CN106754699B - miRNA-155及其抑制剂在DC-CIK细胞培养方面的应用 - Google Patents
miRNA-155及其抑制剂在DC-CIK细胞培养方面的应用 Download PDFInfo
- Publication number
- CN106754699B CN106754699B CN201611194975.8A CN201611194975A CN106754699B CN 106754699 B CN106754699 B CN 106754699B CN 201611194975 A CN201611194975 A CN 201611194975A CN 106754699 B CN106754699 B CN 106754699B
- Authority
- CN
- China
- Prior art keywords
- cells
- mirna
- cik
- inhibitor
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
- C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/65—MicroRNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
- C12N2502/1121—Dendritic cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611194975.8A CN106754699B (zh) | 2016-12-22 | 2016-12-22 | miRNA-155及其抑制剂在DC-CIK细胞培养方面的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611194975.8A CN106754699B (zh) | 2016-12-22 | 2016-12-22 | miRNA-155及其抑制剂在DC-CIK细胞培养方面的应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106754699A CN106754699A (zh) | 2017-05-31 |
CN106754699B true CN106754699B (zh) | 2020-01-31 |
Family
ID=58899492
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201611194975.8A Active CN106754699B (zh) | 2016-12-22 | 2016-12-22 | miRNA-155及其抑制剂在DC-CIK细胞培养方面的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106754699B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109122662B (zh) * | 2018-08-27 | 2021-01-29 | 深圳华大基因细胞科技有限责任公司 | 一种人胎盘间充质干细胞运输液 |
CN109042627B (zh) * | 2018-08-27 | 2021-08-10 | 南京赛尔健生物技术有限公司 | 一种人羊水间充质干细胞运输液 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101472470A (zh) * | 2006-04-24 | 2009-07-01 | 俄亥俄州立大学研究基金会 | miR155转基因小鼠中前B细胞增殖和淋巴母细胞性白血病/高度淋巴瘤 |
-
2016
- 2016-12-22 CN CN201611194975.8A patent/CN106754699B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101472470A (zh) * | 2006-04-24 | 2009-07-01 | 俄亥俄州立大学研究基金会 | miR155转基因小鼠中前B细胞增殖和淋巴母细胞性白血病/高度淋巴瘤 |
Non-Patent Citations (3)
Title |
---|
"DC-CIK细胞的生物学活性及体外抗白血病作用的研究";魏绪仓等;《中国实验血液学杂志》;20081230;第16卷(第5期);第1150页摘要部分 * |
"Effect of DC-CIK cell on the proliferation, apoptosis and ";Hui-Qing Qu et al.,;《Asian Pacific Journal of Tropical Medicine》;20140830;第7卷(第8期);第659-662页 * |
"抑制miR-155表达对白血病细胞系THP-1的增殖和凋亡影响及作用机制";薛华等;《中国实验血液学杂志》;20141230;第22卷(第6期);第1554页左栏第4段-右栏第1段 * |
Also Published As
Publication number | Publication date |
---|---|
CN106754699A (zh) | 2017-05-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109294985B (zh) | 一种用于nk细胞体外扩增的培养基体系及nk细胞体外扩增的方法 | |
CN106472486B (zh) | 一种维持dc-cik细胞高杀伤力的冻存保护剂 | |
CN102321581B (zh) | 腹水肿瘤细胞致敏dc-cik的制备方法 | |
CN107929727A (zh) | 一种新的树突状细胞疫苗的制备方法 | |
Prechtel et al. | Small interfering RNA (siRNA) delivery into monocyte-derived dendritic cells by electroporation | |
CN104815323B (zh) | 一种树突状细胞肿瘤疫苗及其制备方法 | |
EP2881462B1 (en) | Method for obtaining monocytes or nk cells | |
Yang et al. | Characteristics of NK cells from leukemic microenvironment in MLL-AF9 induced acute myeloid leukemia | |
CN106754699B (zh) | miRNA-155及其抑制剂在DC-CIK细胞培养方面的应用 | |
CN108060129A (zh) | 调节性t细胞体外扩增方法 | |
CN110438077B (zh) | 一种NK与γδT细胞的同时培养方法 | |
CN113403273B (zh) | 一种扩增脐带血来源的nk细胞的培养方法 | |
Delirezh et al. | Generation of mature monocyte-derived dendritic cells in the presence of heparin and monocyte conditioned medium: phenotypic and functional comparison | |
CN103173410B (zh) | 用于刺激树突状细胞成熟的组合物以及方法 | |
CN114149970B (zh) | 一种外周血造血干细胞来源致敏树突状细胞的制备方法及应用 | |
CN116574679A (zh) | 特异性nk细胞的制备方法及其应用 | |
CN115094034B (zh) | 一种人nkt细胞系及其应用 | |
CN113564115B (zh) | 一种高扩增型dc-cik细胞及其制备和应用 | |
CN110643575A (zh) | 成熟树突状细胞的培养试剂及培养方法 | |
GanjiBakhsh et al. | Mixture of fibroblast, epithelial and endothelial cells conditioned media induce monocyte-derived dendritic cell maturation | |
CN110607275B (zh) | 一种增强型自然杀伤细胞的培养方法 | |
CN108251370B (zh) | 非细胞来源的多肽致敏的dc-cik细胞、其构建方法及应用 | |
CN109576220B (zh) | 一种灭活饲养细胞联合细胞因子刺激nk细胞扩增的方法 | |
CN106581065A (zh) | miRNA‑146a及其抑制剂在提高CIK细胞杀伤力方面的应用 | |
CN106577635B (zh) | 一种维持cik细胞高杀伤力的冻存保护剂 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20191231 Address after: 510000 Room D09, 4th Floor, 131 Airport Road, Baiyun District, Guangzhou City, Guangdong Province Applicant after: Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. Address before: No. 9 Zhongshan Science Park by the road in Liuhe District of Nanjing City, Jiangsu province 211505 Applicant before: NANJING BIOTEKE BIOTECHNOLOGY CO.,LTD. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP02 | Change in the address of a patent holder |
Address after: Building F2, 39 Ruihe Road, Huangpu District, Guangzhou City, Guangdong Province 510000 Patentee after: Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. Address before: 510000 Room D09, 4th Floor, 131 Airport Road, Baiyun District, Guangzhou City, Guangdong Province Patentee before: Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. |
|
CP02 | Change in the address of a patent holder | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Application of miRNA-155 and its inhibitors in DC-CIK cell culture Effective date of registration: 20230629 Granted publication date: 20200131 Pledgee: Hunan Pingjiang Rural Commercial Bank Co.,Ltd. Pledgor: Chen Zhongping|Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. Registration number: Y2023980046730 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |