CN106748195B - The fertilizer and its preparation technology of a kind of growth substance containing natural plants - Google Patents
The fertilizer and its preparation technology of a kind of growth substance containing natural plants Download PDFInfo
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- CN106748195B CN106748195B CN201611238205.9A CN201611238205A CN106748195B CN 106748195 B CN106748195 B CN 106748195B CN 201611238205 A CN201611238205 A CN 201611238205A CN 106748195 B CN106748195 B CN 106748195B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention belongs to technical field of fertilizers, and more particularly to the fertilizer and its preparation technology of a kind of growth substance containing natural plants, the preparation technology of the fertilizer of the growth substance containing natural plants comprises the following steps：S1：Low temperature classification extraction is carried out to the sea-tangle after crushing, leaching liquor is merged, concentrated；S2：Add water to carry out classification enzymolysis with cellulase, pectase and fucosidase successively the sea-tangle after extraction, obtain enzymolysis liquid；S3：The pH value of enzymolysis liquid is adjusted, merges with extraction concentrate, concentrate, produce.The fertilizer of the growth substance provided by the invention containing natural plants contains abundant bioactive substance, can effectively facilitate growing for crop；Meanwhile preparation is simple for the fertilizer of the growth substance of the invention containing natural plants, can effectively extract and retain the bioactive substance in sea-tangle, production cost is low, and production technology is easily controllable, is advantageous to large-scale industrialized production.
Description
Technical field
The invention belongs to technical field of fertilizers, and in particular to the fertilizer and its preparation work of a kind of growth substance containing natural plants
Skill.
Background technology
There is the natural plants growth substance that can promote plant growth in marine alga, can as the growth regulator of plant,
Using natural seaweed as raw material, the active principle in marine alga is extracted, that produces contains a variety of lifes such as alginic acid, Endogenous hormones
The dissolved fertilizer of active substances, it is a kind of novel agricultural green organic manure.Sea-tangle is the biostimulant production of marine alga source
In one of the most frequently used raw material.
Sea-tangle belongs to cold aqueous marine alga, is a kind of raw brown alga plant in large-scale sea grown in low temperature seawater, in the Liao Dynasty of China
Rather, there is cultivation in Shandong, Jiangsu, Zhejiang, Fujian and Guangdong Province, but the sea-tangle grown naturally only comes across the Huang to the north of Qingdao
Sea, the band of the Bohai Sea one.Sea-tangle is both a kind of very high vegetables of nutritive value, is the good raw material of marine alga fertilizer and Feed Manufacturing again.
Because growing environment is special, sea-tangle contain the trace element such as the iodine that many terrestrial organisms do not have, potassium, magnesium, manganese, zinc, titanium and
Algal polysaccharides, mannitol etc..In addition, also contain substantial amounts of high active ingredient and natural biological stimulin in studies have shown that sea-tangle,
Such as auximone, gibberellin, the basic element of cell division, abscisic acid, polyphenolic substance, these materials can stimulate non-in plant
The generation of the activity specific factor and the balance for adjusting endogenous hormones, can promote crop growth, improve yield, improve product
A variety of degeneration-resistant functions such as matter, enhancing crop drought resistance, cold-resistant, Resistant etc..
During using laminaria production marine alga source biostimulant, on the one hand clear up frond, using up cell membrane can
Can reach completely broken, so make intracellular inclusion discharge and obtain into the cell by macromolecular substances degraded, be easy to
The small-molecule substance being absorbed and used；On the other hand plant hormone therein is effectively maintained.In early stage marine alga source, biology pierces
In hormone production technology, people excessively pay attention to the extraction of the nutriments such as polysaccharide, mineral matter element and trace element therein, general
All over the extracting mode using high temperature, high pressure, the plant hormone for causing to contain in marine alga is destroyed, the fertilizer pair produced
The growth promoting function of crop is not notable.
The A of Chinese patent CN 104177136 disclose a kind of method that fertilizer active material is extracted from marine alga, marine alga
Dry powder can extract through techniques such as homogeneous, alcohol extracting, water extraction, the extraction of cold alkali lye, cellulose-albumen complex enzyme zymohydrolysis, Ultra filtration membranes
The active ingredients such as algal polysaccharides, alginic acid, chelated microelements are used as marine alga fertilizer production.It the method increase algal polysaccharides
With the recovery rate of alginic acid, but extracting method is complicated, and energy consumption is big, and cost is high, and low to the recovery rate of plant hormone.
The method for preparing marine alga source biostimulant at present mainly has 3 kinds of physicomechanical processes, chemical leaching test and bioanalysis.
