CN106721511A - A kind of grass carp Chinese herbal feed additive and preparation method thereof - Google Patents
A kind of grass carp Chinese herbal feed additive and preparation method thereof Download PDFInfo
- Publication number
- CN106721511A CN106721511A CN201611032211.9A CN201611032211A CN106721511A CN 106721511 A CN106721511 A CN 106721511A CN 201611032211 A CN201611032211 A CN 201611032211A CN 106721511 A CN106721511 A CN 106721511A
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- Prior art keywords
- parts
- grass carp
- feed additive
- chinese herbal
- herbal feed
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- Pending
Links
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Classifications
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Landscapes
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- Fodder In General (AREA)
Abstract
The invention belongs to feed addictive research and development technology field, more particularly to a kind of grass carp Chinese herbal feed additive and preparation method thereof.A kind of grass carp Chinese herbal feed additive, by being made up of following components by weight:10 20 parts of 10 30 parts of Radix Angelicae Sinensis, 10 20 parts of baked ginger, 8 20 parts of the fruit of Chinese magnoliavine, 10 30 parts of Psoralea corylifolia, 7 21 parts of chrysanthemum, windproof 15 30 parts, 10 19 parts of asarum, 8 15 parts of stiff silkworm, 9 20 parts of ramulus mori, 25 35 parts of radix bupleuri, 9 16 parts of balloonflower root, 10 25 parts of the dried immature fruit of citron orange and plantain seed.There is synergistic function between each traditional Chinese medicine ingredients of grass carp Chinese herbal feed additive of the invention, it is ill that the Chinese herbal feed additive prepared is prevented from grass carp, grass carp survival rate is improved, improves lysozyme, superoxide dismutase, catalase, the activity level of alkaline phosphatase in grass carp body.
Description
Technical field
The invention belongs to feed addictive research and development technology field, more particularly to a kind of grass carp Chinese herbal feed additive and its system
Preparation Method.
Background technology
Grass carp is one of large Chinese carp of China four, is also the main Freshwater Fish of China, grass carp cultivation have feed cost it is low,
The advantages of economic benefit is obvious.But due to red skin disease, gill rot, enteritis disease and bleeding that grass carp is easily subject in breeding process
Disease is referred to as the infringement of " disease of grass carp four ", and fish production is made a big impact.Problem is prevented and treated in order to solve grass carp " four disease ",
Need the immunity function of raising grass carp conscientiously.
Chinese herbal medicine is a kind of preferably natural, environment-friendly medicine, small, safe, residual with efficient, toxic and side effect
Many advantages, such as staying few, and wide material sources, low cost.The immunity for improving aquatic livestock is applied by Chinese herbal medicine, fish is improved
Class premunition, the incidence of disease for reducing aquatic livestock is the focus studied in recent years.In comprehensive new shape for advocating healthy aquaculture at present
Under gesture, fish immunity function is improved using Chinese herbal medicine, be extremely important.
Application No. 201310630290.3《Immune formulation for improving grass carp immunity function》Patent of invention it is public
Open a kind of immune formulation for improving grass carp immunity function, it is characterised in that:Bulk drug is by umbellate pore furgus, wilsonii, the bighead atractylodes rhizome, people
Ginseng leaf, cordate houttuynia, the bark of eucommia, eclipta, the rhizome of davallia, cassia twig, Radix Isatidis, hawthorn, the membrane of a chicken's gizzard, Chinese trumpet creeper, Chinese yam, reticulate millettia, soap
Angle thorn, Fructus Corni, peach branch, frutus cnidii, date, the tuber of stemona, Gorgon fruit, Galanga Galangal Seed, betel nut, pittosporum illicioides and Horseweed Herb are constituted.From entirety
See, the formula biases toward tonifying spleen and supports kidney, regulation functions of intestines and stomach, and for enteritis disease effect still, but the patent is not given enough
Data illustrate that this kind of immune formulation has ideal effect for the preventing and treating of grass carp viral disease, bacteriosis.
