CN106727881A - A kind of extracting method with anti-inflammatory activity general flavone from falcate crazyweed herb - Google Patents
A kind of extracting method with anti-inflammatory activity general flavone from falcate crazyweed herb Download PDFInfo
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- CN106727881A CN106727881A CN201710154119.8A CN201710154119A CN106727881A CN 106727881 A CN106727881 A CN 106727881A CN 201710154119 A CN201710154119 A CN 201710154119A CN 106727881 A CN106727881 A CN 106727881A
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- general flavone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention discloses the extracting method with anti-inflammatory activity general flavone in a kind of falcate crazyweed herb, comprise the following steps：Take falcate crazyweed herb, it is 70% 80% alcohols solvents to add concentration, and Microwave Extraction, the alcohols solvent is 10~35 with the volume mass ratio of medicinal material:1mL/g.The extracting method of general flavone in falcate crazyweed herb of the present invention, it is easy to be quick, can effectively extract the general flavone in falcate crazyweed herb.Meanwhile, present invention also offers application of the extractive of general flavone obtained by foregoing falcate crazyweed herb extracting method in anti-inflammatory drug is prepared.
Description
Technical field
The present invention relates to natural medicine field, and in particular to the extraction side with anti-inflammatory activity general flavone in falcate crazyweed herb
Method.
Background technology
Flavone compound is a series of compounds that two phenyl ring are mutually coupled with by central three carbon phase.Precursor structure
Including including flavonoids, flavanone, flavonols, flavanone alcohols and chalcones etc..
Oxytropis falcate Bunge (Oxytropis falcate Bunge) (the entitled cowherb of Tibetan medicine reaches the summer) is pulse family
(Leguminosae) Genus Oxytropis Dc (Oxytropis D.C.) herbaceos perennial.It is grown in river shoal, the sand of 2700~4300m
Ground, hillside, grassy marshland, mainly originate in Qinghai-Tibet Platean.According to《Jingzhubencao》Record, falcate crazyweed herb have healing the open sore part, it is convenient, prevent
Spur produces, it is ailing to control, except poison, hemostasis the effect of, and can disinfect, treat anthracnose, leprosy and influenza, tonsillitis.
Clinical research proves that falcate crazyweed herb has unique curative effect for prevention, treatment influenza and chronic bronchitis.And
And, pharmacological evaluation proves that falcate crazyweed herb total-flavonoid aglycone is the live part for treating chronic bronchitis, and can increase adrenal gland skin
Matter function, illustrates that flavone compound is the important activity composition of falcate crazyweed herb.
Therefore, the general flavone in falcate crazyweed herb how is effectively extracted, is had great importance.
The content of the invention
To solve the above problems, the invention provides a kind of extracting method of general flavone in falcate crazyweed herb, including following step
Suddenly：
Falcate crazyweed herb is taken, addition concentration is 70%-80% alcohols solvents, Microwave Extraction, the alcohols solvent and medicinal material
Volume mass ratio is 10~35:1mL/g.
Further, the alcohols solvent is methyl alcohol or ethanol.
Further, the concentration of the alcohols solvent is 75%.
Further, the power of the Microwave Extraction is 200W~900W.
Further, the power of the Microwave Extraction is 600W~900W, preferably 700W.
Further, the time of the Microwave Extraction is 10 minutes~40 minutes.
Further, the time of the Microwave Extraction is 20 minutes~40 minutes, preferably 30 minutes.
Further, the alcohols solvent and the volume mass ratio of medicinal material are 20-35:1mL/g, preferably 25:1mL/g.
Further, the Microwave Extraction temperature is 80~95 DEG C, preferably 85 DEG C.
Present invention also offers extractive of general flavone in a kind of falcate crazyweed herb, the extract is to prepare by the following method
Obtain：
(1) falcate crazyweed herb is taken, general flavone extract solution in falcate crazyweed herb is prepared according to foregoing extracting method；
(2) concentrated extracting solution, ceramic membrane filtration, organic film purifying takes filtrate, is drying to obtain.
Present invention also offers application of the foregoing falcate crazyweed herb extractive of general flavone in anti-inflammatory drug is prepared.
Extracting method with anti-inflammatory activity general flavone in falcate crazyweed herb of the present invention, it is easy to be quick, can effectively extract
General flavone in falcate crazyweed herb.Meanwhile, the inflammatory model that the falcate crazyweed herb extract that the present invention is obtained is induced LPS has notable
Improvement result, mainly by suppress inflammatory factor NO, TNF-α etc. discharge, inhibitory action is directly played, so as to show anti-inflammatory
Effect.