Physicomechanical processes mainly make breaking-wall cell using modernization physical technique such as homogeneous, ultrasound, grinding and osmotic shock etc., but
The operation cell-wall breaking ratio such as matter, grinding is low, and the natural plants growth substance of free state can not discharge, and polishing is in its work
Cause production cost height due to having always a demand for cooling down in skill flow；And supersonic wave wall breaking technical costs is high, and in factory's metaplasia
It is not easy to implement in production；The target compound purity of osmotic shock extraction is high, but recovery rate is low, troublesome in poeration and cost is high.In addition,
Physicomechanical processes are in extraction process because physical mechanical effect can produce substantial amounts of heat and influence extraction efficiency.Chemical extraction
Method refers generally to acid, alkali hydrolysis method, marine alga is degraded under the high temperature conditions using acid or alkaline matter, can extract to obtain height and contains
The alginic acid and its esters of amount, but the hydrolysis of the long-time of high temperature action and soda acid can cause brokenly ring to a part of plant hormone, make
It loses activity, it is impossible to fully extracts plant hormone.Bioanalysis refers mainly to microbe fermentation method, refers to and is added in marine alga through sieving
The suitable micro-organisms bacterium of choosing, fermented mode obtain extract solution, but because the screening process of microorganism is more complicated and cumbersome,
It is not easy to implement in actual production and application.
Therefore, the method for extracting natural plants growth substance from sea-tangle in the prior art still has much room for improvement, how maximum
It is the improved key point of sea-tangle extraction process to extract to degree the various growth promoting activity compositions in sea-tangle.
The content of the invention
It is an object of the invention to overcome existing technological deficiency, there is provided a kind of fertilizer of growth substance containing natural plants and
Its preparation technology.The fertilizer of the growth substance provided by the invention containing natural plants, contain abundant alginic acid, alga oligosaccharides, thin
The bioactive substances such as born of the same parents' mitogen, abscisic acid, gibberellin and mannitol, growing for crop can be effectively facilitated；Meanwhile this
Preparation is simple for the fertilizer of invention growth substance containing natural plants, and the biology that can effectively extract and retain in sea-tangle is living
Property material, production cost is low, and production technology is easily controllable, is advantageous to large-scale industrialized production.
Technical scheme is as follows：
A kind of preparation technology of the fertilizer of growth substance containing natural plants, comprises the following steps：
S1：Dry, crush after sea-tangle impurity elimination is cleaned, add the calgon and 3~8 ‰ of its quality 0.5~2 ‰
Sapindoside, solid-liquid ratio 1 is pressed after mixing：(3~5) g/mL add water temperature be 25~35 DEG C under conditions of extraction 60~
90min, filter to obtain leaching liquor I；Sea-tangle after filtering is pressed into solid-liquid ratio 1：(2~4) g/mL adds water in temperature to be 25~35 DEG C
Under the conditions of extract 30~60min, filter to obtain leaching liquor II, it is standby to retain kelp residue；Leaching liquor I and leaching liquor II is merged, mixed
The 1/5~1/3 of original volume is concentrated under reduced pressure into after even, obtains extraction concentrated liquor；
S2：Kelp residue is pressed into solid-liquid ratio 1：(8~12) g/mL adds water to mix, and adds cellulase, is 50~65 in temperature
DEG C, pH value be 5.8~6.5 under conditions of digest 1~2h；Add pectase, temperature be 45~60 DEG C, pH value be 3.0~
0.75~1.5h is digested under conditions of 5.0；Be eventually adding fucosidase, temperature be 45~60 DEG C, pH value be 5.5~7.0
Under conditions of digest 0.75~1.5h, filtering, obtain enzymolysis liquid；
S3：The pH value for adjusting enzymolysis liquid is 6.8~7.2, adds and extracts concentrated liquor described in step S1, is depressurized after mixing dense
The 2/3~4/5 of original volume is reduced to, produces the fertilizer of the growth substance containing natural plants.
Preferably, the dosage for the cellulase that step S2 is added is the 1.2~1.8% of kelp residue quality.
Preferably, the dosage for the pectase that step S2 is added is the 1.2~1.8% of kelp residue quality.
Preferably, the dosage for the fucosidase that step S2 is added is the 0.4~0.6% of kelp residue quality.
In the preparation technology of the fertilizer of the growth substance provided by the invention containing natural plants, on the one hand, in leaching process
Calgon and sapindoside are added, cell permeability of the membrane can be changed and change the osmotic pressure in cell membrane, promote to have
Imitate extravasation；On the other hand, the resorption of active ingredient in sea-tangle in leaching process can be prevented using low temperature classification extraction, so as to
The leaching rate of the Endogenous hormones such as gibberellin in sea-tangle, the basic element of cell division and abscisic acid, mannitol etc. is effectively improved, meanwhile,
Leaching liquor is separated with kelp residue, can avoid subsequently carrying out sea-tangle enzymolysis processing process to Endogenous hormones and mannitol etc.
The destruction of bioactive ingredients, the bioactive ingredients such as Endogenous hormones in sea-tangle, mannitol are effectively retained, improved in fertilizer
The content of natural plants growth substance.
