CN106688887B - The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder - Google Patents
The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder Download PDFInfo
- Publication number
- CN106688887B CN106688887B CN201611123823.9A CN201611123823A CN106688887B CN 106688887 B CN106688887 B CN 106688887B CN 201611123823 A CN201611123823 A CN 201611123823A CN 106688887 B CN106688887 B CN 106688887B
- Authority
- CN
- China
- Prior art keywords
- bud
- culture
- differentiation
- fruit
- miscellaneous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the tissue culture and rapid propagation methods of miscellaneous No. 1 dwarf banana of powder, belong to field of plant tissue culture.The method of the present invention include the following steps: to select false stem it is green, it is sturdy, be highly 3.2~4.5m, fruit ear is larger, and fruit refers to that disjunctor fruit is less, and fruit shape is normal, and the good plant of quality is maternal plant；To the admixing medical solutions for inhaling bud spray carbendazim and streptomysin on maternal plant, it is that explant is inoculated with that suction bud is taken after 1 week；Break up cultivation temperature control at 27 DEG C~33 DEG C, differentiation 12~20 days progress subcultures of culture, subculture sum controlled in 12~16 generations；Culture of rootage and hardening.Miscellaneous No. 1 rooted seedling of powder bred using technology of the invention, root system is more, it is more sturdy and white, seedling vacation stem chartreuse, dark green leaf, it is planted high survival rate, growth is very fast and neat, and about 5000-8000 plants of seedling can be bred in each explant one-year age, and kind property is stablized after field planting, seldom there is variant, banana peasant's reflection is good.
Description
Technical field
The invention belongs to field of plant tissue culture, and in particular to the tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder.
Background technique
' miscellaneous No. 1 of powder ' dwarf banana, is the dwarf banana kind of the anti-blight of new breeding, by Guangdong Academy of Agricultural Sciences's fruit tree research
Institute, the research of bureau of agriculture, Zhongshan city are produced, and are the accidental seedling of No. 1 dwarf banana of wide powder.
For at present, although banana group culturation rapid propagating technology is commonly used, miscellaneous No. 1 dwarf banana of powder is four due to its genotype
Times body note any of several broadleaf plants ABBB type goes culture powder miscellaneous No. 1, breeding with common triploid banana (AAA type) tissue culture and rapid propagation method
Speed is slow, low efficiency, and rooted seedling transplanting survival is low, and in general obtainable proliferation times are only 1.3 times, one suction bud 1 year
Several hundred young plants are just bred, and seedling temporary planting survival rate is sometimes less than 20%.How ' powder miscellaneous No. 1 ' growth coefficient and hereditary is improved
Stabilization does not make a variation, great realistic meaning.
Summary of the invention
The purpose of the present invention is to provide the tissue culture and rapid propagation methods of miscellaneous No. 1 dwarf banana of powder.
The technical solution used in the present invention is:
The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder, including the following steps:
1) screen maternal plant: select false stem it is green, it is sturdy, highly be 3.2~4.5m, fruit ear is larger, and fruit refers to that disjunctor fruit is less,
Fruit shape is normal, and the good plant of quality is maternal plant；
2) explant inhales the processing of bud: to the admixing medical solutions for inhaling bud spray carbendazim and streptomysin, it is outer that suction bud is taken after 1 week
Implant is inoculated with；
3) differentiation culture: differentiation cultivation temperature control is at 27 DEG C~33 DEG C, differentiation 12~20 days progress subcultures of culture, subculture
Sum control is in 12~16 generations；
4) culture of rootage and hardening；Bud Differentiation is inoculated with root media, first week indoors under dim light or no light condition,
The long seedling of promoting root growth；Second week utilizes greenhouse hardening, and at 26-30 DEG C, natural light shade irradiation, intensity of illumination control exists for temperature control
4000-5000lux；After two weeks, intensity of illumination is adjusted to 8000-10000lux, until emergence.
Preferably, in step 1), planting any of several broadleaf plants maternal plant height is 3.2~3.5m, and perennial root any of several broadleaf plants maternal plant height is 3.8~4.5m.
