CN106755228A - The technique for preparing the oligomeric Gly-His-Lys of selenium yeast using Se-enriched yeast - Google Patents
The technique for preparing the oligomeric Gly-His-Lys of selenium yeast using Se-enriched yeast Download PDFInfo
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Abstract
A kind of utilization Se-enriched yeast prepares the technique of the oligomeric Gly-His-Lys of selenium yeast, and step is as follows：1) wine brewing ferment is carried out into shaking table culture, recycles first class seed pot, secondary seed tank and three-level seeding tank Amplification Culture step by step, the culture feeding fermentation tank for obtaining adds sodium selenite to carry out fermented and cultured, and Se-enriched yeast is obtained through centrifuge washing；2) Se-enriched yeast is digested using protease, concentration of substrate is 12%, obtains Se-enriched yeast enzymolysis liquid；3) by Se-enriched yeast enzymolysis liquid in 53 55 DEG C of temperature, self-dissolving 2 hours under natural ph；4) pH value of the Se-enriched yeast enzymolysis liquid after self-dissolving is adjusted to 67, adds composite biological enzyme preparation, obtain enzymolysis liquid；5) enzymolysis liquid is hydrolyzed 20 24 hours at 55 ± 2 DEG C of temperature, enzymolysis liquid is heated to 85 DEG C 90 DEG C, ultrafiltration is carried out afterwards, obtain polypeptide filtrate；6) polypeptide filtrate is carried out into nanofiltration desalination, obtains desalination peptide liquid；7) desalination peptide liquid concentrated, dried, you can obtained the oligomeric Gly-His-Lys of selenium yeast.
Description
Technical field
The present invention relates to technical field of bioengineering, and in particular to one kind prepares the oligomeric Gly-His-Lys of selenium yeast using Se-enriched yeast
Technique.
Background technology
Protein is the material base of life, is the important substance of human life activity, and each tissue in body is thin
Born of the same parents and all important physiological activities, have protein to participate in, and do not have protein just without life.
The large biological molecule that protein is made up of one or more polypeptide chain, each polypeptide chain contains several to hundreds of
Individual amino acid residue, peptide chain rearranges the protein of various difference in functionalitys in a certain order.
Albumen oligopeptide (containing 3-9 amino acid molecular composition) be protein through the effect of the biological enzyme hydrolysis such as protease after,
The protein hydrolysate for obtaining is processed by special process again, a small amount of amino acid, the dimension life of B races are also contained in hydrolysate
The active ingredient such as element, nucleotides and inorganic salts, good water solubility, with good taste and be extremely easy to human consumption and absorb wait spy
Property；Albumen oligopeptide has following biological function：1st, strengthen human muscle's vigor, accelerate myoglobins to recover.Because peptide is easy
In absorption, can rapidly utilize, it is suppressed that the process of internal " negative nitrogen balance ", slow down the decomposition of myogen, normal egg in acceleration bodies
The synthesis of white matter, safeguards physiological function, reaches antifatigue effect.2nd, energetic supersession is promoted, albumen oligopeptide is than protein more
Energetic supersession can be promoted.3rd, human serum cholesterol is reduced, albumen oligopeptide can more hinder enteron aisle inner cholesterol than protein
Absorb, and accelerate it to excrete.
Selenium is the essential trace elements of the human body, is the important composition composition of internal various selenoproteins and selenoenzyme, tool
It is improved body immunity, anti-oxidant, anticancer, anti-AIDS, maintenance normal reproduction, the physiological function of prevention Kaschin-Beck disease.
And have not yet seen in the prior art using Se-enriched yeast and prepare the technical scheme of the oligomeric Gly-His-Lys of selenium yeast, in view of
This, proposes the application.
The content of the invention
The technical problems to be solved by the invention are to improve a kind of method to be easily achieved, good in economic efficiency utilization selenium-rich
The technique that yeast prepares the oligomeric Gly-His-Lys of selenium yeast.
The technical problems to be solved by the invention are realized using following technical scheme：
A kind of utilization Se-enriched yeast prepares the technique of the oligomeric Gly-His-Lys of selenium yeast, and specific method step is as follows：
(1) wine brewing ferment is carried out into shaking table culture, recycles first class seed pot, secondary seed tank and three-level seeding tank to expand step by step
Big culture, the culture feeding fermentation tank for obtaining, adds sodium selenite to carry out fermented and cultured, and selenium-rich ferment is obtained through centrifuge washing
It is female；
(2) Se-enriched yeast is digested using protease, concentration of substrate is that 12%, pH is maintained, and 3-4 obtains Se-enriched yeast
Enzymolysis liquid；Enzyme-added and enzymolysis time is different because concentration of substrate is different, and this technique determines that protein substrate concentration is 12%.