During the experiment of cell membrane degree of crushing is improved, inventor in enzymolysis process it was unexpectedly observed that apply rock algae
Glycosidase, cell membrane can be effectively facilitated and crushed, promote intracellular natural plants growth substance to discharge, improved and naturally planted in fertilizer
The content of thing growth substance；Meanwhile inventor has found through lot of experiments, it is classified using cellulase-pectase-fucosidase
Zymolysis technique, cell membrane can be made broken complete, intracellular inclusion is discharged and is obtained thin
It is that intracellular is degraded by macromolecular substances, be easy to the small-molecule substance that is absorbed and used, and due to enzymolysis process mild condition, therefore
The natural plants growth substance in sea-tangle can farthest be retained, acidolysis, alkaline hydrolysis and high temperature is overcome and natural plants are given birth to
The destruction of long species activity, the content of natural plants growth substance in fertilizer is set to be greatly improved.
After testing, natural plants are contained made from the preparation technology of the fertilizer of the growth substance provided by the invention containing natural plants
The fertilizer of growth substance, its natural plants growth substance species mainly contained and its content are as follows：18~25mg/g of alginic acid；
12~18mg/g of alga oligosaccharides；The μ g/L of abscisic acid 0.3~0.4；The μ g/L of gibberellin 100~110；The μ of the basic element of cell division 140~160
g/L；37~42g/L of 9~12g/L of mannitol and organic matter.
The present invention also provides a kind of water-soluble fertilizer of growth substance containing natural plants, and it includes following composition：Institute of the present invention
The fertilizer of the growth substance containing natural plants stated, a great number of elements raw material and/or trace element materials and/or humic acid material.
Preferably, count in mass ratio, in the water-soluble fertilizer content of the fertilizer of the growth substance containing natural plants be 5~
85%.
Preferably, a great number of elements raw material is selected from urea, ammonium dihydrogen phosphate, potassium sulfate, potassium dihydrogen phosphate and potassium nitrate
In one or more.
Preferably, the trace element materials are selected from ferrous sulfate, zinc sulfate, manganese sulfate, copper sulphate, ammonium molybdate and boric acid
In one or more.
Preferably, the one kind or more of the humic acid material in mineral sources humic acid, biochemical fulvic acid, potassium fulvate
Kind.
Preferably, the water-soluble fertilizer also includes quinhydrones, sodium lignin sulfonate, glutamate and 3,4 dimethyl
Pyrazoles phosphate.
Preferably, count in mass ratio, the water-soluble fertilizer is made up of following composition：It is of the present invention to be given birth to containing natural plants
Fertilizer 10~20%, urea 15~25%, biochemical fulvic acid liquid 10~20%, quinhydrones 1~5%, the lignin sulfonic acid of long material
Sodium 2~8%, the dimethyl pyrazole phosphate 1~5% of glutamate 5~15%, 3,4 and water 5~50%.
Preferably, count in mass ratio, the water-soluble fertilizer is made up of following composition：It is of the present invention to be given birth to containing natural plants
Fertilizer 15%, urea 20%, biochemical fulvic acid liquid 18%, quinhydrones 3%, sodium lignin sulfonate 6%, the chitosan paddy ammonia of long material
The dimethyl pyrazole phosphate 4% of hydrochlorate 10%, 3,4 and water 24%.
Effective status of the water-soluble fertilizer of the growth substance provided by the invention containing natural plants in soil is stable, can promote
Absorption of the crop to nutrient in fertilizer and soil, promote and stimulate plant growth, enhancing crop drought resistance, cold-resistant, Resistant etc. are more
The degeneration-resistant function of kind, while the synthesis of chlorophyll can be promoted, strengthen photosynthesis, promote sucrose accumulation and conversion, article is made in raising
Matter and yield, utilization rate of fertilizer are high.
Compared with prior art, the beneficial effects of the present invention are：
1) preparation technology of the fertilizer of the growth substance provided by the invention containing natural plants, by add calgon and
Sapindoside carries out low temperature classification extraction and using classification zymolysis technique, and fucosidase creatively is applied into sea-tangle
In natural plants growth substance extraction process, Endogenous hormones in sea-tangle, mannitol, alginic acid, alga oligosaccharides etc. are improved
Recovery rate, overcome the destruction of acidolysis, alkaline hydrolysis and high temperature to natural plants growth substance activity, give birth in fertilizer natural plants
The content of long material is greatly improved；
2) preparation is simple for the fertilizer of the growth substance provided by the invention containing natural plants, and production cost is low,
Production technology is easily controllable, is advantageous to large-scale industrialized production；
3) fertilizer of the growth substance provided by the invention containing natural plants, the content of high-activity natural plant growth substance
Height, seed sprouting can be effectively facilitated, increases root activity；Strengthen a variety of degeneration-resistant functions such as crop drought resistance, cold-resistant, Resistant；
Promote the synthesis of chlorophyll, strengthen photosynthesis, promote sucrose accumulation and conversion, improve crop quality and yield；
4) effective status of the water-soluble fertilizer of the growth substance provided by the invention containing natural plants in soil is stable, can promote
Enter absorption of the crop to nutrient in fertilizer and soil, promote and stimulate plant growth, enhancing crop drought resistance, cold-resistant, Resistant etc.
A variety of degeneration-resistant functions, while the synthesis of chlorophyll can be promoted, strengthen photosynthesis, promote sucrose accumulation and conversion, improve crop
Quality and yield, utilization rate of fertilizer are high.
Brief description of the drawings
Sea-tangle cell wall electron microscope before Fig. 1 enzymolysis processings.
Sea-tangle cell wall electron microscope after Fig. 2 enzymolysis processings.
Embodiment
The present invention is further described below by way of embodiment, but the present invention is not limited only to following examples.
In the scope of the present invention or do not departing from present disclosure, in spirit and scope, the present invention is appropriately modified, be equivalent
Change or modification, it will become apparent to those skilled in the art that they are considered as being included in the scope of the present invention
Within.
It is raw materials used in the embodiment of the present invention, unless otherwise specified, it can be obtained by conventional commercial approach, such as：Fiber
Plain enzyme is purchased from Qingdao Ji Baozhongxin International Trading Company Ltd, specification：700EGU/mL；Pectase contains industry collection purchased from the Ningxia summer
Co., Ltd of group, specification：600U/mL；Fucosidase is purchased from Shanghai Zhen Zhun bio tech ltd, specification：10U at 25
℃。
The fertilizer of the growth substance containing natural plants of embodiment 1 and its preparation
The fertilizer of the growth substance containing natural plants of the embodiment of the present invention 1, its preparation technology comprise the following steps：
S1：Dried after sea-tangle impurity elimination is cleaned, crush, add the calgon of its quality 0.5 ‰ and 3 ‰ soapberry
Saponin(e, solid-liquid ratio 1 is pressed after mixing：5g/mL extracts 90min under conditions of adding water to be 25 DEG C in temperature, filters to obtain leaching liquor I；Will
Sea-tangle after filtering presses solid-liquid ratio 1：3g/mL extracts 60min under conditions of adding water to be 25 DEG C in temperature, filters to obtain leaching liquor II,
It is standby to retain kelp residue；Leaching liquor I and leaching liquor II is merged, the 1/3 of original volume is concentrated under reduced pressure into after mixing, it is dense to obtain leaching liquor
Contracting liquid；
S2：Kelp residue is pressed into solid-liquid ratio 1：8g/mL adds water to mix, and the cellulase of kelp residue quality 1.2% is added, in temperature
Spend for 50 DEG C, pH value be 5.8 under conditions of digest 2h；Add the pectase of kelp residue quality 1.5%, temperature be 45 DEG C,
PH value digests 0.75h under conditions of being 3.8；Be eventually adding the fucosidase of kelp residue quality 0.4%, temperature be 45 DEG C,
PH value digests 1.5h under conditions of being 7.0, filters, obtains enzymolysis liquid；
S3：The pH value for adjusting enzymolysis liquid is 7.0, adds and extracts concentrated liquor described in step S1, is concentrated under reduced pressure into after mixing
The 2/3 of original volume, produce the fertilizer of the growth substance containing natural plants of the embodiment of the present invention 1.
The fertilizer of the growth substance containing natural plants of embodiment 2 and its preparation
The fertilizer of the growth substance containing natural plants of the embodiment of the present invention 2, its preparation technology comprise the following steps：
S1：Dried after sea-tangle impurity elimination is cleaned, crush, add the calgon of its quality 2 ‰ and 8 ‰ soapberry soap
Glycosides, solid-liquid ratio 1 is pressed after mixing：4g/mL extracts 60min under conditions of adding water to be 35 DEG C in temperature, filters to obtain leaching liquor I；Incited somebody to action
Sea-tangle after filter presses solid-liquid ratio 1：2g/mL extracts 30min under conditions of adding water to be 35 DEG C in temperature, filters to obtain leaching liquor II, protects
Stay kelp residue standby；Leaching liquor I and leaching liquor II is merged, the 1/5 of original volume is concentrated under reduced pressure into after mixing, obtains leaching liquor concentration
Liquid；
S2：Kelp residue is pressed into solid-liquid ratio 1：12g/mL adds water to mix, and adds the cellulase of kelp residue quality 1.8%,
Temperature is 55 DEG C, pH value digests 1h under conditions of being 6.2；The pectase of kelp residue quality 1.8% is added, is 50 in temperature
DEG C, pH value be 4.5 under conditions of digest 1h；Be eventually adding the fucosidase of kelp residue quality 0.6%, temperature be 55 DEG C,
PH value digests 0.75h under conditions of being 6.0, filters, obtains enzymolysis liquid；
S3：The pH value for adjusting enzymolysis liquid is 7.0, adds and extracts concentrated liquor described in step S1, is concentrated under reduced pressure into after mixing
The 4/5 of original volume, produce the fertilizer of the growth substance containing natural plants of the embodiment of the present invention 2.
The fertilizer of the growth substance containing natural plants of embodiment 3 and its preparation
The fertilizer of the growth substance containing natural plants of the embodiment of the present invention 3, its preparation technology comprise the following steps：
S1：Dried after sea-tangle impurity elimination is cleaned, crush, add the calgon of its quality 1 ‰ and 5 ‰ soapberry soap
Glycosides, solid-liquid ratio is pressed after mixing:1：4g/mL extracts 80min under conditions of adding water to be 30 DEG C in temperature, filters to obtain leaching liquor I；Incited somebody to action
Sea-tangle after filter presses solid-liquid ratio 1：3g/mL extracts 40min under conditions of adding water to be 30 DEG C in temperature, filters to obtain leaching liquor II, protects
Stay kelp residue standby；Leaching liquor I and leaching liquor II is merged, the 1/3 of original volume is concentrated under reduced pressure into after mixing, obtains leaching liquor concentration
Liquid；
S2：Kelp residue is pressed into solid-liquid ratio 1：10g/mL adds water to mix, and adds the cellulase of kelp residue quality 1.5%,
Temperature is 60 DEG C, pH value digests 1.5h under conditions of being 6.0；The pectase of kelp residue quality 1.5% is added, is 60 in temperature
DEG C, pH value be 4.0 under conditions of digest 1h；Be eventually adding the fucosidase of kelp residue quality 0.5%, temperature be 60 DEG C,
PH value digests 1h under conditions of being 6.5, filters, obtains enzymolysis liquid；
S3：The pH value for adjusting enzymolysis liquid is 7.0, adds and extracts concentrated liquor described in step S1, is concentrated under reduced pressure into after mixing
The 2/3 of original volume, produce the fertilizer of the growth substance containing natural plants of the embodiment of the present invention 3.
The water-soluble fertilizer of the growth substance containing natural plants of embodiment 4 and its preparation
The water-soluble fertilizer of the growth substance containing natural plants described in the embodiment of the present invention 4, is counted in mass ratio, by as follows into packet
Into：The fertilizer 85% of the growth substance containing natural plants prepared by the embodiment of the present invention 1, potassium dihydrogen phosphate 15%.
Preparation method：The growth substance containing natural plants of 85% embodiment of the present invention 1 preparation is added in a kettle
Fertilizer and 15% potassium dihydrogen phosphate, are uniformly mixed, and produce the water-soluble fertilizer of the growth substance containing natural plants.
The water-soluble fertilizer of the growth substance containing natural plants of embodiment 5 and its preparation
The water-soluble fertilizer of the growth substance containing natural plants described in the embodiment of the present invention 5, is counted in mass ratio, by as follows into packet
Into：The fertilizer 10% of the growth substance containing natural plants prepared by the embodiment of the present invention 3, urea 23% and biochemical fulvic acid liquid
67%.
Preparation method：The growth substance containing natural plants of 10% embodiment of the present invention 3 preparation is added in a kettle
Fertilizer, 23% urea and 67% biochemical fulvic acid, are uniformly mixed, and produce the Water soluble fertilizer of the growth substance containing natural plants
Material.
The water-soluble fertilizer of the growth substance containing natural plants of embodiment 6 and its preparation
The water-soluble fertilizer of the growth substance containing natural plants described in the embodiment of the present invention 6, is counted in mass ratio, by as follows into packet
Into：The fertilizer 8% of the growth substance containing natural plants prepared by the embodiment of the present invention 2, zinc nitrate 15%, ferrous sulfate 15%, molybdenum
Sour ammonium 1%, boric acid 1%, 100% is supplied with water.
Preparation method：The fertilizer of the growth substance containing natural plants of 8% embodiment of the present invention 2 preparation is added in a kettle
Material, 15% zinc nitrate, 15% ferrous sulfate, 1% ammonium molybdate and 1% boric acid are sequentially added, finally add 60%
Water, it is uniformly mixed, produces the water-soluble fertilizer of the growth substance containing natural plants.
The water-soluble fertilizer of the growth substance containing natural plants of embodiment 7 and its preparation
The water-soluble fertilizer of the growth substance containing natural plants described in the embodiment of the present invention 7, is counted in mass ratio, by as follows into packet
Into：The fertilizer 10% of the growth substance containing natural plants made from the embodiment of the present invention 3, urea 23%, biochemical fulvic acid liquid 20%,
Quinhydrones 1%, sodium lignin sulfonate 2%, the dimethyl pyrazole phosphate 5% of glutamate 5%, 3,4 and water 34%.
Preparation method：The fertilizer of 10% embodiment of the present invention, the 3 obtained growth substance containing natural plants is added in a kettle
Material, sequentially add 23% urea, 20% biochemical fulvic acid liquid, 1% quinhydrones, 2% sodium lignin sulfonate, 5%
Glutamate, the 3 of 5%, 4 dimethyl pyrazole phosphate, finally plus 34% water, be uniformly mixed, produce containing day
The water-soluble fertilizer of right plant growth substance.