If it exceeds lower than this height, it is possible to occur and the deterioration phenomenon that is not inconsistent of kind property.
The requirement of maternal plant is standardized in step 1), is to select and meet kind of a property, plant best in quality.If selected
False stem yellow, thin and weak, fruit ear is smaller, and fruit refers to that disjunctor fruit is more, and fruit shape is abnormal, and the bad plant of quality is maternal plant, it is possible to
There is the deterioration phenomenon not being inconsistent with kind property.
Preferably, in step 2, when inhale bud it is long to 30~50cm high when, to inhale bud spray 50% carbendazim, 1000 times of liquid and
The admixing medical solutions of the streptomysin of 150ppm concentration, it is that explant is inoculated with that suction bud is taken after 1 week.
When specifically used, in 1000 kilograms of admixing medical solutions contain 1 kilogram of 50% carbendazim, 150 grams of agricultural streptomycin, often
0.5 kilogram of bud irrigation liquid.
The admixing medical solutions of carbendazim and streptomysin are mainly to kill the endogenetic bacteria for inhaling bud inner meristem.
Inventor finally preferably goes out above two medicine and matched proportion density by screening, and obtained admixing medical solutions imitate bacterium
Fruit is best, and relative low price.
Preferably, in step 3), differentiation differential medium used when cultivating is to add to fit on the basis of MS culture medium
The basic element of cell division 6-BA, TDZ and KT-30 and auxin IBA and NAA are measured, control proliferation times are 2.5~2.8 times.
Specifically, the used culture medium of differentiation culture be added on the basis of MS culture medium 3~5mg/L obtain 6-BA,
The KT-30 of the TDZ of 0.004~0.015mg/L, 0.3~0.6mg/L, and appropriate IBA and NAA, adjust within the above range
The concentration of hormone, the size and height of control control differentiation sprout, so that proliferation rate control is at 2.5~2.8 times.
Proliferation times control is best in the 2.5-2.8 times of lower Bud Differentiation obtained.If the multiple that rises in value is too low, efficiency is influenced
And cost；If proliferation times are too high, bud is too many, and sprout is small, and the loose alveolation of Bud Differentiation leaf sheath, sprout is of poor quality, actually
Influence the reproduction speed of the seedling of whole process, it is also possible to develop into lopsided bud.
Preferably, in step 3), differentiation cultivation temperature control is at 29 DEG C~33 DEG C.
Break up cultivation temperature in the tissue-culturing rapid propagation of banana and is generally 25~28 DEG C, the miscellaneous No. 1 dwarf banana tissue culture of preferred powder of the present invention
Fast numerous middle differentiation cultivation temperature is 29 DEG C~33 DEG C, higher than the cultivation temperature of previous banana.Cultivation temperature is broken up when being lower than 29 DEG C
Bud is easy to grow up, and proliferation rate is lower；For cultivation temperature at 33 DEG C or more, the growth of Bud Differentiation early period is very fast, late-stage differentiation bud
Leaf sheath is loose, and sprout is of poor quality.It is cultivated at 29 DEG C~33 DEG C, Bud Differentiation is tender white sturdy, and sprout is more solid, highly suitable with thickness
In.
Preferably, in step 3), differentiation 15~18 days progress subcultures of culture, subculture sum was controlled in 13~15 generations.
Generally 20~30 days progress subcultures are cultivated in differentiation in the tissue-culturing rapid propagation of banana, and break up cultivated days control in the present invention
System in 15~18 days progresss subcultures, transfer by the subculture that do not exceed the time limit, to prevent Bud Differentiation aging, causes that big bud opens leaf and root no longer divides out
Change proliferation；Within 13~15 generation of algebra.
Preferably, in step 3) when subculture switching Bud Differentiation, Bud Differentiation part top first sheathing leaf is clipped in cutting, and longitudinal sectional big bud is small
Bulb, without separating bottom set, removes apical growth advantage, promotes lateral bud redifferentiation clump with leaf sheath intersection growing point separate living tissue
It is raw.