(3) by Se-enriched yeast enzymolysis liquid in 53-55 DEG C of temperature, self-dissolving 2 hours under natural ph add matter in the self-dissolving stage
The addition of wall release agent, wherein plasmolyticum is the 1-2% of Se-enriched yeast enzymolysis liquid weight；
Self-dissolving is by the endobacillary endogenous enzymes of yeast (protease, nuclease, carbohydrate inversion enzyme etc.), by thalline
The macromolecular substances Partial digestion such as cell membrane, reduces production cost, and produce flavor substance composition.
Above-mentioned plasmolyticum is a kind of or mixture of the two in sodium chloride or phosphate buffer.
(4) pH value of the Se-enriched yeast enzymolysis liquid after self-dissolving is adjusted to 6-7, the Optimal pH of Yeast protein enzyme effect is 5-
8, suitable pH value can accelerate to digest and make product special flavour unique；It is subsequently adding composite biological enzyme preparation, composite biological enzyme preparation
Addition for Se-enriched yeast weight 0.1-0.2%, obtain enzymolysis liquid stand-by；
The composite biological enzyme preparation mainly has the enzymes such as Lysozyme, restriction endonuclease, excision enzyme and food flavor enzyme, by any
Ratio is combined；The effect of wherein Lysozyme is dissolving cell membrane, and protein is split into peptides by restriction endonuclease, circumscribed
Macromolecular peptide is resolved into small-molecular peptides and partial amino-acid by enzyme, and food flavor enzyme produces the pure fragrance matter of nature；
(5) enzymolysis liquid is hydrolyzed 20-24 hours at 55 ± 2 DEG C of temperature, after enzymolysis, enzymolysis liquid is heated to 85 DEG C -90
DEG C, 2 hours are incubated, by the enzyme-deactivating in solution, it is ensured that product quality stabilization, high income, ultrafiltration is carried out afterwards, filtrate is collected,
Obtain polypeptide filtrate；
It is experimentally confirmed that hydrolysis time control is optimal at 20-24 hours, the time is too short, and protein hydrolysis is incomplete, time mistake
Long to decompose excessive, small peptide yield is low, and the production cycle, efficiency long was low.Because molecular weight is less than the protein of 1000 (i.e. below 9 peptides)
Oligopeptide just has efficient biological agent, and this technique is retained and divided using Ultra filtration membrane filtrate of the molecular weight less than 1000 of damming
Selenoprotein small peptide of the son amount below 1000.
(6) the polypeptide filtrate that will be obtained after ultrafiltration carries out nanofiltration desalination, obtains desalination peptide liquid；Due to being added in enzymolysis process
The inorganic salts such as NaCL, phosphate, the salts such as NaCL are sloughed using nanofiltration separation technique, by purifying products, remove invalid element, with
Exempt to influence products taste and effect, it is ensured that product quality.
(7) desalination peptide liquid concentrated, dried, you can obtained the oligomeric Gly-His-Lys of selenium yeast.
Wherein concentrate is carried out using low temperature condensing mode, dries the centrifugal spray drying mode that uses, and drying temperature is 50 DEG C,
The effective ingredient that will not be destroyed in product, can be completely secured the quality of product.
Calculated with Se-enriched yeast 48kg, the yield of the oligomeric Gly-His-Lys of selenium yeast is 18.8kg in the application, wherein selenium yeast is low
Poly- Gly-His-Lys Se content 686ug/g, selenium yeast oligopeptide powder purity 68% (content of selenium yeast protein small peptide), selenium yeast oligopeptide
Powder production yield 18.8/48=39.16%.
Such as the patent of invention of Patent No. CN01100600.5：Se-enriched yeast and its production method containing high-biomass,
The Se-enriched yeast preparation method of the application subsidy (1) can be carried out according to the method for above-mentioned patent
In the category of peptide, the molecular weight of polypeptide is more much greater than the molecular weight of oligopeptide, oligopeptide in human body almost
Can reach and be not required to digestion degree i.e. absorbed by the body, the digestion burden of stomach and intestine not increased, so for the nutrition of human body
Improvement result is very big, and especially for the bad crowd of digestive functions, and oligopeptide is have many for human body
Physiological function：1st, suppress cell degeneration, strengthen the immunity of human body；2nd, active cell activity, effectively removes harmful
Free radical；3rd, human body DC is repaired, improves the metabolism of cell；4th, promote, maintain cell normally metabolic
Function.Oligopeptide is remarkable protein supplements, is the composition of protein because it is allied substances substantially with protein
Unit, can supplement protein for human body, while the molecular weight very little of oligopeptide, can directly be absorbed by the body utilization, absorb
It is in hgher efficiency, the characteristics of reach 100% absorption.
