CN106770085B - A kind of ballast water for ship device for fast detecting and method based on micro-fluidic chip - Google Patents
A kind of ballast water for ship device for fast detecting and method based on micro-fluidic chip Download PDFInfo
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Abstract
The invention discloses a kind of ballast water for ship device for fast detecting and detection method based on micro-fluidic chip, described device includes platform, separation assembly, sample pre-treatments component, light excitation component, photodetection assembly and data handling component, the separation assembly is bonded by multi-layer micro-fluidic chips, is sufficiently bonded between each layer micro-fluidic chip.Different microtrabeculae gaps is arranged in different layers for the size of agent particle size by three layers or multi-layer micro-fluidic chips by the present invention, can quickly be separated by three layers or multi-layer micro-fluidic chips.There is detection zone at every layer, the substance separated can be detected in time, realizes and the substance in sample is quickly separated and detection.Sample is put into sample pre-treatments component after sample pre-treatments component carries out coarse filtration and the substance of larger size is filtered and purified, to prevent the blocking of microchannel by the present invention.
Description
Technical field
The present invention relates to the detection technique of microalgae in ballast water for ship, especially a kind of ship pressure based on micro-fluidic chip
Carry water device for fast detecting and method.
Background technique
Ballast water for ship is one of main harm of marine biological invasion, and external marine biological invasion will lead to local
The ecosystem is destroyed, and mainly includes agricultural and causing damages to the health of the mankind to industry.In " ballast water for ship
And sediment monitoring and management international convention " in define ship discharge when ballast water performance should meet standard.It is wherein important
One be exactly the biological sizes of difference survived in ballast water for ship concentration, however microalgae cell be in ballast water for ship most often
The biology seen, and one of the main target that should be handled and detect now.
Currently, flow cytometer (flow cytometry, FCM) be to high-speed straight-line flowing cell or biological particle into
The instrument of row quantitative determination and analysis is also applied for the detection and analysis of fluorescent samples, and main includes the flowing skill of sample
Art, the counting of cell and sorting technology, computer is to the acquisition of data and analytical technology etc..Flow cytometer is with flow cytometry
It is the subjects such as hydrodynamics, laser technology, electronic engineering, molecular immunology, cell fluorescence and computer for theoretical basis
The crystallization of knowledge integration application.Its main feature is that: measuring speed is fast, tested group is big, can carry out measuring multiple parameters, in biology, doctor
Field is widely used.But the equipment instrument is huge, expensive, cannot integrate portable, is also unfavorable for being widely applied.
For above problem, there has been proposed the portable flow cytometer based on micro-fluidic chip, micro-fluidic chips
The units such as pretreatment, reaction, separation and detection are integrated into one single chip by (microfluidic chip) technology, have collection
The features such as high, small in size at degree, represents the direction of micromation Instrument Development.
Disclosed in Chinese patent 201310491678.X " a kind of Portable fluorescence detection device based on micro-fluidic chip and
Its detection method " and " a kind of analytical equipment of ballast for cruising water treatment procedure and its analysis side disclosed in 201410249765.9
Method " belong to the portable flow cytometer based on micro-fluidic chip.
But above-mentioned patent is all based on single layer micro-fluidic chip technology, single layer micro-fluidic chip to microalgae cell or
The separation detection of particle can achieve the effect that it is limited, especially to various sizes of cell or particle on single layer micro-fluidic chip
When separate, due to the size of particle or cell difference, the microchannel on single layer micro-fluidic chip is easy to by big
Particle or cell are blocked, and micro-fluidic chip cisco unity malfunction is caused, to influence to the detection of ballast water for ship as a result, removing
Except this, when the substance complexity contained in the sample, the effect of single layer micro-fluidic chip is greatly limited, and is difficult to meet and is reached
To separating and detect integrated demand.
Summary of the invention
To solve the above problems existing in the prior art, the present invention, which will design one kind, can prevent micro-fluidic chip microchannel
It blocks and is able to achieve one kind for on-site rapid measurement that point analysis of variance is integrated, integrated level is high, label-free and be based on
The ballast water for ship device for fast detecting and method of micro-fluidic chip.