Because the low algebra Bud Differentiation of dwarf banana is coarse, it is strong to grow up gesture, simple bud easy to form, Kai Ye and root no longer divides out
Change.When therefore using subculture switching Bud Differentiation, the part top first sheathing leaf of Bud Differentiation is clipped in cutting, removes apical growth advantage.Together
When, longitudinal sectional big bud bottom set, without separating bottom set, promotes lateral bud redifferentiation to grow thickly with leaf sheath intersection growing point separate living tissue.
Preferably, in step 4) culture medium used in culture of rootage be on the basis of MS culture medium add 30g/L sucrose,
The NAA of the IBA and 0.5~0.8mg/L of 0.1~0.2mg/L.This formula help to obtain more and small root systems, improve temporary planting at
Motility rate.
The content of sucrose is definite value in the medium, and concentration height can be difficult to take root when taking root, because osmotic pressure is too big.
Preferably, in step 4), Bud Differentiation is inoculated with root media, first week indoors under dim light or no light condition,
The long seedling of promoting root growth, temperature are controlled at 25 DEG C -28 DEG C；Second week utilizes greenhouse hardening, and at 26 DEG C -30 DEG C, natural light is hidden for temperature control
Shade irradiation, intensity of illumination are controlled in 4000-5000lux；After 2 weeks, intensity of illumination is adjusted to 8000-10000lux.After i.e. in hardening
Phase light is eager to excel than banana seedlings.
The beneficial effects of the present invention are:
Tissue culture and rapid propagation method of the present invention is specific to this tetraploid banana variety of ' miscellaneous No. 1 of powder ' dwarf banana, is different from existing
There is the tissue culture and rapid propagation method of triploid banana common in technology.Using method of the invention, ' miscellaneous No. 1 of powder ' powder can solve
Any of several broadleaf plants reproduction speed is slow, low efficiency, and rooted seedling transplanting survival is low and Genomic instability, is easy the problem of variation etc..
Miscellaneous No. 1 rooted seedling of powder bred using technology of the invention, root system is more, more sturdy and white, differentiation and proliferation times
Number is 2.5-2.8 times, seedling vacation stem chartreuse, dark green leaf, and temporary planting survival rate may be up to 95% or more, growth comparatively fast and
Neatly, about 5000-8000 plants of seedling can be bred in each explant one-year age, kind property is stablized after field planting, seldom makes a variation
Strain, banana peasant's reflection are good.
The present invention standardizes the range of choice of maternal plant, in order to select and meet kind of a property, plant best in quality.Benefit
Tissue-culturing rapid propagation is carried out with the maternal plant of standard of the present invention, just can guarantee excellent kind.
The present invention uses the admixing medical solutions of carbendazim and streptomysin, can kill the endogenetic bacteria for inhaling bud inner meristem,
Effect is good, while relative low price.
Differential medium of the invention is that the appropriate basic element of cell division 6-BA, TDZ and KT- are added on the basis of MS culture medium
30 and auxin IBA and NAA, control proliferation times are 2.5~2.8 times.Proliferation times control obtains under 2.5~2.8 times
Bud Differentiation it is best.If the multiple that rises in value is too low, efficiency and cost are influenced；If proliferation times are too high, bud is too many, sprout
Small, the loose alveolation of Bud Differentiation leaf sheath, sprout is of poor quality, actually also influences the reproduction speed of the seedling of whole process, it is also possible to
Develop into lopsided bud.
Breaking up cultivation temperature in the miscellaneous No. 1 dwarf banana tissue-culturing rapid propagation of preferred powder of the present invention is 29 DEG C~33 DEG C, higher than common perfume (or spice)
The temperature of any of several broadleaf plants tissue-culturing rapid propagation differentiation culture.When cultivation temperature is lower than 29 DEG C, Bud Differentiation is easy to grow up, and proliferation rate is lower, training
Temperature is supported at 33 DEG C or more, the growth of Bud Differentiation early period is very fast, and late-stage differentiation bud-leaf sheath is loose, and sprout is of poor quality.29 DEG C~
It is cultivated at 33 DEG C, Bud Differentiation is tender white sturdy, and sprout is more solid, highly moderate with thickness.