In view of the defect of prior art, a kind of Chinese medicine fodder addition for improving grass carp immunity function of present invention proposition
Agent, its have efficiently, have no toxic side effect, safe, noresidue many advantages, such as, and wide material sources, low cost, to fish
Immune system carries out across-the-board regulation, the dynamic resistance against diseases of aquatic products and immunity function is improved, so that preventing and curing of fish disease.
The information for being disclosed in the background section is merely intended to increase the understanding to general background of the invention, without answering
In being considered as recognizing or imply in any form that the information structure has been the prior art well known to persons skilled in the art.
The content of the invention
For above shortcomings in the prior art, the present invention provides a kind of grass carp Chinese herbal feed additive and its preparation
Method, the Chinese herbal feed additive reasonable recipe, prepare simple, effect substantially, have no toxic side effect, have no drug resistance it is residual with medicine
Stay, grass carp immunity function can be effectively improved.
To achieve these goals, the present invention is realized using following technical scheme:
A kind of grass carp Chinese herbal feed additive, by being made up of following components by weight:Radix Angelicae Sinensis 10-30 parts, baked ginger 10-20
Part, fruit of Chinese magnoliavine 8-20 parts, Psoralea corylifolia 10-30 parts, chrysanthemum 7-21 parts, it is windproof 15-30 parts, asarum 10-19 parts, stiff silkworm 8-15 parts,
Ramulus mori 9-20 parts, radix bupleuri 25-35 parts, balloonflower root 9-16 parts, dried immature fruit of citron orange 10-25 parts and plantain seed 10-20 parts.
Preferably, described grass carp Chinese herbal feed additive, by being made up of following components by weight:20 parts of Radix Angelicae Sinensis,
15 parts of baked ginger, 14 parts of the fruit of Chinese magnoliavine, 25 parts of Psoralea corylifolia, 12 parts of chrysanthemum, windproof 20 parts, 15 parts of asarum, 11 parts of stiff silkworm, 15 parts of ramulus mori,
15 parts of 30 parts of radix bupleuri, 12 parts of balloonflower root, 20 parts of the dried immature fruit of citron orange and plantain seed.
In addition, present invention also offers the preparation method of described grass carp Chinese herbal feed additive, comprising the following steps:
(1) by weight weigh formula in Radix Angelicae Sinensis, baked ginger, the fruit of Chinese magnoliavine, Psoralea corylifolia, chrysanthemum, windproof, asarum, stiff silkworm,
Ramulus mori, radix bupleuri, balloonflower root, the dried immature fruit of citron orange, plantain seed, clean and dry, and in addition to asarum, after being pulverized and mixed by pulverizer, add its 3-4 times
The running water of weight, first boils again slow fire by intense fire and endures 0.5-1h, and first filtrate is obtained after filtering, and 2-3 times of its weight is added toward the dregs of a decoction
Running water, boil rear slow fire by intense fire and endure 0.5h and add that asarum is continuous to endure 10-15min, pour out second filtrate, merging is filtered twice
Chinese medicine liquid study is concentrated to give after liquid;
(2) to the absolute ethyl alcohol that 3-5 times of its volume is added in the Chinese medicine liquid study obtained by step (1), stirring and dissolving dilution,
Flow back 2.5-3.5h at a temperature of 85-95 DEG C, obtains refined Chinese medicine liquid;
(3) the refined Chinese medicine liquid obtained by step (2) is taken, after ethanol is recovered under reduced pressure, by Chinese medicine liquid low-temperature evaporation, latex shape is obtained
Middle medicinal substances, glue milky substance are put into drying machine and are dried, and EAT is 130-150 DEG C, and leaving air temp is 75-90 DEG C,
Powdered rubber is obtained, powdered rubber is crushed to 120 mesh, obtain herb powder;
(4) herb powder obtained by step (3) is admixed the corn flour of 2-3 times of its quality, is mixed, carry out expanded, temperature
At 85-90 DEG C, puffing time 1-2min, then granulation, cooling treats that particulate component is cooled to 20-25 DEG C, and packaging is obtained final product for control
Grass carp Chinese herbal feed additive.