The oligomeric Gly-His-Lys of selenium yeast of the application production, be the rich Se in products yeast with our company's production as raw material, using existing
For biotechnology, biological enzyme formulation is added, after Se-enriched yeast is converted through enzymolysis, then by modern point of ultrafiltration, nanofiltration etc.
From the oligomeric Gly-His-Lys of biological technology products-selenium yeast that purification technique is produced, the products taste is good, is extremely easy to human consumption's suction
Receive and utilize, can rapidly supplement protein, strengthen body immunity, there is antifatigue, anti-oxidant, beauty and skin care effect.
The oligomeric Gly-His-Lys of above-mentioned selenium yeast are used to be made selenium-rich cosmetics in cosmetics, old man's skin is prevented from old
Change, repair cell, treat senile plaque expelling.
The beneficial effects of the invention are as follows：
1st, the oligomeric Gly-His-Lys of selenium yeast are another high-tech biotechnology products that my company offers to market, have filled up this
The market vacancy of class product, protein small peptide can slow down the degraded of protein rapidly for human body is absorbed, normal egg in acceleration bodies
The synthesis supply of white matter, Organic Selenium is easily absorbed by the body to be utilized and human body is had no toxic side effect, and has significant physiology to protect to human body
Health-care function.
2nd, this product processes is unique, with high content of technology, strong innovation, and employing composite biological enzyme preparation carries out enzyme
Solution, simplifies production process, improves production efficiency；Apply current international advanced molecule membrane separation technique and isolate and purify product
Product.The application of new material new technology has fully ensured that the stabilization of product quality.
3rd, the protein hydrolysis generation after selenium yeast broken wall (is contained into 3-9 seleno-amino acids molecule with selenoprotein oligopeptide
Composition) based on, and the compound containing the composition such as nucleotides, amino acid, B family vitamin, mineral matter, then by ultrafiltration, nanofiltration, dense
The Particular crafts such as contracting, centrifugal drying are refined to be formed, and product processes are advanced, are rich in novelty, take full advantage of international forward position
Biological enzymolysis technology, ultrafiltration, nanofiltration separation purifying etc. new material new technology, filled up the market vacancy of this product.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment 1
A kind of utilization Se-enriched yeast prepares the technique of the oligomeric Gly-His-Lys of selenium yeast, and specific method step is as follows：
(1) wine brewing ferment is carried out into shaking table culture, recycles first class seed pot, secondary seed tank and three-level seeding tank to expand step by step
Big culture, the culture feeding fermentation tank for obtaining, adds sodium selenite to carry out fermented and cultured, and selenium-rich ferment is obtained through centrifuge washing
It is female；
(2) Se-enriched yeast is digested using protease, concentration of substrate is that 12%, pH is maintained, 4, obtains Se-enriched yeast enzyme
Solution liquid；Enzyme-added and enzymolysis time is different because concentration of substrate is different, and this technique determines that protein substrate concentration is 12%.
(3) by Se-enriched yeast enzymolysis liquid in 55 DEG C of temperature, self-dissolving 2 hours under natural ph add matter wall in the self-dissolving stage
The addition of release agent, wherein plasmolyticum is the 1% of Se-enriched yeast enzymolysis liquid weight；
Self-dissolving is by the endobacillary endogenous enzymes of yeast (protease, nuclease, carbohydrate inversion enzyme etc.), by thalline
The macromolecular substances Partial digestion such as cell membrane, reduces production cost, and produce flavor substance composition.
Above-mentioned plasmolyticum is a kind of or mixture of the two in sodium chloride or phosphate buffer.