The present invention breaks up cultivated days control in 15~18 days progress subcultures, subculture switching of not exceeding the time limit, to prevent Bud Differentiation
Aging causes big bud to open leaf and out root no longer differentiation and proliferation；Within 13~15 generation of algebra.
The invention also discloses subculture switching cutting technologies, remove apical growth advantage, lateral bud redifferentiation is promoted to grow thickly.
Root media of the invention suitably reduces IBA concentration, increases NAA concentration, and it is more and smaller to be conducive to root system,
Improve temporary planting survival rate.
Hardening culture middle and later periods light of the present invention is eager to excel than banana seedlings, and it is yellowish green for may advantageously facilitate the false Stem nematode of seedling
Color, leaf green, vitality are strong.
Detailed description of the invention
Fig. 1 is the miscellaneous No. 1 tissue culture Multiplying culture of powder；
Fig. 2 is that miscellaneous No. 1 greenhouse of powder is planted cup seedling.
Specific embodiment
Chinese and English initialism:
6-BA is 6- benzyl gland purine, and TDZ is N- benzyl phenyl-N'-1, and 2,3- thiadiazoles -5- ureas, KT-30 is the chloro- 4- of N-(2-
Pyridyl group)-N- phenylurea, IBA is indolebutyric acid, and NAA is niacin.
The present invention is further illustrated combined with specific embodiments below, and however, it is not limited to this.
Embodiment 1
Enforcement place: Fruit Tree Inst., Guangdong prov. Academy of Agricultural Sciences's maternal plant garden, time: November in October, 2012-.
(1) screening of maternal plant: select false stem it is green, it is sturdy, be highly 3.2~3.5m, fruit ear is larger, fruit refer to disjunctor fruit compared with
Few, fruit shape is normal, the good planting any of several broadleaf plants plant of quality, plants in greenhouse or isolation implant garden, as maternal plant garden, for taking bud to be used.
(2) explant inhales the processing of bud: when inhale bud it is long to 30-50cm when, to inhale bud spray 50% carbendazim, 1000 times of liquid and
The admixing medical solutions of 150ppm agricultural streptomycin.Specifically, contain 1 kilogram of 50% carbendazim in 1000 kilograms of admixing medical solutions, it is agricultural
150 grams of streptomysin, every bud fills 0.5 kilogram of admixing medical solutions；It is that explant is inoculated with that suction bud is taken after 1 week.
(3) differentiation culture: differentiation culture is packing container using vial or polypropylene film bag, and temperature is controlled at 29 DEG C
~33 DEG C.
Dwarf banana tissue culture proliferation times and temperature have strong dependency.Bud Differentiation Multiplying culture temperature is controlled at 31 ± 2 DEG C.Training
Support temperature be lower than 29 DEG C when Bud Differentiation be easy to grow up, proliferation rate is lower, cultivation temperature at 33 DEG C or more, early period Bud Differentiation
Growth is very fast, and late-stage differentiation bud-leaf sheath is loose, and sprout is of poor quality.
Differentiation 15-18 days progress subcultures of culture.The low algebra Bud Differentiation of dwarf banana is coarse, and it is strong to grow up gesture, list easy to form
Bud, Kai Ye and root no longer breaks up out.When subculture switching Bud Differentiation, the part top first sheathing leaf of Bud Differentiation, longitudinal sectional big bud are clipped in cutting
Bottom set, without separating bottom set, to remove apical growth advantage, promotes lateral bud point with leaf sheath intersection growing point separate living tissue
Change is grown thickly.Subculture sum controlled in 13~15 generations.
Differentiation culture culture medium used, including following component: on the basis of conventional fragrant tooth any of several broadleaf plants (AAA type) culture medium
The appropriate basic element of cell division 6-BA, TDZ and KT-30 and auxin IBA and NAA are added, the concentration of above-mentioned hormone, control point are adjusted
Change the size and height of sprout, so that proliferation times control is at 2.5-2.8 times, obtained Bud Differentiation grown junction is substantially excellent.
