method of raw element, comprises the following steps：
(1) oscillation incubation after mixing dry blood spot sample and extract solution, is then centrifuged for, and takes supernatant；
(2) examined using tandem mass spectrometry after step (1) gained supernatant is mixed with mobile phase and internal standard mixed liquor
Survey, contained come vitamin in drawing test serum or test plasma by comparing the interior target ionic strength of vitamin and vitamin
Amount.It should be noted that the tandem mass spectrometry in the present invention uses Liquid Chromatography-Tandem Mass Spectrometry combined instrument.
Preferably, extract solution includes the component of volumes below percentage in the step (1)：84.75~98.95% first
Alcohol, 1~15% water and 0.05~0.25% formic acid；Or extract solution includes the component of volumes below percentage：20~
79% ethanol, 20~70% methyl alcohol and 1~10% water.
Preferably, the acquisition method of dry blood spot sample is in the step (1)：Fresh whole blood is dripped into quantitative filter paper
On piece, drop of blood is set to be evenly distributed, it is standby that filter paper dries rear sealing preserve.
Preferably, chromatographic condition in the step (2)：
Mobile phase includes the component of volumes below percentage：79.64~97.989% methyl alcohol, 2~20% water, 0.01
~0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid；Or mobile phase bag
Include the component of volumes below percentage：79.64~97.989% acetonitrile, 2~20% water, 0.01~0.35% formic acid,
And 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid at least one；Or mobile phase includes volumes below percentage
The component of ratio：49.71~84.989% methyl alcohol, 15~50% acetonitrile, 0.01~0.28% formic acid, and 0.001~
At least one in 0.01% perfluorobutyric acid and trifluoroacetic acid；
Probe washing lotion is the acetonitrile solution that volumetric concentration is 50~80%, or probe washing lotion be volumetric concentration for 50~
80% methanol aqueous solution.It should be noted that the effect of probe washing lotion be balancing side it is fixed before and after mobile phase, auxiliary measuring,
Clean probe and avoid influence of the preceding sample to rear sample measures.
Meanwhile, present invention also offers multivitamin kit in a kind of dry blood spot of detection simultaneously, including：It is interior
Mark mixed liquor, extract solution, mobile phase and probe washing lotion.
Preferably, the extract solution includes the component of volumes below percentage：84.75~98.95% methyl alcohol, 1~
15% water and 0.05~0.25% formic acid；Or extract solution includes the component of volumes below percentage：20~79% second
Alcohol, 20~70% methyl alcohol and 1~10% water.
Preferably, the mobile phase includes the component of volumes below percentage：79.64~97.989% methyl alcohol, 2~
20% water, 0.01~0.35% formic acid, and in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid at least one
Kind；Or mobile phase includes the component of volumes below percentage：79.64~97.989% acetonitrile, 2~20% water, 0.01
~0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid；Or mobile phase bag
Include the component of volumes below percentage：49.71~84.989% methyl alcohol, 15~50% acetonitrile, 0.01~0.28% first
At least one in acid, and 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid.
Preferably, the probe washing lotion is the acetonitrile solution that volumetric concentration is 50~80%, or probe washing lotion is body
Product concentration is 50~80% methanol aqueous solution.
Preferably, the internal standard mixed liquor includes vitamin B1 internal standard, folic acid internal standard, vitamin A internal standard, the dimension life of 25 hydroxyls
Plain D2 internal standards, the internal standard of 25(OH)VD 3, vitamin E internal standard and vitamin K1 internal standard.
In sum, beneficial effects of the present invention are：
(1) vitamin molecules are cracked into the method for the present invention daughter ion of different mass-to-charge ratioes by voltage, and scanning is different
The daughter ion quantity of mass-to-charge ratio, with reference to target concentration in known vitamin, calculates vitamine concentration to be measured, can simultaneously detect dry
The content of water soluble vitamin and liposoluble vitamin in blood filter paper, is domestic the first, using the method for the present invention and reagent
Box detects vitamin, and preci-sion and accuracy is higher, and is easy to transport sample to be measured, and cost is lower；
(2) method of the present invention can extract various water-soluble and liposoluble vitamins from dry blood spot, utilize
Tandem mass spectrometer is quantified simultaneously in 2~3min to more than ten kind vitamins in 1 sample, can be in human body by analysis
Vitamin level is evaluated and monitored, so as to be diagnosed to vitamin relevant disease and therapeutic effect assessment, detection cycle
Significantly shorten；
(3) whole blood sample of clinical acquisitions is made dry blood spot sample, dry blood by the method for the present invention with quantitative filter paper
Filter paper is easy to quantitative to micro sample, just coordinates the high tandem mass spectrum method of sensitivity, and dry blood spot to be easy to
Storage and transport, extract solution therein can directly make the albuminous degeneration in dry blood spot sample, it is to avoid liquid sample removes egg
White tedious steps.
