CN106701556A - Digital PCR (Polymerase Chain Reaction) detection device and liquid path system thereof - Google Patents
Digital PCR (Polymerase Chain Reaction) detection device and liquid path system thereof Download PDFInfo
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
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- G01N35/1065—Multiple transfer devices
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
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Abstract
The invention relates to a digital PCR (Polymerase Chain Reaction) detection device and a liquid path system thereof. The digital PCR detection device comprises a detection chip, a liquid conveying mechanism and a sample conveying mechanism, wherein a diluent channel and a sample channel are formed in the detection chip; the liquid conveying mechanism is used for conveying a diluent into the diluent channel; the sample conveying mechanism is used for conveying a sample into the sample channel; a filtering chip is connected between the sample conveying mechanism and the detection chip; a first filtering pipeline is communicated between the liquid inlet of the filtering chip and the sample conveying mechanism; a second filtering pipeline is communicated between the liquid outlet of the filtering chip and the sample channel. Before being fed into the sample channel of the detection chip by the sample conveying mechanism, the sample passes through the filtering chip firstly, and impurities of the sample can be filtered off by the filtering chip without damaging liquid drops, so that the detection channel of the detection chip can be prevented from being blocked, the replacement frequency of the detection chip can be reduced, frequent focusing can be avoided, the detection efficiency can be improved, and the experiment cost can be lowered; in addition, after impurities and bubbles of the sample are filtered off by the filtering chip, the false judgment probability of an optical system on the liquid drops can be also reduced, and the detection results can be relatively precise.
Description
Technical field
The present invention relates to digital pcr detection technique field, more particularly to a kind of digital pcr detection means and its fluid path system
System.
Background technology
The accuracy of detection of digital pcr (Polymerase chain reaction, polymerase chain reaction) detection means requirement
It is very high, it is desirable to be able to which that each is believed by the fluorescence included in the liquid droplet of detection channel in accurate detection micro-fluidic chip
Number size, therefore it is required that Systems for optical inspection hot spot precisely focusing to Air conduct measurement section.The channel size of micro-fluidic chip is micro-
Not, often there is situation about blocking in meter level, cause chip to need often to change, more because of the impurity of sample in use
Changing rear chip needs to focus again, and Systems for optical inspection is higher to test position required precision, and focus process and structure are very
Complexity, the single test cycle lengthens, and detection efficiency is low；And chip structure is expensive, often changes and cause experimental cost to improve.
The content of the invention
It is an object of the invention to provide a kind of digital pcr detection means and its liquid-way system, detection chip can be reduced
Replacement frequency, improves detection efficiency, reduces experimental cost.
To realize the purpose of the present invention, adopt the technical scheme that：
A kind of digital pcr liquid-way system, including be provided with the detection chip of dilution passage and sample channel, give dilution
The conveying mechanism for entering dilution passage and the sample feeding mechanism that sample is sent into sample channel, between sample feeding mechanism and detection chip
Filtrating chip is connected with, it is communicated with the first filter pipeline between the inlet and sample feeding mechanism of filtrating chip, filtrating chip goes out
The second filter pipeline is communicated between liquid mouthful and sample channel.
Before sample feeding mechanism is by the sample channel of sample feeding detection chip, sample first passes through filtrating chip, filtrating chip
Impurity to sample is filtered, and without damaging drop, prevents the detection channel of detection chip from blocking, and reduces detection chip more
Frequency is changed, without frequently focusing again, detection efficiency is improved, experimental cost is reduced；And filtrating chip filters out the impurity of sample
After bubble, Systems for optical inspection is also reduced to drop probability of miscarriage of justice, make testing result more accurate.
Technical scheme is further illustrated below：
It is further that digital pcr liquid-way system also includes first pressure sensor and second pressure sensor, the first pressure
Force snesor is located at on the first filter pipeline, and second pressure sensor is on the second filter pipeline.Before and after filtrating chip
Coordinate pressure sensor, the pressure of filtrating chip is detected by first pressure sensor and second pressure sensor, when both
When pressure difference value is more than setting value, then filtrating chip is changed, it is ensured that to the filter effect of sample impurity, and be easy to supervision
And control.
It is further that digital pcr liquid-way system also includes sending into cleaning fluid the wiper mechanism and and sample of filtrating chip
The retracting device of product channel connection, wiper mechanism is provided with the first detergent line connected with the first filter pipeline.Cleaning solution first leads to
The first cleaning channels into the first filter pipeline are crossed, then filtrating chip is flowed into from the inlet of filtrating chip, to filtrating chip
Positive cleaning is carried out, the second filter pipeline is flowed out into from the liquid outlet of filtrating chip again afterwards, subsequently into detection chip
Sample channel, to sample channel be also carried out cleaning, finally enter retracting device, at the same realize to filtrating chip and detection chip
Cleaning, further prevent filtrating chip and detection chip from blocking, it is ensured that the strainability of filtrating chip, make making for filtrating chip
It is longer with the life-span, reduce detection chip and filtrating chip replacement frequency；And retracting device is mixed to the sample and cleaning solution after detection
Compound is reclaimed, when testing result outlet is abnormal, duplicate detection reproduction experiments after the drop separation that will can be reclaimed.
It is further that wiper mechanism is additionally provided with the second cleaning channels for being connected with the second filter pipeline and located at second
The first kinetic pump on cleaning channels, retracting device is also connected with the first filter pipeline.First kinetic pump is cleaning solution feeding the
Two cleaning channels, cleaning solution enters the second filter pipeline from the second cleaning channels, is then flowed into from the liquid outlet of filtrating chip
Filter element slice, reverse cleaning is carried out to filtrating chip, flows out into the second filter pipeline from the inlet of filtrating chip again afterwards,
Retracting device is finally entered, individually filtrating chip is cleaned, be further ensured that the strainability of filtrating chip, make filtration core
The service life of piece is longer, reduces detection chip and filtrating chip replacement frequency.
It is further that digital pcr liquid-way system also includes the first triple valve, the first triple valve is provided with and the second screen pipe
The first interface second interface connected with the second cleaning channels and the 3rd interface connected with sample channel of road connection；First
In first state, first interface is connected triple valve with second interface, and first interface and the 3rd interface disconnect；First triple valve exists
During the second state, first interface and second interface disconnect, first interface and the 3rd orifice.Sample is detected
When, the second filter pipeline is connected with sample channel by the first triple valve, and the second cleaning channels and the second filter pipeline are disconnected
Open, sample after filtering is detected into detection chip；When needing to carry out separate cleaning to filtrating chip, then by first
Triple valve disconnects the second cleaning channels and the second filter pipeline, and cleaning solution sequentially passes through the second cleaning channels, the second screen pipe
Road enters filtrating chip, and reverse cleaning is carried out to filtrating chip, makes that the service life of filtrating chip is longer, and filtrating chip makes for a long time
With not blocking；And the fluid path of liquid-way system is arranged more succinct by the first triple valve.
It is further that sample feeding mechanism includes PCR pipe, the second triple valve, liquid storage pipeline and with liquid storage pipeline connection the
Two kinetic pumps, the second triple valve is provided with the 5th interface that is connected with PCR pipe with the 4th interface of liquid storage pipeline connection and with
6th interface of one filter pipeline connection；Second triple valve in first state, the 4th interface and the 5th orifice, the 4th connects
Mouth disconnects with the 6th interface；In the second state, the 4th interface and the 5th interface disconnect second triple valve, the 4th interface and the 6th
Orifice.When sample feeding mechanism is sampled, by the second triple valve by PCR pipe and liquid storage pipeline connection, liquid storage pipeline and
One filter pipeline is disconnected, and sample is extracted into liquid storage pipeline by the second kinetic pump from PCR pipe；When sample feeding mechanism carries out sample presentation, by
Two triple valves connect liquid storage pipeline and the first filter pipeline, and liquid storage pipeline and PCR pipe disconnect, and the second kinetic pump is by sample from storage
Liquid pipeline feeding filtrating chip filtering, is detected, and make liquid-way system by the second triple valve subsequently into detection chip
Fluid path arrangement is more succinct.