Obviously, the above of the invention, according to the ordinary technical knowledge and customary means of this area, is not departing from
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification of other diversified forms can also be made, is replaced or is changed.
The specific embodiment of form, remakes further specifically to the above of the invention by the following examples
It is bright.But this scope for being interpreted as above-mentioned theme of the invention should not be only limitted to following example.It is all based on the above of the present invention
The technology realized belongs to the scope of the present invention.
Specific embodiment
Falcate crazyweed herb picks up from Haibei Prefecture in August, 2015, through Northwest Plateau-organisms Research Inst. of Chinese Academy of Sciences grandson
Cyanines assistant researcher identifies；Rutin standard items are purchased from Chinese drug inspection office (lot number：100080-200707, content is
90.5%), it is pure that ethanol, natrium nitrosum, aluminum nitrate, NaOH are analysis.Self-control ultra-pure water.
Microwave Extraction instrument (RWBC, Nanjing Su Enrui companies) METTLER TOLEDO PL203 and XS204 electronic balances are (auspicious
Scholar Mettler Toledo Inc.)；TU-1901 type ultraviolet-uisible spectrophotometers；
The unit of solid-liquid ratio is ml/g.
Determination of Total Flavonoids method is as follows：
(1) preparation of reference substance solution
Control substance of Rutin 20mg is taken, accurately weighed, in putting 50ml measuring bottles, plus 75% ethanol makes to dissolve and be diluted to scale,
Shake up；Precision measures 25ml, puts in 50ml measuring bottles, is diluted with water to scale, shakes up, and obtains final product (containing rutin 0.2mg in per 1ml).
(2) Specification Curve of Increasing
Precision measures reference substance solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, puts respectively in 25ml measuring bottles, respectively adds 30% second
Alcohol is to 6ml, plus 5% sodium nitrite solution 1ml, mixes, and places 6 minutes, then adds 10% aluminum nitrate solution 1ml, shakes up, and places 6
Minute, plus sodium hydroxide test solution 10ml, then add 30% ethanol to scale, and shake up, place 15 minutes, with corresponding reagent as blank,
According to UV-VIS spectrophotometry (Chinese Pharmacopoeia version four in 2015), the mensuration absorbance at the wavelength of 500nm, with extinction
It is ordinate to spend, and reference substance solution concentration is abscissa, draws standard curve.
(3) preparation of sample solution
The dry falcate crazyweed herb raw materials of 1g accurately are weighed, is placed in 50mL round-bottomed flasks.In selected microwave power, liquid material
Microwave Extraction is carried out than conditions such as, extraction time, Extracting temperature, concentration of alcohol, extract solution is filtered and is settled to 50mL, as sample
Product solution.
(4) general flavone recovery rate is calculated in falcate crazyweed herb
Precision measures need testing solution 2ml, and in putting 25ml measuring bottles, plus 30% ethanol is to 6ml, under sighting target directrix curve preparation
Method, from " plus 5% sodium nitrite solution 1ml ", mensuration absorbance in accordance with the law, while take need testing solution 2ml, except not being hydrogenated with
Outside sodium oxide molybdena test solution, remaining is ibid operated, as blank, the concentration containing rutin from need testing solution is read on standard curve,
Calculated according to following equation, obtained final product.
A (%)=BCm/D × 100
In formula：B is the content (μ g/mL) of rutin in test liquid, and C is final constant volume (mL), and D is extension rate, and m is
Material quality (g).
The extracting method of general flavone in the falcate crazyweed herb of the present invention of embodiment 1
Inventor has carried out the investigation of multiple technological parameters to the extracting method of general flavone in falcate crazyweed herb.
(1) investigation of microwave power
The dry falcate crazyweed herb raw materials of 1g accurately are weighed, is placed in round-bottomed flask, extract 40min, liquid ratio is 20:1, carry
Temperature is taken for 50 DEG C, influence of the different microwave to general flavone recovery rate is investigated, as a result as shown in table 1.
Table 1
| Power (W) | 200 | 300 | 400 | 500 | 600 | 700 | 800 | 900 |
| Recovery rate (%) | 0.57 | 0.68 | 0.77 | 0.82 | 0.96 | 1.15 | 1.08 | 1.01 |
Result shows, in the range of 200W~900W, can effectively extract the flavones in falcate crazyweed herb.Wherein,
Effect is preferable in the range of 600W~900W, 700W best results.
(2) investigation of extraction time
The dry falcate crazyweed herb raw materials of 1g accurately are weighed, is placed in round-bottomed flask, concentration of alcohol is 75%, and Extracting temperature is
50 DEG C, when microwave power is 700W, influence of the different extraction times to general flavone recovery rate is investigated, as a result as shown in table 2.
Table 2
| Time (min) | 10 | 15 | 20 | 30 | 35 | 40 |
| Recovery rate (%) | 0.67 | 0.85 | 0.97 | 1.13 | 1.04 | 1.03 |
Result shows, in the range of 10 minutes~40 minutes, can effectively extract the flavones in falcate crazyweed herb.Its
In, effect is preferable in the range of 20 minutes~40 minutes, 30 minutes best results.
(3) investigation of liquid ratio
The dry falcate crazyweed herb raw materials of 1g accurately are weighed, are placed in round-bottomed flask, microwave power is 700w, extract 35min,
When concentration of alcohol is 75%, influence of the different liquid ratios to general flavone recovery rate is investigated, as a result as shown in table 3.
Table 3
Result shows, 1:10~1:In the range of 35, the flavones in falcate crazyweed herb can be effectively extracted.Wherein, 1:
20~1:Effect is preferable in the range of 35, and 1:25 best results.
(4) investigation of temperature
The dry falcate crazyweed herb raw materials of 1g accurately are weighed, are placed in round-bottomed flask, microwave power is 700w, extract 35min,
When concentration of alcohol is 75%, the influence that different temperatures compares general flavone recovery rate is investigated, as a result as shown in table 4.
Table 4
| Temperature (DEG C) | 70 | 80 | 85 | 90 | 95 |
| Recovery rate (%) | 0.85 | 0.97 | 1.15 | 1.11 | 1.06 |
Result shows, in the range of 70-95 DEG C, can effectively extract the flavones in falcate crazyweed herb.Wherein, 80~95
Effect is preferable in the range of DEG C, 85 DEG C of best results.
On the basis of the above results, inventor has carried out further optimal screening to extraction process, as a result shows, when micro-
To 731W, extraction time is 26 minutes, liquid ratio 26 to wave power：When 1, general flavone recovery rate is in falcate crazyweed herb under the conditions of being somebody's turn to do
1.56%.
The preparation of the falcate crazyweed herb extractive of general flavone of the present invention of embodiment 2
1) Microwave Extraction：Microwave Extraction power is 731w, and extraction time is 26min, and liquid ratio is 26, and 80% methyl alcohol is carried
Take.
2) extract solution concentration.
3) ceramic membrane filter removal of impurities：0.2um ceramic membranes, pressure 1MPa, flow velocity 21ml is per minute, removes fine slag, soil
Deng impurity, reclaim through survey solution.
4) organic membrane separating and purifying：Molecular weight is used to be purified for 2000 molecular weight organic film, pressure is 0.7MPa,
Flow velocity 2ml is per minute, collects through survey solution
5) dry：Microwave drying freeze-drying is spray-dried
6) flavones content>50%.
Concrete composition includes that the double hydrogen of 7- hydroxyl dihydro anthoxanthins, 5,7- -4 '-methoxies of dihydroxy flavonols, 5,7- dihydroxy are yellow
Ketone, 2 ', 4 '-dihydroxy-chalcone, 2 ', 4 '-dihydroxy dihydrochalcone, rutin, robinin, (2R, 3R)-(+)-aromadendrin-
3- β-D-Glucose, FNL, apiolin 7-O- glucose, oxytroflavoside A,
oxytroflavoside B、Coxytroflavoside C、oxytroflavoside F、oxytroflavoside G。
The preparation of the falcate crazyweed herb extract of the present invention of embodiment 3
Preparation method：
1) Microwave Extraction：Microwave Extraction power is 731w, and extraction time is 26min, and liquid ratio is 26, and 75% ethanol is carried
Take.
2) extract solution concentration.
3) ceramic membrane filter removal of impurities：0.1um ceramic membranes, pressure 3MPa, flow velocity 50ml is per minute, removes fine slag, soil
Deng impurity, reclaim through survey solution.
4) organic membrane separating and purifying：Use molecular weight to be purified for 2000 molecular weight organic film, pressure is 1MPa, flow
Fast 50ml is per minute, collects through survey solution.
5) microwave drying, freeze-drying, spray drying are dried.
6) flavones content>50%.
Concrete composition includes that the double hydrogen of 7- hydroxyl dihydro anthoxanthins, 5,7- -4 '-methoxies of dihydroxy flavonols, 5,7- dihydroxy are yellow
Ketone, 2 ', 4 '-dihydroxy-chalcone, 2 ', 4 '-dihydroxy dihydrochalcone, rutin, robinin, (2R, 3R)-(+)-aromadendrin-
3- β-D-Glucose, FNL, apiolin 7-O- glucose, oxytroflavoside A,
oxytroflavoside B、Coxytroflavoside C、oxytroflavoside F、oxytroflavoside G。
The preparation of the falcate crazyweed herb extract of the present invention of embodiment 4
Preparation method：1) Microwave Extraction, Microwave Extraction power is 731w, and extraction time is 26min, and liquid ratio is 26,
75% ethanol is extracted.
2) extract solution concentration
3) ceramic membrane filter removal of impurities：0.2um ceramic membranes, pressure 2MPa, flow velocity 30ml is per minute, removes fine slag, soil
Deng impurity, reclaim through survey solution.
4) organic membrane separating and purifying：Molecular weight is used to be purified for 2000 molecular weight organic film, pressure is 0.8MPa,
Flow velocity 40ml is per minute, collects through survey solution.
5) microwave drying, freeze-drying, spray drying are dried.
6) flavones content>50%.
Concrete composition includes that the double hydrogen of 7- hydroxyl dihydro anthoxanthins, 5,7- -4 '-methoxies of dihydroxy flavonols, 5,7- dihydroxy are yellow
Ketone, 2 ', 4 '-dihydroxy-chalcone, 2 ', 4 '-dihydroxy dihydrochalcone, rutin, robinin, (2R, 3R)-(+)-aromadendrin-
3- β-D-Glucose, FNL, apiolin 7-O- glucose, oxytroflavoside A,
oxytroflavoside B、Coxytroflavoside C、oxytroflavoside F、oxytroflavoside G。
The anti-inflammatory activity of the extract of the present invention of embodiment 5
Anti-inflammatory activity
1) the RAW264.7 cytotoxicities IC50 of mtt assay Detection and Extraction thing>200
2) macrophage RAW264.7 is cultivated, TNF-α is detected respectively, the index of IL-1 β, IL-6, NO, detection method is shown in text
Offer：
1 single sonorous stone side, Wang Qiangsong, Cui Yuanlu, Biqi capsules extract to the antiinflammatory action of RAW264.7 cell models, in
Patent medicine, 2014,36 (1)：26-30.
2 Wang Juan, Jiang Wei, Wang Yi, flavone compound is to lipopolysaccharide-induced RAW264.7 cell antiinflammatory actions in dalbergia wood
Research, cell and molecular immunology magazine, 2013,29 (7)：681-684.
3 stone Meng Qiong, Qin Huilin, Zhang Yongfeng, Liu Ying, Deng Wei, Yang Wenyan, etc., pawpaw triterpene under lipopolysaccharide-induced
The influence of the RAW264.7 cellular inflammation model cell factors, Pharmacology and Clinics of Chinese Materia Medica, 2016,32 (3)：76-80.
The 4 Luo Fu tinkling of pieces of jade, Zhao Hengguang, Li Hongzhong, Wan Jingyuan, Zhou Qixin, Tet is thin to lipopolysaccharide-induced lower RAW264.7
The effect of born of the same parents' inflammatory model cell factor, Chinese herbal medicine, 2011,42 (3):542-545.
3) testing result
As can be seen that the inflammatory model tool that the falcate crazyweed herb extract that the present invention is obtained is induced LPS is significantly improved
Effect, is mainly discharged by suppressing inflammatory factor NO, TNF-α etc., inhibitory action is directly played, so as to show antiinflammatory action.
Claims (10)
1. there is the extracting method of anti-inflammatory activity general flavone in falcate crazyweed herb, it is characterised in that：Comprise the following steps：
Take falcate crazyweed herb, addition concentration is 70%-80% alcohols solvents, Microwave Extraction, the volume of the alcohols solvent and medicinal material
Mass ratio is 10~35:1mL/g.
2. extracting method according to claim 1, it is characterised in that：The lower alcohols solvent is methyl alcohol or ethanol.
3. extracting method according to claim 1, it is characterised in that：The concentration of the alcohols solvent is 75%.
4. extracting method according to claim 1, it is characterised in that：The power of the Microwave Extraction is 200W~900W,
It is preferred that 600W~900W, more preferably 700W.