Brief description of the drawings
Fig. 1 is the total ion figure of the gained of embodiment 1；
Fig. 2 is the mass spectrogram of each composition after the gained scanning of embodiment 1.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1 detects multivitamin method in dry blood spot simultaneously
First, major experimental step：
(1) internal standard working solution is prepared：In the isotope of the various vitamins provided using U.S.'s Cambridge Isotope Laboratories
Mark and concentration；According to when time amount of experimental specimen amount calculating internal standard standard working solution.Above-mentioned various internal standards are mixed to get height
Concentration internal standard mixed liquor, when using, by 1:100 ratios are diluted high concentration internal standard mixed liquor using mobile phase, note fully mixed
It is even to can obtain internal standard working solution.
(2) collection of specimens to be measured：Take fresh whole blood to drip on quantitative filter paper piece, make drop of blood NATURAL DISTRIBUTION uniform, suspension is dried in the air
It is dry to be not less than 4 hours, dry blood spot sample is obtained, sealing preserve is standby in 2~8 DEG C.
(3) process is analyzed：
1st, dry blood spot sample is taken, is beaten with perforating plier and is taken the blood cake 10~20 of 3.2 mm dias and be put into centrifuge tube, plus
Enter 200~500 microlitres of extract solution, 650~750rpm shakes frequency oscillation incubation 45 minutes at 27~30 DEG C；
2nd, taken after being centrifuged in 96 orifice plates of all supernatants additions, dry up instrument with nitrogen and dry up, separately taken 100 microlitres of mobile phases and answer
It is molten, while adding 50 microlitres of the internal standard working solution of mixing, it is covered with aluminium foil sealer and reduces volatilization, vibration is mixed；
3rd, 96 orifice plates are put into tandem mass spectrum system automatic sampler, enable application software, set up sample list, selected
Correct internal standard concentration file and acquisition method, to detect multivitamin content in dry blood spot.
(4) result is calculated：
Software can be carried out testing result by comparative analysis thing and interior target ionic strength in tandem mass spectrum system
Calculate, provide the concentration of each vitamin and generate data report.
(5) points for attention：
1st, 2~8 DEG C of Refrigerator stores should be immediately placed in after isotopic standard product are finished；
When the 2nd, wrapping up microwell plate using aluminium foil, it is necessary to pack tightly；
If the 3, reagent is poured out from reagent bottle, it is impossible to reused.
2nd, main agents：
The reagent that the present embodiment is used includes extract solution, mobile phase and probe washing lotion, and the specific reagent in the present embodiment is matched somebody with somebody
Fang Wei：
(1) internal standard：The Isotopic Internal Standard and concentration of each vitamin provided from U.S.'s Cambridge Isotope Laboratories, it is as follows
Shown in table：
| Title | Commodity English name | Concentration |
| Vitamin B1 internal standard | Thiamine~d3Hydrochloride | 30nmol/L |
| Folic acid internal standard | Folic Acid~d2 | 4nmol/L |
| Vitamin A internal standard | Vitamin A~d5Acetate | 1.5μmol/L |
| The internal standard of 25(OH)VD 2 | 25~Hydroxyvitamin D2~[d6] | 10nmol/L |
| The internal standard of 25(OH)VD 3 | 25~Hydroxyvitamin D3~~[d6] | 30nmol/L |
| Vitamin E internal standard | α~Tocopherol~d6 | 6μg/mL |
| Vitamin K1 internal standard | Vitamin K1~[d7]) | 1.5ng/mL |
(2) extract solution：
Using one in formula as below：
Formula one：Include component according to percent by volume：84.75~98.95% methyl alcohol, 1~15% water and 0.05
~0.25% formic acid；
Formula two：Include component according to percent by volume：20~79% ethanol, 20~70% methyl alcohol and 1~10%
Water.