It is further that digital pcr liquid-way system also includes the first Clean oil bottle for carrying cleaning solution, sample feeding mechanism
Also include the 3rd triple valve, the 3rd triple valve is provided with the connected with the second kinetic pump with the 7th interface of liquid storage pipeline connection
Eight interfaces and the 9th interface connected with the first Clean oil bottle；In first state, the 7th interface connects 3rd triple valve with the 8th
Mouth connection, the 7th interface and the 9th interface disconnect；In the second state, the 7th interface and the 8th interface disconnect 3rd triple valve,
7th interface and the 9th orifice.Sample feeding mechanism is sampled during with sample presentation, by the 3rd triple valve by liquid storage pipeline and
Two kinetic pumps are connected, and liquid storage pipeline and the first Clean oil bottle disconnect；When needing to clean filtrating chip, by the 3rd threeway
Valve connects liquid storage pipeline and the first Clean oil bottle, and fluid reservoir road and the second kinetic pump disconnect, and cleaning solution passes sequentially through the three or three
Port valve, liquid storage pipeline, the second triple valve and the first filter pipeline enter filtrating chip, and positive cleaning is carried out to filtrating chip, make
The cleaning function of the liquid-way system is stronger, and the fluid path of liquid-way system is arranged more succinct by the 3rd triple valve.
It is further that conveying mechanism includes the 3rd kinetic pump, the 4th triple valve, the second cleaning for carrying cleaning solution
Oil bottle, is communicated with connecting line between the 8th interface and the second kinetic pump, be communicated between the second Clean oil bottle and connecting line
3rd cleaning channels, the 3rd cleaning channels are provided with check valve, and the 4th triple valve is provided with the tenth connected with the 3rd kinetic pump and connects
The 12nd interface that mouth is connected with the 11st interface of dilution channel connection and with the second Clean oil bottle；4th triple valve exists
During first state, the tenth interface and the 11st orifice, the tenth interface and the 12nd interface disconnect；4th triple valve is second
During state, the tenth interface disconnects with the 11st interface, the tenth interface and the 12nd orifice.Will by the 4th triple valve
3rd kinetic pump and dilution channel connection, kinetic pump and the second Clean oil bottle disconnect, and dilution is sent into and examined by the 3rd kinetic pump
Survey chip；The 3rd kinetic pump and dilution passage are disconnected by the 4th triple valve, kinetic pump is connected with the second Clean oil bottle, the
Three kinetic pumps extract cleaning fluid from the 3rd Clean oil bottle, and the 4th triple valve is then passed through again by the 3rd kinetic pump and dilution passage
Connection, then cleaning fluid is carried out into detection chip, individually cleaning is carried out to detection chip；And second kinetic pump can also be by the 3rd
Cleaning channels will extract cleaning solution from the second Clean oil bottle, then cleaning fluid is sequentially passed through into the 3rd triple valve, liquid storage pipe, the two or three
Port valve and the first filter pipeline, positive cleaning is carried out into filtrating chip, allows the liquid-way system by different fluid path pair
Filtrating chip is cleaned, and makes the cleaning function to filtrating chip of the liquid-way system stronger.
It is further that the side of the lateral liquid outlet for having inlet in filtrating chip has been sequentially communicated the first filtering
Chamber, the second filter chamber and the 3rd filter chamber, multiple filterings are distributed with the first filter chamber, the second filter chamber and the 3rd filter chamber
Part, wherein the filtration members distribution density in the second filter chamber is than the filtration members distribution density in the first filter chamber and the 3rd filter chamber
Greatly.It is easy to the impurity direction filtered during by positive sample introduction to wash out.
The present invention also provides a kind of digital pcr detection means, including Systems for optical inspection and above-mentioned digital pcr fluid path
System.Digital pcr detection means with the digital pcr liquid-way system, makes digital pcr detection means reduction detection chip more
Change frequency, it is to avoid Systems for optical inspection is frequently focused again, improve detection efficiency, reduce experimental cost.
Compared with prior art, the invention has the advantages that：
Before sample feeding mechanism of the invention is by the sample channel of sample feeding detection chip, sample first passes through filtrating chip,
Filtrating chip is filtered to the impurity of sample, without damaging drop, prevents the detection channel of detection chip from blocking, and reduces detection
The replacement frequency of chip, without frequently focusing again, improves detection efficiency, reduces experimental cost；And filtrating chip filters out sample
After the impurity and bubble of product, Systems for optical inspection is also reduced to drop probability of miscarriage of justice, make testing result more accurate.
Brief description of the drawings
Fig. 1 is the structural representation of embodiment of the present invention digital pcr liquid-way system；
Fig. 2 is the structural representation of embodiment of the present invention filtrating chip.
Description of reference numerals：
10. detection chip, 110. dilution passages, 120. sample channels, 20. sample feeding mechanisms, 210.PCR pipes, 220. the
Two triple valves, 221. the 4th interfaces, 222. the 5th interfaces, 223. the 6th interfaces, 230. liquid storage pipelines, 240. second kinetic pumps,
250. connecting lines, 260. the 3rd triple valves, 261. the 7th interfaces, 262. the 8th interfaces, 263. the 9th interfaces, 30. liquor charging machines
Structure, 310. the 3rd kinetic pumps, 320. the 4th triple valves, 321. the tenth interfaces, 322. the 11st interfaces, 323. the 12nd interfaces,
330. second Clean oil bottles, 340. the 3rd cleaning channels, 350. first check valves, 40. filtrating chips, 410. first screen pipes
Road, 420. second filter pipelines, 430. first filter chambers, 440. second filter chambers, 450. the 3rd filter chambers, 460. filtration members,
470. first pressure sensors, 480. second pressure sensors, 50. retracting devices, 510. collection vessels, 520. peristaltic pumps,
530. waste liquid bottles, 540. second check valves, 610. first Clean oil bottles, 620. second cleaning channels, 630. first kinetic pumps,
70. first triple valves, 710. first interfaces, 720. second interfaces, 730. the 3rd interfaces, 80. the 5th triple valves, 810. the 13rd
Interface, 820. the 14th interfaces, 830. the 15th interfaces, 90. the 6th triple valves.
Specific embodiment
Embodiments of the invention are described in detail below in conjunction with the accompanying drawings：
As shown in figure 1, a kind of digital pcr fluid path device, including Systems for optical inspection and digital pcr liquid-way system, the number
Word PCR liquid-way systems dilute including being provided with the detection chip 10 of dilution passage 110 and sample channel 120, sending into dilution
The conveying mechanism 30 of liquid passage 110 and the sample feeding mechanism 20 by sample feeding sample channel 120, sample feeding mechanism 20 and detection core
Filtrating chip 40 is connected between piece 10, the first filter pipeline is communicated between the inlet and sample feeding mechanism 20 of filtrating chip 40
410, it is communicated with the second filter pipeline 420 between the liquid outlet and sample channel 120 of filtrating chip 40.
Before sample feeding mechanism 20 is by the sample channel 120 of sample feeding detection chip 10, sample first passes through filtrating chip 40,
Filtrating chip 40 is filtered to the impurity of sample, without damaging drop, prevents the detection channel of detection chip 10 from blocking, and is reduced
The replacement frequency of detection chip 10, without frequently focusing again, improves detection efficiency, reduces experimental cost；And filtrating chip 40
After filtering out the impurity and bubble of sample, Systems for optical inspection is also reduced to drop probability of miscarriage of justice, make testing result more accurate.