5. the extracting method according to claim any one of 1-4, it is characterised in that：The time of the Microwave Extraction is 10 points
Clock~40 minute, more preferably preferably 20 minutes~40 minutes, 30 minutes.
6. according to the extracting method described in claim 1-5 mono-, it is characterised in that：The volume matter of the alcohols solvent and medicinal material
Amount is than being 20-35:1mL/g, preferably 25:1mL/g.
7. the extracting method according to claim any one of 1-3, it is characterised in that：The power of the Microwave Extraction is
731W, the time of Microwave Extraction is 26 minutes, and alcohols solvent is 26 with the volume mass ratio of medicinal material:1mL/g.
8. the extracting method according to claim any one of 1-3, it is characterised in that：The Microwave Extraction temperature is 80~95
DEG C, preferably 85 DEG C.
9. a kind of falcate crazyweed herb extractive of general flavone, it is characterised in that：The extract is prepared by the following method：
(1) falcate crazyweed herb is taken, general flavone in falcate crazyweed herb is prepared according to the extracting method described in claim any one of 1-8
Extract solution；
(2) concentrated extracting solution, ceramic membrane filtration, organic film purifying takes filtrate, is drying to obtain.
10. application of the falcate crazyweed herb extractive of general flavone in anti-inflammatory drug is prepared described in claim 9.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108659073A (en) * | 2018-04-28 | 2018-10-16 | 中国科学院西北高原生物研究所 | A kind of method and application thereof preparing six kinds of compounds of oxytroflavoside from falcate crazyweed herb |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0578384A (en) * | 1991-03-27 | 1993-03-30 | Taisho Pharmaceut Co Ltd | Flavonol derivative |
| CN104069155A (en) * | 2014-06-27 | 2014-10-01 | 塔里木大学 | Application of oxytropis glabra flavonoid extract taken as bacteriostatic agent |
| CN104435387A (en) * | 2014-11-27 | 2015-03-25 | 普正药业股份有限公司 | Method for extracting total flavonoids of callicarpa nudiflora |
| CN105079085A (en) * | 2015-09-10 | 2015-11-25 | 甘肃省中医药研究院 | Extraction process of total flavonoids of oxytropis falcata |
| CN105481921A (en) * | 2015-12-29 | 2016-04-13 | 中国科学院西北高原生物研究所 | Method for separating new compound from whin based on parallel separation type preparative chromatography |
-
2017
- 2017-03-15 CN CN201710154119.8A patent/CN106727881A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0578384A (en) * | 1991-03-27 | 1993-03-30 | Taisho Pharmaceut Co Ltd | Flavonol derivative |
| CN104069155A (en) * | 2014-06-27 | 2014-10-01 | 塔里木大学 | Application of oxytropis glabra flavonoid extract taken as bacteriostatic agent |
| CN104435387A (en) * | 2014-11-27 | 2015-03-25 | 普正药业股份有限公司 | Method for extracting total flavonoids of callicarpa nudiflora |
| CN105079085A (en) * | 2015-09-10 | 2015-11-25 | 甘肃省中医药研究院 | Extraction process of total flavonoids of oxytropis falcata |
| CN105481921A (en) * | 2015-12-29 | 2016-04-13 | 中国科学院西北高原生物研究所 | Method for separating new compound from whin based on parallel separation type preparative chromatography |
Non-Patent Citations (5)
| Title |
|---|
| SHAN-SHAN WANG等: "3-Hydroxy-3-Methylglutaryl Flavonol Glycosides from Oxytropis", 《JOURNAL OF NATURAL PRODUCTS》 * |
| 卢建 等: "《新编病理生理学》", 31 December 2011, 中国协和医科大学出版社 * |
| 李茂星等: "藏药镰形棘豆的研究进展", 《中药材》 * |
| 杨欢: "藏药镰形棘豆的抗炎活性及化学成分研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
| 王栋: "藏药镰形棘豆黄酮类成分抗炎与抗氧化活性研究及其含量测定", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108659073A (en) * | 2018-04-28 | 2018-10-16 | 中国科学院西北高原生物研究所 | A kind of method and application thereof preparing six kinds of compounds of oxytroflavoside from falcate crazyweed herb |
| CN108659073B (en) * | 2018-04-28 | 2021-03-23 | 中国科学院西北高原生物研究所 | Method for preparing oxytrofloxacin six compounds from Oxytropis falcate and application thereof |
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Application publication date: 20170531 |