Compared with prior art, the present invention has the advantages that:
(1) there is synergistic function, in preparing between each traditional Chinese medicine ingredients of grass carp Chinese herbal feed additive of the invention
Medicine feed addictive has functions that convergence removing toxic substances, invigorating the spleen support kidney, promote digestion, and the immune system to fish is adjusted, and improves
The anti-stress ability and immunity function of grass carp, are prevented from grass carp illness, improve grass carp survival rate, improve bacteriolyze in grass carp body
Enzyme, superoxide dismutase, catalase, the activity level of alkaline phosphatase.
(2) grass carp Chinese herbal feed additive raw material of the invention is pure natural raw material, without any chemical classes medicine, system
Preparation Method is simple, and the Chinese herbal feed additive residual rate is low, toxicity is low, and the quality of grass carp is not influenceed, is not detrimental to health.
(3) grass carp Chinese herbal feed additive preparation method of the invention is simple, it is easy to large-scale promotion and application.
Specific embodiment
Specific embodiment of the invention is described in detail with reference to specific embodiment, it is to be understood that of the invention
Protection domain do not limited by specific embodiment.
Embodiment 1:
A kind of grass carp Chinese herbal feed additive, by being made up of following components by weight:10 parts of Radix Angelicae Sinensis, 10 parts of baked ginger, five
8 parts of taste, 10 parts of Psoralea corylifolia, 7 parts of chrysanthemum, windproof 15 parts, 10 parts of asarum, 8 parts of stiff silkworm, 9 parts of ramulus mori, 25 parts of radix bupleuri, balloonflower root 9
10 parts of part, 10 parts of the dried immature fruit of citron orange and plantain seed.
The preparation method of described grass carp Chinese herbal feed additive, comprises the following steps:
(1) by weight weigh formula in Radix Angelicae Sinensis, baked ginger, the fruit of Chinese magnoliavine, Psoralea corylifolia, chrysanthemum, windproof, asarum, stiff silkworm,
Ramulus mori, radix bupleuri, balloonflower root, the dried immature fruit of citron orange and plantain seed, clean and dry, and in addition to asarum, after being pulverized and mixed by pulverizer, add its 3 times
The running water of weight, first boils again slow fire by intense fire and endures 0.5h, and first filtrate is obtained after filtering, and oneself of 2 times of its weight is added toward the dregs of a decoction
Water, boils rear slow fire by intense fire and endures 0.5h and add that asarum is continuous to endure 10min, pours out second filtrate, merges dense after filtrate twice
Contract to obtain Chinese medicine liquid study;
(2) to the absolute ethyl alcohol that 3 times of its volume is added in the Chinese medicine liquid study obtained by step (1), stirring and dissolving dilution,
Flow back 2.5h at a temperature of 85 DEG C, obtains refined Chinese medicine liquid;
(3) the refined Chinese medicine liquid obtained by step (2) is taken, after ethanol is recovered under reduced pressure, by Chinese medicine liquid low-temperature evaporation, latex shape is obtained
Middle medicinal substances, glue milky substance are put into drying machine and are dried, and EAT is 130 DEG C, and leaving air temp is 75 DEG C, obtains powder
Material, 120 mesh are crushed to by powdered rubber, obtain herb powder;
(4) herb powder obtained by step (3) is admixed the corn flour of 2 times of its quality, is mixed, carry out expanded, temperature control
At 85 DEG C, puffing time 1min, then granulation, cooling treats that particulate component is cooled to 20 DEG C to system, and packaging obtains final product the feeding of grass carp Chinese medicine
Feed additives.
Embodiment 2:
A kind of grass carp Chinese herbal feed additive, by being made up of following components by weight:30 parts of Radix Angelicae Sinensis, 20 parts of baked ginger, five
20 parts of taste, 30 parts of Psoralea corylifolia, 21 parts of chrysanthemum, windproof 30 parts, 19 parts of asarum, 15 parts of stiff silkworm, 20 parts of ramulus mori, 35 parts of radix bupleuri, tangerine
20 parts of 16 parts of stalk, 25 parts of the dried immature fruit of citron orange and plantain seed.