In the present embodiment, sample feeding mechanism 20 include PCR pipe 210, the second triple valve 220, liquid storage pipeline 230 and with storage
Liquid pipeline 230 connection the second kinetic pump 240, the second triple valve 220 be provided with connected with liquid storage pipeline 230 the 4th interface 221,
The 5th interface 222 connected with PCR pipe 210 and the 6th interface 223 connected with the first filter pipeline 410；Second triple valve
220 in first state, and the 4th interface 221 is connected with the 5th interface 222, and the 4th interface 221 disconnects with the 6th interface 223；The
In the second state, the 4th interface 221 disconnects two triple valves 220 with the 5th interface 222, the 4th interface 221 and the 6th interface 223
Connection.When sample feeding mechanism 20 is sampled, PCR pipe 210 is connected with liquid storage pipeline 230 by the second triple valve 220, liquid storage pipe
The filter pipeline 410 of road 230 and first is disconnected, and sample is extracted into liquid storage pipeline 230 by the second kinetic pump 240 from PCR pipe 210；Sample presentation
When mechanism 20 carries out sample presentation, liquid storage pipeline 230 is connected with the first filter pipeline 410 by the second triple valve 220, liquid storage pipeline
230 and PCR pipe 210 disconnect, sample is sent into filtrating chip 40 and filtered by the second kinetic pump 240 from liquid storage pipeline 230, subsequently into
Detection chip 10 is detected, and the fluid path of liquid-way system is arranged more succinct by the second triple valve 220.Sample feeding mechanism 20 is also
Other structure types that sample is sent into sample channel 120 can be according to actual needs set to, filtrating chip 40 can be with root
Set in PCR pipe 210 according to being actually needed.
As shown in figure 1, conveying mechanism 30 is including the 3rd kinetic pump 310, the 4th triple valve 320, for carrying cleaning solution
Second Clean oil bottle 330, connecting line 250, the second Clean oil bottle are communicated between the kinetic pump 240 of liquid storage pipeline 230 and second
The 3rd cleaning channels 340 are communicated between 330 and connecting line 250, the 3rd cleaning channels 340 are provided with the first check valve 350,
Cleaning fluid can only flow to connecting line 250 from the second Clean oil bottle 330, and the 4th triple valve 320 is provided with and connects with the 3rd kinetic pump 310
The 11st interface 322 that the tenth logical interface 321 is connected with dilution passage 110 and connected with the second Clean oil bottle 330
12nd interface 323；In first state, the tenth interface 321 is connected 4th triple valve 320 with the 11st interface 322, and the tenth connects
Mouth 321 disconnects with the 12nd interface 323；In the second state, the tenth interface 321 connects 4th triple valve 320 with the described 11st
Mouth 322 is disconnected, and the tenth interface 321 is connected with the 12nd interface 323.By the 4th triple valve 320 by the 3rd kinetic pump 310 and dilute
Release liquid passage 110 to connect, kinetic pump is disconnected with the second Clean oil bottle 330, and dilution is sent into detection chip by the 3rd kinetic pump 310
10；The 3rd kinetic pump 310 and dilution passage 110 are disconnected by the 4th triple valve 320, kinetic pump and the second Clean oil bottle
330 connections, the 3rd kinetic pump 310 is extracted cleaning fluid, is then moved the 3rd by the 4th triple valve 320 again from the 3rd Clean oil bottle
Power pump 310 is connected with dilution passage 110, then cleaning fluid is carried out into detection chip 10, and individually cleaning is carried out to detection chip 10；
And second kinetic pump 240 can also will extract cleaning solution from the second Clean oil bottle 330 by the 3rd cleaning channels 340, then will be clear
Washing lotion sequentially passes through liquid storage pipe, the second triple valve 220 and the first filter pipeline 410, and positive cleaning is carried out into filtrating chip 40,
Make the service life of filtrating chip 40 longer, reduce detection chip 10 and the replacement frequency of filtrating chip 40.
As shown in figure 1, digital pcr liquid-way system also include by cleaning fluid feeding filtrating chip 40 wiper mechanism and with
The retracting device 50 of the connection of sample channel 120, wiper mechanism includes the first Clean oil bottle 610 for carrying cleaning solution, detection
The dilution passage 110 of chip 10 is connected with sample channel 120, and sample feeding mechanism 20 also includes the 3rd triple valve 260, the 3rd threeway
Valve 260 be provided with the 8th interface 262 that the 7th interface 261 connected with liquid storage pipeline 230 connects with the second kinetic pump 240 and with
9th interface 263 of the connection of the first Clean oil bottle 610；3rd triple valve 260 in first state, the 7th interface 261 and the 8th
Interface 262 is connected, and the 7th interface 261 disconnects with the 9th interface 263；3rd triple valve 260 in the second state, the 7th interface
261 are disconnected with the 8th interface 262, and the 7th interface 261 is connected with the 9th interface 263.Sample feeding mechanism 20 is sampled during with sample presentation,
Liquid storage pipeline 230 is connected with the second kinetic pump 240 by the 3rd triple valve 260, the Clean oil bottle of liquid storage pipeline 230 and first
610 disconnect；When needing to clean filtrating chip 40, by the 3rd triple valve 260 by the cleansing oil of liquid storage pipeline 230 and first
Bottle 610 is connected, and fluid reservoir road and the second kinetic pump 240 disconnect, and the cleaning solution in the first Clean oil bottle 610 passes through the 3rd kinetic pump
310 dynamic action, passes sequentially through the 3rd triple valve 260, liquid storage pipeline 230, the second triple valve 220 and the first filter pipeline
410 enter filtrating chip 40, and positive cleaning is carried out to filtrating chip 40, and detection chip 10 is cleaned again then, most laggard
Enter retracting device 50, while realizing the cleaning to filtrating chip 40 and detection chip 10, make the cleaning function of the liquid-way system more
By force, further prevent filtrating chip 40 and detection chip 10 from blocking, it is ensured that the strainability of filtrating chip 40, reduce detection chip
10 replacement frequency；And the sample and cleaning liquid mixture after 50 pairs of detections of retracting device are reclaimed, when testing result outlet is different
Chang Shi, duplicate detection reproduction experiments after the drop separation that will can be reclaimed, and the fluid path of liquid-way system is made by the 3rd triple valve 260
Arrangement is more succinct.
Wiper mechanism also includes the second cleaning channels 620 and the first kinetic pump 630 on the second cleaning channels 620,
Retracting device 50 is also connected with the first filter pipeline 410, and digital pcr liquid-way system also includes the first triple valve 70, the first threeway
Valve 70 is provided with the second interface that the first interface 710 connected with the second filter pipeline 420 is connected with the second cleaning channels 620
720 and the 3rd interface 730 that is connected with sample channel 120；First triple valve 70 in first state, first interface 710 and
Two interfaces 720 are connected, and first interface 710 disconnects with the 3rd interface 730；First triple valve 70 in the second state, first interface
710 are disconnected with second interface 720, and first interface 710 is connected with the 3rd interface 730.When being detected to sample, by
One triple valve 70 connects the second filter pipeline 420 with sample channel 120, and the second cleaning channels 620 and the second filter pipeline
420 are disconnected, and sample after filtering is detected into detection chip 10；When needing to carry out separate cleaning to filtrating chip 40,
Second cleaning channels 620 are disconnected with the second filter pipeline 420 by the first triple valve 70 then, the first kinetic pump 630 is cleaning
Liquid sends into the second cleaning channels 620, and cleaning solution enters the second filter pipeline 420 from the second cleaning channels 620, then from filtration core
The liquid outlet of piece 40 flows into filtrating chip 40, reverse cleaning is carried out to filtrating chip 40, afterwards again from the feed liquor of filtrating chip 40
Mouth flows out into the second filter pipeline 420, finally enters retracting device 50, individually carries out reverse cleaning to filtrating chip 40,
It is further ensured that the strainability of filtrating chip 40；And the fluid path of liquid-way system is arranged more succinct by the first triple valve 70.
As shown in figure 1, digital pcr liquid-way system also includes the 5th triple valve 80, the 5th triple valve 80 is provided with and the first mistake
The 14th interface 820 and and retracting device that 13rd interface 810 of the connection of chimney filter road 410 is connected with the second triple valve 220
15th interface 830 of 50 connections；In first state, the 13rd interface 810 connects 5th triple valve 80 with the 14th interface 820
Logical, i.e., the second filter pipeline 420 is connected with the second triple valve 220；5th triple valve 80 in the second state, the 13rd interface
810 are connected with the 15th interface 830, and the second filter pipeline of machine 420 is connected with retracting device 50, arrange the fluid path of liquid-way system
It is more succinct.