To achieve the goals above, technical scheme is as follows: a kind of ballast water for ship based on micro-fluidic chip
Device for fast detecting, including platform, separation assembly, sample pre-treatments component, light excitation component, photodetection assembly and data processing
Component, the platform are darkroom structure, and separation assembly and photodetection assembly be fixed in platform, the separation assembly respectively with sample
Product pre-treatment component, photodetection assembly are connected with light excitation component, and the photodetection assembly is connect with data handling component；
The sample pre-treatments component includes strainer, and for the pre-treatment and purification of sample, treated sample is logical
It crosses micro-pipe and enters separation assembly；
The separation assembly is bonded by multi-layer micro-fluidic chips, is sufficiently bonded between each layer micro-fluidic chip；First
Sample cell, sheath liquid bath, mixed liquor channel, separating tank, sense channel and waste liquid reservoir are set on layer micro-fluidic chip, it is intermediate each
Cubic array area, separating tank, sense channel and waste liquid reservoir, most the lower layer of micro-fluidic chip is arranged in floor micro-fluidic chip
Cubic array area, sense channel and waste liquid reservoir, and the upper layer cubic array area of adjacent two layers micro-fluidic chip are set
Microtrabeculae interval be greater than lower layer's cubic array area microtrabeculae interval.
Separation assembly of the present invention is sufficiently bonded by three layers of micro-fluidic chip；It is arranged on first layer micro-fluidic chip
Sample cell, sheath liquid bath A, sheath liquid bath B, mixed liquor channel, separating tank I, sense channel I and waste liquid reservoir I, the sample cell,
Sheath liquid bath A and sheath liquid bath B passes through sample channel, sheath fluid channel A and sheath fluid channel B respectively and is connected to mixed liquor channel, described mixed
It closes liquid channel to be connected to through separating tank I with sense channel I, the end of sense channel I connects waste liquid reservoir I；The sample cell, sheath
Liquid bath A and sheath liquid bath B is equipped with liquid injection pump, plays the role of pushing liquid；
Cubic array area I, separating tank II, sense channel II and waste liquid reservoir are set on second layer micro-fluidic chip
II, I entrance of cubic array area is connected to the separating tank I of first layer micro-fluidic chip, the outlet of cubic array area I is through dividing
It is connected to from slot II with sense channel II, the end of sense channel II connects waste liquid reservoir II；The cubic array area I it is micro-
Intercolumniation is divided into 18-22 μm；
Cubic array area II, sense channel III and waste liquid reservoir III are set on third layer micro-fluidic chip, it is described vertical
II entrance of cube array area is connected to the separating tank II of second layer micro-fluidic chip, the outlet of cubic array area II is through sense channel
III connection waste liquid reservoir III；9-11 μm is divided between the microtrabeculae in the cubic array area II.
Sample cell, sheath liquid bath A and sheath liquid bath B of the present invention pass through respective channel is connected to mixed liquor channel one
End.
Sample pre-treatments component of the present invention includes strainer, and it includes microprocessor that the data handling component, which uses,；Institute
Stating photodetection assembly includes photomultiplier tube or single photon counting module.
At the top of platform, photodetection assembly is internally located at point sample pre-treatments component package of the present invention platform
Lower section from component, data handling component platform outside and be encapsulated in the bottom of platform, light excitation component is in the side of platform
It is connect on wall and by sealing structure with platform.
Each layer of micro-fluidic chip of separation assembly of the present invention has microchannel, first layer micro-fluidic chip microchannel
Opening downwards, the opening of second layer micro-fluidic chip microchannel faces upward, the opening of third layer micro-fluidic chip microchannel
Upwardly；First layer micro-fluidic chip microchannel includes sample channel, sheath fluid channel A, sheath fluid channel B, mixed liquor channel and inspection
Survey channel I；Second layer micro-fluidic chip microchannel includes the company between sense channel II, cubic array area I and separating tank II
Connect road；Third layer micro-fluidic chip microchannel includes sense channel III.