(3) mobile phase：
The selection of mobile phase has three kinds of formulas, can respectively with two kinds of extract recipe combined crosswises, below three kinds of differences match somebody with somebody
Side does not influence on the accuracy and precision of last testing result.
Formula one：Component including volumes below percentage：79.64~97.989% methyl alcohol, 2~20% water,
0.01~0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid, are well mixed
Closed ultrasonic deaerates 15~20 minutes at normal temperatures afterwards；
Formula two：Component including volumes below percentage：79.64~97.989% acetonitrile, 2~20% water,
0.01~0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid, are well mixed
Closed ultrasonic deaerates 15~20 minutes at normal temperatures afterwards；
Formula three：Component including volumes below percentage：49.71~84.989% methyl alcohol, 15~50% acetonitrile,
0.01~0.28% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid, are well mixed
Closed ultrasonic deaerates 15~20 minutes at normal temperatures afterwards.
(4) probe washing lotion：1st, 50~80% acetonitrile solution；2nd, 50~80% methanol aqueous solution.
(5) points for attention：
1st, the ratio of acetonitrile in the extract solution and mobile phase of publicity, water, formic acid, and methyl alcohol can be adjusted in respective range,
The adjustment of relevant parameter must be carried out on mass spectrograph simultaneously, so, the degree of accuracy of the vitamin content result for being detected and precision
Degree is unaffected；
2nd, all reagents select chromatogram rank；
3rd, the ultra-pure water of wet concentration tri-distilled water used or water purification machine filtering, its resistance >=18M Ω or conductance are configured<5us/
Cm, pH value is 7.0 ± 0.2；
4th, according to said extracted liquid and mobile phase formula in tandem mass spectrum instrument waters TQD models and AB3200 models
Go up parent ion, daughter ion and the Mass Spectrometry Conditions of each vitamin.
3rd, mass spectrometry parameters：
According to extract solution and mobile phase formula, the setting reference Mass Spectrometry Conditions on tandem mass spectrum instrument waters TQD
Such as following table:
Reference Mass Spectrometry Conditions according to the setting of extract solution and mobile phase formula on tandem mass spectrum instrument AB 3200 are as follows
Table：
It should be noted that parent ion is fixed in above-mentioned parameter, join for different machines or same brand machine mass spectrum
During number change, daughter ion is possible to change.
Wherein, liquid phase pump gradient and mass spectrograph condition such as following table：
It should be noted that because every machine is when debugging is installed, the difference such as precision of machine, above parameter is only supplied
With reference to specific tuning parameter does a little change by engineer.
Total ion figure obtained by method as the present embodiment as shown in Figure 1, abscissa represents the time, and ordinate is represented
Total ion abundance, the figure shows all scanning amount of ions for treating that measured ion is total, may determine that total ion abundance is by the figure
It is no to reach detection requirement, if be not reaching to detection required, influence can be brought on result accuracy.
The mass spectrogram of each composition after scanning obtained by the method by the present embodiment as shown in Figure 2, abscissa represents mother
The mass-to-charge ratio (monitoring number in real time, be not sized) of ion, ordinate represents ionic strength, the figure shows different detections
The amount of ions of material, vitamine concentration to be measured can be calculated by the figure.
Embodiment 2 detects multivitamin kit in dry blood spot simultaneously
According to the different reagent of the setting respectively proportionings and tandem mass spectrum condition of blood sample, the kit of this implementation can be once
Property measure 11 it is water-soluble and 7 in liposoluble vitamin, the vitamin that can be measured is as shown in the table：
| Water soluble vitamin | Liposoluble vitamin |
| Niacinamide usp | Vitamin A |
| Nicotinic acid | Calciferol |
| Vitamin B1 | Vitamine D3 |
| Vitamin B2 | 25-OH Vintamin D2 |
| Puridoxine hydrochloride | 25- hydroxyls Wiki life element D3 |
| Hydrochloric acid pyridoxamine | Vitamin E |
| Pyridoxal hydrochloride | Vitamin K1 |
| Folic acid | |
| Pantothenic acid | |
| Biotin | |
| Vitamin B12 |
The kit of the present embodiment includes：Internal standard mixed liquor, extract solution, mobile phase and probe washing lotion, in internal standard mixed liquor
Including internal standard product, the Isotopic Internal Standard of the various vitamins that internal standard product are provided using U.S.'s Cambridge Isotope Laboratories and its dense
Degree, extract solution and the mobile phase collocation that can intersect is used.
(1) use of internal standard mixed liquor：
The Isotopic Internal Standard and concentration of the various vitamins provided using U.S.'s Cambridge Isotope Laboratories, by various internal standards
High concentration internal standard mixed liquor is mixed to get, it is when in use, according to required ratio that high concentration internal standard mixed liquor is dilute with mobile phase
Release, and be fully uniformly mixed so as to obtain internal standard working solution.
(2) selection of mobile phase has three kinds of formulas, can respectively with two kinds of extract recipe combined crosswises, these three differences match somebody with somebody
Side does not influence on the accuracy and precision of last testing result, and mobile phase is using in formula as below：
Formula one：Component including volumes below percentage：79.64~97.989% methyl alcohol, 2~20% water,
0.01~0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid, are well mixed
Closed ultrasonic deaerates 15~20 minutes at normal temperatures afterwards；
Formula two：Component including volumes below percentage：79.64~97.989% acetonitrile, 2~20% water,
0.01~0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid, are well mixed
Closed ultrasonic deaerates 15~20 minutes at normal temperatures afterwards；
Formula three：Component including volumes below percentage：49.71~84.989% methyl alcohol, 15~50% acetonitrile,
0.01~0.28% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid, are well mixed
Closed ultrasonic deaerates 15~20 minutes at normal temperatures afterwards.
(3) extract solution is using in formula as below：
Formula one：Include component according to percent by volume：84.75~98.95% methyl alcohol, 1~15% water and 0.05
~0.25% formic acid；
Formula two：Include component according to percent by volume：20~79% ethanol, 20~70% methyl alcohol and 1~10%
Water.
(4) probe washing lotion：Following one of which can be used：
1.50~80% acetonitrile solution；2.50~80% methanol aqueous solution.
The method of embodiment 1 coordinates the domestic conventional tandem mass spectrometer (MS/ of American AB company and WATERS companies of the U.S.
MS analyzers) and embodiment 2 kit, various water soluble vitamins are given birth in simultaneously detecting dry blood spot by once experiment
Element and liposoluble vitamin, testing result have accuracy very high and the degree of accuracy.
It is last to should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent, without deviating from the essence of technical solution of the present invention
And scope.
Claims (9)
1. it is a kind of to detect multivitamin method in dry blood spot simultaneously, it is characterised in that to comprise the following steps：
(1) oscillation incubation after mixing dry blood spot sample and extract solution, is then centrifuged for, and takes supernatant；
(2) detected using tandem mass spectrometry after step (1) gained supernatant is mixed with mobile phase and internal standard mixed liquor, led to
The interior target ionic strength for comparing vitamin and vitamin is crossed come the content of vitamin in drawing test serum or test plasma.
2. method according to claim 1, it is characterised in that extract solution includes volumes below percentage in the step (1)
The component of ratio：84.75~98.95% methyl alcohol, 1~15% water and 0.05~0.25% formic acid；Or extract solution includes
The component of volumes below percentage：20~79% ethanol, 20~70% methyl alcohol and 1~10% water.
3. according to claim 1 and 2 at the same detect dry blood spot in multivitamin method, it is characterised in that
The acquisition method of dry blood spot sample is in the step (1)：Fresh whole blood is dripped on quantitative filter paper piece, drop of blood is distributed
Uniformly, to dry rear sealing preserve standby for filter paper.