(4) culture of rootage and hardening: it is dense that root media used suitably reduces IBA on the basis of banana root media
Degree increases NAA concentration, promotes the root system of growth more and small, improves temporary planting survival rate.Specifically, root media includes following
Component: MS+ sucrose 30g/L+IBA0.1mg/L+NAA0.6mg/L.Culture of rootage is packing container with polypropylene film bag, every bag
It is inoculated with 8 plants of more sturdy Bud Differentiations of the same size.
Bud Differentiation is inoculated with root media, first week indoors under dim light or no light condition, the long seedling of promoting root growth；Second week benefit
With greenhouse hardening, at 26-30 DEG C, natural light shade irradiation, intensity of illumination is controlled in 4000-5000lux for temperature control；Two weeks
Afterwards, intensity of illumination is adjusted to 8000-10000lux, the same second week of other conditions.After four weeks tissue-cultured seedling stem enrich leaf dark green (see
Fig. 1), transplanting success is higher, up to 95% or more.
Using this technology, our unit's ten thousand young plant of annual fast numerous miscellaneous No. 1 dwarf banana 40-80 of powder, deeply by banana peasant's favorable comment after plantation.
Embodiment 2
Enforcement place: Fruit Tree Inst., Guangdong prov. Academy of Agricultural Sciences's maternal plant garden, the time: in October, 2013 starts.
(1) screening of maternal plant: select false stem it is green, it is sturdy, highly for the planting any of several broadleaf plants of 3.4m and 4.1m perennial root any of several broadleaf plants, fruit ear
Larger, fruit refers to that disjunctor fruit is less, and fruit shape is normal, the good plant of quality, as stock tree, for taking bud to be used.
(2) explant inhales the processing of bud: when inhale bud it is long to 30-50cm when, add to bud spray 50% carbendazim, 1000 times of liquid are inhaled
The admixing medical solutions of 150ppm agricultural streptomycin, every bud fill 0.5 kilogram of admixing medical solutions；It is that explant is inoculated with that suction bud is taken after 1 week.
(3) differentiation culture: differentiation culture is packing container using polypropylene film bag, and temperature is controlled at 29 DEG C~33 DEG C.
Differentiation 15 days progresss subcultures of culture, subculture transfer Bud Differentiation when, cut off and clip the part top first sheathing leaf of Bud Differentiation, longitudinal sectional big bud is small
Bulb, without separating bottom set, to remove apical growth advantage, promotes lateral bud redifferentiation with leaf sheath intersection growing point separate living tissue
It grows thickly.Subculture sum controlled in 15 generations.
Differentiation culture culture medium used, including following component: added on the basis of MS culture medium the basic element of cell division 3~
5mg/L obtains the KT-30 and appropriate auxin IBA and NAA of 6-BA, the TDZ of 0.004~0.015mg/L, 0.3~0.6mg/L,
Adjust the concentration (concentration of the main adjustment basic element of cell division) of above-mentioned hormone, the size and height of control differentiation sprout, so that increasing
Rate control is grown at 2.6 times.
(4) culture of rootage: root media used includes following component: MS+ sucrose 30g/L+IBA 0.1mg/L+NAA
0.5mg/L.Culture of rootage is packing container, 8 plants of more sturdy Bud Differentiations of the same size of every bag of inoculation with polypropylene film bag.
Promoting root growth vacation stem draws high culture, temperature control under dim light or no light condition indoors after being inoculated with Bud Differentiation with root media
System is at 25 DEG C -28 DEG C；Second week starts in greenhouse hardening, natural light shade irradiation, and intensity of illumination is controlled in 4000-5000lux；
Intensity of illumination is adjusted to 8000-10000lux after 2 weeks, until emergence.Tissue-cultured seedling stem enriches leaf dark green after 4 weeks, is migrated to
Motility rate is up to 96% or more.
