CN106754343A - DNA sequencing apparatus and system based on pyrosequencing - Google Patents
DNA sequencing apparatus and system based on pyrosequencing Download PDFInfo
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- CN106754343A CN106754343A CN201710061926.5A CN201710061926A CN106754343A CN 106754343 A CN106754343 A CN 106754343A CN 201710061926 A CN201710061926 A CN 201710061926A CN 106754343 A CN106754343 A CN 106754343A
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CN201710061926.5A Active CN106754343B (en) | 2017-01-12 | 2017-01-27 | DNA sequencing apparatus and system based on pyrosequencing |
CN201710061937.3A Active CN106591107B (en) | 2017-01-12 | 2017-01-27 | Sample adding device for pyrosequencing |
CN201710062270.9A Pending CN106754313A (en) | 2017-01-12 | 2017-02-02 | Nucleic acid sequencing apparatus and system |
CN201710076466.3A Active CN107299054B (en) | 2017-01-12 | 2017-02-13 | Control system and control method of DNA sequencing device |
CN201710086669.0A Active CN107312710B (en) | 2017-01-12 | 2017-02-17 | DNA sequencing device based on pyrosequencing and sequencing method thereof |
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CN201710076466.3A Active CN107299054B (en) | 2017-01-12 | 2017-02-13 | Control system and control method of DNA sequencing device |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101596469A (en) * | 2009-07-03 | 2009-12-09 | 烟台艾德康生物科技有限公司 | Multi-channel loading device |
CN102212466A (en) * | 2011-04-11 | 2011-10-12 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Alkaline lysis system for preparing plasmid DNA and combined system |
CN103205357A (en) * | 2013-04-14 | 2013-07-17 | 上海聚阵生物科技有限公司 | Micro sample injection method and device for pyrophosphate nucleic acid sequencing system as well as application of micro sample injection method and device |
CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6423536B1 (en) * | 1999-08-02 | 2002-07-23 | Molecular Dynamics, Inc. | Low volume chemical and biochemical reaction system |
CN2478110Y (en) * | 2001-04-25 | 2002-02-20 | 张宏 | Full automatic gene sequencing instrument |
JP2005295877A (en) * | 2004-04-09 | 2005-10-27 | Taiyo Yuden Co Ltd | Method for analyzing nucleic acid, analyzer and disk for analysis |
KR101481054B1 (en) * | 2011-11-15 | 2015-01-14 | 한국기계연구원 | A device for automatically analyzing nucleic acid |
CN102517206B (en) * | 2011-12-31 | 2015-06-03 | 盛司潼 | Gene sequencing device and system |
CN102707078B (en) * | 2012-05-24 | 2013-09-25 | 中国科学院北京基因组研究所 | Reagent supply system for DNA (deoxyribonucleic acid) sequencer and control method |
CN102703301A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera |
CN102703314B (en) * | 2012-05-24 | 2013-09-04 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
CN103789446B (en) * | 2014-02-27 | 2015-10-28 | 厦门大学附属中山医院 | Manganic pyrophosphate complex initiation clopidogrel personalized medicine genetic polymorphism detection test kit and detection method thereof |
CN104777321B (en) * | 2015-04-16 | 2016-07-06 | 清华大学 | Chemiluminescence immune assay device |
-
2017
- 2017-01-27 CN CN201710061927.XA patent/CN106754292B/en active Active
- 2017-01-27 CN CN201710061926.5A patent/CN106754343B/en active Active
- 2017-01-27 CN CN201710061937.3A patent/CN106591107B/en active Active
- 2017-02-02 CN CN201710062270.9A patent/CN106754313A/en active Pending
- 2017-02-13 CN CN201710076466.3A patent/CN107299054B/en active Active
- 2017-02-17 CN CN201710086669.0A patent/CN107312710B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101596469A (en) * | 2009-07-03 | 2009-12-09 | 烟台艾德康生物科技有限公司 | Multi-channel loading device |
CN102212466A (en) * | 2011-04-11 | 2011-10-12 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Alkaline lysis system for preparing plasmid DNA and combined system |
CN103205357A (en) * | 2013-04-14 | 2013-07-17 | 上海聚阵生物科技有限公司 | Micro sample injection method and device for pyrophosphate nucleic acid sequencing system as well as application of micro sample injection method and device |
CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107179410A (en) * | 2017-07-04 | 2017-09-19 | 北京军秀咨询有限公司 | A kind of DNA detection means and detection method |
US10620123B2 (en) | 2018-02-08 | 2020-04-14 | Delta Electronics Int'l (Singapore) Pte Ltd | Fluorescence detection instrument |
CN114453146A (en) * | 2022-04-12 | 2022-05-10 | 深圳人体密码基因科技有限公司 | Centrifugal device for gene detection of self-separation solution |
CN114453146B (en) * | 2022-04-12 | 2022-06-14 | 深圳人体密码基因科技有限公司 | Centrifugal device for gene detection of self-separation solution |
CN117050867A (en) * | 2023-08-20 | 2023-11-14 | 浙江深华生物科技有限公司 | High-throughput quantitative detection system for evaluating tumor DNA |
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CN107299054B (en) | 2020-09-01 |
CN107299054A (en) | 2017-10-27 |
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CN106591107B (en) | 2019-04-12 |
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CN106754313A (en) | 2017-05-31 |
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CN107312710B (en) | 2021-06-25 |
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