In the present embodiment, retracting device 50 includes that collection vessel 510, peristaltic pump 520, waste liquid bottle 530 and second are unidirectional
Valve 540, after the second check valve 540 is connected in parallel with peristaltic pump 520, then connects with waste liquid bottle 530, the 5th triple valve 80 and waste liquid
Bottle 530 is connected, and retracting device 50 is also provided with the 6th triple valve 90 between detection chip 10, and inspection is made by the 6th triple valve 90
Chip 10 is surveyed to be connected with collection vessel 510 or waste liquid bottle 530.Retracting device 50 can also according to actual needs use other shapes
Formula.
In sum, in the present embodiment, the liquid-way system is provided with three cleaning fluid path and filtrating chip 40 is carried out clearly
Wash, one of cleaning fluid path is that the cleaning solution in first Clean oil bottle 610 is passed sequentially through into the 3rd by the 3rd kinetic pump 310
Triple valve 260, liquid storage pipeline 230, the second triple valve 220, the 5th triple valve 80 and the first filter pipeline 410, to filtrating chip
40 carry out positive cleaning, and detection chip 10 is cleaned again afterwards；Another cleaning fluid path is will by the second kinetic pump 240
Cleaning solution in second Clean oil bottle 330 passes sequentially through the 3rd triple valve 260, liquid storage pipeline 230, the second triple valve the 220, the 5th
The filter pipeline 410 of triple valve 80 and first, positive cleaning is carried out to filtrating chip 40, and detection chip 10 is carried out clearly again afterwards
Wash；3rd cleaning fluid path is that cleaning fluid is passed sequentially through into the second cleaning channels 620, the first threeway by the first kinetic pump 630
The filter pipeline 420 of valve 70 and second, individually carries out reverse cleaning to filtrating chip 40.Make liquid-way system that there is powerful cleaning work(
Can, filtrating chip 40 can be cleaned by different cleaning fluid path according to actual needs, using more flexible.
In the present embodiment, as shown in Fig. 2 the side for having a lateral liquid outlet of inlet in filtrating chip 40 connects successively
It is connected with the first filter chamber 430, the second filter chamber 440 and the 3rd filter chamber 450, the first filter chamber 430, the and of the second filter chamber 440
Multiple filtration members 460 are distributed with 3rd filter chamber 450, wherein the distribution density ratio of filtration members 460 in the second filter chamber 440
The distribution density of filtration members 460 in first filter chamber 430 and the 3rd filter chamber 450 is big, and be easy to be filtered during by positive sample introduction is miscellaneous
Matter direction washes out.The filtration members 460 are Filter column, and the shape of filtration members 460 and distribution can also be carried out according to actual needs
Adjustment, filtrating chip 40 can also according to actual needs be set to other structures, and the structure of filtration members 460 of filtrating chip 40 can be same
When be applied at the feeder connection of detection chip 10.
As shown in figure 1, filter pipeline is provided with first pressure sensor 470 and second pressure for signal piping pressure
Sensor 480, first pressure sensor 470 is located at on the first filter pipeline 410, and second pressure sensor 480 is located at second
On filter pipeline 420.Coordinate pressure sensor before and after filtrating chip 40, by first pressure sensor 470 and second pressure
Sensor 480 detects the pressure of filtrating chip 40, when both pressure difference values are more than setting value, then filtrating chip 40 is carried out
Change, it is ensured that to the filter effect of sample impurity, and be easy to supervise and control.
Digital pcr fluid path device also includes being electrically connected with first pressure sensor 470 and second pressure sensor 480
Controller (accompanying drawing is not identified) and the alarm being electrically connected with controller (accompanying drawing is not identified).When first pressure sensor 470
And the pressure difference value between second pressure sensor 480 be more than setting value when, controller drive alarm equipment alarm, be more convenient for supervision
And control.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Can not therefore be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of digital pcr liquid-way system, it is characterised in that including be provided with dilution passage and sample channel detection chip,
Dilution is sent into the conveying mechanism of the dilution passage and sample is sent into the sample feeding mechanism of the sample channel, it is described
Be connected with filtrating chip between sample feeding mechanism and the detection chip, the inlet of the filtrating chip and the sample feeding mechanism it
Between be communicated with the first filter pipeline, the second screen pipe is communicated between the liquid outlet of the filtrating chip and the sample channel
Road.
2. digital pcr liquid-way system according to claim 1, it is characterised in that also including first pressure sensor and
Two pressure sensors, the first pressure sensor is located at on first filter pipeline, and the second pressure sensor sets
In on second filter pipeline.
3. digital pcr liquid-way system according to claim 1, it is characterised in that also including cleaning fluid is sent into the mistake
The wiper mechanism of filter element slice and the retracting device connected with the sample channel, the wiper mechanism are provided with and first mistake
Filter the first detergent line of pipeline connection.
4. digital pcr liquid-way system according to claim 3, it is characterised in that the wiper mechanism be additionally provided with it is described
Second cleaning channels and the first kinetic pump on second cleaning channels of the second filter pipeline connection, the recovery
Device is also connected with first filter pipeline.
5. digital pcr liquid-way system according to claim 4, it is characterised in that also including the first triple valve, described first
Triple valve be provided with the second interface that the first interface connected with second filter pipeline connects with second cleaning channels,
And the 3rd interface connected with the sample channel；First triple valve in first state, the first interface with it is described
Second interface is connected, and the first interface disconnects with the 3rd interface；First triple valve in the second state, described
One interface disconnects with the second interface, the first interface and the 3rd orifice.
6. the digital pcr liquid-way system according to claim 1 or 3, it is characterised in that the sample feeding mechanism include PCR pipe,
Second triple valve, liquid storage pipeline and the second kinetic pump with the liquid storage pipeline connection, second triple valve be provided with it is described
The 5th interface that 4th interface of liquid storage pipeline connection is connected with the PCR pipe and connected with first filter pipeline
Six interfaces；Second triple valve in first state, the 4th interface and the 5th orifice, the 4th interface
Disconnected with the 6th interface；In the second state, the 4th interface disconnects second triple valve with the 5th interface,
4th interface and the 6th orifice.
7. described digital pcr liquid-way system according to claim 6, it is characterised in that also including cleaning for carrying
First Clean oil bottle of liquid, the sample feeding mechanism also includes the 3rd triple valve, and the 3rd triple valve is provided with and the liquid storage pipe
7th interface the 8th interface connected with second kinetic pump of road connection and connected with first Clean oil bottle the
Nine interfaces；3rd triple valve in first state, the 7th interface and the 8th orifice, the 7th interface
Disconnected with the 9th interface；In the second state, the 7th interface disconnects 3rd triple valve with the 8th interface,
7th interface and the 9th orifice.
8. digital pcr liquid-way system according to claim 7, it is characterised in that the conveying mechanism includes the 3rd power
Pump, the 4th triple valve, the second Clean oil bottle for carrying cleaning solution, connect between the 8th interface and second kinetic pump
Connecting line is connected with, the 3rd cleaning channels are communicated between second Clean oil bottle and the connecting line, the described 3rd is clear
Clean pipeline is provided with check valve, and the 4th triple valve is provided with the tenth interface that is connected with the 3rd kinetic pump and described dilute
The 12nd interface released the 11st interface of liquid channel connection and connected with second Clean oil bottle；4th triple valve
In first state, the tenth interface and the 11st orifice, the tenth interface are disconnected with the 12nd interface
Open；In the second state, the tenth interface disconnects 4th triple valve with the 11st interface, the tenth interface with
12nd orifice.
9. the digital pcr liquid-way system according to any one of claim 1 to 8, it is characterised in that have in the filtrating chip
The side of one lateral liquid outlet of inlet has been sequentially communicated the first filter chamber, the second filter chamber and the 3rd filter chamber, described
Multiple filtration members are distributed with one filter chamber, second filter chamber and the 3rd filter chamber, wherein second filtering
Filtration members distribution density in chamber is bigger than the filtration members distribution density in first filter chamber and the 3rd filter chamber.
10. a kind of digital pcr detection means, it is characterised in that including Systems for optical inspection and any one of claim 1 to 9 institute
The digital pcr liquid-way system stated.
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