(4) pH value of the Se-enriched yeast enzymolysis liquid after self-dissolving is adjusted to 7, the Optimal pH of Yeast protein enzyme effect is 5-8,
Suitable pH value can accelerate to digest and make product special flavour unique；Composite biological enzyme preparation is subsequently adding, composite biological enzyme preparation
Addition is the 0.2% of Se-enriched yeast weight, obtains enzymolysis liquid stand-by；
The composite biological enzyme preparation mainly has the enzymes such as Lysozyme, restriction endonuclease, excision enzyme and food flavor enzyme, by any
Ratio is combined；The effect of wherein Lysozyme is dissolving cell membrane, and protein is split into peptides by restriction endonuclease, circumscribed
Macromolecular peptide is resolved into small-molecular peptides and partial amino-acid by enzyme, and food flavor enzyme produces the pure fragrance matter of nature；
(5) enzymolysis liquid is hydrolyzed 20-24 hours at 55 ± 2 DEG C of temperature, after enzymolysis, enzymolysis liquid is heated to 85 DEG C -90
DEG C, 2 hours are incubated, by the enzyme-deactivating in solution, it is ensured that product quality stabilization, high income, ultrafiltration is carried out afterwards, filtrate is collected,
Obtain polypeptide filtrate；
It is experimentally confirmed that hydrolysis time control is optimal at 20-24 hours, the time is too short, and protein hydrolysis is incomplete, time mistake
Long to decompose excessive, small peptide yield is low, and the production cycle, efficiency long was low.Because molecular weight is less than the protein of 1000 (i.e. below 9 peptides)
Oligopeptide just has efficient biological agent, and this technique is retained and divided using Ultra filtration membrane filtrate of the molecular weight less than 1000 of damming
Selenoprotein small peptide of the son amount below 1000.
(6) the polypeptide filtrate that will be obtained after ultrafiltration carries out nanofiltration desalination, obtains desalination peptide liquid；Due to being added in enzymolysis process
The inorganic salts such as NaCL, phosphate, the salts such as NaCL are sloughed using nanofiltration separation technique, by purifying products, remove invalid element, with
Exempt to influence products taste and effect, it is ensured that product quality.
(7) desalination peptide liquid concentrated, dried, you can obtained the oligomeric Gly-His-Lys of selenium yeast.
Wherein concentrate is carried out using low temperature condensing mode, dries the centrifugal spray drying mode that uses, and drying temperature is 50 DEG C,
The effective ingredient that will not be destroyed in product, can be completely secured the quality of product.
Calculated with Se-enriched yeast 48kg, the yield of the oligomeric Gly-His-Lys of selenium yeast is 18.8kg in the application, wherein selenium yeast is low
Poly- Gly-His-Lys Se content 686ug/g, selenium yeast oligopeptide powder purity 68% (content of selenium yeast protein small peptide), selenium yeast oligopeptide
Powder production yield 18.8/48=39.16%.
Embodiment 2
A kind of utilization Se-enriched yeast prepares the technique of the oligomeric Gly-His-Lys of selenium yeast, and specific method step is as follows：
(1) Se-enriched yeast is digested using protease, concentration of substrate is that 12%, pH is maintained, 3, obtains Se-enriched yeast enzyme
Solution liquid；Enzyme-added and enzymolysis time is different because concentration of substrate is different, and this technique determines that protein substrate concentration is 12%.
(2) by Se-enriched yeast enzymolysis liquid in 53 DEG C of temperature, self-dissolving 2 hours under natural ph add matter wall in the self-dissolving stage
The addition of release agent, wherein plasmolyticum is the 2% of Se-enriched yeast enzymolysis liquid weight；
Self-dissolving is by the endobacillary endogenous enzymes of yeast (protease, nuclease, carbohydrate inversion enzyme etc.), by thalline
The macromolecular substances Partial digestion such as cell membrane, reduces production cost, and produce flavor substance composition.
Above-mentioned plasmolyticum is a kind of or mixture of the two in sodium chloride or phosphate buffer.