A kind of detection method of the ballast water for ship device for fast detecting based on micro-fluidic chip, includes the following steps:
A, biggish substance will be differed with the dimensions of detection substance by sample solution being put into sample pre-treatments component
Fall with contaminant filter, the sample handled enters the sample in separation assembly by the micro-pipe that pre-treatment component is connected with separation assembly
Product slot；
Sheath fluid is added to sheath liquid bath A and sheath liquid bath B, under the impetus of liquid injection pump, makes sheath fluid and pre-treatment
Sample respectively along sheath fluid channel A, sheath fluid channel B and sample channel pass through first layer micro-fluidic chip separating tank I, utilize
The filtration in cubic array area I is separated, dimensions be greater than cubic array area I microtrabeculae interval cell with it is micro-
Grain enters sense channel I, carries out the detection of activity and quantity, then flows into waste liquid reservoir I；Similarly second layer micro-fluidic chip
Carry out cell of the dimensions between the microtrabeculae interval in cubic array area II and the microtrabeculae interval in cubic array area I with it is micro-
The separation and the detection of activity and quantity of grain；Third layer micro-fluidic chip carries out dimensions in the microtrabeculae in cubic array area II
It is spaced the separation of cell and particle below and carries out the detection of activity and quantity.
B, when have sample enter any one separating tank when, open light excitation component, photodetection assembly, data handling component,
The liquid injection of sheath liquid bath pumps, and making sheath fluid and microalgae respectively along sheath fluid channel and sample channel, channel flows to waste liquid after testing
Reservoir, microalgae after testing region when the light excitation that is excited of the unicellular inner chlorophyll of microalgae generate instantaneous photon radiation；
C, by the power of pulse signal and quantity, activity of microalgae power and microalgae quantity in ballast water for ship are obtained.
Compared with prior art, the invention has the following beneficial effects:
1, due to the difference of the agent particle size size in sample, the present invention passes through three layers or multi-layer micro-fluidic chips, for
Different microtrabeculae gaps is arranged in different layers for the size of agent particle size, can be according to the of different sizes of agent particle size to be separated
It is designed, microtrabeculae gap can be designed to that 3 μm or more of arbitrary dimension, structure flexible design can quickly meet different
Actual needs, for various sizes of substance, can quickly be separated by three layers or multi-layer micro-fluidic chips.Every
Layer has detection zone, can be detected in time to the substance separated, realize and quickly separate to the substance in sample
With detection.
2, operation of the present invention is simple, and sample need to be only put into sample pre-treatments component, and sample pre-treatments component carries out thick mistake
After filter, into separation assembly, liquid injection pump and other corresponding components are opened, separation assembly can automatically separate and detection group
Part can be detected timely, without human interference, this operation without professional, meet the needs of on-site test.
3, sample is put into sample pre-treatments component by the present invention, after sample pre-treatments component carries out coarse filtration, by larger ruler
Very little substance is filtered and has been purified, to prevent the blocking of microchannel.
4, ballast water for ship of the invention is under sheath fluid effect, and microalgae cell focuses the latter one warp in ballast water for ship
Detection zone is crossed, the error for avoiding multiple microalgae cells while after testing region and generating utilizes one at detection zone
Beam exciting light irradiates microalgae cell, so that microalgae cell inner chlorophyll is absorbed exciting light and generates chlorophyll fluorescence, chlorophyll fluorescence
It is detected by photodetection assembly, light intensity magnitude characterizes microalgae cell activity power, and light intensity is stronger, then microalgae cell activity is stronger；
Conversely, light intensity is weaker, then microalgae cell activity is weaker.The present invention is automatically performed pair by detecting microalgae intrinsic fluorescence signal intensity
The differentiation of activity of microalgae, it is easy to operate, when overcoming artificial counting in conventional method bring human factor influence, error and
Must have the requirement that aquatile abundant is gained knowledge to operator.
5, the present invention for the differentiation of activity of microalgae is carried out by detecting microalgae autofluorescence information, these fluorescence letter
Breath is the inherent probe closely related with its activity, in this way without carrying out external label to microalgae, can not only overcome external mark
Bring error during note, it is complicated for operation, time-consuming the disadvantages of, it is often more important that it is this to exempt from using in microalgae itself in probe
Labeling method has universality；
6, the microfluidic platform of the invention due to being detected using multi-layer micro-fluidic chips as ballast water for ship, and relevant photoelectricity
The structure type of small volume also can be used in detection device, and accordingly, with respect to existing large-scale detection device, this detection device has
It is small in size, light-weight, easy to carry, it is able to carry out the advantages that holding for on-site test.
7, platform of the invention is darkroom structure, can effectively exclude the interference of remaining stray light in addition to exciting light, and light swashs
Hair component is preferably fixed in platform or is connect by sealing structure with platform, and its object is to keep platform opaque.
Detailed description of the invention
The present invention shares 5 width of attached drawing, in which:
Fig. 1 is device for fast detecting structural schematic diagram in ballast water for ship.
Overall schematic after the three layers of micro-fluidic chip bonding of the position Fig. 2 separation assembly.
Fig. 3 is the structural schematic diagram of first layer micro-fluidic chip in separation assembly.
Fig. 4 is the structural schematic diagram of second layer micro-fluidic chip in separation assembly.
Fig. 5 is the structural schematic diagram of third layer micro-fluidic chip in separation assembly.
Wherein: 1, sample pre-treatments component, 2, platform, 3, separation assembly, 4, light excitation component, 5, photodetection assembly, 6,
Data handling component, 7, sample cell, 8, sheath liquid bath A, 9, sheath liquid bath B, 10, mixed liquor channel, 11, separating tank I, 12, detection it is logical
Road I, 13, waste liquid reservoir I, 14, cubic array area I, 15, separating tank II, 16, sense channel II, 17, waste liquid reservoir
II, 18, cubic array area II, 19, sense channel III, 20, waste liquid reservoir III.
Specific embodiment
Following further describes the present invention with reference to the drawings；Following non-limiting embodiments can make the general of this field
The present invention, but do not limit the invention in any way is more fully understood in logical technical staff.
As shown in Figs. 1-5, a kind of ballast water for ship device for fast detecting based on micro-fluidic chip, including platform 2, separation
Component 3, sample pre-treatments component 1, light excitation component 4, photodetection assembly 5 and data handling component 6, the platform 2 are darkroom
Structure, separation assembly 3 and photodetection assembly 5 are fixed in platform 2, the separation assembly 3 respectively with sample pre-treatments component 1,
Photodetection assembly 5 and light excitation component 4 connect, and the photodetection assembly 5 is connect with data handling component 6；
The sample pre-treatments component 7 includes strainer, and for the pre-treatment and purification of sample, treated sample is logical
It crosses micro-pipe and enters separation assembly 3；
The separation assembly 3 is bonded by multi-layer micro-fluidic chips, is sufficiently bonded between each layer micro-fluidic chip；First
Sample cell, sheath liquid bath, mixed liquor channel, separating tank, sense channel and waste liquid reservoir are set on layer micro-fluidic chip, it is intermediate each
Cubic array area, separating tank, sense channel and waste liquid reservoir, most the lower layer of micro-fluidic chip is arranged in floor micro-fluidic chip
Cubic array area, sense channel and waste liquid reservoir, and the upper layer cubic array area of adjacent two layers micro-fluidic chip are set
Microtrabeculae interval be greater than lower layer's cubic array area microtrabeculae interval.
Separation assembly 3 of the present invention is sufficiently bonded by three layers of micro-fluidic chip；It is set on first layer micro-fluidic chip
Set sample cell 7, sheath liquid bath A8, sheath liquid bath B9, mixed liquor channel 10, separating tank I 11, sense channel I 12 and waste liquid reservoir I
13, the sample cell 7, sheath liquid bath A8 and sheath liquid bath B9 pass through respectively sample channel, sheath fluid channel A and sheath fluid channel B with mix
Liquid channel 10 is connected to, and the mixed liquor channel 10 is connected to through separating tank I 11 with sense channel I 12, and the end of sense channel I 12 connects
Connect waste liquid reservoir I 13；The sample cell 7, sheath liquid bath A8 and sheath liquid bath B9 are equipped with liquid injection pump, play and carry out to liquid
The effect of promotion；
Cubic array area I 14, separating tank II 15, sense channel II 16 and waste liquid storage are set on second layer micro-fluidic chip
Liquid bath II 17, I 14 entrance of cubic array area is connected to the separating tank I 11 of first layer micro-fluidic chip, cubic array
The outlet of area I 14 is connected to through separating tank II 15 with sense channel II 16, and the end of sense channel II 16 connects waste liquid reservoir II 17；
18-22 μm is divided between the microtrabeculae in the cubic array area I 14；
Cubic array area II 18, sense channel III 19 and waste liquid reservoir III 20 are set on third layer micro-fluidic chip,
II 18 entrance of cubic array area is connected to the separating tank II 15 of second layer micro-fluidic chip, cubic array area II 18 goes out
Mouth connects waste liquid reservoir III 20 through sense channel III 19；9-11 μm is divided between the microtrabeculae in the cubic array area II 18.
