CN106668946A - Carbon nano-tube/chitosan/collagen composite scaffold material and preparation thereof - Google Patents
Carbon nano-tube/chitosan/collagen composite scaffold material and preparation thereof Download PDFInfo
- Publication number
- CN106668946A CN106668946A CN201611126200.7A CN201611126200A CN106668946A CN 106668946 A CN106668946 A CN 106668946A CN 201611126200 A CN201611126200 A CN 201611126200A CN 106668946 A CN106668946 A CN 106668946A
- Authority
- CN
- China
- Prior art keywords
- chitosan
- collagen
- support frame
- carbon nano
- frame material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/443—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with carbon fillers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
Abstract
The invention belongs to the field of tissue engineering materials, and in particular relates to a carbon nano-tube/chitosan/collagen composite scaffold material and preparation thereof. The preparation comprises the following steps: mixing high-concentration chitosan, a collagen solution and a carbon nano-tube, and performing the cross-linked self-assembly, wherein the obtained compound gathers respective advantages of the carbon nano-tube, the chitosan and the collagen after freeze-drying, and has excellent low antigenicity, low irritation and low cytotoxicity, and is capable of promoting cell growth and mechanical property; the carbon nano-tube/chitosan/collagen composite scaffold material is of a three-dimensional porous structure, is provided with multiple pores, has large specific surface area, and is capable of further carrying the medicine or cell growth factors, thereby achieving a slow-release effect.
Description
Technical field
The invention belongs to tissue engineering material field, more particularly to a kind of Carbon Nanotubes/Chitosan/collagen composite support
Frame material and its preparation.
Background technology
Shitosan, also known as chitosan, is that the chitin being widely present by nature is obtained by deacetylation
, chemical name is Chitosan (1-4) -2- amino-B-D glucose, and shitosan possesses excellent biological functionality and phase
Capacitive, blood compatibility, security, microbic resolvability etc., are widely used in biological medicine, food, chemical industry, cosmetics, water
The numerous areas such as treatment, METAL EXTRACTION and recovery.Under given conditions, shitosan can hydrolyze, be alkylated, being acylated,
The chemical reactions such as carboxy methylation, sulfonation, nitrification, halogenation, oxidation, reduction, condensation and complexing, can generate various with different performance
Chitosan derivatives, so as to expand the range of application of shitosan.
CNT is lightweight as monodimension nanometer material, and hexagonal structure connection is perfect, with many abnormal power
, electricity and chemical property.And used as one kind of carbon material, CNT also possesses excellent biocompatibility.In general,
SWCN has chemical inertness higher, and its surface wants purer, and multi-wall carbon nano-tube pipe surface will vivaciously much,
It is combined with substantial amounts of surface group, such as carboxyl.CNT has a good mechanical property, and tensile strength reaches 50~
200GPa, is 100 times of steel, and density but only has the 1/6 of steel, an order of magnitude at least higher than ordinary graphite fiber；Its elasticity
Modulus is up to 1TPa, and the elastic modelling quantity with diamond is suitable, about 5 times of steel.
Collagen is boiomacromolecule, the main component in animal connective tissue, be also mammal in-vivo content most
The many, functional protein that distribution is most wide, collagen is because living with good biocompatibility, biodegradable and biology
Property, therefore be widely applied in fields such as food, medicine, organizational project, cosmetics.
It is the three-dimensional porous knot of cell growth conveying nutrition and excretion metabolism product in organizational project that biological support refers to
The cell carrier of structure.Therefore timbering material usually requires that possess good biocompatibility, three-dimensional porous structure and certain power
Learn performance.
Biologic bracket material of the prior art is divided into natural material and synthetic material, and natural material often has excellent
Good biocompatibility, but mechanical property has often been short of, and synthetic material biocompatibility is not good enough, or do not possess
Adaptable mechanical property.
The content of the invention
The present invention is to solve that biologic bracket material biocompatibility in the prior art is bad, the not good technology of mechanical property
A kind of problem, there is provided compound support frame material for having superior bio compatibility, three-dimensional porous structure and mechanical property concurrently.
To solve the above problems, technical scheme is as follows：
A kind of Carbon Nanotubes/Chitosan/collagen compound support frame material, is made up of the raw material of following weight portion：
Shitosan：35-48 parts；
Collagen：35-48 parts；
CNT：4-30 parts.
Preferably, the CNT is multi-walled carbon nano-tubes, and a diameter of 20-40 nm, length is 2-5 microns.
Shitosan have biology can battle array solution, Bc, biological non-toxicity the features such as, but mechanical property is low；Collagen
Albumen possesses excellent biodegradability, biocompatibility, low antigenicity, low irritant and low cytotoxicity, and promotes
The performances such as cell growth, but there is also the low problem of mechanical strength；Multi-walled carbon nano-tubes possesses excellent mechanical property and biology
Compatibility；The shitosan of high concentration, collagen solution and a small amount of CNT be compound, and its is excellent for obtained composite set
Point, with excellent low antigenicity, low irritant and low cytotoxicity, and promotes cell growth and mechanical property.
Preferably, the Carbon Nanotubes/Chitosan/collagen compound support frame material, by the raw material group of following weight portion
Into：
Shitosan：42 parts；
Collagen：42 parts；
CNT：8 parts.
A kind of preparation method of Carbon Nanotubes/Chitosan/collagen compound support frame material, comprises the following steps：
A. in dissolving the chitosan in the acetum that concentration is 1%；
B. by multi-walled carbon nano-tubes add 15 mg/mL cetyl trimethylammonium bromide solutions in, be well mixed after, ultrasound 4
Hour, multi-walled carbon nano-tubes concentration is 1-4 mg/mL；
C. step A, B is obtained dissolving mixing, ultrasound 3 hours；
D. collagen is dissolved in the mixed solution of step C, is stirred 24 hours under the conditions of 35 DEG C of water-bath；
E. by mixed solution freeze-drying obtained in step D, Carbon Nanotubes/Chitosan/collagen compound support frame material is obtained.
The CNT of the shitosan, collagen solution and cetyl trimethylammonium bromide modification of high concentration possesses good
Good adhesion, can crosslink and self assembly after mixing.
Preferably, appropriate crosslinking agent is added in mixed solution described in step D so that shitosan, collagen solution and carbon
Nanotube is preferably crosslinked, and forms the more stable compound of structure.
Preferably, the crosslinking agent, it is characterised in that glutaraldehyde, Carbodiimides or alkylene oxide base class crosslinking agent, hands over
The mass concentration for joining solution is 0.1%-1%.
Preferably, the lyophilisation condition of the step E is：Under vacuum condition, temperature is -140 DEG C -- 100 DEG C, drying time
6-12 hours.After lyophilized, products therefrom is three-dimensional porous structure, and hole is more, and specific surface area is big, can further carry medicine or
Porcine HGF, reaches the effect of sustained release.
Relative to prior art, advantages of the present invention is as follows, a kind of Carbon Nanotubes/Chitosan/collagen of the invention
Compound support frame material set CNT, shitosan and the respective advantage of collagen, with excellent low antigenicity, low stimulation
Property and low cytotoxicity, and promote cell growth and mechanical property, the shitosan of high concentration, collagen solution and carbon nanometer
For good adhesion, the lyophilized product for obtaining is three-dimensional porous structure to pipe, and hole is more, and specific surface area is big, can further take
Medicine or Porcine HGF are carried, the effect of sustained release is reached.
Specific embodiment
Embodiment 1：
A kind of Carbon Nanotubes/Chitosan/collagen compound support frame material, is made up of the raw material of following weight portion：
Shitosan：35-48 parts；
Collagen：35-48 parts；
CNT：4-30 parts.
Preferably, the CNT is multi-walled carbon nano-tubes, and a diameter of 20-40 nm, length is 2-5 microns.
Shitosan have biology can battle array solution, Bc, biological non-toxicity the features such as, but mechanical property is low；Collagen
Albumen possesses excellent biodegradability, biocompatibility, low antigenicity, low irritant and low cytotoxicity, and promotes
The performances such as cell growth, but there is also the low problem of mechanical strength；Multi-walled carbon nano-tubes possesses excellent mechanical property and biology
Compatibility；The shitosan of high concentration, collagen solution and a small amount of CNT be compound, and its is excellent for obtained composite set
Point, with excellent low antigenicity, low irritant and low cytotoxicity, and promotes cell growth and mechanical property.
Embodiment 2：
A kind of Carbon Nanotubes/Chitosan/collagen compound support frame material, is made up of the raw material of following weight portion：
Shitosan：42 parts；
Collagen：42 parts；
CNT：8 parts.
Embodiment 3：
A kind of preparation method of Carbon Nanotubes/Chitosan/collagen compound support frame material, comprises the following steps：
A. in dissolving the chitosan in the acetum that concentration is 1%；
B. by multi-walled carbon nano-tubes add 15 mg/mL cetyl trimethylammonium bromide solutions in, be well mixed after, ultrasound 4
Hour, multi-walled carbon nano-tubes concentration is 1-4 mg/mL；
C. step A, B is obtained dissolving mixing, ultrasound 3 hours；
D. collagen is dissolved in the mixed solution of step C, is stirred 24 hours under the conditions of 35 DEG C of water-bath；
E. by mixed solution freeze-drying obtained in step D, Carbon Nanotubes/Chitosan/collagen compound support frame material is obtained.
The CNT of the shitosan, collagen solution and cetyl trimethylammonium bromide modification of high concentration possesses good
Good adhesion, can crosslink and self assembly after mixing.
Preferably, appropriate crosslinking agent is added in mixed solution described in step D so that shitosan, collagen solution and carbon
Nanotube is preferably crosslinked, and forms the more stable compound of structure.
Preferably, the crosslinking agent, it is characterised in that glutaraldehyde, Carbodiimides or alkylene oxide base class crosslinking agent, hands over
The mass concentration for joining solution is 0.1%-1%.
Preferably, the lyophilisation condition of the step E is：Under vacuum condition, temperature is -140 DEG C -- 100 DEG C, drying time
6-12 hours.After lyophilized, products therefrom is three-dimensional porous structure, and hole is more, and specific surface area is big, can further carry medicine or
Porcine HGF, reaches the effect of sustained release.
It should be noted that above-described embodiment is only presently preferred embodiments of the present invention, it is not of the invention for limiting
Protection domain, the equivalent made on the basis of the above or replacement belong to protection scope of the present invention.
Claims (6)
1. a kind of Carbon Nanotubes/Chitosan/collagen compound support frame material, it is characterised in that by the raw material of following weight portion
Composition：
Shitosan：35-48 parts；
Collagen：35-48 parts；
CNT：4-30 parts.
2. Carbon Nanotubes/Chitosan as claimed in claim 1/collagen compound support frame material, it is characterised in that the carbon
Nanotube is multi-walled carbon nano-tubes, a diameter of 20-40 nm, and length is 2-5 microns.
3. the preparation method of a kind of Carbon Nanotubes/Chitosan/collagen compound support frame material, it is characterised in that including following
Step：
A. in dissolving the chitosan in the acetum that concentration is 1%；
B. by multi-walled carbon nano-tubes add 15 mg/mL cetyl trimethylammonium bromide solutions in, be well mixed after, ultrasound 4
Hour, multi-walled carbon nano-tubes concentration is 2-15 mg/mL；
C. step A, B is obtained dissolving mixing, ultrasound 3 hours；
D. collagen is dissolved in the mixed solution of step C, is stirred 24 hours under the conditions of 35 DEG C of water-bath；
E. by mixed solution freeze-drying obtained in step D, Carbon Nanotubes/Chitosan/collagen compound support frame material is obtained.
4. the preparation method of Carbon Nanotubes/Chitosan as claimed in claim 3/collagen compound support frame material, its feature
It is to add crosslinking agent in mixed solution described in step D.
5. the preparation method of Carbon Nanotubes/Chitosan as claimed in claim 4/collagen compound support frame material, its feature
It is, the crosslinking agent, it is characterised in that glutaraldehyde, Carbodiimides or alkylene oxide base class crosslinking agent, the matter of crosslinker solution
Amount concentration is 0.1%-1%.
6. the preparation method of Carbon Nanotubes/Chitosan as claimed in claim 5/collagen compound support frame material, its feature
It is that the lyophilisation condition of the step E is：Under vacuum condition, temperature is -140 DEG C -- 100 DEG C, drying time 6-12 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611126200.7A CN106668946A (en) | 2016-12-09 | 2016-12-09 | Carbon nano-tube/chitosan/collagen composite scaffold material and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611126200.7A CN106668946A (en) | 2016-12-09 | 2016-12-09 | Carbon nano-tube/chitosan/collagen composite scaffold material and preparation thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106668946A true CN106668946A (en) | 2017-05-17 |
Family
ID=58867796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611126200.7A Pending CN106668946A (en) | 2016-12-09 | 2016-12-09 | Carbon nano-tube/chitosan/collagen composite scaffold material and preparation thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106668946A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107537063A (en) * | 2017-07-17 | 2018-01-05 | 大连理工大学 | A kind of complex stephanoporate bracket of carbon nanotubes and preparation method thereof |
| CN107737376A (en) * | 2017-10-31 | 2018-02-27 | 无锡中科光远生物材料有限公司 | A kind of preparation method of conductive collagen cardiac patch |
| CN110801513A (en) * | 2019-12-17 | 2020-02-18 | 河南汇博医疗股份有限公司 | Composite sustained-release particle loaded with growth factor and preparation method thereof |
| CN115124987A (en) * | 2022-06-13 | 2022-09-30 | 陕西延长石油（集团）有限责任公司 | Long-acting hypersalinity-resistant low-temperature-resistant solid foam scrubbing agent for natural gas ground gathering and transportation pipeline and preparation method thereof |
| CN115715824A (en) * | 2022-10-12 | 2023-02-28 | 杭州洛兮生物科技有限公司 | Bone scaffold material based on modified single-walled carbon nanotubes and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103196965A (en) * | 2013-03-18 | 2013-07-10 | 北京科技大学 | Method for preparing carbon nanotube composite conductive hydrogel coating modified electrode |
| CN104147643A (en) * | 2014-08-06 | 2014-11-19 | 江苏双林海洋生物药业有限公司 | Method for preparing conductive tissue engineering scaffold of chitosan and carbon nanometer tube |
-
2016
- 2016-12-09 CN CN201611126200.7A patent/CN106668946A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103196965A (en) * | 2013-03-18 | 2013-07-10 | 北京科技大学 | Method for preparing carbon nanotube composite conductive hydrogel coating modified electrode |
| CN104147643A (en) * | 2014-08-06 | 2014-11-19 | 江苏双林海洋生物药业有限公司 | Method for preparing conductive tissue engineering scaffold of chitosan and carbon nanometer tube |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107537063A (en) * | 2017-07-17 | 2018-01-05 | 大连理工大学 | A kind of complex stephanoporate bracket of carbon nanotubes and preparation method thereof |
| CN107537063B (en) * | 2017-07-17 | 2019-10-11 | 大连理工大学 | A kind of complex stephanoporate bracket of carbon nanotubes and preparation method thereof |
| CN107737376A (en) * | 2017-10-31 | 2018-02-27 | 无锡中科光远生物材料有限公司 | A kind of preparation method of conductive collagen cardiac patch |
| CN110801513A (en) * | 2019-12-17 | 2020-02-18 | 河南汇博医疗股份有限公司 | Composite sustained-release particle loaded with growth factor and preparation method thereof |
| CN115124987A (en) * | 2022-06-13 | 2022-09-30 | 陕西延长石油（集团）有限责任公司 | Long-acting hypersalinity-resistant low-temperature-resistant solid foam scrubbing agent for natural gas ground gathering and transportation pipeline and preparation method thereof |
| CN115715824A (en) * | 2022-10-12 | 2023-02-28 | 杭州洛兮生物科技有限公司 | Bone scaffold material based on modified single-walled carbon nanotubes and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106668946A (en) | Carbon nano-tube/chitosan/collagen composite scaffold material and preparation thereof | |
| Pei et al. | Applications of carbon nanotubes in bone tissue regeneration and engineering: Superiority, concerns, current advancements, and prospects | |
| CN101235193B (en) | Method for preparing degradable biocompatibility macromolecule/carbon nano-tube composite material | |
| Gopi et al. | General scenarios of cellulose and its use in the biomedical field | |
| Chang et al. | A review on the properties and applications of chitosan, cellulose and deep eutectic solvent in green chemistry | |
| de Menezes et al. | Recent advances in the use of carbon nanotubes as smart biomaterials | |
| Ding et al. | Emerging chitin and chitosan nanofibrous materials for biomedical applications | |
| Pangon et al. | Hydroxyapatite-hybridized chitosan/chitin whisker bionanocomposite fibers for bone tissue engineering applications | |
| Zhou et al. | Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering | |
| Stanisławska | Bacterial nanocellulose as a microbiological derived nanomaterial | |
| Naskar et al. | Carbon nanofiber reinforced nonmulberry silk protein fibroin nanobiocomposite for tissue engineering applications | |
| Xu et al. | Chitin nanocrystal enhanced wet adhesion performance of mussel-inspired citrate-based soft-tissue adhesive | |
| Xu et al. | Intrinsically water-stable keratin nanoparticles and their in vivo biodistribution for targeted delivery | |
| Sadat et al. | A comprehensive review on the applications of carbon-based nanostructures in wound healing: from antibacterial aspects to cell growth stimulation | |
| Şen et al. | Boron nitride nanotubes included thermally cross-linked gelatin–glucose scaffolds show improved properties | |
| Lim et al. | Preparation of interconnected porous chitosan scaffolds by sodium acetate particulate leaching | |
| CN101612128B (en) | Alginic acid inorganic nanometer composite gel microspheres and preparation method | |
| Geng et al. | Achieving stem cell imaging and osteogenic differentiation by using nitrogen doped graphene quantum dots | |
| Sivanesan et al. | Reviewing chitin/chitosan nanofibers and associated nanocomposites and their attained medical milestones | |
| CN102344686A (en) | Method for preparing fibroin nanoparticles taking polyvinyl alcohol as stabilizer | |
| Ahuja et al. | Microbial exopolysaccharide composites in biomedicine and healthcare: trends and advances | |
| Heydari et al. | Fabrication of multicomponent cellulose/polypyrrole composed with zinc oxide nanoparticles for improving mechanical and biological properties | |
| CN103013140B (en) | Carbon nano tube/collagen based composite material and preparation method thereof | |
| Srinivasan | Carbon nanotubes in cancer therapy | |
| Cho et al. | Stem cell response to multiwalled carbon nanotube-incorporated regenerated silk fibroin films |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170517 |
|
| RJ01 | Rejection of invention patent application after publication |