4. according to claim 1 and 2 at the same detect dry blood spot in multivitamin method, it is characterised in that
Chromatographic condition in the step (2)：
Mobile phase includes the component of volumes below percentage：79.64~97.989% methyl alcohol, 2~20% water, 0.01~
0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid；Or mobile phase includes
The component of volumes below percentage：79.64~97.989% acetonitrile, 2~20% water, 0.01~0.35% formic acid, with
And 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid at least one；Or mobile phase includes volumes below percentage
Component：49.71~84.989% methyl alcohol, 15~50% acetonitrile, 0.01~0.28% formic acid, and 0.001~
At least one in 0.01% perfluorobutyric acid and trifluoroacetic acid；
Probe washing lotion is the acetonitrile solution that volumetric concentration is 50~80%, or probe washing lotion is that volumetric concentration is 50~80%
Methanol aqueous solution.
5. it is a kind of to detect multivitamin kit in dry blood spot simultaneously, it is characterised in that including：Internal standard mixed liquor,
Extract solution, mobile phase and probe washing lotion.
6. according to claim 5 at the same detect dry blood spot in multivitamin kit, it is characterised in that institute
State component of the extract solution including volumes below percentage：84.75~98.95% methyl alcohol, 1~15% water and 0.05~
0.25% formic acid；Or extract solution includes the component of volumes below percentage：20~79% ethanol, 20~70% methyl alcohol
Water with 1~10%.
7. according to claim 5 at the same detect dry blood spot in multivitamin kit, it is characterised in that institute
State component of the mobile phase including volumes below percentage：79.64~97.989% methyl alcohol, 2~20% water, 0.01~
0.35% formic acid, and at least one in 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid；Or mobile phase includes
The component of volumes below percentage：79.64~97.989% acetonitrile, 2~20% water, 0.01~0.35% formic acid, with
And 0.001~0.01% perfluorobutyric acid and trifluoroacetic acid at least one；Or mobile phase includes volumes below percentage
Component：49.71~84.989% methyl alcohol, 15~50% acetonitrile, 0.01~0.28% formic acid, and 0.001~
At least one in 0.01% perfluorobutyric acid and trifluoroacetic acid.
8. according to claim 5 at the same detect dry blood spot in multivitamin kit, it is characterised in that institute
It is the acetonitrile solution that volumetric concentration is 50~80% to state probe washing lotion, or probe washing lotion is that volumetric concentration is 50~80%
Methanol aqueous solution.
9. according to claim 5 at the same detect dry blood spot in multivitamin kit, it is characterised in that institute
Stating internal standard mixed liquor includes vitamin B1 internal standard, folic acid internal standard, vitamin A internal standard, the internal standard of 25(OH)VD 2,25 hydroxyls dimension
Raw element D3 internal standards, vitamin E internal standard and vitamin K1 internal standard.
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| CN201710100120.2A CN106770802B (en) | 2017-02-23 | 2017-02-23 | Method and kit for simultaneously detecting multiple vitamins in dry blood filter paper sheet |
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| CN201710100120.2A CN106770802B (en) | 2017-02-23 | 2017-02-23 | Method and kit for simultaneously detecting multiple vitamins in dry blood filter paper sheet |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108195984A (en) * | 2017-12-29 | 2018-06-22 | 汤臣倍健股份有限公司 | Multivitamin detection method and detecting system in a kind of dry blood cake |
| CN109001329A (en) * | 2018-08-21 | 2018-12-14 | 杭州凯莱谱精准医疗检测技术有限公司 | The high performance liquid chromatography tandem mass spectrum detection method of 25-hydroxy-vitamin D in dried blood spot |
| US10656059B2 (en) | 2018-03-07 | 2020-05-19 | Alcala Pharmaceutical, Inc. | Method for qualitative and quantitative multiplexing of drug analytes from biological samples |
| CN113390976A (en) * | 2021-03-04 | 2021-09-14 | 杭州凯莱谱精准医疗检测技术有限公司 | Detection kit for detecting fat-soluble vitamins in serum by high performance liquid chromatography tandem mass spectrometry and detection method thereof |
| CN115308346A (en) * | 2022-09-02 | 2022-11-08 | 汤臣倍健股份有限公司 | Method for extracting and detecting content of multiple vitamins in blood |
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| CN113390976A (en) * | 2021-03-04 | 2021-09-14 | 杭州凯莱谱精准医疗检测技术有限公司 | Detection kit for detecting fat-soluble vitamins in serum by high performance liquid chromatography tandem mass spectrometry and detection method thereof |
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