CN106718853A - High-efficiency rice breeding method - Google Patents
High-efficiency rice breeding method Download PDFInfo
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- CN106718853A CN106718853A CN201710170128.6A CN201710170128A CN106718853A CN 106718853 A CN106718853 A CN 106718853A CN 201710170128 A CN201710170128 A CN 201710170128A CN 106718853 A CN106718853 A CN 106718853A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention belongs to rice breeding field, a kind of high-efficiency rice breeding method is specifically disclosed.The method uses the F1 that No. two/an aromatic plant metioned in ancient books of Panjin excellent 822 is obtained for maternal, with 46/ liang of excellent 2161 F1 ' for obtaining of imperial round-grained rice as male parent, prepares combination, reaches the purpose for improveing existing kind Comprehensive Traits.The mutual supplement with each other's advantages of kind is made full use of, from advantages and the close material of other shapes as parent so as to accelerate offspring's stabilized speed.Also, the advanced breeding technique being combined with greenhouse adding generation using molecular marker assisted selection, effectively shortens breeding time, improves efficiency of selection.New lines have high yield, disease resistance, lodging resistance high, and the characteristics of setting percentage (setting percentage is more than 92%) high, drought resisting, suit large area to popularize plantation, can reach cost-saving synergistic, promote the purpose of increasing peasant income.
Description
Technical field
The invention belongs to plant variety breeding technique field, and in particular to a kind of high-efficiency rice breeding method.
Background technology
Rice is one of topmost cereal crops of China, is ploughed with population built-up contract with the improvement of people ' s living standards
The trend for subtracting continues, and improves rice and makees yield, increases stable supplying, is the class that China's rice breeding makes great efforts tackling key problem always for a long time
Topic.It is to improve rice to make the most effective and most economical approach of yield to carry out high and stable yields breeding using excellent germ plasm resource.
But traditional breeding method is carried out certainly again by selection with excellent proterties male parent and maternal advanced hybridization
Hand over, although can also obtain excellent offspring, but the diversity of offspring, multiple screening and cultivation is carried out to offspring, cause
Seed selection process very complicated, cycle be long, efficiency is low.
Therefore, breeding cycle how is shortened, and energy quickly breeding goes out the target product that high yield, comprehensive resistance are strong, setting percentage is high
System is problem demanding prompt solution.
The content of the invention
The technical problems to be solved by the invention are to provide that a kind of high yield, comprehensive resistance are strong, setting percentage is high, breeding cycle is short
Rice breeding method.
In order to solve the above technical problems, the present invention is adopted the following technical scheme that：The high-efficiency rice breeding method, including with
Lower step：
(1) Panjin two and an aromatic plant metioned in ancient books excellent 822 human configuration cross combination of high yield of the selection with high yield multiresistance, obtain F0 generations
Seed, F1 generation seed is obtained by the F0 of acquisition for seed greenhouse adding generation is mixed, goes out to contain high yield by molecular labeling assisting sifting
The F1 generation seed of multiresistance gene；
(2) the anti-fall imperial round-grained rice 46 of selection high yield and high quality and drought-enduring two excellent 2161 human configuration cross combinations, obtain F0 ' generations
Seed, the F0 ' for obtaining is gone out containing high for seed for the mixed F1 ' that obtains of seed greenhouse adding generation by molecular labeling assisting sifting
The F1 ' of the anti-fall Drought-tolerant gene of high-quality is produced for seed；
(3) paddy rice for being obtained from above-mentioned F1 generation seed containing high yield multiresistance gene as female parent, from containing height
Paddy rice that the F1 ' of the anti-fall Drought-tolerant gene of high-quality obtains for seed is produced as male parent, human configuration cross combination obtains F0 " generation kind
Son, the F0 that will be obtained " obtains F1 for seed greenhouse adding generation is mixed " for seed, by molecular labeling assisting sifting go out containing high yield,
Multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 " is for seed；
(4) high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 are contained by above-mentioned " use greenhouse cultivation certainly for seed
Hand over, then go out to contain high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene progeny seed by molecular marker assisted selection；
(5) work of repeat step (4), i.e., select in selfing, the generation of selfing 5~7 to select high yield, stabilization, resist
Property, high-quality, anti-fall, drought-enduring new rice variety or new lines.
Wherein, in above-mentioned high-efficiency rice breeding method, the condition of the greenhouse adding generation is：Temperature control is 25~30
DEG C, relative humidity 70~85%.
Wherein, in above-mentioned high-efficiency rice breeding method, more than 85%, mass of 1000 kernel exists the setting percentage of the F1 generation seed
More than 30g.
Wherein, in above-mentioned high-efficiency rice breeding method, the F1 ' for seed setting percentage more than 82%, mass of 1000 kernel exists
More than 28g.
Wherein, in above-mentioned high-efficiency rice breeding method, the F1 " for seed setting percentage more than 90%, mass of 1000 kernel exists
More than 31g.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention makes full use of the excellent of kind on parental apolegamy
Gesture is complementary, is transformed for the shortcoming that offspring is likely to occur, in transformation process, from advantages and other shapes are close
Material is as parent so as to accelerate offspring's stabilized speed.Also, mutually tied with greenhouse adding generation using molecular marker assisted selection
The advanced breeding technique for closing, effectively shortens breeding time, improves efficiency of selection.New lines have high yield, disease resistance, lodging resistance
The characteristics of height, setting percentage (setting percentage is more than 92%) high, drought resisting, suit large area to popularize plantation, can reach cost-saving synergistic,
Promote the purpose of increasing peasant income.
Specific embodiment
The invention provides a kind of high-efficiency rice breeding method, the method is comprised the following steps：
(1) Panjin two and an aromatic plant metioned in ancient books excellent 822 human configuration cross combination of high yield of the selection with high yield multiresistance, obtain F0 generations
Seed, F1 generation seed is obtained by the F0 of acquisition for seed greenhouse adding generation is mixed, goes out to contain high yield by molecular labeling assisting sifting
The F1 generation seed of multiresistance gene；More than 85%, mass of 1000 kernel is in more than 30g for the setting percentage of the F1 generation seed；
(2) the anti-fall imperial round-grained rice 46 of selection high yield and high quality and drought-enduring two excellent 2161 human configuration cross combinations, obtain F0 ' generations
Seed, the F0 ' for obtaining is gone out containing high for seed for the mixed F1 ' that obtains of seed greenhouse adding generation by molecular labeling assisting sifting
The F1 ' of the anti-fall Drought-tolerant gene of high-quality is produced for seed；The F1 ' for seed setting percentage more than 82%, mass of 1000 kernel 28g with
On；
(3) paddy rice for being obtained from above-mentioned F1 generation seed containing high yield multiresistance gene as female parent, from containing height
Paddy rice that the F1 ' of the anti-fall Drought-tolerant gene of high-quality obtains for seed is produced as male parent, human configuration cross combination obtains F0 " generation kind
Son, the F0 that will be obtained " obtains F1 for seed greenhouse adding generation is mixed " for seed, by molecular labeling assisting sifting go out containing high yield,
Multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 " is for seed；The F1 " for seed setting percentage more than 90%, mass of 1000 kernel exists
More than 31g；
(4) high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 are contained by above-mentioned " use greenhouse cultivation certainly for seed
Hand over, then go out to contain high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene progeny seed by molecule assisted Selection；
(5) work of repeat step (4), i.e., select in selfing, the generation of selfing 5~7 to select high yield, stabilization, resist
Property, high-quality, anti-fall, drought-enduring new rice variety or new lines.
Wherein, in above-mentioned high-efficiency rice breeding method, the condition of the greenhouse adding generation is：Temperature control is 25~30
DEG C, relative humidity 70~85%.
Method used in the present invention unless otherwise specified, is this area conventional method.
Test material used, reagent etc. in following embodiments, unless otherwise specified, commercially obtain.Institute
Each crop varieties (strain) for using are conventional use of kind (strain) in breeding field, by country authorization assert or
Technical appraisement, can obtain or market purchasing in species bank.
Embodiment 1
High-efficiency rice breeding method, the method is comprised the following steps：
(1) Panjin two and an aromatic plant metioned in ancient books excellent 822 human configuration cross combination of high yield of the selection with high yield multiresistance, obtain F0 generations
Seed, F1 generation seed is obtained by the F0 of acquisition for seed greenhouse adding generation is mixed, goes out to contain high yield by molecular labeling assisting sifting
The F1 generation seed of multiresistance gene；, 86%, mass of 1000 kernel is in 31g for the setting percentage of F1 generation seed；Greenhouse add for when breeding condition
For：At 25~30 DEG C, relative humidity is controlled 70~85% temperature control；
(2) the anti-fall imperial round-grained rice 46 of selection high yield and high quality and drought-enduring two excellent 2161 human configuration cross combinations, obtain F0 ' generations
Seed, the F0 ' for obtaining is gone out containing high for seed for the mixed F1 ' that obtains of seed greenhouse adding generation by molecular labeling assisting sifting
The F1 ' of the anti-fall Drought-tolerant gene of high-quality is produced for seed；F1 ' for seed setting percentage 84%, mass of 1000 kernel is in 29g；Greenhouse add for when
Breeding condition be：At 25~30 DEG C, relative humidity is controlled 70~85% temperature control；
(3) paddy rice for being obtained from above-mentioned F1 generation seed containing high yield multiresistance gene as female parent, from containing height
Paddy rice that the F1 ' of the anti-fall Drought-tolerant gene of high-quality obtains for seed is produced as male parent, human configuration cross combination obtains F0 " generation kind
Son, the F0 that will be obtained " obtains F1 for seed greenhouse adding generation is mixed " for seed, by molecular labeling assisting sifting go out containing high yield,
Multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 " is for seed；F1 " for seed setting percentage 92%, mass of 1000 kernel is in 33g；Greenhouse
Plus for when breeding condition be：At 25~30 DEG C, relative humidity is controlled 70~85% temperature control；
(4) high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 are contained by above-mentioned " use greenhouse cultivation certainly for seed
Hand over, then go out to contain high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene progeny seed by molecular marker assisted selection；Greenhouse
Plus for when breeding condition be：Temperature control is in 25~30 DEG C, relative humidity 70~85%；
(5) work of repeat step (4), i.e., select in selfing, the generation of selfing 6 to select high yield, stabilization, multiresistance,
High-quality, anti-fall, drought-enduring new rice variety or new lines.
Embodiment 2
High-efficiency rice breeding method, the method is comprised the following steps：
(1) Panjin two and an aromatic plant metioned in ancient books excellent 822 human configuration cross combination of high yield of the selection with high yield multiresistance, obtain F0 generations
Seed, F1 generation seed is obtained by the F0 of acquisition for seed greenhouse adding generation is mixed, goes out to contain high yield by molecular labeling assisting sifting
The F1 generation seed of multiresistance gene；, 87%, mass of 1000 kernel is in 31g for the setting percentage of F1 generation seed；Greenhouse add for when breeding condition
For：At 25~30 DEG C, relative humidity is controlled 70~85% temperature control；
(2) the anti-fall imperial round-grained rice 46 of selection high yield and high quality and drought-enduring two excellent 2161 human configuration cross combinations, obtain F0 ' generations
Seed, the F0 ' for obtaining is gone out containing high for seed for the mixed F1 ' that obtains of seed greenhouse adding generation by molecular labeling assisting sifting
The F1 ' of the anti-fall Drought-tolerant gene of high-quality is produced for seed；F1 ' for seed setting percentage 84%, mass of 1000 kernel is in 29g；Greenhouse add for when
Breeding condition be：At 25~30 DEG C, relative humidity is controlled 70~85% temperature control；
(3) paddy rice for being obtained from above-mentioned F1 generation seed containing high yield multiresistance gene as female parent, from containing height
Paddy rice that the F1 ' of the anti-fall Drought-tolerant gene of high-quality obtains for seed is produced as male parent, human configuration cross combination obtains F0 " generation kind
Son, the F0 that will be obtained " obtains F1 for seed greenhouse adding generation is mixed " for seed, by molecular labeling assisting sifting go out containing high yield,
Multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 " is for seed；F1 " for seed setting percentage 93%, mass of 1000 kernel is in 33g；Greenhouse
Plus for when breeding condition be：Temperature control is in 25~30 DEG C, relative humidity 70~85%；
(4) high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 are contained by above-mentioned " use greenhouse cultivation certainly for seed
Hand over, then go out to contain high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene progeny seed by molecular marker assisted selection；
(5) work of repeat step (4), i.e., select in selfing, the generation of selfing 6 to select high yield, stabilization, multiresistance,
High-quality, anti-fall, drought-enduring new rice variety or new lines.