Obtained miscellaneous No. 1 rooted seedling of powder, root system is more, more sturdy and white, seedling vacation stem chartreuse, and blade is dark green
Color, temporary planting reach 95% or more at the high survival rate of cup seedling (Fig. 2), and growth is very fast and neat, each explant (inhaling bud) 1 year
Can squamous subculture 13-15 generation, about 5000-8000 plants of seedling can be bred in total, after field planting kind property stablize, seldom there is variant,
Banana peasant's reflection is good.
Embodiment 3
Enforcement place: Fruit Tree Inst., Guangdong prov. Academy of Agricultural Sciences's maternal plant garden, the time: in October, 2014 starts.
(1) screening of maternal plant: select false stem it is green, it is sturdy, be highly 4.0m, fruit ear is larger, and fruit refers to that disjunctor fruit is less, fruit
The normal perennial root any of several broadleaf plants plant of shape, as stock tree, for taking bud to be used.
(2) explant inhales the processing of bud: when inhale bud it is long to 40cm when, add to bud spray 50% carbendazim, 1000 times of liquid are inhaled
The admixing medical solutions of 150ppm agricultural streptomycin, every bud fill 0.5 kilogram of admixing medical solutions；It is that explant is inoculated with that suction bud is taken after 1 week.
(3) differentiation culture: differentiation culture is packing container using vial, and temperature is controlled at 29 DEG C~33 DEG C.Differentiation training
Support 17 days progresss subcultures, subculture transfer Bud Differentiation when, the part top first sheathing leaf of Bud Differentiation is clipped in cutting, longitudinal sectional big bud bottom set and
Leaf sheath intersection growing point separate living tissue promotes lateral bud redifferentiation to grow thickly without separating bottom set to remove apical growth advantage.After
In generation, sum control was in 13 generations.
Differentiation culture culture medium used be added on the basis of MS culture medium 3~5mg/L obtain 6-BA, 0.004~
The KT-30 of the TDZ of 0.015mg/L, 0.3~0.6mg/L, and appropriate IBA and NAA, adjust hormone within the above range
Concentration, the size and height of control control differentiation sprout, so that proliferation rate control is at 2.8 times.
(4) culture of rootage and hardening: root media used includes following component: MS+ sucrose 30g/L+IBA0.2mg/L+
NAA0.7mg/L.Culture of rootage is packing container, 8 plants of more sturdy differentiation of the same size of every bag of inoculation with polypropylene film bag
Bud.
Dim light or the long rush seedling of unglazed promoting root growth, temperature are controlled at 25 DEG C -28 DEG C indoors；Greenhouse hardening, temperature are utilized after a week
Degree control is at 26 DEG C -30 DEG C, and natural light masking lower culture one week, intensity of illumination was controlled in 4000-5000lux；After two weeks, illumination
Intensity is adjusted to 8000-10000lux.Tissue-cultured seedling stem enriches leaf dark green after 4 weeks, and transplanting success is higher.
Miscellaneous No. 1 rooted seedling of powder bred with this technology, root system is more, more sturdy and white, seedling vacation stem chartreuse,
Dark green leaf is planted high survival rate, reaches 96%, and growth is very fast and neat, can breed about in each explant one-year age
6000~7000 plants of seedlings have bred nearly 800,000 plants altogether, and kind property is stablized after field planting, and yield is high, and fruit shape is good, variant about 1%, any of several broadleaf plants
Agriculture reflection is good.
Comparative example 1
Other methods with embodiment 1, the difference is that:
It is substituted using 3~4mg/L+IBA0.2mg/L of differential medium MS+6-BA of such as Brazilian any of several broadleaf plants of fragrant tooth any of several broadleaf plants of the invention
Differential medium carries out the culture of miscellaneous No. 1 dwarf banana of powder, the result is that Bud Differentiation proliferation rate is low, sprout directly grows upwards, long time cultivation
The miscellaneous No. 1 dwarf banana tissue-cultured seedling of powder only long root long leaf and it is undifferentiated.