The water-soluble fertilizer of the growth substance containing natural plants of embodiment 8 and its preparation
The water-soluble fertilizer of the growth substance containing natural plants described in the embodiment of the present invention 8, is counted in mass ratio, by as follows into packet
Into：The fertilizer 15% of the growth substance containing natural plants made from the embodiment of the present invention 3, urea 20%, biochemical fulvic acid liquid 18%,
Quinhydrones 3%, sodium lignin sulfonate 6%, the dimethyl pyrazole phosphate 4% of glutamate 10%, 3,4 and water 24%.
Preparation method：The growth substance containing natural plants of 15% embodiment of the present invention 3 preparation is added in a kettle
Fertilizer, sequentially add 20% urea, 18% biochemical fulvic acid liquid, 3% quinhydrones, 6% sodium lignin sulfonate, 10%
Glutamate, the 3 of 4%, 4 dimethyl pyrazole phosphate, finally plus 24% water, be uniformly mixed, produce and contain
The water-soluble fertilizer of natural plants growth substance.
The fertilizer of the growth substance containing natural plants of comparative example 1 and its preparation
Compared with Example 3, the fertilizer of this comparative example growth substance containing natural plants differs only in：Preparation technology walks
Suddenly S1 is：Dried after sea-tangle impurity elimination is cleaned, crush, add the calgon of its quality 1 ‰ and 5 ‰ sapindoside,
Solid-liquid ratio is pressed after mixing:1：7g/mL extracts 120min under conditions of adding water to be 30 DEG C in temperature, filters to obtain leaching liquor, retains sea
Band slag is standby；Leaching liquor is concentrated under reduced pressure into the 1/3 of original volume, obtains extraction concentrated liquor.
Remaining step is the same as embodiment 3.
The fertilizer of the growth substance containing natural plants of comparative example 2 and its preparation
Compared with Example 3, the fertilizer of this comparative example growth substance containing natural plants differs only in：Preparation technology walks
Calgon is not added with rapid S1.
Remaining step is the same as embodiment 3.
The fertilizer of the growth substance containing natural plants of comparative example 3 and its preparation
Compared with Example 3, the fertilizer of this comparative example growth substance containing natural plants differs only in：Preparation technology walks
In rapid S2, it is not added with fucosidase and is digested.
Remaining step is the same as embodiment 3.
The water-soluble fertilizer of the growth substance containing natural plants of comparative example 4 and its preparation
Compared with Example 8, the water-soluble fertilizer of this comparative example growth substance containing natural plants differs only in：It is not hydrogeneous
Quinone.
Remaining is the same as embodiment 8.
The water-soluble fertilizer of the growth substance containing natural plants of comparative example 5 and its preparation
Compared with Example 8, the water-soluble fertilizer of this comparative example growth substance containing natural plants differs only in：Without shell
Glycan glutamate.
Remaining is the same as embodiment 8.
The assay of test example one, natural plants growth substance
With embodiment 1-3, the fertilizer for the growth substance containing natural plants that comparative example 1-3 is prepared is test specimen, right
Its alginic acid, alga oligosaccharides, abscisic acid, gibberellin, the basic element of cell division, the content of mannitol and organic matter are measured, and are determined
It the results are shown in Table 1.
The content of each natural plants growth substance in the fertilizer of the growth substance containing natural plants of table 1
As shown in Table 1：
(1) fertilizer of the growth substance provided by the invention containing natural plants contains the abscisic acid, gibberellin, cell of high content
The natural plants growth substance such as mitogen, alginic acid, alga oligosaccharides, mannitol, the activity of improving growth is high, therefore can be effective
Promotion crop growth；
(2) preparation is simple for the fertilizer of the growth substance provided by the invention containing natural plants, overcome acidolysis,
The destruction of alkaline hydrolysis and high temperature to natural plants growth substance activity, can effectively extract and retain the bioactive substance in sea-tangle,
It is thin to sea-tangle before and after enzymolysis processing in the fertilizer preparation process of the growth substance containing natural plants of embodiment 3 with transmission electron microscope
The broken situation of born of the same parents' cell membrane is observed, as a result as depicted in figs. 1 and 2, as shown in Figure 2, sea-tangle cell after enzymolysis processing
Cell membrane is broken complete, thus can make intracellular inclusion discharge and obtain into the cell by macromolecular substances degraded,
It is easy to the small-molecule substance being absorbed and used, improves the content of natural plants growth substance in fertilizer；
(3) comparative example 1 uses a soaking technology, due to solid-liquid ratio is high and soak time length cause abscisic acid, gibberellin,
The basic element of cell division and mannitol isoreactivity composition resorption, natural plants growth substance leaching rate reduce；The fertilizer of comparative example 2 prepares work
In skill, leaching process is not added with calgon, and the component content such as abscisic acid, gibberellin is remarkably decreased in obtained fertilizer；Contrast
Enzymolysis process contains without using fucosidase, the obtained various natural plants growth substances of fertilizer in the fertilizer preparation technology of example 3
Amount significantly reduces, and illustrates that fucosidase can effectively improve the recovery rate of natural plants growth substance in sea-tangle.