The preparation method of described grass carp Chinese herbal feed additive, comprises the following steps:
(1) by weight weigh formula in Radix Angelicae Sinensis, baked ginger, the fruit of Chinese magnoliavine, Psoralea corylifolia, chrysanthemum, windproof, asarum, stiff silkworm,
Ramulus mori, radix bupleuri, balloonflower root, the dried immature fruit of citron orange and plantain seed, clean and dry, and in addition to asarum, after being pulverized and mixed by pulverizer, add its 4 times
The running water of weight, first boils again slow fire by intense fire and endures 1h, and first filtrate is obtained after filtering, and 3 times of its weight is added originally toward the dregs of a decoction
Water, boils rear slow fire by intense fire and endures 0.5h and add that asarum is continuous to endure 15min, pours out second filtrate, is concentrated after merging filtrate twice
Obtain Chinese medicine liquid study;
(2) to the absolute ethyl alcohol that 5 times of its volume is added in the Chinese medicine liquid study obtained by step (1), stirring and dissolving dilution,
Flow back 3.5h at a temperature of 95 DEG C, obtains refined Chinese medicine liquid;
(3) the refined Chinese medicine liquid obtained by step (2) is taken, after ethanol is recovered under reduced pressure, by Chinese medicine liquid low-temperature evaporation, latex shape is obtained
Middle medicinal substances, glue milky substance are put into drying machine and are dried, and EAT is 150 DEG C, and leaving air temp is 90 DEG C, obtains powder
Material, 120 mesh are crushed to by powdered rubber, obtain herb powder;
(4) herb powder obtained by step (3) is admixed the corn flour of 3 times of its quality, is mixed, carry out expanded, temperature control
At 90 DEG C, puffing time 2min, then granulation, cooling treats that particulate component is cooled to 25 DEG C to system, and packaging obtains final product the feeding of grass carp Chinese medicine
Feed additives.
Embodiment 3:
A kind of grass carp Chinese herbal feed additive, by being made up of following components by weight:20 parts of Radix Angelicae Sinensis, 15 parts of baked ginger, five
12 parts of taste, 20 parts of Psoralea corylifolia, 14 parts of chrysanthemum, windproof 20 parts, 15 parts of asarum, 12 parts of stiff silkworm, 12 parts of ramulus mori, 30 parts of radix bupleuri, tangerine
18 parts of 13 parts of stalk, 20 parts of the dried immature fruit of citron orange and plantain seed.
The preparation method of described grass carp Chinese herbal feed additive, comprises the following steps:
(1) by weight weigh formula in Radix Angelicae Sinensis, baked ginger, the fruit of Chinese magnoliavine, Psoralea corylifolia, chrysanthemum, windproof, asarum, stiff silkworm,
Ramulus mori, radix bupleuri, balloonflower root, the dried immature fruit of citron orange, plantain seed, clean and dry, and in addition to asarum, after being pulverized and mixed by pulverizer, add its 3.5 times
The running water of weight, first boils again slow fire by intense fire and endures 0.8h, and first filtrate is obtained after filtering, and 2.5 times of its weight is added toward the dregs of a decoction
Running water, boils rear slow fire by intense fire and endures 0.5h and add that asarum is continuous to endure 12min, second filtrate is poured out, after merging filtrate twice
It is concentrated to give Chinese medicine liquid study;
(2) to the absolute ethyl alcohol that 4 times of its volume is added in the Chinese medicine liquid study obtained by step (1), stirring and dissolving dilution,
Flow back 3.0h at a temperature of 90 DEG C, obtains refined Chinese medicine liquid;
(3) the refined Chinese medicine liquid obtained by step (2) is taken, after ethanol is recovered under reduced pressure, by Chinese medicine liquid low-temperature evaporation, latex shape is obtained
Middle medicinal substances, glue milky substance are put into drying machine and are dried, and EAT is 140 DEG C, and leaving air temp is 80 DEG C, obtains powder
Material, 120 mesh are crushed to by powdered rubber, obtain herb powder;
(4) herb powder obtained by step (3) is admixed the corn flour of 2.5 times of its quality, is mixed, carry out expanded, temperature
At 88 DEG C, puffing time 1-2min, then granulation, cooling treats that particulate component is cooled to 22 DEG C, packaging for control, in obtaining final product grass carp
Medicine feed addictive.
Embodiment 4:Grass carp Chinese herbal feed additive of the invention is tested survival rate and resistance against diseases
Experimental animal:Experiment is purchased from Nanning City fish farm with grass carp, is juvenile fish, body weight 80-86g/ tails, selected experiment fry
Number is 4000 tails, and health is strong, and male and female have.4000 tail grass carps are randomly divided into four groups:Test group 1-3 and control group, every group
Contain the tail of juvenile fish 1000.
Test feed:Chinese herbal feed additive mixed fodder prepared by test group 1-3 difference feeding embodiment of the present invention 1-3
(will 150g above-described embodiments 1-3 prepare Chinese herbal feed additive, 80g soya-bean cakes, 350g wheat flours, 35g crab shell powders, 60g shrimps
Powder, 10g salt, 160g corn flour, 30g fish oil, 15g glycine betaines, 2g calcium dihydrogen phosphates, 2g ironic citrates, 40g B B-complexes
B, 30g yeast, 20g threonines, 20g lysines, are well mixed);The feed of control group feeding is with the difference of test group:Make
The Chinese herbal feed additive for replacing the present invention to use with flavomycoin 20mg/kg.
Feeding method:Date of test is 8 days-October 22 May in 2015.Grass carp is cultivated in fish pond, stocking density 1
Bar/20L.Fish pond area 1500m2(50m long × 30m wide), deep 80cm, with bamboo fence, (water can pass through, and fish can not be by being mark
It is accurate) separate and block into 375m24 blockages;Fish pond coolant-temperature gage whole average 16 DEG C (wherein 24-29 DEG C of summer, average 25.5
DEG C), pH value 6.5 ± 0.2, water quality meets fishery standard.
Feed day feeding volume by fry weight 3-5% it is quantitative it is of fixed place and time feed, 2 times/d is fed, every 10d dosages 1 time.Adjust
Look into the survival rate of grass carp.
Fish is put to death, with buffer solution (pH value 7.5,0.25mol/ sucrose, 0.025mol/L Tris-HCL,
0.1mmol/L EDTA-2Na solution) rinse, then surface moisture is sucked with blotting paper, and remove bone parts, leave
The tissue homogenate such as heart, liver and sexual gland.It is accurate to weigh tissue 0.30g, add 9 times of buffer solution ice bath homogenate, 3000r/min
Low-temperature and high-speed centrifuge 10-15min, takes supernatant 2mL and determines lysozyme and superoxide dismutase vigor.
Test data represents that two sample averages compare to be checked with t, and two samples constitute ratio using means standard deviation (x ± s)
With chi-square criterion, P<0.05 has conspicuousness for difference.
As a result:Influence of the Chinese herbal feed additive to grass carp survival rate, is shown in Table 1;Chinese herbal feed additive to lysozyme and
The influence of superoxide dismutase vigor, the results are shown in Table 2.
Influence of the grass carp Chinese herbal feed additive of the invention of table 1 to grass carp survival rate
Group | Survival number (tail) | Death toll (tail) | Survival rate (%) |
Test group 1 | 990 | 10 | 99.0 |
Test group 2 | 987 | 13 | 98.7 |
Test group 3 | 985 | 15 | 98.5 |
Control group | 835 | 165 | 83.5 |
As it can be seen from table 1 the Chinese herbal feed additive feeding grass carp prepared using the present invention, its ill possibility drop
Low, survival rate is significantly higher than control group.
