CN106676091A - Preparation method of immobilized microorganism spherules - Google Patents
Preparation method of immobilized microorganism spherules Download PDFInfo
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- CN106676091A CN106676091A CN201510748106.4A CN201510748106A CN106676091A CN 106676091 A CN106676091 A CN 106676091A CN 201510748106 A CN201510748106 A CN 201510748106A CN 106676091 A CN106676091 A CN 106676091A
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Abstract
A preparation method of immobilized microorganism spherules comprises the following steps: 1, preparing gel; 2, preparing a cross-linking agent: cross-linking a saturated boric acid solution for 12 h, filtering out particles, transferring the filtered solution to a 1mol/L NaH2PO4 solution, and carrying out continuous cross-linking for 2 h; 3, slowly stirring the cross-linking agent at a rotating speed of 125-140 r/min; 4, immobilizing: dropwise adding the uniformly mixed gel to the cooled stirred cross-linking agent by using a peristaltic pump to form white spherical spherules with the diameter of about 2 mm, standing the white spherical spherules, uniformly mixing the white spherical spherules by a magnetic stirrer, immersing the white spherical spherules at 4 DEG C for 24-48 h, heating for dissolving a polyvinyl alcohol and sodium alginate mixed solution in the immobilization process, and standing mixed solution at room temperature for 4 h or above; 5, controlling the dropwise adding speed of the peristaltic pump; and 6, cross-linking prepared immobilized particles in the cross-linking agent for 24 h to guarantee the strength, wherein the immobilized microorganism spherules are flushed with normal saline which is a 0.9% NaCl solution before being used. The immobilized microorganism spherules have the advantages of low production cost, easiness in spherule formation, no tailing and moderate viscosity, and the preparation method has the advantages of simplicity, convenience, and easiness in mass production.
Description
Technical field
The present invention relates to a kind of microorganism remediation liquid, particularly a river water quality and riverbed probiotic microorganisms repair liquid.
Background technology
Improve water quality, need to solve in terms of two.First, throttle.Advocate thriftiness and use water, make full use of sewage reuse techuique, effectively reduce the discharge capacity of sewage.2nd, water body self-purification ability is improved.Microbial bacterial agent technology is exactly a kind of method therein.Microbial bacterial agent technology, mean the high-efficiency strain by the dominant bacteria with specific function being added in Waste Water Treatment or produce using gene recombination technology, it is set to act synergistically with indigneous flora, the ecosystem is improved to pollutant and the removal ability of harmful substance, the performance of the optimization ecosystem, so as to realize the depollution of environment.Since 20 century 70s, some composite bacteria agents have all been succeeded in developing in Europe, the United States, Japan and other countries or area in succession, much have begun to be produced on a large scale, and define the product of seriation, mainly for the treatment of the improvement of sanitary sewage, industrial and agricultural wastewater and the pollution of some other compound.Wherein by Japanese university of Ryukyu than praise according to husband teach in phase early 1980s develop EM (CEM) microbial inoculum be most successful, in more than 90 countries uses in terms of planting industry, aquaculture and the depollution of environment, significant economic, ecological and social benefit is generated.But, because past microbial bacterial agent is expensive, microbial inoculum action time is short, and microbial bacterial agent technology is often cured the symptoms, not the disease in water remediation.Therefore, microbial bacterial agent technology is not widely used in China.
Nowadays, with the development of microbial inoculum technology of preparing, microbial inoculum cost is greatly reduced, and by microbial inoculum immobilization technology, extends the effective acting time of microbial inoculum, and to the research of microbial bacterial agent technology new opportunity is brought.
The content of the invention
The technical problem to be solved in the present invention is, for the deficiencies in the prior art, to propose a kind of preparation method of immobilized microorganism bead.
The technical problem to be solved in the present invention is achieved through the following technical solutions.The present invention is a kind of preparation method of immobilized microorganism bead, is characterized in, is comprised the following steps：
（1）It is prepared by gel, weigh a certain amount of PVA and sodium alginate is added in 100ml water, it is completely dissolved at 85 DEG C with thermostat water bath after infiltration 24h, it is that 0.5%, attapulgite powder concentration is 3.5% that additive SiO2 concentration is subsequently adding for 0.6%, CaCO3 concentration, stir, room temperature is cooled to, a certain amount of microbial inoculum is added；
（2）It is prepared by crosslinking agent：Boric acid, CaCl2 are weighed respectively to dissolve at room temperature, 25% glutaraldehyde water solution is added, and make boric acid saturation for 6.5g g/mL, saturation boric acid and 2% CaCl2 mixed solutions, crosslinking 12h；
（3）Particle is leached after saturation BAS crosslinking 12h, is transposed in the NaH2PO4 solution of 1mol/L and is continued to be crosslinked 2h；
（4）By step（3）Crosslinking agent is slowly stirred in rotating speed for 125 ~ 140 revs/min；
（5）Compounding, different nutrient solution COD solution weighs 1.031g glucose, deionized water dissolving, for being embedded in immobilization particle used in；
（6）Process for fixation, the gel peristaltic pump being well mixed is added drop-wise in the crosslinking agent that the stirring of cooling is completed, form it into the white ball-type bead of about 2mm diameters, standing is mixed with magnetic stirring apparatus, 24-48h is soaked at 4 DEG C, in immobilization process, by polyvinyl alcohol and mixed solution of sodium alginate heating for dissolving, more than 4h is stood at room temperature；Peristaltic pump rate of addition is controlled simultaneously；
（7）Obtained immobilization particle is crosslinked into 24h in crosslinking agent, it is ensured that its intensity, is rinsed using front use physiological saline 0.9%NaCl solution.
In a kind of preparation method of immobilized microorganism bead of the present invention, further preferred technical scheme is characterized in that：
1st, the PVA concentration is 8%；
2nd, the sodium alginate quality is 0.05 ~ 0.1g, and bacterium solution volume is in 0.05 ~ 0.1 ml.
Compared with prior art, the present invention effectively removes the elements such as the nitrogen of river bed by immobilized microorganism technique, obtained bead has good biologically active and hydraulic intensity, material is cheap, and low production cost, balling-up is easy, do not trail, modest viscosity, preparation method is simple and convenient, it is easy to quantify production.
Specific embodiment
The concrete technical scheme of the present invention is further described, in order to those skilled in the art the present invention is further understood that, and do not constitute the restriction of its power.
Embodiment 1, the present invention is a kind of preparation method of immobilized microorganism bead, is characterized in, is comprised the following steps：
（1）It is prepared by gel, weigh a certain amount of PVA and sodium alginate is added in 100ml water, it is completely dissolved at 85 DEG C with thermostat water bath after infiltration 24h, it is that 0.5%, attapulgite powder concentration is 3.5% that additive SiO2 concentration is subsequently adding for 0.6%, CaCO3 concentration, stir, room temperature is cooled to, a certain amount of microbial inoculum is added；
（2）It is prepared by crosslinking agent：Boric acid, CaCl2 are weighed respectively to dissolve at room temperature, 25% glutaraldehyde water solution is added, and make boric acid saturation for 6.5g g/mL, saturation boric acid and 2% CaCl2 mixed solutions, crosslinking 12h；
（3）Particle is leached after saturation BAS crosslinking 12h, is transposed in the NaH2PO4 solution of 1mol/L and is continued to be crosslinked 2h；
（4）By step（3）Crosslinking agent is slowly stirred in rotating speed for 125 ~ 140 revs/min；
（5）Compounding, different nutrient solution COD solution weighs 1.031g glucose, deionized water dissolving, for being embedded in immobilization particle used in；
（6）Process for fixation, the gel peristaltic pump being well mixed is added drop-wise in the crosslinking agent that the stirring of cooling is completed, form it into the white ball-type bead of about 2mm diameters, standing is mixed with magnetic stirring apparatus, 24-48h is soaked at 4 DEG C, in immobilization process, by polyvinyl alcohol and mixed solution of sodium alginate heating for dissolving, more than 4h is stood at room temperature；Peristaltic pump rate of addition is controlled simultaneously；
（7）Obtained immobilization particle is crosslinked into 24h in crosslinking agent, it is ensured that its intensity, is rinsed using front use physiological saline 0.9%NaCl solution.
Embodiment 2, a kind of preparation method of the immobilized microorganism bead described in embodiment 1, the PVA concentration is 8%.
Embodiment 3, a kind of preparation method of the immobilized microorganism bead described in embodiment 1, the sodium alginate quality is 0.05 ~ 0.1g, and bacterium solution volume is in 0.05 ~ 0.1 ml.
Claims (3)
1. a kind of preparation method of immobilized microorganism bead, it is characterised in that comprise the following steps：
（1）It is prepared by gel, weigh a certain amount of PVA and sodium alginate is added in 100ml water, it is completely dissolved at 85 DEG C with thermostat water bath after infiltration 24h, it is that 0.5%, attapulgite powder concentration is 3.5% that additive SiO2 concentration is subsequently adding for 0.6%, CaCO3 concentration, stir, room temperature is cooled to, a certain amount of microbial inoculum is added；
（2）It is prepared by crosslinking agent：Boric acid, CaCl2 are weighed respectively to dissolve at room temperature, 25% glutaraldehyde water solution is added, and make boric acid saturation for 6.5g g/mL, saturation boric acid and 2% CaCl2 mixed solutions, crosslinking 12h；
（3）Particle is leached after saturation BAS crosslinking 12h, is transposed in the NaH2PO4 solution of 1mol/L and is continued to be crosslinked 2h；
（4）By step（3）Crosslinking agent is slowly stirred in rotating speed for 125 ~ 140 revs/min；
（5）Compounding, different nutrient solution COD solution weighs 1.031g glucose, deionized water dissolving, for being embedded in immobilization particle used in；
（6）Process for fixation, the gel peristaltic pump being well mixed is added drop-wise in the crosslinking agent that the stirring of cooling is completed, form it into the white ball-type bead of about 2mm diameters, standing is mixed with magnetic stirring apparatus, 24-48h is soaked at 4 DEG C, in immobilization process, by polyvinyl alcohol and mixed solution of sodium alginate heating for dissolving, more than 4h is stood at room temperature；Peristaltic pump rate of addition is controlled simultaneously；
（7）Obtained immobilization particle is crosslinked into 24h in crosslinking agent, it is ensured that its intensity, is rinsed using front use physiological saline 0.9%NaCl solution.
2. the preparation method of a kind of immobilized microorganism bead according to claim 1, it is characterised in that：The PVA concentration is 8%.
3. river water quality according to claim 1 and riverbed probiotic microorganisms repair liquid, it is characterised in that：The sodium alginate quality is 0.05 ~ 0.1g, and bacterium solution volume is in 0.05 ~ 0.1 ml.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220199A (en) * | 2018-02-06 | 2018-06-29 | 浦江县欧立生物技术有限公司 | A kind of preparation method of complex microbial inoculum |
| CN108531525A (en) * | 2018-05-09 | 2018-09-14 | 宿州学院 | A kind of enzymatic conversion preparation method of L-Aspartic acid |
| CN110124618A (en) * | 2019-04-15 | 2019-08-16 | 华南农业大学 | A kind of hydrotalcite-plant cellulose composite pellets and the preparation method and application thereof |
| CN110452900A (en) * | 2019-08-26 | 2019-11-15 | 天津科技大学 | A kind of preparation method for the compound fixation support of PVA-SA embedding degrading microorganism |
| CN114438070A (en) * | 2022-02-15 | 2022-05-06 | 广东海纳川生物科技股份有限公司 | Suspended solid microorganism, preparation method thereof, culture pond and application |
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| CN101875928A (en) * | 2009-04-29 | 2010-11-03 | 中国环境科学研究院 | Embedding immobilization method for microbial preparation |
| CN103013973A (en) * | 2012-12-17 | 2013-04-03 | 常州大学 | Method for preparing aerobiotic nitrifier immobilizing vector |
| CN103614360A (en) * | 2013-11-18 | 2014-03-05 | 北京工业大学 | Method for inhibiting photosynthetic bacteria of algae by immobilizing composite embedding medium |
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- 2015-11-06 CN CN201510748106.4A patent/CN106676091A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101875928A (en) * | 2009-04-29 | 2010-11-03 | 中国环境科学研究院 | Embedding immobilization method for microbial preparation |
| CN103013973A (en) * | 2012-12-17 | 2013-04-03 | 常州大学 | Method for preparing aerobiotic nitrifier immobilizing vector |
| CN103614360A (en) * | 2013-11-18 | 2014-03-05 | 北京工业大学 | Method for inhibiting photosynthetic bacteria of algae by immobilizing composite embedding medium |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220199A (en) * | 2018-02-06 | 2018-06-29 | 浦江县欧立生物技术有限公司 | A kind of preparation method of complex microbial inoculum |
| CN108531525A (en) * | 2018-05-09 | 2018-09-14 | 宿州学院 | A kind of enzymatic conversion preparation method of L-Aspartic acid |
| CN110124618A (en) * | 2019-04-15 | 2019-08-16 | 华南农业大学 | A kind of hydrotalcite-plant cellulose composite pellets and the preparation method and application thereof |
| CN110124618B (en) * | 2019-04-15 | 2020-04-17 | 华南农业大学 | Hydrotalcite-plant cellulose composite pellet and preparation method and application thereof |
| CN110452900A (en) * | 2019-08-26 | 2019-11-15 | 天津科技大学 | A kind of preparation method for the compound fixation support of PVA-SA embedding degrading microorganism |
| CN114438070A (en) * | 2022-02-15 | 2022-05-06 | 广东海纳川生物科技股份有限公司 | Suspended solid microorganism, preparation method thereof, culture pond and application |
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