(3) pH value of the Se-enriched yeast enzymolysis liquid after self-dissolving is adjusted to 6, the Optimal pH of Yeast protein enzyme effect is 5-8,
Suitable pH value can accelerate to digest and make product special flavour unique；Composite biological enzyme preparation is subsequently adding, composite biological enzyme preparation
Addition is the 0.1% of Se-enriched yeast weight, obtains enzymolysis liquid stand-by；
The composite biological enzyme preparation mainly has the enzymes such as Lysozyme, restriction endonuclease, excision enzyme and food flavor enzyme, by any
Ratio is combined；The effect of wherein Lysozyme is dissolving cell membrane, and protein is split into peptides by restriction endonuclease, circumscribed
Macromolecular peptide is resolved into small-molecular peptides and partial amino-acid by enzyme, and food flavor enzyme produces the pure fragrance matter of nature；
(4) enzymolysis liquid is hydrolyzed 20-24 hours at 55 ± 2 DEG C of temperature, after enzymolysis, enzymolysis liquid is heated to 85 DEG C -90
DEG C, 2 hours are incubated, by the enzyme-deactivating in solution, it is ensured that product quality stabilization, high income, ultrafiltration is carried out afterwards, filtrate is collected,
Obtain polypeptide filtrate；
It is experimentally confirmed that hydrolysis time control is optimal at 20-24 hours, the time is too short, and protein hydrolysis is incomplete, time mistake
Long to decompose excessive, small peptide yield is low, and the production cycle, efficiency long was low.Because molecular weight is less than the protein of 1000 (i.e. below 9 peptides)
Oligopeptide just has efficient biological agent, and this technique is retained and divided using Ultra filtration membrane filtrate of the molecular weight less than 1000 of damming
Selenoprotein small peptide of the son amount below 1000.
(5) the polypeptide filtrate that will be obtained after ultrafiltration carries out nanofiltration desalination, obtains desalination peptide liquid；Due to being added in enzymolysis process
The inorganic salts such as NaCL, phosphate, the salts such as NaCL are sloughed using nanofiltration separation technique, by purifying products, remove invalid element, with
Exempt to influence products taste and effect, it is ensured that product quality.
(6) desalination peptide liquid concentrated, dried, you can obtained the oligomeric Gly-His-Lys of selenium yeast.
Wherein concentrate is carried out using low temperature condensing mode, dries the centrifugal spray drying mode that uses, and drying temperature is 50 DEG C,
The effective ingredient that will not be destroyed in product, can be completely secured the quality of product.
Selenium-rich Saccharomyces cerevisiae uses selenium yeast powder high, and the selenium yeast powder high is that selenium yeast high slurry is made into dry powder, its
Middle selenium yeast powder Se content high is 1560ug/g.Calculated with Se-enriched yeast 48kg, the yield of the oligomeric Gly-His-Lys of selenium yeast in the application
It is 18.8kg, the wherein oligomeric Gly-His-Lys Se content 686ug/g of selenium yeast, (selenium yeast albumen is small for selenium yeast oligopeptide powder purity 68%
The content of peptide), the oligomeric Gly-His-Lys production yield 18.8/48=39.16% of selenium yeast.
General principle of the invention and principal character and advantages of the present invention has been shown and described above.The technology of the industry
Personnel it should be appreciated that the present invention is not limited to the above embodiments, simply explanation described in above-described embodiment and specification this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appending claims and its
Equivalent thereof.
Claims (8)
1. the technique that a kind of utilization Se-enriched yeast prepares the oligomeric Gly-His-Lys of selenium yeast, it is characterised in that specific method step is as follows：
(1) wine brewing ferment is carried out into shaking table culture, recycles first class seed pot, secondary seed tank and three-level seeding tank to expand training step by step
Support, the culture feeding fermentation tank for obtaining adds sodium selenite to carry out fermented and cultured, and Se-enriched yeast is obtained through centrifuge washing；
(2) Se-enriched yeast is digested using protease, concentration of substrate is that 12%, pH is maintained, 3-4, obtains Se-enriched yeast enzymolysis
Liquid；
(3) by Se-enriched yeast enzymolysis liquid in 53-55 DEG C of temperature, self-dissolving 2 hours under natural ph add matter wall point in the self-dissolving stage
From agent, the wherein addition of plasmolyticum is the 1-2% of Se-enriched yeast enzymolysis liquid weight；Above-mentioned plasmolyticum is sodium chloride
Or a kind of or mixture of the two in phosphate buffer；
(4) pH value of the Se-enriched yeast enzymolysis liquid after self-dissolving is adjusted to 6-7, is subsequently adding composite biological enzyme preparation, be combined life
The addition of thing enzyme preparation is the 0.1-0.2% of Se-enriched yeast weight, obtains enzymolysis liquid stand-by；
(5) enzymolysis liquid is hydrolyzed 20-24 hour at 55 ± 2 DEG C of temperature, after enzymolysis, enzymolysis liquid is heated to 85 DEG C -90 DEG C, guarantor
Temperature 2 hours, carries out ultrafiltration afterwards, collects filtrate, obtains polypeptide filtrate；
(6) the polypeptide filtrate that will be obtained after ultrafiltration carries out nanofiltration desalination, obtains desalination peptide liquid；
(7) desalination peptide liquid concentrated, dried, you can obtained the oligomeric Gly-His-Lys of selenium yeast.
2. the technique that utilization Se-enriched yeast according to claim 1 prepares the oligomeric Gly-His-Lys of selenium yeast, it is characterised in that above-mentioned
Concentration is carried out using low temperature condensing mode in step (7).
3. the technique that utilization Se-enriched yeast according to claim 1 prepares the oligomeric Gly-His-Lys of selenium yeast, it is characterised in that above-mentioned
Dried in step (7) and use centrifugal spray drying mode.
4. the technique that utilization Se-enriched yeast according to claim 3 prepares the oligomeric Gly-His-Lys of selenium yeast, it is characterised in that described
The drying temperature of centrifugal drying is 50 DEG C.
5. the technique that utilization Se-enriched yeast according to claim 1 prepares the oligomeric Gly-His-Lys of selenium yeast, it is characterised in that described
Composite biological enzyme preparation is to be combined to form in any proportion by Lysozyme, restriction endonuclease, excision enzyme and food flavor enzyme.
6. the technique that utilization Se-enriched yeast according to claim 1 prepares the oligomeric Gly-His-Lys of selenium yeast, it is characterised in that described
Se-enriched yeast is selenium yeast powder high, wherein selenium yeast powder Se content high is 1560ug/g.
7. one kind oligomeric Gly-His-Lys of selenium yeast as obtained in claim any one of 1-6, its Se content 686ug/g.
8. a kind of application of the oligomeric Gly-His-Lys of selenium yeast in cosmetics as obtained in claim any one of 1-6.
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| CN110387395A (en) * | 2019-08-23 | 2019-10-29 | 武汉轻工大学 | The preparation method and selenium-rich solid beverage of mushroom with abundant selenium protein peptide powder |
| CN110643665A (en) * | 2019-10-29 | 2020-01-03 | 恩施道之道生物科技有限公司 | Method for extracting selenoprotein from selenium-enriched yeast by double-enzyme coupling method |
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| CN114214216A (en) * | 2021-10-11 | 2022-03-22 | 厦门海嘉成生物科技有限公司 | Selenium-rich yeast strain and application thereof |
| CN114853855A (en) * | 2022-04-14 | 2022-08-05 | 安徽省华信生物药业股份有限公司 | Chromium-rich yeast active peptide with function of promoting secretion of hypoglycemic hormone GLP-1 |
| CN115737454A (en) * | 2022-11-30 | 2023-03-07 | 何直源 | Double-bacterium composite extract and preparation method and application thereof |
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| CN109043544A (en) * | 2018-07-06 | 2018-12-21 | 恩施硒谷科技股份有限公司 | A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin |
| CN110387395A (en) * | 2019-08-23 | 2019-10-29 | 武汉轻工大学 | The preparation method and selenium-rich solid beverage of mushroom with abundant selenium protein peptide powder |
| CN110643665A (en) * | 2019-10-29 | 2020-01-03 | 恩施道之道生物科技有限公司 | Method for extracting selenoprotein from selenium-enriched yeast by double-enzyme coupling method |
| CN113308381A (en) * | 2021-05-10 | 2021-08-27 | 江苏中农科食品工程股份有限公司 | Pleurotus ostreatus strain ZNK-MS-08, culture method, pleurotus ostreatus culture medium and culture medium additive |
| CN113308381B (en) * | 2021-05-10 | 2022-06-28 | 江苏中农科食品工程股份有限公司 | Pleurotus ostreatus strain ZNK-MS-08, culture method, pleurotus ostreatus culture medium and culture medium additive |
| CN113679052A (en) * | 2021-08-27 | 2021-11-23 | 湖北安琪生物集团有限公司 | Method for preparing food-grade yeast protein peptide through multiple stress environment |
| CN114214216A (en) * | 2021-10-11 | 2022-03-22 | 厦门海嘉成生物科技有限公司 | Selenium-rich yeast strain and application thereof |
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| CN114853855A (en) * | 2022-04-14 | 2022-08-05 | 安徽省华信生物药业股份有限公司 | Chromium-rich yeast active peptide with function of promoting secretion of hypoglycemic hormone GLP-1 |
| CN114853855B (en) * | 2022-04-14 | 2023-06-20 | 安徽省华信生物药业股份有限公司 | Chromium-enriched yeast active peptide with function of promoting and reducing secretion of blood glucose hormone GLP-1 |
| CN115737454A (en) * | 2022-11-30 | 2023-03-07 | 何直源 | Double-bacterium composite extract and preparation method and application thereof |
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