Test example two, wheat germination experiment
1st, test specimen：The present invention implements the fertilizer of 3 growth substances containing natural plants, with 900 times of standby of distilled water diluting
With.
2nd, test method：Uniform in size, full seed wheat seed is chosen, test group and blank control group respectively set 4 groups,
80 wheat seeds are one group, and each group wheat seed is soaked seed 4 hours with after 3% disinfectant with hydrogen peroxide, wherein, test group wheat
Seed is soaked seed with the fertilizer of the growth substance containing natural plants of embodiment 3 after 900 times of dilutions, blank control group wheat seed
Soaked seed with distilled water.After seed soaking terminates, wheat seed is put into the culture dish for be covered with filter paper and carries out germination test, tested
Period filter paper keeps soaking with distilled water.Each group is germinateed after the germination percentage and 72h and 96h of each group wheat after record 24h and 48h
Radical, stem length and the root long of wheat seed, average.
3rd, result of the test：It is shown in Table 2 and table 3.
The wheat germination rate of table 2
Radical, stem length and the root long of the germinated wheat seed of table 3
As shown in Table 2, the germination percentage after test group wheat seed 24h is significantly higher than blank control group, illustrates this
The fertilizer of invention growth substance containing natural plants can effectively facilitate quick germination of wheat seeds, improve the germination percentage of wheat seed；
As shown in Table 3, the radical of test group germinated wheat seed is compared with blank control group, no marked difference, but test group
The stem length and root long of germinated wheat seed are considerably longer than blank control group, illustrate the fertilizer of the growth substance of the invention containing natural plants
Material can effectively facilitate the growth of germinated wheat kind blastostyle and root.
In summary, the content of the fertilizer natural plants growth substance of the growth substance of the invention containing natural plants is high, can have
Effect promotes seed to sprout, and increases root activity, promotes growing for crop.
Test example three, corn yield increasing experiment
1st, test specimen：The Water soluble fertilizer of embodiment 8, comparative example 4 and the growth substance containing natural plants made from comparative example 5
Material.
2nd, test method：Test specimen combination Corn Irrigation is applied fertilizer, applies 2kg/ mus, jointing stage in Maize at Seedling Stage respectively
Using 5kg/ mus, the typhon mouth phase applies 8kg/ mus, takes out the male spinning phase and applies 10kg/ mus, the pustulation period applies 5kg/ mus, calculates production
Amount, rate of growth and utilization rate of fertilizer.Positive controls apply the light water of the content of nitrogen and phosphorous identical same amount in the same way
Molten fertilizer.
3rd, result of the test：It is shown in Table 4.
The corn yield increasing result of the test of table 4
| Project | Dose kg/ mus | Corn yield kg/ mus | Rate of growth % | Utilization rate of fertilizer % |
| Conventional water-soluble fertilizer | 30 | 736 | 0 | 28.76 |
| Embodiment 8 | 30 | 987 | 34.10 | 43.54% |
| Comparative example 4 | 30 | 894 | 21.47 | 35.07% |
| Comparative example 5 | 30 | 872 | 18.48 | 33.75% |
As shown in Table 4, water-soluble fertilizer the having in soil of the growth substance provided by the invention containing natural plants
Imitate in stable condition, can promote absorption of the crop to nutrient in fertilizer and soil, promote and stimulate plant growth, enhancing crop resists
A variety of degeneration-resistant functions such as drought, cold-resistant, Resistant, meanwhile, the synthesis of chlorophyll can be promoted, strengthen photosynthesis, promote sugar product
Tired and conversion, improves crop quality and yield, and utilization rate of fertilizer is high.The water-soluble fertilizer of the growth substance containing natural plants of comparative example 4
Without quinhydrones, the water-soluble fertilizer of the growth substance containing natural plants of comparative example 5 is free of glutamate, the volume increase effect to corn
Fruit is not so good as the water-soluble fertilizer of the growth substance containing natural plants of the embodiment of the present invention 8, illustrate answering for quinhydrones and glutamate
With the yield and utilization rate of fertilizer for being advantageous to improve crop.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to is assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (9)
1. the preparation technology of the fertilizer of a kind of growth substance containing natural plants, it is characterised in that comprise the following steps：
S1：By sea-tangle impurity elimination clean after dry, crush, add its quality 0.5~2 ‰ calgon and 3~8 ‰ without trouble
Sub- saponin(e, solid-liquid ratio 1 is pressed after mixing：(3~5) g/mL extracts 60~90min, mistake under conditions of adding water to be 25~35 DEG C in temperature
Filter to obtain leaching liquor I；Sea-tangle after filtering is pressed into solid-liquid ratio 1：(2~4) g/mL soaks under conditions of adding water to be 25~35 DEG C in temperature
30~60min is carried, filters to obtain leaching liquor II, it is standby to retain kelp residue；Leaching liquor I and leaching liquor II is merged, depressurized after mixing
The 1/5~1/3 of original volume is concentrated into, obtains extraction concentrated liquor；
S2：Kelp residue is pressed into solid-liquid ratio 1：(8~12) g/mL adds water to mix, and adds cellulase, is 50~65 DEG C, pH in temperature
It is worth to digest 1~2h under conditions of 5.8~6.5；Add pectase, temperature be 45~60 DEG C, pH value be 3.0~5.0
Under the conditions of digest 0.75~1.5h；Fucosidase is eventually adding, in the condition that temperature is 45~60 DEG C, pH value is 5.5~7.0
0.75~1.5h of lower enzymolysis, filtering, obtains enzymolysis liquid；
S3：The pH value for adjusting enzymolysis liquid is 6.8~7.2, adds and extracts concentrated liquor described in step S1, is concentrated under reduced pressure into after mixing
The 2/3~4/5 of original volume, produce the fertilizer of the growth substance containing natural plants.
2. the preparation technology of the fertilizer of the growth substance containing natural plants as claimed in claim 1, it is characterised in that step S2 institutes
The dosage for stating cellulase is the 1.2~1.8% of kelp residue quality；The dosage of the pectase for kelp residue quality 1.2~
1.8%；The dosage of the fucosidase is the 0.4~0.6% of kelp residue quality.
3. grown as claimed in claim 1 made from the preparation technology of the fertilizer of the growth substance containing natural plants containing natural plants
The fertilizer of material.
4. the water-soluble fertilizer of a kind of growth substance containing natural plants, it is characterised in that the water-soluble fertilizer includes following composition：Power
Profit requires fertilizer, a great number of elements raw material and/or the trace element materials of the growth substance containing natural plants described in 3 and/or rotten plant
Acid starting material.
5. water-soluble fertilizer as claimed in claim 4, it is characterised in that count in mass ratio, claim 3 in the water-soluble fertilizer
The content of the fertilizer of the described growth substance containing natural plants is 5~85%.
6. water-soluble fertilizer as claimed in claim 4, it is characterised in that a great number of elements raw material is selected from urea, biphosphate
One or more in ammonium, potassium sulfate, potassium dihydrogen phosphate and potassium nitrate；The trace element materials are selected from ferrous sulfate, sulfuric acid
One or more in zinc, manganese sulfate, copper sulphate, ammonium molybdate and boric acid；The humic acid material is selected from mineral sources humic acid, life
Change the one or more in fulvic acid, potassium fulvate.
7. water-soluble fertilizer as claimed in claim 4, it is characterised in that the water-soluble fertilizer also includes quinhydrones, lignin sulfonic acid
Sodium, glutamate and 3,4 dimethyl pyrazole phosphate.
8. water-soluble fertilizer as claimed in claim 7, it is characterised in that count in mass ratio, the water-soluble fertilizer is by following composition
Composition：Fertilizer 10~20%, urea 15~25%, the biochemical fulvic acid of the growth substance containing natural plants described in claim 3
Liquid 10~20%, quinhydrones 1~5%, sodium lignin sulfonate 2~8%, glutamate 5~15%, 3,4 dimethyl pyrazoles
Phosphate 1~5% and water 5~50%.
9. water-soluble fertilizer as claimed in claim 8, it is characterised in that count in mass ratio, the water-soluble fertilizer is by following composition
Composition：Fertilizer 15%, urea 20%, biochemical fulvic acid liquid 18%, the hydrogen of the growth substance containing natural plants described in claim 3
Quinone 3%, sodium lignin sulfonate 6%, the dimethyl pyrazole phosphate 4% of glutamate 10%, 3,4 and water 24%.
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| CN108383599A (en) * | 2018-03-30 | 2018-08-10 | 金华市泽雨园艺技术有限公司 | Promote the preparation method of the liquid fertilizer of cuckoo graft survival rate |
| CN109369270A (en) * | 2018-11-26 | 2019-02-22 | 烟台富康生物科技有限公司 | A kind of environment-friendly fertilizer and its preparation process containing functional components of chlorella vulgaris |
| CN110590446A (en) * | 2019-09-25 | 2019-12-20 | 青岛佰蓝海藻有限公司 | Method for preparing liquid seaweed fertilizer by using kelp as raw material |
| CN112028691A (en) * | 2020-09-14 | 2020-12-04 | 成都云图控股股份有限公司 | Instant sterilization type foliar fertilizer and preparation method thereof |
| CN113636892A (en) * | 2021-09-23 | 2021-11-12 | 山东爱果者生物技术有限公司 | Preparation method of seaweed extract and seaweed fertilizer |
| CN114315465A (en) * | 2022-01-13 | 2022-04-12 | 南京阿米巴农业科技研究院有限公司 | Plant growth promoter containing endogenous substances and fertilizer thereof |
| CN114920598B (en) * | 2022-04-19 | 2023-06-06 | 烟台泓源生物肥料有限公司 | Biological fertilizer and preparation method and application thereof |
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