Through planting experimentally, the rice new strain time of infertility that the present invention is cultivated is 142~150 days, 90~100cm of plant height,
Plant type is compact, and colony's regularity is good, and anti-fall, strong drought resistance, sword-like leave is endured, and growth is delicate and pretty, and tillering ability is He the better-than-average , Miho types of Cheng Miho rates
Greatly.Comprehensive disease resistance is stronger, anti-seedling pest high, and Zhong Kang Miho neck pest, anti-stripe virus disease high has fragmentary false smut.Per mu yield is up to 610
~660Kg, its yield forming is：Every mu 21~230,000 fringes, per total grain panicle number 140~145, setting percentage more than 92% is (most suitable to broadcast
Phase setting percentage is planted up to more than 96%), 32~34g of mass of 1000 kernel.Seed rice in plantation, it is necessary to pass through to cultivate the strong seedling of many tillers, rationally
Dense planting, promotees the early enough seedlings of early hair and puts field in time, improves Population Quality, accomplishes fluently high yield basis.On the basis of suitable spike number is ensured,
Promote the big fringe of strong bar, so as to reach fringe foot, the purpose that fringe is big, grain takes back again high yield.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (5)
1. high-efficiency rice breeding method, it is characterised in that comprise the following steps：
(1) in Panjin two and an aromatic plant metioned in ancient books excellent 822 human configuration cross combination of high yield of the selection with high yield multiresistance, acquisition F0 generations, plant
Son, F1 generation seed is obtained by the F0 of acquisition for seed greenhouse adding generation is mixed, is gone out by molecule assisting sifting and is contained high yield multiresistance
The F1 generation seed of gene；
(2) the anti-fall imperial round-grained rice 46 of selection high yield and high quality and drought-enduring two excellent 2161 human configuration cross combinations, obtain F0 ' generation kinds
Son, F1 ' is obtained for seed by the F0 ' for obtaining for seed greenhouse adding generation is mixed, goes out to contain high yield and high quality by molecule assisting sifting
The F1 ' of anti-fall Drought-tolerant gene is for seed；
(3) paddy rice that the above-mentioned F1 generation seed containing high yield multiresistance gene is obtained is selected as female parent, from excellent containing high yield
The paddy rice that the F1 ' of the anti-fall Drought-tolerant gene of matter is obtained for seed obtains F0 as male parent, human configuration cross combination " for seed, will
The F0 of acquisition " obtains F1 for seed greenhouse adding generation is mixed " for seed, gone out containing high yield, multiresistance, excellent by molecule assisting sifting
Matter, anti-fall, Drought-tolerant gene F1 " is for seed；
(4) high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene F1 are contained by above-mentioned " greenhouse cultivation selfing is used for seed, then
Gone out by molecule assisted Selection and contain high yield, multiresistance, high-quality, anti-fall, Drought-tolerant gene progeny seed；
(5) work of repeat step (4), i.e., select in selfing, and the generation of selfing 5~7 is to selecting high yield, stabilization, multiresistance, excellent
Matter, anti-fall, drought-enduring new rice variety or new lines.
2. high-efficiency rice breeding method according to claim 1, it is characterised in that the condition of the greenhouse adding generation
For：Temperature control is in 25~30 DEG C, relative humidity 70~85%.
3. high-efficiency rice breeding method according to claim 1, it is characterised in that the setting percentage of the F1 generation seed exists
More than 85%, mass of 1000 kernel is in more than 30g.
4. high-efficiency rice breeding method according to claim 1, it is characterised in that the F1 ' exists for the setting percentage of seed
More than 82%, mass of 1000 kernel is in more than 28g.
5. high-efficiency rice breeding method according to claim 1, it is characterised in that the F1 " exists for the setting percentage of seed
More than 90%, mass of 1000 kernel is in more than 31g.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122145A (en) * | 2018-09-30 | 2019-01-04 | 上海市农业生物基因中心 | A kind of saving water, resisting drought rice based on batch production breeding system adds for method |
| CN109997634A (en) * | 2019-04-02 | 2019-07-12 | 宁夏金灵州种业有限公司 | A kind of late-maturing paddy rice planting method |
| CN110050688A (en) * | 2019-04-09 | 2019-07-26 | 武汉弘耕种业有限公司 | A kind of selection of hybrid rice restorer line |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285780A (en) * | 2014-10-16 | 2015-01-21 | 江苏瑞华农业科技有限公司 | High-yield multi-resistant rice breeding method |
-
2017
- 2017-03-21 CN CN201710170128.6A patent/CN106718853A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285780A (en) * | 2014-10-16 | 2015-01-21 | 江苏瑞华农业科技有限公司 | High-yield multi-resistant rice breeding method |
Non-Patent Citations (2)
| Title |
|---|
| 国家水稻数据中心: "龙粳46", 《国家水稻数据中心》 * |
| 黄献光等: "我国育出水旱兼用高产稻", 《北京农业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122145A (en) * | 2018-09-30 | 2019-01-04 | 上海市农业生物基因中心 | A kind of saving water, resisting drought rice based on batch production breeding system adds for method |
| CN109997634A (en) * | 2019-04-02 | 2019-07-12 | 宁夏金灵州种业有限公司 | A kind of late-maturing paddy rice planting method |
| CN110050688A (en) * | 2019-04-09 | 2019-07-26 | 武汉弘耕种业有限公司 | A kind of selection of hybrid rice restorer line |
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