Sample cell 7, sheath liquid bath A8 and sheath liquid bath B9 of the present invention pass through respective channel and are connected to mixed liquor channel
10 one end.
Sample pre-treatments component 1 of the present invention includes strainer, and the data handling component 6 is used including microprocessor；
The photodetection assembly 5 includes photomultiplier tube or single photon counting module.
Sample pre-treatments component 1 of the present invention is encapsulated in the top of platform 2, and photodetection assembly 5 is at the interior position of platform 2
In the lower section of separation assembly 3, data handling component 6 platform 2 outside and be encapsulated in the bottom of platform 2, light excitation component 4 exists
It is connect on the side wall of platform 2 and by sealing structure with platform 2.
Each layer of micro-fluidic chip of separation assembly 3 of the present invention has microchannel, and first layer micro-fluidic chip is micro- logical
Downwards, the opening of second layer micro-fluidic chip microchannel faces upward the opening in road, and third layer micro-fluidic chip microchannel is opened
Mouth faces upward；First layer micro-fluidic chip microchannel includes sample channel, sheath fluid channel A, sheath fluid channel B, mixed liquor channel 10
With sense channel I 12；Second layer micro-fluidic chip microchannel includes sense channel II 16, cubic array area I 14 and separating tank
Interface channel between II 15；Third layer micro-fluidic chip microchannel includes sense channel III 19.
A kind of detection method of the ballast water for ship device for fast detecting based on micro-fluidic chip, includes the following steps:
A, biggish substance will be differed with the dimensions of detection substance by sample solution being put into sample pre-treatments component 1
Fall with contaminant filter, the sample handled enters in separation assembly 3 by the micro-pipe that pre-treatment component is connected with separation assembly 3
Sample cell 7；
Sheath fluid is added to sheath liquid bath A8 and sheath liquid bath B9, under the impetus of liquid injection pump, makes sheath fluid and preceding place
The sample of reason passes through the separating tank I 11 of first layer micro-fluidic chip respectively along sheath fluid channel A, sheath fluid channel B and sample channel,
It is separated using the filtration in cubic array area I 14, dimensions is greater than the microtrabeculae interval in cubic array area I 14
Cell and particle enter sense channel I 12, carry out the detection of activity and quantity, then flow into waste liquid reservoir I 13；Similarly second
Floor micro-fluidic chip carries out dimensions between the microtrabeculae interval in cubic array area II 18 and the microtrabeculae in cubic array area I 14
The separation and the detection of activity and quantity of cell and particle between；Third layer micro-fluidic chip carry out dimensions cube
The separation of the microtrabeculae interval cell below and particle in volume array area II 18 and the detection for carrying out activity and quantity.
B, when there is sample to enter any one separating tank, light excitation component 4, photodetection assembly 5, data handling component are opened
6, the liquid injection pump of sheath liquid bath, make sheath fluid and microalgae respectively along sheath fluid channel and sample channel after testing channel flow to it is useless
Liquid reservoir, microalgae after testing region when the light excitation that is excited of the unicellular inner chlorophyll of microalgae generate instantaneous photon radiation；
C, by the power of pulse signal and quantity, activity of microalgae power and microalgae quantity in ballast water for ship are obtained.
One embodiment of the present of invention is as follows:
Detection method of the invention, includes the following steps:
1,5 μ L ballast for cruising water samples are put into sample pre-treatments component 1 and carry out coarse filtration and purification, the sample handled
Product can enter the sample cell 7 in separation assembly 3 in the micro-pipe that sample pre-treatments component 7 is connected with separation assembly 3, by 10 μ L sheaths
Liquid is added separately to sheath liquid bath A8 and sheath liquid bath B9, opens light excitation component 4, photodetection assembly 5, data handling component 6, opens
The liquid injection of sheath liquid bath sheath fluid slot A8, sheath liquid bath B9 and sample cell 7 pump makes sheath fluid and the complete sample of pre-treatment respectively along mixed
It closes liquid channel 10 to be separated into separating tank I 11, the substance after separation enters I 12 pairs of substances of sense channel and detects, together
Reason, the substance after remaining two layers micro-fluidic chip separation enter sense channel II 16 and sense channel III 19, mixed liquor pass through with
The separating tank of upper three layers of micro-fluidic chip, complete separation, then the substance of the different dimensions of each layer of micro-fluidic chip into
Enter the detection zone of each layer.
2, due in ballast water for ship microalgae after testing region when the unicellular inner chlorophyll of microalgae be excited light excitation produce
Raw instantaneous photon radiation；So will be optically excited component 4 by the microalgae of each layer detection zone excites optical signals.
3, the optical signal generated by excitation that each microalgae issues is received using photodetection assembly 5.
4, using data handling component 6, digital pulse signal is converted optical signal into, can be determined by the number of pulse
The density of the microalgae of microalgae in the sample.
It is completed by above step and quickly separation and detection is carried out to the microalgae and particle of different dimensions.
Claims (6)
1. a kind of ballast water for ship device for fast detecting based on micro-fluidic chip, including platform (2), separation assembly (3), sample
Pre-treatment component (1), light excitation component (4), photodetection assembly (5) and data handling component (6), the platform (2) are darkroom
Structure, separation assembly (3) and photodetection assembly (5) are fixed in platform (2), and the separation assembly (3) is respectively and before sample
It manages component (1), photodetection assembly (5) and light excitation component (4) to connect, the photodetection assembly (5) and data handling component (6)
Connection；
The sample pre-treatments component (1) includes strainer, and for the pre-treatment and purification of sample, treated sample passes through
Micro-pipe enters separation assembly (3)；
The separation assembly (3) is bonded by multi-layer micro-fluidic chips, is sufficiently bonded between each layer micro-fluidic chip；First layer
Sample cell, sheath liquid bath, mixed liquor channel, separating tank, sense channel and waste liquid reservoir, intermediate each layer are set on micro-fluidic chip
Micro-fluidic chip setting cubic array area, separating tank, sense channel and waste liquid reservoir, most the lower layer of micro-fluidic chip are set
Set cubic array area, sense channel and waste liquid reservoir, and the upper layer cubic array area of adjacent two layers micro-fluidic chip
Microtrabeculae interval is greater than the microtrabeculae interval in lower layer's cubic array area；
It is characterized by: the separation assembly (3) is sufficiently bonded by three layers of micro-fluidic chip；On first layer micro-fluidic chip
Sample cell (7), sheath liquid bath A (8), sheath liquid bath B (9), mixed liquor channel (10), separating tank I (11), sense channel I (12) are set
With waste liquid reservoir I (13), the sample cell (7), sheath liquid bath A (8) and sheath liquid bath B (9) are logical by sample channel, sheath fluid respectively
Road A and sheath fluid channel B is connected to mixed liquor channel (10), and the mixed liquor channel (10) is through separating tank I (11) and sense channel I
(12) it is connected to, the end of sense channel I (12) connects waste liquid reservoir I (13)；The sample cell (7), sheath liquid bath A (8) and sheath
Liquid bath B (9) is equipped with liquid injection pump, plays the role of pushing liquid；
Cubic array area I (14), separating tank II (15), sense channel II (16) and waste liquid are set on second layer micro-fluidic chip
Reservoir II (17), cubic array area I (14) entrance are connected to the separating tank I (11) of first layer micro-fluidic chip, are vertical
Cube array area I (14) outlet is connected to through separating tank II (15) with sense channel II (16), and the end of sense channel II (16) connects
Connect waste liquid reservoir II (17)；18-22 μm is divided between the microtrabeculae of the cubic array area I (14)；
Cubic array area II (18), sense channel III (19) and waste liquid reservoir III are set on third layer micro-fluidic chip
(20), cubic array area II (18) entrance be connected to the separating tank II (15) of second layer micro-fluidic chip, cube battle array
It arranges area II (18) outlet and connects waste liquid reservoir III (20) through sense channel III (19)；The cubic array area II (18) it is micro-
Intercolumniation is divided into 9-11 μm.
2. a kind of ballast water for ship device for fast detecting based on micro-fluidic chip according to claim 1, it is characterised in that:
The sample cell (7), sheath liquid bath A (8) and sheath liquid bath B (9) are connected to the one of mixed liquor channel (10) by respective channel
End.
3. a kind of ballast water for ship device for fast detecting based on micro-fluidic chip according to claim 1, it is characterised in that:
The sample pre-treatments component (1) includes strainer, and the data handling component (6) uses including microprocessor；The light detection
Component (5) includes photomultiplier tube or single photon counting module.
4. a kind of ballast water for ship device for fast detecting based on micro-fluidic chip according to claim 1, it is characterised in that:
The sample pre-treatments component (1) is encapsulated in the top of platform (2), and photodetection assembly (5) is internally located at separation platform (2)
The lower section of component (3), data handling component (6) platform (2) outside and be encapsulated in the bottoms of platform (2), light excitation component
(4) it is connect on the side wall of platform (2) and through sealing structure with platform (2).
5. a kind of ballast water for ship device for fast detecting based on micro-fluidic chip according to claim 2, it is characterised in that:
Each layer of micro-fluidic chip of the separation assembly (3) has a microchannel, the opening of first layer micro-fluidic chip microchannel towards
Under, the opening of second layer micro-fluidic chip microchannel faces upward, and the opening of third layer micro-fluidic chip microchannel faces upward；First
Layer micro-fluidic chip microchannel includes sample channel, sheath fluid channel A, sheath fluid channel B, mixed liquor channel (10) and sense channel I
(12)；Second layer micro-fluidic chip microchannel includes sense channel II (16), cubic array area I (14) and separating tank II (15)
Between interface channel；Third layer micro-fluidic chip microchannel includes sense channel III (19).
6. a kind of detection method of the ballast water for ship device for fast detecting based on micro-fluidic chip, it is characterised in that: including such as
Lower step:
A, sample solution is put into sample pre-treatments component (1) will differ with the dimensions of detection substance biggish substance and
Contaminant filter falls, and the sample handled enters in separation assembly (3) by the micro-pipe that pre-treatment component is connected with separation assembly (3)
Sample cell (7)；
Sheath fluid is added to sheath liquid bath A (8) and sheath liquid bath B (9), under the impetus of liquid injection pump, makes sheath fluid and preceding place
The sample of reason passes through the separating tank I of first layer micro-fluidic chip respectively along sheath fluid channel A, sheath fluid channel B and sample channel
(11), it is separated using the filtration in cubic array area I (14), dimensions is greater than cubic array area I (14)
The cell and particle at microtrabeculae interval enter sense channel I (12), carry out the detection of activity and quantity, then flow into waste liquid reservoir
Ⅰ(13)；Similarly second layer micro-fluidic chip carries out dimensions in the microtrabeculae interval in cubic array area II (18) and cube
The separation and the detection of activity and quantity of cell and particle between the microtrabeculae interval of array area I (14)；The micro-fluidic core of third layer
Piece carries out dimensions in the separation of the microtrabeculae interval cell below and particle in cubic array area II (18) and carries out activity
With the detection of quantity；
B, when there is sample to enter any one separating tank, light excitation component (4), photodetection assembly (5), data handling component are opened
(6), the liquid injection pump of sheath liquid bath, making sheath fluid and microalgae respectively along sheath fluid channel and sample channel, channel flows to after testing
Waste liquid reservoir, microalgae after testing region when the light excitation that is excited of the unicellular inner chlorophyll of microalgae generate instantaneous photon radiation；
C, by the power of pulse signal and quantity, activity of microalgae power and microalgae quantity in ballast water for ship are obtained.
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| CN107271630A (en) * | 2017-07-13 | 2017-10-20 | 大连海事大学 | A kind of ballast hydroplankton on-line measuring device and method |
| CN108645778A (en) * | 2018-05-24 | 2018-10-12 | 广西民族大学 | A kind of portable CD+4T lymphocytes detecting system and its automated cell counting algorithm |
| CN109030839A (en) * | 2018-05-24 | 2018-12-18 | 广西民族大学 | A kind of light fluid attachment based on smart phone |
| CN112337514A (en) * | 2019-08-09 | 2021-02-09 | 上海仁敬生物科技有限公司 | Micro-fluidic chip |
| CN112697679A (en) * | 2020-12-04 | 2021-04-23 | 杭州娃哈哈精密机械有限公司 | Device for rapidly detecting number of bacteria in beverage |
| WO2024007489A1 (en) * | 2022-07-05 | 2024-01-11 | 中科合肥智慧农业协同创新研究院 | On-site rapid detection device for soil organic matter, and detection method thereof |
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