The grass carp Chinese herbal feed additive of the invention of table 2 is to lysozyme, superoxide dismutase, catalase, alkalescence
The influence of Phosphatase Activity
From table 2 it can be seen that using the grass carp of Chinese herbal feed additive feeding of the present invention relative to control group, internal bacteriolyze
Enzyme, superoxide dismutase, catalase, alkaline phosphatase levels are significantly improved, so as to prove Chinese medicine fodder of the invention
Additive has preferably defence disease, antiviral and antibacterial ability, can significantly improve the immunity function of grass carp.
The foregoing description to specific illustrative embodiment of the invention be in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explaining that certain principles of the invention and its reality should
With so that those skilled in the art can realize and using a variety of exemplaries of the invention and
A variety of selections and change.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (3)
1. a kind of grass carp Chinese herbal feed additive, it is characterised in that by being made up of following components by weight:Radix Angelicae Sinensis 10-30 parts,
Baked ginger 10-20 parts, fruit of Chinese magnoliavine 8-20 parts, Psoralea corylifolia 10-30 parts, chrysanthemum 7-21 parts, windproof 15-30 parts, asarum 10-19 parts, mulberry
9-20 parts, radix bupleuri 25-35 parts, balloonflower root 9-16 parts, dried immature fruit of citron orange 10-25 parts and plantain seed 10-20 parts of branch.
2. grass carp Chinese herbal feed additive according to claim 1, it is characterised in that by by weight by following components system
Into:20 parts of Radix Angelicae Sinensis, 15 parts of baked ginger, 14 parts of the fruit of Chinese magnoliavine, 25 parts of Psoralea corylifolia, 15 parts, 12 parts of chrysanthemum, windproof 20 parts, it is 15 parts of asarum, stiff
15 parts of 11 parts of silkworm, 15 parts of ramulus mori, 30 parts of radix bupleuri, 12 parts of balloonflower root, 20 parts of the dried immature fruit of citron orange and plantain seed.
3. the preparation method of grass carp Chinese herbal feed additive according to claim 1 and 2, it is characterised in that including following
Step:
(1) Radix Angelicae Sinensis, baked ginger, the fruit of Chinese magnoliavine, Psoralea corylifolia, chrysanthemum, windproof, asarum, stiff silkworm, the mulberry in formula are weighed by weight
Branch, radix bupleuri, balloonflower root, the dried immature fruit of citron orange, plantain seed, clean dry, except asarum, in addition to, after being pulverized and mixed by pulverizer, add its 3-4 times
The running water of weight, first boils again slow fire by intense fire and endures 0.5-1h, and first filtrate is obtained after filtering, and 2-3 times of its weight is added toward the dregs of a decoction
Running water, boil rear slow fire by intense fire and endure 0.5h and add that asarum is continuous to endure 10-15min, pour out second filtrate, merging is filtered twice
Chinese medicine liquid study is concentrated to give after liquid;
(2) to the absolute ethyl alcohol that 3-5 times of its volume is added in the Chinese medicine liquid study obtained by step (1), stirring and dissolving dilution,
Flow back 2.5-3.5h at a temperature of 85-95 DEG C, obtains refined Chinese medicine liquid;
(3) the refined Chinese medicine liquid obtained by step (2) is taken, after ethanol is recovered under reduced pressure, by Chinese medicine liquid low-temperature evaporation, latex shape Chinese medicine is obtained
Material, glue milky substance is put into drying machine and is dried, and EAT is 130-150 DEG C, and leaving air temp is 75-90 DEG C, is obtained
Powdered rubber, 120 mesh are crushed to by powdered rubber, obtain herb powder;
(4) herb powder obtained by step (3) is admixed the corn flour of 2-3 times of its quality, is mixed, carry out expanded, temperature control
At 85-90 DEG C, puffing time 1-2min, then granulation, cooling treats that particulate component is cooled to 20-25 DEG C, and packaging obtains final product grass carp
Chinese herbal feed additive.
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