Comparative example 2
Other methods with embodiment 1, the difference is that:
Life of the invention is substituted using banana prescription of rooting medium MS+ sucrose 20g/L+IBA1mg/L+NAA0.2mg/L
Root culture medium carries out the promoting root growth of miscellaneous No. 1 dwarf banana of powder, the result is that root is few and thick, it is lower that seedling is planted survival rate, sometimes only up to
20%。
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (3)
1. the tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder, characterized in that it comprises the following steps:
1) screen maternal plant: select false stem it is green, it is sturdy, highly be 3.2~4.5m, fruit ear is larger, and fruit refers to that disjunctor fruit is less, fruit shape
Normally, the good plant of quality is maternal plant；Wherein planting any of several broadleaf plants maternal plant height be 3.2~3.5m, perennial root any of several broadleaf plants maternal plant height be 3.8~
4.5m；
2) explant inhales the processing of bud: when inhale bud it is long to 30~50cm high when, to inhale bud spray 50% carbendazim, 1000 times of liquid and
The admixing medical solutions of the streptomysin of 150ppm concentration, it is that explant is inoculated with that suction bud is taken after 1 week；
3) differentiation culture: differential medium used is 3~5mg/L of addition on the basis of MS culture medium when differentiation culture
The KT-30 and appropriate IBA and NAA of 6-BA, the TDZ of 0.004~0.015mg/L and 0.3~0.6mg/L, control proliferation times
Number is 2.5~2.8 times；Break up cultivation temperature control at 29 DEG C~33 DEG C, 12~20 days progress subcultures, subculture sum are cultivated in differentiation
Control is in 12~16 generations；When subculture switching Bud Differentiation, Bud Differentiation part top first sheathing leaf, longitudinal sectional big bud bottom set and leaf are clipped in cutting
Sheath intersection growing point separate living tissue removes apical growth advantage, lateral bud redifferentiation is promoted to grow thickly without separating bottom set；
4) culture of rootage and hardening；It is inoculated with Bud Differentiation with root media, wherein root media is on the basis of MS culture medium
It is upper addition 30g/L sucrose, 0.1~0.2mg/L IBA and 0.5~0.8mg/L NAA；First week dim light or without striation indoors
Under part, the long seedling of promoting root growth；Second week utilizes greenhouse hardening, and temperature control is at 26-30 DEG C, and natural light shade is irradiated, intensity of illumination control
System is in 4000-5000lux；After two weeks, intensity of illumination is adjusted to 8000-10000lux.
2. tissue culture and rapid propagation method according to claim 1, it is characterised in that: in step 3), differentiation culture carries out for 15-18 days
Subculture, subculture sum controlled in 13~15 generations.
3. tissue culture and rapid propagation method according to claim 1, it is characterised in that: in step 4), with root media inoculation point
Change bud, the long seedling of promoting root growth, temperature control is at 25 DEG C -28 DEG C indoors under dim light or no light condition within first week；Second week utilizes greenhouse
Hardening, at 26 DEG C -30 DEG C, natural light shade irradiation, intensity of illumination is controlled in 4000-5000lux for temperature control；After 2 weeks, illumination
Intensity is adjusted to 8000-10000lux.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611123823.9A CN106688887B (en) | 2016-12-08 | 2016-12-08 | The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611123823.9A CN106688887B (en) | 2016-12-08 | 2016-12-08 | The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106688887A CN106688887A (en) | 2017-05-24 |
| CN106688887B true CN106688887B (en) | 2019-08-20 |
Family
ID=58936369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611123823.9A Active CN106688887B (en) | 2016-12-08 | 2016-12-08 | The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106688887B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107494278A (en) * | 2017-10-16 | 2017-12-22 | 李操 | A kind of quick-breeding method of Saigon any of several broadleaf plants tissue cultural seedlings of free |
| CN113142055B (en) * | 2021-04-29 | 2022-10-28 | 广西壮族自治区农业科学院 | In-vitro proliferation preservation method for banana germplasm resources |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726302A (en) * | 2012-07-18 | 2012-10-17 | 广西植物组培苗有限公司 | Dark culture method for banana tissue culture |
| CN103444535A (en) * | 2013-09-05 | 2013-12-18 | 广东省农业科学院果树研究所 | Novel method for increasing tissue culture and rapid propagation coefficients of banana |
| CN104982285A (en) * | 2015-06-17 | 2015-10-21 | 广东省农业科学院果树研究所 | Method for rapid propagation of banana seedlings based on suckers |
| CN106172011A (en) * | 2016-08-23 | 2016-12-07 | 广西壮族自治区农业科学院生物技术研究所 | A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method |
-
2016
- 2016-12-08 CN CN201611123823.9A patent/CN106688887B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726302A (en) * | 2012-07-18 | 2012-10-17 | 广西植物组培苗有限公司 | Dark culture method for banana tissue culture |
| CN103444535A (en) * | 2013-09-05 | 2013-12-18 | 广东省农业科学院果树研究所 | Novel method for increasing tissue culture and rapid propagation coefficients of banana |
| CN104982285A (en) * | 2015-06-17 | 2015-10-21 | 广东省农业科学院果树研究所 | Method for rapid propagation of banana seedlings based on suckers |
| CN106172011A (en) * | 2016-08-23 | 2016-12-07 | 广西壮族自治区农业科学院生物技术研究所 | A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method |
Non-Patent Citations (3)
| Title |
|---|
| "广粉1号粉蕉组培快繁技术研究";王丽萍等;《中国园艺文摘》;20151231(第3期);第12-13、122页 * |
| "粉蕉离体的快速繁殖技术";吕庆芳;《湛江海洋大学学报》;20030831;第23卷(第4期);第57-61页 * |
| "粉蕉试管苗工厂化生产中的光温调控";梁钾贤;《中国农学通报》;20111231;第27卷(第25期);第283-287页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106688887A (en) | 2017-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109006473B (en) | Kiwi fruit tissue culture rapid propagation culture medium and method | |
| CN104663450A (en) | Tissue culture and rapid propagation method for Acer rubrum 'Brandywine' | |
| CN105010143B (en) | A kind of extracorporeal culturing method of Chinese catalpa | |
| KR20150026554A (en) | Development of a micropropagation technique in Prunus avium clones | |
| US20220174892A1 (en) | Tree eggplant and cultivation method, rapid propagation method and application thereof | |
| CN102805035A (en) | Common head cabbage tissue culture method | |
| CN103430844A (en) | Method for gardenia tissue culture | |
| CN103548692B (en) | A kind of tissue culture and rapid propagation method of ripple leaf new pteris fern seedling | |
| CN102907326B (en) | Tissue culture propagation method for Medicagao Sativa L. | |
| JPS5914725A (en) | Production of plant propagating material | |
| KR20110113918A (en) | Method for plant formation of blueberry through stem tip culture | |
| CN106688887B (en) | The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder | |
| CN105145363B (en) | It is a kind of to significantly improve the method that China fir tissue culture produces emergence rate | |
| CN107079817A (en) | Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method | |
| CN109258478A (en) | The tissue culture propagation method of polygonatum cyrtonema | |
| CN105519448A (en) | Culture method of radix astragali tissue culture seedlings | |
| Gami et al. | Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.) cultivar K. badshah | |
| CN105028193A (en) | Breeding method for generating micro adventitious buds through induction of legacy leaves | |
| CN102119663A (en) | Tissue culture quick propagation technology of clematis mademe Julia correvon | |
| CN103563747A (en) | Detoxification and rapid-propagation method of huilou yam | |
| CN103503771A (en) | Tissue culture and rapid propagation method for Australian hardenbergia violacea seedlings | |
| CN106605596B (en) | A method of mass propagation Lycoris aurea is occurred by body embryo | |
| KR101281934B1 (en) | Nado Lantern New breed Prince | |
| CN112616675B (en) | Tissue culture and rapid propagation method for Zingiber dance | |
| CN107306791A (en) | A kind of Huangshi dry measure used in former times seedling quick breeding method for tissue culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |